Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005346

Physical Location & Seq

Genomic Coordinates Forward (+)

17177539 .. 17188836

Gene Structure 11298 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G41220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4890 bp

ATGGCTCTACAGTCTCCCGGAGCTACCGGAGCTTCATCTTCCGTTTCCCGGCTTCTCTCTTCCGCGAAATTAAGCTCTACTAAGACTATCTTCTCTGTTGACTTCGTCAGATCCTACTGTATTTCTAAAGGAACCAAACGGCGTAACGAACTCTCCGGCTTTCGCGGCTACAGTCCACTGCTCAAGTCCTCGCTGAGGTCTCCGTTTTCGGTGAAAGCGATCCTTAATTCTGACCGAGCCGCTGGTGATGCATCGTCTTCCTTTTCTGATCTGAAACCTCAGGTGGCTTATTTGGAAGATATAATATCGGAAAGGGGAGCATGTGGTGTTGGGTTTATTGCAAACTTGGAGAATAAGGCTACACATAAGATTGTAAATGATGCTCTTATAGCACTTGGTTGTATGGAACACAGGGGAGGTTGTGGTTCTGATAATACTTCTGGTGATGGTTCTGGTTTGATGACTTCCATTCCTTGGGATCTATTTAACGAATGGGCTGAGAAGCAAGGAATCGCTTCTTTTGATAGGACGCATACGGGTGTTGGAATGCTGTTCCTACCGAGAGATGACAACATTAGGAAAGAAGCCAAGAAAGTGATTACAAGTATTTTTGAGAAAGAGGGCCTGGAGGTGCTTGGATGGAGAGATGTTCCTGTGGAAGCGTCCATTGTTGGCCATAATGCAAAACAGACGATGCCAAACACGGAACAGGTCTTTGTTAGAATTGTCAAAGACGATAAAGTAGACGACGTTGAACGAGAACTTTACATTTGCAGGAAGCTGATCGAAAGAGCAGTTGCTTCTGAAAGTTGGGCCTCTGAGCTCTACTTTAGCTCCTTGTCCAATCAGACCATTGTTTACAAGGGAATGCTTCGTTCTGAAGTTCTTGGGTTATTTTACCCCGACCTTCAAAATGATCTTTATAAATCTCCTTTCGCCATCTATCATCGAAGATTTAGCACAAATACTAGTCCCAGATGGCATCTTGCTCAGCCCATGAGGTTTCTTGGGCACAATGGGGAGATCAATACCATACAGGGGAACCTAAACTGGATGACGTCTCGAGAAGCATCTCTAAGATCACCTGTCTGGCATGGACGTGAAAATGACATTCGTCCAATTAGCAATCCAAAGGCTTCAGACTCTGCTAACCTTGACAGTGCGGCGGAGCTATTGATAAGAAGTGGCCGGACTCCAGAGGAATCTCTTATGATTCTTGTCCCAGAGGCGTATAAAAATCACCCGACTTTAATGATTAAATACCCTGAGGCTGTAGACTTCTATGACTATTACAAGGGGCAGATGGAGCCCTGGGATGGACCTGCTTTGGTATTGTTCAGTGATGGAAAGACTGTTGGAGCTTGCCTTGACCGCAATGGACTTCGACCAGCTAGATATTGGCGGACAAGTGATAATGTTGTCTATGTAGCCTCTGAGGTCGGAGTTCTTCCAATGGATGAATCGAAAGTCACCATGAAGGGTCGTCTAGGACCTGGTATGATGATATCTGTTGACTTAGAGAATGGACAGGTATATGAGAACACAGAAGTGAAGAAGCGGGTTGCATCATATAACCCATATGGAAAGTGGGTTAGTGAAAACCTGCGAAACCTGAAGCCTTCTAATTATCTCTCGTCAGCGATCCTGGAGACTGATGAGACCTTAAGACGCCAACAGGCGTTTGGCTACTCGAGTGAAGATGTTCAAATGGTAATTGAGTCGATGGCTGCACAAGGAAAAGAACCAACGTTTTGCATGGGGGATGATACTCCAGTGGCAGTATTGTCTCAAAAGCCACATATGCTTTATGATTATTTCAAGCAGCGGTTTGCTCAGGTTACAAATCCAGCTATTGACCCTCTCCGGGAAGGATTAGTCATGTCCCTTGAAGTTAATATTGGAAAGCGTGGAAATATATTGGAGGTTGGGCCTCAGAATGTTTCACAGGTTGTTTTGTCTGGTCCTGTGCTAAATGAACGGGAGCTTGAGGGTTTGCTTGGTGATCCACTGTTAAAATCTCAAATCTTGCCCACATTTTTTGACATTCGTAGAGGAATTGAAGGATCTTTGAAGAAGGGTCTTCTAAAACTTTGTGAAGCTGCAGATGAAGCTGTTCGTAATGGTTCCCAAGTACTTGTTCTCTCAGACAGATCTGATAATCCGGAACCAACTCGACCTGCAATTCCAATGTTGTTGGCTGTGGGTGCTGTTCACCAACATCTGATCCAGAACGGTCTTCGGATGTCAGCTTCGATAATTGCAGATACAGCTCAGTGCTTTAGTACACATCACTTTGCCTGTTTGATTGGATATGGAGCAAGTGCTATATGCCCGCACCTAGCACTGGAGACGTGTAGACAGTGGCGTCTGAGCAACAAGACCGTGAACATGATGAGAAACGGAAAAATGCCTACTGTAACTATGGAACAGGCTCAGAAGAATTACCGCAAGGCTGTTAACACTGGTCTTCTTAAGGTTCTCTCTAAGATGGGCATCTCGTTATTCTCTAGTTATTGTGGAGCACAAATATTTGAAATTTATGGATTGGGAAATGAGGTTGTGGAATTTTCGTTCCGTGGTAGTGCATCTCAAATTGGTGGATTAACTTTAGATGAGCTGGCTAGGGAGACGTTAACTTTCTGGGTGAGGGCATTTTCTGAGGATACAGCCAAACGGCTAGAGAACTTCGGTTTCATTCAGTTTAGGCCTGGAGGTGAATATCATGGAAACAACCCAGAAATGTCTAAATTACTTCATAAAGCCGTCCGCGAAAAGAGTGAGACAGCCTATGCAGTCTATCAGCAGCATTTAGCCAACCGTCCTATTACGGTTTTCCGCGATCTTCTTGAGTTCAAAAGTGACCGTAACCCTATCCCTGTTGGGAAGGTTGAGCCTGCTTCCTCTATCGTGGAACGGTTTTGTACAGGTGGAATGTCTCTTGGAGCAATCTCAAGAGAAACTCATGAGACAATTGCTATTGCAATGAACAGATTAGGAGGAAAATCCAACTCAGGGGAAGGAGGCGAGGATCCGATCCGCTGGAAGCCGCTAACAGATGTTGTTGATGGGTATTCTTCAACTCTGCCACATCTTAAAGGTCTTCGAAATGGGGATACGGCTACAAGTGCTATTAAGCAGGTTGCCTCAGGGCGTTTTGGTGTCACTCCGACGTTTTTGGTTAATGCGGACCAGTTGGAAATTAAAGTTGCTCAAGGGGCGAAGCCAGGTGAAGGTGGCCAACTTCCTGGGAAAAAAGTCAGCGCATATATTGCTAGACTTAGAAATTCTAAACCAGGAGTTCCGCTTATTTCTCCACCTCCACATCATGATATTTATTCCATAGAGGATCTGGCACAGTTGATCTTTGATCTCCATCAGGTCAACCCCAAGGCAAAAGTGTCAGTCAAGCTTGTATCAGAAACTGGAATAGGAACAGTTGCCTCCGGAGTAGCGAAGGCTAATGCCGATATCATACAGATATCAGGATATGATGGTGGAACTGGAGCCAGCCCTATAAGTTCCATTAAGCATGCTGGTGGCCCGTGGGAGCTGGGACTAGCTGAAACCCAGAAGACTCTCATTGGAAATGGACTAAGGGAAAGAGTTATTATCAGGGTTGATGGCGGATTCAAAAGTGGTGTGGATGTGTTGATAGCTGCAGCTATGGGCGCTGATGAGTATGGTTTTGGTACTCTGGCAATGATTGCCACAGGATGTATCATGGCTCGCATTTGCCACACTAATAACTGCCCCGTTGGTGTTGCCAGTCAGAGAGAGGAACTGCGTGCACGTTTCCCCGGTCTTCCTGGTGATCTTGTCAACTTCTTCTTGTATATTGCAGAGGAGGTGAGGGGCATATTAGCTCAGCTGGGATATGAAAAGTTGGATGATATAATTGGGCGGACAGATTTGCTAAAGGCTAGGGATATCTCGCTCGTGAAAACTCATCTCGACCTCAGTTATCTCTTATCTTCTGTTGGGCTACCAAAACGAAGCAGTACTTCCATTAGGAAGCAGGAGGTTCATTCAAATGGTCCTGTTCTTGATGATACTCTACTTCAGGATCCAGAGATAATGGATGCAATTGAGAATGAAAAGACGGTTCACAAAACCATGAGTATATACAATGTAGATCGTTCTGTTTGCGGTCGGATTGCTGGGGTGATTGCGAAGAAATACGGAGACACTGGTTTTGCTGGACAACTGAACCTAACGTTCACTGGAAGTGCTGGCCAATCTTTTGCATGTTTCCTAACTCCTGGAATGAATATACGTCTTGTGGGTGAAGCCAACGACTATGTGGGAAAGGGAATGGCGGGAGGTGAAGTTGTGATATTACCAGTGGAATCGACTGGTTTTCGTCCCGAAGACGCAACTATTGTAGGAAACACTTGTCTGTATGGTGCAACTGGTGGTTTATTATTTGTCAGAGGCAAAGCAGGGGAGAGATTTGCGGTTAGAAACTCTCTAGCTCAAGCTGTTGTTGAAGGCACTGGAGATCATTGCTGTGAATATATGACTGGTGGTTGTGTCGTTATACTTGGGAAAGTTGGTAGAAATGTAGCCGCTGGGATGACAGGTGGATTGGCATATATCCTTGACGAAGACAACACTCTCCTCCCTAAGATGAACAAAGAGATAGTGAAGATCCAAAGAGTGACTTCACCAGTGGGACAAACACAGCTTAAAAGCCTGATCCAGGCTCATGTGGAGAAAACGGGAAGCAGCAAAGGGGCAATGATTGTGGAAGAGTGGGATAAGTATCTAGCAATGTTCTGGCAACTTGTACCTCCGAGTGAAGAAGACACACCTGAGGCTAACTCAGACCACATCCTGAAAACAACCACAGGAGATGAAGAACAGGTTTCAAGCACATTAGCAGAGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009853 GO:0009941 GO:0009987 GO:0010033 GO:0015930 GO:0016020 GO:0016041 GO:0016053 GO:0016491 GO:0016638 GO:0016643 GO:0019222 GO:0019676 GO:0019740 GO:0019752 GO:0019899 GO:0031323 GO:0031967 GO:0031974 GO:0031975 GO:0034285 GO:0042221 GO:0043085 GO:0043094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0043650 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048046 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051338 GO:0051347 GO:0055114 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0080114 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00630 ko00910 ko01120 map00630 map00910 map01120

