Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0005346

Physical Location & Seq

Genomic Coordinates Reverse (-)

44748163 .. 44773971

Gene Structure 25809 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG342600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4473 bp

ATGGAACATCGTGGAGGCTGTGGAGCAGATAATGACTCTGGTGATGGTTCAGGATTGATGAGCTCAATCCCATGGGATCTATTTAACAATTGGGCTGACAAGCAAGGGATTGCATCATTTGACAAGTTACACACTGGTGTTGGAATGATTTTCCTCCCCAAAGAGGACAACCTTATGAAAGAAGCCAAAAAAGCTATTGTGAATATTTTTAAACAAGAGGGTCTTGAAGTGCTTGGGTGGAGACCTGTTCCAGTGAATACAGCTATAGTTGGTTTCTATGCAAAAGAAACAATGCCCAGCATACAACAGGTATTCGTCAAAGTTGTTAAAGAGGAAAAAGTTGAGGACATTGAGCGAGAACTGTACATATGCCGGAAGTTAATCGAAAGAGCAGCAAGCTCAGAAAGTTGGGGCAGTGATCTTTATTTCTGTTCTTTGTCCAATCAAACAATAGTTTACAAAGGAATGCTACGCTCAGAAGTTCTTGGTTTATTTTATTCTGACCTCCAAAGTGACCTTTATAAATCCGCTTTTGCCATCTATCATCGAAGGTATAGCACAAATACCACTCCTAGATGGCCCCTTGCACAACCAATGAGGTTACTTGGTCACAATGGAGAGATCAACACCATACAGGGGAACTTGAACTGGATGCAATCACGTGAAGCCTCACTGAAGTCACCTGTATGGCATGATCGTGAAAATGAACTTCGTCCATATGGCAATCCAAAGGGTTCAGACTCTGCGAATCTTGACAGTGCGGCTGAATTCTTACTAAGAAGTGGCCGGACTGCTGAGGAGGCTCTGATGATTCTTGTCCCTGAGGGATACAAAAATCATCCAACTCTGATGATTAACTATCCAGAGGTGGTTGATTTTTATGACTATTACAAGGGTCAGATGGAGGCTTGGGATGGACCTGCTTTATTGTTATTCAGTGATGGAAAAACAGTTGGGGCATGTCTTGATCGTAATGGACTTCGGCCTGCTAGATATTGGCGGACATCTGATAATGTTGTTTATGTCGCATCTGAGGTTGGGGTTCTTCCAGTGGATGATTCAAAAGTTACAATGAAAGGTCGTCTAGGTCCTGGAATGATGATATCTGTTGACTTACTAAGTGGTCAGGTTTATGAGAACACAGAAGTTAAGAAGCGAGTGGCTTTGTCAAATCCATATGGAAAGTGGGTGCAGGAAAACATGCGGACCTTAAAGACTGTGAACTTCCTTTCATCCACAATCGCGGACAATGATGCAATACTGAGACGACAACAGGCATTTGGTTACTCGAGTGAGGATGTCCAAATGGTTATCGAGACAATGGCTTCACAAGGGAAGGAGCCTACATTTTGCATGGGCGATGATATTCCATTGGCGATTTTATCTCAGAGACCACATATGCTATATGACTATTTCAAGCAACGGTTTGCGCAGGTTACAAATCCAGCCATTGATCCTCTAAGAGAAGGGTTAGTTATGTCTCTTGAAGTCAACATTGGAAAGCGACAAAATATTTTGGAGGTTGGACCTGAAAATGCATCGCAGGTTATCTTGTCCAGTCCTGTGCTCAATGAAGGAGAACTTGAGTCATTGCTTAAGGATGCTCAGCTAAAACCCCAAGTTTTACCTACATTTTTTGATATTCATAAAGGGGTCGATGGTTCCTTGGAAAAAACGCTGTATAAGCTTTGTGAAGCTGCTGATGATGCTGTTCAAAATGGTTGCCAATTGCTTGTTCTGTCTGACCGGTCTGATGAATTGGAAGCAACTCGTCCTGCCATTCCAATCCTTCTTGCTGTTGGTGCTGTTCATCAGCATCTTATTCAAAATGGGTTGAGAATGTCAGCTTCTATAATAGTGGATACTGCTCAGTGCTTTAGCACTCATCAATTTGCCTGTTTGATTGGATATGGTGCAAGTGCTGTGTGTCCATACTTGGCATTGGAGACATGTCGGCAGTGGCGTCTGAGTAATAAAACAGTAAATCTAATGCGGAACGGGAAGATGCCTACTGTAACGATTGAACAGGCTCAAAAGAACTTCTGCAAGGCTGTTACGGCTGGTCTTCTTAAAATTCTTTCCAAAATGGGGATCTCGTTGCTTTCAAGTTATTGCGGTGCACAGATTTTTGAGATTTATGGATTGGGGAAGGAGGTTGTTGATCTTGCATTTTGTGGGAGTATATCAAGTATTGGTGGATTAACTTTTGATGAGCTGGCGAGGGAGACTTTGTCCTTCTGGGTGAAGGCTTTCTCTGAGGATACAGCTAAACGACTGGAAAATTTTGGATTTATACAATTCAGACCAGGAGGGGAGTATCATGGAAACAACCCAGAGATGTCGAAATTGCTACACAAAGCTGTTCGACAGAAGAATGAAAGTGCCTTTTCTGTTTATCAGCAGCATTTAGCTAACCGGCCCGTTAATGTTCTTCGCGATCTCATTGAGTTCAAAAGCGATCGTTCTCCAATCCCTGTGGGAAAGGTTGAACCTGCTGTATCCATTGTTCAACGATTCTGCACTGGTGGGATGTCACTTGGAGCTATTTCCAGAGAAACTCATGAGGCTATAGCCATTGCAATGAATAGAATAGGTGGAAAGTCCAATTCTGGAGAAGGTGGTGAGGATCCTATTCGCTGGAAACCACTTGCAGATGTTGTTGATGGGTATTCACCGACCCTTCCACATCTAAAAGGTCTTCAAAATGGAGATACTGCTACAAGTGCTATCAAGCAGGTTGCTTCAGGACGTTTTGGAGTCACTCCCACCTTCTTGGTCAATGCCGATCAGCTGGAAATCAAAATTGCACAAGGGGCGAAGCCTGGTGAAGGTGGCCAACTGCCTGGTAAAAAAGTTAGTGCATACATTGCGAGGTTGAGAAACTCGAAACCTGGGGTTCCACTTATATCTCCACCTCCACACCATGACATTTATTCTATAGAGGATCTTGCCCAATTGATCTATGACCTTCATCAGGTGAACCCTAAAGCTAAGGTGTCAGTGAAACTTGTGGCTGAAGCTGGAATTGGCACTGTTGCTTCTGGGGTTGCAAAGGGTAATGCTGATATCATACAGATATCAGGGCATGATGGTGGAACTGGGGCCAGTCCTATAAGTTCCATTAAGCATGCTGGTGGGCCCTGGGAACTAGGACTCACAGAAACACACCAGACACTCATAGAAAATGGACTTAGAGAACGAGTCATTCTGAGAGTTGATGGTGGCTTTAAAAGCGGAGTTGATGTCCTAATGGCTGCAACTATGGGTGCTGATGAGTACGGGTTTGGGTCTGTGGCAATGATTGCTACAGGTTGTGTTATGGCCCGCATTTGCCATACGAATAATTGTCCTGTTGGTGTAGCCAGCCAGAGAGAGGAACTGCGTGCTCGATTTCCTGGCGTGCCTGGTGATCTAGTCAACTTTTTTTTGTATGTTGCCGAGGAGGTAAGAGGCATGTTGGCACAGTTGGGATATGAGAAGTTAGACGATATAATTGGACGGACAGATTTGTTTAGACCACGAGATATATCTCTAATGAAAACACAGCATCTTGATCTCGGTTATATTCTATCAAATGTCGGGTTACCAAAATGGAGCAGTACCATGATCAGGAATCAGGATGTTCATACTAACGGTCCTGTTCTGGATGATATTTTACTAGGAGATCCAGAGATATCAGATGCAATTGAGAATGAAAAAGTGGTTCAGAAGACCGTAAAGATATACAATGTGGATCGTGCTGTTTGTGGGCGTATAGCAGGGGTGGTTGCCAAAAAGTACGGTGACACTGGTTTTGCTGGGCAGCTGAACATAACCTTTACAGGGAGCGCTGGGCAATCATTTGGTTGCTTCTTGACTCCTGGGATGAATATTCGACTTGTTGGAGAGGCAAATGATTATGTGGGAAAGAGTATTTCGGGGGGAGAATTGGTTGTCACTCCGGCAGAGAATACGGGATTTTGCCCTGAGGATGCAACTATTGTAGGGAATACCTGCTTGTATGGTGCAACTGGGGGTCAAATCTTTGTTAGAGGGAAAGCCGGAGAGCGATTTGCTGTAAGAAACTCTCTTGCACAAGCAGTGGTTGAAGGCACTGGAGACCACTGTTGTGAATACATGACTGGTGGCTGTGTAGTTGTACTTGGAAAAGTGGGTAGAAATGTTGCTGCTGGAATGACCGGAGGTCTGGCATACTTTCTTGATGAGGACGATTCTTTCATCCCCAAGGTAAATAGGGAGATTGTGAAGATCCAGAGAGTCAATGCTCCTGTGGGGCAGATGCAGCTAAAGACCCTTATTGAAGCCCATGTTGAAAAAACTGGAAGTGGCAAGGGCTCTGTTATTCTGAAAGAGTGGGACAAGTACTTGCCACTCTTCTGGCAGCTGGTTCCCCCTAGTGAAGAAGACACTCCAGAGGCGTGTGCAGACTATGTGAGAACAGCTGCTGGTGAAGTGACTTTGCAGTCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009853 GO:0009941 GO:0009987 GO:0010033 GO:0015930 GO:0016020 GO:0016041 GO:0016053 GO:0016491 GO:0016638 GO:0016643 GO:0019222 GO:0019676 GO:0019740 GO:0019752 GO:0019899 GO:0031323 GO:0031967 GO:0031974 GO:0031975 GO:0034285 GO:0042221 GO:0043085 GO:0043094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0043650 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048046 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051338 GO:0051347 GO:0055114 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0080114 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00630 ko00910 ko01120 map00630 map00910 map01120

