Rh6AG025300

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003188
Physical Location & Seq
Reverse (-)
2915959 .. 2916902
944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG025300.1

Sequence Viewer

Length: 702 bp
ATGCATTATACTTCCCAAGTCCTGCGGTGGGTAGTTCAACAACATCGAATCCTCGGCCCTTATAATCTGCAGACCGAAACATCCACACATGCATCTCCTTTTTGCTTGGACAAACAAGACACGACAGAAATTAAGCAGAAAATGGCAAACAACACAGTTGCGCCTCTGATGATGATGGTCGTCATCTCCTTGGTGATGGCATTCACAGAAGCCAAGTGGGCCAAGTCAGTAGAGGCCAAAAAGGAAGCGACGCCGTCGGAGACAGTAACCAACCTCCAGTTCTTCTTCCACGACACAGTCAGTGGGAAGAACCCTAGTGCCGTACGTGTGGCCCAAGCCTCAGACACCAATAAGTCTCTGACACTTTTCGGGGCTTTACTCATGGCCGATGACCCGCTCACCGAGACTCCCGACCCCAATTCCAAGCTAGTGGGTCGGGCACAAGGGCTATACGGGTCATCCGGCCAGCAGGAGCTTGGCCTGATTATGGCTATGAGCTTTGCGTTCACGGATGGTCCTTACAGTGGTAGTTCCATTAGTATATTTGGTAAAAACCCGGCACTGAACCCAGTTCGAGAGTTGCCGGTTGTGGGTGGTACTGGGGTGTTCCGGCTGGCTCGTGGATATGCAATTGCCCATACTCATTGGTTTGATCTTAGTGGTGATGCAATTGTAGGATACAATGTCACTGTGATTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

233

Amino Acids

25.35

Weight (kDa)