Pfam Domains

Protein Families

GATase_2 GXGXG Glu_syn_central Glu_synthase

Protein Analysis

1629

Amino Acids

177.75

Weight (kDa)

6.57

Isoelectric Point (pI)

36.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	108 - 530	5.8e-182	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	556 - 849	1.7e-105	Glutamate synthase central domain
Glu_synthase	PF01645	909 - 1292	2.9e-154	Conserved region in glutamate synthase
GXGXG	PF01493	1373 - 1556	3e-78	GXGXG motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005346)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41220 AT5G04140 AT5G04140
fragaria_vesca	FvH4_6g25460 FvH4_6g25460 FvH4_6g25460 FvH4_6g25460
malus_domestica	MD14G1087500.v1.1
prunus_persica	Prupe.7G039100_v2.0.a1
pyrus_communis	pycom14g07760 pycom14g07770
rosa_chinensis	RchiOBHm_Chr2g0125471
rosa_laevigata	RLG00000018438 RLG00000018867 RLG00000030615 RLG00000034746
rosa_multiflora	Rmu_sc0005339.1_g000009 Rmu_sc0005339.1_g000011 Rmu_ssc0000126.1_g000014
rosa_roxburghii	Rroxscaffold_1G00035740 Rroxscaffold_2G00117960
rosa_rugosa	Rorug02G0257500 Rorug02G0257600
rosa_samantha	Rh2AG316500 Rh2BG324000 Rh2BG355400 Rh2CG306200 Rh2DG342600
rosa_wichuraiana	Rw2G025530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	924
AasI	GACNNNNNNGTC	1 cut(s)	2364
AatII	GACGTC	1 cut(s)	1061
Acc36I	ACCTGC	4 cut(s)	1330, 1611, 2185, 3127
AccB7I	CCANNNNNTGG	1 cut(s)	474
AccI	GTMKAC	3 cut(s)	744, 1275, 2356
AccII	CGCG	4 cut(s)	65, 165, 2769, 2838
AccIII	TCCGGA	2 cut(s)	2161, 3443
AclI	AACGTT	2 cut(s)	1748, 4214
AcoI	YGGCCR	4 cut(s)	673, 1186, 3235, 4231
AcsI	RAATTY	3 cut(s)	2535, 2564, 3283
AcuI	CTGAAG	4 cut(s)	900, 1122, 1634, 4043
AcyI	GRCGYC	3 cut(s)	1058, 1669, 2365
AfaI	GTAC	6 cut(s)	2133, 2284, 2923, 3691, 4000, 4788
AflII	CTTAAG	2 cut(s)	1663, 2471
AflIII	ACRYGT	1 cut(s)	2351
AhlI	ACTAGT	1 cut(s)	968
AjiI	CACGTC	2 cut(s)	1100, 2352
AjuI	GAANNNNNNNTTGG	4 cut(s)	1881, 1913, 2121, 2153
AleI	CACNNNNGTG	1 cut(s)	1547
AloI	GAACNNNNNNTCC	2 cut(s)	2555, 2587
Alw21I	GWGCWC	3 cut(s)	825, 2524, 3790
Alw44I	GTGCAC	1 cut(s)	3786
AlwNI	CAGNNNCTG	2 cut(s)	1145, 3422
Ama87I	CYCGRG	2 cut(s)	1062, 1690
Aor13HI	TCCGGA	2 cut(s)	2161, 3443
AoxI	GGCC	9 cut(s)	622, 673, 813, 1186, 1928, 2705, 3235, 3538, 4231
ApaLI	GTGCAC	1 cut(s)	3786
ApeKI	GCWGC	7 cut(s)	1727, 1822, 2099, 2803, 3656, 3659, 4725
ApoI	RAATTY	3 cut(s)	2535, 2564, 3283
ArsI	GACNNNNNNTTYG	2 cut(s)	4175, 4207
