Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0005346

Physical Location & Seq

Genomic Coordinates Reverse (-)

51990715 .. 51998307

Gene Structure 7593 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00035740.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 564 bp

ATGCCCCGGGTTCGTTGTTTGGGTGGCGGAAGGAGGAAATTGGCCGGAAATCAAAGGGAGGCCGAAAGGAGAGAGCTCGGGGAAGAAGAGAGAGAAAGTTACCGAGTTGGGTGGATTTCCGAAAATGGAAATCTACCAACATCATTGATCACTAAAACAGTTGGCGCAAAAAGTTTCAGATTCCCGCCTAATCATTTGAGTGACCAAGCTAGGTCTGGGCACTACATGGCATTTGGTTACTCGAGTGAGGATGTCCAAATGGTTATTGAGACAATGGCTTCACAAGGGAAGGAGCCTACATTTTGCATGGGCAATGATATTCCATTGGCGATTTTATCTCAGAGACCACATATGCTATATGACTATTTCAAGCAACGGTTTGTGCAAGTTATCTTGTCCAGTCCTGTGTTCAATGAAGGAGAACTTGAGTCATTGCTTAAGGACGCTCAGCTAAAAGCCCAAGTTTTACCTACATTTTTTGATATTCATAAAGGGGTCGATGGTTCCTTGGAAAAAAAGCTGTATAAGCTTTGTGAAGCTGCTGATGAAGTCTACTGCAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009853 GO:0009941 GO:0009987 GO:0010033 GO:0015930 GO:0016020 GO:0016041 GO:0016053 GO:0016491 GO:0016638 GO:0016643 GO:0019222 GO:0019676 GO:0019740 GO:0019752 GO:0019899 GO:0031323 GO:0031967 GO:0031974 GO:0031975 GO:0034285 GO:0042221 GO:0043085 GO:0043094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0043650 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048046 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051338 GO:0051347 GO:0055114 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0080114 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00630 ko00910 ko01120 map00630 map00910 map01120

Pfam Domains

Protein Families

GATase_2 GXGXG Glu_syn_central Glu_synthase

Protein Analysis

187

Amino Acids

21.16

Weight (kDa)

6.44

Isoelectric Point (pI)

