Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003439

Physical Location & Seq

Genomic Coordinates Forward (+)

14465516 .. 14468547

Gene Structure 3032 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G34260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1062 bp

ATGGAGATCGATTTGGGAGCTAACGCGTTTGGTATCGACTTCCATCCATCGACGAATCTAGTAGCTGCTGGTCTCATCGATGGCCACTTGCATCTATACCGATACGACTCAGATTCTTCACTTGTCAGGGAACGTAAAGTTCGTGCCCATAAGGAGTCTTGTAGAGCTGTTCGGTTCATTGATGATGGCCAAAGAATCGTCACTGCTTCAGCTGATTGTTCAATTCTAGCAACTGATGTAGAGACTGGTGCGCAGGTTGCACATCTTGAGAATGCTCACGAGGATGCTGTTAATACTTTGATTAATGTTACTGAGACAACTATCGCTTCAGGAGATGATAAAGGCTGCGTTAAGATTTGGGATACAAGACAGCGCTCTTGCTCTCATGAGTTTAATGCACACGAGGATTACATTTCTGGCATGACCTTTGCATCTGATTCAATGAAGCTAGTGGTAACAAGTGGAGACGGGACTTTGTCTGTCTGTAATCTTAGAACGAGTAAGGTCCAATCTCAGTCCGAATTTTCTGAAGATGAACTACTTTCTGTTGTTATAATGAAGAATGGCCGTAAAGTTATCTGTGGAACTCAGAATGGTACTCTCTTGTTGTATTCATGGGGATTTTTCAAGGATTGCAGCGATCGGTTTGTTGATCTCGCTCCAAATTCCGTTGATGCTCTACTGAAGCTCGATGAGGACAGACTTATCACTGGATGTGATAATGGAATAATTAGCCTTGTTGGAATACTGCCCAACAGAATCATACAGCCGATTGGGTCACACGACTACCCTATCGAAGATCTCGCTCTCTCGCATGATAAGAAATTTCTCGGTAGCACAGCTCATGACAGTATGCTGAAGTTGTGGAACCTAGAAGAGATTCTAGAAGGTTCTAATGTCAACTCTGGGAATGCATCTGGAGCTGCAGAAGACAGTGACAGCGACAATGATGGGATGGACCTTGACAATGATCCTTCCAAGTCTTCCAAAGGCAGCAAGAGGAAAACAAAAAGTAAAGCGAATACGCTGAACGCTACAAATAATTTCTTTGCAGACTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000741 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0006997 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009553 GO:0009559 GO:0009561 GO:0009790 GO:0009791 GO:0009793 GO:0009799 GO:0009855 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010197 GO:0010467 GO:0016043 GO:0016070 GO:0016072 GO:0022414 GO:0022613 GO:0030154 GO:0032501 GO:0032502 GO:0034470 GO:0034641 GO:0034660 GO:0040007 GO:0042254 GO:0042273 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048229 GO:0048284 GO:0048316 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0061458 GO:0071704 GO:0071840 GO:0080186 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANAPC4_WD40 WD40

Protein Analysis

353

Amino Acids

38.43

Weight (kDa)

4.87

Isoelectric Point (pI)

