Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005346

Physical Location & Seq

Genomic Coordinates Reverse (-)

10899805 .. 10927540

Gene Structure 27736 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1087500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4887 bp

ATGGCTCTGCAGTCGGTGACGCCGATTGCTCAGCTTCTTCACTGCTCCAGCAATGGCCGCTCCCCCGCCACCCAGCCGCCCCGGAACGGACTCTTCGTCGTCGATTTCGTCGGCTTGTGCGGTAAATCGAAGCGGACTCGCAGGAAATTCGGAGCCGCCTCCGACCATCGCATCTTCCCTCATTTCGTCTCCAGGAACTGCCACTCGGTCAACGCCGTTCTTGACGTCGGACGCAGCAGCGACGCCGCTGCCTCGGATCAATCAGCTCCGATGACTCCTGATTTAAAACCTAAGGTTGCAGACTTGCAAGATATAATATCAGAAAGAGGAGCTTGTGGAGTTGGATTTATTGCCAATTTGGAAAACAAAGCATCCCATGGCATTGTTGAAGATGCTCTAACAGCCCTTGGCTGCATGGAACATCGTGGGGGCTGTGGAGCAGATAATGACTCTGGTGATGGTTCGGGATTGATGAGTTCAATCCCCTGGGATCTATTTGACAATTGGGCTAACAAGCAAGGAATTGCTTCCTTTGACAAGTTGCACACCGGTGTTGGAATGGTTTTCCTACCACAAGATGACAACCTTATGAAAGAAGCCAAAAATGTTGTTGTGAATATTTTTAGACAAGAGGGTCTTGAAGTGCTTGGATGGAGACCTGTTCCCGTGAATACTTCTTTAGTTGGTTACTATGCAAAAGAAACAATGCCAAGCATACAACAGGTATTTGTTAAAGTTGTTAAAGAGGAAAATGTTGAGGACATTGAAAGGGAACTGTACATATGCCGAAAGTTGATCGAGAGAGCAGCAAGCTCAGAAAGTTGGGGAAATGAGCTTTATTTCTGTTCTTTGTCCAATCAAACAATAGTTTACAAGGGAATGCTCCGGTCAGAAGTTCTTGGTTTATTTTATTCTGACCTCCAAAGTGACCTGTATAAATCCCCTTTTGCCATCTATCATCGGAGGTATAGCACAAATACCACTCCCAGGTGGCCCCTTGCACAACCAATGAGGTTACTTGGTCACAATGGAGAGATTAATACCATACAGGGAAACTTGAACTGGATGCAATCTCGTGAAGCCTCATTGAAATCACCTGTATGGAATGGTCGTGAAAATGAAATTCGCCCATTTGGCAATCCGAAGGCATCTGACTCTGCAAATCTTGACAGTGCAGCCGAATTCTTATTAAGAAGTGGCCGCAGTGCTGAGGAGGCTCTAATGATTCTTGTCCCAGAGGGATACAAAAATCACCCGACTTTGTCGGTAAAATATCCTGAGGTGATTGATTTTTATGACTATTACAAGGGTCAGATGGAGGCTTGGGATGGACCTGCTTTACTGTTATTCAGTGATGGAAAAACAGTTGGGGCCTGTCTTGACCGGAATGGTCTTCGCCCGGCTAGGTATTGGAGGACATCTGATAATGTTGTATATGTTGCATCTGAGGTTGGTGTTGTACCTGTGGATGATTCTAAAATTACAATGAAAGGTCGTCTAGGTCCTGGAATGATGATAGCTGCTGATTTAATAAGTGGTCAGGTTTATGAGAACACGGAAGTTAAAAAGCGTGTAGCTTTGTCAAATCCATATGGAAAGTGGCTACAGGAAAACATGCGATCGTTGAAGGCTGCGAACTTCCTTTCAGCCACAGTTGCAGAAAATGATGCAATACTAAGACGGCAACAGGCATTTGGCTACTCCAGTGAGGATGTACAAATGGTTATTGAGACTATGGCTTCACAAGGGAAGGAGCCAACATTTTGTATGGGTGACGATATTCCATTGGCAATATTATCTCAGAGGCCACATATGCTTTATGACTATTTCAAGCAACGGTTTGCACAGGTTACAAATCCAGCCATTGATCCTCTTCGAGAAGGTTTAGTTATGTCTCTTGAAATCAACATTGGAAAGCGACAAAATATTTTGGAGGTTGGACCTGAAAATGCATCGCAGGTTATCTTGTCTAGTCCTGTTCTGAATGAAGGAGAACTTGATTTGTTGCTAAAGGATGCCAAGTTGAAACCCCAAGTTTTACCTACATTTTTTGATATCCATAAAGGGGTTGATGGTTCCTTGGAAAAGACTCTATATAGGCTTTGTGAAGCTGCTGATGAAGCTGTTCAAAATGGTTGCCAACTGCTTGTGCTTTCTGATCGGGCTGATGAGCTGGAGGCAACTCGACCTGCAATTCCCATCCTTCTTGCTGTTGGTGCTGTTCATCAGCATCTTATTCAAAATGGCTTGAGAATGTCAGCTTCTATCATAGTAGACACTGCTCAGTGTTTTAGCACTCACCAGTTTGCCTGCTTGATTGGATATGGTGCAAGTGCTATTTGTCCATACTTGGCATTAGAGACGTGTCGGCAATGGCGTCTGAGTGCTAAAACTGTAAATTTAATGCGTAATGGGAAGATGCCTACTGTAACAATTGAACAGGCTCAAAAGAATTTCTGCAAGGCTGTTAAAGCTGGTCTTCTTAAAATTCTTTCCAAAATGGGCATCTCGTTGCTTTCAAGTTATTGTGGTGCACAGATATTTGAGATCTATGGATTGGGGAAGGAGGTTGTTGATCTTGCATTTTGTGGAAGTATATCTTGTATTGGTGGATTAACTTTTGATGAGCTTGCAAGGGAGACTTTGTCCTTCTGGGTGAAGGCTTTCTCTGAGGATACAGCTAAAAGACTTGAAAATTTTGGATTTATACAATTCAGGCCAGGAGGGGAGTATCATGGAAACAACCCAGAGATGTCAAAATTGCTCCACAAAGCTGTCCGCCAAAAAAATGAAAGTGCTTTTTCAGTTTATCAACAGCATTTAGCTAACCGACCTGTAAATGTTCTTCGTGATCTTGTTGAGTTCAAAAGTGATCGTACTCCAATTCCTGTTGGAAAAGTTGAACCTGCTGTATCCATTGTTCAACGGTTCTGCACTGGTGGGATGTCACTTGGAGCTATTTCAAGAGAAACTCACGAGGCTATTGCCATTGCAATGAATAGAATAGGTGGAAAGTCCAATTCTGGAGAAGGTGGTGAGGATCCTATTCGTTGGAAACCACTTACAGATGTCGTTGATGGATACTCTCCCACCCTTCCACATCTTAAGGGTCTTCAAAATGGAGATACTGCTACAAGTGCTATCAAGCAGGTTGCTTCAGGACGTTTTGGTGTCACTCCAACCTTCTTGGTCAATGCTGATCAGCTGGAAATTAAAATTGCACAAGGTGCGAAGCCCGGTGAAGGTGGCCAATTGCCTGGTAAAAAAGTCAGTGCATATATTGCGAGGTTGAGAAATTCTAAACCAGGGGTTCCACTTATATCTCCACCTCCACACCATGACATTTATTCCATAGAGGATCTTGCCCAATTGATCTATGATCTTCATCAGGTGAACCCTAAAGCTAAGGTGTCAGTAAAGCTTGTGGCAGAAGCTGGAATTGGCACTGTTGCTTCTGGTGTTGCAAAGGGTAATGCTGATGTCATTCAGATATCAGGTCATGATGGTGGAACTGGTGCCAGTCCTATAAGTTCCATCAAGCATGCTGGTGGTCCCTGGGAACTGGGACTTACAGAAACACACCAGACACTCATTTCAAATGGACTTAGAGAACGAGTTATTTTAAGAGTTGATGGCGGCTTTAAAAGTGGAGTCGATGTCTTAATGGCTGCAGCAATGGGTGCTGATGAATATGGATTTGGTTCTGTTGCAATGATTGCTACAGGGTGTGTTATGGCCCGCATTTGCCATACAAATAATTGTCCAGTTGGTGTAGCCAGTCAGAGAGAAGAACTGCGTGCTCGATTTCCTGGAGTCCCTGGTGATCTTGTCAATTTTTTTCTATATGTTGCTGAGGAGGTAAGAGGTATGTTAGCACAGTTGGGATATGAGAAGTTAGACGATATAATTGGGCAGACAAATTTACTGAGGCCACGAGATATATCTCTAGTGAAAACTCAGCATCTTGATCTCAGTTATCTTCTCTCAAATGTTGGGTTACCAAAATGGAGCAGTACCATAATCAGGAACCAAGATGTTCACACTAATGGACCTGTTCTGGATGATGTTTTACTAGCAGATCAAGAGATATCCGATGCAATTGAGAATGAAAAGGTGGTCCACAAGACCATAAAGATATACAATGTTGACCGTGCAGTTTGTGGACGTATAGCAGGCGTGGTTGCCAAAAAATATGGTGACACTGGTTTTGCTGGGCAGCTAAACATAACTTTTCAAGGGAGTGCTGGGCAGTCATTTGGGTGCTTCTTGACTCCTGGGATGAACATTCAACTTGTTGGAGAGGCAAATGATTATGTCGGGAAGAGTATTTCCGGCGGAGAATTGGTTGTGACTCCAGTTGAGAATACTGGGTTTTGCCCGGAGGACGCAACAATTGTAGGGAATACCTGCTTGTATGGAGCCACTGGGGGTCAAATCTTCATTAGAGGGAAAGCTGGGGAACGATTTGCTGTCAGAAACTCACTTGCGCAAGCAGTGGTTGAAGGAACTGGAGACCACTGTCTTGAATATATGACTGGCGGTTGTGTGGTGGTACTTGGAAAAGTCGGTAGAAATGTTGCTGCTGGAATGACTGGAGGTTTGGCATACATCCTTGATGAGGACGATACTTTCATCCCCAAGGTAAATAGGGAGATCGTCAAAATCCAAAGAGTGAATGCTCCTGTGGGGCAGATGCAGCTAAAGAGCCTTATCAAAGCCCATGTTGAAAAAACTGGAAGCGGCAAGGGCGCAGCTATCCTGAAGGAGTGGGACAAATATTTACCACTCTTTTGGCAGCTGGTTCCACCTAGTGAAGAAGACACCCCAGAGGCATGTGCAGATTATGAGCAAACAGCTGCAGGTGCGGTGACTTTGCAGTCCGCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009853 GO:0009941 GO:0009987 GO:0010033 GO:0015930 GO:0016020 GO:0016041 GO:0016053 GO:0016491 GO:0016638 GO:0016643 GO:0019222 GO:0019676 GO:0019740 GO:0019752 GO:0019899 GO:0031323 GO:0031967 GO:0031974 GO:0031975 GO:0034285 GO:0042221 GO:0043085 GO:0043094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0043650 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048046 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051338 GO:0051347 GO:0055114 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0080114 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00630 ko00910 ko01120 map00630 map00910 map01120