Pfam Domains

Protein Families

GATase_2 GXGXG Glu_syn_central Glu_synthase

Protein Analysis

1490

Amino Acids

162.63

Weight (kDa)

5.86

Isoelectric Point (pI)

34.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	1 - 396	3.2e-164	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	422 - 715	1.5e-107	Glutamate synthase central domain
Glu_synthase	PF01645	775 - 1158	2.2e-155	Conserved region in glutamate synthase
GXGXG	PF01493	1240 - 1423	1.6e-78	GXGXG motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005346)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41220 AT5G04140 AT5G04140
fragaria_vesca	FvH4_6g25460 FvH4_6g25460 FvH4_6g25460 FvH4_6g25460
malus_domestica	MD14G1087500.v1.1
prunus_persica	Prupe.7G039100_v2.0.a1
pyrus_communis	pycom14g07760 pycom14g07770
rosa_chinensis	RchiOBHm_Chr2g0125471
rosa_laevigata	RLG00000018438 RLG00000018867 RLG00000030615 RLG00000034746
rosa_multiflora	Rmu_sc0005339.1_g000009 Rmu_sc0005339.1_g000011 Rmu_ssc0000126.1_g000014
rosa_roxburghii	Rroxscaffold_1G00035740 Rroxscaffold_2G00117960
rosa_rugosa	Rorug02G0257500 Rorug02G0257600
rosa_samantha	Rh2AG316500 Rh2BG324000 Rh2BG355400 Rh2CG306200 Rh2DG342600
rosa_wichuraiana	Rw2G025530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	522
AasI	GACNNNNNNGTC	1 cut(s)	4462
Acc16I	TGCGCA	1 cut(s)	1431
Acc36I	ACCTGC	6 cut(s)	928, 1423, 1534, 2500, 2725, 4001
AccII	CGCG	2 cut(s)	1244, 2436
AciI	CCGC	9 cut(s)	528, 761, 1000, 1204, 1244, 1993, 2115, 3234, 3325
AclWI	GGATC	9 cut(s)	84, 1448, 2099, 2621, 2634, 2952, 3659, 3741, 4243
AcoI	YGGCCR	2 cut(s)	784, 2833
AcsI	RAATTY	3 cut(s)	767, 2073, 2281
AcuI	CTGAAG	3 cut(s)	695, 2727, 3036
AcvI	CACGTG	1 cut(s)	662
AcyI	GRCGYC	1 cut(s)	1963
AfaI	GTAC	6 cut(s)	365, 3278, 3601, 3779, 4140, 4364
AfeI	AGCGCT	1 cut(s)	3829
AfiI	CCNNNNNNNGG	8 cut(s)	163, 636, 2088, 2828, 2974, 3373, 3609, 3822
AflII	CTTAAG	1 cut(s)	1595
AflIII	ACRYGT	1 cut(s)	1949
AgeI	ACCGGT	1 cut(s)	1746
AhdI	GACNNNNNGTC	1 cut(s)	4182
AjnI	CCWGG	9 cut(s)	1090, 2305, 2821, 2842, 2890, 3140, 3394, 3403, 3859
AjuI	GAANNNNNNNTTGG	6 cut(s)	1479, 1511, 1719, 1751, 3009, 3041
AleI	CACNNNNGTG	2 cut(s)	135, 4107
Alw21I	GWGCWC	4 cut(s)	65, 1569, 2122, 3388
Alw26I	GTCTC	8 cut(s)	235, 1258, 1310, 1384, 1485, 1940, 2219, 4092
Alw44I	GTGCAC	1 cut(s)	2118
AlwI	GGATC	9 cut(s)	84, 1448, 2099, 2621, 2634, 2952, 3659, 3741, 4243
AlwNI	CAGNNNCTG	2 cut(s)	743, 4445
Ama87I	CYCGRG	1 cut(s)	1288
Aor51HI	AGCGCT	1 cut(s)	3829
AoxI	GGCC	8 cut(s)	578, 784, 983, 2417, 2833, 3102, 3137, 3321
ApaI	GGGCCC	1 cut(s)	3141
ApaLI	GTGCAC	1 cut(s)	2118
ApeKI	GCWGC	9 cut(s)	392, 1697, 2401, 3254, 3802, 4166, 4282, 4381, 4442
ApoI	RAATTY	3 cut(s)	767, 2073, 2281