6.65

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 89 - 231 2.5e-54 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 63
AccBSI CCGCTC 1 cut(s) 397
AciI CCGC 2 cut(s) 25, 395
AcoI YGGCCR 2 cut(s) 384, 463
AcyI GRCGYC 1 cut(s) 251
AfaI GTAC 2 cut(s) 324, 598
AfiI CCNNNNNNNGG 4 cut(s) 28, 487, 524, 590
AflIII ACRYGT 1 cut(s) 325
AgsI TTSAA 1 cut(s) 38
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 3 cut(s) 427, 475, 498
AluI AGCT 3 cut(s) 427, 475, 498
Alw26I GTCTC 3 cut(s) 254, 360, 398
AoxI GGCC 7 cut(s) 55, 219, 234, 330, 384, 463, 478
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 4 cut(s) 56, 219, 331, 515
AsuC2I CCSGG 1 cut(s) 557
AsuHPI GGTGA 3 cut(s) 205, 391, 674
AvaII GGWCC 1 cut(s) 515
BaeGI GKGCMC 1 cut(s) 442
BaeI ACNNNNGTAYC 2 cut(s) 588, 621
BauI CACGAG 1 cut(s) 618
BccI CCATC 3 cut(s) 169, 190, 506
BceAI ACGGC 2 cut(s) 238, 305
BciVI GTATCC 1 cut(s) 671
BcnI CCSGG 1 cut(s) 557
BcoDI GTCTC 3 cut(s) 254, 360, 398
BfaI CTAG 3 cut(s) 315, 428, 700
BfmI CTRYAG 1 cut(s) 68
BfuI GTATCC 1 cut(s) 671
BglI GCCNNNNNGGC 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 557
Bme18I GGWCC 1 cut(s) 515
BmgT120I GGNCC 4 cut(s) 56, 219, 331, 515
BmrFI CCNGG 1 cut(s) 557
BmrI ACTGGG 2 cut(s) 563, 609
BmsI GCATC 2 cut(s) 101, 655
BmuI ACTGGG 2 cut(s) 563, 609
BpmI CTGGAG 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 557
BsaAI YACGTR 1 cut(s) 326
BsaHI GRCGYC 1 cut(s) 251
BsaJI CCNNGG 2 cut(s) 52, 189
BsaXI ACNNNNNCTCC 2 cut(s) 391, 421
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 487, 524, 590
Bse118I RCCGGY 1 cut(s) 583
Bse1I ACTGG 3 cut(s) 277, 569, 604
BseDI CCNNGG 2 cut(s) 52, 189
BseGI GGATG 3 cut(s) 80, 458, 517
BseLI CCNNNNNNNGG 4 cut(s) 28, 487, 524, 590
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 3 cut(s) 277, 569, 604
BseSI GKGCMC 1 cut(s) 442
BshFI GGCC 7 cut(s) 57, 221, 236, 332, 386, 465, 480
BsiSI CCGG 4 cut(s) 462, 557, 584, 610
BsiWI CGTACG 1 cut(s) 322
BslI CCNNNNNNNGG 4 cut(s) 28, 487, 524, 590
BsmAI GTCTC 3 cut(s) 254, 360, 398
BsmI GAATGC 1 cut(s) 200
BsnI GGCC 7 cut(s) 57, 221, 236, 332, 386, 465, 480
Bsp1286I GDGCHC 1 cut(s) 442
Bsp143I GATC 1 cut(s) 652
BspACI CCGC 2 cut(s) 25, 395
BspANI GGCC 7 cut(s) 57, 221, 236, 332, 386, 465, 480
BspCNI CTCAG 1 cut(s) 353
BspMAI CTGCAG 1 cut(s) 72
BsrBI CCGCTC 1 cut(s) 397
BsrFI RCCGGY 1 cut(s) 583
BsrI ACTGG 3 cut(s) 277, 569, 604
BssAI RCCGGY 1 cut(s) 583
BssECI CCNNGG 2 cut(s) 52, 189
BssMI GATC 1 cut(s) 652
BssNI GRCGYC 1 cut(s) 251
BssSI CACGAG 1 cut(s) 618
BssT1I CCWWGG 1 cut(s) 189
Bst2BI CACGAG 1 cut(s) 618
Bst4CI ACNGT 5 cut(s) 157, 265, 298, 524, 691
BstACI GRCGYC 1 cut(s) 251
BstBAI YACGTR 1 cut(s) 326
BstC8I GCNNGC 2 cut(s) 467, 615
BstDEI CTNAG 2 cut(s) 340, 656
BstF5I GGATG 3 cut(s) 80, 458, 517
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 1 cut(s) 655
BstMAI GTCTC 3 cut(s) 254, 360, 398
BstMBI GATC 1 cut(s) 652
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstNSI RCATGY 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 555
BstSFI CTRYAG 1 cut(s) 68
BstSLI GKGCMC 1 cut(s) 442
BstXI CCANNNNNNTGG 1 cut(s) 430
BsuI GTATCC 1 cut(s) 671
BsuRI GGCC 7 cut(s) 57, 221, 236, 332, 386, 465, 480
BtsCI GGATG 3 cut(s) 80, 458, 517
BtsIMutI CAGTG 4 cut(s) 307, 529, 560, 687
Cac8I GCNNGC 2 cut(s) 467, 615
CfoI GCGC 1 cut(s) 163
Cfr10I RCCGGY 1 cut(s) 583
Cfr13I GGNCC 4 cut(s) 56, 219, 331, 515
CseI GACGC 1 cut(s) 259
Csp6I GTAC 2 cut(s) 323, 597
CviAII CATG 2 cut(s) 89, 382
CviQI GTAC 2 cut(s) 323, 597
DdeI CTNAG 2 cut(s) 340, 656
DpnI GATC 1 cut(s) 654
DpnII GATC 1 cut(s) 652
EaeI YGGCCR 2 cut(s) 384, 463
