FvH4_2g01690

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003188
Physical Location & Seq
Reverse (-)
1507871 .. 1508667
797 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01690.t1

Sequence Viewer

Length: 573 bp
ATGAAGCAGAAAATGGCAAACAACACAGTTGCGACTCTGATGATGATTGTCGTCATCTCCTTGGCGATGGCCTTCGCAGAAGCCAAGTGGGGAGGGTCAGTCGAGGCCAAAAAGGAAACGAAGCCGTCTGAGAGAGTAACCAACCTCCAGTTCTACTTCCACGACACAGTCAGTGGGAAGAACCCTAGTGCCATACGTGTAGCTCAGGCCCCGGACACCGAAAAATCTCCTACCCTTTTCGGGGCACTGCTCATGGCCGATGACCCGCTCACCGAGACGCCCGATCCCGATTCCAAGCTCGTGGGGCGGGCCCAAGGGCTGTACGGGTCATCCGGCCAGCATGAGCTTGGCCTGATAATGGCCATGAGTTTTGCGTTCACGGATGGTACGTATAATGGTAGCTCCATTAGTATTTTTGGTAAAAACCCGGCTCTGAACCCGGTTCGGGAGTTGCCCGTTGTGGGTGGAACCGGGATGTTCCGGCTGGCTCGTGGATATGCAATTGCTTGTACTCATTGGTTTGATGTTAGCGGTGATGCAATCGTAGGATACAATGTCACTGTAATTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

191

Amino Acids

20.35

Weight (kDa)

6.41

Isoelectric Point (pI)

