AT2G21100

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003188
Physical Location & Seq
Reverse (-)
9048131 .. 9049404
1274 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G21100.2

Sequence Viewer

Length: 564 bp
ATGGCAAAAGAGGAATATGTATCAAGAATGTTGGTTATGTTAATAATGATAATGCCACTGGTTGCTCAGGGATCGAGACTGCATAGTTGGGCTAATCGGCTTGAGGAAACAGGGAAGGACAAGGTAACAAACCTTCAGTTCTATTTCCATGACACCTTGAGCGGCAAGAATCCAACCGCAGTTAAGGTGGCTCAGGGCACAGACACCGAAAAATCCCCGACGCTGTTCGGCGCTGTGTTCATGGTCGACGACGCTCTCACCGAGACCGCTGATCCAAAATCCAAACTCGTTGGACGGGCGCAAGGCCTTTACGGTTCTTCATGTAAAGAAGAAGTAGGTTTGATAATGGCCATGAGCTTCTGTTTCGAGGACGGTCCTTACAAGGACAGCACCATAAGCATGATCGGGAAGAACTCCGCGATGAACCCAATCCGAGAAATGCCTATTGTTGGCGGCACGGGAATGTTCCGGATGGCTCGTGGCTATGCTATTGCCCGGACTAATTGGTTTGATCCCAAAACCGGTGATGCCATCGTTGGCTATAACGTTACCATTATGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

187

Amino Acids

20.59

Weight (kDa)

6.83

Isoelectric Point (pI)

