MD05G1117500.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003188
Physical Location & Seq
Forward (+)
23850371 .. 23850949
579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1117500.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGCAAACGCAGCCGTGACTGTGATGCTCATGCTCATCATGGCCGTCGTAGCTGAAACCACCGAAGGAGCTCAGAGCAGCTCCACACAACCCAAGTGGGCCAAGAAGCTCAAGACAAGGAAGAAAACGACGTCGGAGACAGTGACCAACCTCCAATTCTACTTCCACGACACAGTCAGTGGGAAGAACCCAAGTGCCGTCCGTGTAGCGCAAGCCCCAGAGACCGAGAAGTCCCTCACCCTTTTCGGAGCTCTGCTCATGGCAGACGACCCGCTAACCGAAACGCCTGACCCGAAGTCCAAGCTCGTGGGCCGGGCGCAGGGGATGTACGGGTCATCCGGGCAGCAAGAGTTTGGGCTTACTATGGCCATGTGCTTCGCGTTCTGTGATGGCATGTACAATGGGAGCTCGATCAGTATTTTTGGTAAGAACTCGGCTATGAATCCGGTTCGGGAGATGCCGGTAGTGGCCGGAACCGGCGTTTTCCGGTTGGCGAGGGGTTATGCAATTGCCAAGACGCATTGGTTTAATACTATGGGTGATGCAATTGTTGGATACAATGTCACTCTAATTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

193

Amino Acids

20.69

Weight (kDa)

9.1

Isoelectric Point (pI)

