Rh6CG018500

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0003188
Physical Location & Seq
Reverse (-)
1843742 .. 1844424
683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG018500.1

Sequence Viewer

Length: 681 bp
ATGGTGCTAGCTAGTTCAACTGCATTCAATCCCCGCCCATTATTTAAGACTCAAAATCAGCCCAGACACCGACATCTCCTCTTTCTCTGGAATAAACACGACAGAAATTGGTCAAAAATGGCAAACACAGCTCTGCCGCTAATGATGTTAATGATCACCTCCTTGCTGATGGCCTTGACGGAAGCTGAGTGGGCAGAGTCAGTTGAAGCAAAAAAGGAGGCCACGCCTTTGGAGACCGTAACCAACCTCCAGTTCTACTTCCACGACACAGTCAGTGGGAAGAACCCCAGTGCCGTACGTGTGGCACAAGCCGCCAACACCGACAAGTCCCCAACCCTCTTTGGGGCTCTGCTCATGGCCGATGACCCGCTTACTGAGACGCCGGACCCTAATTCCAAGCTCGTGGGACGGGCGCAAGGGCTGTATGGGTCATCCGGCCAGCAGGAATTTGGCCTCATTATGGCCATGAGCTTTGCGTTCACGGATGGTGCGTTCAATGGTAGCTCCGTTAGTATTTTTGGTAAAAACCCAGCTCTACACCCGGTTCGGGAGTTGCCGGTTGTGGGTGGAACTGGGGTGTTCCGATTGGCTCGTGGATATGCAATTGCCAAGACTTATCGGCTTGATACCACTGGTGATGCAATTGTAGGATACAATGTCACTGTAACTCACCAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

226

Amino Acids

24.66

Weight (kDa)

8.86

Isoelectric Point (pI)