ArsI	GACNNNNNNTTYG	2 cut(s)	3776, 3808
AsiGI	ACCGGT	1 cut(s)	1746
Asp700I	GAANNNNTTC	1 cut(s)	2249
AspLEI	GCGC	2 cut(s)	1432, 3830
AvaI	CYCGRG	1 cut(s)	1288
AvaII	GGWCC	5 cut(s)	917, 1088, 1206, 1526, 3635
AxyI	CCTNAGG	2 cut(s)	822, 3966
BaeGI	GKGCMC	2 cut(s)	2122, 3141
BalI	TGGCCA	1 cut(s)	2835
BamHI	GGATCC	1 cut(s)	2626
BanII	GRGCYC	3 cut(s)	65, 3141, 4337
BauI	CACGAG	1 cut(s)	3519
BbrPI	CACGTG	1 cut(s)	662
BbsI	GAAGAC	4 cut(s)	2057, 2690, 3716, 4410
Bbv12I	GWGCWC	4 cut(s)	65, 1569, 2122, 3388
BbvCI	CCTCAGC	1 cut(s)	795
BbvI	GCAGC	9 cut(s)	404, 1684, 2413, 3241, 3814, 4153, 4294, 4393, 4429
BceAI	ACGGC	1 cut(s)	2073
BciT130I	CCWGG	9 cut(s)	1092, 2307, 2823, 2844, 2892, 3142, 3396, 3405, 3861
BciVI	GTATCC	4 cut(s)	821, 1855, 2254, 2509
BclI	TGATCA	1 cut(s)	3606
BcoDI	GTCTC	8 cut(s)	235, 1258, 1310, 1384, 1485, 1940, 2219, 4092
BfaI	CTAG	7 cut(s)	573, 990, 1085, 3149, 3413, 3659, 4395
BfmI	CTRYAG	4 cut(s)	264, 2568, 2937, 3306
BfoI	RGCGCY	1 cut(s)	3831
BfrI	CTTAAG	1 cut(s)	1595
BfuAI	ACCTGC	6 cut(s)	928, 1423, 1534, 2500, 2725, 4001
BfuI	GTATCC	4 cut(s)	821, 1855, 2254, 2509
BlpI	GCTNAGC	1 cut(s)	1605
BmcAI	AGTACT	1 cut(s)	4364
Bme1390I	CCNGG	9 cut(s)	1092, 2307, 2823, 2844, 2892, 3142, 3396, 3405, 3861
Bme18I	GGWCC	5 cut(s)	917, 1088, 1206, 1526, 3635
BmeRI	GACNNNNNGTC	1 cut(s)	4182
BmeT110I	CYCGRG	1 cut(s)	1288
BmiI	GGNNCC	8 cut(s)	581, 1341, 1663, 2628, 2898, 3103, 3139, 4389
BmrFI	CCNGG	9 cut(s)	1092, 2307, 2823, 2844, 2892, 3142, 3396, 3405, 3861
BmrI	ACTGGG	2 cut(s)	3108, 4020
BmuI	ACTGGG	2 cut(s)	3108, 4020
BpiI	GAAGAC	4 cut(s)	2057, 2690, 3716, 4410
BpmI	CTGGAG	3 cut(s)	2631, 4116, 4395
Bpu10I	CCTNAGC	2 cut(s)	795, 2991
Bpu1102I	GCTNAGC	1 cut(s)	1605
BpuEI	CTTGAG	1 cut(s)	1604
BsaAI	YACGTR	1 cut(s)	662
BsaHI	GRCGYC	1 cut(s)	1963
BsaI	GGTCTC	3 cut(s)	235, 1384, 4092
BsaJI	CCNNGG	8 cut(s)	71, 1665, 2891, 3140, 3141, 3438, 3860, 4224
BsaWI	WCCGGW	2 cut(s)	1746, 4178
BsaXI	ACNNNNNCTCC	6 cut(s)	2216, 2246, 3228, 3258, 3915, 3945
Bsc4I	CCNNNNNNNGG	8 cut(s)	163, 636, 2088, 2828, 2974, 3373, 3609, 3822
Bse118I	RCCGGY	2 cut(s)	1746, 2415
Bse21I	CCTNAGG	2 cut(s)	822, 3966
Bse3DI	GCAATG	5 cut(s)	1589, 2574, 2586, 2865, 3303
BseBI	CCWGG	9 cut(s)	1092, 2307, 2823, 2844, 2892, 3142, 3396, 3405, 3861
BseDI	CCNNGG	8 cut(s)	71, 1665, 2891, 3140, 3141, 3438, 3860, 4224
BseLI	CCNNNNNNNGG	8 cut(s)	163, 636, 2088, 2828, 2974, 3373, 3609, 3822
BseMI	GCAATG	5 cut(s)	1589, 2574, 2586, 2865, 3303
BseRI	GAGGAG	2 cut(s)	812, 3455
BseSI	GKGCMC	2 cut(s)	2122, 3141
BseXI	GCAGC	9 cut(s)	404, 1684, 2413, 3241, 3814, 4153, 4294, 4393, 4429
BseYI	CCCAGC	3 cut(s)	296, 3797, 3830
BsgI	GTGCAG	3 cut(s)	1211, 2503, 4443
Bsh1236I	CGCG	2 cut(s)	1244, 2436