51.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glu_syn_central	PF04898	75 - 130	1.6e-20	Glutamate synthase central domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0005346)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41220 AT5G04140 AT5G04140
fragaria_vesca	FvH4_6g25460 FvH4_6g25460 FvH4_6g25460 FvH4_6g25460
malus_domestica	MD14G1087500.v1.1
prunus_persica	Prupe.7G039100_v2.0.a1
pyrus_communis	pycom14g07760 pycom14g07770
rosa_chinensis	RchiOBHm_Chr2g0125471
rosa_laevigata	RLG00000018438 RLG00000018867 RLG00000030615 RLG00000034746
rosa_multiflora	Rmu_sc0005339.1_g000009 Rmu_sc0005339.1_g000011 Rmu_ssc0000126.1_g000014
rosa_roxburghii	Rroxscaffold_1G00035740 Rroxscaffold_2G00117960
rosa_rugosa	Rorug02G0257500 Rorug02G0257600
rosa_samantha	Rh2AG316500 Rh2BG324000 Rh2BG355400 Rh2CG306200 Rh2DG342600
rosa_wichuraiana	Rw2G025530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	552
AciI	CCGC	2 cut(s)	27, 185
AcoI	YGGCCR	1 cut(s)	42
AflII	CTTAAG	1 cut(s)	437
AgsI	TTSAA	2 cut(s)	370, 412
AluBI	AGCT	7 cut(s)	76, 209, 451, 520, 529, 539, 561
AluI	AGCT	7 cut(s)	76, 209, 451, 520, 529, 539, 561
Alw21I	GWGCWC	1 cut(s)	78
Alw26I	GTCTC	2 cut(s)	263, 337
Ama87I	CYCGRG	3 cut(s)	6, 77, 241
AoxI	GGCC	2 cut(s)	42, 60
ApeKI	GCWGC	2 cut(s)	539, 558
AspLEI	GCGC	1 cut(s)	167
AsuC2I	CCSGG	2 cut(s)	7, 8
AvaI	CYCGRG	3 cut(s)	6, 77, 241
BaeGI	GKGCMC	1 cut(s)	222
BanII	GRGCYC	1 cut(s)	78
Bbv12I	GWGCWC	1 cut(s)	78
BbvI	GCAGC	1 cut(s)	526
BccI	CCATC	1 cut(s)	494
BclI	TGATCA	1 cut(s)	147
BcnI	CCSGG	2 cut(s)	7, 8
BcoDI	GTCTC	2 cut(s)	263, 337
BfaI	CTAG	1 cut(s)	210
BfmI	CTRYAG	1 cut(s)	556
BfrI	CTTAAG	1 cut(s)	437
BisI	GCNGC	2 cut(s)	540, 559
BlpI	GCTNAGC	1 cut(s)	447
BlsI	GCNGC	2 cut(s)	541, 560
Bme1390I	CCNGG	2 cut(s)	7, 8
BmeT110I	CYCGRG	3 cut(s)	6, 77, 241
BmiI	GGNNCC	2 cut(s)	294, 505
BmrFI	CCNGG	2 cut(s)	7, 8
Bpu1102I	GCTNAGC	1 cut(s)	447
BpuEI	CTTGAG	1 cut(s)	446
BpuMI	CCSGG	2 cut(s)	7, 8
BsaI	GGTCTC	1 cut(s)	337
BsaJI	CCNNGG	3 cut(s)	5, 6, 507
Bse1I	ACTGG	1 cut(s)	399
Bse3DI	GCAATG	2 cut(s)	319, 431
BseDI	CCNNGG	3 cut(s)	5, 6, 507
BseGI	GGATG	1 cut(s)	256
BseMI	GCAATG	2 cut(s)	319, 431
BseMII	CTCAG	2 cut(s)	353, 461
BseNI	ACTGG	1 cut(s)	399
BseSI	GKGCMC	1 cut(s)	222
BseXI	GCAGC	1 cut(s)	526
BshFI	GGCC	2 cut(s)	44, 62
BsiHKAI	GWGCWC	1 cut(s)	78
BsiHKCI	CYCGRG	3 cut(s)	6, 77, 241
