Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0005346

Physical Location & Seq

Genomic Coordinates Forward (+)

48768434 .. 48793942

Gene Structure 25509 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00117960.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5223 bp

ATGGCCGCGTTGCAGTCACTGGCTCCTGTTCCTCAGCTGCTTCACTCCTCCAATGGCCGCTCCCCGACGAAAGGACTCTTTCACGTCGATTTCGTCGGCTACTGCAAATCAAAGAGGAGTCGCCGTAAATTCAACGGAGCATCCCAGCCTCTACGGACAAGCTTCCCTCAATTCGTTTCCAAAGCCGTTCTCGACCTTGGACCCTCCTCTACCGATCACTCAACTCCTCCTCCTCCTCCTCCTACTTCCAAACCCCAGGTTGCGGACCTGAAAGATATAATATCAGAAAGAGGAGCTTGCGGAGTTGGATTCATTGCCAATCTGGACAACAAAGCATCTCATGGCATTGTTAAGGATGCTCTTACAGCTCTTGGCTGCATGGAACATCGTGGAGGCTGTGGAGCAGATAATGACTCTGGTGATGGTTCAGGATTGATGAGTTCAATCCCATGGGATCTATTTAACAATTGGGCTGACAAGCAAGGGATTGCATCGTTTGACAAGTTACACACTGGTGTTGGAATGATTTTCCTCCCCAAAGAGGACAACCTTATGAAAGAAGCCAAAAAAGCTATTGTGAATATTTTTAAACAAGAGGGCCTTGAAGTGCTTGGGTGGAGACCTGTTCCAGTGAATACAGCTATAGTTGGTTTCTATGCAAAAGAAACAATGCCCAGCATACAACAGGTATTCGTCAAAGTTGTTAAAGAGGAAAAAGTTGAGAACATTGAGCGAGAACTGTACATATGCCGGAAGTTAATCGAAAGAGCAGCAAGCTCAGAAAGTTGGGGCAGTGATCTTTATTTCTGTTCTTTGTCCAATCAAACAATAGTTTACAAAGGAATGCTACGCTCAGAAGTTCTTGGTTTATTCTATTCTGACCTCCAAAGTGACCTTTATAAATCCGCTTTTGCCATCTATCATCGAAGGTATAGCACAAATACCACTCCTAGATGGCCCCTTGCACAACCAATGAGGTTACTTGGTCACAACGGAGAGATCAACACCATACAGGGGAACTTGAACTGGATGCAATCACGTGAAGCCTCATTGAAGTCACCTGTATGGCATGATCGTGAAAATGAACTTCGTCCATATGGCAATCCAAAGGGTTCAGACTCTGCGAATCTTGACAGTGCGGCTGAATTCTTACTAAGAAGTGGCCGGACTGCTGAGGAGGCTCTGATGATTCTTGTCCCTGAGGGATACAAAAATCATCCAACTCTGATGATTAACTATCCAGAGGTGGTTGATTTTTATGATTATTACAAGGGTCAGATGGAGGCTTGGGATGGACCTGCTTTATTGTTATTCAGTGATGGAAAAACAGTTGGGGCATGTCTTGATCGTAATGGACTTCGGCCTGCTAGATATTGGCGGACATCTGATAACGTTGTTTATGTCGCATCTGAGGTTGGGGTTCTTCCAGTGGATGATTCAAAAGTTACAATGAAAGGTCGTCTAGGTCCTGGAATGATGATATCTGTTGACTTACTAAGTGGTCAGGTGGATTTTGGTTGTGAAGAAACTATGCTTATGAGGCATTGGAGCCAGCAGGAGTTAGTAGGTGATCCAACACCTGAAATAAAAGGTGCAGTAGACTTTAGCACCTGTTGGAGAGAATTAAGTGGTTTTGATGCAGAGAAGGGAATTAGGAATGTAATGAGGGATAGACCTGATTTGGAATTCGAAGGCACACCCCAGGCTTTTGGTGGCTTGCGTGAGTCAGTGTCAATACTTGCAATGGGGTACAAAAGAAAATGCTTTATGTCTGGTCTTCCACACACCTTTCAAGGCTTTGGGGAAGGGTACTCTGTAGAGATGCTACTGAGGGTTTCTGTCTCAAGTAAAATTTTCAATTTGCTAGCTGTGGTTTATGAGAACACAGAAGTTAAGAAGCGAGTGGCTTTGTCAAATCCTTATGGAAAGTGGGTGCAGGAAAACATGCGGACCTTAAAGACTGTGAACTTCCTTTCATCCACAATCGCGGACAATGATGCAATACTGAGACGGCAACAGGCATTTGGTTACTCGAGTGAGGATGTCCAAATGGTTATCGAGACAATGGCTTCACAAGGGAAGGAGCCTACATTTTGCATGGGCGATGATATTCCATTGGCGATTTTATCTCAGAGACCACATATGCTATATGACTATTTCAAGCAACGGTTTGCGCAGGTTACAAATCCAGCCATTGATCCTCTAAGAGAAGGGTTAGTTATGTCTCTTGAAGTCAACATTGGAAAGCGACAAAATATTTTGGAGGTTGGACCTGAAAATGCATCGCAGGTTATCTTGTCCAGTCCTGTGCTCAATGAAGGAGAACTTGAGTCATTGCTTAAGGATGCTCAGCTAAAACCCCAAGTTTTACCTACATTTTTTGATATTCATAAAGGGGTTGATGGTTCCTTGGAAAAAACGCTGTATAAGCTTTGTGAAGCTGCTGATGATGCTGTTCAGAATGGTTGCCAATTGCTTGTTCTGTCTGACCGGTCTGATGAGTTGGTGAGGATCCAATTTAGATTGGCATTGCTCTCTATAGATATTAAAGCTTTTAATGATTCTGCGATGATTACCCTTTTACTTCAGGAAGCAACTCGTCCTGCAATTCCAATCCTTCTTGCTGTTGGTGCTGTTCATCAGCATCTTATTCAAAATGGGTTGAGAATGTCAGCTTCTATAATAGTGGATACTGCTCAGTGCTTTAGCACTCATCAATTTGCCTGTTTGATTGGATATGGTGCAAGTGCTGTGTGTCCATACTTGGCATTGGAGACATGTCGGCAGTGGCGTCTGAGTAATAAAACAGTAAATCTAATGCGGAACGGGAAGATGCCTACTGTAACGATTGAACAGGCTCAAAAGAACTTCTGCAAGGCTGTTACGGCTGGTCTTCTTAAAATTCTTTCCAAAATGGGGATCTCGTTGCTTTCAAGTTATTGCGGTGCACAGATTTTTGAGATTTATGGATTGGGGAAGGAGGTTGTTGATCTTGCATTTTGTGGGAGTATATCAAGTATTGGTGGATTAACTTTTGATGAGCTGGCGAGGGAGACTTTGTCCTTCTGGGTGAAGGCTTTCTCTGAGGATACAGCTAAACGACTGGAAAATTTTGGATTTATACAATTCAGACCAGGAGGGGAGTATCATGGAAACAACCCAGAGATGTCGAAATTGCTACACAAAGCTGTTCGACAGAAGAATGAAAGTGCCTTTTCTGTTTATCAGCAGCATTTAGCTAACCGGCCCGTTAATGTTCTTCGCGATCTCATTGAGTTCAAAAGCGATCGTGCTCCAATCCCTGTGGGAAAGGTTGAACCTGCTGTATCCATTGTTCAACGATTCTGCACTGGTGGGATGTCACTTGGAGCTATTTCCAGAGAAACTCATGAGGCTATAGCCATTGCAATGAATAGAATAGGTGGAAAGTCCAATTCTGGAGAAGGTGGTGAGGATCCTATTCGCTGGAAACCACTTGCAGATGTTGTTGATGGGTATTCACCGACCCTTCCACATCTAAAAGGTCTTCAAAATGGAGATACTGCTACAAGTGCTATCAAGCAGGTTGCTTCAGGACGTTTTGGAGTCACTCCAACCTTCTTGGTCAATGCCGATCAGCTGGAAATCAAAATTGCACAAGGGGCGAAGCCTGGTGAAGGTGGCCAACTGCCTGGTAAAAAAGTTAGTGCATACATTGCGAGGTTGAGAAACTCAAAACCTGGGGTTCCACTTATATCTCCACCCCCACACCATGACATTTATTCTATAGAGGATCTTGCCCAATTGATCTATGACCTTCATCAGGTGAACCCTAAAGCTAAGGTGTCAGTGAAACTTGTGGCTGAAGCTGGAATTGGCACTGTTGCTTCTGGGGTCGCAAAGGGTAATGCTGATATCATACAGATATCAGGGCATGATGGTGGAACCGGGGCCAGTCCTATAAGTTCCATTAAGCATGCTGGTGGGCCCTGGGAACTAGGACTCACAGAAACACACCAGACACTCATAGAAAATGGACTTAGAGAACGAGTCATTCTTAGAGTTGATGGTGGCTTTAAAAGCGGAGTTGATGTCCTAATGGCTGCAACTATGGGTGCTGATGAGTACGGGTTTGGGTCTGTGGCAATGATTGCTACAGGTTGTGTTATGGCCCGCATTTGCCATACAAATAATTGTCCTGTTGGTGTAGCCAGCCAGGTAAGAGGCATGTTGGCACAGTTGGGATATGAGAAGTTAGACGATATAATTGGACGGACAGATTTGTTTAGACCACGAGATATATCTCTAATGAAAACACAGCATCTTGATCTCGGTTATATTCTATCAAATGTCGGGTTACCAAAATGGAGCAGTACCATGATCAGGAATCAGGATGTTCATACTAACGGTCCTGTTCTGGATGATATTTTACTAGGAGATCCAGAGATATCAGATGCAATTGAGAATGAAAAAGTGGTTCAGAAGACCATAAAGATATACAATGTGGATCGTGCTGTTTGTGGGCGCATAGCAGGGGTGGTTGCCAAAAAGTATGGTGACACTGGTTTTGCTGGGCAGCTGAACATAACCTTTACAGGGAGCGCTGGGCAATCATTTGGTTGCTTCTTGACTCCTGGGATGAATATTCGACTTGTTGGAGAGGCAAATGATTATGTGGGAAAGTATTTCGGGGGAGAATTGGTTGTCACTCCGGCAGAGAATACGGGATTTTGCCCTGAGGATGCAACTATTGTAGGGAATACCTGCTTGTATGGTGCAACTGGGGGTCAAATCTTTGTTAGAGGGAAAGCCGGAGAGCGATTTGCTGTAAGAAACTCTCTTGCGCAAGCAGTGGTTGAAGGCACTGGAGACCACTGTTGTGAATACATGACTGGTGGCTGTGTAGTTGTACTTGGAAAAGTGGGTAGAAATGTTGCTGCTGGAATGACTGGAGGTCTGGCATACTTTCTTGATGAGGACGATTCTTTCATCCCCAAGGTAAATAGGGAGATTGTGAAGATCCAAAGAGTCAATGCTCCTGTGGGGCAGATGCAGCTAAAGAGCCTCATTGAAGCCCATGTTGAAAAAACTGGAAGTGGCAAGGGCTCTGTTATTCTGAAAGAGTGGGACAAGTACTTGCCACTCTTCTGGCAGCTGGTTCCCCCTAGTGAAGAAGACACTCCAGAGGCGTGTGCAGACTATGTGAGAACAGCTGCTGGTGAAGTGACTTTGCAGTCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009853 GO:0009941 GO:0009987 GO:0010033 GO:0015930 GO:0016020 GO:0016041 GO:0016053 GO:0016491 GO:0016638 GO:0016643 GO:0019222 GO:0019676 GO:0019740 GO:0019752 GO:0019899 GO:0031323 GO:0031967 GO:0031974 GO:0031975 GO:0034285 GO:0042221 GO:0043085 GO:0043094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0043650 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048046 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051338 GO:0051347 GO:0055114 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0080114 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00630 ko00910 ko01120 map00630 map00910 map01120

