Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005346

Physical Location & Seq

Genomic Coordinates Forward (+)

1129785 .. 1138488

Gene Structure 8704 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G04140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4869 bp

ATGGCGATGCAATCTCTTTCCCCTGTTCCTAAGCTTCTCTCCACAACACCAAGCTCTGTTCTTTCTTCTGACAAGAACTTCTTCTTCGTCGATTTCGTTGGATTATACTGTAAGTCCAAGAGGACCAGACGCAGACTTCGTGGAGACTCTTCCTCTTCCTCACGCTCTTCTTCTTCTCTCTCTCGTCTTTCCTCTGTTCGTGCCGTTATCGACCTTGAACGTGTTCATGGCGTCTCTGAAAAGGATCTCTCCTCTCCTTCAGCTTTAAGACCTCAGGTTGCTAATTTGGAGGATATATTGTCTGAAAGAGGAGCTTGTGGAGTTGGGTTTATAGCAAACTTAGATAACATACCTTCACATGGAGTTGTCAAAGATGCTCTTATTGCTCTTGGGTGTATGGAACATCGTGGAGGTTGTGGAGCAGACAATGATTCTGGTGATGGCTCTGGTCTTATGTCTTCCATTCCTTGGGATTTCTTTAACGTCTGGGCCAAGGAACAAAGTCTTGCTCCTTTTGATAAGTTGCATACTGGTGTTGGCATGATCTTTCTTCCACAAGATGATACCTTTATGCAAGAAGCCAAGCAAGTTATTGAAAACATATTTGAGAAAGAAGGATTACAAGTTCTTGGGTGGAGGGAAGTTCCTGTTAATGTTCCTATAGTTGGTAAAAATGCTAGGGAGACAATGCCTAACATTCAACAAGTGTTTGTGAAAATCGCAAAGGAAGATAGTACTGATGATATTGAAAGGGAACTTTACATCTGCCGGAAACTAATCGAAAGGGCCGTAGCTACTGAGAGTTGGGGAACAGAGCTTTACTTCTGTTCACTGTCCAATCAAACCATAGTGTACAAGGGCATGCTTCGATCTGAAGCTCTTGGATTGTTTTATCTAGATCTTCAGAATGAGCTTTATGAGTCTCCTTTTGCTATTTATCATCGAAGGTACAGTACAAACACTAGTCCTAGGTGGCCTCTTGCTCAACCAATGAGGTTTCTTGGACATAACGGGGAGATCAATACCATTCAGGGGAACTTAAATTGGATGCAGTCTCGAGAAGCTTCATTGAAGGCTGCTGTTTGGAATGGCCGTGAAAATGAAATTCGTCCATTTGGTAATCCCAGGGGTTCAGACTCTGCTAATCTTGATAGTGCTGCAGAAATCATGATTAGAAGTGGAAGAACACCAGAGGAAGCTCTAATGATTCTTGTCCCTGAGGCATACAAGAATCATCCAACCTTATCTGTCAAATATCCTGAGGTTGTAGATTTCTATGACTACTACAAAGGACAAATGGAGGCTTGGGATGGTCCTGCCTTACTTTTGTTCAGTGATGGAAAAACAGTTGGGGCTTGTCTTGACCGTAATGGCCTTCGTCCTGCTCGATATTGGCGGACTAGTGACAATTTCGTCTATGTTGCATCTGAGGTCGGAGTTGTACCAGTTGATGAGGCAAAAGTCACAATGAAAGGCCGTCTAGGACCTGGAATGATGATTGCTGTTGACCTTGTGAATGGCCAGGTATATGAGAATACAGAGGTCAAGAAGAGAATATCTTCATTTAATCCATATGGAAAATGGATTAAAGAAAACTCCCGGTTCTTGAAGCCTGTGAATTTCAAATCCTCAACTGTCATGGAAAATGAAGAAATCCTAAGAAGCCAACAAGCATTTGGTTATTCAAGTGAGGATGTGCAAATGGTTATTGAGTCTATGGCTTCTCAAGGAAAGGAACCAACCTTCTGCATGGGCGACGATATTCCGCTGGCAGGATTGTCTCAAAGACCGCATATGCTTTATGATTATTTCAAACAAAGATTTGCACAGGTTACAAACCCTGCCATTGACCCCCTTAGGGAAGGTTTGGTTATGTCTCTTGAAGTAAATATTGGAAAACGTGGAAATATATTGGAGCTTGGACCTGAGAATGCCTCGCAGGTTATTCTGTCTAACCCTGTGTTAAATGAAGGAGCGTTAGAGGAGTTAATGAAGGATCAATACTTAAAACCAAAGGTTCTGTCCACATATTTCGATATAAGAAAAGGCGTTGAAGGTTCCTTGCAAAAGGCTCTATATTATCTTTGTGAAGCAGCTGATGATGCTGTCCGAAGTGGCTCTCAGCTTCTCGTTCTTTCAGACCGATCCGATAGACTGGAACCAACCAGGCCTTCAATTCCAATAATGTTAGCTGTTGGCGCTGTCCATCAACATCTTATTCAGAACGGCTTGCGTATGTCAGCTTCTATTGTTGCTGATACCGCCCAATGCTTCAGCACACATCATTTTGCTTGTTTGGTTGGATATGGTGCAAGTGCTGTATGCCCATACTTGGCACTGGAGACATGTAGGCAATGGCGCTTAAGTAACAAAACTGTGGCCTTCATGCGTAACGGGAAAATTCCTACTGTAACCATTGAGCAAGCTCAGAAGAACTACACTAAGGCGGTTAATGCAGGGCTTCTTAAAATTCTTTCTAAGATGGGAATCTCATTGCTTTCAAGTTATTGTGGTGCTCAGATATTTGAGATATATGGTTTGGGACAGGATGTTGTTGATCTTGCATTCACTGGAAGTGTGTCAAAAATCAGTGGACTCACCTTTGATGAGTTGGCAAGAGAGACATTGTCTTTCTGGGTGAAGGCCTTTTCTGAGGATACAACTAAGCGATTAGAAAATTTTGGGTTTATTCAATTCAGGCCTGGAGGTGAGTATCATTCAAACAACCCAGAGATGTCAAAGTTGCTTCACAAGGCTGTCCGTGAAAAGAGTGAAACTGCATATGCAGTCTATCAACAGCATCTCTCTAACAGACCTGTTAATGTCCTCCGTGACCTGCTTGAGTTCAAGAGTGATCGTGCACCGATCCCAGTAGGGAAAGTAGAACCGGCCGTTGCTATTGTTCAGAGATTTTGTACTGGTGGAATGTCACTTGGTGCTATTTCAAGAGAGACTCATGAAGCTATTGCTATTGCAATGAATAGGATTGGTGGGAAATCAAACTCTGGAGAAGGTGGAGAGGATCCTATCCGTTGGAAGCCACTTACAGATGTGGTTGATGGATATTCACCAACACTACCACATCTCAAAGGTCTTCAAAACGGCGATATTGCAACAAGTGCTATCAAGCAGGTTGCTTCAGGGCGTTTTGGAGTCACACCAACGTTCTTGGTCAATGCAGATCAATTGGAAATCAAAGTTGCACAAGGTGCCAAGCCTGGGGAAGGTGGTCAGCTTCCTGGAAAGAAAGTTAGTGCGTATATCGCTAGGCTAAGAAGCTCTAAACCTGGTGTTCCGCTTATATCTCCGCCTCCTCACCACGACATTTACTCTATTGAGGATCTTGCTCAGTTGATCTTTGATCTACATCAGATTAATCCAAATGCAAAAGTATCAGTCAAGCTAGTTGCAGAAGCTGGAATCGGAACTGTTGCTTCAGGAGTTGCAAAGGGTAACGCTGATATCATCCAGATATCAGGCCATGATGGTGGAACCGGGGCTAGTCCAATAAGCTCCATAAAACATGCTGGTGGACCATGGGAACTTGGACTAACAGAAACTCACCAAACACTTATCGCAAATGGACTAAGAGAAAGAGTCATTTTAAGAGTCGATGGAGGCTTAAAGAGTGGTGTTGATGTTCTAATGGCTGCAGCTATGGGTGCTGATGAATACGGATTCGGTTCCTTGGCAATGATTGCTACTGGTTGTGTTATGGCTCGTATTTGCCACACTAATAATTGCCCAGTGGGTGTAGCAAGTCAGAGAGAAGAATTACGTGCAAGATTCCCTGGTGTACCTGGTGATCTTGTCAACTACTTCTTATACGTAGCAGAAGAGGTGAGAGGTATCTTAGCACAGTTGGGATACAACAGTTTAGATGACATCATTGGACGAACAGAGTTACTGAGACCACGAGACATTTCGCTAGTTAAAACTCAACATCTCGATCTGAGTTATCTTCTTTCGTCTGTTGGAACACCTTCATTGAGCAGTACTGAAATCAGAAAGCAGGAAGTTCATACAAATGGACCTGTTCTCGACGACGATATTCTTGCAGATCCATTGGTGATTGATGCAATAGAGAACGAAAAAGTGGTTGAGAAAACCGTCAAAATATGCAACGTAGACCGTGCGGCTTGTGGTCGTGTTGCTGGTGTTATTGCAAAGAAGTATGGAGACACTGGTTTTGCAGGACAAGTGAACCTAACTTTCTTAGGGAGCGCGGGACAGTCGTTTGGGTGCTTTTTGATTCCCGGTATGAACATCCGGCTCATAGGAGAGTCAAATGACTACGTTGGAAAGGGAATGGCTGGTGGTGAAATAGTAGTAACTCCTGTGGAAAAAATCGGGTTTGTGCCTGAGGAAGCAACGATAGTCGGGAACACTTGCTTGTATGGTGCAACAGGTGGTCAGATATTCGCTAGAGGCAAAGCTGGAGAGAGATTTGCAGTGAGAAACTCACTCGCTGAAGCAGTAGTTGAAGGCACTGGAGACCATTGCTGTGAGTACATGACTGGTGGCTGTGTAGTCGTGCTTGGAAAGGTGGGAAGAAACGTTGCTGCTGGTATGACAGGAGGGTTAGCTTACCTTCTTGATGAAGACGACACTCTTCTTCCTAAGATTAACAGAGAGATAGTGAAGATCCAAAGAGTAACTGCGCCTGCAGGGGAATTGCAGCTGAAGAGCTTAATTGAAGCACATGTGGAAAAAACCGGAAGCAGCAAAGGCGCAACGATTCTGAATGAGTGGGAAAAGTATCTACCTCTCTTCTGGCAACTGGTTCCACCGAGTGAGGAAGACACTCCTGAAGCTTCTGCTGCTTACGTAAGAACATCCACCGGGGAAGTCACATTTCAATCGGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009853 GO:0009941 GO:0009987 GO:0010033 GO:0015930 GO:0016020 GO:0016041 GO:0016053 GO:0016491 GO:0016638 GO:0016643 GO:0019222 GO:0019676 GO:0019740 GO:0019752 GO:0019899 GO:0031323 GO:0031967 GO:0031974 GO:0031975 GO:0034285 GO:0042221 GO:0043085 GO:0043094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0043650 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048046 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051338 GO:0051347 GO:0055114 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0080114 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00630 ko00910 ko01120 map00630 map00910 map01120

