Rw6G002070

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003188
Physical Location & Seq
Reverse (-)
3285564 .. 3286198
635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002070.1

Sequence Viewer

Length: 564 bp
ATGGCAAACACAGCTCTGCCGCTAATGATGTTAATGATCACCTCCTTGTTGATGGCCTTGACGGAAGCTTCGTGGGCAGAGTCAGTCGAAGCAAAAAAGGAGGCCACGCCTTTGGAGACCATAACCAACCTCCAGTTCTACTTCCACGACACAGTCAGTGGGAAGAACCCCAGTGCCGTACGGGTGGCACAAGCCGCCAACACCGACAAGTCCCCAACCCTCTTCGGGGTTCTGCTTATGGCCGATGACCCCCTTACTGAGACGCCGGACCCTAATTCCAAGCTCGTGGGACGGGCGCAAGGGCTGTACGGGTCATCCGGCCAGCAGGAATTTGGCCTCATTATGGCCATGAGCTTTGCGTTCACAGATGGTACGTTCAATGGTAGCTCCATCAGTATTTTTGGTAAAAACCCAGCTCTACACCCGGTTCGGGAGTTGCCGGTTGTGGGTGGAACCGGGGTGTTCCGATTGGCTCGTGGATATGCAATTGCCAAGACTTATCGGCTTGATAGCACTGGTGATGCAATTGTAGGATACAATGTCACTGTAACTCATCAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

187

Amino Acids

19.94

Weight (kDa)

5.55

Isoelectric Point (pI)