Pfam Domains

Protein Families

GATase_2 GXGXG Glu_syn_central Glu_synthase

Protein Analysis

1629

Amino Acids

176.92

Weight (kDa)

6.16

Isoelectric Point (pI)

35.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	112 - 534	1.9e-176	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	560 - 853	6.3e-107	Glutamate synthase central domain
Glu_synthase	PF01645	913 - 1296	3.1e-155	Conserved region in glutamate synthase
GXGXG	PF01493	1378 - 1561	3.9e-77	GXGXG motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005346)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41220 AT5G04140 AT5G04140
fragaria_vesca	FvH4_6g25460 FvH4_6g25460 FvH4_6g25460 FvH4_6g25460
malus_domestica	MD14G1087500.v1.1
prunus_persica	Prupe.7G039100_v2.0.a1
pyrus_communis	pycom14g07760 pycom14g07770
rosa_chinensis	RchiOBHm_Chr2g0125471
rosa_laevigata	RLG00000018438 RLG00000018867 RLG00000030615 RLG00000034746
rosa_multiflora	Rmu_sc0005339.1_g000009 Rmu_sc0005339.1_g000011 Rmu_ssc0000126.1_g000014
rosa_roxburghii	Rroxscaffold_1G00035740 Rroxscaffold_2G00117960
rosa_rugosa	Rorug02G0257500 Rorug02G0257600
rosa_samantha	Rh2AG316500 Rh2BG324000 Rh2BG355400 Rh2CG306200 Rh2DG342600
rosa_wichuraiana	Rw2G025530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	4850
AasI	GACNNNNNNGTC	2 cut(s)	633, 4876
AatII	GACGTC	1 cut(s)	228
Acc16I	TGCGCA	1 cut(s)	4488
Acc36I	ACCTGC	7 cut(s)	1342, 1948, 2197, 2914, 3139, 4415, 4850
AccB1I	GGYRCC	1 cut(s)	3515
AccBSI	CCGCTC	1 cut(s)	60
AccI	GTMKAC	1 cut(s)	2274
AccII	CGCG	1 cut(s)	4883
AclWI	GGATC	6 cut(s)	264, 498, 1862, 3035, 3048, 3366
AcoI	YGGCCR	3 cut(s)	55, 1198, 3247
AcsI	RAATTY	9 cut(s)	146, 1122, 1181, 2398, 2452, 2487, 2695, 3295, 3919
AcuI	CTGAAG	2 cut(s)	3141, 4781
AcyI	GRCGYC	4 cut(s)	20, 225, 243, 2377
AdeI	CACNNNGTG	2 cut(s)	3227, 4811
AfaI	GTAC	6 cut(s)	779, 1461, 1716, 2878, 4015, 4554
AfiI	CCNNNNNNNGG	6 cut(s)	86, 987, 2065, 3242, 4023, 4618
AflII	CTTAAG	1 cut(s)	3104
AflIII	ACRYGT	1 cut(s)	2363
AgeI	ACCGGT	1 cut(s)	548
AhdI	GACNNNNNGTC	1 cut(s)	95
AjiI	CACGTC	1 cut(s)	2364
AjuI	GAANNNNNNNTTGG	6 cut(s)	1893, 1925, 3423, 3455, 3681, 3713
AleI	CACNNNNGTG	1 cut(s)	549
Alw21I	GWGCWC	2 cut(s)	2536, 3802
Alw26I	GTCTC	7 cut(s)	193, 649, 1724, 1899, 2354, 2633, 4506
Alw44I	GTGCAC	1 cut(s)	2532
AlwI	GGATC	6 cut(s)	264, 498, 1862, 3035, 3048, 3366
AlwNI	CAGNNNCTG	2 cut(s)	198, 3434
AoxI	GGCC	9 cut(s)	55, 992, 1198, 1371, 1805, 2717, 3247, 3735, 3929
ApaLI	GTGCAC	1 cut(s)	2532