Eco130I CCWWGG 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 515
EcoT14I CCWWGG 1 cut(s) 189
EcoT22I ATGCAT 2 cut(s) 6, 94
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 2 cut(s) 92, 385
FatI CATG 2 cut(s) 88, 381
FauI CCCGC 1 cut(s) 402
FokI GGATG 3 cut(s) 67, 445, 524
FspBI CTAG 3 cut(s) 315, 428, 700
GlaI GCGC 1 cut(s) 162
GsuI CTGGAG 1 cut(s) 260
HaeIII GGCC 7 cut(s) 57, 221, 236, 332, 386, 465, 480
HapII CCGG 4 cut(s) 462, 557, 584, 610
HgaI GACGC 1 cut(s) 259
HhaI GCGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 251
Hin1II CATG 2 cut(s) 92, 385
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 3 cut(s) 48, 406, 694
HpaII CCGG 4 cut(s) 462, 557, 584, 610
HphI GGTGA 3 cut(s) 205, 391, 674
Hpy166II GTNNAC 1 cut(s) 507
Hpy188I TCNGA 4 cut(s) 168, 259, 343, 360
Hpy188III TCNNGA 2 cut(s) 410, 575
Hpy8I GTNNAC 1 cut(s) 507
Hpy99I CGWCG 2 cut(s) 253, 259
HpyCH4III ACNGT 5 cut(s) 157, 265, 298, 524, 691
HpyCH4IV ACGT 1 cut(s) 325
HpyCH4V TGCA 5 cut(s) 4, 70, 92, 629, 668
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 2 cut(s) 340, 656
HpySE526I ACGT 1 cut(s) 325
Hsp92I GRCGYC 1 cut(s) 251
Hsp92II CATG 2 cut(s) 92, 385
HspAI GCGC 1 cut(s) 161
Kzo9I GATC 1 cut(s) 652
LmnI GCTCC 1 cut(s) 472
LweI GCATC 2 cut(s) 101, 655
MaeI CTAG 3 cut(s) 315, 428, 700
MaeII ACGT 1 cut(s) 325
MaeIII GTNAC 2 cut(s) 265, 685
MalI GATC 1 cut(s) 654
MbiI CCGCTC 1 cut(s) 397
MboI GATC 1 cut(s) 652
MboII GAAGA 3 cut(s) 274, 277, 319
MfeI CAATTG 2 cut(s) 630, 669
MhlI GDGCHC 1 cut(s) 442
MluCI AATT 4 cut(s) 129, 418, 630, 669
MlyI GAGTC 1 cut(s) 400
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 5 cut(s) 62, 174, 226, 284, 349
Mph1103I ATGCAT 2 cut(s) 6, 94
MseI TTAA 1 cut(s) 132
MspI CCGG 4 cut(s) 462, 557, 584, 610
MspR9I CCNGG 1 cut(s) 557
MunI CAATTG 2 cut(s) 630, 669
Mva1269I GAATGC 1 cut(s) 200
MwoI GCNNNNNNNGC 1 cut(s) 218
NciI CCSGG 1 cut(s) 557
NdeII GATC 1 cut(s) 652
NlaIII CATG 2 cut(s) 92, 385
NmeAIII GCCGAG 1 cut(s) 33
NmuCI GTSAC 1 cut(s) 685
NsiI ATGCAT 2 cut(s) 6, 94
NspI RCATGY 1 cut(s) 92
PctI GAATGC 1 cut(s) 200
PfeI GAWTC 2 cut(s) 48, 694
Pfl23II CGTACG 1 cut(s) 322
PflFI GACNNNGTC 2 cut(s) 253, 296
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
Ppu21I YACGTR 1 cut(s) 326
PsiI TTATAA 1 cut(s) 63
PspLI CGTACG 1 cut(s) 322
PspPI GGNCC 4 cut(s) 56, 219, 331, 515
PstI CTGCAG 1 cut(s) 72
PsyI GACNNNGTC 2 cut(s) 253, 296
RsaI GTAC 2 cut(s) 324, 598
RsaNI GTAC 2 cut(s) 323, 597
SaqAI TTAA 1 cut(s) 132
Sau3AI GATC 1 cut(s) 652
Sau96I GGNCC 4 cut(s) 56, 219, 331, 515
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 1 cut(s) 557
SduI GDGCHC 1 cut(s) 442
SetI ASST 5 cut(s) 276, 328, 429, 477, 500
SfaNI GCATC 2 cut(s) 101, 655
SfcI CTRYAG 1 cut(s) 68
SinI GGWCC 1 cut(s) 515
Sse9I AATT 4 cut(s) 129, 418, 630, 669
SsiI CCGC 2 cut(s) 25, 395
SspMI CTAG 3 cut(s) 315, 428, 700
StyD4I CCNGG 1 cut(s) 555
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 5 cut(s) 157, 265, 298, 524, 691
TaiI ACGT 1 cut(s) 328
TaqI TCGA 2 cut(s) 46, 574
TaqII GACCGA 1 cut(s) 89
TasI AATT 4 cut(s) 129, 418, 630, 669
TfiI GAWTC 2 cut(s) 48, 694
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TscAI CASTG 4 cut(s) 307, 529, 567, 694
TseFI GTSAC 1 cut(s) 685
Tsp45I GTSAC 1 cut(s) 685
TspGWI ACGGA 1 cut(s) 524
TspRI CASTG 4 cut(s) 307, 529, 567, 694
Tth111I GACNNNGTC 2 cut(s) 253, 296
VpaK11BI GGWCC 1 cut(s) 515
XceI RCATGY 1 cut(s) 92
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
XspI CTAG 3 cut(s) 315, 428, 700
Zsp2I ATGCAT 2 cut(s) 6, 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.