Rw6G002060

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003188
Physical Location & Seq
Reverse (-)
3284397 .. 3285038
642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002060.1

Sequence Viewer

Length: 561 bp
ATGGCAAACAACACTGTTGCGCCTCTGATGATGATGGTCGTCATCTCCTTGGTGATGGCATTCACAGAAGCCAAGTGGGCCGAGTCAGTAGAGGCCAAAAAGGAAGCGACGCCGTCGGAGACAGTAACCAACCTCCAGTTCTTCTTCCACGACACAGTCAGTGGGAAGAACCCTAGTGCCGTACGTGTGGCTCAAGCCTCGAACACCAATAAGTCTCTGACACTTTTCGGGGCTTTACTCATGGCCGATGACCCACTCACTGAGACTCCCGACCCCAATTCCAAGATCGTGGGTCGGGCACAAGGGCTATACGGGTCATCCGGCCAGCAGGAGCTTGGCCTGATTATGGCTATGAGCTTTGCGTTCACGGATGGTCCTTACAGTGGTAGTTCCATTAGTATATTTGGTAAAAACCCGGCACTGAACCCAGTTCGAGAGTTGCCGGTTGTGGGTGGAACTGGGGTGTTCCGGCTGGCTCGTGGATATGCAATTGCCCATACTCATTGGTTTGATCTTAGTGGTGATGCAATTGTAGGATACAATGTCACTGTGATTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

186

Amino Acids

19.81

Weight (kDa)

5.75

Isoelectric Point (pI)