ApoI	RAATTY	9 cut(s)	146, 1122, 1181, 2398, 2452, 2487, 2695, 3295, 3919
ArsI	GACNNNNNNTTYG	4 cut(s)	4190, 4222, 4582, 4614
AseI	ATTAAT	1 cut(s)	1038
AsiGI	ACCGGT	1 cut(s)	548
Asp700I	GAANNNNTTC	3 cut(s)	526, 2124, 2663
AspLEI	GCGC	2 cut(s)	4489, 4751
AspS9I	GGNCC	9 cut(s)	993, 1331, 1371, 1502, 1940, 3551, 3736, 4049, 4117
AsuC2I	CCSGG	4 cut(s)	82, 1400, 3237, 4379
AvaII	GGWCC	6 cut(s)	1331, 1502, 1940, 3551, 4049, 4117
AxyI	CCTNAGG	2 cut(s)	291, 1278
BaeGI	GKGCMC	1 cut(s)	2536
BalI	TGGCCA	1 cut(s)	3249
BamHI	GGATCC	1 cut(s)	3040
BanI	GGYRCC	1 cut(s)	3515
BarI	GAAGNNNNNNTAC	2 cut(s)	2829, 2861
BauI	CACGAG	3 cut(s)	1074, 2975, 3933
BbsI	GAAGAC	4 cut(s)	1385, 2471, 3104, 4824
Bbv12I	GWGCWC	2 cut(s)	2536, 3802
BbvCI	CCTCAGC	2 cut(s)	1209, 3852
BceAI	ACGGC	2 cut(s)	200, 1697
BcgI	CGANNNNNNTGC	6 cut(s)	130, 164, 230, 264, 2966, 3000
BciVI	GTATCC	4 cut(s)	1235, 2668, 2923, 3074
BclI	TGATCA	1 cut(s)	3199
BcnI	CCSGG	4 cut(s)	82, 1400, 3237, 4379
BcoDI	GTCTC	7 cut(s)	193, 649, 1724, 1899, 2354, 2633, 4506
BfaI	CTAG	6 cut(s)	1404, 1499, 1971, 3947, 4073, 4809
BfmI	CTRYAG	5 cut(s)	8, 1604, 3669, 3720, 4857
BfrI	CTTAAG	1 cut(s)	3104
BfuAI	ACCTGC	7 cut(s)	1342, 1948, 2197, 2914, 3139, 4415, 4850
BfuI	GTATCC	4 cut(s)	1235, 2668, 2923, 3074
BglI	GCCNNNNNGGC	1 cut(s)	1134
BglII	AGATCT	1 cut(s)	2547
BlpI	GCTNAGC	1 cut(s)	30
Bme18I	GGWCC	6 cut(s)	1331, 1502, 1940, 3551, 4049, 4117
BmeRI	GACNNNNNGTC	1 cut(s)	95
BmgBI	CACGTC	1 cut(s)	2364
BmgT120I	GGNCC	9 cut(s)	993, 1331, 1371, 1502, 1940, 3551, 3736, 4049, 4117
BmrI	ACTGGG	3 cut(s)	3572, 4377, 4434
BmuI	ACTGGG	3 cut(s)	3572, 4377, 4434
BoxI	GACNNNNGTC	1 cut(s)	4518
BpiI	GAAGAC	4 cut(s)	1385, 2471, 3104, 4824
BpmI	CTGGAG	9 cut(s)	31, 175, 1687, 2195, 3045, 3831, 4338, 4530, 4614
Bpu10I	CCTNAGC	3 cut(s)	1209, 3405, 3852
Bpu1102I	GCTNAGC	1 cut(s)	30
BpuEI	CTTGAG	1 cut(s)	2269
BpuMI	CCSGG	4 cut(s)	82, 1400, 3237, 4379
BsaBI	GATNNNNATC	1 cut(s)	3384
BsaHI	GRCGYC	4 cut(s)	20, 225, 243, 2377
BsaI	GGTCTC	2 cut(s)	649, 4506
BsaWI	WCCGGW	3 cut(s)	548, 885, 1383
BsaXI	ACNNNNNCTCC	6 cut(s)	2630, 2660, 3642, 3672, 4329, 4359
Bsc4I	CCNNNNNNNGG	6 cut(s)	86, 987, 2065, 3242, 4023, 4618
Bse118I	RCCGGY	1 cut(s)	548
Bse21I	CCTNAGG	2 cut(s)	291, 1278
Bse3DI	GCAATG	6 cut(s)	58, 2378, 2988, 3000, 3681, 3717
Bse8I	GATNNNNATC	1 cut(s)	3384
BseJI	GATNNNNATC	1 cut(s)	3384
BseLI	CCNNNNNNNGG	6 cut(s)	86, 987, 2065, 3242, 4023, 4618
BseMI	GCAATG	6 cut(s)	58, 2378, 2988, 3000, 3681, 3717