23.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WDR55	PF24796	2 - 293	9.3e-101	WDR55
WD40_WDHD1_1st	PF24817	5 - 136	5.3e-08	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	8 - 92	7.8e-08	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	12 - 161	4.9e-20	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	12 - 92	2.2e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	12 - 183	1.3e-18	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	12 - 205	2e-22	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	13 - 196	1.6e-17	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st	PF25171	16 - 104	8.8e-06	WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz	PF24807	17 - 170	3.1e-15	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	18 - 169	8.1e-11	WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	35 - 169	3.4e-12	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	50 - 293	2e-28	THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd	PF23393	64 - 169	3.8e-07	WDR90/POC16, second beta-propeller
EIF3I	PF24805	83 - 192	2.8e-06	EIF3I
Beta-prop_WDR3_1st	PF25173	91 - 205	1.1e-12	WDR3 first beta-propeller domain
Beta-prop_Vps41	PF23411	95 - 249	1.9e-07	Vps42 beta-propeller
Beta-prop_WDR3_2nd	PF25172	101 - 211	2e-08	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	106 - 206	8.9e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	111 - 290	6.3e-06	WDR36/Utp21 first beta-propeller
Beta-prop_EML	PF23409	112 - 205	9.9e-07	Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st	PF24817	133 - 294	7.9e-06	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	163 - 294	3.7e-07	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	163 - 293	5.7e-07	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	183 - 292	3.8e-06	WDR36/Utp21 second beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003439)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34260 AT2G34260
fragaria_vesca	FvH4_6g22900 FvH4_6g22900 FvH4_6g22900 FvH4_6g22900 FvH4_6g22900
malus_domestica	MD12G1063000.v1.1 MD14G1063600.v1.1
prunus_persica	Prupe.7G070600_v2.0.a1
pyrus_communis	pycom12g05550 pycom14g05140
rosa_chinensis	RchiOBHm_Chr3g0480691 RchiOBHm_Chr4g0410631 RchiOBHm_Chr5g0032061
rosa_laevigata	RLG00000023461
