Rh6DG019600

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003188
Physical Location & Seq
Reverse (-)
1763382 .. 1763945
564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG019600.1

Sequence Viewer

Length: 564 bp
ATGGCAAACACAGCTCTGCCGCTAATGATGTTAATGATCACCTCCTTGCTGATGGCCTTGACGGAAGCTGAGTGGGCAGAGTCAGTTGAAGCAAAAAAGGAGGCCACGCCTTTGGAGACCGTAACCAACCTCCAGTTCTACTTCCACGACACAGTCAGTGGGAAGAACCCCAGTGCCGTACGTGTGGCACAAGCCGCCAACACCGACAAGTCCCCAACCCTCTTTGGGGCTCTGCTCATGGCCGATGACCCGCTTACTGAGACGCCGGACCCTAATTCCAAGCTCGTGGGACGGGCGCAAGGGCTGTATGGGTCATCCGGCCAGCAGGAATTTGGCCTCATTATGGCCATGAGCTTTGCGTTCACGGATGGTGCGTTCAATGGTAGCTCCGTTAGTATTTTTGGTAAAAACCCAGCTCTACACCCGGTTCGGGAGTTGCCGGTTGTGGGTGGAACTGGGGTGTTCCGATTGGCTCGTGGATATGCAATTGCCAAGACTTATCGGCTTGATACCACTGGTGATGCAATTGTAGGATACAATGTCACTGTAACTCACCAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

187

Amino Acids

19.91

Weight (kDa)

5.3

Isoelectric Point (pI)