Pfam Domains

Protein Families

GATase_2 GXGXG Glu_syn_central Glu_synthase

Protein Analysis

1740

Amino Acids

189.89

Weight (kDa)

6.27

Isoelectric Point (pI)

35.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	100 - 503	4.3e-170	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	670 - 991	2e-100	Glutamate synthase central domain
Glu_synthase	PF01645	1051 - 1404	1.2e-144	Conserved region in glutamate synthase
GXGXG	PF01493	1491 - 1673	3e-75	GXGXG motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005346)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41220 AT5G04140 AT5G04140
fragaria_vesca	FvH4_6g25460 FvH4_6g25460 FvH4_6g25460 FvH4_6g25460
malus_domestica	MD14G1087500.v1.1
prunus_persica	Prupe.7G039100_v2.0.a1
pyrus_communis	pycom14g07760 pycom14g07770
rosa_chinensis	RchiOBHm_Chr2g0125471
rosa_laevigata	RLG00000018438 RLG00000018867 RLG00000030615 RLG00000034746
rosa_multiflora	Rmu_sc0005339.1_g000009 Rmu_sc0005339.1_g000011 Rmu_ssc0000126.1_g000014
rosa_roxburghii	Rroxscaffold_1G00035740 Rroxscaffold_2G00117960
rosa_rugosa	Rorug02G0257500 Rorug02G0257600
rosa_samantha	Rh2AG316500 Rh2BG324000 Rh2BG355400 Rh2CG306200 Rh2DG342600
rosa_wichuraiana	Rw2G025530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	900
AasI	GACNNNNNNGTC	1 cut(s)	5212
Acc16I	TGCGCA	2 cut(s)	2175, 4824
Acc36I	ACCTGC	6 cut(s)	1306, 2167, 2278, 3328, 3553, 4751
AccBSI	CCGCTC	1 cut(s)	60
AccI	GTMKAC	1 cut(s)	1599
AccII	CGCG	3 cut(s)	8, 1988, 3264
AclI	AACGTT	1 cut(s)	1392
AcoI	YGGCCR	4 cut(s)	3, 55, 1162, 3661
AcsI	RAATTY	6 cut(s)	128, 1145, 1685, 1851, 2901, 3109
AcuI	CTGAAG	3 cut(s)	2570, 3555, 3864
AcvI	CACGTG	1 cut(s)	1040
AcyI	GRCGYC	1 cut(s)	2791
AfaI	GTAC	7 cut(s)	743, 1751, 1811, 4106, 4354, 4890, 5114
AfeI	AGCGCT	1 cut(s)	4582
AfiI	CCNNNNNNNGG	9 cut(s)	71, 262, 541, 1014, 2916, 3656, 3802, 4362, 4575
AflII	CTTAAG	1 cut(s)	2339
AflIII	ACRYGT	1 cut(s)	2777
AgeI	ACCGGT	1 cut(s)	2490
AhdI	GACNNNNNGTC	1 cut(s)	4932
AjiI	CACGTC	1 cut(s)	85
AjuI	GAANNNNNNNTTGG	6 cut(s)	2223, 2255, 2463, 2495, 3837, 3869
AleI	CACNNNNGTG	2 cut(s)	513, 4857
Alw21I	GWGCWC	3 cut(s)	2313, 2950, 3295
Alw26I	GTCTC	9 cut(s)	613, 1846, 2002, 2054, 2128, 2229, 2768, 3047, 4842
Alw44I	GTGCAC	1 cut(s)	2946
AlwNI	CAGNNNCTG	3 cut(s)	19, 1121, 5195
Ama87I	CYCGRG	1 cut(s)	2032
Aor51HI	AGCGCT	1 cut(s)	4582
ApaI	GGGCCC	1 cut(s)	3969
ApaLI	GTGCAC	1 cut(s)	2946
ApoI	RAATTY	6 cut(s)	128, 1145, 1685, 1851, 2901, 3109
ArsI	GACNNNNNNTTYG	2 cut(s)	4529, 4561
AsiGI	ACCGGT	1 cut(s)	2490
Asp700I	GAANNNNTTC	1 cut(s)	3077
AspLEI	GCGC	4 cut(s)	2176, 4506, 4583, 4825
AsuC2I	CCSGG	1 cut(s)	3928
AsuII	TTCGAA	1 cut(s)	1689
AsuNHI	GCTAGC	1 cut(s)	1864
AvaI	CYCGRG	1 cut(s)	2032
AvaII	GGWCC	7 cut(s)	200, 265, 1295, 1466, 1950, 2270, 4388
AxyI	CCTNAGG	2 cut(s)	1200, 4716
BaeGI	GKGCMC	2 cut(s)	2950, 3969