BseRI	GAGGAG	3 cut(s)	342, 1226, 3869
BseSI	GKGCMC	1 cut(s)	2536
BseYI	CCCAGC	4 cut(s)	72, 4211, 4244, 4454
BsgI	GTGCAG	4 cut(s)	1194, 2917, 4173, 4857
Bsh1236I	CGCG	1 cut(s)	4883
Bsh1285I	CGRYCG	1 cut(s)	1622
BshFI	GGCC	9 cut(s)	57, 994, 1200, 1373, 1807, 2719, 3249, 3737, 3931
BshNI	GGYRCC	1 cut(s)	3515
BshTI	ACCGGT	1 cut(s)	548
BsiEI	CGRYCG	1 cut(s)	1622
BsiHKAI	GWGCWC	2 cut(s)	2536, 3802
BsiSI	CCGG	8 cut(s)	82, 549, 886, 1384, 1400, 3237, 4332, 4379
BslFI	GGGAC	5 cut(s)	1217, 3537, 3579, 3800, 4784
BslI	CCNNNNNNNGG	6 cut(s)	86, 987, 2065, 3242, 4023, 4618
BsmAI	GTCTC	7 cut(s)	193, 649, 1724, 1899, 2354, 2633, 4506
BsmBI	CGTCTC	2 cut(s)	193, 2354
BsmFI	GGGAC	5 cut(s)	1217, 3537, 3579, 3800, 4784
BsmI	GAATGC	2 cut(s)	885, 4681
BsnI	GGCC	9 cut(s)	57, 994, 1200, 1373, 1807, 2719, 3249, 3737, 3931
Bso31I	GGTCTC	2 cut(s)	649, 4506
Bsp1286I	GDGCHC	2 cut(s)	2536, 3802
Bsp1407I	TGTACA	2 cut(s)	777, 1714
Bsp1720I	GCTNAGC	1 cut(s)	30
Bsp19I	CCATGG	1 cut(s)	376
BspANI	GGCC	9 cut(s)	57, 994, 1200, 1373, 1807, 2719, 3249, 3737, 3931
BspFNI	CGCG	1 cut(s)	4883
BspHI	TCATGA	1 cut(s)	3499
BspMAI	CTGCAG	3 cut(s)	12, 3673, 4861
BspMI	ACCTGC	7 cut(s)	1342, 1948, 2197, 2914, 3139, 4415, 4850
BspPI	GGATC	6 cut(s)	264, 498, 1862, 3035, 3048, 3366
BspT107I	GGYRCC	1 cut(s)	3515
BspTI	CTTAAG	1 cut(s)	3104
BspTNI	GGTCTC	2 cut(s)	649, 4506
BsrBI	CCGCTC	1 cut(s)	60
BsrDI	GCAATG	6 cut(s)	58, 2378, 2988, 3000, 3681, 3717
BsrFI	RCCGGY	1 cut(s)	548
BsrGI	TGTACA	2 cut(s)	777, 1714
BssAI	RCCGGY	1 cut(s)	548
BssNI	GRCGYC	4 cut(s)	20, 225, 243, 2377
BssSI	CACGAG	3 cut(s)	1074, 2975, 3933
BssT1I	CCWWGG	4 cut(s)	376, 406, 2079, 4638
Bst2BI	CACGAG	3 cut(s)	1074, 2975, 3933
Bst6I	CTCTTC	3 cut(s)	98, 1878, 4316
BstACI	GRCGYC	4 cut(s)	20, 225, 243, 2377
BstAFI	CTTAAG	1 cut(s)	3104
BstAPI	GCANNNNNTGC	4 cut(s)	3227, 3281, 3680, 3716
BstAUI	TGTACA	2 cut(s)	777, 1714
BstC8I	GCNNGC	8 cut(s)	811, 2311, 2631, 3543, 3739, 3798, 4174, 4491
BstDSI	CCRYGG	1 cut(s)	376
BstEII	GGTNACC	1 cut(s)	3996
BstENI	CCTNNNNNAGG	1 cut(s)	4616
BstFNI	CGCG	1 cut(s)	4883
BstHHI	GCGC	2 cut(s)	4489, 4751
BstMAI	GTCTC	7 cut(s)	193, 649, 1724, 1899, 2354, 2633, 4506
BstMCI	CGRYCG	1 cut(s)	1622
BstNSI	RCATGY	3 cut(s)	1618, 3545, 4836
BstPAI	GACNNNNGTC	1 cut(s)	4518
BstPI	GGTNACC	1 cut(s)	3996
BstSFI	CTRYAG	5 cut(s)	8, 1604, 3669, 3720, 4857
BstSLI	GKGCMC	1 cut(s)	2536
BstUI	CGCG	1 cut(s)	4883
BstV2I	GAAGAC	4 cut(s)	1385, 2471, 3104, 4824