Asp700I	GAANNNNTTC	3 cut(s)	514, 1701, 2112
AspLEI	GCGC	2 cut(s)	3263, 3671
AspS9I	GGNCC	9 cut(s)	622, 813, 1319, 1490, 1928, 1961, 3187, 3539, 4034
AsuC2I	CCSGG	4 cut(s)	18, 49, 1865, 3798
AsuII	TTCGAA	1 cut(s)	3103
AvaI	CYCGRG	2 cut(s)	1062, 1690
AvaII	GGWCC	5 cut(s)	1319, 1490, 1961, 3187, 4034
AxyI	CCTNAGG	4 cut(s)	279, 1266, 3145, 4812
BaeGI	GKGCMC	2 cut(s)	1014, 3790
BaeI	ACNNNNGTAYC	2 cut(s)	1758, 1791
BalI	TGGCCA	3 cut(s)	675, 3237, 4233
BamHI	GGATCC	2 cut(s)	3028, 4063
BanII	GRGCYC	2 cut(s)	825, 1311
BauI	CACGAG	1 cut(s)	3935
Bbv12I	GWGCWC	3 cut(s)	825, 2524, 3790
BbvCI	CCTCAGC	1 cut(s)	194
BbvI	GCAGC	7 cut(s)	1714, 1834, 2086, 2815, 3643, 3671, 4737
BceAI	ACGGC	4 cut(s)	155, 2690, 2747, 3132
BciVI	GTATCC	2 cut(s)	2656, 3106
BcnI	CCSGG	4 cut(s)	18, 49, 1865, 3798
BcuI	ACTAGT	1 cut(s)	968
BfmI	CTRYAG	5 cut(s)	8, 169, 1272, 2100, 3657
BfoI	RGCGCY	1 cut(s)	3672
BfrI	CTTAAG	2 cut(s)	1663, 2471
BfuAI	ACCTGC	4 cut(s)	1330, 1611, 2185, 3127
BfuI	GTATCC	2 cut(s)	2656, 3106
BglII	AGATCT	1 cut(s)	2150
BlpI	GCTNAGC	2 cut(s)	990, 3864
BmcAI	AGTACT	2 cut(s)	2133, 4000
Bme18I	GGWCC	5 cut(s)	1319, 1490, 1961, 3187, 4034
BmeT110I	CYCGRG	2 cut(s)	1062, 1690
BmgBI	CACGTC	2 cut(s)	1100, 2352
BmgT120I	GGNCC	9 cut(s)	622, 813, 1319, 1490, 1928, 1961, 3187, 3539, 4034
BmiI	GGNNCC	8 cut(s)	133, 1043, 1308, 2125, 2166, 3030, 3505, 4065
BoxI	GACNNNNGTC	1 cut(s)	1113
BplI	GAGNNNNNCTC	2 cut(s)	4805, 4837
BpmI	CTGGAG	8 cut(s)	647, 1179, 1667, 1755, 2366, 2730, 3522, 4515
Bpu10I	CCTNAGC	2 cut(s)	194, 1833
Bpu1102I	GCTNAGC	2 cut(s)	990, 3864
Bpu14I	TTCGAA	1 cut(s)	3103
BpuEI	CTTGAG	6 cut(s)	167, 2006, 2867, 2935, 3195, 4458
BpuMI	CCSGG	4 cut(s)	18, 49, 1865, 3798
BsaBI	GATNNNNATC	3 cut(s)	2492, 3372, 4761
BsaHI	GRCGYC	3 cut(s)	1058, 1669, 2365
BsaI	GGTCTC	2 cut(s)	204, 1652
BsaJI	CCNNGG	8 cut(s)	473, 1310, 1311, 2575, 3245, 3387, 3542, 3796
BsaWI	WCCGGW	3 cut(s)	26, 2161, 3443
BsaXI	ACNNNNNCTCC	6 cut(s)	137, 167, 818, 848, 4311, 4341
Bse21I	CCTNAGG	4 cut(s)	279, 1266, 3145, 4812
Bse3DI	GCAATG	6 cut(s)	1381, 2988, 3705, 4501, 4743, 4776
Bse8I	GATNNNNATC	3 cut(s)	2492, 3372, 4761
BseAI	TCCGGA	2 cut(s)	2161, 3443
BseDI	CCNNGG	8 cut(s)	473, 1310, 1311, 2575, 3245, 3387, 3542, 3796
BseJI	GATNNNNATC	3 cut(s)	2492, 3372, 4761
BseMI	GCAATG	6 cut(s)	1381, 2988, 3705, 4501, 4743, 4776
BseRI	GAGGAG	2 cut(s)	3857, 4607
BseSI	GKGCMC	2 cut(s)	1014, 3790
BseXI	GCAGC	7 cut(s)	1714, 1834, 2086, 2815, 3643, 3671, 4737