rosa_multiflora	Rmu_sc0001135.1_g000010 Rmu_sc0006144.1_g000003 Rmu_sc0010172.1_g000004
rosa_roxburghii	Rroxscaffold_5G00355420 Rroxscaffold_6G00401160
rosa_rugosa	Rorug03G0184600 Rorug03G0184700.1
rosa_samantha	Rh3AG237000 Rh3BG270100 Rh3DG262400 Rh4AG161900 Rh4AG162000 Rh4BG160900 Rh4BG161000 Rh4CG174900 Rh4CG175000 Rh4DG157800 Rh7AG489700
rosa_wichuraiana	Rw3G021220 Rw4G013510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	554
Acc16I	TGCGCA	1 cut(s)	252
Acc36I	ACCTGC	1 cut(s)	244
AccII	CGCG	1 cut(s)	26
AclWI	GGATC	1 cut(s)	965
AcoI	YGGCCR	3 cut(s)	82, 187, 565
AcsI	RAATTY	3 cut(s)	521, 664, 824
AcuI	CTGAAG	5 cut(s)	192, 312, 549, 704, 878
AfaI	GTAC	1 cut(s)	598
AfeI	AGCGCT	1 cut(s)	374
AflIII	ACRYGT	1 cut(s)	24
AgsI	TTSAA	3 cut(s)	222, 441, 628
AluBI	AGCT	8 cut(s)	20, 65, 167, 212, 448, 688, 842, 923
AluI	AGCT	8 cut(s)	20, 65, 167, 212, 448, 688, 842, 923
Alw26I	GTCTC	4 cut(s)	77, 236, 308, 459
AlwI	GGATC	1 cut(s)	965
Aor51HI	AGCGCT	1 cut(s)	374
AoxI	GGCC	3 cut(s)	82, 187, 565
ApeKI	GCWGC	5 cut(s)	65, 345, 636, 923, 993
ApoI	RAATTY	3 cut(s)	521, 664, 824
AseI	ATTAAT	1 cut(s)	303
Asp700I	GAANNNNTTC	1 cut(s)	879
AspLEI	GCGC	2 cut(s)	253, 375
AspS9I	GGNCC	2 cut(s)	505, 958
AvaII	GGWCC	2 cut(s)	505, 958
BaeGI	GKGCMC	1 cut(s)	148
BalI	TGGCCA	2 cut(s)	84, 189
BarI	GAAGNNNNNNTAC	2 cut(s)	522, 554
BauI	CACGAG	2 cut(s)	278, 401
BbsI	GAAGAC	2 cut(s)	936, 975
BbvI	GCAGC	5 cut(s)	52, 332, 648, 910, 1005
BccI	CCATC	6 cut(s)	51, 55, 74, 179, 944, 949
BceAI	ACGGC	1 cut(s)	552
BciVI	GTATCC	1 cut(s)	355
BcoDI	GTCTC	4 cut(s)	77, 236, 308, 459
BfaI	CTAG	5 cut(s)	59, 227, 449, 872, 884
BfmI	CTRYAG	1 cut(s)	924
BfoI	RGCGCY	1 cut(s)	376
BfuAI	ACCTGC	1 cut(s)	244
BfuI	GTATCC	1 cut(s)	355
BglII	AGATCT	1 cut(s)	799
BisI	GCNGC	5 cut(s)	66, 346, 637, 924, 994
BlsI	GCNGC	5 cut(s)	67, 347, 638, 925, 995
Bme18I	GGWCC	2 cut(s)	505, 958
BmgT120I	GGNCC	2 cut(s)	505, 958
BmiI	GGNNCC	1 cut(s)	869
BmsI	GCATC	5 cut(s)	100, 274, 440, 664, 923
BpiI	GAAGAC	2 cut(s)	936, 975
BpmI	CTGGAG	1 cut(s)	939
BpuEI	CTTGAG	1 cut(s)	287
Bsa29I	ATCGAT	2 cut(s)	9, 78
BsaI	GGTCTC	1 cut(s)	77
Bse1I	ACTGG	2 cut(s)	250, 715
BseCI	ATCGAT	2 cut(s)	9, 78
BseGI	GGATG	4 cut(s)	43, 289, 719, 960
BseMII	CTCAG	4 cut(s)	123, 303, 527, 602
BseNI	ACTGG	2 cut(s)	250, 715
BseSI	GKGCMC	1 cut(s)	148