BstX2I	RGATCY	4 cut(s)	490, 2547, 3040, 3358
BstXI	CCANNNNNNTGG	2 cut(s)	3257, 4791
BstYI	RGATCY	4 cut(s)	490, 2547, 3040, 3358
Bsu36I	CCTNAGG	2 cut(s)	291, 1278
BsuI	GTATCC	4 cut(s)	1235, 2668, 2923, 3074
BsuRI	GGCC	9 cut(s)	57, 994, 1200, 1373, 1807, 2719, 3249, 3737, 3931
BtgI	CCRYGG	1 cut(s)	376
BtgZI	GCGATG	2 cut(s)	152, 1938
BtrI	CACGTC	1 cut(s)	2364
BtsI	GCAGTG	4 cut(s)	40, 1210, 2277, 4500
BveI	ACCTGC	7 cut(s)	1342, 1948, 2197, 2914, 3139, 4415, 4850
Cac8I	GCNNGC	8 cut(s)	811, 2311, 2631, 3543, 3739, 3798, 4174, 4491
CaiI	CAGNNNCTG	2 cut(s)	198, 3434
CciI	TCATGA	1 cut(s)	3499
CfoI	GCGC	2 cut(s)	4489, 4751
Cfr10I	RCCGGY	1 cut(s)	548
Cfr13I	GGNCC	9 cut(s)	993, 1331, 1371, 1502, 1940, 3551, 3736, 4049, 4117
CseI	GACGC	5 cut(s)	28, 240, 251, 2366, 4394
Csp6I	GTAC	6 cut(s)	778, 1460, 1715, 2877, 4014, 4553
CspAI	ACCGGT	1 cut(s)	548
CviAII	CATG	9 cut(s)	377, 415, 1615, 2735, 3338, 3500, 3542, 4721, 4833
CviQI	GTAC	6 cut(s)	778, 1460, 1715, 2877, 4014, 4553
DraI	TTTAAA	2 cut(s)	285, 3643
DraIII	CACNNNGTG	2 cut(s)	3227, 4811
DrdI	GACNNNNNNGTC	2 cut(s)	633, 4876
DriI	GACNNNNNGTC	1 cut(s)	95
DseDI	GACNNNNNNGTC	2 cut(s)	633, 4876
EaeI	YGGCCR	3 cut(s)	55, 1198, 3247
Eam1104I	CTCTTC	3 cut(s)	98, 1878, 4316
Eam1105I	GACNNNNNGTC	1 cut(s)	95
EarI	CTCTTC	3 cut(s)	98, 1878, 4316
EciI	GGCGGA	2 cut(s)	2768, 4350
Eco130I	CCWWGG	4 cut(s)	376, 406, 2079, 4638
Eco31I	GGTCTC	2 cut(s)	649, 4506
Eco32I	GATATC	3 cut(s)	2056, 3492, 4089
Eco47I	GGWCC	6 cut(s)	1331, 1502, 1940, 3551, 4049, 4117
Eco57I	CTGAAG	2 cut(s)	3141, 4781
Eco81I	CCTNAGG	2 cut(s)	291, 1278
Eco91I	GGTNACC	1 cut(s)	3996
EcoNI	CCTNNNNNAGG	1 cut(s)	4616
EcoO109I	RGGNCCY	2 cut(s)	1371, 1502
EcoO65I	GGTNACC	1 cut(s)	3996
EcoRI	GAATTC	1 cut(s)	1181
EcoRV	GATATC	3 cut(s)	2056, 3492, 4089
EcoT14I	CCWWGG	4 cut(s)	376, 406, 2079, 4638
EcoT22I	ATGCAT	1 cut(s)	1954
ErhI	CCWWGG	4 cut(s)	376, 406, 2079, 4638
Esp3I	CGTCTC	2 cut(s)	193, 2354
FaeI	CATG	9 cut(s)	380, 418, 1618, 2738, 3341, 3503, 3545, 4724, 4836
FaqI	GGGAC	5 cut(s)	1217, 3537, 3579, 3800, 4784
FatI	CATG	9 cut(s)	376, 414, 1614, 2734, 3337, 3499, 3541, 4720, 4832
FauI	CCCGC	2 cut(s)	73, 3746
FauNDI	CATATG	3 cut(s)	782, 1591, 1812
FbaI	TGATCA	1 cut(s)	3199
FblI	GTMKAC	1 cut(s)	2274
FspBI	CTAG	6 cut(s)	1404, 1499, 1971, 3947, 4073, 4809
FspI	TGCGCA	1 cut(s)	4488
GlaI	GCGC	2 cut(s)	4488, 4750
GsaI	CCCAGC	4 cut(s)	76, 4215, 4248, 4458
GsuI	CTGGAG	9 cut(s)	31, 175, 1687, 2195, 3045, 3831, 4338, 4530, 4614