BseYI	CCCAGC	4 cut(s)	3550, 3868, 4157, 4568
BsgI	GTGCAG	1 cut(s)	1713
Bsh1236I	CGCG	4 cut(s)	65, 165, 2769, 2838
Bsh1285I	CGRYCG	1 cut(s)	4150
BshFI	GGCC	9 cut(s)	624, 675, 815, 1188, 1930, 2707, 3237, 3540, 4233
BsiEI	CGRYCG	1 cut(s)	4150
BsiHKAI	GWGCWC	3 cut(s)	825, 2524, 3790
BsiHKCI	CYCGRG	2 cut(s)	1062, 1690
BsiSI	CCGG	9 cut(s)	18, 27, 49, 156, 1189, 1864, 2162, 3444, 3798
BslFI	GGGAC	6 cut(s)	957, 1205, 1867, 3567, 4347, 4686
BsmBI	CGTCTC	3 cut(s)	1065, 2342, 2621
BsmFI	GGGAC	6 cut(s)	957, 1205, 1867, 3567, 4347, 4686
BsmI	GAATGC	2 cut(s)	552, 873
BsnI	GGCC	9 cut(s)	624, 675, 815, 1188, 1930, 2707, 3237, 3540, 4233
Bso31I	GGTCTC	2 cut(s)	204, 1652
BsoBI	CYCGRG	2 cut(s)	1062, 1690
Bsp119I	TTCGAA	1 cut(s)	3103
Bsp1286I	GDGCHC	5 cut(s)	825, 1014, 1311, 2524, 3790
Bsp13I	TCCGGA	2 cut(s)	2161, 3443
Bsp1407I	TGTACA	1 cut(s)	2921
Bsp1720I	GCTNAGC	2 cut(s)	990, 3864
BspANI	GGCC	9 cut(s)	624, 675, 815, 1188, 1930, 2707, 3237, 3540, 4233
BspEI	TCCGGA	2 cut(s)	2161, 3443
BspFNI	CGCG	4 cut(s)	65, 165, 2769, 2838
BspHI	TCATGA	2 cut(s)	2962, 3325
BspLI	GGNNCC	8 cut(s)	133, 1043, 1308, 2125, 2166, 3030, 3505, 4065
BspMAI	CTGCAG	2 cut(s)	2104, 3661
BspMI	ACCTGC	4 cut(s)	1330, 1611, 2185, 3127
BspT104I	TTCGAA	1 cut(s)	3103
BspTI	CTTAAG	2 cut(s)	1663, 2471
BspTNI	GGTCTC	2 cut(s)	204, 1652
BsrDI	GCAATG	6 cut(s)	1381, 2988, 3705, 4501, 4743, 4776
BsrGI	TGTACA	1 cut(s)	2921
BssECI	CCNNGG	8 cut(s)	473, 1310, 1311, 2575, 3245, 3387, 3542, 3796
BssNI	GRCGYC	3 cut(s)	1058, 1669, 2365
BssSI	CACGAG	1 cut(s)	3935
BssT1I	CCWWGG	2 cut(s)	473, 3387
Bst2BI	CACGAG	1 cut(s)	3935
Bst6I	CTCTTC	2 cut(s)	64, 4743
BstACI	GRCGYC	3 cut(s)	1058, 1669, 2365
BstAFI	CTTAAG	2 cut(s)	1663, 2471
BstAPI	GCANNNNNTGC	2 cut(s)	3269, 3704
BstAUI	TGTACA	1 cut(s)	2921
BstBI	TTCGAA	1 cut(s)	3103
BstC8I	GCNNGC	9 cut(s)	1363, 2333, 2619, 2895, 3508, 3531, 3727, 3786, 4231
BstDSI	CCRYGG	2 cut(s)	2575, 3542
BstENI	CCTNNNNNAGG	2 cut(s)	193, 4697
BstFNI	CGCG	4 cut(s)	65, 165, 2769, 2838
BstH2I	RGCGCY	1 cut(s)	3672
BstHHI	GCGC	2 cut(s)	3263, 3671
BstMCI	CGRYCG	1 cut(s)	4150
BstNSI	RCATGY	3 cut(s)	324, 3533, 4248
BstPAI	GACNNNNGTC	1 cut(s)	1113
BstSFI	CTRYAG	5 cut(s)	8, 169, 1272, 2100, 3657
BstSLI	GKGCMC	2 cut(s)	1014, 3790
BstUI	CGCG	4 cut(s)	65, 165, 2769, 2838
BstV1I	GCAGC	7 cut(s)	1714, 1834, 2086, 2815, 3643, 3671, 4737
BstX2I	RGATCY	8 cut(s)	110, 478, 2063, 2150, 3028, 3346, 4063, 4647