BalI	TGGCCA	1 cut(s)	3663
BamHI	GGATCC	2 cut(s)	2511, 3454
BanII	GRGCYC	2 cut(s)	3969, 5087
BauI	CACGAG	1 cut(s)	4272
BbrPI	CACGTG	1 cut(s)	1040
BbsI	GAAGAC	5 cut(s)	1769, 2885, 3518, 4469, 5160
Bbv12I	GWGCWC	3 cut(s)	2313, 2950, 3295
BbvCI	CCTCAGC	2 cut(s)	33, 1173
BceAI	ACGGC	4 cut(s)	108, 170, 2027, 2901
BciVI	GTATCC	4 cut(s)	1199, 2683, 3082, 3337
BclI	TGATCA	1 cut(s)	4359
BcnI	CCSGG	1 cut(s)	3928
BcoDI	GTCTC	9 cut(s)	613, 1846, 2002, 2054, 2128, 2229, 2768, 3047, 4842
BfaI	CTAG	7 cut(s)	951, 1368, 1463, 1865, 3977, 4412, 5145
BfmI	CTRYAG	6 cut(s)	642, 1815, 2538, 3396, 3765, 4134
BfoI	RGCGCY	1 cut(s)	4584
BfrI	CTTAAG	1 cut(s)	2339
BfuAI	ACCTGC	6 cut(s)	1306, 2167, 2278, 3328, 3553, 4751
BfuI	GTATCC	4 cut(s)	1199, 2683, 3082, 3337
BlpI	GCTNAGC	1 cut(s)	2349
BmcAI	AGTACT	1 cut(s)	5114
Bme18I	GGWCC	7 cut(s)	200, 265, 1295, 1466, 1950, 2270, 4388
BmeRI	GACNNNNNGTC	1 cut(s)	4932
BmeT110I	CYCGRG	1 cut(s)	2032
BmgBI	CACGTC	1 cut(s)	85
BmrI	ACTGGG	1 cut(s)	4770
BmtI	GCTAGC	1 cut(s)	1868
BmuI	ACTGGG	1 cut(s)	4770
BpiI	GAAGAC	5 cut(s)	1769, 2885, 3518, 4469, 5160
BpmI	CTGGAG	4 cut(s)	3459, 4866, 4950, 5145
Bpu10I	CCTNAGC	3 cut(s)	33, 1173, 3819
Bpu1102I	GCTNAGC	1 cut(s)	2349
Bpu14I	TTCGAA	1 cut(s)	1689
BpuEI	CTTGAG	2 cut(s)	1828, 2348
BpuMI	CCSGG	1 cut(s)	3928
BsaAI	YACGTR	1 cut(s)	1040
BsaHI	GRCGYC	1 cut(s)	2791
BsaI	GGTCTC	3 cut(s)	613, 2128, 4842
BsaWI	WCCGGW	1 cut(s)	2490
Bsc4I	CCNNNNNNNGG	9 cut(s)	71, 262, 541, 1014, 2916, 3656, 3802, 4362, 4575
Bse118I	RCCGGY	2 cut(s)	2490, 3243
Bse21I	CCTNAGG	2 cut(s)	1200, 4716
Bse3DI	GCAATG	8 cut(s)	312, 1749, 2333, 2528, 3402, 3414, 3693, 4131
BseLI	CCNNNNNNNGG	9 cut(s)	71, 262, 541, 1014, 2916, 3656, 3802, 4362, 4575
BseMI	GCAATG	8 cut(s)	312, 1749, 2333, 2528, 3402, 3414, 3693, 4131
BseSI	GKGCMC	2 cut(s)	2950, 3969
BseYI	CCCAGC	4 cut(s)	144, 674, 4550, 4583
BsgI	GTGCAG	4 cut(s)	1614, 1955, 3331, 5193
Bsh1236I	CGCG	3 cut(s)	8, 1988, 3264
Bsh1285I	CGRYCG	1 cut(s)	3289
BshTI	ACCGGT	1 cut(s)	2490
BsiEI	CGRYCG	1 cut(s)	3289
BsiHKAI	GWGCWC	3 cut(s)	2313, 2950, 3295
BsiHKCI	CYCGRG	1 cut(s)	2032
BsiSI	CCGG	7 cut(s)	751, 1165, 2491, 3244, 3927, 4691, 4791
BslFI	GGGAC	2 cut(s)	1181, 5120
BslI	CCNNNNNNNGG	9 cut(s)	71, 262, 541, 1014, 2916, 3656, 3802, 4362, 4575
BsmAI	GTCTC	9 cut(s)	613, 1846, 2002, 2054, 2128, 2229, 2768, 3047, 4842
BsmBI	CGTCTC	1 cut(s)	2002
BsmFI	GGGAC	2 cut(s)	1181, 5120
BsmI	GAATGC	1 cut(s)	849
Bso31I	GGTCTC	3 cut(s)	613, 2128, 4842
BsoBI	CYCGRG	1 cut(s)	2032
Bsp119I	TTCGAA	1 cut(s)	1689
Bsp120I	GGGCCC	1 cut(s)	3965
Bsp1286I	GDGCHC	5 cut(s)	2313, 2950, 3295, 3969, 5087
Bsp1407I	TGTACA	1 cut(s)	741
Bsp1720I	GCTNAGC	1 cut(s)	2349
Bsp19I	CCATGG	1 cut(s)	449
Bsp68I	TCGCGA	1 cut(s)	3264
BspFNI	CGCG	3 cut(s)	8, 1988, 3264
BspHI	TCATGA	1 cut(s)	3388
BspMI	ACCTGC	6 cut(s)	1306, 2167, 2278, 3328, 3553, 4751
