Rh6AG025200

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003188
Physical Location & Seq
Reverse (-)
2913989 .. 2915289
1301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG025200.1

Sequence Viewer

Length: 561 bp
ATGGCAAACACAACTCTACATCTGGTGCTGATGGTGTTCATCTCCTTGGTGGTGTCCTTCACTGAAGCGAAGTGGGCTGAGTCCGTGGAGGCAACAAAGGAAGCGACGCCATCGGAGACAGTAACCAACCTCCAGTTCTACTTCCACGACACAGTCAGTGGGAAGAACCCTAGTGCCGTACGTGTGGCTCAAGCTTCCGACACCGAAAAGTCTCCGACACTTTTCGGGGCTTTACTCATGGCTGATGACCCGCTCACCGAGACTCCCGACCCCAATTCCAAGCTCGTAGGACGGGCACAAGGGCTATACGGGTCATCCTGCCAGCAGGAACTTGGCCTGATTATGGCCATGAGCTTTGCGTTCTCGGACGGTATGTACAATGGTAGCTCCATTAGTATTTTTGGAAAGAACCCGATCACGAACCCAATTCGGGAGTTGCCGGTTGTGGGTGGAACTGGGGTTTTCCGGTTGGCTCGTGGCTATGCAATTGCCCATACGCATTGGGTAAATGCCACAGCTGGCGATGCAGTTGTTGGATACAATGTCACTGTAGTTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

186

Amino Acids

19.86

Weight (kDa)

5.43

Isoelectric Point (pI)