BseXI	GCAGC	5 cut(s)	52, 332, 648, 910, 1005
Bsh1236I	CGCG	1 cut(s)	26
Bsh1285I	CGRYCG	1 cut(s)	643
BshFI	GGCC	3 cut(s)	84, 189, 567
BshVI	ATCGAT	2 cut(s)	9, 78
BsiEI	CGRYCG	1 cut(s)	643
BslFI	GGGAC	1 cut(s)	484
BsmAI	GTCTC	4 cut(s)	77, 236, 308, 459
BsmBI	CGTCTC	1 cut(s)	459
BsmFI	GGGAC	1 cut(s)	484
BsmI	GAATGC	2 cut(s)	277, 916
BsnI	GGCC	3 cut(s)	84, 189, 567
Bso31I	GGTCTC	1 cut(s)	77
Bsp1286I	GDGCHC	1 cut(s)	148
Bsp143I	GATC	5 cut(s)	6, 640, 652, 799, 970
BspANI	GGCC	3 cut(s)	84, 189, 567
BspCNI	CTCAG	4 cut(s)	122, 304, 526, 601
BspDI	ATCGAT	2 cut(s)	9, 78
BspFNI	CGCG	1 cut(s)	26
BspHI	TCATGA	2 cut(s)	385, 844
BspLI	GGNNCC	1 cut(s)	869
BspMAI	CTGCAG	1 cut(s)	928
BspMI	ACCTGC	1 cut(s)	244
BspPI	GGATC	1 cut(s)	965
BspTNI	GGTCTC	1 cut(s)	77
BsrI	ACTGG	2 cut(s)	250, 715
BssMI	GATC	5 cut(s)	6, 640, 652, 799, 970
BssSI	CACGAG	2 cut(s)	278, 401
Bst2BI	CACGAG	2 cut(s)	278, 401
Bst4CI	ACNGT	2 cut(s)	851, 935
Bst6I	CTCTTC	1 cut(s)	870
BstDEI	CTNAG	5 cut(s)	109, 312, 491, 513, 588
BstF5I	GGATG	4 cut(s)	43, 289, 719, 960
BstFNI	CGCG	1 cut(s)	26
BstH2I	RGCGCY	1 cut(s)	376
BstHHI	GCGC	2 cut(s)	253, 375
BstKTI	GATC	5 cut(s)	9, 643, 655, 802, 973
BstMAI	GTCTC	4 cut(s)	77, 236, 308, 459
BstMBI	GATC	5 cut(s)	6, 640, 652, 799, 970
BstMCI	CGRYCG	1 cut(s)	643
BstMWI	GCNNNNNNNGC	2 cut(s)	257, 920
BstSFI	CTRYAG	1 cut(s)	924
BstSLI	GKGCMC	1 cut(s)	148
BstUI	CGCG	1 cut(s)	26
BstV1I	GCAGC	5 cut(s)	52, 332, 648, 910, 1005
BstV2I	GAAGAC	2 cut(s)	936, 975
BstX2I	RGATCY	1 cut(s)	799
BstYI	RGATCY	1 cut(s)	799
Bsu15I	ATCGAT	2 cut(s)	9, 78
BsuI	GTATCC	1 cut(s)	355
BsuRI	GGCC	3 cut(s)	84, 189, 567
BsuTUI	ATCGAT	2 cut(s)	9, 78
BtsCI	GGATG	4 cut(s)	43, 289, 719, 960
BtsI	GCAGTG	1 cut(s)	201
BtsIMutI	CAGTG	3 cut(s)	201, 708, 940
BveI	ACCTGC	1 cut(s)	244
CciI	TCATGA	2 cut(s)	385, 844
CfoI	GCGC	2 cut(s)	253, 375
Cfr13I	GGNCC	2 cut(s)	505, 958
ClaI	ATCGAT	2 cut(s)	9, 78
Csp6I	GTAC	1 cut(s)	597
CviAII	CATG	5 cut(s)	386, 421, 615, 815, 845
CviQI	GTAC	1 cut(s)	597
DdeI	CTNAG	5 cut(s)	109, 312, 491, 513, 588
DpnI	GATC	5 cut(s)	8, 642, 654, 801, 972
DpnII	GATC	5 cut(s)	6, 640, 652, 799, 970
EaeI	YGGCCR	3 cut(s)	82, 187, 565
Eam1104I	CTCTTC	1 cut(s)	870
EarI	CTCTTC	1 cut(s)	870
Eco31I	GGTCTC	1 cut(s)	77
Eco47I	GGWCC	2 cut(s)	505, 958
Eco47III	AGCGCT	1 cut(s)	374
Eco57I	CTGAAG	5 cut(s)	192, 312, 549, 704, 878