26.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 42 - 185 5.5e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 162
AccI GTMKAC 1 cut(s) 246
AccII CGCG 1 cut(s) 419
AccIII TCCGGA 1 cut(s) 468
AciI CCGC 5 cut(s) 162, 177, 267, 417, 453
AclI AACGTT 1 cut(s) 546
AclWI GGATC 3 cut(s) 79, 266, 506
AcoI YGGCCR 1 cut(s) 348
AcuI CTGAAG 1 cut(s) 119
AfiI CCNNNNNNNGG 2 cut(s) 449, 521
AgeI ACCGGT 1 cut(s) 521
AluBI AGCT 1 cut(s) 357
AluI AGCT 1 cut(s) 357
Alw26I GTCTC 2 cut(s) 70, 257
AlwI GGATC 3 cut(s) 79, 266, 506
Aor13HI TCCGGA 1 cut(s) 468
AoxI GGCC 2 cut(s) 304, 348
AsiGI ACCGGT 1 cut(s) 521
AspLEI GCGC 2 cut(s) 233, 301
AspS9I GGNCC 1 cut(s) 374
AsuC2I CCSGG 1 cut(s) 496
AsuHPI GGTGA 2 cut(s) 250, 536
AvaII GGWCC 1 cut(s) 374
BaeGI GKGCMC 1 cut(s) 200
BalI TGGCCA 1 cut(s) 350
BarI GAAGNNNNNNTAC 2 cut(s) 117, 149
BauI CACGAG 1 cut(s) 477
BccI CCATC 2 cut(s) 466, 539
BcnI CCSGG 1 cut(s) 496
BcoDI GTCTC 2 cut(s) 70, 257
BfaI CTAG 1 cut(s) 562
BfoI RGCGCY 1 cut(s) 234
BisI GCNGC 2 cut(s) 163, 454
BlsI GCNGC 2 cut(s) 164, 455
Bme1390I CCNGG 1 cut(s) 496
Bme18I GGWCC 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 374
BmrFI CCNGG 1 cut(s) 496
BmsI GCATC 1 cut(s) 517
Bpu10I CCTNAGC 2 cut(s) 66, 192
BpuEI CTTGAG 2 cut(s) 122, 178
BpuMI CCSGG 1 cut(s) 496
BsaI GGTCTC 1 cut(s) 257
BsaWI WCCGGW 2 cut(s) 468, 521
Bsc4I CCNNNNNNNGG 2 cut(s) 449, 521
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 1 cut(s) 63
BseAI TCCGGA 1 cut(s) 468
BseGI GGATG 1 cut(s) 477
BseLI CCNNNNNNNGG 2 cut(s) 449, 521
BseMII CTCAG 2 cut(s) 80, 206
BseNI ACTGG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 200
Bsh1236I CGCG 1 cut(s) 419
BshFI GGCC 2 cut(s) 306, 350
BshTI ACCGGT 1 cut(s) 521
BsiSI CCGG 3 cut(s) 469, 496, 522
BslI CCNNNNNNNGG 2 cut(s) 449, 521
BsmAI GTCTC 2 cut(s) 70, 257
BsnI GGCC 2 cut(s) 306, 350
Bso31I GGTCTC 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 200
Bsp13I TCCGGA 1 cut(s) 468
Bsp143I GATC 4 cut(s) 71, 271, 402, 511
BspACI CCGC 5 cut(s) 162, 177, 267, 417, 453
BspANI GGCC 2 cut(s) 306, 350
BspCNI CTCAG 2 cut(s) 79, 205
BspEI TCCGGA 1 cut(s) 468
BspFNI CGCG 1 cut(s) 419
BspPI GGATC 3 cut(s) 79, 266, 506
BspTNI GGTCTC 1 cut(s) 257
BsrBI CCGCTC 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 521
BsrI ACTGG 1 cut(s) 63
BssAI RCCGGY 1 cut(s) 521
BssMI GATC 4 cut(s) 71, 271, 402, 511
BssSI CACGAG 1 cut(s) 477
Bst2BI CACGAG 1 cut(s) 477
Bst4CI ACNGT 2 cut(s) 314, 374
BstDEI CTNAG 2 cut(s) 66, 192
BstF5I GGATG 1 cut(s) 477
BstFNI CGCG 1 cut(s) 419
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 2 cut(s) 233, 301
BstKTI GATC 4 cut(s) 74, 274, 405, 514
BstMAI GTCTC 2 cut(s) 70, 257
BstMBI GATC 4 cut(s) 71, 271, 402, 511
BstMWI GCNNNNNNNGC 1 cut(s) 396
BstSCI CCNGG 1 cut(s) 494
BstSLI GKGCMC 1 cut(s) 200
BstUI CGCG 1 cut(s) 419
BsuRI GGCC 2 cut(s) 306, 350
BtgZI GCGATG 1 cut(s) 434
BtsCI GGATG 1 cut(s) 477
BtsIMutI CAGTG 1 cut(s) 56
CfoI GCGC 2 cut(s) 233, 301
Cfr10I RCCGGY 1 cut(s) 521
Cfr13I GGNCC 1 cut(s) 374
CseI GACGC 2 cut(s) 229, 260
CspAI ACCGGT 1 cut(s) 521
CviAII CATG 5 cut(s) 149, 241, 321, 352, 400
CviJI RGCY 9 cut(s) 92, 100, 191, 306, 350, 357, 476, 483, 540
CviKI_1 RGCY 9 cut(s) 92, 100, 191, 306, 350, 357, 476, 483, 540
DdeI CTNAG 2 cut(s) 66, 192
DpnI GATC 4 cut(s) 73, 273, 404, 513
DpnII GATC 4 cut(s) 71, 271, 402, 511
EaeI YGGCCR 1 cut(s) 348