Pfam Domains

Protein Families

GATase_2 GXGXG Glu_syn_central Glu_synthase

Protein Analysis

1622

Amino Acids

176.75

Weight (kDa)

5.94

Isoelectric Point (pI)

38.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	106 - 528	6.6e-178	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	555 - 847	2.3e-105	Glutamate synthase central domain
Glu_synthase	PF01645	906 - 1291	3.2e-156	Conserved region in glutamate synthase
GXGXG	PF01493	1372 - 1556	1.7e-77	GXGXG motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005346)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41220 AT5G04140 AT5G04140
fragaria_vesca	FvH4_6g25460 FvH4_6g25460 FvH4_6g25460 FvH4_6g25460
malus_domestica	MD14G1087500.v1.1
prunus_persica	Prupe.7G039100_v2.0.a1
pyrus_communis	pycom14g07760 pycom14g07770
rosa_chinensis	RchiOBHm_Chr2g0125471
rosa_laevigata	RLG00000018438 RLG00000018867 RLG00000030615 RLG00000034746
rosa_multiflora	Rmu_sc0005339.1_g000009 Rmu_sc0005339.1_g000011 Rmu_ssc0000126.1_g000014
rosa_roxburghii	Rroxscaffold_1G00035740 Rroxscaffold_2G00117960
rosa_rugosa	Rorug02G0257500 Rorug02G0257600
rosa_samantha	Rh2AG316500 Rh2BG324000 Rh2BG355400 Rh2CG306200 Rh2DG342600
rosa_wichuraiana	Rw2G025530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1412
Acc36I	ACCTGC	3 cut(s)	1930, 2844, 3121
AccB1I	GGYRCC	1 cut(s)	3209
AccB7I	CCANNNNNTGG	1 cut(s)	468
AccI	GTMKAC	1 cut(s)	4128
AccII	CGCG	1 cut(s)	4226
AciI	CCGC	9 cut(s)	1396, 1766, 1790, 2262, 2447, 3296, 3308, 4136, 4226
AclI	AACGTT	2 cut(s)	3164, 4557
AclWI	GGATC	9 cut(s)	252, 2004, 2139, 2860, 3017, 3030, 3348, 4055, 4639
AcoI	YGGCCR	3 cut(s)	1090, 1519, 2889
AcsI	RAATTY	5 cut(s)	1104, 1618, 2400, 2469, 2677
AcuI	CTGAAG	9 cut(s)	243, 887, 894, 2257, 3123, 3420, 4491, 4703, 4830
AcyI	GRCGYC	1 cut(s)	231
AdeI	CACNNNGTG	3 cut(s)	2936, 3209, 4793
AfaI	GTAC	9 cut(s)	736, 854, 950, 955, 1443, 2917, 3798, 3997, 4511
AfiI	CCNNNNNNNGG	9 cut(s)	359, 468, 665, 1032, 1772, 1858, 2332, 3219, 3224
AflII	CTTAAG	1 cut(s)	2362
AflIII	ACRYGT	3 cut(s)	220, 2345, 4702
AhlI	ACTAGT	2 cut(s)	962, 1400
AjuI	GAANNNNNNNTTGG	4 cut(s)	1875, 1907, 2155, 2187
AloI	GAACNNNNNNTCC	2 cut(s)	609, 641
Alw21I	GWGCWC	2 cut(s)	2518, 2863
Alw44I	GTGCAC	1 cut(s)	2859
AlwI	GGATC	9 cut(s)	252, 2004, 2139, 2860, 3017, 3030, 3348, 4055, 4639
AlwNI	CAGNNNCTG	2 cut(s)	1139, 3416
Ama87I	CYCGRG	1 cut(s)	1056
ApaLI	GTGCAC	1 cut(s)	2859
ApeKI	GCWGC	9 cut(s)	1076, 1157, 2093, 3650, 3653, 4562, 4678, 4722, 4820
ApoI	RAATTY	5 cut(s)	1104, 1618, 2400, 2469, 2677