EcoT22I	ATGCAT	1 cut(s)	916
Esp3I	CGTCTC	1 cut(s)	459
FaeI	CATG	5 cut(s)	389, 424, 618, 818, 848
FaqI	GGGAC	1 cut(s)	484
FatI	CATG	5 cut(s)	385, 420, 614, 814, 844
Fnu4HI	GCNGC	5 cut(s)	66, 346, 637, 924, 994
FokI	GGATG	4 cut(s)	30, 296, 726, 967
Fsp4HI	GCNGC	5 cut(s)	66, 346, 637, 924, 994
FspBI	CTAG	5 cut(s)	59, 227, 449, 872, 884
FspI	TGCGCA	1 cut(s)	252
GlaI	GCGC	2 cut(s)	252, 374
GluI	GCNGC	5 cut(s)	66, 346, 637, 924, 994
GsuI	CTGGAG	1 cut(s)	939
HaeII	RGCGCY	1 cut(s)	376
HaeIII	GGCC	3 cut(s)	84, 189, 567
HhaI	GCGC	2 cut(s)	253, 375
Hin1II	CATG	5 cut(s)	389, 424, 618, 818, 848
Hin6I	GCGC	2 cut(s)	251, 373
HinP1I	GCGC	2 cut(s)	251, 373
HincII	GTYRAC	1 cut(s)	901
HindII	GTYRAC	1 cut(s)	901
HinfI	GANTC	8 cut(s)	55, 107, 113, 155, 195, 437, 759, 880
Hpy166II	GTNNAC	1 cut(s)	901
Hpy188I	TCNGA	5 cut(s)	112, 436, 520, 529, 591
Hpy188III	TCNNGA	7 cut(s)	266, 278, 330, 386, 845, 884, 918
Hpy8I	GTNNAC	1 cut(s)	901
Hpy99I	CGWCG	1 cut(s)	55
HpyAV	CCTTC	2 cut(s)	881, 984
HpyCH4III	ACNGT	2 cut(s)	851, 935
HpyCH4IV	ACGT	1 cut(s)	133
HpyCH4V	TGCA	8 cut(s)	91, 260, 398, 431, 636, 914, 926, 1052
HpyF10VI	GCNNNNNNNGC	2 cut(s)	257, 920
HpyF3I	CTNAG	5 cut(s)	109, 312, 491, 513, 588
HpySE526I	ACGT	1 cut(s)	133
Hsp92II	CATG	5 cut(s)	389, 424, 618, 818, 848
HspAI	GCGC	2 cut(s)	251, 373
Kzo9I	GATC	5 cut(s)	6, 640, 652, 799, 970
LmnI	GCTCC	3 cut(s)	17, 664, 920
LpnPI	CCDG	9 cut(s)	54, 112, 231, 239, 315, 402, 696, 891, 903
Lsp1109I	GCAGC	5 cut(s)	52, 332, 648, 910, 1005
LweI	GCATC	5 cut(s)	100, 274, 440, 664, 923
MaeI	CTAG	5 cut(s)	59, 227, 449, 872, 884
MaeII	ACGT	1 cut(s)	133
MaeIII	GTNAC	5 cut(s)	199, 307, 454, 777, 935
MalI	GATC	5 cut(s)	8, 642, 654, 801, 972
MboI	GATC	5 cut(s)	6, 640, 652, 799, 970
MboII	GAAGA	7 cut(s)	108, 542, 571, 809, 887, 941, 975
MflI	RGATCY	1 cut(s)	799
MhlI	GDGCHC	1 cut(s)	148
MlsI	TGGCCA	2 cut(s)	84, 189
MluCI	AATT	6 cut(s)	222, 521, 664, 729, 824, 1042
MluI	ACGCGT	1 cut(s)	24
MluNI	TGGCCA	2 cut(s)	84, 189
MlyI	GAGTC	2 cut(s)	101, 164
MmeI	TCCRAC	1 cut(s)	721
MnlI	CCTC	4 cut(s)	274, 397, 688, 993
Mox20I	TGGCCA	2 cut(s)	84, 189
Mph1103I	ATGCAT	1 cut(s)	916
MroXI	GAANNNNTTC	1 cut(s)	879
MscI	TGGCCA	2 cut(s)	84, 189
MseI	TTAA	4 cut(s)	291, 303, 351, 393
MslI	CAYNNNNRTG	1 cut(s)	282
Msp20I	TGGCCA	2 cut(s)	84, 189