BspOI	GCTAGC	1 cut(s)	1868
BspT104I	TTCGAA	1 cut(s)	1689
BspTI	CTTAAG	1 cut(s)	2339
BspTNI	GGTCTC	3 cut(s)	613, 2128, 4842
BsrBI	CCGCTC	1 cut(s)	60
BsrDI	GCAATG	8 cut(s)	312, 1749, 2333, 2528, 3402, 3414, 3693, 4131
BsrFI	RCCGGY	2 cut(s)	2490, 3243
BsrGI	TGTACA	1 cut(s)	741
BssAI	RCCGGY	2 cut(s)	2490, 3243
BssNI	GRCGYC	1 cut(s)	2791
BssSI	CACGAG	1 cut(s)	4272
BssT1I	CCWWGG	4 cut(s)	196, 449, 2409, 4974
Bst2BI	CACGAG	1 cut(s)	4272
Bst6I	CTCTTC	1 cut(s)	5129
BstACI	GRCGYC	1 cut(s)	2791
BstAFI	CTTAAG	1 cut(s)	2339
BstAPI	GCANNNNNTGC	2 cut(s)	3695, 4130
BstAUI	TGTACA	1 cut(s)	741
BstBAI	YACGTR	1 cut(s)	1040
BstBI	TTCGAA	1 cut(s)	1689
BstDSI	CCRYGG	1 cut(s)	449
BstEII	GGTNACC	1 cut(s)	4335
BstENI	CCTNNNNNAGG	2 cut(s)	3654, 3800
BstFNI	CGCG	3 cut(s)	8, 1988, 3264
BstH2I	RGCGCY	1 cut(s)	4584
BstHHI	GCGC	4 cut(s)	2176, 4506, 4583, 4825
BstMAI	GTCTC	9 cut(s)	613, 1846, 2002, 2054, 2128, 2229, 2768, 3047, 4842
BstMCI	CGRYCG	1 cut(s)	3289
BstNSI	RCATGY	5 cut(s)	1341, 1948, 2781, 3959, 4210
BstPI	GGTNACC	1 cut(s)	4335
BstSFI	CTRYAG	6 cut(s)	642, 1815, 2538, 3396, 3765, 4134
BstSLI	GKGCMC	2 cut(s)	2950, 3969
BstUI	CGCG	3 cut(s)	8, 1988, 3264
BstV2I	GAAGAC	5 cut(s)	1769, 2885, 3518, 4469, 5160
BstX2I	RGATCY	7 cut(s)	454, 2511, 2919, 3454, 3772, 4417, 4998
BstXI	CCANNNNNNTGG	3 cut(s)	1709, 3671, 5127
BstYI	RGATCY	7 cut(s)	454, 2511, 2919, 3454, 3772, 4417, 4998
Bsu36I	CCTNAGG	2 cut(s)	1200, 4716
BsuI	GTATCC	4 cut(s)	1199, 2683, 3082, 3337
BtgI	CCRYGG	1 cut(s)	449
BtgZI	GCGATG	3 cut(s)	2118, 2268, 2582
BtrI	CACGTC	1 cut(s)	85
BtsI	GCAGTG	3 cut(s)	799, 2792, 4836
BtuMI	TCGCGA	1 cut(s)	3264
BveI	ACCTGC	6 cut(s)	1306, 2167, 2278, 3328, 3553, 4751
CaiI	CAGNNNCTG	3 cut(s)	19, 1121, 5195
CciI	TCATGA	1 cut(s)	3388
CfoI	GCGC	4 cut(s)	2176, 4506, 4583, 4825
Cfr10I	RCCGGY	2 cut(s)	2490, 3243
CseI	GACGC	1 cut(s)	2780
Csp6I	GTAC	7 cut(s)	742, 1750, 1810, 4105, 4353, 4889, 5113
CspAI	ACCGGT	1 cut(s)	2490
CspCI	CAANNNNNGTGG	2 cut(s)	3285, 3320
CviQI	GTAC	7 cut(s)	742, 1750, 1810, 4105, 4353, 4889, 5113
DraI	TTTAAA	2 cut(s)	589, 4057
DrdI	GACNNNNNNGTC	1 cut(s)	5212
DriI	GACNNNNNGTC	1 cut(s)	4932
DseDI	GACNNNNNNGTC	1 cut(s)	5212
EaeI	YGGCCR	4 cut(s)	3, 55, 1162, 3661
Eam1104I	CTCTTC	1 cut(s)	5129
Eam1105I	GACNNNNNGTC	1 cut(s)	4932
EarI	CTCTTC	1 cut(s)	5129
EciI	GGCGGA	1 cut(s)	1393
Eco130I	CCWWGG	4 cut(s)	196, 449, 2409, 4974
Eco24I	GRGCYC	2 cut(s)	3969, 5087
Eco31I	GGTCTC	3 cut(s)	613, 2128, 4842
Eco32I	GATATC	4 cut(s)	1482, 3895, 3906, 4428
Eco47I	GGWCC	7 cut(s)	200, 265, 1295, 1466, 1950, 2270, 4388
Eco47III	AGCGCT	1 cut(s)	4582
Eco57I	CTGAAG	3 cut(s)	2570, 3555, 3864
Eco72I	CACGTG	1 cut(s)	1040
Eco81I	CCTNAGG	2 cut(s)	1200, 4716
Eco88I	CYCGRG	1 cut(s)	2032
Eco91I	GGTNACC	1 cut(s)	4335
EcoNI	CCTNNNNNAGG	2 cut(s)	3654, 3800
EcoO109I	RGGNCCY	3 cut(s)	598, 1466, 3966
