Rroxscaffold_7G00215710

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003188
Physical Location & Seq
Forward (+)
66273514 .. 66276135
2622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215710.1

Sequence Viewer

Length: 678 bp
ATGGCAAACACAGCTCTACATCTGGTGCTGATGGTGTTCATCTCCTTGGTGGTGTACTTCACTGAGGCGAAGTGGGCTGAGTCCGTGGAGGCAACAAAGGAAGCGACGCCATCGGAGACAGTAACCAACCTCCAGTTCTACTTCCACGACACAGTCAGTGGGAAGAACCCTAGTGCTGTACGTGTGGCTCAAGCTTCCGACACCGAAAAGTCTCCGACACTTTTCGGGGCTTTGCTCATGGCTGATGACCCGCTCACCGAGACTCCCAACCCCAATTCCAAACTCGTAGGCCGGGCACAAGGGCTATACGGGTCATCCTGCCAGCAGGAATTTGGCCTGATTATGGCCATGAGCTTTGCGTTCTCGGATGGTACGTACAATGGTAGCTCCATTAGTATTTTTGGAAAGAACCCGATCACGAACCCAATTCGGGAGTTGCCGGTTGTGGGTGGAACTGGGGTTTTCCGGTTGGCTCGTGGCTATGCAATTGCCCATACGCATTGGGTAAATGTCACAGCTGGCGATGCAGTTGTTGGATACAATGTCACTAATAGGGACCATATGATCAACAGGCATGAGCCAACTAGAAGTTTCGTGGCCTGCATGTGTATACTTTTGGGTAGTGCTTACGGTAGAAAACAAGCAGATAAGATTGATGCTTCCACAGGTTCCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

225

Amino Acids

24.25

Weight (kDa)

6.29

Isoelectric Point (pI)