27.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 48 - 190 1.9e-53 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 229
AatII GACGTC 1 cut(s) 134
AccII CGCG 1 cut(s) 380
AciI CCGC 1 cut(s) 272
AcoI YGGCCR 3 cut(s) 42, 366, 468
AcyI GRCGYC 1 cut(s) 131
AfaI GTAC 2 cut(s) 329, 398
AfiI CCNNNNNNNGG 1 cut(s) 319
AhdI GACNNNNNGTC 1 cut(s) 295
AjuI GAANNNNNNNTTGG 2 cut(s) 140, 172
AluBI AGCT 7 cut(s) 53, 71, 81, 109, 251, 304, 408
AluI AGCT 7 cut(s) 53, 71, 81, 109, 251, 304, 408
Alw21I GWGCWC 3 cut(s) 73, 253, 410
Alw26I GTCTC 2 cut(s) 131, 215
AoxI GGCC 5 cut(s) 42, 99, 310, 366, 468
ApeKI GCWGC 3 cut(s) 11, 78, 343
AspLEI GCGC 2 cut(s) 211, 319
AspS9I GGNCC 2 cut(s) 99, 310
AsuC2I CCSGG 2 cut(s) 314, 340
AsuHPI GGTGA 2 cut(s) 229, 551
BalI TGGCCA 1 cut(s) 368
BanII GRGCYC 3 cut(s) 73, 253, 410
BauI CACGAG 1 cut(s) 305
Bbv12I GWGCWC 3 cut(s) 73, 253, 410
BbvI GCAGC 3 cut(s) 23, 90, 355
BccI CCATC 1 cut(s) 383
BceAI ACGGC 2 cut(s) 29, 182
BciVI GTATCC 1 cut(s) 548
BcnI CCSGG 2 cut(s) 314, 340
BcoDI GTCTC 2 cut(s) 131, 215
BfaI CTAG 1 cut(s) 577
BfuI GTATCC 1 cut(s) 548
BisI GCNGC 3 cut(s) 12, 79, 344
BlsI GCNGC 3 cut(s) 13, 80, 345
Bme1390I CCNGG 2 cut(s) 314, 340
BmeRI GACNNNNNGTC 1 cut(s) 295
BmgT120I GGNCC 2 cut(s) 99, 310
BmiI GGNNCC 1 cut(s) 475
BmrFI CCNGG 2 cut(s) 314, 340
BmsI GCATC 3 cut(s) 15, 447, 532
BplI GAGNNNNNCTC 2 cut(s) 240, 272
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 2 cut(s) 314, 340
BsaHI GRCGYC 1 cut(s) 131
BsaI GGTCTC 1 cut(s) 215
BsaWI WCCGGW 2 cut(s) 445, 486
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse118I RCCGGY 2 cut(s) 460, 476
BseGI GGATG 2 cut(s) 330, 335
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMII CTCAG 1 cut(s) 86
BseXI GCAGC 3 cut(s) 23, 90, 355
Bsh1236I CGCG 1 cut(s) 380
BshFI GGCC 5 cut(s) 44, 101, 312, 368, 470
BsiHKAI GWGCWC 3 cut(s) 73, 253, 410
BsiSI CCGG 7 cut(s) 313, 339, 446, 461, 471, 477, 487
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 2 cut(s) 131, 215
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 5 cut(s) 44, 101, 312, 368, 470
Bso31I GGTCTC 1 cut(s) 215
Bsp1286I GDGCHC 3 cut(s) 73, 253, 410
Bsp1407I TGTACA 1 cut(s) 396
Bsp143I GATC 1 cut(s) 411
BspACI CCGC 1 cut(s) 272
BspANI GGCC 5 cut(s) 44, 101, 312, 368, 470
BspCNI CTCAG 1 cut(s) 85
BspFNI CGCG 1 cut(s) 380
BspLI GGNNCC 1 cut(s) 475
BspTNI GGTCTC 1 cut(s) 215
BsrFI RCCGGY 2 cut(s) 460, 476
BsrGI TGTACA 1 cut(s) 396
BssAI RCCGGY 2 cut(s) 460, 476
BssMI GATC 1 cut(s) 411
BssNI GRCGYC 1 cut(s) 131
BssSI CACGAG 1 cut(s) 305
Bst2BI CACGAG 1 cut(s) 305
Bst4CI ACNGT 3 cut(s) 22, 142, 175
BstACI GRCGYC 1 cut(s) 131
BstAUI TGTACA 1 cut(s) 396
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 1 cut(s) 72
BstF5I GGATG 2 cut(s) 330, 335
BstFNI CGCG 1 cut(s) 380
BstHHI GCGC 2 cut(s) 211, 319
BstKTI GATC 1 cut(s) 414
BstMAI GTCTC 2 cut(s) 131, 215
BstMBI GATC 1 cut(s) 411
BstMWI GCNNNNNNNGC 2 cut(s) 11, 50
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 2 cut(s) 312, 338
BstUI CGCG 1 cut(s) 380
BstV1I GCAGC 3 cut(s) 23, 90, 355
BstXI CCANNNNNNTGG 1 cut(s) 307
BsuI GTATCC 1 cut(s) 548
BsuRI GGCC 5 cut(s) 44, 101, 312, 368, 470
BtsCI GGATG 2 cut(s) 330, 335
BtsIMutI CAGTG 2 cut(s) 147, 184
Cac8I GCNNGC 1 cut(s) 213
CfoI GCGC 2 cut(s) 211, 319
Cfr10I RCCGGY 2 cut(s) 460, 476
Cfr13I GGNCC 2 cut(s) 99, 310
CseI GACGC 1 cut(s) 526
Csp6I GTAC 2 cut(s) 328, 397
CspCI CAANNNNNGTGG 2 cut(s) 77, 112
CviAII CATG 5 cut(s) 31, 40, 259, 370, 394
CviQI GTAC 2 cut(s) 328, 397
DdeI CTNAG 1 cut(s) 72
DpnI GATC 1 cut(s) 413
DpnII GATC 1 cut(s) 411
DrdI GACNNNNNNGTC 1 cut(s) 229
DriI GACNNNNNGTC 1 cut(s) 295
DseDI GACNNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 3 cut(s) 42, 366, 468
Eam1105I GACNNNNNGTC 1 cut(s) 295
Ecl136II GAGCTC 3 cut(s) 71, 251, 408
Eco24I GRGCYC 3 cut(s) 73, 253, 410
Eco31I GGTCTC 1 cut(s) 215
Eco53kI GAGCTC 3 cut(s) 71, 251, 408