24.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 80 - 222 1.1e-55 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 34, 137, 312, 368
AcoI YGGCCR 3 cut(s) 357, 436, 462
AcsI RAATTY 1 cut(s) 446
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 1 cut(s) 297
AfiI CCNNNNNNNGG 5 cut(s) 342, 343, 460, 547, 563
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 4 cut(s) 18, 28, 206, 496
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AloI GAACNNNNNNTCC 2 cut(s) 476, 508
AluBI AGCT 8 cut(s) 11, 131, 185, 400, 471, 504, 533, 678
AluI AGCT 8 cut(s) 11, 131, 185, 400, 471, 504, 533, 678
Alw26I GTCTC 2 cut(s) 227, 371
AoxI GGCC 6 cut(s) 171, 219, 357, 436, 451, 462
ApoI RAATTY 1 cut(s) 446
AspLEI GCGC 1 cut(s) 415
AspS9I GGNCC 1 cut(s) 385
AsuC2I CCSGG 1 cut(s) 542
AsuHPI GGTGA 3 cut(s) 148, 647, 662
AsuNHI GCTAGC 1 cut(s) 7
AvaII GGWCC 1 cut(s) 385
BalI TGGCCA 1 cut(s) 464
BanII GRGCYC 1 cut(s) 349
BauI CACGAG 2 cut(s) 401, 591
BccI CCATC 2 cut(s) 163, 479
BceAI ACGGC 1 cut(s) 278
BciVI GTATCC 1 cut(s) 644
BclI TGATCA 1 cut(s) 153
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 2 cut(s) 227, 371
BfaI CTAG 3 cut(s) 8, 12, 679
BfuI GTATCC 1 cut(s) 644
BisI GCNGC 2 cut(s) 137, 312
BlsI GCNGC 2 cut(s) 138, 313
Bme1390I CCNGG 1 cut(s) 542
Bme18I GGWCC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 385
BmiI GGNNCC 1 cut(s) 387
BmrFI CCNGG 1 cut(s) 542
BmrI ACTGGG 2 cut(s) 282, 582
BmsI GCATC 1 cut(s) 628
BmtI GCTAGC 1 cut(s) 11
BmuI ACTGGG 2 cut(s) 282, 582
BpmI CTGGAG 1 cut(s) 233
BpuMI CCSGG 1 cut(s) 542
BsaAI YACGTR 1 cut(s) 299
BsaHI GRCGYC 1 cut(s) 380
BsaI GGTCTC 1 cut(s) 227
Bsc4I CCNNNNNNNGG 5 cut(s) 342, 343, 460, 547, 563
Bse118I RCCGGY 1 cut(s) 556
Bse1I ACTGG 4 cut(s) 250, 288, 577, 637
BseGI GGATG 2 cut(s) 431, 490
BseLI CCNNNNNNNGG 5 cut(s) 342, 343, 460, 547, 563
BseMII CTCAG 2 cut(s) 177, 366
BseNI ACTGG 4 cut(s) 250, 288, 577, 637
BseRI GAGGAG 1 cut(s) 68
BseYI CCCAGC 1 cut(s) 529
BshFI GGCC 6 cut(s) 173, 221, 359, 438, 453, 464
BsiSI CCGG 4 cut(s) 383, 435, 542, 557
BsiWI CGTACG 1 cut(s) 295
BslFI GGGAC 2 cut(s) 313, 420
BslI CCNNNNNNNGG 5 cut(s) 342, 343, 460, 547, 563
BsmAI GTCTC 2 cut(s) 227, 371
BsmBI CGTCTC 1 cut(s) 371
BsmFI GGGAC 2 cut(s) 313, 420
BsmI GAATGC 1 cut(s) 23
BsnI GGCC 6 cut(s) 173, 221, 359, 438, 453, 464
Bso31I GGTCTC 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 349
Bsp143I GATC 1 cut(s) 153
BspACI CCGC 4 cut(s) 34, 137, 312, 368
BspANI GGCC 6 cut(s) 173, 221, 359, 438, 453, 464
BspCNI CTCAG 2 cut(s) 178, 367
BspLI GGNNCC 1 cut(s) 387
BspOI GCTAGC 1 cut(s) 11
BspTNI GGTCTC 1 cut(s) 227
BsrFI RCCGGY 1 cut(s) 556
BsrI ACTGG 4 cut(s) 250, 288, 577, 637
BssAI RCCGGY 1 cut(s) 556
BssMI GATC 1 cut(s) 153
BssNI GRCGYC 1 cut(s) 380
BssSI CACGAG 2 cut(s) 401, 591
Bst2BI CACGAG 2 cut(s) 401, 591
Bst4CI ACNGT 3 cut(s) 238, 271, 664
BstACI GRCGYC 1 cut(s) 380
BstBAI YACGTR 1 cut(s) 299
BstC8I GCNNGC 2 cut(s) 9, 440
BstDEI CTNAG 2 cut(s) 186, 375
BstF5I GGATG 2 cut(s) 431, 490
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 2 cut(s) 227, 371
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 3 cut(s) 128, 191, 311
BstSCI CCNGG 1 cut(s) 540
BstXI CCANNNNNNTGG 2 cut(s) 229, 403
BsuI GTATCC 1 cut(s) 644
BsuRI GGCC 6 cut(s) 173, 221, 359, 438, 453, 464
BtsCI GGATG 2 cut(s) 431, 490
BtsIMutI CAGTG 4 cut(s) 280, 295, 630, 660
Cac8I GCNNGC 2 cut(s) 9, 440
CfoI GCGC 1 cut(s) 415
Cfr10I RCCGGY 1 cut(s) 556
Cfr13I GGNCC 1 cut(s) 385
CseI GACGC 1 cut(s) 388
Csp6I GTAC 1 cut(s) 296
CviAII CATG 2 cut(s) 355, 466
CviQI GTAC 1 cut(s) 296
DdeI CTNAG 2 cut(s) 186, 375
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
EaeI YGGCCR 3 cut(s) 357, 436, 462
Eco24I GRGCYC 1 cut(s) 349