25.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 41 - 183 2.3e-55 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 253
AccI GTMKAC 1 cut(s) 610
AciI CCGC 1 cut(s) 251
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 4 cut(s) 56, 180, 373, 377
AfiI CCNNNNNNNGG 3 cut(s) 343, 430, 446
AflIII ACRYGT 1 cut(s) 181
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 5 cut(s) 14, 194, 354, 387, 518
AluI AGCT 5 cut(s) 14, 194, 354, 387, 518
Alw26I GTCTC 3 cut(s) 110, 216, 254
AoxI GGCC 4 cut(s) 289, 334, 345, 597
ApoI RAATTY 1 cut(s) 329
AspS9I GGNCC 1 cut(s) 556
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 247
AvaII GGWCC 1 cut(s) 556
BaeGI GKGCMC 1 cut(s) 298
BalI TGGCCA 1 cut(s) 347
BauI CACGAG 1 cut(s) 474
BccI CCATC 3 cut(s) 25, 118, 362
BciVI GTATCC 1 cut(s) 530
BclI TGATCA 1 cut(s) 564
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 3 cut(s) 110, 216, 254
BfaI CTAG 2 cut(s) 171, 585
BfuI GTATCC 1 cut(s) 530
Bme1390I CCNGG 1 cut(s) 293
Bme18I GGWCC 1 cut(s) 556
BmgT120I GGNCC 1 cut(s) 556
BmiI GGNNCC 2 cut(s) 557, 670
BmrFI CCNGG 1 cut(s) 293
BmrI ACTGGG 1 cut(s) 465
BmsI GCATC 2 cut(s) 514, 646
BmuI ACTGGG 1 cut(s) 465
BpmI CTGGAG 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 293
BsaAI YACGTR 2 cut(s) 182, 375
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 2 cut(s) 45, 84
BsaWI WCCGGW 1 cut(s) 465
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 430, 446
Bse118I RCCGGY 1 cut(s) 439
Bse1I ACTGG 2 cut(s) 133, 460
BseDI CCNNGG 2 cut(s) 45, 84
BseGI GGATG 2 cut(s) 314, 373
BseLI CCNNNNNNNGG 3 cut(s) 343, 430, 446
BseMII CTCAG 2 cut(s) 54, 69
BseNI ACTGG 2 cut(s) 133, 460
BseSI GKGCMC 1 cut(s) 298
BshFI GGCC 4 cut(s) 291, 336, 347, 599
BsiSI CCGG 3 cut(s) 292, 440, 466
BslFI GGGAC 1 cut(s) 569
BslI CCNNNNNNNGG 3 cut(s) 343, 430, 446
BsmAI GTCTC 3 cut(s) 110, 216, 254
BsmFI GGGAC 1 cut(s) 569
BsnI GGCC 4 cut(s) 291, 336, 347, 599
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 2 cut(s) 414, 564
BspACI CCGC 1 cut(s) 251
BspANI GGCC 4 cut(s) 291, 336, 347, 599
BspCNI CTCAG 2 cut(s) 55, 70
BspLI GGNNCC 2 cut(s) 557, 670
BsrBI CCGCTC 1 cut(s) 253
BsrFI RCCGGY 1 cut(s) 439
BsrI ACTGG 2 cut(s) 133, 460
BssAI RCCGGY 1 cut(s) 439
BssECI CCNNGG 2 cut(s) 45, 84
BssMI GATC 2 cut(s) 414, 564
BssNAI GTATAC 1 cut(s) 611
BssNI GRCGYC 1 cut(s) 107
BssSI CACGAG 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 45
Bst1107I GTATAC 1 cut(s) 611
Bst2BI CACGAG 1 cut(s) 474
Bst4CI ACNGT 3 cut(s) 121, 154, 632
BstACI GRCGYC 1 cut(s) 107
BstBAI YACGTR 2 cut(s) 182, 375
BstC8I GCNNGC 3 cut(s) 323, 520, 601
BstDEI CTNAG 2 cut(s) 63, 78
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 2 cut(s) 314, 373
BstKTI GATC 2 cut(s) 417, 567
BstMAI GTCTC 3 cut(s) 110, 216, 254
BstMBI GATC 2 cut(s) 414, 564
BstMWI GCNNNNNNNGC 3 cut(s) 11, 74, 524
BstNSI RCATGY 1 cut(s) 607
BstSCI CCNGG 1 cut(s) 291
BstSLI GKGCMC 1 cut(s) 298
BstSNI TACGTA 1 cut(s) 375
BstZ17I GTATAC 1 cut(s) 611
BsuI GTATCC 1 cut(s) 530
BsuRI GGCC 4 cut(s) 291, 336, 347, 599
BtgI CCRYGG 1 cut(s) 84
BtgZI GCGATG 1 cut(s) 537
BtsCI GGATG 2 cut(s) 314, 373
BtsIMutI CAGTG 2 cut(s) 60, 163
Cac8I GCNNGC 3 cut(s) 323, 520, 601
Cfr10I RCCGGY 1 cut(s) 439
Cfr13I GGNCC 1 cut(s) 556
CseI GACGC 1 cut(s) 115
Csp6I GTAC 4 cut(s) 55, 179, 372, 376
CviAII CATG 4 cut(s) 238, 349, 575, 604
CviQI GTAC 4 cut(s) 55, 179, 372, 376
DdeI CTNAG 2 cut(s) 63, 78
DpnI GATC 2 cut(s) 416, 566
DpnII GATC 2 cut(s) 414, 564
EaeI YGGCCR 1 cut(s) 345
Eco105I TACGTA 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 556