ArsI	GACNNNNNNTTYG	6 cut(s)	120, 152, 2006, 2038, 4172, 4204
AseI	ATTAAT	1 cut(s)	3375
Asp700I	GAANNNNTTC	4 cut(s)	80, 222, 1558, 3982
AspA2I	CCTAGG	1 cut(s)	968
AspLEI	GCGC	5 cut(s)	2201, 2361, 4226, 4663, 4733
AspS9I	GGNCC	8 cut(s)	123, 489, 786, 1313, 1484, 1922, 3533, 4031
AsuC2I	CCSGG	4 cut(s)	1600, 3496, 4257, 4843
AvaI	CYCGRG	1 cut(s)	1056
AvaII	GGWCC	6 cut(s)	123, 1313, 1484, 1922, 3533, 4031
AvrII	CCTAGG	1 cut(s)	968
AxyI	CCTNAGG	5 cut(s)	273, 1218, 1260, 1856, 4362
BaeGI	GKGCMC	1 cut(s)	2863
BaeI	ACNNNNGTAYC	2 cut(s)	3859, 3892
BalI	TGGCCA	1 cut(s)	1521
BamHI	GGATCC	1 cut(s)	3022
BanI	GGYRCC	1 cut(s)	3209
BarI	GAAGNNNNNNTAC	6 cut(s)	937, 969, 1985, 2017, 2227, 2259
BauI	CACGAG	1 cut(s)	3915
BbsI	GAAGAC	4 cut(s)	450, 3086, 4608, 4806
Bbv12I	GWGCWC	2 cut(s)	2518, 2863
BbvI	GCAGC	9 cut(s)	1063, 1144, 2105, 3637, 3665, 4549, 4690, 4734, 4807
BccI	CCATC	8 cut(s)	434, 1304, 1331, 2214, 2476, 3053, 3479, 3608
BceAI	ACGGC	7 cut(s)	188, 773, 1077, 1461, 2242, 2876, 3118
BcgI	CGANNNNNNTGC	2 cut(s)	4037, 4071
BciVI	GTATCC	2 cut(s)	2650, 3860
BcnI	CCSGG	4 cut(s)	1600, 3496, 4257, 4843
BcuI	ACTAGT	2 cut(s)	962, 1400
BfmI	CTRYAG	4 cut(s)	660, 1158, 3651, 4665
BfoI	RGCGCY	2 cut(s)	2202, 2362
BfrI	CTTAAG	1 cut(s)	2362
BfuAI	ACCTGC	3 cut(s)	1930, 2844, 3121
BfuI	GTATCC	2 cut(s)	2650, 3860
BglII	AGATCT	1 cut(s)	898
BlnI	CCTAGG	1 cut(s)	968
BmcAI	AGTACT	2 cut(s)	736, 3997
Bme18I	GGWCC	6 cut(s)	123, 1313, 1484, 1922, 3533, 4031
BmeT110I	CYCGRG	1 cut(s)	1056
BmgT120I	GGNCC	8 cut(s)	123, 489, 786, 1313, 1484, 1922, 3533, 4031
BmiI	GGNNCC	8 cut(s)	1737, 2059, 2160, 3024, 3211, 3493, 3685, 4785
BmrI	ACTGGG	2 cut(s)	2864, 3740
BmsI	GCATC	6 cut(s)	364, 1038, 1433, 2092, 2809, 4066
BmuI	ACTGGG	2 cut(s)	2864, 3740
BpiI	GAAGAC	4 cut(s)	450, 3086, 4608, 4806
BplI	GAGNNNNNCTC	2 cut(s)	1919, 1951
BpmI	CTGGAG	5 cut(s)	2360, 2724, 3027, 4458, 4512
Bpu10I	CCTNAGC	1 cut(s)	30
BpuEI	CTTGAG	2 cut(s)	1710, 2861
BpuMI	CCSGG	4 cut(s)	1600, 3496, 4257, 4843
BsaAI	YACGTR	3 cut(s)	3779, 3829, 4828
BsaBI	GATNNNNATC	2 cut(s)	2486, 3366
BsaHI	GRCGYC	1 cut(s)	231
BsaI	GGTCTC	2 cut(s)	3904, 4488
BsaWI	WCCGGW	1 cut(s)	4715
Bsc4I	CCNNNNNNNGG	9 cut(s)	359, 468, 665, 1032, 1772, 1858, 2332, 3219, 3224
Bse118I	RCCGGY	1 cut(s)	2887
Bse21I	CCTNAGG	5 cut(s)	273, 1218, 1260, 1856, 4362
Bse3DI	GCAATG	5 cut(s)	2360, 2492, 2982, 3699, 4498
Bse8I	GATNNNNATC	2 cut(s)	2486, 3366
BseGI	GGATG	8 cut(s)	1053, 1234, 1315, 1699, 2554, 3465, 4266, 4835
BseJI	GATNNNNATC	2 cut(s)	2486, 3366