20.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 41 - 183 5.9e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 20, 195, 251
AcoI YGGCCR 3 cut(s) 240, 319, 345
AcsI RAATTY 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 263
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 5 cut(s) 225, 226, 343, 430, 446
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 2 cut(s) 89, 379
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AloI GAACNNNNNNTCC 2 cut(s) 359, 391
AluBI AGCT 7 cut(s) 14, 68, 283, 354, 387, 416, 561
AluI AGCT 7 cut(s) 14, 68, 283, 354, 387, 416, 561
Alw26I GTCTC 2 cut(s) 110, 254
AoxI GGCC 6 cut(s) 54, 102, 240, 319, 334, 345
ApoI RAATTY 1 cut(s) 329
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 1 cut(s) 268
AsuC2I CCSGG 1 cut(s) 425
AsuHPI GGTGA 3 cut(s) 31, 530, 545
AvaII GGWCC 1 cut(s) 268
BalI TGGCCA 1 cut(s) 347
BanII GRGCYC 1 cut(s) 232
BauI CACGAG 2 cut(s) 284, 474
BccI CCATC 2 cut(s) 46, 362
BceAI ACGGC 1 cut(s) 161
BciVI GTATCC 1 cut(s) 527
BclI TGATCA 1 cut(s) 36
BcnI CCSGG 1 cut(s) 425
BcoDI GTCTC 2 cut(s) 110, 254
BfaI CTAG 1 cut(s) 562
BfuI GTATCC 1 cut(s) 527
BisI GCNGC 2 cut(s) 20, 195
BlsI GCNGC 2 cut(s) 21, 196
Bme1390I CCNGG 1 cut(s) 425
Bme18I GGWCC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 1 cut(s) 270
BmrFI CCNGG 1 cut(s) 425
BmrI ACTGGG 2 cut(s) 165, 465
BmsI GCATC 1 cut(s) 511
BmuI ACTGGG 2 cut(s) 165, 465
BpmI CTGGAG 1 cut(s) 116
BpuMI CCSGG 1 cut(s) 425
BsaAI YACGTR 1 cut(s) 182
BsaHI GRCGYC 1 cut(s) 263
BsaI GGTCTC 1 cut(s) 110
Bsc4I CCNNNNNNNGG 5 cut(s) 225, 226, 343, 430, 446
Bse118I RCCGGY 1 cut(s) 439
Bse1I ACTGG 4 cut(s) 133, 171, 460, 520
BseGI GGATG 2 cut(s) 314, 373
BseLI CCNNNNNNNGG 5 cut(s) 225, 226, 343, 430, 446
BseMII CTCAG 2 cut(s) 60, 249
BseNI ACTGG 4 cut(s) 133, 171, 460, 520
BseYI CCCAGC 1 cut(s) 412
BshFI GGCC 6 cut(s) 56, 104, 242, 321, 336, 347
BsiSI CCGG 4 cut(s) 266, 318, 425, 440
BsiWI CGTACG 1 cut(s) 178
BslFI GGGAC 2 cut(s) 196, 303
BslI CCNNNNNNNGG 5 cut(s) 225, 226, 343, 430, 446
BsmAI GTCTC 2 cut(s) 110, 254
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 2 cut(s) 196, 303
BsnI GGCC 6 cut(s) 56, 104, 242, 321, 336, 347
Bso31I GGTCTC 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 232
Bsp143I GATC 1 cut(s) 36
BspACI CCGC 3 cut(s) 20, 195, 251
BspANI GGCC 6 cut(s) 56, 104, 242, 321, 336, 347
BspCNI CTCAG 2 cut(s) 61, 250
BspLI GGNNCC 1 cut(s) 270
BspTNI GGTCTC 1 cut(s) 110
BsrFI RCCGGY 1 cut(s) 439
BsrI ACTGG 4 cut(s) 133, 171, 460, 520
BssAI RCCGGY 1 cut(s) 439
BssMI GATC 1 cut(s) 36
BssNI GRCGYC 1 cut(s) 263
BssSI CACGAG 2 cut(s) 284, 474
Bst2BI CACGAG 2 cut(s) 284, 474
Bst4CI ACNGT 3 cut(s) 121, 154, 547
BstACI GRCGYC 1 cut(s) 263
BstBAI YACGTR 1 cut(s) 182
BstC8I GCNNGC 1 cut(s) 323
BstDEI CTNAG 2 cut(s) 69, 258
BstF5I GGATG 2 cut(s) 314, 373
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 1 cut(s) 39
BstMAI GTCTC 2 cut(s) 110, 254
BstMBI GATC 1 cut(s) 36
BstMWI GCNNNNNNNGC 3 cut(s) 11, 74, 194
BstSCI CCNGG 1 cut(s) 423
BstXI CCANNNNNNTGG 2 cut(s) 112, 286
BsuI GTATCC 1 cut(s) 527
BsuRI GGCC 6 cut(s) 56, 104, 242, 321, 336, 347
BtsCI GGATG 2 cut(s) 314, 373
BtsIMutI CAGTG 4 cut(s) 163, 178, 513, 543
Cac8I GCNNGC 1 cut(s) 323
CfoI GCGC 1 cut(s) 298
Cfr10I RCCGGY 1 cut(s) 439
Cfr13I GGNCC 1 cut(s) 268
CseI GACGC 1 cut(s) 271
Csp6I GTAC 1 cut(s) 179
CviAII CATG 2 cut(s) 238, 349
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 2 cut(s) 69, 258
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