Bsh1285I	CGRYCG	1 cut(s)	2461
BshFI	GGCC	8 cut(s)	580, 786, 985, 2419, 2835, 3104, 3139, 3323
BshTI	ACCGGT	1 cut(s)	1746
BsiEI	CGRYCG	1 cut(s)	2461
BsiHKAI	GWGCWC	4 cut(s)	65, 1569, 2122, 3388
BsiHKCI	CYCGRG	1 cut(s)	1288
BsiSI	CCGG	7 cut(s)	373, 787, 1747, 2416, 3941, 4041, 4179
BslFI	GGGAC	2 cut(s)	803, 4370
BslI	CCNNNNNNNGG	8 cut(s)	163, 636, 2088, 2828, 2974, 3373, 3609, 3822
BsmAI	GTCTC	8 cut(s)	235, 1258, 1310, 1384, 1485, 1940, 2219, 4092
BsmBI	CGTCTC	1 cut(s)	1258
BsmFI	GGGAC	2 cut(s)	803, 4370
BsmI	GAATGC	1 cut(s)	471
BsnI	GGCC	8 cut(s)	580, 786, 985, 2419, 2835, 3104, 3139, 3323
Bso31I	GGTCTC	3 cut(s)	235, 1384, 4092
BsoBI	CYCGRG	1 cut(s)	1288
Bsp120I	GGGCCC	1 cut(s)	3137
Bsp1286I	GDGCHC	6 cut(s)	65, 1569, 2122, 3141, 3388, 4337
Bsp1407I	TGTACA	1 cut(s)	363
Bsp1720I	GCTNAGC	1 cut(s)	1605
Bsp19I	CCATGG	1 cut(s)	71
Bsp68I	TCGCGA	1 cut(s)	2436
BspACI	CCGC	9 cut(s)	528, 761, 1000, 1204, 1244, 1993, 2115, 3234, 3325
BspANI	GGCC	8 cut(s)	580, 786, 985, 2419, 2835, 3104, 3139, 3323
BspFNI	CGCG	2 cut(s)	1244, 2436
BspHI	TCATGA	1 cut(s)	2560
BspLI	GGNNCC	8 cut(s)	581, 1341, 1663, 2628, 2898, 3103, 3139, 4389
BspMI	ACCTGC	6 cut(s)	928, 1423, 1534, 2500, 2725, 4001
BspPI	GGATC	9 cut(s)	84, 1448, 2099, 2621, 2634, 2952, 3659, 3741, 4243
BspTI	CTTAAG	1 cut(s)	1595
BspTNI	GGTCTC	3 cut(s)	235, 1384, 4092
BsrDI	GCAATG	5 cut(s)	1589, 2574, 2586, 2865, 3303
BsrFI	RCCGGY	2 cut(s)	1746, 2415
BsrGI	TGTACA	1 cut(s)	363
BssAI	RCCGGY	2 cut(s)	1746, 2415
BssECI	CCNNGG	8 cut(s)	71, 1665, 2891, 3140, 3141, 3438, 3860, 4224
BssNI	GRCGYC	1 cut(s)	1963
BssSI	CACGAG	1 cut(s)	3519
BssT1I	CCWWGG	3 cut(s)	71, 1665, 4224
Bst2BI	CACGAG	1 cut(s)	3519
Bst2UI	CCWGG	9 cut(s)	1092, 2307, 2823, 2844, 2892, 3142, 3396, 3405, 3861
Bst6I	CTCTTC	1 cut(s)	4379
BstACI	GRCGYC	1 cut(s)	1963
BstAFI	CTTAAG	1 cut(s)	1595
BstAPI	GCANNNNNTGC	2 cut(s)	2867, 3302
BstAUI	TGTACA	1 cut(s)	363
BstBAI	YACGTR	1 cut(s)	662
BstC8I	GCNNGC	8 cut(s)	397, 987, 2217, 3129, 3325, 3364, 3384, 3401
BstDSI	CCRYGG	1 cut(s)	71
BstEII	GGTNACC	1 cut(s)	3582
BstENI	CCTNNNNNAGG	2 cut(s)	2826, 2972
BstFNI	CGCG	2 cut(s)	1244, 2436
BstH2I	RGCGCY	1 cut(s)	3831
BstHHI	GCGC	2 cut(s)	1432, 3830
BstMAI	GTCTC	8 cut(s)	235, 1258, 1310, 1384, 1485, 1940, 2219, 4092
BstMCI	CGRYCG	1 cut(s)	2461
BstNI	CCWGG	9 cut(s)	1092, 2307, 2823, 2844, 2892, 3142, 3396, 3405, 3861
BstNSI	RCATGY	5 cut(s)	963, 1204, 1953, 3131, 3457
BstPI	GGTNACC	1 cut(s)	3582
BstSCI	CCNGG	9 cut(s)	1090, 2305, 2821, 2842, 2890, 3140, 3394, 3403, 3859
BstSFI	CTRYAG	4 cut(s)	264, 2568, 2937, 3306
BstSLI	GKGCMC	2 cut(s)	2122, 3141
BstUI	CGCG	2 cut(s)	1244, 2436
BstV1I	GCAGC	9 cut(s)	404, 1684, 2413, 3241, 3814, 4153, 4294, 4393, 4429
