Prupe.8G162100_v2.0.a1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0003188
Physical Location & Seq
Forward (+)
16989592 .. 16991339
1748 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G162100.1

Sequence Viewer

Length: 579 bp
ATGGCAAACTCAGCAACCCCTCTGATTTTGCTCATGCTCATCTCTCTACTGGCAGCCATTGAAGGAGCTCAAAACTCCACAGAGCTGAAGTGGGCCAAGAAGCTCAAGACCAGAAAGAAGACGACGTCGTCGGAGACAGTGACCAACCTCCAGTTCTACTTCCACGACACTCTCAGCGGGAAGACCCCAAGCGCCGTGCGTGTAGCCCAAGCCACAGACACCGAGAAGTCCCCCACCCTTTTCGGAGCTTTGCTTATGGCGGATGACCCGCTTACTGAAACACCCGACCCGAACTCCAAGCTCGTGGGTCGGGCCCAAGGACTGTACGGGTCATCTTGCCAGCAAGAGCTTGGGCTGATTATGGCCATGAACTTTGGGTTCACGGATGGCATGTACAACGGTAGCTCTATTAGTATTCTCGGTAAGAACTCGGCTATGAACCCGGTTCGGGAGATGCCGGTCGTGGCTGGAACCGGGGTTTTCCGGTTGGGCCGGGGGTATGCGATTGCCAAGACACATTGGATTGATTTTACCACGGGTGATGCAATTGTTGGGTACAATGTTACTGTAATTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

193

Amino Acids

20.61

Weight (kDa)

8.67

Isoelectric Point (pI)

