FvH4_2g01681

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003188
Physical Location & Seq
Reverse (-)
1504720 .. 1505713
994 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01681.t1

Sequence Viewer

Length: 564 bp
ATGGCAAACACATCTCTGCCTCTGGTGTTGACGGTGTTCATCTCCTTGGTGGTGTCCTTCACGGAGGCGAAATGGGCTGAGTCTGCGGCGGCGAAAAAGGAAGCAACACCGTCTGAGACTACAGTAACCAACCTCCAGTTCTACTTCCACGACACAATCAGTGGGAAGAACCCTAGCGCCGTACGTGTGGCGCAAGCCTCCGACACCGAAAAGTCCCCGACGCTTTTCGGAGCCTTACTCATGGCCGACGACCCACTCACCGAGACTCCCGACCCCAATTCCAAGCTGCTCGGCCGGGCACAAGGGATGTACGGGTCCTCTGGTCAGCAGGAGTTTGGGTTGATTATGGCCATGAGCTTTGCTTTCACAGAAGGTACGTACAATGGTAGCTCCATTAGTATTTTTGGAAAAAATCCGATCACGAATCCGATTAGGGAGTTGCCGGTTGTGGGTGGAACTGGGATTTTCCGGTCGGCTCGTGGATATGCGATTGCTCATACGCATTGGTTCAATAATGACAGTGGCGATGCGATTGTAGGATACAATCTAACTGTAGTTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

188

Amino Acids

19.98

Weight (kDa)

5.49

Isoelectric Point (pI)

