MD05G1117300.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003188
Physical Location & Seq
Forward (+)
23826909 .. 23827490
582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1117300.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGCAAACGCAGCCGTGACTGTGATGCTCGTGCTCATCATCGCCGTCGTCGCTGCAACCACCGAAGGAGCTCATAGCAGATCCACACAACCCAAGTGGGCCAAGAAGCTCAAGACAAGGAAGAAAATGACGTCGTCAGAGACAGTGACCAACCTCCAATTCTACTTCCACGACACAGTCAGTGGGGAGAACCCAAGTGCCGTCCGTATAGCCCAAGCCACAGACACCAACAAGTCCCTCACCCTTTTCGGAGCTCTGCTCATGGTAGATGACCCGCTAACCGAAACGCCTGACCCGAATTCCAAGCTCGTGGGCCGGGCGCAGGGGATGTACGGGTCATCCGGGCAGCAAGAGTTTGGGCTTACTATGGCCATGTGCTTCGCGTTCTGTGATGGCATTTACGATGGGAGCTCGATTAGTATTTTCGGTAAGAACCCGGCTCTGAATCTGGTTCGGGAGATGCCGGTTGTGGCCGGAACTGGCATTTTCCAGTTCGCCCGGGGTTATGCAATTGCCCGGACGCATTGGTTTAATATTATGGGCGATGCAATTGTTGAATACAATGTCACTGTTATTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

194

Amino Acids

20.89

Weight (kDa)

7.79

Isoelectric Point (pI)