25.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 127
AatII GACGTC 1 cut(s) 128
AciI CCGC 3 cut(s) 177, 260, 269
AcoI YGGCCR 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 3 cut(s) 326, 395, 557
AfiI CCNNNNNNNGG 1 cut(s) 448
AgsI TTSAA 1 cut(s) 62
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
AluBI AGCT 7 cut(s) 68, 85, 103, 248, 301, 349, 405
AluI AGCT 7 cut(s) 68, 85, 103, 248, 301, 349, 405
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 1 cut(s) 128
AoxI GGCC 4 cut(s) 93, 312, 363, 490
ApaI GGGCCC 1 cut(s) 316
ApeKI GCWGC 1 cut(s) 53
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 4 cut(s) 93, 312, 313, 490
AsuC2I CCSGG 3 cut(s) 443, 475, 494
AsuHPI GGTGA 1 cut(s) 551
BaeGI GKGCMC 1 cut(s) 316
BalI TGGCCA 1 cut(s) 365
BanII GRGCYC 2 cut(s) 70, 316
BauI CACGAG 1 cut(s) 302
BbsI GAAGAC 2 cut(s) 125, 188
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 65
BccI CCATC 1 cut(s) 380
BceAI ACGGC 1 cut(s) 179
BcnI CCSGG 3 cut(s) 443, 475, 494
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 1 cut(s) 577
BfoI RGCGCY 1 cut(s) 195
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
Bme1390I CCNGG 3 cut(s) 443, 475, 494
BmgT120I GGNCC 4 cut(s) 93, 312, 313, 490
BmiI GGNNCC 2 cut(s) 314, 472
BmrFI CCNGG 3 cut(s) 443, 475, 494
BmsI GCATC 2 cut(s) 444, 532
BpiI GAAGAC 2 cut(s) 125, 188
BpmI CTGGAG 1 cut(s) 134
BpuEI CTTGAG 1 cut(s) 89
BpuMI CCSGG 3 cut(s) 443, 475, 494
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 4 cut(s) 316, 474, 493, 534
BsaWI WCCGGW 1 cut(s) 483
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 1 cut(s) 448
Bse118I RCCGGY 1 cut(s) 457
Bse1I ACTGG 2 cut(s) 54, 151
BseDI CCNNGG 4 cut(s) 316, 474, 493, 534
BseGI GGATG 2 cut(s) 268, 391
BseLI CCNNNNNNNGG 1 cut(s) 448
BseMII CTCAG 2 cut(s) 24, 187
BseNI ACTGG 2 cut(s) 54, 151
BseSI GKGCMC 1 cut(s) 316
BseXI GCAGC 1 cut(s) 65
Bsh1285I CGRYCG 1 cut(s) 462
BshFI GGCC 4 cut(s) 95, 314, 365, 492
BsiEI CGRYCG 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 70
BsiSI CCGG 5 cut(s) 443, 458, 474, 484, 493
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 1 cut(s) 448
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 214
BsnI GGCC 4 cut(s) 95, 314, 365, 492
Bsp120I GGGCCC 1 cut(s) 312
Bsp1286I GDGCHC 2 cut(s) 70, 316
Bsp1407I TGTACA 1 cut(s) 393
BspACI CCGC 3 cut(s) 177, 260, 269
BspANI GGCC 4 cut(s) 95, 314, 365, 492
BspCNI CTCAG 2 cut(s) 23, 186
BspLI GGNNCC 2 cut(s) 314, 472
BsrFI RCCGGY 1 cut(s) 457
BsrGI TGTACA 1 cut(s) 393
BsrI ACTGG 2 cut(s) 54, 151
BssAI RCCGGY 1 cut(s) 457
BssECI CCNNGG 4 cut(s) 316, 474, 493, 534
BssNI GRCGYC 1 cut(s) 125
BssSI CACGAG 1 cut(s) 302
BssT1I CCWWGG 1 cut(s) 316
Bst2BI CACGAG 1 cut(s) 302
Bst4CI ACNGT 4 cut(s) 139, 324, 401, 568
BstACI GRCGYC 1 cut(s) 125
BstAUI TGTACA 1 cut(s) 393
BstC8I GCNNGC 1 cut(s) 341
BstDEI CTNAG 2 cut(s) 10, 173
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 2 cut(s) 268, 391
BstH2I RGCGCY 1 cut(s) 195
BstHHI GCGC 1 cut(s) 194
BstMAI GTCTC 1 cut(s) 128
BstMCI CGRYCG 1 cut(s) 462
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 394
BstSCI CCNGG 3 cut(s) 441, 473, 492
BstSLI GKGCMC 1 cut(s) 316
BstV1I GCAGC 1 cut(s) 65
BstV2I GAAGAC 2 cut(s) 125, 188
BstXI CCANNNNNNTGG 1 cut(s) 304
BsuRI GGCC 4 cut(s) 95, 314, 365, 492
BtgI CCRYGG 1 cut(s) 534
BtsCI GGATG 2 cut(s) 268, 391
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 341
CfoI GCGC 1 cut(s) 194
Cfr10I RCCGGY 1 cut(s) 457
Cfr13I GGNCC 4 cut(s) 93, 312, 313, 490
Csp6I GTAC 3 cut(s) 325, 394, 556
CviAII CATG 3 cut(s) 34, 367, 391
CviQI GTAC 3 cut(s) 325, 394, 556