BseLI	CCNNNNNNNGG	9 cut(s)	359, 468, 665, 1032, 1772, 1858, 2332, 3219, 3224
BseMI	GCAATG	5 cut(s)	2360, 2492, 2982, 3699, 4498
BseRI	GAGGAG	4 cut(s)	241, 324, 1997, 3303
BseSI	GKGCMC	1 cut(s)	2863
BseX3I	CGGCCG	1 cut(s)	2889
BseXI	GCAGC	9 cut(s)	1063, 1144, 2105, 3637, 3665, 4549, 4690, 4734, 4807
Bsh1236I	CGCG	1 cut(s)	4226
Bsh1285I	CGRYCG	1 cut(s)	2892
BshNI	GGYRCC	1 cut(s)	3209
BsiEI	CGRYCG	1 cut(s)	2892
BsiHKAI	GWGCWC	2 cut(s)	2518, 2863
BsiHKCI	CYCGRG	1 cut(s)	1056
BsiSI	CCGG	8 cut(s)	769, 1600, 2888, 3495, 4257, 4270, 4716, 4842
BslFI	GGGAC	3 cut(s)	1199, 2556, 4242
BslI	CCNNNNNNNGG	9 cut(s)	359, 468, 665, 1032, 1772, 1858, 2332, 3219, 3224
BsmBI	CGTCTC	1 cut(s)	238
BsmFI	GGGAC	3 cut(s)	1199, 2556, 4242
BsmI	GAATGC	2 cut(s)	1936, 2564
Bso31I	GGTCTC	2 cut(s)	3904, 4488
BsoBI	CYCGRG	1 cut(s)	1056
Bsp1286I	GDGCHC	2 cut(s)	2518, 2863
Bsp1407I	TGTACA	1 cut(s)	852
Bsp19I	CCATGG	1 cut(s)	3536
BspACI	CCGC	9 cut(s)	1396, 1766, 1790, 2262, 2447, 3296, 3308, 4136, 4226
BspFNI	CGCG	1 cut(s)	4226
BspHI	TCATGA	2 cut(s)	1167, 2956
BspLI	GGNNCC	8 cut(s)	1737, 2059, 2160, 3024, 3211, 3493, 3685, 4785
BspMAI	CTGCAG	3 cut(s)	1162, 3655, 4669
BspMI	ACCTGC	3 cut(s)	1930, 2844, 3121
BspPI	GGATC	9 cut(s)	252, 2004, 2139, 2860, 3017, 3030, 3348, 4055, 4639
BspQI	GCTCTTC	2 cut(s)	172, 4679
BspT107I	GGYRCC	1 cut(s)	3209
BspTI	CTTAAG	1 cut(s)	2362
BspTNI	GGTCTC	2 cut(s)	3904, 4488
BsrDI	GCAATG	5 cut(s)	2360, 2492, 2982, 3699, 4498
BsrFI	RCCGGY	1 cut(s)	2887
BsrGI	TGTACA	1 cut(s)	852
BssAI	RCCGGY	1 cut(s)	2887
BssNI	GRCGYC	1 cut(s)	231
BssSI	CACGAG	1 cut(s)	3915
BssT1I	CCWWGG	5 cut(s)	467, 492, 968, 3536, 3687
Bst2BI	CACGAG	1 cut(s)	3915
Bst6I	CTCTTC	8 cut(s)	154, 160, 172, 1544, 3831, 4617, 4679, 4775
BstACI	GRCGYC	1 cut(s)	231
BstAFI	CTTAAG	1 cut(s)	2362
BstAPI	GCANNNNNTGC	3 cut(s)	3119, 3209, 3698
BstAUI	TGTACA	1 cut(s)	852
BstBAI	YACGTR	3 cut(s)	3779, 3829, 4828
BstC8I	GCNNGC	5 cut(s)	863, 1770, 2231, 2424, 4665
BstDSI	CCRYGG	1 cut(s)	3536
BstENI	CCTNNNNNAGG	1 cut(s)	3222
BstF5I	GGATG	8 cut(s)	1053, 1234, 1315, 1699, 2554, 3465, 4266, 4835
BstFNI	CGCG	1 cut(s)	4226
BstH2I	RGCGCY	2 cut(s)	2202, 2362
BstHHI	GCGC	5 cut(s)	2201, 2361, 4226, 4663, 4733
BstMCI	CGRYCG	1 cut(s)	2892
BstNSI	RCATGY	4 cut(s)	865, 2349, 3527, 4706
BstSFI	CTRYAG	4 cut(s)	660, 1158, 3651, 4665
BstSLI	GKGCMC	1 cut(s)	2863
BstSNI	TACGTA	2 cut(s)	3829, 4828
BstUI	CGCG	1 cut(s)	4226
BstV1I	GCAGC	9 cut(s)	1063, 1144, 2105, 3637, 3665, 4549, 4690, 4734, 4807
BstV2I	GAAGAC	4 cut(s)	450, 3086, 4608, 4806