27.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 49 - 191 3.8e-52 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 134
AccII CGCG 1 cut(s) 383
AciI CCGC 1 cut(s) 275
AclWI GGATC 1 cut(s) 75
AcoI YGGCCR 2 cut(s) 369, 471
AcsI RAATTY 1 cut(s) 298
AcyI GRCGYC 1 cut(s) 131
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 1 cut(s) 322
AgsI TTSAA 1 cut(s) 557
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AluBI AGCT 5 cut(s) 71, 109, 254, 307, 411
AluI AGCT 5 cut(s) 71, 109, 254, 307, 411
Alw21I GWGCWC 4 cut(s) 36, 73, 256, 413
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 1 cut(s) 75
Ama87I CYCGRG 1 cut(s) 498
AoxI GGCC 4 cut(s) 99, 313, 369, 471
ApeKI GCWGC 3 cut(s) 11, 53, 346
ApoI RAATTY 1 cut(s) 298
AspLEI GCGC 1 cut(s) 322
AspS9I GGNCC 2 cut(s) 99, 313
AsuC2I CCSGG 6 cut(s) 317, 343, 437, 499, 500, 517
AsuHPI GGTGA 1 cut(s) 232
AvaI CYCGRG 1 cut(s) 498
BalI TGGCCA 1 cut(s) 371
BanII GRGCYC 3 cut(s) 73, 256, 413
BauI CACGAG 2 cut(s) 29, 308
Bbv12I GWGCWC 4 cut(s) 36, 73, 256, 413
BbvI GCAGC 3 cut(s) 23, 40, 358
BccI CCATC 2 cut(s) 386, 398
BceAI ACGGC 2 cut(s) 29, 185
BcnI CCSGG 6 cut(s) 317, 343, 437, 499, 500, 517
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 1 cut(s) 580
BisI GCNGC 3 cut(s) 12, 54, 347
BlsI GCNGC 3 cut(s) 13, 55, 348
Bme1390I CCNGG 6 cut(s) 317, 343, 437, 499, 500, 517
BmeT110I CYCGRG 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 99, 313
BmrFI CCNGG 6 cut(s) 317, 343, 437, 499, 500, 517
BmsI GCATC 3 cut(s) 15, 450, 535
BplI GAGNNNNNCTC 2 cut(s) 243, 275
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 6 cut(s) 317, 343, 437, 499, 500, 517
BsaHI GRCGYC 1 cut(s) 131
BsaJI CCNNGG 2 cut(s) 498, 499
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse118I RCCGGY 1 cut(s) 463
Bse1I ACTGG 2 cut(s) 484, 490
BseDI CCNNGG 2 cut(s) 498, 499
BseGI GGATG 2 cut(s) 333, 338
BseLI CCNNNNNNNGG 1 cut(s) 322
BseNI ACTGG 2 cut(s) 484, 490
BseXI GCAGC 3 cut(s) 23, 40, 358
Bsh1236I CGCG 1 cut(s) 383
BshFI GGCC 4 cut(s) 101, 315, 371, 473
BsiHKAI GWGCWC 4 cut(s) 36, 73, 256, 413
BsiHKCI CYCGRG 1 cut(s) 498
BsiSI CCGG 7 cut(s) 316, 342, 437, 464, 474, 499, 517
BslFI GGGAC 1 cut(s) 220
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 220
BsnI GGCC 4 cut(s) 101, 315, 371, 473
BsoBI CYCGRG 1 cut(s) 498
Bsp1286I GDGCHC 4 cut(s) 36, 73, 256, 413
Bsp143I GATC 1 cut(s) 80
BspACI CCGC 1 cut(s) 275
BspANI GGCC 4 cut(s) 101, 315, 371, 473
BspFNI CGCG 1 cut(s) 383
BspPI GGATC 1 cut(s) 75
BsrFI RCCGGY 1 cut(s) 463
BsrI ACTGG 2 cut(s) 484, 490
BssAI RCCGGY 1 cut(s) 463
BssECI CCNNGG 2 cut(s) 498, 499
BssMI GATC 1 cut(s) 80
BssNI GRCGYC 1 cut(s) 131
BssSI CACGAG 2 cut(s) 29, 308
Bst2BI CACGAG 2 cut(s) 29, 308
Bst4CI ACNGT 4 cut(s) 22, 145, 178, 571
BstACI GRCGYC 1 cut(s) 131
BstF5I GGATG 2 cut(s) 333, 338
BstFNI CGCG 1 cut(s) 383
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 1 cut(s) 83
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 1 cut(s) 80
BstMWI GCNNNNNNNGC 2 cut(s) 11, 50
BstSCI CCNGG 6 cut(s) 315, 341, 435, 497, 498, 515
BstUI CGCG 1 cut(s) 383
BstV1I GCAGC 3 cut(s) 23, 40, 358
BstX2I RGATCY 1 cut(s) 80
BstXI CCANNNNNNTGG 1 cut(s) 310
BstYI RGATCY 1 cut(s) 80
BsuRI GGCC 4 cut(s) 101, 315, 371, 473
BtgZI GCGATG 2 cut(s) 25, 558
BtsCI GGATG 2 cut(s) 333, 338
BtsIMutI CAGTG 3 cut(s) 150, 187, 567
CfoI GCGC 1 cut(s) 322
Cfr10I RCCGGY 1 cut(s) 463
Cfr13I GGNCC 2 cut(s) 99, 313
Cfr9I CCCGGG 1 cut(s) 498
CseI GACGC 1 cut(s) 529
Csp6I GTAC 1 cut(s) 331
CspCI CAANNNNNGTGG 2 cut(s) 77, 112
CviAII CATG 2 cut(s) 262, 373
CviQI GTAC 1 cut(s) 331
DpnI GATC 1 cut(s) 82
DpnII GATC 1 cut(s) 80
EaeI YGGCCR 2 cut(s) 369, 471
Ecl136II GAGCTC 3 cut(s) 71, 254, 411
Eco24I GRGCYC 3 cut(s) 73, 256, 413
Eco53kI GAGCTC 3 cut(s) 71, 254, 411