25.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 42 - 183 6.8e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 20, 195
AcoI YGGCCR 3 cut(s) 240, 319, 345
AcsI RAATTY 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 263
AfaI GTAC 3 cut(s) 180, 308, 373
AfiI CCNNNNNNNGG 5 cut(s) 225, 226, 343, 430, 446
AgsI TTSAA 1 cut(s) 379
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 7 cut(s) 14, 68, 283, 354, 387, 416, 561
AluI AGCT 7 cut(s) 14, 68, 283, 354, 387, 416, 561
Alw26I GTCTC 2 cut(s) 110, 254
AoxI GGCC 6 cut(s) 54, 102, 240, 319, 334, 345
ApoI RAATTY 1 cut(s) 329
ArsI GACNNNNNNTTYG 2 cut(s) 52, 84
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 1 cut(s) 268
AsuC2I CCSGG 2 cut(s) 425, 457
AsuHPI GGTGA 2 cut(s) 31, 530
AvaII GGWCC 1 cut(s) 268
BalI TGGCCA 1 cut(s) 347
BauI CACGAG 2 cut(s) 284, 474
BccI CCATC 3 cut(s) 46, 362, 398
BceAI ACGGC 1 cut(s) 161
BciVI GTATCC 1 cut(s) 527
BclI TGATCA 1 cut(s) 36
BcnI CCSGG 2 cut(s) 425, 457
BcoDI GTCTC 2 cut(s) 110, 254
BfaI CTAG 1 cut(s) 562
BfuI GTATCC 1 cut(s) 527
BisI GCNGC 2 cut(s) 20, 195
BlsI GCNGC 2 cut(s) 21, 196
Bme1390I CCNGG 2 cut(s) 425, 457
Bme18I GGWCC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 2 cut(s) 270, 454
BmrFI CCNGG 2 cut(s) 425, 457
BmrI ACTGGG 1 cut(s) 165
BmsI GCATC 1 cut(s) 511
BmuI ACTGGG 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 116
BpuMI CCSGG 2 cut(s) 425, 457
BsaHI GRCGYC 1 cut(s) 263
BsaI GGTCTC 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 456
Bsc4I CCNNNNNNNGG 5 cut(s) 225, 226, 343, 430, 446
Bse118I RCCGGY 1 cut(s) 439
Bse1I ACTGG 3 cut(s) 133, 171, 520
BseDI CCNNGG 1 cut(s) 456
BseGI GGATG 1 cut(s) 314
BseLI CCNNNNNNNGG 5 cut(s) 225, 226, 343, 430, 446
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 3 cut(s) 133, 171, 520
BseYI CCCAGC 1 cut(s) 412
BshFI GGCC 6 cut(s) 56, 104, 242, 321, 336, 347
BsiSI CCGG 5 cut(s) 266, 318, 425, 440, 456
BsiWI CGTACG 1 cut(s) 178
BslFI GGGAC 2 cut(s) 196, 303
BslI CCNNNNNNNGG 5 cut(s) 225, 226, 343, 430, 446
BsmAI GTCTC 2 cut(s) 110, 254
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 2 cut(s) 196, 303
BsnI GGCC 6 cut(s) 56, 104, 242, 321, 336, 347
Bso31I GGTCTC 1 cut(s) 110
Bsp143I GATC 1 cut(s) 36
BspACI CCGC 2 cut(s) 20, 195
BspANI GGCC 6 cut(s) 56, 104, 242, 321, 336, 347
BspCNI CTCAG 1 cut(s) 250
BspLI GGNNCC 2 cut(s) 270, 454
BspTNI GGTCTC 1 cut(s) 110
BsrFI RCCGGY 1 cut(s) 439
BsrI ACTGG 3 cut(s) 133, 171, 520
BssAI RCCGGY 1 cut(s) 439
BssECI CCNNGG 1 cut(s) 456
BssMI GATC 1 cut(s) 36
BssNI GRCGYC 1 cut(s) 263
BssSI CACGAG 2 cut(s) 284, 474
Bst2BI CACGAG 2 cut(s) 284, 474
Bst4CI ACNGT 2 cut(s) 154, 547
Bst6I CTCTTC 1 cut(s) 227
BstACI GRCGYC 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 323
BstDEI CTNAG 1 cut(s) 258
BstF5I GGATG 1 cut(s) 314
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 1 cut(s) 39
BstMAI GTCTC 2 cut(s) 110, 254
BstMBI GATC 1 cut(s) 36
BstMWI GCNNNNNNNGC 3 cut(s) 11, 74, 194
BstSCI CCNGG 2 cut(s) 423, 455
BstXI CCANNNNNNTGG 2 cut(s) 112, 286
BsuI GTATCC 1 cut(s) 527
BsuRI GGCC 6 cut(s) 56, 104, 242, 321, 336, 347
BtsCI GGATG 1 cut(s) 314
BtsIMutI CAGTG 4 cut(s) 163, 178, 513, 543
Cac8I GCNNGC 1 cut(s) 323
CfoI GCGC 1 cut(s) 298
Cfr10I RCCGGY 1 cut(s) 439
Cfr13I GGNCC 1 cut(s) 268
CseI GACGC 1 cut(s) 271
Csp6I GTAC 3 cut(s) 179, 307, 372
CviAII CATG 1 cut(s) 349
CviQI GTAC 3 cut(s) 179, 307, 372
DdeI CTNAG 1 cut(s) 258
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
EaeI YGGCCR 3 cut(s) 240, 319, 345