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 4 cut(s) 241, 352, 578, 607
FaqI GGGAC 1 cut(s) 569
FatI CATG 4 cut(s) 237, 348, 574, 603
FauI CCCGC 1 cut(s) 258
FauNDI CATATG 1 cut(s) 561
FbaI TGATCA 1 cut(s) 564
FblI GTMKAC 1 cut(s) 610
FokI GGATG 2 cut(s) 301, 380
FspBI CTAG 2 cut(s) 171, 585
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 4 cut(s) 291, 336, 347, 599
HapII CCGG 3 cut(s) 292, 440, 466
HgaI GACGC 1 cut(s) 115
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 4 cut(s) 241, 352, 578, 607
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 2 cut(s) 80, 262
HpaII CCGG 3 cut(s) 292, 440, 466
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 2 cut(s) 55, 611
Hpy188I TCNGA 4 cut(s) 115, 199, 216, 367
Hpy188III TCNNGA 2 cut(s) 418, 431
Hpy8I GTNNAC 2 cut(s) 55, 611
Hpy99I CGWCG 1 cut(s) 109
HpyCH4III ACNGT 3 cut(s) 121, 154, 632
HpyCH4IV ACGT 2 cut(s) 181, 374
HpyCH4V TGCA 3 cut(s) 485, 527, 603
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 74, 524
HpyF3I CTNAG 2 cut(s) 63, 78
HpySE526I ACGT 2 cut(s) 181, 374
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 4 cut(s) 241, 352, 578, 607
Ksp22I TGATCA 1 cut(s) 564
Kzo9I GATC 2 cut(s) 414, 564
LmnI GCTCC 1 cut(s) 392
LweI GCATC 2 cut(s) 514, 646
MaeI CTAG 2 cut(s) 171, 585
MaeII ACGT 2 cut(s) 181, 374
MaeIII GTNAC 3 cut(s) 121, 511, 544
MalI GATC 2 cut(s) 416, 566
MbiI CCGCTC 1 cut(s) 253
MboI GATC 2 cut(s) 414, 564
MboII GAAGA 1 cut(s) 175
MfeI CAATTG 1 cut(s) 486
MhlI GDGCHC 1 cut(s) 298
MlsI TGGCCA 1 cut(s) 347
MluCI AATT 4 cut(s) 274, 329, 426, 486
MluNI TGGCCA 1 cut(s) 347
MlyI GAGTC 2 cut(s) 89, 256
MmeI TCCRAC 3 cut(s) 222, 239, 514
MnlI CCTC 3 cut(s) 58, 82, 140
Mox20I TGGCCA 1 cut(s) 347
MscI TGGCCA 1 cut(s) 347
Msp20I TGGCCA 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 3 cut(s) 292, 440, 466
MspR9I CCNGG 1 cut(s) 293
MunI CAATTG 1 cut(s) 486
MwoI GCNNNNNNNGC 3 cut(s) 11, 74, 524
NciI CCSGG 1 cut(s) 293
NdeI CATATG 1 cut(s) 561
NdeII GATC 2 cut(s) 414, 564
NlaIII CATG 4 cut(s) 241, 352, 578, 607
NlaIV GGNNCC 2 cut(s) 557, 670
NmuCI GTSAC 2 cut(s) 511, 544
NspI RCATGY 1 cut(s) 607
PcsI WCGNNNNNNNCGW 1 cut(s) 371
PflFI GACNNNGTC 1 cut(s) 152
PleI GAGTC 2 cut(s) 88, 256
PpsI GAGTC 2 cut(s) 88, 256
Ppu21I YACGTR 2 cut(s) 182, 375
PspN4I GGNNCC 2 cut(s) 557, 670
PspPI GGNCC 1 cut(s) 556
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 4 cut(s) 56, 180, 373, 377
RsaNI GTAC 4 cut(s) 55, 179, 372, 376
Sau3AI GATC 2 cut(s) 414, 564
Sau96I GGNCC 1 cut(s) 556
SchI GAGTC 2 cut(s) 89, 256
ScrFI CCNGG 1 cut(s) 293
SduI GDGCHC 1 cut(s) 298
SetI ASST 9 cut(s) 16, 132, 184, 196, 356, 377, 389, 520, 670
SfaNI GCATC 2 cut(s) 514, 646
SinI GGWCC 1 cut(s) 556
SmlI CTYRAG 1 cut(s) 189
SmoI CTYRAG 1 cut(s) 189
SnaBI TACGTA 1 cut(s) 375
Sse9I AATT 4 cut(s) 274, 329, 426, 486
SsiI CCGC 1 cut(s) 251
SspMI CTAG 2 cut(s) 171, 585
StyD4I CCNGG 1 cut(s) 291
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 3 cut(s) 121, 154, 632
TaiI ACGT 2 cut(s) 184, 377
TasI AATT 4 cut(s) 274, 329, 426, 486
TatI WGTACW 1 cut(s) 54
TscAI CASTG 2 cut(s) 67, 163
TseFI GTSAC 2 cut(s) 511, 544
Tsp45I GTSAC 2 cut(s) 511, 544
TspDTI ATGAA 1 cut(s) 28
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 2 cut(s) 67, 163
Tth111I GACNNNGTC 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 556
XapI RAATTY 1 cut(s) 329
XceI RCATGY 1 cut(s) 607
XcmI CCANNNNNNNNNTGG 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 610
XspI CTAG 2 cut(s) 171, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.