BsiSI	CCGG	2 cut(s)	7, 45
BsmAI	GTCTC	2 cut(s)	263, 337
BsnI	GGCC	2 cut(s)	44, 62
Bso31I	GGTCTC	1 cut(s)	337
BsoBI	CYCGRG	3 cut(s)	6, 77, 241
Bsp1286I	GDGCHC	2 cut(s)	78, 222
Bsp143I	GATC	1 cut(s)	147
Bsp1720I	GCTNAGC	1 cut(s)	447
BspACI	CCGC	2 cut(s)	27, 185
BspANI	GGCC	2 cut(s)	44, 62
BspCNI	CTCAG	2 cut(s)	352, 460
BspLI	GGNNCC	2 cut(s)	294, 505
BspMAI	CTGCAG	1 cut(s)	560
BspTI	CTTAAG	1 cut(s)	437
BspTNI	GGTCTC	1 cut(s)	337
BsrDI	GCAATG	2 cut(s)	319, 431
BsrI	ACTGG	1 cut(s)	399
BssECI	CCNNGG	3 cut(s)	5, 6, 507
BssMI	GATC	1 cut(s)	147
BssT1I	CCWWGG	1 cut(s)	507
Bst4CI	ACNGT	2 cut(s)	160, 378
Bst6I	CTCTTC	1 cut(s)	81
BstAFI	CTTAAG	1 cut(s)	437
BstDEI	CTNAG	2 cut(s)	339, 447
BstF5I	GGATG	1 cut(s)	256
BstHHI	GCGC	1 cut(s)	167
BstKTI	GATC	1 cut(s)	150
BstMAI	GTCTC	2 cut(s)	263, 337
BstMBI	GATC	1 cut(s)	147
BstMWI	GCNNNNNNNGC	1 cut(s)	526
BstSCI	CCNGG	2 cut(s)	5, 6
BstSFI	CTRYAG	1 cut(s)	556
BstSLI	GKGCMC	1 cut(s)	222
BstV1I	GCAGC	1 cut(s)	526
BsuRI	GGCC	2 cut(s)	44, 62
BtsCI	GGATG	1 cut(s)	256
CfoI	GCGC	1 cut(s)	167
Cfr9I	CCCGGG	1 cut(s)	6
CseI	GACGC	1 cut(s)	452
CviAII	CATG	2 cut(s)	226, 307
DdeI	CTNAG	2 cut(s)	339, 447
DpnI	GATC	1 cut(s)	149
DpnII	GATC	1 cut(s)	147
EaeI	YGGCCR	1 cut(s)	42
Eam1104I	CTCTTC	1 cut(s)	81
EarI	CTCTTC	1 cut(s)	81
EciI	GGCGGA	1 cut(s)	42
Ecl136II	GAGCTC	1 cut(s)	76
Eco130I	CCWWGG	1 cut(s)	507
Eco24I	GRGCYC	1 cut(s)	78
Eco31I	GGTCTC	1 cut(s)	337
Eco53kI	GAGCTC	1 cut(s)	76
Eco88I	CYCGRG	3 cut(s)	6, 77, 241
EcoICRI	GAGCTC	1 cut(s)	76
EcoT14I	CCWWGG	1 cut(s)	507
EcoT38I	GRGCYC	1 cut(s)	78
ErhI	CCWWGG	1 cut(s)	507
FaeI	CATG	2 cut(s)	229, 310
FaiI	YATR	8 cut(s)	227, 308, 351, 353, 358, 360, 489, 525
FalI	AAGNNNNNCTT	2 cut(s)	408, 440
FatI	CATG	2 cut(s)	225, 306
FauI	CCCGC	1 cut(s)	192
FauNDI	CATATG	1 cut(s)	351
FbaI	TGATCA	1 cut(s)	147
FblI	GTMKAC	1 cut(s)	552
Fnu4HI	GCNGC	2 cut(s)	540, 559
FokI	GGATG	1 cut(s)	263
FriOI	GRGCYC	1 cut(s)	78
Fsp4HI	GCNGC	2 cut(s)	540, 559
FspBI	CTAG	1 cut(s)	210
GlaI	GCGC	1 cut(s)	166
GluI	GCNGC	2 cut(s)	540, 559
HaeIII	GGCC	2 cut(s)	44, 62
HapII	CCGG	2 cut(s)	7, 45
HgaI	GACGC	1 cut(s)	452
HhaI	GCGC	1 cut(s)	167
Hin1II	CATG	2 cut(s)	229, 310
Hin6I	GCGC	1 cut(s)	165
HinP1I	GCGC	1 cut(s)	165
HindIII	AAGCTT	1 cut(s)	527
HinfI	GANTC	2 cut(s)	180, 428
HpaII	CCGG	2 cut(s)	7, 45
Hpy166II	GTNNAC	1 cut(s)	553
Hpy188I	TCNGA	3 cut(s)	121, 179, 342