21.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 42 - 184 1.1e-54 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 243, 322
AcyI GRCGYC 1 cut(s) 110
AfaI GTAC 1 cut(s) 183
AfiI CCNNNNNNNGG 3 cut(s) 346, 383, 449
AflIII ACRYGT 1 cut(s) 184
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 2 cut(s) 334, 357
AluI AGCT 2 cut(s) 334, 357
Alw26I GTCTC 3 cut(s) 113, 219, 257
AoxI GGCC 5 cut(s) 78, 93, 243, 322, 337
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 2 cut(s) 78, 374
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 2 cut(s) 64, 533
AvaII GGWCC 1 cut(s) 374
BaeGI GKGCMC 1 cut(s) 301
BauI CACGAG 1 cut(s) 477
BccI CCATC 3 cut(s) 28, 49, 365
BceAI ACGGC 2 cut(s) 97, 164
BciVI GTATCC 1 cut(s) 530
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 3 cut(s) 113, 219, 257
BfaI CTAG 2 cut(s) 174, 559
BfuI GTATCC 1 cut(s) 530
BglI GCCNNNNNGGC 1 cut(s) 77
Bme1390I CCNGG 1 cut(s) 416
Bme18I GGWCC 1 cut(s) 374
BmgT120I GGNCC 2 cut(s) 78, 374
BmrFI CCNGG 1 cut(s) 416
BmrI ACTGGG 2 cut(s) 422, 468
BmsI GCATC 1 cut(s) 514
BmuI ACTGGG 2 cut(s) 422, 468
BpmI CTGGAG 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 177
BpuMI CCSGG 1 cut(s) 416
BsaAI YACGTR 1 cut(s) 185
BsaHI GRCGYC 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 48
BsaXI ACNNNNNCTCC 2 cut(s) 250, 280
Bsc4I CCNNNNNNNGG 3 cut(s) 346, 383, 449
Bse118I RCCGGY 1 cut(s) 442
Bse1I ACTGG 3 cut(s) 136, 428, 463
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 2 cut(s) 317, 376
BseLI CCNNNNNNNGG 3 cut(s) 346, 383, 449
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 3 cut(s) 136, 428, 463
BseSI GKGCMC 1 cut(s) 301
BshFI GGCC 5 cut(s) 80, 95, 245, 324, 339
BsiSI CCGG 4 cut(s) 321, 416, 443, 469
BsiWI CGTACG 1 cut(s) 181
BslI CCNNNNNNNGG 3 cut(s) 346, 383, 449
BsmAI GTCTC 3 cut(s) 113, 219, 257
BsmI GAATGC 1 cut(s) 59
BsnI GGCC 5 cut(s) 80, 95, 245, 324, 339
Bsp1286I GDGCHC 1 cut(s) 301
Bsp143I GATC 2 cut(s) 285, 511
BspANI GGCC 5 cut(s) 80, 95, 245, 324, 339
BspCNI CTCAG 1 cut(s) 253
BsrFI RCCGGY 1 cut(s) 442
BsrI ACTGG 3 cut(s) 136, 428, 463
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 2 cut(s) 285, 511
BssNI GRCGYC 1 cut(s) 110
BssSI CACGAG 1 cut(s) 477
BssT1I CCWWGG 1 cut(s) 48
Bst2BI CACGAG 1 cut(s) 477
Bst4CI ACNGT 5 cut(s) 16, 124, 157, 383, 550
BstACI GRCGYC 1 cut(s) 110
BstBAI YACGTR 1 cut(s) 185
BstC8I GCNNGC 2 cut(s) 326, 474
BstDEI CTNAG 2 cut(s) 261, 515
BstF5I GGATG 2 cut(s) 317, 376
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 2 cut(s) 288, 514
BstMAI GTCTC 3 cut(s) 113, 219, 257
BstMBI GATC 2 cut(s) 285, 511
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 414
BstSLI GKGCMC 1 cut(s) 301
BstXI CCANNNNNNTGG 1 cut(s) 289
BsuI GTATCC 1 cut(s) 530
BsuRI GGCC 5 cut(s) 80, 95, 245, 324, 339
BtsCI GGATG 2 cut(s) 317, 376
BtsIMutI CAGTG 6 cut(s) 12, 166, 258, 388, 419, 546
Cac8I GCNNGC 2 cut(s) 326, 474
CfoI GCGC 1 cut(s) 22
Cfr10I RCCGGY 1 cut(s) 442
Cfr13I GGNCC 2 cut(s) 78, 374
CseI GACGC 1 cut(s) 118
Csp6I GTAC 1 cut(s) 182
CviAII CATG 1 cut(s) 241
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 2 cut(s) 261, 515
DpnI GATC 2 cut(s) 287, 513
DpnII GATC 2 cut(s) 285, 511
EaeI YGGCCR 2 cut(s) 243, 322
Eco130I CCWWGG 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 374
EcoT14I CCWWGG 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 1 cut(s) 244
FaiI YATR 7 cut(s) 242, 310, 347, 353, 401, 486, 498
FatI CATG 1 cut(s) 240
FokI GGATG 2 cut(s) 304, 383
FspBI CTAG 2 cut(s) 174, 559
GlaI GCGC 1 cut(s) 21
GsuI CTGGAG 1 cut(s) 119
HaeIII GGCC 5 cut(s) 80, 95, 245, 324, 339
HapII CCGG 4 cut(s) 321, 416, 443, 469
HgaI GACGC 1 cut(s) 118
HhaI GCGC 1 cut(s) 22
Hin1I GRCGYC 1 cut(s) 110
Hin1II CATG 1 cut(s) 244
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HinfI GANTC 3 cut(s) 83, 265, 553
HpaII CCGG 4 cut(s) 321, 416, 443, 469
HphI GGTGA 2 cut(s) 64, 533
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 3 cut(s) 27, 118, 219
Hpy188III TCNNGA 2 cut(s) 269, 434
Hpy8I GTNNAC 1 cut(s) 366
Hpy99I CGWCG 2 cut(s) 112, 118
HpyCH4III ACNGT 5 cut(s) 16, 124, 157, 383, 550
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 2 cut(s) 488, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 2 cut(s) 261, 515
HpySE526I ACGT 1 cut(s) 184
Hsp92I GRCGYC 1 cut(s) 110
Hsp92II CATG 1 cut(s) 244
HspAI GCGC 1 cut(s) 20
Kzo9I GATC 2 cut(s) 285, 511
LmnI GCTCC 1 cut(s) 331
LweI GCATC 1 cut(s) 514
MaeI CTAG 2 cut(s) 174, 559
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 2 cut(s) 124, 544
MalI GATC 2 cut(s) 287, 513
MboI GATC 2 cut(s) 285, 511
MboII GAAGA 3 cut(s) 133, 136, 178
MfeI CAATTG 2 cut(s) 489, 528
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 3 cut(s) 277, 489, 528
MlyI GAGTC 2 cut(s) 92, 259
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 4 cut(s) 33, 85, 143, 208
MspI CCGG 4 cut(s) 321, 416, 443, 469
MspR9I CCNGG 1 cut(s) 416
MunI CAATTG 2 cut(s) 489, 528
Mva1269I GAATGC 1 cut(s) 59
MwoI GCNNNNNNNGC 1 cut(s) 77
NciI CCSGG 1 cut(s) 416
NdeII GATC 2 cut(s) 285, 511
NlaIII CATG 1 cut(s) 244
NmeAIII GCCGAG 1 cut(s) 106
NmuCI GTSAC 1 cut(s) 544
PctI GAATGC 1 cut(s) 59
PfeI GAWTC 1 cut(s) 553
Pfl23II CGTACG 1 cut(s) 181
PflFI GACNNNGTC 2 cut(s) 112, 155
PleI GAGTC 2 cut(s) 91, 259
PpsI GAGTC 2 cut(s) 91, 259
Ppu21I YACGTR 1 cut(s) 185
PspLI CGTACG 1 cut(s) 181
PspPI GGNCC 2 cut(s) 78, 374
PsyI GACNNNGTC 2 cut(s) 112, 155
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
Sau3AI GATC 2 cut(s) 285, 511
Sau96I GGNCC 2 cut(s) 78, 374
SchI GAGTC 2 cut(s) 92, 259
ScrFI CCNGG 1 cut(s) 416
SduI GDGCHC 1 cut(s) 301
SetI ASST 4 cut(s) 135, 187, 336, 359
SfaNI GCATC 1 cut(s) 514
SinI GGWCC 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 192
SmoI CTYRAG 1 cut(s) 192
Sse9I AATT 3 cut(s) 277, 489, 528
SspMI CTAG 2 cut(s) 174, 559
StyD4I CCNGG 1 cut(s) 414
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 5 cut(s) 16, 124, 157, 383, 550
TaiI ACGT 1 cut(s) 187
TaqI TCGA 2 cut(s) 200, 433
TasI AATT 3 cut(s) 277, 489, 528
TfiI GAWTC 1 cut(s) 553
TscAI CASTG 6 cut(s) 19, 166, 265, 388, 426, 553
TseFI GTSAC 1 cut(s) 544
Tsp45I GTSAC 1 cut(s) 544
TspGWI ACGGA 1 cut(s) 383
TspRI CASTG 6 cut(s) 19, 166, 265, 388, 426, 553
Tth111I GACNNNGTC 2 cut(s) 112, 155
VpaK11BI GGWCC 1 cut(s) 374
XcmI CCANNNNNNNNNTGG 1 cut(s) 332
XspI CTAG 2 cut(s) 174, 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.