Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0005346

Physical Location & Seq

Genomic Coordinates Reverse (-)

38314663 .. 38340217

Gene Structure 25555 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G025530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4851 bp

ATGGCGGTTTTGCAGTCACTGGCTCCTGTTCCTCAGCTGCTTCACTCCTCCAATGGCCGCTCCCCGACGAGAGGACTCTTTCTCGTCGATTTCGTCGGCTACTGCAAATCAAAGAGGACTCGCCGTAAATTCAACGGAGCATCCCAGCCTCTACGGACAAGCTTCCCTCAATTCGTTTCCAAAGCCGTGCTCGACCTTGGACCCTCCTCTACCGATCACTCAACTCCTCCTCCTCCTACTACTACTTCCAAACCCCAGGTTGCGGACCTGAAAGATATAATATCAGAAAGAGGAGCTTGCGGAGTTGGATTCATTGCCAATCTGGACAACAAAGCATCTCATGGCATTGTTAAGGATGCTCTTACAGCTCTTGGCTGCATGGAACATCGTGGAGGCTGTGGAGCAGATAATGACTCTGGTGATGGTTCAGGATTGATGAGTTCAATCCCATGGGATCTATTTAACAATTGGGCTGACAAGCAAGGGATTGCATCATTTGACAAGTTACACACTGGTGTTGGAATGATTTTCCTCCCCAAAGAGGACAACCTTATGAAAGAAGCCAAAAAAGCTATTGTGAATATTTTTAAACAAGAGGGTCTTGAAGTGCTTGGGTGGAGACCTGTTCCAGTGAATACAGCTATAGTTGGTTTCTATGCAAAAGAAACAATGCCCAGCATACAACAGGTATTCGTCAAAGTTGTTAAAGAGGAAAAAGTTGAGGACATTGAGCGAGAACTGTACATATGCCGGAAGTTAATCGAAAGAGCAGCAAGCTCAGAAAGTTGGGGCAGTGATCTTTATTTCTGTTCTTTGTCCAATCAAACAATAGTTTACAAAGGAATGCTACGCTCAGAAGTTCTTGGTTTATTTTATTCTGACCTCCAAAGTGACCTTTATAAATCCGCTTTTGCCATCTATCATCGAAGGTATAGCACAAATACCACTCCTAGATGGCCCCTTGCACAACCAATGAGGTTACTTGGTCACAATGGAGAGATCAACACCATACAGGGGAACTTGAACTGGATGCAATCACGTGAAGCCTCACTGAAGTCACCTGTATGGCATGATCGTGAAAATGAACTTCGTCCATATGGCAATCCAAAGGGTTCAGACTCTGCAAATCTGGACAGTGCGGCTGAATTCTTACTGAGAAGTGGCCGGACTGCTGAGGAGGCTCTGATGATTCTTGTCCCTGAGGGATACAAAAATCATCCAACTCTGATGATTAACTATCCAGAGGTGGTTGATTTTTATGACTATTACAAGGGTCAGATGGAGGCTTGGGATGGACCTGCTTTATTGTTATTCAGTGATGGAAAAACAGTTGGGGCATGTCTTGATCGTAATGGACTTCGGCCTGCTAGATATTGGCGGACATCTGATAACGTTGTTTATGTCGCATCTGAGGTTGGGGTTCTTCCAGTGGATGATTCAAAAGTTACAATGAAAGGTCGTCTAGGTCCTGGAATGATGATATCTGTTGACTTATTAAGTGGTCAGGTTTATGAGAACACAGAAGTTAAGAAGCGAGTGGCTTCGTCAAATCCATATGGAAAGTGGGTGCAGGAAAACATGCGGACCTTAAAGACTGTGAACTTCCTTTCATCCACAATCGCGGACAATGATGCAATACTGAGACGACAACAGGCATTTGGTTACTCGAGTGAGGATGTCCAAATGGTTATCGAGACAATGGCTTCACAAGGGAAGGAGCCTACATTTTGCATGGGCGATGATATTCCATTGGCGATTTTATCTCAGAGACCACATATGCTATATGACTATTTCAAGCAACGGTTTGCGCAGGTTACAAATCCAGCCATTGATCCTCTACGAGAAGGGTTAGTTATGTCTCTTGAAGTCAACATTGGAAAGCGACAAAATATTTTGGAGGTTGGACCTGAAAATGCATCACAGGTTATCTTGTCCAGTCCTGTGCTCAATGAAGGAGAACTTGAGTCATTGCTTAAGGATGCTCAGCTAAAACCCCAAGTTTTACCTACATTTTTTGATATTCATAAAGGGGTTGATGGTTCCTTGGAAAAAACGCTGTATAAGCTTTGTGAAGCTGCTGATGATGCTGTTCAAAATGGTTGCCAATTGCTTGTTCTGTCTGACCGGTCTGATGAGTTGGAAGCAACTCGTCCTGCCATTCCAATCCTTCTTGCTGTTGGTGCTGTTCATCAGCATCTTATTCAAAATGGGTTGAGAATGTCAGCTTCTATAATAGTGGATACTGCTCAGTGCTTTAGCACTCATCAATTTGCCTGTTTGATTGGATATGGTGCAAGTGCTGTGTGTCCATACTTGGCATTGGAGACATGTCGGCAGTGGCGTCTGAGTAATAAAACAGTAAATCTAATGCGGAACGGGAAGATGCCTACTGTAACGATTGAACAGGCTCAAAAGAACTTCTGCAAGGCTGTTACGGCTGGTCTTCTTAAAATTCTTTCCAAAATGGGGATCTCGTTGCTTTCAAGTTATTGCGGTGCACAGATTTTTGAGATTTATGGATTGGGGAAGGAGGTTGTTGATCTTGCATTTTGTGGGAGTATATCAAGTATTGGTGGATTAACTTTTGATGAGCTGGCGAGGGAGACTTTGTCCTTCTGGGTGAAGGCTTTCTCTGAGGATACAGCTAAACGACTGGAAAATTTTGGATTTATACAATTCAGACCAGGAGGGGAGTATCATGGAAACAACCCAGAGATGTCGAAATTGCTACACAAAGCTGTTCGACAGAAGAATGAAAGTGCCTTTTCTGTTTATCAGCAGCATTTAGCTAACCGGCCCGTTAATGTTCTTCGCGATCTCATTGAGTTCAAAAGCGATCGTTCTCCAATCCCTGTGGGAAAGGTTGAACCTGCTGTATCCATTGTTCAACGATTCTGCACTGGTGGGATGTCACTTGGAGCTATTTCCAGAGAAACTCATGAGGCTATAGCCATTGCAATGAATAGAATAGGTGGAAAGTCCAATTCTGGAGAAGGTGGTGAGGATCCTATTCGCTGGAAACCACTTGCAGATGTTGTTGATGGGTATTCACCGACCCTTCCACATCTAAAAGGTCTTCAAAATGGAGATACTGCTACAAGTGCTATCAAGCAGGTTGCTTCAGGACGTTTTGGAGTCACTCCAACCTTCTTGGTCAATGCCGATCAGCTGGAAATCAAAATTGCACAAGGGGCGAAGCCTGGTGAAGGTGGCCAACTGCCTGGTAAAAAAGTTAGTGCATACATTGCGAGGTTGAGAAACTCGAAACCTGGGGTTCCACTTATATCTCCACCCCCACACCATGACATTTATTCTATAGAGGATCTTGCCCAATTGATCTATGACCTTCATCAGGTGAACCCTAAAGCTAAGGTGTCGGTGAAACTTGTGGCTGAAGCTGGAATTGGCACTGTTGCTTCTGGGGTTGCAAAGGGTAATGCTGATATCATACAGATATCAGGGCATGATGGTGGAACTGGGGCCAGTCCTATAAGTTCCATTAAGCATGCTGGTGGGCCCTGGGAACTAGGACTCACAGAAACACACCAGACACTCATAGAAAATGGACTTAGAGAACGAGTCATTCTTAGAGTTGATGGTGGCTTTAAAAGCGGAGTTGATGTCCTAATGGCTGCAACTATGGGTGCTGATGAGTACGGGTTTGGGTCTGTGGCAATGATTGCTACAGGTTGTGTTATGGCCCGCATTTGCCATACGAATAATTGTCCTGTTGGTGTAGCCAGCCAGAGAGAGGAACTGCGTGCTCGATTTCCTGGCGTGCCTGGTGATCTAGTCAACTTTTTTTTGTATGTTGCCGAGGAGGTAAGAGGCATGTTGGCACAGTTGGGATATGAGAAGTTAGACGATATAATTGGACGGACAGATTTGTTTAGACCACGAGATATATCTCTAATGAAAACACAGCATCTTGATCTCGGTTATATTCTATCAAATGTCGGGTTACCAAAATGGAGCAGTACCATGATCAGGAATCAGGATGTTCATACTAACGGTCCTGTTCTGGATGATATTTTACTAGGAGATCCAGAGATATCAGATGCAATTGAGAATGAAAAAGTGGTTCAGAAGACCGTAAAGATATACAATGTGGATCGTGCTGTTTGTGGGCGTATAGCAGGGGTGGTTGCCAAAAAGTACGGTGACACTGGTTTTGCTGGGCAGCTGAACATAACCTTTACAGGGAGCGCTGGGCAATCATTTGGTTGCTTCTTGACTCCTGGGATGAATATTCGACTTGTTGGAGAGGCAAATGATTATGTGGGAAAGAGTATTTCGGGGGGAGAATTGGTTGTCACTCCGGCAGAGAATACGGGATTTTGCCCTGAGGATGCAACTATTGTAGGGAATACCTGCTTGTATGGTGCAACTGGGGGTCAAATCTTTGTTAGAGGGAAAGCCGGAGAGCGATTTGCTGTAAGAAACTCTCTTGCGCAAGCAGTGGTTGAAGGCACTGGAGACCACTGTTGTGAATACATGACTGGTGGCTGTGTAGTTGTACTTGGAAAAGTGGGTAGAAATGTTGCTGCTGGAATGACTGGAGGTCTGGCATACTTTCTTGATGAGGACGATTCCTTCATCCCCAAGGTAAATAGGGAGATTGTGAAGATCCAGAGAGTCAATGCTCCTGTGGGGCAGATGCAGCTAAAGAGCCTTATTGAAGCCCATGTTGAAAAAACTGGAAGTGGCAAGGGCTCTGTTATTCTGAAGGAGTGGGACAAGTACTTGCCACTCTTCTGGCAGCTGGTTCCCCCTAGTGAAGAAGACACTCCAGAGGCGTGTGCAGACTATGTGAGAACAGCTGCTGGTGAAGTGACTTTGCAGTCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009853 GO:0009941 GO:0009987 GO:0010033 GO:0015930 GO:0016020 GO:0016041 GO:0016053 GO:0016491 GO:0016638 GO:0016643 GO:0019222 GO:0019676 GO:0019740 GO:0019752 GO:0019899 GO:0031323 GO:0031967 GO:0031974 GO:0031975 GO:0034285 GO:0042221 GO:0043085 GO:0043094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0043650 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048046 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051338 GO:0051347 GO:0055114 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0080114 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00630 ko00910 ko01120 map00630 map00910 map01120