BstXI	CCANNNNNNTGG	1 cut(s)	3245
BstYI	RGATCY	8 cut(s)	110, 478, 2063, 2150, 3028, 3346, 4063, 4647
Bsu36I	CCTNAGG	4 cut(s)	279, 1266, 3145, 4812
BsuI	GTATCC	2 cut(s)	2656, 3106
BsuRI	GGCC	9 cut(s)	624, 675, 815, 1188, 1930, 2707, 3237, 3540, 4233
BtgI	CCRYGG	2 cut(s)	2575, 3542
BtrI	CACGTC	2 cut(s)	1100, 2352
BtsI	GCAGTG	1 cut(s)	176
BveI	ACCTGC	4 cut(s)	1330, 1611, 2185, 3127
Cac8I	GCNNGC	9 cut(s)	1363, 2333, 2619, 2895, 3508, 3531, 3727, 3786, 4231
CaiI	CAGNNNCTG	2 cut(s)	1145, 3422
CciI	TCATGA	2 cut(s)	2962, 3325
CfoI	GCGC	2 cut(s)	3263, 3671
Cfr13I	GGNCC	9 cut(s)	622, 813, 1319, 1490, 1928, 1961, 3187, 3539, 4034
CseI	GACGC	5 cut(s)	538, 651, 1677, 2354, 4379
CsiI	ACCWGGT	1 cut(s)	1492
Csp6I	GTAC	6 cut(s)	2132, 2283, 2922, 3690, 3999, 4787
CspCI	CAANNNNNGTGG	4 cut(s)	2020, 2055, 4725, 4760
CviQI	GTAC	6 cut(s)	2132, 2283, 2922, 3690, 3999, 4787
DrdI	GACNNNNNNGTC	1 cut(s)	2364
DseDI	GACNNNNNNGTC	1 cut(s)	2364
EaeI	YGGCCR	4 cut(s)	673, 1186, 3235, 4231
Eam1104I	CTCTTC	2 cut(s)	64, 4743
EarI	CTCTTC	2 cut(s)	64, 4743
EciI	GGCGGA	4 cut(s)	1181, 1417, 3639, 3916
Ecl136II	GAGCTC	1 cut(s)	823
Eco130I	CCWWGG	2 cut(s)	473, 3387
Eco147I	AGGCCT	1 cut(s)	2707
Eco24I	GRGCYC	2 cut(s)	825, 1311
Eco31I	GGTCTC	2 cut(s)	204, 1652
Eco32I	GATATC	4 cut(s)	1506, 3469, 3480, 3928
Eco47I	GGWCC	5 cut(s)	1319, 1490, 1961, 3187, 4034
Eco53kI	GAGCTC	1 cut(s)	823
Eco57I	CTGAAG	4 cut(s)	900, 1122, 1634, 4043
Eco81I	CCTNAGG	4 cut(s)	279, 1266, 3145, 4812
Eco88I	CYCGRG	2 cut(s)	1062, 1690
EcoICRI	GAGCTC	1 cut(s)	823
EcoNI	CCTNNNNNAGG	2 cut(s)	193, 4697
EcoO109I	RGGNCCY	2 cut(s)	622, 1490
EcoRV	GATATC	4 cut(s)	1506, 3469, 3480, 3928
EcoT14I	CCWWGG	2 cut(s)	473, 3387
EcoT22I	ATGCAT	1 cut(s)	253
EcoT38I	GRGCYC	2 cut(s)	825, 1311
ErhI	CCWWGG	2 cut(s)	473, 3387
Esp3I	CGTCTC	3 cut(s)	1065, 2342, 2621
FalI	AAGNNNNNCTT	8 cut(s)	74, 106, 207, 239, 3985, 4017, 4645, 4677
FaqI	GGGAC	6 cut(s)	957, 1205, 1867, 3567, 4347, 4686
FauI	CCCGC	3 cut(s)	1551, 2340, 4309
FauNDI	CATATG	2 cut(s)	1579, 1800
FblI	GTMKAC	3 cut(s)	744, 1275, 2356
FriOI	GRGCYC	2 cut(s)	825, 1311
GlaI	GCGC	2 cut(s)	3262, 3670
GsaI	CCCAGC	4 cut(s)	3554, 3872, 4161, 4572
GsuI	CTGGAG	8 cut(s)	647, 1179, 1667, 1755, 2366, 2730, 3522, 4515
HaeII	RGCGCY	1 cut(s)	3672
HaeIII	GGCC	9 cut(s)	624, 675, 815, 1188, 1930, 2707, 3237, 3540, 4233
HapII	CCGG	9 cut(s)	18, 27, 49, 156, 1189, 1864, 2162, 3444, 3798
HgaI	GACGC	5 cut(s)	538, 651, 1677, 2354, 4379