EcoO65I	GGTNACC	1 cut(s)	4335
EcoRI	GAATTC	2 cut(s)	1145, 1685
EcoRV	GATATC	4 cut(s)	1482, 3895, 3906, 4428
EcoT14I	CCWWGG	4 cut(s)	196, 449, 2409, 4974
EcoT22I	ATGCAT	1 cut(s)	2284
EcoT38I	GRGCYC	2 cut(s)	3969, 5087
ErhI	CCWWGG	4 cut(s)	196, 449, 2409, 4974
Esp3I	CGTCTC	1 cut(s)	2002
FalI	AAGNNNNNCTT	6 cut(s)	280, 312, 585, 617, 2310, 2342
FaqI	GGGAC	2 cut(s)	1181, 5120
FauI	CCCGC	1 cut(s)	4160
FauNDI	CATATG	3 cut(s)	746, 1096, 2142
FbaI	TGATCA	1 cut(s)	4359
FblI	GTMKAC	1 cut(s)	1599
FriOI	GRGCYC	2 cut(s)	3969, 5087
FspBI	CTAG	7 cut(s)	951, 1368, 1463, 1865, 3977, 4412, 5145
FspI	TGCGCA	2 cut(s)	2175, 4824
GlaI	GCGC	4 cut(s)	2175, 4505, 4582, 4824
GsaI	CCCAGC	4 cut(s)	148, 678, 4554, 4587
GsuI	CTGGAG	4 cut(s)	3459, 4866, 4950, 5145
HaeII	RGCGCY	1 cut(s)	4584
HapII	CCGG	7 cut(s)	751, 1165, 2491, 3244, 3927, 4691, 4791
HgaI	GACGC	1 cut(s)	2780
HhaI	GCGC	4 cut(s)	2176, 4506, 4583, 4825
Hin1I	GRCGYC	1 cut(s)	2791
Hin6I	GCGC	4 cut(s)	2174, 4504, 4581, 4823
HinP1I	GCGC	4 cut(s)	2174, 4504, 4581, 4823
HincII	GTYRAC	2 cut(s)	1489, 2236
HindII	GTYRAC	2 cut(s)	1489, 2236
HindIII	AAGCTT	3 cut(s)	160, 2429, 2550
HpaII	CCGG	7 cut(s)	751, 1165, 2491, 3244, 3927, 4691, 4791
Hpy166II	GTNNAC	7 cut(s)	835, 1489, 1600, 1966, 2236, 2948, 3808
Hpy8I	GTNNAC	7 cut(s)	835, 1489, 1600, 1966, 2236, 2948, 3808
Hpy99I	CGWCG	3 cut(s)	70, 89, 98
HpyCH4IV	ACGT	4 cut(s)	84, 1039, 1392, 3577
HpySE526I	ACGT	4 cut(s)	84, 1039, 1392, 3577
Hsp92I	GRCGYC	1 cut(s)	2791
HspAI	GCGC	4 cut(s)	2174, 4504, 4581, 4823
Ksp22I	TGATCA	1 cut(s)	4359
MaeI	CTAG	7 cut(s)	951, 1368, 1463, 1865, 3977, 4412, 5145
MaeII	ACGT	4 cut(s)	84, 1039, 1392, 3577
MbiI	CCGCTC	1 cut(s)	60
MfeI	CAATTG	4 cut(s)	466, 2471, 3782, 4437
MflI	RGATCY	7 cut(s)	454, 2511, 2919, 3454, 3772, 4417, 4998
MhlI	GDGCHC	5 cut(s)	2313, 2950, 3295, 3969, 5087
MlsI	TGGCCA	1 cut(s)	3663
MluNI	TGGCCA	1 cut(s)	3663
MmeI	TCCRAC	8 cut(s)	286, 499, 1244, 1595, 1598, 2248, 3617, 4615
Mox20I	TGGCCA	1 cut(s)	3663
Mph1103I	ATGCAT	1 cut(s)	2284
MroXI	GAANNNNTTC	1 cut(s)	3077
MscI	TGGCCA	1 cut(s)	3663
MslI	CAYNNNNRTG	7 cut(s)	513, 1063, 1074, 3407, 3918, 3960, 4857
Msp20I	TGGCCA	1 cut(s)	3663
MspA1I	CMGCKG	5 cut(s)	37, 3619, 4558, 5134, 5192
MspCI	CTTAAG	1 cut(s)	2339
MspI	CCGG	7 cut(s)	751, 1165, 2491, 3244, 3927, 4691, 4791
MunI	CAATTG	4 cut(s)	466, 2471, 3782, 4437
Mva1269I	GAATGC	1 cut(s)	849
MvnI	CGCG	3 cut(s)	8, 1988, 3264
NciI	CCSGG	1 cut(s)	3928
NcoI	CCATGG	1 cut(s)	449
NdeI	CATATG	3 cut(s)	746, 1096, 2142
NheI	GCTAGC	1 cut(s)	1864
NmuCI	GTSAC	9 cut(s)	15, 890, 986, 1056, 3360, 3586, 4535, 4684, 5203
NruI	TCGCGA	1 cut(s)	3264
NsbI	TGCGCA	2 cut(s)	2175, 4824
NsiI	ATGCAT	1 cut(s)	2284
NspI	RCATGY	5 cut(s)	1341, 1948, 2781, 3959, 4210
NspV	TTCGAA	1 cut(s)	1689
OliI	CACNNNNGTG	2 cut(s)	513, 4857
PaeI	GCATGC	1 cut(s)	3959