BstV2I	GAAGAC	4 cut(s)	2057, 2690, 3716, 4410
BstX2I	RGATCY	6 cut(s)	76, 2091, 2626, 2944, 3664, 4248
BstXI	CCANNNNNNTGG	3 cut(s)	2773, 2843, 4377
BstYI	RGATCY	6 cut(s)	76, 2091, 2626, 2944, 3664, 4248
Bsu36I	CCTNAGG	2 cut(s)	822, 3966
BsuI	GTATCC	4 cut(s)	821, 1855, 2254, 2509
BsuRI	GGCC	8 cut(s)	580, 786, 985, 2419, 2835, 3104, 3139, 3323
BtgI	CCRYGG	1 cut(s)	71
BtgZI	GCGATG	2 cut(s)	1374, 1524
BtsI	GCAGTG	3 cut(s)	421, 1964, 4086
BtuMI	TCGCGA	1 cut(s)	2436
BveI	ACCTGC	6 cut(s)	928, 1423, 1534, 2500, 2725, 4001
Cac8I	GCNNGC	8 cut(s)	397, 987, 2217, 3129, 3325, 3364, 3384, 3401
CaiI	CAGNNNCTG	2 cut(s)	743, 4445
CciI	TCATGA	1 cut(s)	2560
CfoI	GCGC	2 cut(s)	1432, 3830
Cfr10I	RCCGGY	2 cut(s)	1746, 2415
CseI	GACGC	1 cut(s)	1952
Csp6I	GTAC	6 cut(s)	364, 3277, 3600, 3778, 4139, 4363
CspAI	ACCGGT	1 cut(s)	1746
CspCI	CAANNNNNGTGG	2 cut(s)	2457, 2492
CviQI	GTAC	6 cut(s)	364, 3277, 3600, 3778, 4139, 4363
DraI	TTTAAA	2 cut(s)	211, 3229
DrdI	GACNNNNNNGTC	1 cut(s)	4462
DriI	GACNNNNNGTC	1 cut(s)	4182
DseDI	GACNNNNNNGTC	1 cut(s)	4462
EaeI	YGGCCR	2 cut(s)	784, 2833
Eam1104I	CTCTTC	1 cut(s)	4379
Eam1105I	GACNNNNNGTC	1 cut(s)	4182
EarI	CTCTTC	1 cut(s)	4379
EciI	GGCGGA	1 cut(s)	1015
Ecl136II	GAGCTC	1 cut(s)	63
Eco130I	CCWWGG	3 cut(s)	71, 1665, 4224
Eco24I	GRGCYC	3 cut(s)	65, 3141, 4337
Eco31I	GGTCTC	3 cut(s)	235, 1384, 4092
Eco32I	GATATC	4 cut(s)	1104, 3067, 3078, 3675
Eco47I	GGWCC	5 cut(s)	917, 1088, 1206, 1526, 3635
Eco47III	AGCGCT	1 cut(s)	3829
Eco53kI	GAGCTC	1 cut(s)	63
Eco57I	CTGAAG	3 cut(s)	695, 2727, 3036
Eco72I	CACGTG	1 cut(s)	662
Eco81I	CCTNAGG	2 cut(s)	822, 3966
Eco88I	CYCGRG	1 cut(s)	1288
Eco91I	GGTNACC	1 cut(s)	3582
EcoICRI	GAGCTC	1 cut(s)	63
EcoNI	CCTNNNNNAGG	2 cut(s)	2826, 2972
EcoO109I	RGGNCCY	2 cut(s)	1088, 3138
EcoO65I	GGTNACC	1 cut(s)	3582
EcoRI	GAATTC	1 cut(s)	767
EcoRII	CCWGG	9 cut(s)	1090, 2305, 2821, 2842, 2890, 3140, 3394, 3403, 3859
EcoRV	GATATC	4 cut(s)	1104, 3067, 3078, 3675
EcoT14I	CCWWGG	3 cut(s)	71, 1665, 4224
EcoT22I	ATGCAT	1 cut(s)	1540
EcoT38I	GRGCYC	3 cut(s)	65, 3141, 4337
ErhI	CCWWGG	3 cut(s)	71, 1665, 4224
Esp3I	CGTCTC	1 cut(s)	1258
FalI	AAGNNNNNCTT	4 cut(s)	207, 239, 1566, 1598
FaqI	GGGAC	2 cut(s)	803, 4370
FauI	CCCGC	1 cut(s)	3332
FauNDI	CATATG	4 cut(s)	368, 718, 1177, 1398
FbaI	TGATCA	1 cut(s)	3606
FriOI	GRGCYC	3 cut(s)	65, 3141, 4337
FspBI	CTAG	7 cut(s)	573, 990, 1085, 3149, 3413, 3659, 4395
FspI	TGCGCA	1 cut(s)	1431
GlaI	GCGC	2 cut(s)	1431, 3829
GsaI	CCCAGC	3 cut(s)	300, 3801, 3834
GsuI	CTGGAG	3 cut(s)	2631, 4116, 4395
HaeII	RGCGCY	1 cut(s)	3831
HaeIII	GGCC	8 cut(s)	580, 786, 985, 2419, 2835, 3104, 3139, 3323
HapII	CCGG	7 cut(s)	373, 787, 1747, 2416, 3941, 4041, 4179
HgaI	GACGC	1 cut(s)	1952
HhaI	GCGC	2 cut(s)	1432, 3830
