Rh6CG018400

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0003188
Physical Location & Seq
Reverse (-)
1842494 .. 1843224
731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG018400.1

Sequence Viewer

Length: 561 bp
ATGGCAAACAACACATTTCCACTTCTGATGATGATGGTCATAATCTCCTTAGCGATAGCATTCACAGAAGCCAAGTGGGCCGAGTCAGTCGAGGCCAAAAAGGAAGCGGCGCCGTCGGAGACAGTAACCAACCTCCAGTTCTTCTTCCACGACACAGTCAGTGGGAAGAACCCTAGTGCCGTACGTGTGGCTCAAGCCTCGGACACCAATAAGTCTCTGACACTTTTCGGGGCTTTACTCATGGTCGATGACCCGCTCACCGAGACTCCCGACCCCAATTCCAAGCTCGTGGGTCGGGCACAAGGTCTATACGGGTCAGCCGGCCAGCAGGAGCTTGGCCTGATTATGGCTTTGAGCTTTGCGTTCACGGATGGTCCTTACAGCGGTAGCTCCATTAGTATATTTGGTAAAAACCCGGCACTGAACCCAGTTCGAGAGTTGCCGGTTGTGGGTGGAACTGGGGTCTTCCGGCTGGCTCGTGGATATGCAATTGCCCATACTCATTGGTTTGATCTTAGTGGTGATGCAATTGTAGGATACAATGTCACTATAATTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

186

Amino Acids

19.85

Weight (kDa)

5.48

Isoelectric Point (pI)