Eco147I AGGCCT 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 374
Eco57I CTGAAG 1 cut(s) 119
FaeI CATG 5 cut(s) 152, 244, 324, 355, 403
FatI CATG 5 cut(s) 148, 240, 320, 351, 399
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 2 cut(s) 163, 454
FokI GGATG 1 cut(s) 484
Fsp4HI GCNGC 2 cut(s) 163, 454
FspBI CTAG 1 cut(s) 562
GlaI GCGC 2 cut(s) 232, 300
GluI GCNGC 2 cut(s) 163, 454
HaeII RGCGCY 1 cut(s) 234
HaeIII GGCC 2 cut(s) 306, 350
HapII CCGG 3 cut(s) 469, 496, 522
HgaI GACGC 2 cut(s) 229, 260
HhaI GCGC 2 cut(s) 233, 301
Hin1II CATG 5 cut(s) 152, 244, 324, 355, 403
Hin6I GCGC 2 cut(s) 231, 299
HinP1I GCGC 2 cut(s) 231, 299
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 1 cut(s) 169
HpaII CCGG 3 cut(s) 469, 496, 522
HphI GGTGA 2 cut(s) 250, 536
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 1 cut(s) 434
Hpy188III TCNNGA 4 cut(s) 24, 75, 406, 469
Hpy8I GTNNAC 1 cut(s) 247
Hpy99I CGWCG 3 cut(s) 223, 251, 254
HpyAV CCTTC 2 cut(s) 109, 143
HpyCH4III ACNGT 2 cut(s) 314, 374
HpyCH4IV ACGT 1 cut(s) 546
HpyCH4V TGCA 2 cut(s) 82, 559
HpyF10VI GCNNNNNNNGC 1 cut(s) 396
HpyF3I CTNAG 2 cut(s) 66, 192
HpySE526I ACGT 1 cut(s) 546
Hsp92II CATG 5 cut(s) 152, 244, 324, 355, 403
HspAI GCGC 2 cut(s) 231, 299
Kpn2I TCCGGA 1 cut(s) 468
Kzo9I GATC 4 cut(s) 71, 271, 402, 511
LpnPI CCDG 7 cut(s) 44, 53, 96, 179, 482, 509, 535
LweI GCATC 1 cut(s) 517
MaeI CTAG 1 cut(s) 562
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 2 cut(s) 124, 547
MalI GATC 4 cut(s) 73, 273, 404, 513
MbiI CCGCTC 1 cut(s) 162
MboI GATC 4 cut(s) 71, 271, 402, 511
MboII GAAGA 3 cut(s) 309, 341, 421
MhlI GDGCHC 1 cut(s) 200
MlsI TGGCCA 1 cut(s) 350
MluCI AATT 1 cut(s) 502
MluNI TGGCCA 1 cut(s) 350
MmeI TCCRAC 2 cut(s) 197, 271
MnlI CCTC 3 cut(s) 4, 97, 361
Mox20I TGGCCA 1 cut(s) 350
MroI TCCGGA 1 cut(s) 468
MscI TGGCCA 1 cut(s) 350
MseI TTAA 2 cut(s) 41, 183
MslI CAYNNNNRTG 2 cut(s) 398, 461
Msp20I TGGCCA 1 cut(s) 350
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 3 cut(s) 469, 496, 522
MspR9I CCNGG 1 cut(s) 496
MvnI CGCG 1 cut(s) 419
MwoI GCNNNNNNNGC 1 cut(s) 396
NciI CCSGG 1 cut(s) 496
NdeII GATC 4 cut(s) 71, 271, 402, 511
NlaIII CATG 5 cut(s) 152, 244, 324, 355, 403
PceI AGGCCT 1 cut(s) 306
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PfeI GAWTC 1 cut(s) 169
PinAI ACCGGT 1 cut(s) 521
PkrI GCNGC 2 cut(s) 164, 455
Psp1406I AACGTT 1 cut(s) 546
PspPI GGNCC 1 cut(s) 374
RseI CAYNNNNRTG 2 cut(s) 398, 461
SalI GTCGAC 1 cut(s) 245
SaqAI TTAA 2 cut(s) 41, 183
SatI GCNGC 2 cut(s) 163, 454
Sau3AI GATC 4 cut(s) 71, 271, 402, 511
Sau96I GGNCC 1 cut(s) 374
ScrFI CCNGG 1 cut(s) 496
SduI GDGCHC 1 cut(s) 200
SetI ASST 7 cut(s) 126, 135, 158, 189, 340, 359, 549
SfaNI GCATC 1 cut(s) 517
SinI GGWCC 1 cut(s) 374
SmiMI CAYNNNNRTG 2 cut(s) 398, 461
SmlI CTYRAG 2 cut(s) 101, 157
SmoI CTYRAG 2 cut(s) 101, 157
Sse9I AATT 1 cut(s) 502
SseBI AGGCCT 1 cut(s) 306
SsiI CCGC 5 cut(s) 162, 177, 267, 417, 453
SspMI CTAG 1 cut(s) 562
StuI AGGCCT 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 494
TaaI ACNGT 2 cut(s) 314, 374
TaiI ACGT 1 cut(s) 549
TaqI TCGA 3 cut(s) 74, 246, 366
TasI AATT 1 cut(s) 502
TauI GCSGC 2 cut(s) 165, 456
TfiI GAWTC 1 cut(s) 169
Tru1I TTAA 2 cut(s) 41, 183
Tru9I TTAA 2 cut(s) 41, 183
TscAI CASTG 1 cut(s) 63
TspDTI ATGAA 3 cut(s) 229, 309, 437
TspRI CASTG 1 cut(s) 63
VpaK11BI GGWCC 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.