26.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 42 - 185 1.9e-55 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 86, 89
AcoI YGGCCR 3 cut(s) 243, 292, 348
AfaI GTAC 4 cut(s) 183, 311, 376, 380
AfiI CCNNNNNNNGG 1 cut(s) 449
AflIII ACRYGT 1 cut(s) 184
AgsI TTSAA 1 cut(s) 511
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 3 cut(s) 286, 357, 390
AluI AGCT 3 cut(s) 286, 357, 390
Alw26I GTCTC 2 cut(s) 110, 257
AoxI GGCC 3 cut(s) 243, 292, 348
ApeKI GCWGC 1 cut(s) 286
AspLEI GCGC 2 cut(s) 179, 193
AspS9I GGNCC 1 cut(s) 315
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 250
AvaII GGWCC 1 cut(s) 315
BaeGI GKGCMC 1 cut(s) 301
BalI TGGCCA 1 cut(s) 350
BauI CACGAG 1 cut(s) 477
BbvI GCAGC 1 cut(s) 273
BceAI ACGGC 1 cut(s) 164
BciVI GTATCC 1 cut(s) 533
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 2 cut(s) 110, 257
BfaI CTAG 2 cut(s) 174, 562
BfmI CTRYAG 2 cut(s) 120, 552
BfoI RGCGCY 1 cut(s) 180
BfuI GTATCC 1 cut(s) 533
BisI GCNGC 3 cut(s) 87, 90, 287
BlsI GCNGC 3 cut(s) 88, 91, 288
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 1 cut(s) 315
BmgT120I GGNCC 1 cut(s) 315
BmiI GGNNCC 2 cut(s) 232, 316
BmrFI CCNGG 1 cut(s) 296
BmrI ACTGGG 1 cut(s) 468
BmsI GCATC 1 cut(s) 517
BmuI ACTGGG 1 cut(s) 468
BplI GAGNNNNNCTC 2 cut(s) 222, 254
BpmI CTGGAG 1 cut(s) 119
BpuMI CCSGG 1 cut(s) 296
BsaAI YACGTR 2 cut(s) 185, 378
BsaJI CCNNGG 1 cut(s) 45
BsaWI WCCGGW 1 cut(s) 468
BsaXI ACNNNNNCTCC 4 cut(s) 250, 280, 323, 353
Bsc4I CCNNNNNNNGG 1 cut(s) 449
Bse118I RCCGGY 1 cut(s) 442
Bse1I ACTGG 2 cut(s) 136, 463
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 1 cut(s) 312
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMII CTCAG 2 cut(s) 69, 105
BseNI ACTGG 2 cut(s) 136, 463
BseSI GKGCMC 1 cut(s) 301
BseX3I CGGCCG 1 cut(s) 292
BseXI GCAGC 1 cut(s) 273
Bsh1285I CGRYCG 2 cut(s) 295, 473
BshFI GGCC 3 cut(s) 245, 294, 350
BsiEI CGRYCG 2 cut(s) 295, 473
BsiSI CCGG 3 cut(s) 295, 443, 469
BsiWI CGTACG 1 cut(s) 181
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 2 cut(s) 110, 257
BsmFI GGGAC 1 cut(s) 199
BsnI GGCC 3 cut(s) 245, 294, 350
Bsp1286I GDGCHC 1 cut(s) 301
Bsp143I GATC 1 cut(s) 417
BspACI CCGC 2 cut(s) 86, 89
BspANI GGCC 3 cut(s) 245, 294, 350
BspCNI CTCAG 2 cut(s) 70, 106
BspLI GGNNCC 2 cut(s) 232, 316
BsrFI RCCGGY 1 cut(s) 442
BsrI ACTGG 2 cut(s) 136, 463
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 1 cut(s) 417
BssSI CACGAG 1 cut(s) 477
BssT1I CCWWGG 1 cut(s) 45
Bst2BI CACGAG 1 cut(s) 477
Bst4CI ACNGT 5 cut(s) 34, 111, 124, 521, 553
BstBAI YACGTR 2 cut(s) 185, 378
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 2 cut(s) 78, 114
BstF5I GGATG 1 cut(s) 312
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 2 cut(s) 179, 193
BstKTI GATC 1 cut(s) 420
BstMAI GTCTC 2 cut(s) 110, 257
BstMBI GATC 1 cut(s) 417
BstMCI CGRYCG 2 cut(s) 295, 473
BstMWI GCNNNNNNNGC 2 cut(s) 74, 83
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 2 cut(s) 120, 552
BstSLI GKGCMC 1 cut(s) 301
BstSNI TACGTA 1 cut(s) 378
BstV1I GCAGC 1 cut(s) 273
BstZI CGGCCG 1 cut(s) 292
BsuI GTATCC 1 cut(s) 533
BsuRI GGCC 3 cut(s) 245, 294, 350
BtgZI GCGATG 1 cut(s) 540
BtsCI GGATG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 166, 526
Cac8I GCNNGC 1 cut(s) 195
CfoI GCGC 2 cut(s) 179, 193
Cfr10I RCCGGY 1 cut(s) 442
Cfr13I GGNCC 1 cut(s) 315
CseI GACGC 1 cut(s) 229
Csp6I GTAC 4 cut(s) 182, 310, 375, 379
CviAII CATG 2 cut(s) 241, 352
CviQI GTAC 4 cut(s) 182, 310, 375, 379
DdeI CTNAG 2 cut(s) 78, 114
DpnI GATC 1 cut(s) 419
DpnII GATC 1 cut(s) 417
EaeI YGGCCR 3 cut(s) 243, 292, 348
EagI CGGCCG 1 cut(s) 292