18.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 42 - 184 2.1e-54 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 109
AccBSI CCGCTC 1 cut(s) 256
AciI CCGC 3 cut(s) 107, 254, 384
AcoI YGGCCR 1 cut(s) 322
AcyI GRCGYC 1 cut(s) 110
AfaI GTAC 1 cut(s) 183
AfiI CCNNNNNNNGG 3 cut(s) 346, 383, 449
AflIII ACRYGT 1 cut(s) 184
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 4 cut(s) 286, 334, 357, 390
AluI AGCT 4 cut(s) 286, 334, 357, 390
Alw26I GTCTC 3 cut(s) 113, 219, 257
AoxI GGCC 4 cut(s) 78, 93, 322, 337
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 2 cut(s) 78, 374
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 2 cut(s) 250, 533
AvaII GGWCC 1 cut(s) 374
BaeGI GKGCMC 1 cut(s) 301
BanI GGYRCC 1 cut(s) 109
BauI CACGAG 2 cut(s) 287, 477
BbsI GAAGAC 1 cut(s) 457
BccI CCATC 2 cut(s) 28, 365
BceAI ACGGC 2 cut(s) 97, 164
BciVI GTATCC 1 cut(s) 530
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 3 cut(s) 113, 219, 257
BfaI CTAG 2 cut(s) 174, 559
BfoI RGCGCY 1 cut(s) 113
BfuI GTATCC 1 cut(s) 530
BglI GCCNNNNNGGC 1 cut(s) 77
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 416
Bme18I GGWCC 1 cut(s) 374
BmgT120I GGNCC 2 cut(s) 78, 374
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 1 cut(s) 416
BmrI ACTGGG 2 cut(s) 422, 468
BmsI GCATC 1 cut(s) 514
BmuI ACTGGG 2 cut(s) 422, 468
BpiI GAAGAC 1 cut(s) 457
BpmI CTGGAG 1 cut(s) 119
Bpu10I CCTNAGC 1 cut(s) 49
BpuEI CTTGAG 1 cut(s) 177
BpuMI CCSGG 1 cut(s) 416
BsaAI YACGTR 1 cut(s) 185
BsaHI GRCGYC 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 250, 280
Bsc4I CCNNNNNNNGG 3 cut(s) 346, 383, 449
Bse118I RCCGGY 2 cut(s) 320, 442
Bse1I ACTGG 3 cut(s) 136, 428, 463
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 3 cut(s) 346, 383, 449
BseNI ACTGG 3 cut(s) 136, 428, 463
BseSI GKGCMC 1 cut(s) 301
BshFI GGCC 4 cut(s) 80, 95, 324, 339
BshNI GGYRCC 1 cut(s) 109
BsiSI CCGG 4 cut(s) 321, 416, 443, 469
BsiWI CGTACG 1 cut(s) 181
BslI CCNNNNNNNGG 3 cut(s) 346, 383, 449
BsmAI GTCTC 3 cut(s) 113, 219, 257
BsmI GAATGC 1 cut(s) 59
BsnI GGCC 4 cut(s) 80, 95, 324, 339
Bsp1286I GDGCHC 1 cut(s) 301
Bsp143I GATC 1 cut(s) 511
BspACI CCGC 3 cut(s) 107, 254, 384
BspANI GGCC 4 cut(s) 80, 95, 324, 339
BspLI GGNNCC 1 cut(s) 111
BspT107I GGYRCC 1 cut(s) 109
BsrBI CCGCTC 1 cut(s) 256
BsrFI RCCGGY 2 cut(s) 320, 442
BsrI ACTGG 3 cut(s) 136, 428, 463
BssAI RCCGGY 2 cut(s) 320, 442
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 1 cut(s) 511
BssNI GRCGYC 1 cut(s) 110
BssSI CACGAG 2 cut(s) 287, 477
Bst2BI CACGAG 2 cut(s) 287, 477
Bst4CI ACNGT 2 cut(s) 124, 157
BstACI GRCGYC 1 cut(s) 110
BstBAI YACGTR 1 cut(s) 185
BstC8I GCNNGC 3 cut(s) 322, 326, 474
BstDEI CTNAG 2 cut(s) 49, 515
BstF5I GGATG 1 cut(s) 376
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 1 cut(s) 514
BstMAI GTCTC 3 cut(s) 113, 219, 257
BstMBI GATC 1 cut(s) 511
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 414
BstSLI GKGCMC 1 cut(s) 301
BstV2I GAAGAC 1 cut(s) 457
BstXI CCANNNNNNTGG 1 cut(s) 289
BsuI GTATCC 1 cut(s) 530
BsuRI GGCC 4 cut(s) 80, 95, 324, 339
BtsCI GGATG 1 cut(s) 376
BtsIMutI CAGTG 2 cut(s) 166, 419
Cac8I GCNNGC 3 cut(s) 322, 326, 474
CfoI GCGC 1 cut(s) 112
Cfr10I RCCGGY 2 cut(s) 320, 442
Cfr13I GGNCC 2 cut(s) 78, 374
Csp6I GTAC 1 cut(s) 182
CviAII CATG 1 cut(s) 241
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 2 cut(s) 49, 515
DinI GGCGCC 1 cut(s) 111
DpnI GATC 1 cut(s) 513
DpnII GATC 1 cut(s) 511
EaeI YGGCCR 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 374
EgeI GGCGCC 1 cut(s) 111
EheI GGCGCC 1 cut(s) 111
FaeI CATG 1 cut(s) 244