DdeI CTNAG 2 cut(s) 10, 173
DrdI GACNNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 363
EciI GGCGGA 1 cut(s) 275
Ecl136II GAGCTC 1 cut(s) 68
Eco130I CCWWGG 1 cut(s) 316
Eco24I GRGCYC 2 cut(s) 70, 316
Eco53kI GAGCTC 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 68
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 2 cut(s) 70, 316
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 3 cut(s) 37, 370, 394
FaiI YATR 7 cut(s) 35, 257, 362, 368, 392, 437, 501
FaqI GGGAC 1 cut(s) 214
FatI CATG 3 cut(s) 33, 366, 390
FauI CCCGC 2 cut(s) 170, 276
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 2 cut(s) 275, 398
FriOI GRGCYC 2 cut(s) 70, 316
Fsp4HI GCNGC 1 cut(s) 54
FspBI CTAG 1 cut(s) 577
GlaI GCGC 1 cut(s) 193
GluI GCNGC 1 cut(s) 54
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 195
HaeIII GGCC 4 cut(s) 95, 314, 365, 492
HapII CCGG 5 cut(s) 443, 458, 474, 484, 493
HhaI GCGC 1 cut(s) 194
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 3 cut(s) 37, 370, 394
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HpaII CCGG 5 cut(s) 443, 458, 474, 484, 493
HphI GGTGA 1 cut(s) 551
Hpy166II GTNNAC 1 cut(s) 381
Hpy188I TCNGA 3 cut(s) 24, 133, 245
Hpy188III TCNNGA 2 cut(s) 106, 449
Hpy8I GTNNAC 1 cut(s) 381
Hpy99I CGWCG 3 cut(s) 127, 130, 133
HpyAV CCTTC 1 cut(s) 56
HpyCH4III ACNGT 4 cut(s) 139, 324, 401, 568
HpyCH4IV ACGT 1 cut(s) 125
HpyCH4V TGCA 1 cut(s) 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 2 cut(s) 10, 173
HpySE526I ACGT 1 cut(s) 125
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 3 cut(s) 37, 370, 394
HspAI GCGC 1 cut(s) 192
LmnI GCTCC 2 cut(s) 65, 245
Lsp1109I GCAGC 1 cut(s) 65
LweI GCATC 2 cut(s) 444, 532
MaeI CTAG 1 cut(s) 577
MaeII ACGT 1 cut(s) 125
MaeIII GTNAC 2 cut(s) 139, 562
MboII GAAGA 2 cut(s) 130, 193
MfeI CAATTG 1 cut(s) 546
MhlI GDGCHC 2 cut(s) 70, 316
MlsI TGGCCA 1 cut(s) 365
MluCI AATT 2 cut(s) 546, 570
MluNI TGGCCA 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 2 cut(s) 30, 158
Mox20I TGGCCA 1 cut(s) 365
MscI TGGCCA 1 cut(s) 365
Msp20I TGGCCA 1 cut(s) 365
MspA1I CMGCKG 1 cut(s) 177
MspI CCGG 5 cut(s) 443, 458, 474, 484, 493
MspR9I CCNGG 3 cut(s) 443, 475, 494
MunI CAATTG 1 cut(s) 546
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 3 cut(s) 443, 475, 494
NlaIII CATG 3 cut(s) 37, 370, 394
NlaIV GGNNCC 2 cut(s) 314, 472
NmeAIII GCCGAG 1 cut(s) 410
NmuCI GTSAC 1 cut(s) 139
NspI RCATGY 1 cut(s) 394
PflFI GACNNNGTC 2 cut(s) 124, 127
PkrI GCNGC 1 cut(s) 55
Psp124BI GAGCTC 1 cut(s) 70
PspN4I GGNNCC 2 cut(s) 314, 472
PspOMI GGGCCC 1 cut(s) 312
PspPI GGNCC 4 cut(s) 93, 312, 313, 490
PsyI GACNNNGTC 2 cut(s) 124, 127
RsaI GTAC 3 cut(s) 326, 395, 557
RsaNI GTAC 3 cut(s) 325, 394, 556
SacI GAGCTC 1 cut(s) 70
SatI GCNGC 1 cut(s) 54
Sau96I GGNCC 4 cut(s) 93, 312, 313, 490
ScrFI CCNGG 3 cut(s) 443, 475, 494
SduI GDGCHC 2 cut(s) 70, 316
SetI ASST 9 cut(s) 70, 87, 105, 128, 150, 250, 303, 351, 407
SfaNI GCATC 2 cut(s) 444, 532
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 2 cut(s) 546, 570
SsiI CCGC 3 cut(s) 177, 260, 269
SspMI CTAG 1 cut(s) 577
SstI GAGCTC 1 cut(s) 70
StyD4I CCNGG 3 cut(s) 441, 473, 492
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 4 cut(s) 139, 324, 401, 568
TaiI ACGT 1 cut(s) 128
TasI AATT 2 cut(s) 546, 570
TatI WGTACW 1 cut(s) 393
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 2 cut(s) 383, 452
TspGWI ACGGA 1 cut(s) 398
TspRI CASTG 1 cut(s) 144
Tth111I GACNNNGTC 2 cut(s) 124, 127
XceI RCATGY 1 cut(s) 394
XcmI CCANNNNNNNNNTGG 1 cut(s) 347
XspI CTAG 1 cut(s) 577
ZraI GACGTC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.