HhaI	GCGC	2 cut(s)	3263, 3671
Hin1I	GRCGYC	3 cut(s)	1058, 1669, 2365
Hin6I	GCGC	2 cut(s)	3261, 3669
HinP1I	GCGC	2 cut(s)	3261, 3669
HincII	GTYRAC	6 cut(s)	100, 1513, 2458, 2634, 3382, 3820
HindII	GTYRAC	6 cut(s)	100, 1513, 2458, 2634, 3382, 3820
HindIII	AAGCTT	1 cut(s)	3407
HpaI	GTTAAC	2 cut(s)	2458, 2634
HpaII	CCGG	9 cut(s)	18, 27, 49, 156, 1189, 1864, 2162, 3444, 3798
Hpy99I	CGWCG	2 cut(s)	752, 3172
Hsp92I	GRCGYC	3 cut(s)	1058, 1669, 2365
HspAI	GCGC	2 cut(s)	3261, 3669
Kpn2I	TCCGGA	2 cut(s)	2161, 3443
KspAI	GTTAAC	2 cut(s)	2458, 2634
Lsp1109I	GCAGC	7 cut(s)	1714, 1834, 2086, 2815, 3643, 3671, 4737
MabI	ACCWGGT	1 cut(s)	1492
MaeIII	GTNAC	8 cut(s)	143, 1468, 1837, 2416, 2858, 2864, 3160, 4657
MfeI	CAATTG	2 cut(s)	2970, 4083
MflI	RGATCY	8 cut(s)	110, 478, 2063, 2150, 3028, 3346, 4063, 4647
MhlI	GDGCHC	5 cut(s)	825, 1014, 1311, 2524, 3790
MlsI	TGGCCA	3 cut(s)	675, 3237, 4233
MluNI	TGGCCA	3 cut(s)	675, 3237, 4233
MlyI	GAGTC	4 cut(s)	1136, 1186, 1727, 3568
MmeI	TCCRAC	8 cut(s)	523, 1336, 1420, 3030, 3174, 3191, 3864, 4130
Mox20I	TGGCCA	3 cut(s)	675, 3237, 4233
Mph1103I	ATGCAT	1 cut(s)	253
MroI	TCCGGA	2 cut(s)	2161, 3443
MroXI	GAANNNNTTC	3 cut(s)	514, 1701, 2112
MscI	TGGCCA	3 cut(s)	675, 3237, 4233
MslI	CAYNNNNRTG	6 cut(s)	537, 1098, 1547, 3534, 4029, 4506
Msp20I	TGGCCA	3 cut(s)	675, 3237, 4233
MspA1I	CMGCKG	5 cut(s)	242, 1825, 3039, 3868, 4568
MspCI	CTTAAG	2 cut(s)	1663, 2471
MspI	CCGG	9 cut(s)	18, 27, 49, 156, 1189, 1864, 2162, 3444, 3798
MunI	CAATTG	2 cut(s)	2970, 4083
Mva1269I	GAATGC	2 cut(s)	552, 873
MvnI	CGCG	4 cut(s)	65, 165, 2769, 2838
NciI	CCSGG	4 cut(s)	18, 49, 1865, 3798
NdeI	CATATG	2 cut(s)	1579, 1800
NlaIV	GGNNCC	8 cut(s)	133, 1043, 1308, 2125, 2166, 3030, 3505, 4065
NmuCI	GTSAC	4 cut(s)	1468, 2858, 3160, 4657
NsiI	ATGCAT	1 cut(s)	253
NspI	RCATGY	3 cut(s)	324, 3533, 4248
NspV	TTCGAA	1 cut(s)	3103
OliI	CACNNNNGTG	1 cut(s)	1547
PaeI	GCATGC	1 cut(s)	3533
PaeR7I	CTCGAG	2 cut(s)	1062, 1690
PagI	TCATGA	2 cut(s)	2962, 3325
PasI	CCCWGGG	1 cut(s)	1311
PceI	AGGCCT	1 cut(s)	2707
PcsI	WCGNNNNNNNCGW	1 cut(s)	215
PctI	GAATGC	2 cut(s)	552, 873
PdmI	GAANNNNTTC	3 cut(s)	514, 1701, 2112
PfeI	GAWTC	6 cut(s)	510, 1202, 1213, 1460, 3627, 4346
PflFI	GACNNNGTC	1 cut(s)	104
PflMI	CCANNNNNTGG	1 cut(s)	474
PfoI	TCCNGGA	4 cut(s)	16, 1644, 1863, 4258
PleI	GAGTC	4 cut(s)	1136, 1186, 1726, 3568
PpsI	GAGTC	4 cut(s)	1136, 1186, 1726, 3568
PpuMI	RGGWCCY	1 cut(s)	1490