Hin1I	GRCGYC	1 cut(s)	1963
Hin6I	GCGC	2 cut(s)	1430, 3828
HinP1I	GCGC	2 cut(s)	1430, 3828
HincII	GTYRAC	3 cut(s)	1111, 1492, 3418
HindII	GTYRAC	3 cut(s)	1111, 1492, 3418
HindIII	AAGCTT	1 cut(s)	1685
HpaII	CCGG	7 cut(s)	373, 787, 1747, 2416, 3941, 4041, 4179
Hpy166II	GTNNAC	7 cut(s)	457, 1111, 1222, 1492, 2120, 2980, 3418
Hpy8I	GTNNAC	7 cut(s)	457, 1111, 1222, 1492, 2120, 2980, 3418
HpyCH4IV	ACGT	2 cut(s)	661, 2749
HpySE526I	ACGT	2 cut(s)	661, 2749
Hsp92I	GRCGYC	1 cut(s)	1963
HspAI	GCGC	2 cut(s)	1430, 3828
Ksp22I	TGATCA	1 cut(s)	3606
LmnI	GCTCC	6 cut(s)	23, 1339, 2540, 3594, 3825, 4270
Lsp1109I	GCAGC	9 cut(s)	404, 1684, 2413, 3241, 3814, 4153, 4294, 4393, 4429
MaeI	CTAG	7 cut(s)	573, 990, 1085, 3149, 3413, 3659, 4395
MaeII	ACGT	2 cut(s)	661, 2749
MfeI	CAATTG	4 cut(s)	88, 1727, 2954, 3684
MflI	RGATCY	6 cut(s)	76, 2091, 2626, 2944, 3664, 4248
MhlI	GDGCHC	6 cut(s)	65, 1569, 2122, 3141, 3388, 4337
MlsI	TGGCCA	1 cut(s)	2835
MluNI	TGGCCA	1 cut(s)	2835
MlyI	GAGTC	8 cut(s)	29, 734, 1595, 2766, 3147, 3210, 3850, 4266
MmeI	TCCRAC	4 cut(s)	121, 866, 1504, 3862
Mox20I	TGGCCA	1 cut(s)	2835
Mph1103I	ATGCAT	1 cut(s)	1540
MroXI	GAANNNNTTC	1 cut(s)	2249
MscI	TGGCCA	1 cut(s)	2835
MslI	CAYNNNNRTG	7 cut(s)	135, 685, 696, 2579, 3090, 3132, 4107
Msp20I	TGGCCA	1 cut(s)	2835
MspA1I	CMGCKG	4 cut(s)	2791, 3805, 4384, 4442
MspCI	CTTAAG	1 cut(s)	1595
MspI	CCGG	7 cut(s)	373, 787, 1747, 2416, 3941, 4041, 4179
MspR9I	CCNGG	9 cut(s)	1092, 2307, 2823, 2844, 2892, 3142, 3396, 3405, 3861
MunI	CAATTG	4 cut(s)	88, 1727, 2954, 3684
Mva1269I	GAATGC	1 cut(s)	471
MvaI	CCWGG	9 cut(s)	1092, 2307, 2823, 2844, 2892, 3142, 3396, 3405, 3861
MvnI	CGCG	2 cut(s)	1244, 2436
NcoI	CCATGG	1 cut(s)	71
NdeI	CATATG	4 cut(s)	368, 718, 1177, 1398
NlaIV	GGNNCC	8 cut(s)	581, 1341, 1663, 2628, 2898, 3103, 3139, 4389
NmeAIII	GCCGAG	1 cut(s)	3463
NmuCI	GTSAC	8 cut(s)	512, 608, 678, 2532, 2758, 3782, 3934, 4453
NruI	TCGCGA	1 cut(s)	2436
NsbI	TGCGCA	1 cut(s)	1431
NsiI	ATGCAT	1 cut(s)	1540
NspI	RCATGY	5 cut(s)	963, 1204, 1953, 3131, 3457
OliI	CACNNNNGTG	2 cut(s)	135, 4107
PaeI	GCATGC	1 cut(s)	3131
PaeR7I	CTCGAG	1 cut(s)	1288
PagI	TCATGA	1 cut(s)	2560
PasI	CCCWGGG	1 cut(s)	3141
PciI	ACATGT	1 cut(s)	1949
PcsI	WCGNNNNNNNCGW	1 cut(s)	1960
PctI	GAATGC	1 cut(s)	471
PdmI	GAANNNNTTC	1 cut(s)	2249
PfeI	GAWTC	6 cut(s)	748, 811, 1058, 2514, 3613, 4211
PflFI	GACNNNGTC	1 cut(s)	2230
PfoI	TCCNGGA	1 cut(s)	1090
PinAI	ACCGGT	1 cut(s)	1746
Ple19I	CGATCG	1 cut(s)	2461
PleI	GAGTC	8 cut(s)	29, 734, 1594, 2765, 3147, 3209, 3850, 4265
PmaCI	CACGTG	1 cut(s)	662
PmlI	CACGTG	1 cut(s)	662
PpsI	GAGTC	8 cut(s)	29, 734, 1594, 2765, 3147, 3209, 3850, 4265
Ppu21I	YACGTR	1 cut(s)	662
PpuMI	RGGWCCY	1 cut(s)	1088
PscI	ACATGT	1 cut(s)	1949