22.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 41 - 184 3.7e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 253
AciI CCGC 1 cut(s) 251
AcoI YGGCCR 1 cut(s) 345
AcuI CTGAAG 1 cut(s) 84
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 2 cut(s) 180, 377
AfiI CCNNNNNNNGG 3 cut(s) 343, 430, 446
AflIII ACRYGT 1 cut(s) 181
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 5 cut(s) 194, 283, 354, 387, 518
AluI AGCT 5 cut(s) 194, 283, 354, 387, 518
Alw26I GTCTC 3 cut(s) 110, 216, 254
AoxI GGCC 2 cut(s) 334, 345
AsuHPI GGTGA 1 cut(s) 247
BaeGI GKGCMC 1 cut(s) 298
BalI TGGCCA 1 cut(s) 347
BauI CACGAG 1 cut(s) 474
BccI CCATC 2 cut(s) 25, 118
BceAI ACGGC 1 cut(s) 161
BciVI GTATCC 1 cut(s) 530
BcoDI GTCTC 3 cut(s) 110, 216, 254
BfaI CTAG 2 cut(s) 171, 559
BfmI CTRYAG 1 cut(s) 549
BfuI GTATCC 1 cut(s) 530
BmrI ACTGGG 1 cut(s) 465
BmsI GCATC 1 cut(s) 514
BmuI ACTGGG 1 cut(s) 465
BpmI CTGGAG 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 174
BsaAI YACGTR 1 cut(s) 182
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 2 cut(s) 45, 84
BsaWI WCCGGW 1 cut(s) 465
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 430, 446
Bse118I RCCGGY 1 cut(s) 439
Bse1I ACTGG 2 cut(s) 133, 460
BseDI CCNNGG 2 cut(s) 45, 84
BseGI GGATG 1 cut(s) 314
BseLI CCNNNNNNNGG 3 cut(s) 343, 430, 446
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 2 cut(s) 133, 460
BseSI GKGCMC 1 cut(s) 298
BshFI GGCC 2 cut(s) 336, 347
BsiSI CCGG 2 cut(s) 440, 466
BsiWI CGTACG 1 cut(s) 178
BslI CCNNNNNNNGG 3 cut(s) 343, 430, 446
BsmAI GTCTC 3 cut(s) 110, 216, 254
BsnI GGCC 2 cut(s) 336, 347
Bsp1286I GDGCHC 1 cut(s) 298
Bsp1407I TGTACA 1 cut(s) 375
Bsp143I GATC 1 cut(s) 414
BspACI CCGC 1 cut(s) 251
BspANI GGCC 2 cut(s) 336, 347
BspCNI CTCAG 1 cut(s) 70
BsrBI CCGCTC 1 cut(s) 253
BsrFI RCCGGY 1 cut(s) 439
BsrGI TGTACA 1 cut(s) 375
BsrI ACTGG 2 cut(s) 133, 460
BssAI RCCGGY 1 cut(s) 439
BssECI CCNNGG 2 cut(s) 45, 84
BssMI GATC 1 cut(s) 414
BssNI GRCGYC 1 cut(s) 107
BssSI CACGAG 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 45
Bst2BI CACGAG 1 cut(s) 474
Bst4CI ACNGT 4 cut(s) 121, 154, 371, 550
BstACI GRCGYC 1 cut(s) 107
BstAUI TGTACA 1 cut(s) 375
BstBAI YACGTR 1 cut(s) 182
BstC8I GCNNGC 2 cut(s) 323, 520
BstDEI CTNAG 1 cut(s) 78
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 1 cut(s) 314
BstKTI GATC 1 cut(s) 417
BstMAI GTCTC 3 cut(s) 110, 216, 254
BstMBI GATC 1 cut(s) 414
BstMWI GCNNNNNNNGC 2 cut(s) 74, 524
BstSFI CTRYAG 1 cut(s) 549
BstSLI GKGCMC 1 cut(s) 298
BsuI GTATCC 1 cut(s) 530
BsuRI GGCC 2 cut(s) 336, 347
BtgI CCRYGG 1 cut(s) 84
BtgZI GCGATG 1 cut(s) 537
BtsCI GGATG 1 cut(s) 314
BtsIMutI CAGTG 3 cut(s) 60, 163, 546
Cac8I GCNNGC 2 cut(s) 323, 520
Cfr10I RCCGGY 1 cut(s) 439
CseI GACGC 1 cut(s) 115
Csp6I GTAC 2 cut(s) 179, 376
CviAII CATG 2 cut(s) 238, 349
CviQI GTAC 2 cut(s) 179, 376
DdeI CTNAG 1 cut(s) 78
DpnI GATC 1 cut(s) 416
DpnII GATC 1 cut(s) 414
EaeI YGGCCR 1 cut(s) 345
Eco130I CCWWGG 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 2 cut(s) 241, 352
FaiI YATR 7 cut(s) 239, 307, 344, 350, 374, 483, 495
FatI CATG 2 cut(s) 237, 348
FauI CCCGC 1 cut(s) 258
FokI GGATG 1 cut(s) 301
FspBI CTAG 2 cut(s) 171, 559
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 2 cut(s) 336, 347
HapII CCGG 2 cut(s) 440, 466
HgaI GACGC 1 cut(s) 115
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 2 cut(s) 241, 352
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 2 cut(s) 80, 262
HpaII CCGG 2 cut(s) 440, 466
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 556
Hpy188I TCNGA 4 cut(s) 115, 199, 216, 367
Hpy188III TCNNGA 3 cut(s) 266, 418, 431
Hpy8I GTNNAC 1 cut(s) 556
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 4 cut(s) 121, 154, 371, 550
HpyCH4IV ACGT 1 cut(s) 181
HpyCH4V TGCA 2 cut(s) 485, 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 524
HpyF3I CTNAG 1 cut(s) 78
HpySE526I ACGT 1 cut(s) 181
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 2 cut(s) 241, 352
Kzo9I GATC 1 cut(s) 414
LmnI GCTCC 1 cut(s) 392
LweI GCATC 1 cut(s) 514
MaeI CTAG 2 cut(s) 171, 559
MaeII ACGT 1 cut(s) 181
MaeIII GTNAC 2 cut(s) 121, 544
MalI GATC 1 cut(s) 416
MbiI CCGCTC 1 cut(s) 253
MboI GATC 1 cut(s) 414
MboII GAAGA 1 cut(s) 175
MfeI CAATTG 1 cut(s) 486
MhlI GDGCHC 1 cut(s) 298
MlsI TGGCCA 1 cut(s) 347
MluCI AATT 3 cut(s) 274, 426, 486
MluNI TGGCCA 1 cut(s) 347
MlyI GAGTC 2 cut(s) 89, 256
MmeI TCCRAC 3 cut(s) 222, 239, 514
MnlI CCTC 2 cut(s) 82, 140
Mox20I TGGCCA 1 cut(s) 347
MscI TGGCCA 1 cut(s) 347
Msp20I TGGCCA 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 2 cut(s) 440, 466
MunI CAATTG 1 cut(s) 486
MwoI GCNNNNNNNGC 2 cut(s) 74, 524
NdeII GATC 1 cut(s) 414
NlaIII CATG 2 cut(s) 241, 352
NmuCI GTSAC 1 cut(s) 544
Pfl23II CGTACG 1 cut(s) 178
PflFI GACNNNGTC 1 cut(s) 152
PleI GAGTC 2 cut(s) 88, 256
PpsI GAGTC 2 cut(s) 88, 256
Ppu21I YACGTR 1 cut(s) 182
PspLI CGTACG 1 cut(s) 178
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 2 cut(s) 180, 377
RsaNI GTAC 2 cut(s) 179, 376
Sau3AI GATC 1 cut(s) 414
SchI GAGTC 2 cut(s) 89, 256
SduI GDGCHC 1 cut(s) 298
SetI ASST 7 cut(s) 132, 184, 196, 285, 356, 389, 520
SfaNI GCATC 1 cut(s) 514
SfcI CTRYAG 1 cut(s) 549
SmlI CTYRAG 1 cut(s) 189
SmoI CTYRAG 1 cut(s) 189
Sse9I AATT 3 cut(s) 274, 426, 486
SsiI CCGC 1 cut(s) 251
SspMI CTAG 2 cut(s) 171, 559
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 4 cut(s) 121, 154, 371, 550
TaiI ACGT 1 cut(s) 184
TasI AATT 3 cut(s) 274, 426, 486
TatI WGTACW 1 cut(s) 375
TscAI CASTG 3 cut(s) 67, 163, 553
TseFI GTSAC 1 cut(s) 544
Tsp45I GTSAC 1 cut(s) 544
TspDTI ATGAA 1 cut(s) 28
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 3 cut(s) 67, 163, 553
Tth111I GACNNNGTC 1 cut(s) 152
XcmI CCANNNNNNNNNTGG 1 cut(s) 329
XspI CTAG 2 cut(s) 171, 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.