Eco88I CYCGRG 1 cut(s) 498
EcoICRI GAGCTC 3 cut(s) 71, 254, 411
EcoRI GAATTC 1 cut(s) 298
EcoT38I GRGCYC 3 cut(s) 73, 256, 413
FaeI CATG 2 cut(s) 265, 376
FaiI YATR 7 cut(s) 75, 209, 263, 368, 374, 507, 539
FaqI GGGAC 1 cut(s) 220
FatI CATG 2 cut(s) 261, 372
FauI CCCGC 1 cut(s) 282
Fnu4HI GCNGC 3 cut(s) 12, 54, 347
FokI GGATG 2 cut(s) 325, 340
FriOI GRGCYC 3 cut(s) 73, 256, 413
Fsp4HI GCNGC 3 cut(s) 12, 54, 347
FspBI CTAG 1 cut(s) 580
GlaI GCGC 1 cut(s) 321
GluI GCNGC 3 cut(s) 12, 54, 347
HaeIII GGCC 4 cut(s) 101, 315, 371, 473
HapII CCGG 7 cut(s) 316, 342, 437, 464, 474, 499, 517
HgaI GACGC 1 cut(s) 529
HhaI GCGC 1 cut(s) 322
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 2 cut(s) 265, 376
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HinfI GANTC 1 cut(s) 445
HpaII CCGG 7 cut(s) 316, 342, 437, 464, 474, 499, 517
HphI GGTGA 1 cut(s) 232
Hpy188I TCNGA 3 cut(s) 139, 251, 444
Hpy188III TCNNGA 2 cut(s) 112, 455
Hpy99I CGWCG 3 cut(s) 50, 53, 136
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 4 cut(s) 22, 145, 178, 571
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 3 cut(s) 56, 509, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 50
HpySE526I ACGT 1 cut(s) 131
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 2 cut(s) 265, 376
HspAI GCGC 1 cut(s) 320
Kzo9I GATC 1 cut(s) 80
LmnI GCTCC 3 cut(s) 68, 251, 408
Lsp1109I GCAGC 3 cut(s) 23, 40, 358
LweI GCATC 3 cut(s) 15, 450, 535
MaeI CTAG 1 cut(s) 580
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 3 cut(s) 16, 145, 565
MalI GATC 1 cut(s) 82
MboI GATC 1 cut(s) 80
MboII GAAGA 1 cut(s) 133
MfeI CAATTG 2 cut(s) 510, 549
MflI RGATCY 1 cut(s) 80
MhlI GDGCHC 4 cut(s) 36, 73, 256, 413
MlsI TGGCCA 1 cut(s) 371
MluCI AATT 4 cut(s) 158, 298, 510, 549
MluNI TGGCCA 1 cut(s) 371
MnlI CCTC 2 cut(s) 164, 248
Mox20I TGGCCA 1 cut(s) 371
MscI TGGCCA 1 cut(s) 371
MseI TTAA 1 cut(s) 531
Msp20I TGGCCA 1 cut(s) 371
MspI CCGG 7 cut(s) 316, 342, 437, 464, 474, 499, 517
MspR9I CCNGG 6 cut(s) 317, 343, 437, 499, 500, 517
MunI CAATTG 2 cut(s) 510, 549
MvnI CGCG 1 cut(s) 383
MwoI GCNNNNNNNGC 2 cut(s) 11, 50
NciI CCSGG 6 cut(s) 317, 343, 437, 499, 500, 517
NdeII GATC 1 cut(s) 80
NlaIII CATG 2 cut(s) 265, 376
NmuCI GTSAC 3 cut(s) 16, 145, 565
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PfeI GAWTC 1 cut(s) 445
PflFI GACNNNGTC 2 cut(s) 133, 176
PkrI GCNGC 3 cut(s) 13, 55, 348
Psp124BI GAGCTC 3 cut(s) 73, 256, 413
PspPI GGNCC 2 cut(s) 99, 313
PsuI RGATCY 1 cut(s) 80
PsyI GACNNNGTC 2 cut(s) 133, 176
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
SacI GAGCTC 3 cut(s) 73, 256, 413
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 3 cut(s) 12, 54, 347
Sau3AI GATC 1 cut(s) 80
Sau96I GGNCC 2 cut(s) 99, 313
ScrFI CCNGG 6 cut(s) 317, 343, 437, 499, 500, 517
SduI GDGCHC 4 cut(s) 36, 73, 256, 413
SetI ASST 7 cut(s) 73, 111, 134, 156, 256, 309, 413
SfaNI GCATC 3 cut(s) 15, 450, 535
SmaI CCCGGG 1 cut(s) 500
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 4 cut(s) 158, 298, 510, 549
SsiI CCGC 1 cut(s) 275
SspI AATATT 1 cut(s) 535
SspMI CTAG 1 cut(s) 580
SstI GAGCTC 3 cut(s) 73, 256, 413
StyD4I CCNGG 6 cut(s) 315, 341, 435, 497, 498, 515
TaaI ACNGT 4 cut(s) 22, 145, 178, 571
TaiI ACGT 1 cut(s) 134
TaqI TCGA 1 cut(s) 413
TasI AATT 4 cut(s) 158, 298, 510, 549
TfiI GAWTC 1 cut(s) 445
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 3 cut(s) 150, 187, 574
TseFI GTSAC 3 cut(s) 16, 145, 565
TseI GCWGC 3 cut(s) 11, 53, 346
Tsp45I GTSAC 3 cut(s) 16, 145, 565
TspGWI ACGGA 1 cut(s) 194
TspMI CCCGGG 1 cut(s) 498
TspRI CASTG 3 cut(s) 150, 187, 574
Tth111I GACNNNGTC 2 cut(s) 133, 176
XapI RAATTY 1 cut(s) 298
XmaI CCCGGG 1 cut(s) 498
XspI CTAG 1 cut(s) 580
ZraI GACGTC 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.