HaeIII	GGCC	9 cut(s)	57, 994, 1200, 1373, 1807, 2719, 3249, 3737, 3931
HapII	CCGG	8 cut(s)	82, 549, 886, 1384, 1400, 3237, 4332, 4379
HgaI	GACGC	5 cut(s)	28, 240, 251, 2366, 4394
HhaI	GCGC	2 cut(s)	4489, 4751
Hin1I	GRCGYC	4 cut(s)	20, 225, 243, 2377
Hin1II	CATG	9 cut(s)	380, 418, 1618, 2738, 3341, 3503, 3545, 4724, 4836
Hin6I	GCGC	2 cut(s)	4487, 4749
HinP1I	GCGC	2 cut(s)	4487, 4749
HincII	GTYRAC	2 cut(s)	211, 4147
HindII	GTYRAC	2 cut(s)	211, 4147
HindIII	AAGCTT	1 cut(s)	3419
HpaII	CCGG	8 cut(s)	82, 549, 886, 1384, 1400, 3237, 4332, 4379
Hpy166II	GTNNAC	9 cut(s)	211, 871, 2275, 2534, 3394, 4039, 4120, 4147, 4163
Hpy8I	GTNNAC	9 cut(s)	211, 871, 2275, 2534, 3394, 4039, 4120, 4147, 4163
Hpy99I	CGWCG	5 cut(s)	101, 104, 113, 230, 245
HpyCH4IV	ACGT	4 cut(s)	225, 2363, 3163, 4165
HpySE526I	ACGT	4 cut(s)	225, 2363, 3163, 4165
Hsp92I	GRCGYC	4 cut(s)	20, 225, 243, 2377
Hsp92II	CATG	9 cut(s)	380, 418, 1618, 2738, 3341, 3503, 3545, 4724, 4836
HspAI	GCGC	2 cut(s)	4487, 4749
Ksp22I	TGATCA	1 cut(s)	3199
MaeI	CTAG	6 cut(s)	1404, 1499, 1971, 3947, 4073, 4809
MaeII	ACGT	4 cut(s)	225, 2363, 3163, 4165
MbiI	CCGCTC	1 cut(s)	60
MfeI	CAATTG	6 cut(s)	502, 2433, 3251, 3368, 4098, 4392
MflI	RGATCY	4 cut(s)	490, 2547, 3040, 3358
MhlI	GDGCHC	2 cut(s)	2536, 3802
MlsI	TGGCCA	1 cut(s)	3249
MluNI	TGGCCA	1 cut(s)	3249
MmeI	TCCRAC	9 cut(s)	186, 208, 322, 535, 1918, 2872, 3032, 3203, 4276
Mox20I	TGGCCA	1 cut(s)	3249
Mph1103I	ATGCAT	1 cut(s)	1954
MroXI	GAANNNNTTC	3 cut(s)	526, 2124, 2663
MscI	TGGCCA	1 cut(s)	3249
MslI	CAYNNNNRTG	7 cut(s)	549, 1099, 2993, 3504, 3546, 4044, 4258
Msp20I	TGGCCA	1 cut(s)	3249
MspA1I	CMGCKG	4 cut(s)	248, 3205, 4798, 4856
MspCI	CTTAAG	1 cut(s)	3104
MspI	CCGG	8 cut(s)	82, 549, 886, 1384, 1400, 3237, 4332, 4379
MunI	CAATTG	6 cut(s)	502, 2433, 3251, 3368, 4098, 4392
Mva1269I	GAATGC	2 cut(s)	885, 4681
MvnI	CGCG	1 cut(s)	4883
NciI	CCSGG	4 cut(s)	82, 1400, 3237, 4379
NcoI	CCATGG	1 cut(s)	376
NdeI	CATATG	3 cut(s)	782, 1591, 1812
NlaIII	CATG	9 cut(s)	380, 418, 1618, 2738, 3341, 3503, 3545, 4724, 4836
NmuCI	GTSAC	9 cut(s)	16, 926, 1022, 1772, 2946, 3172, 4196, 4348, 4867
NsbI	TGCGCA	1 cut(s)	4488
NsiI	ATGCAT	1 cut(s)	1954
NspI	RCATGY	3 cut(s)	1618, 3545, 4836
OliI	CACNNNNGTG	1 cut(s)	549
PaeI	GCATGC	1 cut(s)	3545
PagI	TCATGA	1 cut(s)	3499
PaqCI	CACCTGC	1 cut(s)	4850
PasI	CCCWGGG	2 cut(s)	486, 3555
PcsI	WCGNNNNNNNCGW	4 cut(s)	20, 93, 105, 2374
PctI	GAATGC	2 cut(s)	885, 4681
PdmI	GAANNNNTTC	3 cut(s)	526, 2124, 2663