EclXI CGGCCG 1 cut(s) 292
Eco105I TACGTA 1 cut(s) 378
Eco130I CCWWGG 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 315
Eco52I CGGCCG 1 cut(s) 292
EcoO109I RGGNCCY 1 cut(s) 315
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 2 cut(s) 244, 355
FaiI YATR 5 cut(s) 242, 347, 353, 486, 498
FaqI GGGAC 1 cut(s) 199
FatI CATG 2 cut(s) 240, 351
Fnu4HI GCNGC 3 cut(s) 87, 90, 287
FokI GGATG 1 cut(s) 319
Fsp4HI GCNGC 3 cut(s) 87, 90, 287
FspBI CTAG 2 cut(s) 174, 562
GlaI GCGC 2 cut(s) 178, 192
GluI GCNGC 3 cut(s) 87, 90, 287
GsuI CTGGAG 1 cut(s) 119
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 3 cut(s) 245, 294, 350
HapII CCGG 3 cut(s) 295, 443, 469
HgaI GACGC 1 cut(s) 229
HhaI GCGC 2 cut(s) 179, 193
Hin1II CATG 2 cut(s) 244, 355
Hin6I GCGC 2 cut(s) 177, 191
HinP1I GCGC 2 cut(s) 177, 191
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 3 cut(s) 80, 265, 424
HpaII CCGG 3 cut(s) 295, 443, 469
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 2 cut(s) 30, 559
Hpy188I TCNGA 5 cut(s) 115, 202, 230, 417, 429
Hpy188III TCNNGA 2 cut(s) 269, 421
Hpy8I GTNNAC 2 cut(s) 30, 559
Hpy99I CGWCG 2 cut(s) 223, 251
HpyAV CCTTC 2 cut(s) 67, 365
HpyCH4III ACNGT 5 cut(s) 34, 111, 124, 521, 553
HpyCH4IV ACGT 2 cut(s) 184, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 83
HpyF3I CTNAG 2 cut(s) 78, 114
HpySE526I ACGT 2 cut(s) 184, 377
Hsp92II CATG 2 cut(s) 244, 355
HspAI GCGC 2 cut(s) 177, 191
Kzo9I GATC 1 cut(s) 417
LmnI GCTCC 2 cut(s) 230, 395
LpnPI CCDG 8 cut(s) 8, 149, 306, 308, 314, 444, 456, 482
Lsp1109I GCAGC 1 cut(s) 273
LweI GCATC 1 cut(s) 517
MaeI CTAG 2 cut(s) 174, 562
MaeII ACGT 2 cut(s) 184, 377
MaeIII GTNAC 1 cut(s) 124
MalI GATC 1 cut(s) 419
MboI GATC 1 cut(s) 417
MboII GAAGA 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 301
MlsI TGGCCA 1 cut(s) 350
MluCI AATT 1 cut(s) 277
MluNI TGGCCA 1 cut(s) 350
MlyI GAGTC 2 cut(s) 89, 259
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 5 cut(s) 30, 58, 143, 208, 328
Mox20I TGGCCA 1 cut(s) 350
MscI TGGCCA 1 cut(s) 350
Msp20I TGGCCA 1 cut(s) 350
MspI CCGG 3 cut(s) 295, 443, 469
MspR9I CCNGG 1 cut(s) 296
MwoI GCNNNNNNNGC 2 cut(s) 74, 83
NciI CCSGG 1 cut(s) 296
NdeII GATC 1 cut(s) 417
NlaIII CATG 2 cut(s) 244, 355
NlaIV GGNNCC 2 cut(s) 232, 316
NmeAIII GCCGAG 1 cut(s) 270
PfeI GAWTC 1 cut(s) 424
Pfl23II CGTACG 1 cut(s) 181
PkrI GCNGC 3 cut(s) 88, 91, 288
PleI GAGTC 2 cut(s) 88, 259
PpsI GAGTC 2 cut(s) 88, 259
Ppu21I YACGTR 2 cut(s) 185, 378
PpuMI RGGWCCY 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 315
PspLI CGTACG 1 cut(s) 181
PspN4I GGNNCC 2 cut(s) 232, 316
PspPI GGNCC 1 cut(s) 315
PspPPI RGGWCCY 1 cut(s) 315
RsaI GTAC 4 cut(s) 183, 311, 376, 380
RsaNI GTAC 4 cut(s) 182, 310, 375, 379
SatI GCNGC 3 cut(s) 87, 90, 287
Sau3AI GATC 1 cut(s) 417
Sau96I GGNCC 1 cut(s) 315
SchI GAGTC 2 cut(s) 89, 259
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 1 cut(s) 301
SetI ASST 7 cut(s) 135, 187, 288, 359, 376, 380, 392
SfaNI GCATC 1 cut(s) 517
SfcI CTRYAG 2 cut(s) 120, 552
SinI GGWCC 1 cut(s) 315
SnaBI TACGTA 1 cut(s) 378
Sse9I AATT 1 cut(s) 277
SsiI CCGC 2 cut(s) 86, 89
SspMI CTAG 2 cut(s) 174, 562
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 5 cut(s) 34, 111, 124, 521, 553
TaiI ACGT 2 cut(s) 187, 380
TasI AATT 1 cut(s) 277
TauI GCSGC 2 cut(s) 89, 92
TfiI GAWTC 1 cut(s) 424
TscAI CASTG 2 cut(s) 166, 526
TseI GCWGC 1 cut(s) 286
TspDTI ATGAA 1 cut(s) 28
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 2 cut(s) 166, 526
VpaK11BI GGWCC 1 cut(s) 315
XspI CTAG 2 cut(s) 174, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.