PaeR7I	CTCGAG	1 cut(s)	2032
PagI	TCATGA	1 cut(s)	3388
PasI	CCCWGGG	1 cut(s)	3969
PciI	ACATGT	1 cut(s)	2777
PcsI	WCGNNNNNNNCGW	2 cut(s)	90, 2788
PctI	GAATGC	1 cut(s)	849
PdmI	GAANNNNTTC	1 cut(s)	3077
PfeI	GAWTC	8 cut(s)	309, 1126, 1189, 1436, 2561, 3342, 4366, 4961
PflFI	GACNNNGTC	1 cut(s)	3058
PfoI	TCCNGGA	1 cut(s)	1468
PinAI	ACCGGT	1 cut(s)	2490
Ple19I	CGATCG	1 cut(s)	3289
PmaCI	CACGTG	1 cut(s)	1040
PmlI	CACGTG	1 cut(s)	1040
Ppu21I	YACGTR	1 cut(s)	1040
PpuMI	RGGWCCY	1 cut(s)	1466
PscI	ACATGT	1 cut(s)	2777
PsiI	TTATAA	1 cut(s)	900
Psp1406I	AACGTT	1 cut(s)	1392
Psp5II	RGGWCCY	1 cut(s)	1466
PspCI	CACGTG	1 cut(s)	1040
PspEI	GGTNACC	1 cut(s)	4335
PspFI	CCCAGC	4 cut(s)	144, 674, 4550, 4583
PspOMI	GGGCCC	1 cut(s)	3965
PspPPI	RGGWCCY	1 cut(s)	1466
PspXI	VCTCGAGB	1 cut(s)	2032
PsrI	GAACNNNNNNTAC	2 cut(s)	3318, 3350
PstNI	CAGNNNCTG	3 cut(s)	19, 1121, 5195
PsuI	RGATCY	7 cut(s)	454, 2511, 2919, 3454, 3772, 4417, 4998
PsyI	GACNNNGTC	1 cut(s)	3058
PvuI	CGATCG	1 cut(s)	3289
PvuII	CAGCTG	5 cut(s)	37, 3619, 4558, 5134, 5192
RruI	TCGCGA	1 cut(s)	3264
RsaI	GTAC	7 cut(s)	743, 1751, 1811, 4106, 4354, 4890, 5114
RsaNI	GTAC	7 cut(s)	742, 1750, 1810, 4105, 4353, 4889, 5113
RseI	CAYNNNNRTG	7 cut(s)	513, 1063, 1074, 3407, 3918, 3960, 4857
ScaI	AGTACT	1 cut(s)	5114
SduI	GDGCHC	5 cut(s)	2313, 2950, 3295, 3969, 5087
SfcI	CTRYAG	6 cut(s)	642, 1815, 2538, 3396, 3765, 4134
Sfr274I	CTCGAG	1 cut(s)	2032
SfuI	TTCGAA	1 cut(s)	1689
SinI	GGWCC	7 cut(s)	200, 265, 1295, 1466, 1950, 2270, 4388
SlaI	CTCGAG	1 cut(s)	2032
SmiMI	CAYNNNNRTG	7 cut(s)	513, 1063, 1074, 3407, 3918, 3960, 4857
SmlI	CTYRAG	4 cut(s)	1843, 2032, 2327, 2339
SmoI	CTYRAG	4 cut(s)	1843, 2032, 2327, 2339
SphI	GCATGC	1 cut(s)	3959
SspI	AATATT	3 cut(s)	583, 2257, 4624
SspMI	CTAG	7 cut(s)	951, 1368, 1463, 1865, 3977, 4412, 5145
StyI	CCWWGG	4 cut(s)	196, 449, 2409, 4974
TaiI	ACGT	4 cut(s)	87, 1042, 1395, 3580
TatI	WGTACW	3 cut(s)	741, 4888, 5112
TauI	GCSGC	3 cut(s)	8, 60, 1142
TfiI	GAWTC	8 cut(s)	309, 1126, 1189, 1436, 2561, 3342, 4366, 4961
TseFI	GTSAC	9 cut(s)	15, 890, 986, 1056, 3360, 3586, 4535, 4684, 5203
Tsp45I	GTSAC	9 cut(s)	15, 890, 986, 1056, 3360, 3586, 4535, 4684, 5203
TspGWI	ACGGA	4 cut(s)	150, 169, 1008, 4267
Tth111I	GACNNNGTC	1 cut(s)	3058
Vha464I	CTTAAG	1 cut(s)	2339
VneI	GTGCAC	1 cut(s)	2946
VpaK11BI	GGWCC	7 cut(s)	200, 265, 1295, 1466, 1950, 2270, 4388
XagI	CCTNNNNNAGG	2 cut(s)	3654, 3800
XapI	RAATTY	6 cut(s)	128, 1145, 1685, 1851, 2901, 3109
XceI	RCATGY	5 cut(s)	1341, 1948, 2781, 3959, 4210
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1709
XhoI	CTCGAG	1 cut(s)	2032
XmiI	GTMKAC	1 cut(s)	1599
XmnI	GAANNNNTTC	1 cut(s)	3077
XspI	CTAG	7 cut(s)	951, 1368, 1463, 1865, 3977, 4412, 5145
ZrmI	AGTACT	1 cut(s)	5114
Zsp2I	ATGCAT	1 cut(s)	2284

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.