BstX2I	RGATCY	6 cut(s)	244, 898, 3022, 3340, 4060, 4644
BstXI	CCANNNNNNTGG	1 cut(s)	3488
BstYI	RGATCY	6 cut(s)	244, 898, 3022, 3340, 4060, 4644
BstZI	CGGCCG	1 cut(s)	2889
Bsu36I	CCTNAGG	5 cut(s)	273, 1218, 1260, 1856, 4362
BsuI	GTATCC	2 cut(s)	2650, 3860
BtgI	CCRYGG	1 cut(s)	3536
BtgZI	GCGATG	1 cut(s)	20
BtsCI	GGATG	8 cut(s)	1053, 1234, 1315, 1699, 2554, 3465, 4266, 4835
BtsI	GCAGTG	1 cut(s)	4458
BtsIMutI	CAGTG	9 cut(s)	830, 1339, 2336, 2568, 2596, 3753, 4182, 4458, 4488
BveI	ACCTGC	3 cut(s)	1930, 2844, 3121
Cac8I	GCNNGC	5 cut(s)	863, 1770, 2231, 2424, 4665
CaiI	CAGNNNCTG	2 cut(s)	1139, 3416
CciI	TCATGA	2 cut(s)	1167, 2956
CfoI	GCGC	5 cut(s)	2201, 2361, 4226, 4663, 4733
Cfr10I	RCCGGY	1 cut(s)	2887
Cfr13I	GGNCC	8 cut(s)	123, 489, 786, 1313, 1484, 1922, 3533, 4031
CseI	GACGC	2 cut(s)	138, 220
CsiI	ACCWGGT	2 cut(s)	3286, 3799
Csp6I	GTAC	9 cut(s)	735, 853, 949, 954, 1442, 2916, 3797, 3996, 4510
CviQI	GTAC	9 cut(s)	735, 853, 949, 954, 1442, 2916, 3797, 3996, 4510
DraIII	CACNNNGTG	3 cut(s)	2936, 3209, 4793
DrdI	GACNNNNNNGTC	1 cut(s)	1412
DseDI	GACNNNNNNGTC	1 cut(s)	1412
EaeI	YGGCCR	3 cut(s)	1090, 1519, 2889
EagI	CGGCCG	1 cut(s)	2889
Eam1104I	CTCTTC	8 cut(s)	154, 160, 172, 1544, 3831, 4617, 4679, 4775
EarI	CTCTTC	8 cut(s)	154, 160, 172, 1544, 3831, 4617, 4679, 4775
EciI	GGCGGA	2 cut(s)	1411, 3297
EclXI	CGGCCG	1 cut(s)	2889
Eco105I	TACGTA	2 cut(s)	3829, 4828
Eco130I	CCWWGG	5 cut(s)	467, 492, 968, 3536, 3687
Eco147I	AGGCCT	3 cut(s)	2170, 2645, 2701
Eco31I	GGTCTC	2 cut(s)	3904, 4488
Eco32I	GATATC	2 cut(s)	3463, 3474
Eco47I	GGWCC	6 cut(s)	123, 1313, 1484, 1922, 3533, 4031
Eco52I	CGGCCG	1 cut(s)	2889
Eco57I	CTGAAG	9 cut(s)	243, 887, 894, 2257, 3123, 3420, 4491, 4703, 4830
Eco81I	CCTNAGG	5 cut(s)	273, 1218, 1260, 1856, 4362
Eco88I	CYCGRG	1 cut(s)	1056
EcoNI	CCTNNNNNAGG	1 cut(s)	3222
EcoO109I	RGGNCCY	1 cut(s)	1484
EcoRV	GATATC	2 cut(s)	3463, 3474
EcoT14I	CCWWGG	5 cut(s)	467, 492, 968, 3536, 3687
ErhI	CCWWGG	5 cut(s)	467, 492, 968, 3536, 3687
Esp3I	CGTCTC	1 cut(s)	238
FalI	AAGNNNNNCTT	6 cut(s)	65, 97, 298, 330, 363, 395
FaqI	GGGAC	3 cut(s)	1199, 2556, 4242
FauI	CCCGC	1 cut(s)	4219
FauNDI	CATATG	3 cut(s)	1573, 1794, 2782
FblI	GTMKAC	1 cut(s)	4128
FokI	GGATG	8 cut(s)	1060, 1221, 1322, 1706, 2561, 3452, 4253, 4822
GlaI	GCGC	5 cut(s)	2200, 2360, 4225, 4662, 4732
GsuI	CTGGAG	5 cut(s)	2360, 2724, 3027, 4458, 4512
HaeII	RGCGCY	2 cut(s)	2202, 2362
HapII	CCGG	8 cut(s)	769, 1600, 2888, 3495, 4257, 4270, 4716, 4842
HgaI	GACGC	2 cut(s)	138, 220