PshAI	GACNNNNGTC	1 cut(s)	1113
PsiI	TTATAA	1 cut(s)	924
Psp124BI	GAGCTC	1 cut(s)	825
Psp1406I	AACGTT	2 cut(s)	1748, 4214
Psp5II	RGGWCCY	1 cut(s)	1490
PspFI	CCCAGC	4 cut(s)	3550, 3868, 4157, 4568
PspN4I	GGNNCC	8 cut(s)	133, 1043, 1308, 2125, 2166, 3030, 3505, 4065
PspPI	GGNCC	9 cut(s)	622, 813, 1319, 1490, 1928, 1961, 3187, 3539, 4034
PspPPI	RGGWCCY	1 cut(s)	1490
PspXI	VCTCGAGB	1 cut(s)	1690
PstI	CTGCAG	2 cut(s)	2104, 3661
PstNI	CAGNNNCTG	2 cut(s)	1145, 3422
PsuI	RGATCY	8 cut(s)	110, 478, 2063, 2150, 3028, 3346, 4063, 4647
PsyI	GACNNNGTC	1 cut(s)	104
PvuII	CAGCTG	1 cut(s)	3868
RsaI	GTAC	6 cut(s)	2133, 2284, 2923, 3691, 4000, 4788
RsaNI	GTAC	6 cut(s)	2132, 2283, 2922, 3690, 3999, 4787
RseI	CAYNNNNRTG	6 cut(s)	537, 1098, 1547, 3534, 4029, 4506
SacI	GAGCTC	1 cut(s)	825
Sau96I	GGNCC	9 cut(s)	622, 813, 1319, 1490, 1928, 1961, 3187, 3539, 4034
ScaI	AGTACT	2 cut(s)	2133, 4000
SchI	GAGTC	4 cut(s)	1136, 1186, 1727, 3568
SduI	GDGCHC	5 cut(s)	825, 1014, 1311, 2524, 3790
SexAI	ACCWGGT	1 cut(s)	1492
SfcI	CTRYAG	5 cut(s)	8, 169, 1272, 2100, 3657
Sfr274I	CTCGAG	2 cut(s)	1062, 1690
SfuI	TTCGAA	1 cut(s)	3103
SinI	GGWCC	5 cut(s)	1319, 1490, 1961, 3187, 4034
SlaI	CTCGAG	2 cut(s)	1062, 1690
SmiMI	CAYNNNNRTG	6 cut(s)	537, 1098, 1547, 3534, 4029, 4506
SpeI	ACTAGT	1 cut(s)	968
SphI	GCATGC	1 cut(s)	3533
SseBI	AGGCCT	1 cut(s)	2707
SspI	AATATT	2 cut(s)	1897, 2529
SstI	GAGCTC	1 cut(s)	825
StuI	AGGCCT	1 cut(s)	2707
StyI	CCWWGG	2 cut(s)	473, 3387
TaqII	GACCGA	1 cut(s)	249
TatI	WGTACW	4 cut(s)	2131, 2282, 2921, 3998
TauI	GCSGC	5 cut(s)	168, 242, 1166, 3049, 4568
TfiI	GAWTC	6 cut(s)	510, 1202, 1213, 1460, 3627, 4346
TseFI	GTSAC	4 cut(s)	1468, 2858, 3160, 4657
TseI	GCWGC	7 cut(s)	1727, 1822, 2099, 2803, 3656, 3659, 4725
Tsp45I	GTSAC	4 cut(s)	1468, 2858, 3160, 4657
TspGWI	ACGGA	6 cut(s)	31, 192, 719, 2415, 2564, 4194
Tth111I	GACNNNGTC	1 cut(s)	104
Van91I	CCANNNNNTGG	1 cut(s)	474
Vha464I	CTTAAG	2 cut(s)	1663, 2471
VneI	GTGCAC	1 cut(s)	3786
VpaK11BI	GGWCC	5 cut(s)	1319, 1490, 1961, 3187, 4034
XagI	CCTNNNNNAGG	2 cut(s)	193, 4697
XapI	RAATTY	3 cut(s)	2535, 2564, 3283
XceI	RCATGY	3 cut(s)	324, 3533, 4248
XcmI	CCANNNNNNNNNTGG	5 cut(s)	1395, 1584, 1679, 3346, 4297
XhoI	CTCGAG	2 cut(s)	1062, 1690
XmiI	GTMKAC	3 cut(s)	744, 1275, 2356
XmnI	GAANNNNTTC	3 cut(s)	514, 1701, 2112
ZraI	GACGTC	1 cut(s)	1059
ZrmI	AGTACT	2 cut(s)	2133, 4000
Zsp2I	ATGCAT	1 cut(s)	253

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.