PfeI	GAWTC	2 cut(s)	1225, 1472
PflFI	GACNNNGTC	2 cut(s)	1261, 2644
PfoI	TCCNGGA	3 cut(s)	191, 1504, 3808
PinAI	ACCGGT	1 cut(s)	548
Ple19I	CGATCG	1 cut(s)	1622
PpuMI	RGGWCCY	1 cut(s)	1502
PshAI	GACNNNNGTC	1 cut(s)	4518
PshBI	ATTAAT	1 cut(s)	1038
Psp5II	RGGWCCY	1 cut(s)	1502
PspEI	GGTNACC	1 cut(s)	3996
PspFI	CCCAGC	4 cut(s)	72, 4211, 4244, 4454
PspPI	GGNCC	9 cut(s)	993, 1331, 1371, 1502, 1940, 3551, 3736, 4049, 4117
PspPPI	RGGWCCY	1 cut(s)	1502
PsrI	GAACNNNNNNTAC	2 cut(s)	2904, 2936
PstI	CTGCAG	3 cut(s)	12, 3673, 4861
PstNI	CAGNNNCTG	2 cut(s)	198, 3434
PsuI	RGATCY	4 cut(s)	490, 2547, 3040, 3358
PsyI	GACNNNGTC	2 cut(s)	1261, 2644
PvuI	CGATCG	1 cut(s)	1622
PvuII	CAGCTG	3 cut(s)	3205, 4798, 4856
RsaI	GTAC	6 cut(s)	779, 1461, 1716, 2878, 4015, 4554
RsaNI	GTAC	6 cut(s)	778, 1460, 1715, 2877, 4014, 4553
RseI	CAYNNNNRTG	7 cut(s)	549, 1099, 2993, 3504, 3546, 4044, 4258
Sau96I	GGNCC	9 cut(s)	993, 1331, 1371, 1502, 1940, 3551, 3736, 4049, 4117
SduI	GDGCHC	2 cut(s)	2536, 3802
SfcI	CTRYAG	5 cut(s)	8, 1604, 3669, 3720, 4857
SgrAI	CRCCGGYG	1 cut(s)	548
SinI	GGWCC	6 cut(s)	1331, 1502, 1940, 3551, 4049, 4117
SmiMI	CAYNNNNRTG	7 cut(s)	549, 1099, 2993, 3504, 3546, 4044, 4258
SmlI	CTYRAG	2 cut(s)	2248, 3104
SmoI	CTYRAG	2 cut(s)	2248, 3104
SphI	GCATGC	1 cut(s)	3545
SspI	AATATT	4 cut(s)	619, 1794, 1927, 4778
SspMI	CTAG	6 cut(s)	1404, 1499, 1971, 3947, 4073, 4809
StyI	CCWWGG	4 cut(s)	376, 406, 2079, 4638
TaiI	ACGT	4 cut(s)	228, 2366, 3166, 4168
TaqI	TCGA	7 cut(s)	102, 128, 798, 1876, 2185, 3654, 3802
TaqII	GACCGA	1 cut(s)	196
TatI	WGTACW	2 cut(s)	777, 1714
TauI	GCSGC	7 cut(s)	60, 79, 158, 248, 1203, 3639, 4743
TfiI	GAWTC	2 cut(s)	1225, 1472
TseFI	GTSAC	9 cut(s)	16, 926, 1022, 1772, 2946, 3172, 4196, 4348, 4867
Tsp45I	GTSAC	9 cut(s)	16, 926, 1022, 1772, 2946, 3172, 4196, 4348, 4867
TspGWI	ACGGA	2 cut(s)	102, 1571
Tth111I	GACNNNGTC	2 cut(s)	1261, 2644
Vha464I	CTTAAG	1 cut(s)	3104
VneI	GTGCAC	1 cut(s)	2532
VpaK11BI	GGWCC	6 cut(s)	1331, 1502, 1940, 3551, 4049, 4117
VspI	ATTAAT	1 cut(s)	1038
XagI	CCTNNNNNAGG	1 cut(s)	4616
XapI	RAATTY	9 cut(s)	146, 1122, 1181, 2398, 2452, 2487, 2695, 3295, 3919
XceI	RCATGY	3 cut(s)	1618, 3545, 4836
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1596
XmiI	GTMKAC	1 cut(s)	2274
XmnI	GAANNNNTTC	3 cut(s)	526, 2124, 2663
XspI	CTAG	6 cut(s)	1404, 1499, 1971, 3947, 4073, 4809
ZraI	GACGTC	1 cut(s)	226
Zsp2I	ATGCAT	1 cut(s)	1954

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.