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco31I GGTCTC 1 cut(s) 110
Eco47I GGWCC 1 cut(s) 268
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 1 cut(s) 352
FaiI YATR 5 cut(s) 122, 239, 344, 350, 483
FaqI GGGAC 2 cut(s) 196, 303
FatI CATG 1 cut(s) 348
FbaI TGATCA 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 20, 195
FokI GGATG 1 cut(s) 301
Fsp4HI GCNGC 2 cut(s) 20, 195
FspBI CTAG 1 cut(s) 562
GlaI GCGC 1 cut(s) 297
GluI GCNGC 2 cut(s) 20, 195
GsaI CCCAGC 1 cut(s) 416
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 6 cut(s) 56, 104, 242, 321, 336, 347
HapII CCGG 5 cut(s) 266, 318, 425, 440, 456
HgaI GACGC 1 cut(s) 271
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 1 cut(s) 352
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HindIII AAGCTT 1 cut(s) 66
HinfI GANTC 1 cut(s) 80
HpaII CCGG 5 cut(s) 266, 318, 425, 440, 456
HphI GGTGA 2 cut(s) 31, 530
Hpy166II GTNNAC 1 cut(s) 363
Hpy188I TCNGA 2 cut(s) 467, 558
Hpy188III TCNNGA 1 cut(s) 431
Hpy8I GTNNAC 1 cut(s) 363
HpyCH4III ACNGT 2 cut(s) 154, 547
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 2 cut(s) 485, 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 74, 194
HpyF3I CTNAG 1 cut(s) 258
HpySE526I ACGT 1 cut(s) 374
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 1 cut(s) 352
HspAI GCGC 1 cut(s) 296
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 1 cut(s) 36
LmnI GCTCC 1 cut(s) 392
LweI GCATC 1 cut(s) 511
MaeI CTAG 1 cut(s) 562
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 2 cut(s) 541, 547
MalI GATC 1 cut(s) 38
MboI GATC 1 cut(s) 36
MboII GAAGA 2 cut(s) 175, 214
MfeI CAATTG 2 cut(s) 486, 525
MlsI TGGCCA 1 cut(s) 347
MluCI AATT 4 cut(s) 274, 329, 486, 525
MluNI TGGCCA 1 cut(s) 347
MlyI GAGTC 1 cut(s) 89
MnlI CCTC 5 cut(s) 52, 94, 140, 230, 347
Mox20I TGGCCA 1 cut(s) 347
MscI TGGCCA 1 cut(s) 347
MseI TTAA 1 cut(s) 32
Msp20I TGGCCA 1 cut(s) 347
MspI CCGG 5 cut(s) 266, 318, 425, 440, 456
MspR9I CCNGG 2 cut(s) 425, 457
MunI CAATTG 2 cut(s) 486, 525
MwoI GCNNNNNNNGC 3 cut(s) 11, 74, 194
NciI CCSGG 2 cut(s) 425, 457
NdeII GATC 1 cut(s) 36
NlaIII CATG 1 cut(s) 352
NlaIV GGNNCC 2 cut(s) 270, 454
NmuCI GTSAC 1 cut(s) 541
PcsI WCGNNNNNNNCGW 1 cut(s) 68
Pfl23II CGTACG 1 cut(s) 178
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 2 cut(s) 21, 196
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PspFI CCCAGC 1 cut(s) 412
PspLI CGTACG 1 cut(s) 178
PspN4I GGNNCC 2 cut(s) 270, 454
PspPI GGNCC 1 cut(s) 268
PsyI GACNNNGTC 1 cut(s) 152
RsaI GTAC 3 cut(s) 180, 308, 373
RsaNI GTAC 3 cut(s) 179, 307, 372
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 2 cut(s) 20, 195
Sau3AI GATC 1 cut(s) 36
Sau96I GGNCC 1 cut(s) 268
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 425, 457
SfaNI GCATC 1 cut(s) 511
SinI GGWCC 1 cut(s) 268
Sse9I AATT 4 cut(s) 274, 329, 486, 525
SsiI CCGC 2 cut(s) 20, 195
SspMI CTAG 1 cut(s) 562
StyD4I CCNGG 2 cut(s) 423, 455
TaaI ACNGT 2 cut(s) 154, 547
TaiI ACGT 1 cut(s) 377
TaqI TCGA 1 cut(s) 87
TasI AATT 4 cut(s) 274, 329, 486, 525
TauI GCSGC 2 cut(s) 22, 197
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 4 cut(s) 163, 178, 520, 550
TseFI GTSAC 1 cut(s) 541
Tsp45I GTSAC 1 cut(s) 541
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 4 cut(s) 163, 178, 520, 550
Tth111I GACNNNGTC 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 268
XapI RAATTY 1 cut(s) 329
XcmI CCANNNNNNNNNTGG 1 cut(s) 329
XspI CTAG 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.