29.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 46 - 188 2.8e-55 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 268
AciI CCGC 3 cut(s) 266, 307, 531
AclWI GGATC 1 cut(s) 278
AcoI YGGCCR 3 cut(s) 255, 334, 360
AcyI GRCGYC 1 cut(s) 278
AfaI GTAC 3 cut(s) 323, 388, 511
AfiI CCNNNNNNNGG 5 cut(s) 240, 241, 358, 445, 461
AflIII ACRYGT 1 cut(s) 196
AjuI GAANNNNNNNTTGG 2 cut(s) 134, 166
AluBI AGCT 4 cut(s) 203, 298, 346, 402
AluI AGCT 4 cut(s) 203, 298, 346, 402
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 1 cut(s) 278
AoxI GGCC 8 cut(s) 69, 105, 207, 255, 309, 334, 349, 360
ApaI GGGCCC 1 cut(s) 313
AspS9I GGNCC 3 cut(s) 208, 309, 310
AsuC2I CCSGG 4 cut(s) 212, 428, 440, 472
AsuHPI GGTGA 2 cut(s) 262, 545
BaeGI GKGCMC 2 cut(s) 247, 313
BalI TGGCCA 1 cut(s) 362
BanII GRGCYC 1 cut(s) 313
BauI CACGAG 2 cut(s) 299, 489
BccI CCATC 2 cut(s) 61, 377
BceAI ACGGC 1 cut(s) 109
BciVI GTATCC 1 cut(s) 542
BcnI CCSGG 4 cut(s) 212, 428, 440, 472
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 2 cut(s) 186, 571
BfuI GTATCC 1 cut(s) 542
Bme1390I CCNGG 4 cut(s) 212, 428, 440, 472
BmgT120I GGNCC 3 cut(s) 208, 309, 310
BmiI GGNNCC 3 cut(s) 210, 311, 469
BmrFI CCNGG 4 cut(s) 212, 428, 440, 472
BmsI GCATC 1 cut(s) 526
BpmI CTGGAG 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 204
BpuMI CCSGG 4 cut(s) 212, 428, 440, 472
BsaAI YACGTR 2 cut(s) 197, 390
BsaHI GRCGYC 1 cut(s) 278
BsaJI CCNNGG 3 cut(s) 60, 210, 313
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 5 cut(s) 240, 241, 358, 445, 461
Bse1I ACTGG 1 cut(s) 148
BseDI CCNNGG 3 cut(s) 60, 210, 313
BseGI GGATG 3 cut(s) 329, 388, 480
BseLI CCNNNNNNNGG 5 cut(s) 240, 241, 358, 445, 461
BseMII CTCAG 2 cut(s) 120, 218
BseNI ACTGG 1 cut(s) 148
BseSI GKGCMC 2 cut(s) 247, 313
BshFI GGCC 8 cut(s) 71, 107, 209, 257, 311, 336, 351, 362
BsiSI CCGG 6 cut(s) 212, 333, 428, 440, 471, 481
BslI CCNNNNNNNGG 5 cut(s) 240, 241, 358, 445, 461
BsmAI GTCTC 1 cut(s) 269
BsmBI CGTCTC 1 cut(s) 269
BsnI GGCC 8 cut(s) 71, 107, 209, 257, 311, 336, 351, 362
Bsp120I GGGCCC 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 247, 313
Bsp143I GATC 1 cut(s) 283
BspACI CCGC 3 cut(s) 266, 307, 531
BspANI GGCC 8 cut(s) 71, 107, 209, 257, 311, 336, 351, 362
BspCNI CTCAG 2 cut(s) 121, 217
BspLI GGNNCC 3 cut(s) 210, 311, 469
BspPI GGATC 1 cut(s) 278
BsrBI CCGCTC 1 cut(s) 268
BsrI ACTGG 1 cut(s) 148
BssECI CCNNGG 3 cut(s) 60, 210, 313
BssMI GATC 1 cut(s) 283
BssNI GRCGYC 1 cut(s) 278
BssSI CACGAG 2 cut(s) 299, 489
BssT1I CCWWGG 2 cut(s) 60, 313
Bst2BI CACGAG 2 cut(s) 299, 489
Bst4CI ACNGT 3 cut(s) 28, 169, 562
BstACI GRCGYC 1 cut(s) 278
BstBAI YACGTR 2 cut(s) 197, 390
BstC8I GCNNGC 3 cut(s) 309, 338, 486
BstDEI CTNAG 2 cut(s) 129, 204
BstF5I GGATG 3 cut(s) 329, 388, 480
BstKTI GATC 1 cut(s) 286
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 1 cut(s) 283
BstMWI GCNNNNNNNGC 1 cut(s) 304
BstSCI CCNGG 4 cut(s) 210, 426, 438, 470
BstSLI GKGCMC 2 cut(s) 247, 313
BstSNI TACGTA 1 cut(s) 390
BstXI CCANNNNNNTGG 1 cut(s) 301
BsuI GTATCC 1 cut(s) 542
BsuRI GGCC 8 cut(s) 71, 107, 209, 257, 311, 336, 351, 362
BtgZI GCGATG 1 cut(s) 80
BtsCI GGATG 3 cut(s) 329, 388, 480
BtsI GCAGTG 1 cut(s) 245
BtsIMutI CAGTG 3 cut(s) 178, 245, 558
Cac8I GCNNGC 3 cut(s) 309, 338, 486
Cfr13I GGNCC 3 cut(s) 208, 309, 310
CseI GACGC 1 cut(s) 286
Csp6I GTAC 3 cut(s) 322, 387, 510
CviAII CATG 3 cut(s) 253, 341, 364
CviQI GTAC 3 cut(s) 322, 387, 510
DdeI CTNAG 2 cut(s) 129, 204