HhaI	GCGC	5 cut(s)	2201, 2361, 4226, 4663, 4733
Hin1I	GRCGYC	1 cut(s)	231
Hin6I	GCGC	5 cut(s)	2199, 2359, 4224, 4661, 4731
HinP1I	GCGC	5 cut(s)	2199, 2359, 4224, 4661, 4731
HincII	GTYRAC	2 cut(s)	1507, 3814
HindII	GTYRAC	2 cut(s)	1507, 3814
HindIII	AAGCTT	3 cut(s)	32, 1062, 4812
HpaII	CCGG	8 cut(s)	769, 1600, 2888, 3495, 4257, 4270, 4716, 4842
Hpy99I	CGWCG	4 cut(s)	92, 1760, 4046, 4049
Hsp92I	GRCGYC	1 cut(s)	231
HspAI	GCGC	5 cut(s)	2199, 2359, 4224, 4661, 4731
LguI	GCTCTTC	2 cut(s)	172, 4679
LmnI	GCTCC	7 cut(s)	311, 419, 514, 1915, 1973, 3518, 4221
Lsp1109I	GCAGC	9 cut(s)	1063, 1144, 2105, 3637, 3665, 4549, 4690, 4734, 4807
LweI	GCATC	6 cut(s)	364, 1038, 1433, 2092, 2809, 4066
MabI	ACCWGGT	2 cut(s)	3286, 3799
MfeI	CAATTG	1 cut(s)	3185
MflI	RGATCY	6 cut(s)	244, 898, 3022, 3340, 4060, 4644
MhlI	GDGCHC	2 cut(s)	2518, 2863
MlsI	TGGCCA	1 cut(s)	1521
MluNI	TGGCCA	1 cut(s)	1521
MmeI	TCCRAC	7 cut(s)	79, 1262, 1414, 2281, 3014, 3955, 4279
Mox20I	TGGCCA	1 cut(s)	1521
MroXI	GAANNNNTTC	4 cut(s)	80, 222, 1558, 3982
MscI	TGGCCA	1 cut(s)	1521
MslI	CAYNNNNRTG	5 cut(s)	531, 3486, 3528, 4026, 4503
Msp20I	TGGCCA	1 cut(s)	1521
MspA1I	CMGCKG	3 cut(s)	1768, 2096, 4681
MspCI	CTTAAG	1 cut(s)	2362
MspI	CCGG	8 cut(s)	769, 1600, 2888, 3495, 4257, 4270, 4716, 4842
MunI	CAATTG	1 cut(s)	3185
Mva1269I	GAATGC	2 cut(s)	1936, 2564
MvnI	CGCG	1 cut(s)	4226
NciI	CCSGG	4 cut(s)	1600, 3496, 4257, 4843
NcoI	CCATGG	1 cut(s)	3536
NdeI	CATATG	3 cut(s)	1573, 1794, 2782
NlaIV	GGNNCC	8 cut(s)	1737, 2059, 2160, 3024, 3211, 3493, 3685, 4785
NmuCI	GTSAC	6 cut(s)	1403, 1462, 2831, 2928, 3154, 4849
NspI	RCATGY	4 cut(s)	865, 2349, 3527, 4706
PaeI	GCATGC	1 cut(s)	865
PaeR7I	CTCGAG	1 cut(s)	1056
PagI	TCATGA	2 cut(s)	1167, 2956
PasI	CCCWGGG	1 cut(s)	1125
PceI	AGGCCT	3 cut(s)	2170, 2645, 2701
PciI	ACATGT	2 cut(s)	2345, 4702
PciSI	GCTCTTC	2 cut(s)	172, 4679
PcsI	WCGNNNNNNNCGW	4 cut(s)	93, 136, 786, 1384
PctI	GAATGC	2 cut(s)	1936, 2564
PdmI	GAANNNNTTC	4 cut(s)	80, 222, 1558, 3982
PfeI	GAWTC	9 cut(s)	431, 1207, 1231, 2487, 3420, 3678, 3786, 4252, 4738
PflFI	GACNNNGTC	1 cut(s)	2626
PflMI	CCANNNNNTGG	1 cut(s)	468
PfoI	TCCNGGA	1 cut(s)	3238
Ppu21I	YACGTR	3 cut(s)	3779, 3829, 4828
PpuMI	RGGWCCY	1 cut(s)	1484
PscI	ACATGT	2 cut(s)	2345, 4702
PshBI	ATTAAT	1 cut(s)	3375
Psp1406I	AACGTT	2 cut(s)	3164, 4557
Psp5II	RGGWCCY	1 cut(s)	1484
PspN4I	GGNNCC	8 cut(s)	1737, 2059, 2160, 3024, 3211, 3493, 3685, 4785
PspPI	GGNCC	8 cut(s)	123, 489, 786, 1313, 1484, 1922, 3533, 4031
PspPPI	RGGWCCY	1 cut(s)	1484
PstI	CTGCAG	3 cut(s)	1162, 3655, 4669