EaeI YGGCCR 3 cut(s) 240, 319, 345
Eco24I GRGCYC 1 cut(s) 232
Eco31I GGTCTC 1 cut(s) 110
Eco47I GGWCC 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 232
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 2 cut(s) 241, 352
FaiI YATR 5 cut(s) 239, 309, 344, 350, 483
FaqI GGGAC 2 cut(s) 196, 303
FatI CATG 2 cut(s) 237, 348
FauI CCCGC 1 cut(s) 258
FbaI TGATCA 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 20, 195
FokI GGATG 2 cut(s) 301, 380
FriOI GRGCYC 1 cut(s) 232
Fsp4HI GCNGC 2 cut(s) 20, 195
FspBI CTAG 1 cut(s) 562
GlaI GCGC 1 cut(s) 297
GluI GCNGC 2 cut(s) 20, 195
GsaI CCCAGC 1 cut(s) 416
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 6 cut(s) 56, 104, 242, 321, 336, 347
HapII CCGG 4 cut(s) 266, 318, 425, 440
HgaI GACGC 1 cut(s) 271
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 2 cut(s) 241, 352
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HinfI GANTC 1 cut(s) 80
HpaII CCGG 4 cut(s) 266, 318, 425, 440
HphI GGTGA 3 cut(s) 31, 530, 545
Hpy166II GTNNAC 1 cut(s) 363
Hpy188I TCNGA 1 cut(s) 467
Hpy188III TCNNGA 1 cut(s) 431
Hpy8I GTNNAC 1 cut(s) 363
HpyCH4III ACNGT 3 cut(s) 121, 154, 547
HpyCH4IV ACGT 1 cut(s) 181
HpyCH4V TGCA 2 cut(s) 485, 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 74, 194
HpyF3I CTNAG 2 cut(s) 69, 258
HpySE526I ACGT 1 cut(s) 181
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 2 cut(s) 241, 352
HspAI GCGC 1 cut(s) 296
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 1 cut(s) 36
LmnI GCTCC 1 cut(s) 392
LweI GCATC 1 cut(s) 511
MaeI CTAG 1 cut(s) 562
MaeII ACGT 1 cut(s) 181
MaeIII GTNAC 3 cut(s) 121, 541, 547
MalI GATC 1 cut(s) 38
MboI GATC 1 cut(s) 36
MboII GAAGA 1 cut(s) 175
MfeI CAATTG 2 cut(s) 486, 525
MhlI GDGCHC 1 cut(s) 232
MlsI TGGCCA 1 cut(s) 347
MluCI AATT 4 cut(s) 274, 329, 486, 525
MluNI TGGCCA 1 cut(s) 347
MlyI GAGTC 1 cut(s) 89
MnlI CCTC 5 cut(s) 52, 94, 140, 230, 347
Mox20I TGGCCA 1 cut(s) 347
MscI TGGCCA 1 cut(s) 347
MseI TTAA 1 cut(s) 32
Msp20I TGGCCA 1 cut(s) 347
MspI CCGG 4 cut(s) 266, 318, 425, 440
MspR9I CCNGG 1 cut(s) 425
MunI CAATTG 2 cut(s) 486, 525
MwoI GCNNNNNNNGC 3 cut(s) 11, 74, 194
NciI CCSGG 1 cut(s) 425
NdeII GATC 1 cut(s) 36
NlaIII CATG 2 cut(s) 241, 352
NlaIV GGNNCC 1 cut(s) 270
NmuCI GTSAC 1 cut(s) 541
PcsI WCGNNNNNNNCGW 1 cut(s) 371
Pfl23II CGTACG 1 cut(s) 178
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 2 cut(s) 21, 196
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
Ppu21I YACGTR 1 cut(s) 182
PspFI CCCAGC 1 cut(s) 412
PspLI CGTACG 1 cut(s) 178
PspN4I GGNNCC 1 cut(s) 270
PspPI GGNCC 1 cut(s) 268
PsyI GACNNNGTC 1 cut(s) 152
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 2 cut(s) 20, 195
Sau3AI GATC 1 cut(s) 36
Sau96I GGNCC 1 cut(s) 268
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 425
SduI GDGCHC 1 cut(s) 232
SfaNI GCATC 1 cut(s) 511
SinI GGWCC 1 cut(s) 268
Sse9I AATT 4 cut(s) 274, 329, 486, 525
SsiI CCGC 3 cut(s) 20, 195, 251
SspMI CTAG 1 cut(s) 562
StyD4I CCNGG 1 cut(s) 423
TaaI ACNGT 3 cut(s) 121, 154, 547
TaiI ACGT 1 cut(s) 184
TasI AATT 4 cut(s) 274, 329, 486, 525
TauI GCSGC 2 cut(s) 22, 197
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 4 cut(s) 163, 178, 520, 550
TseFI GTSAC 1 cut(s) 541
Tsp45I GTSAC 1 cut(s) 541
TspGWI ACGGA 3 cut(s) 77, 379, 380
TspRI CASTG 4 cut(s) 163, 178, 520, 550
Tth111I GACNNNGTC 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 268
XapI RAATTY 1 cut(s) 329
XcmI CCANNNNNNNNNTGG 1 cut(s) 329
XspI CTAG 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.