Eco31I GGTCTC 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 385
EcoT38I GRGCYC 1 cut(s) 349
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 2 cut(s) 358, 469
FaiI YATR 5 cut(s) 356, 426, 461, 467, 600
FaqI GGGAC 2 cut(s) 313, 420
FatI CATG 2 cut(s) 354, 465
FauI CCCGC 2 cut(s) 41, 375
FbaI TGATCA 1 cut(s) 153
Fnu4HI GCNGC 2 cut(s) 137, 312
FokI GGATG 2 cut(s) 418, 497
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 2 cut(s) 137, 312
FspBI CTAG 3 cut(s) 8, 12, 679
GlaI GCGC 1 cut(s) 414
GluI GCNGC 2 cut(s) 137, 312
GsaI CCCAGC 1 cut(s) 533
GsuI CTGGAG 1 cut(s) 233
HaeIII GGCC 6 cut(s) 173, 221, 359, 438, 453, 464
HapII CCGG 4 cut(s) 383, 435, 542, 557
HgaI GACGC 1 cut(s) 388
HhaI GCGC 1 cut(s) 415
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 2 cut(s) 358, 469
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HinfI GANTC 2 cut(s) 49, 197
HpaII CCGG 4 cut(s) 383, 435, 542, 557
HphI GGTGA 3 cut(s) 148, 647, 662
Hpy166II GTNNAC 1 cut(s) 480
Hpy188I TCNGA 1 cut(s) 584
Hpy188III TCNNGA 2 cut(s) 88, 548
Hpy8I GTNNAC 1 cut(s) 480
HpyCH4III ACNGT 3 cut(s) 238, 271, 664
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 3 cut(s) 23, 602, 641
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 191, 311
HpyF3I CTNAG 2 cut(s) 186, 375
HpySE526I ACGT 1 cut(s) 298
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 2 cut(s) 358, 469
HspAI GCGC 1 cut(s) 413
Ksp22I TGATCA 1 cut(s) 153
Kzo9I GATC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 509
LweI GCATC 1 cut(s) 628
MaeI CTAG 3 cut(s) 8, 12, 679
MaeII ACGT 1 cut(s) 298
MaeIII GTNAC 3 cut(s) 238, 658, 664
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 1 cut(s) 292
MfeI CAATTG 2 cut(s) 603, 642
MhlI GDGCHC 1 cut(s) 349
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 5 cut(s) 106, 391, 446, 603, 642
MluNI TGGCCA 1 cut(s) 464
MlyI GAGTC 2 cut(s) 43, 206
MnlI CCTC 6 cut(s) 89, 169, 211, 257, 347, 464
Mox20I TGGCCA 1 cut(s) 464
MscI TGGCCA 1 cut(s) 464
MseI TTAA 2 cut(s) 45, 149
Msp20I TGGCCA 1 cut(s) 464
MspI CCGG 4 cut(s) 383, 435, 542, 557
MspR9I CCNGG 1 cut(s) 542
MunI CAATTG 2 cut(s) 603, 642
Mva1269I GAATGC 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 128, 191, 311
NciI CCSGG 1 cut(s) 542
NdeII GATC 1 cut(s) 153
NheI GCTAGC 1 cut(s) 7
NlaIII CATG 2 cut(s) 358, 469
NlaIV GGNNCC 1 cut(s) 387
NmuCI GTSAC 1 cut(s) 658
PcsI WCGNNNNNNNCGW 1 cut(s) 488
PctI GAATGC 1 cut(s) 23
Pfl23II CGTACG 1 cut(s) 295
PflFI GACNNNGTC 1 cut(s) 269
PkrI GCNGC 2 cut(s) 138, 313
PleI GAGTC 2 cut(s) 43, 205
PpsI GAGTC 2 cut(s) 43, 205
Ppu21I YACGTR 1 cut(s) 299
PspFI CCCAGC 1 cut(s) 529
PspLI CGTACG 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 387
PspPI GGNCC 1 cut(s) 385
PsyI GACNNNGTC 1 cut(s) 269
RsaI GTAC 1 cut(s) 297
RsaNI GTAC 1 cut(s) 296
SaqAI TTAA 2 cut(s) 45, 149
SatI GCNGC 2 cut(s) 137, 312
Sau3AI GATC 1 cut(s) 153
Sau96I GGNCC 1 cut(s) 385
SchI GAGTC 2 cut(s) 43, 206
ScrFI CCNGG 1 cut(s) 542
SduI GDGCHC 1 cut(s) 349
SfaNI GCATC 1 cut(s) 628
SinI GGWCC 1 cut(s) 385
Sse9I AATT 5 cut(s) 106, 391, 446, 603, 642
SsiI CCGC 4 cut(s) 34, 137, 312, 368
SspMI CTAG 3 cut(s) 8, 12, 679
StyD4I CCNGG 1 cut(s) 540
TaaI ACNGT 3 cut(s) 238, 271, 664
TaiI ACGT 1 cut(s) 301
TasI AATT 5 cut(s) 106, 391, 446, 603, 642
TauI GCSGC 2 cut(s) 139, 314
Tru1I TTAA 2 cut(s) 45, 149
Tru9I TTAA 2 cut(s) 45, 149
TscAI CASTG 4 cut(s) 280, 295, 637, 667
TseFI GTSAC 1 cut(s) 658
Tsp45I GTSAC 1 cut(s) 658
TspGWI ACGGA 3 cut(s) 194, 496, 497
TspRI CASTG 4 cut(s) 280, 295, 637, 667
Tth111I GACNNNGTC 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 385
XapI RAATTY 1 cut(s) 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 446
XspI CTAG 3 cut(s) 8, 12, 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.