MspA1I	CMGCKG	1 cut(s)	212
Mva1269I	GAATGC	2 cut(s)	277, 916
MvnI	CGCG	1 cut(s)	26
MwoI	GCNNNNNNNGC	2 cut(s)	257, 920
NdeII	GATC	5 cut(s)	6, 640, 652, 799, 970
NlaIII	CATG	5 cut(s)	389, 424, 618, 818, 848
NlaIV	GGNNCC	1 cut(s)	869
NmuCI	GTSAC	3 cut(s)	199, 777, 935
NsbI	TGCGCA	1 cut(s)	252
NsiI	ATGCAT	1 cut(s)	916
PagI	TCATGA	2 cut(s)	385, 844
PcsI	WCGNNNNNNNCGW	1 cut(s)	139
PctI	GAATGC	2 cut(s)	277, 916
PdmI	GAANNNNTTC	1 cut(s)	879
PfeI	GAWTC	6 cut(s)	55, 113, 195, 437, 759, 880
PflFI	GACNNNGTC	1 cut(s)	475
PkrI	GCNGC	5 cut(s)	67, 347, 638, 925, 995
Ple19I	CGATCG	1 cut(s)	643
PleI	GAGTC	2 cut(s)	101, 163
PpsI	GAGTC	2 cut(s)	101, 163
PshBI	ATTAAT	1 cut(s)	303
PsiI	TTATAA	1 cut(s)	554
PspN4I	GGNNCC	1 cut(s)	869
PspPI	GGNCC	2 cut(s)	505, 958
PstI	CTGCAG	1 cut(s)	928
PsuI	RGATCY	1 cut(s)	799
PsyI	GACNNNGTC	1 cut(s)	475
PvuI	CGATCG	1 cut(s)	643
PvuII	CAGCTG	1 cut(s)	212
RsaI	GTAC	1 cut(s)	598
RsaNI	GTAC	1 cut(s)	597
RseI	CAYNNNNRTG	1 cut(s)	282
SaqAI	TTAA	4 cut(s)	291, 303, 351, 393
SatI	GCNGC	5 cut(s)	66, 346, 637, 924, 994
Sau3AI	GATC	5 cut(s)	6, 640, 652, 799, 970
Sau96I	GGNCC	2 cut(s)	505, 958
SchI	GAGTC	2 cut(s)	101, 164
SduI	GDGCHC	1 cut(s)	148
SfaNI	GCATC	5 cut(s)	100, 274, 440, 664, 923
SfcI	CTRYAG	1 cut(s)	924
SinI	GGWCC	2 cut(s)	505, 958
SmiMI	CAYNNNNRTG	1 cut(s)	282
SmlI	CTYRAG	1 cut(s)	266
SmoI	CTYRAG	1 cut(s)	266
Sse9I	AATT	6 cut(s)	222, 521, 664, 729, 824, 1042
SspMI	CTAG	5 cut(s)	59, 227, 449, 872, 884
TaaI	ACNGT	2 cut(s)	851, 935
TaiI	ACGT	1 cut(s)	136
TaqI	TCGA	6 cut(s)	9, 36, 50, 78, 690, 795
TasI	AATT	6 cut(s)	222, 521, 664, 729, 824, 1042
TfiI	GAWTC	6 cut(s)	55, 113, 195, 437, 759, 880
Tru1I	TTAA	4 cut(s)	291, 303, 351, 393
Tru9I	TTAA	4 cut(s)	291, 303, 351, 393
TscAI	CASTG	3 cut(s)	208, 715, 940
TseFI	GTSAC	3 cut(s)	199, 777, 935
TseI	GCWGC	5 cut(s)	65, 345, 636, 923, 993
Tsp45I	GTSAC	3 cut(s)	199, 777, 935
TspDTI	ATGAA	5 cut(s)	166, 458, 549, 572, 603
TspGWI	ACGGA	1 cut(s)	658
TspRI	CASTG	3 cut(s)	208, 715, 940
Tth111I	GACNNNGTC	1 cut(s)	475
VpaK11BI	GGWCC	2 cut(s)	505, 958
VspI	ATTAAT	1 cut(s)	303
XapI	RAATTY	3 cut(s)	521, 664, 824
XbaI	TCTAGA	1 cut(s)	883
XmnI	GAANNNNTTC	1 cut(s)	879
XspI	CTAG	5 cut(s)	59, 227, 449, 872, 884
Zsp2I	ATGCAT	1 cut(s)	916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.