Hpy8I	GTNNAC	1 cut(s)	553
HpyAV	CCTTC	3 cut(s)	24, 283, 410
HpyCH4III	ACNGT	2 cut(s)	160, 378
HpyCH4V	TGCA	3 cut(s)	306, 385, 558
HpyF10VI	GCNNNNNNNGC	1 cut(s)	526
HpyF3I	CTNAG	2 cut(s)	339, 447
Hsp92II	CATG	2 cut(s)	229, 310
HspAI	GCGC	1 cut(s)	165
Ksp22I	TGATCA	1 cut(s)	147
Kzo9I	GATC	1 cut(s)	147
LmnI	GCTCC	1 cut(s)	292
LpnPI	CCDG	5 cut(s)	20, 58, 201, 412, 417
Lsp1109I	GCAGC	1 cut(s)	526
MaeI	CTAG	1 cut(s)	210
MaeIII	GTNAC	3 cut(s)	98, 200, 236
MalI	GATC	1 cut(s)	149
MboI	GATC	1 cut(s)	147
MboII	GAAGA	2 cut(s)	95, 98
MhlI	GDGCHC	2 cut(s)	78, 222
MluCI	AATT	1 cut(s)	38
MlyI	GAGTC	1 cut(s)	437
MnlI	CCTC	3 cut(s)	27, 52, 241
MseI	TTAA	1 cut(s)	438
MslI	CAYNNNNRTG	1 cut(s)	198
MspCI	CTTAAG	1 cut(s)	437
MspI	CCGG	2 cut(s)	7, 45
MspR9I	CCNGG	2 cut(s)	7, 8
MwoI	GCNNNNNNNGC	1 cut(s)	526
NciI	CCSGG	2 cut(s)	7, 8
NdeI	CATATG	1 cut(s)	351
NdeII	GATC	1 cut(s)	147
NlaIII	CATG	2 cut(s)	229, 310
NlaIV	GGNNCC	2 cut(s)	294, 505
NmuCI	GTSAC	1 cut(s)	200
PaeR7I	CTCGAG	1 cut(s)	241
PfeI	GAWTC	1 cut(s)	180
PkrI	GCNGC	2 cut(s)	541, 560
PleI	GAGTC	1 cut(s)	436
PpsI	GAGTC	1 cut(s)	436
Psp124BI	GAGCTC	1 cut(s)	78
PspN4I	GGNNCC	2 cut(s)	294, 505
PspXI	VCTCGAGB	1 cut(s)	241
PstI	CTGCAG	1 cut(s)	560
RseI	CAYNNNNRTG	1 cut(s)	198
SacI	GAGCTC	1 cut(s)	78
SaqAI	TTAA	1 cut(s)	438
SatI	GCNGC	2 cut(s)	540, 559
Sau3AI	GATC	1 cut(s)	147
SchI	GAGTC	1 cut(s)	437
ScrFI	CCNGG	2 cut(s)	7, 8
SduI	GDGCHC	2 cut(s)	78, 222
SetI	ASST	9 cut(s)	78, 211, 215, 453, 472, 522, 531, 541, 563
SfcI	CTRYAG	1 cut(s)	556
Sfr274I	CTCGAG	1 cut(s)	241
SlaI	CTCGAG	1 cut(s)	241
SmaI	CCCGGG	1 cut(s)	8
SmiMI	CAYNNNNRTG	1 cut(s)	198
SmlI	CTYRAG	3 cut(s)	241, 425, 437
SmoI	CTYRAG	3 cut(s)	241, 425, 437
Sse9I	AATT	1 cut(s)	38
SsiI	CCGC	2 cut(s)	27, 185
SspMI	CTAG	1 cut(s)	210
SstI	GAGCTC	1 cut(s)	78
StyD4I	CCNGG	2 cut(s)	5, 6
StyI	CCWWGG	1 cut(s)	507
TaaI	ACNGT	2 cut(s)	160, 378
TaqI	TCGA	2 cut(s)	242, 498
TasI	AATT	1 cut(s)	38
TfiI	GAWTC	1 cut(s)	180
Tru1I	TTAA	1 cut(s)	438
Tru9I	TTAA	1 cut(s)	438
TseFI	GTSAC	1 cut(s)	200
TseI	GCWGC	2 cut(s)	539, 558
Tsp45I	GTSAC	1 cut(s)	200
TspDTI	ATGAA	3 cut(s)	429, 476, 561
TspMI	CCCGGG	1 cut(s)	6
Vha464I	CTTAAG	1 cut(s)	437
XcmI	CCANNNNNNNNNTGG	1 cut(s)	212
XhoI	CTCGAG	1 cut(s)	241
XmaI	CCCGGG	1 cut(s)	6
XmiI	GTMKAC	1 cut(s)	552
XspI	CTAG	1 cut(s)	210

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.