PstNI	CAGNNNCTG	2 cut(s)	1139, 3416
PsuI	RGATCY	6 cut(s)	244, 898, 3022, 3340, 4060, 4644
PsyI	GACNNNGTC	1 cut(s)	2626
PvuII	CAGCTG	2 cut(s)	2096, 4681
RsaI	GTAC	9 cut(s)	736, 854, 950, 955, 1443, 2917, 3798, 3997, 4511
RsaNI	GTAC	9 cut(s)	735, 853, 949, 954, 1442, 2916, 3797, 3996, 4510
RseI	CAYNNNNRTG	5 cut(s)	531, 3486, 3528, 4026, 4503
SapI	GCTCTTC	2 cut(s)	172, 4679
Sau96I	GGNCC	8 cut(s)	123, 489, 786, 1313, 1484, 1922, 3533, 4031
SbfI	CCTGCAGG	1 cut(s)	4669
ScaI	AGTACT	2 cut(s)	736, 3997
SdaI	CCTGCAGG	1 cut(s)	4669
SduI	GDGCHC	2 cut(s)	2518, 2863
SexAI	ACCWGGT	2 cut(s)	3286, 3799
SfaNI	GCATC	6 cut(s)	364, 1038, 1433, 2092, 2809, 4066
SfcI	CTRYAG	4 cut(s)	660, 1158, 3651, 4665
Sfr274I	CTCGAG	1 cut(s)	1056
SinI	GGWCC	6 cut(s)	123, 1313, 1484, 1922, 3533, 4031
SlaI	CTCGAG	1 cut(s)	1056
SmiMI	CAYNNNNRTG	5 cut(s)	531, 3486, 3528, 4026, 4503
SmlI	CTYRAG	4 cut(s)	1056, 1725, 2362, 2840
SmoI	CTYRAG	4 cut(s)	1056, 1725, 2362, 2840
SnaBI	TACGTA	2 cut(s)	3829, 4828
SpeI	ACTAGT	2 cut(s)	962, 1400
SphI	GCATGC	1 cut(s)	865
Sse8387I	CCTGCAGG	1 cut(s)	4669
SseBI	AGGCCT	3 cut(s)	2170, 2645, 2701
SsiI	CCGC	9 cut(s)	1396, 1766, 1790, 2262, 2447, 3296, 3308, 4136, 4226
SspI	AATATT	1 cut(s)	1891
StuI	AGGCCT	3 cut(s)	2170, 2645, 2701
StyI	CCWWGG	5 cut(s)	467, 492, 968, 3536, 3687
TaqII	GACCGA	1 cut(s)	2157
TatI	WGTACW	6 cut(s)	734, 852, 953, 2915, 3995, 4509
TauI	GCSGC	1 cut(s)	4139
TfiI	GAWTC	9 cut(s)	431, 1207, 1231, 2487, 3420, 3678, 3786, 4252, 4738
TscAI	CASTG	9 cut(s)	837, 1339, 2343, 2575, 2596, 3753, 4189, 4458, 4495
TseFI	GTSAC	6 cut(s)	1403, 1462, 2831, 2928, 3154, 4849
TseI	GCWGC	9 cut(s)	1076, 1157, 2093, 3650, 3653, 4562, 4678, 4722, 4820
Tsp45I	GTSAC	6 cut(s)	1403, 1462, 2831, 2928, 3154, 4849
TspGWI	ACGGA	4 cut(s)	2750, 2819, 3020, 3690
TspRI	CASTG	9 cut(s)	837, 1339, 2343, 2575, 2596, 3753, 4189, 4458, 4495
Tth111I	GACNNNGTC	1 cut(s)	2626
Van91I	CCANNNNNTGG	1 cut(s)	468
Vha464I	CTTAAG	1 cut(s)	2362
VneI	GTGCAC	1 cut(s)	2859
VpaK11BI	GGWCC	6 cut(s)	123, 1313, 1484, 1922, 3533, 4031
VspI	ATTAAT	1 cut(s)	3375
XagI	CCTNNNNNAGG	1 cut(s)	3222
XapI	RAATTY	5 cut(s)	1104, 1618, 2400, 2469, 2677
XbaI	TCTAGA	1 cut(s)	895
XceI	RCATGY	4 cut(s)	865, 2349, 3527, 4706
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1578, 1673
XhoI	CTCGAG	1 cut(s)	1056
XmaJI	CCTAGG	1 cut(s)	968
XmiI	GTMKAC	1 cut(s)	4128
XmnI	GAANNNNTTC	4 cut(s)	80, 222, 1558, 3982
ZrmI	AGTACT	2 cut(s)	736, 3997

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.