PsiI	TTATAA	1 cut(s)	522
Psp124BI	GAGCTC	1 cut(s)	65
Psp5II	RGGWCCY	1 cut(s)	1088
Psp6I	CCWGG	9 cut(s)	1090, 2305, 2821, 2842, 2890, 3140, 3394, 3403, 3859
PspCI	CACGTG	1 cut(s)	662
PspEI	GGTNACC	1 cut(s)	3582
PspFI	CCCAGC	3 cut(s)	296, 3797, 3830
PspGI	CCWGG	9 cut(s)	1090, 2305, 2821, 2842, 2890, 3140, 3394, 3403, 3859
PspN4I	GGNNCC	8 cut(s)	581, 1341, 1663, 2628, 2898, 3103, 3139, 4389
PspOMI	GGGCCC	1 cut(s)	3137
PspPPI	RGGWCCY	1 cut(s)	1088
PspXI	VCTCGAGB	1 cut(s)	1288
PsrI	GAACNNNNNNTAC	2 cut(s)	2490, 2522
PstNI	CAGNNNCTG	2 cut(s)	743, 4445
PsuI	RGATCY	6 cut(s)	76, 2091, 2626, 2944, 3664, 4248
PsyI	GACNNNGTC	1 cut(s)	2230
PvuI	CGATCG	1 cut(s)	2461
PvuII	CAGCTG	4 cut(s)	2791, 3805, 4384, 4442
RruI	TCGCGA	1 cut(s)	2436
RsaI	GTAC	6 cut(s)	365, 3278, 3601, 3779, 4140, 4364
RsaNI	GTAC	6 cut(s)	364, 3277, 3600, 3778, 4139, 4363
RseI	CAYNNNNRTG	7 cut(s)	135, 685, 696, 2579, 3090, 3132, 4107
SacI	GAGCTC	1 cut(s)	65
ScaI	AGTACT	1 cut(s)	4364
SchI	GAGTC	8 cut(s)	29, 734, 1595, 2766, 3147, 3210, 3850, 4266
ScrFI	CCNGG	9 cut(s)	1092, 2307, 2823, 2844, 2892, 3142, 3396, 3405, 3861
SduI	GDGCHC	6 cut(s)	65, 1569, 2122, 3141, 3388, 4337
SfcI	CTRYAG	4 cut(s)	264, 2568, 2937, 3306
Sfr274I	CTCGAG	1 cut(s)	1288
SinI	GGWCC	5 cut(s)	917, 1088, 1206, 1526, 3635
SlaI	CTCGAG	1 cut(s)	1288
SmiMI	CAYNNNNRTG	7 cut(s)	135, 685, 696, 2579, 3090, 3132, 4107
SmlI	CTYRAG	3 cut(s)	1288, 1583, 1595
SmoI	CTYRAG	3 cut(s)	1288, 1583, 1595
SphI	GCATGC	1 cut(s)	3131
SsiI	CCGC	9 cut(s)	528, 761, 1000, 1204, 1244, 1993, 2115, 3234, 3325
SspI	AATATT	3 cut(s)	205, 1513, 3871
SspMI	CTAG	7 cut(s)	573, 990, 1085, 3149, 3413, 3659, 4395
SstI	GAGCTC	1 cut(s)	65
StyD4I	CCNGG	9 cut(s)	1090, 2305, 2821, 2842, 2890, 3140, 3394, 3403, 3859
StyI	CCWWGG	3 cut(s)	71, 1665, 4224
TaiI	ACGT	2 cut(s)	664, 2752
TatI	WGTACW	3 cut(s)	363, 4138, 4362
TauI	GCSGC	1 cut(s)	764
TfiI	GAWTC	6 cut(s)	748, 811, 1058, 2514, 3613, 4211
TseFI	GTSAC	8 cut(s)	512, 608, 678, 2532, 2758, 3782, 3934, 4453
TseI	GCWGC	9 cut(s)	392, 1697, 2401, 3254, 3802, 4166, 4282, 4381, 4442
Tsp45I	GTSAC	8 cut(s)	512, 608, 678, 2532, 2758, 3782, 3934, 4453
TspGWI	ACGGA	1 cut(s)	3514
Tth111I	GACNNNGTC	1 cut(s)	2230
Vha464I	CTTAAG	1 cut(s)	1595
VneI	GTGCAC	1 cut(s)	2118
VpaK11BI	GGWCC	5 cut(s)	917, 1088, 1206, 1526, 3635
XagI	CCTNNNNNAGG	2 cut(s)	2826, 2972
XapI	RAATTY	3 cut(s)	767, 2073, 2281
XceI	RCATGY	5 cut(s)	963, 1204, 1953, 3131, 3457
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1182
XhoI	CTCGAG	1 cut(s)	1288
XmnI	GAANNNNTTC	1 cut(s)	2249
XspI	CTAG	7 cut(s)	573, 990, 1085, 3149, 3413, 3659, 4395
ZrmI	AGTACT	1 cut(s)	4364
Zsp2I	ATGCAT	1 cut(s)	1540

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.