FaiI YATR 8 cut(s) 41, 242, 310, 347, 401, 486, 498, 551
FatI CATG 1 cut(s) 240
FauI CCCGC 1 cut(s) 261
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 1 cut(s) 383
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 2 cut(s) 174, 559
GlaI GCGC 1 cut(s) 111
GluI GCNGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 119
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 4 cut(s) 80, 95, 324, 339
HapII CCGG 4 cut(s) 321, 416, 443, 469
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 1 cut(s) 110
Hin1II CATG 1 cut(s) 244
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HinfI GANTC 2 cut(s) 83, 265
HpaII CCGG 4 cut(s) 321, 416, 443, 469
HphI GGTGA 2 cut(s) 250, 533
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 4 cut(s) 27, 118, 202, 219
Hpy188III TCNNGA 2 cut(s) 269, 434
Hpy8I GTNNAC 1 cut(s) 366
Hpy99I CGWCG 1 cut(s) 118
HpyCH4III ACNGT 2 cut(s) 124, 157
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 2 cut(s) 488, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 2 cut(s) 49, 515
HpySE526I ACGT 1 cut(s) 184
Hsp92I GRCGYC 1 cut(s) 110
Hsp92II CATG 1 cut(s) 244
HspAI GCGC 1 cut(s) 110
KasI GGCGCC 1 cut(s) 109
KroI GCCGGC 1 cut(s) 320
KroNI GCCGGC 1 cut(s) 322
Kzo9I GATC 1 cut(s) 511
LmnI GCTCC 2 cut(s) 331, 395
LweI GCATC 1 cut(s) 514
MaeI CTAG 2 cut(s) 174, 559
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 2 cut(s) 124, 544
MalI GATC 1 cut(s) 513
MbiI CCGCTC 1 cut(s) 256
MboI GATC 1 cut(s) 511
MboII GAAGA 4 cut(s) 133, 136, 178, 457
MfeI CAATTG 2 cut(s) 489, 528
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 4 cut(s) 277, 489, 528, 552
Mly113I GGCGCC 1 cut(s) 110
MlyI GAGTC 2 cut(s) 92, 259
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 3 cut(s) 85, 143, 208
MroNI GCCGGC 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 384
MspI CCGG 4 cut(s) 321, 416, 443, 469
MspR9I CCNGG 1 cut(s) 416
MunI CAATTG 2 cut(s) 489, 528
Mva1269I GAATGC 1 cut(s) 59
MwoI GCNNNNNNNGC 1 cut(s) 77
NaeI GCCGGC 1 cut(s) 322
NarI GGCGCC 1 cut(s) 110
NciI CCSGG 1 cut(s) 416
NdeII GATC 1 cut(s) 511
NgoMIV GCCGGC 1 cut(s) 320
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 1 cut(s) 111
NmeAIII GCCGAG 1 cut(s) 106
NmuCI GTSAC 1 cut(s) 544
PctI GAATGC 1 cut(s) 59
PdiI GCCGGC 1 cut(s) 322
Pfl23II CGTACG 1 cut(s) 181
PflFI GACNNNGTC 1 cut(s) 155
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 2 cut(s) 91, 259
PluTI GGCGCC 1 cut(s) 113
PpsI GAGTC 2 cut(s) 91, 259
Ppu21I YACGTR 1 cut(s) 185
PspLI CGTACG 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 2 cut(s) 78, 374
PsyI GACNNNGTC 1 cut(s) 155
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 1 cut(s) 511
Sau96I GGNCC 2 cut(s) 78, 374
SchI GAGTC 2 cut(s) 92, 259
ScrFI CCNGG 1 cut(s) 416
SduI GDGCHC 1 cut(s) 301
SetI ASST 7 cut(s) 135, 187, 288, 307, 336, 359, 392
SfaNI GCATC 1 cut(s) 514
SfoI GGCGCC 1 cut(s) 111
SinI GGWCC 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 192
SmoI CTYRAG 1 cut(s) 192
Sse9I AATT 4 cut(s) 277, 489, 528, 552
SsiI CCGC 3 cut(s) 107, 254, 384
SspDI GGCGCC 1 cut(s) 109
SspMI CTAG 2 cut(s) 174, 559
StyD4I CCNGG 1 cut(s) 414
TaaI ACNGT 2 cut(s) 124, 157
TaiI ACGT 1 cut(s) 187
TaqI TCGA 3 cut(s) 90, 246, 433
TasI AATT 4 cut(s) 277, 489, 528, 552
TauI GCSGC 1 cut(s) 110
TscAI CASTG 2 cut(s) 166, 426
TseFI GTSAC 1 cut(s) 544
Tsp45I GTSAC 1 cut(s) 544
TspGWI ACGGA 1 cut(s) 383
TspRI CASTG 2 cut(s) 166, 426
Tth111I GACNNNGTC 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 374
XcmI CCANNNNNNNNNTGG 1 cut(s) 332
XspI CTAG 2 cut(s) 174, 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.