EcoICRI GAGCTC 3 cut(s) 71, 251, 408
EcoT38I GRGCYC 3 cut(s) 73, 253, 410
FaeI CATG 5 cut(s) 34, 43, 262, 373, 397
FaiI YATR 9 cut(s) 32, 41, 260, 365, 371, 395, 440, 504, 536
FaqI GGGAC 1 cut(s) 217
FatI CATG 5 cut(s) 30, 39, 258, 369, 393
FauI CCCGC 1 cut(s) 279
Fnu4HI GCNGC 3 cut(s) 12, 79, 344
FokI GGATG 2 cut(s) 322, 337
FriOI GRGCYC 3 cut(s) 73, 253, 410
Fsp4HI GCNGC 3 cut(s) 12, 79, 344
FspBI CTAG 1 cut(s) 577
GlaI GCGC 2 cut(s) 210, 318
GluI GCNGC 3 cut(s) 12, 79, 344
HaeIII GGCC 5 cut(s) 44, 101, 312, 368, 470
HapII CCGG 7 cut(s) 313, 339, 446, 461, 471, 477, 487
HgaI GACGC 1 cut(s) 526
HhaI GCGC 2 cut(s) 211, 319
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 5 cut(s) 34, 43, 262, 373, 397
Hin6I GCGC 2 cut(s) 209, 317
HinP1I GCGC 2 cut(s) 209, 317
HinfI GANTC 1 cut(s) 442
HpaII CCGG 7 cut(s) 313, 339, 446, 461, 471, 477, 487
HphI GGTGA 2 cut(s) 229, 551
Hpy188I TCNGA 3 cut(s) 75, 136, 248
Hpy188III TCNNGA 2 cut(s) 112, 452
Hpy99I CGWCG 3 cut(s) 50, 133, 136
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 3 cut(s) 22, 142, 175
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 2 cut(s) 506, 545
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 50
HpyF3I CTNAG 1 cut(s) 72
HpySE526I ACGT 1 cut(s) 131
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 5 cut(s) 34, 43, 262, 373, 397
HspAI GCGC 2 cut(s) 209, 317
Kzo9I GATC 1 cut(s) 411
LmnI GCTCC 4 cut(s) 68, 86, 248, 405
Lsp1109I GCAGC 3 cut(s) 23, 90, 355
LweI GCATC 3 cut(s) 15, 447, 532
MaeI CTAG 1 cut(s) 577
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 3 cut(s) 16, 142, 562
MalI GATC 1 cut(s) 413
MboI GATC 1 cut(s) 411
MboII GAAGA 2 cut(s) 133, 196
MfeI CAATTG 2 cut(s) 507, 546
MhlI GDGCHC 3 cut(s) 73, 253, 410
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 4 cut(s) 155, 507, 546, 570
MluNI TGGCCA 1 cut(s) 368
MmeI TCCRAC 2 cut(s) 114, 532
MnlI CCTC 3 cut(s) 161, 245, 489
Mox20I TGGCCA 1 cut(s) 368
MscI TGGCCA 1 cut(s) 368
MseI TTAA 1 cut(s) 528
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 7 cut(s) 313, 339, 446, 461, 471, 477, 487
MspR9I CCNGG 2 cut(s) 314, 340
MunI CAATTG 2 cut(s) 507, 546
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 2 cut(s) 11, 50
NciI CCSGG 2 cut(s) 314, 340
NdeII GATC 1 cut(s) 411
NlaIII CATG 5 cut(s) 34, 43, 262, 373, 397
NlaIV GGNNCC 1 cut(s) 475
NmeAIII GCCGAG 1 cut(s) 413
NmuCI GTSAC 3 cut(s) 16, 142, 562
NspI RCATGY 1 cut(s) 397
PcsI WCGNNNNNNNCGW 1 cut(s) 290
PfeI GAWTC 1 cut(s) 442
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 3 cut(s) 13, 80, 345
Psp124BI GAGCTC 3 cut(s) 73, 253, 410
PspN4I GGNNCC 1 cut(s) 475
PspPI GGNCC 2 cut(s) 99, 310
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 2 cut(s) 329, 398
RsaNI GTAC 2 cut(s) 328, 397
SacI GAGCTC 3 cut(s) 73, 253, 410
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 3 cut(s) 12, 79, 344
Sau3AI GATC 1 cut(s) 411
Sau96I GGNCC 2 cut(s) 99, 310
ScrFI CCNGG 2 cut(s) 314, 340
SduI GDGCHC 3 cut(s) 73, 253, 410
SetI ASST 9 cut(s) 55, 73, 83, 111, 134, 153, 253, 306, 410
SfaNI GCATC 3 cut(s) 15, 447, 532
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 4 cut(s) 155, 507, 546, 570
SsiI CCGC 1 cut(s) 272
SspMI CTAG 1 cut(s) 577
SstI GAGCTC 3 cut(s) 73, 253, 410
StyD4I CCNGG 2 cut(s) 312, 338
TaaI ACNGT 3 cut(s) 22, 142, 175
TaiI ACGT 1 cut(s) 134
TaqI TCGA 1 cut(s) 410
TaqII GACCGA 1 cut(s) 239
TasI AATT 4 cut(s) 155, 507, 546, 570
TatI WGTACW 1 cut(s) 396
TfiI GAWTC 1 cut(s) 442
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 2 cut(s) 147, 184
TseFI GTSAC 3 cut(s) 16, 142, 562
TseI GCWGC 3 cut(s) 11, 78, 343
Tsp45I GTSAC 3 cut(s) 16, 142, 562
TspDTI ATGAA 1 cut(s) 455
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 2 cut(s) 147, 184
Tth111I GACNNNGTC 1 cut(s) 173
XceI RCATGY 1 cut(s) 397
XspI CTAG 1 cut(s) 577
ZraI GACGTC 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.