Rorug05G0509600

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003188
Physical Location & Seq
Reverse (-)
69294006 .. 69294314
309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0509600.1

Sequence Viewer

Length: 309 bp
ATGGGCGCCCTGGGTTTCATATTGAACATAGAGTTCAAGAATGGTGTTTTTACAATTTCGCCATTAGGATTTAAGACATCGGATAGTCTGTTGTTCAGGAACATGATTGCCGAGCAGTGCTATTACAATTGCCTGCCCTTGATAACCTGTTACGTGGTTTTGATGACTAACCTTATCCGTTCGAGCAAGGATGTGGAGCTCCTATATGGGAAAGGAATAGTGACCTACTCCATGACTGTTGAGTTCTTCAACGAGCTTTGCTCTAATGTCACTGTTAGATTAATTTGGACTTATCACAAGTTATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

102

Amino Acids

11.67

Weight (kDa)

7.64

Isoelectric Point (pI)

27.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 7 - 92 2.2e-18 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 5
AcyI GRCGYC 1 cut(s) 6
AgsI TTSAA 3 cut(s) 25, 37, 250
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 2 cut(s) 199, 256
AluI AGCT 2 cut(s) 199, 256
Alw21I GWGCWC 1 cut(s) 201
AseI ATTAAT 1 cut(s) 281
AspLEI GCGC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 201
BciT130I CCWGG 1 cut(s) 11
BfaI CTAG 1 cut(s) 307
BfoI RGCGCY 1 cut(s) 9
Bme1390I CCNGG 1 cut(s) 11
BmiI GGNNCC 1 cut(s) 7
BmrFI CCNGG 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 245, 277
BsaAI YACGTR 1 cut(s) 154
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 2 cut(s) 9, 10
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 2 cut(s) 9, 10
BseGI GGATG 1 cut(s) 196
BshNI GGYRCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 7
BspT107I GGYRCC 1 cut(s) 5
BssECI CCNNGG 2 cut(s) 9, 10
BssNI GRCGYC 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 238, 274
BstACI GRCGYC 1 cut(s) 6
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 1 cut(s) 134
BstF5I GGATG 1 cut(s) 196
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 1 cut(s) 8
BstNI CCWGG 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 9
BtsCI GGATG 1 cut(s) 196
BtsI GCAGTG 1 cut(s) 122
BtsIMutI CAGTG 2 cut(s) 122, 270
Cac8I GCNNGC 1 cut(s) 134
CfoI GCGC 1 cut(s) 8
CviAII CATG 2 cut(s) 103, 232
CviJI RGCY 2 cut(s) 199, 256
CviKI_1 RGCY 2 cut(s) 199, 256
DinI GGCGCC 1 cut(s) 7
Ecl136II GAGCTC 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 199
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 201
EgeI GGCGCC 1 cut(s) 7
EheI GGCGCC 1 cut(s) 7
FaeI CATG 2 cut(s) 106, 235
FaiI YATR 6 cut(s) 20, 29, 104, 205, 207, 233
FatI CATG 2 cut(s) 102, 231
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 1 cut(s) 201
FspBI CTAG 1 cut(s) 307
GlaI GCGC 1 cut(s) 7
HaeII RGCGCY 1 cut(s) 9
HhaI GCGC 1 cut(s) 8
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 2 cut(s) 106, 235
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 2 cut(s) 37, 97
HpyCH4III ACNGT 2 cut(s) 238, 274
HpyCH4IV ACGT 1 cut(s) 153
HpySE526I ACGT 1 cut(s) 153
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 2 cut(s) 106, 235
HspAI GCGC 1 cut(s) 6
KasI GGCGCC 1 cut(s) 5
LmnI GCTCC 2 cut(s) 196, 204
LpnPI CCDG 4 cut(s) 23, 82, 146, 160
MaeI CTAG 1 cut(s) 307
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 3 cut(s) 149, 220, 268
MboII GAAGA 1 cut(s) 238
MfeI CAATTG 1 cut(s) 127
MhlI GDGCHC 1 cut(s) 201
MluCI AATT 3 cut(s) 54, 127, 282
Mly113I GGCGCC 1 cut(s) 6
MseI TTAA 2 cut(s) 72, 281
MspR9I CCNGG 1 cut(s) 11
MunI CAATTG 1 cut(s) 127
MvaI CCWGG 1 cut(s) 11
NarI GGCGCC 1 cut(s) 6
NlaIII CATG 2 cut(s) 106, 235
NlaIV GGNNCC 1 cut(s) 7
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 2 cut(s) 220, 268
PasI CCCWGGG 1 cut(s) 10
PluTI GGCGCC 1 cut(s) 9
Ppu21I YACGTR 1 cut(s) 154
PshBI ATTAAT 1 cut(s) 281
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 7
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 2 cut(s) 72, 281
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 201
SetI ASST 6 cut(s) 149, 156, 174, 201, 227, 258
SfoI GGCGCC 1 cut(s) 7
Sse9I AATT 3 cut(s) 54, 127, 282
SspDI GGCGCC 1 cut(s) 5
SspMI CTAG 1 cut(s) 307
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 9
TaaI ACNGT 2 cut(s) 238, 274
TaiI ACGT 1 cut(s) 156
TaqI TCGA 1 cut(s) 182
TasI AATT 3 cut(s) 54, 127, 282
Tru1I TTAA 2 cut(s) 72, 281
Tru9I TTAA 2 cut(s) 72, 281
TscAI CASTG 2 cut(s) 122, 277
TseFI GTSAC 2 cut(s) 220, 268
Tsp45I GTSAC 2 cut(s) 220, 268
TspDTI ATGAA 1 cut(s) 7
TspGWI ACGGA 1 cut(s) 167
TspRI CASTG 2 cut(s) 122, 277
VspI ATTAAT 1 cut(s) 281
XspI CTAG 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.