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
EaeI YGGCCR 3 cut(s) 255, 334, 360
Eco105I TACGTA 1 cut(s) 390
Eco130I CCWWGG 2 cut(s) 60, 313
Eco24I GRGCYC 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 208
EcoT14I CCWWGG 2 cut(s) 60, 313
EcoT38I GRGCYC 1 cut(s) 313
ErhI CCWWGG 2 cut(s) 60, 313
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 3 cut(s) 256, 344, 367
FaiI YATR 6 cut(s) 194, 254, 342, 365, 393, 498
FatI CATG 3 cut(s) 252, 340, 363
FauI CCCGC 2 cut(s) 273, 300
FokI GGATG 3 cut(s) 316, 395, 487
FriOI GRGCYC 1 cut(s) 313
FspBI CTAG 2 cut(s) 186, 571
GsuI CTGGAG 1 cut(s) 131
HaeIII GGCC 8 cut(s) 71, 107, 209, 257, 311, 336, 351, 362
HapII CCGG 6 cut(s) 212, 333, 428, 440, 471, 481
HgaI GACGC 1 cut(s) 286
Hin1I GRCGYC 1 cut(s) 278
Hin1II CATG 3 cut(s) 256, 344, 367
HinfI GANTC 2 cut(s) 34, 290
HpaII CCGG 6 cut(s) 212, 333, 428, 440, 471, 481
HphI GGTGA 2 cut(s) 262, 545
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 3 cut(s) 39, 130, 435
Hpy188III TCNNGA 2 cut(s) 287, 446
Hpy8I GTNNAC 1 cut(s) 378
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 3 cut(s) 28, 169, 562
HpyCH4IV ACGT 2 cut(s) 196, 389
HpyCH4V TGCA 2 cut(s) 500, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 304
HpyF3I CTNAG 2 cut(s) 129, 204
HpySE526I ACGT 2 cut(s) 196, 389
Hsp92I GRCGYC 1 cut(s) 278
Hsp92II CATG 3 cut(s) 256, 344, 367
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 1 cut(s) 407
LweI GCATC 1 cut(s) 526
MaeI CTAG 2 cut(s) 186, 571
MaeII ACGT 2 cut(s) 196, 389
MaeIII GTNAC 2 cut(s) 136, 556
MalI GATC 1 cut(s) 285
MbiI CCGCTC 1 cut(s) 268
MboI GATC 1 cut(s) 283
MboII GAAGA 1 cut(s) 190
MfeI CAATTG 1 cut(s) 501
MhlI GDGCHC 2 cut(s) 247, 313
MlsI TGGCCA 1 cut(s) 362
MluCI AATT 2 cut(s) 501, 564
MluNI TGGCCA 1 cut(s) 362
MlyI GAGTC 1 cut(s) 28
MnlI CCTC 3 cut(s) 86, 97, 155
Mox20I TGGCCA 1 cut(s) 362
MscI TGGCCA 1 cut(s) 362
Msp20I TGGCCA 1 cut(s) 362
MspI CCGG 6 cut(s) 212, 333, 428, 440, 471, 481
MspR9I CCNGG 4 cut(s) 212, 428, 440, 472
MunI CAATTG 1 cut(s) 501
MwoI GCNNNNNNNGC 1 cut(s) 304
NciI CCSGG 4 cut(s) 212, 428, 440, 472
NdeII GATC 1 cut(s) 283
NlaIII CATG 3 cut(s) 256, 344, 367
NlaIV GGNNCC 3 cut(s) 210, 311, 469
NmuCI GTSAC 1 cut(s) 556
PcsI WCGNNNNNNNCGW 1 cut(s) 386
PfeI GAWTC 1 cut(s) 290
PflFI GACNNNGTC 1 cut(s) 167
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
Ppu21I YACGTR 2 cut(s) 197, 390
PspN4I GGNNCC 3 cut(s) 210, 311, 469
PspOMI GGGCCC 1 cut(s) 309
PspPI GGNCC 3 cut(s) 208, 309, 310
PsyI GACNNNGTC 1 cut(s) 167
RsaI GTAC 3 cut(s) 323, 388, 511
RsaNI GTAC 3 cut(s) 322, 387, 510
Sau3AI GATC 1 cut(s) 283
Sau96I GGNCC 3 cut(s) 208, 309, 310
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 4 cut(s) 212, 428, 440, 472
SduI GDGCHC 2 cut(s) 247, 313
SetI ASST 7 cut(s) 147, 199, 205, 300, 348, 392, 404
SfaNI GCATC 1 cut(s) 526
SnaBI TACGTA 1 cut(s) 390
Sse9I AATT 2 cut(s) 501, 564
SsiI CCGC 3 cut(s) 266, 307, 531
SspMI CTAG 2 cut(s) 186, 571
StyD4I CCNGG 4 cut(s) 210, 426, 438, 470
StyI CCWWGG 2 cut(s) 60, 313
TaaI ACNGT 3 cut(s) 28, 169, 562
TaiI ACGT 2 cut(s) 199, 392
TaqI TCGA 1 cut(s) 102
TasI AATT 2 cut(s) 501, 564
TatI WGTACW 1 cut(s) 509
TfiI GAWTC 1 cut(s) 290
TscAI CASTG 3 cut(s) 178, 252, 565
TseFI GTSAC 1 cut(s) 556
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 3 cut(s) 178, 252, 565
Tth111I GACNNNGTC 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XspI CTAG 2 cut(s) 186, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.