Pfam Domains

Protein Families

GATase_2 GXGXG Glu_syn_central Glu_synthase

Protein Analysis

1616

Amino Acids

175.93

Weight (kDa)

6.21

Isoelectric Point (pI)

35.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	100 - 522	1.2e-177	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	548 - 841	1.7e-107	Glutamate synthase central domain
Glu_synthase	PF01645	901 - 1284	2.5e-155	Conserved region in glutamate synthase
GXGXG	PF01493	1366 - 1549	1.7e-78	GXGXG motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005346)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41220 AT5G04140 AT5G04140
fragaria_vesca	FvH4_6g25460 FvH4_6g25460 FvH4_6g25460 FvH4_6g25460
malus_domestica	MD14G1087500.v1.1
prunus_persica	Prupe.7G039100_v2.0.a1
pyrus_communis	pycom14g07760 pycom14g07770
rosa_chinensis	RchiOBHm_Chr2g0125471
rosa_laevigata	RLG00000018438 RLG00000018867 RLG00000030615 RLG00000034746
rosa_multiflora	Rmu_sc0005339.1_g000009 Rmu_sc0005339.1_g000011 Rmu_ssc0000126.1_g000014
rosa_roxburghii	Rroxscaffold_1G00035740 Rroxscaffold_2G00117960
rosa_rugosa	Rorug02G0257500 Rorug02G0257600
rosa_samantha	Rh2AG316500 Rh2BG324000 Rh2BG355400 Rh2CG306200 Rh2DG342600
rosa_wichuraiana	Rw2G025530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	900
AasI	GACNNNNNNGTC	1 cut(s)	4840
Acc16I	TGCGCA	2 cut(s)	1809, 4452
Acc36I	ACCTGC	5 cut(s)	1306, 1801, 2878, 3103, 4379
AccBSI	CCGCTC	1 cut(s)	60
AccII	CGCG	2 cut(s)	1622, 2814
AclI	AACGTT	1 cut(s)	1392
AclWI	GGATC	9 cut(s)	462, 1826, 2477, 2999, 3012, 3330, 4037, 4119, 4621
AcoI	YGGCCR	3 cut(s)	55, 1162, 3211
AcsI	RAATTY	4 cut(s)	128, 1145, 2451, 2659
AcuI	CTGAAG	4 cut(s)	1073, 3105, 3414, 4745
AcvI	CACGTG	1 cut(s)	1040
AcyI	GRCGYC	1 cut(s)	2341
AfaI	GTAC	6 cut(s)	743, 3656, 3979, 4157, 4518, 4742
AfeI	AGCGCT	1 cut(s)	4207
AflII	CTTAAG	1 cut(s)	1973
AflIII	ACRYGT	1 cut(s)	2327
AgeI	ACCGGT	1 cut(s)	2124
AhdI	GACNNNNNGTC	1 cut(s)	4560
AjuI	GAANNNNNNNTTGG	6 cut(s)	1857, 1889, 2097, 2129, 3387, 3419
AleI	CACNNNNGTG	2 cut(s)	513, 4485
Alw21I	GWGCWC	4 cut(s)	192, 1947, 2500, 3766
Alw26I	GTCTC	8 cut(s)	613, 1636, 1688, 1762, 1863, 2318, 2597, 4470
Alw44I	GTGCAC	1 cut(s)	2496
AlwI	GGATC	9 cut(s)	462, 1826, 2477, 2999, 3012, 3330, 4037, 4119, 4621
AlwNI	CAGNNNCTG	3 cut(s)	19, 1121, 4823
Ama87I	CYCGRG	1 cut(s)	1666
Aor51HI	AGCGCT	1 cut(s)	4207
AoxI	GGCC	9 cut(s)	55, 956, 1162, 1361, 2795, 3211, 3480, 3515, 3699
ApaI	GGGCCC	1 cut(s)	3519
ApaLI	GTGCAC	1 cut(s)	2496
ApoI	RAATTY	4 cut(s)	128, 1145, 2451, 2659
ArsI	GACNNNNNNTTYG	2 cut(s)	4154, 4186
AsiGI	ACCGGT	1 cut(s)	2124
Asp700I	GAANNNNTTC	1 cut(s)	2627
AspLEI	GCGC	3 cut(s)	1810, 4208, 4453
AvaI	CYCGRG	1 cut(s)	1666
AvaII	GGWCC	7 cut(s)	200, 265, 1295, 1466, 1584, 1904, 4013
AxyI	CCTNAGG	2 cut(s)	1200, 4344
BaeGI	GKGCMC	2 cut(s)	2500, 3519
BalI	TGGCCA	1 cut(s)	3213
BamHI	GGATCC	1 cut(s)	3004
BanII	GRGCYC	2 cut(s)	3519, 4715
BauI	CACGAG	1 cut(s)	3897
BbrPI	CACGTG	1 cut(s)	1040
BbsI	GAAGAC	4 cut(s)	2435, 3068, 4094, 4788
Bbv12I	GWGCWC	4 cut(s)	192, 1947, 2500, 3766
BbvCI	CCTCAGC	2 cut(s)	33, 1173
BceAI	ACGGC	3 cut(s)	108, 170, 2451
BciVI	GTATCC	4 cut(s)	1199, 2233, 2632, 2887
BclI	TGATCA	1 cut(s)	3984
BcoDI	GTCTC	8 cut(s)	613, 1636, 1688, 1762, 1863, 2318, 2597, 4470
BfaI	CTAG	7 cut(s)	951, 1368, 1463, 3527, 3791, 4037, 4773
BfmI	CTRYAG	4 cut(s)	642, 2946, 3315, 3684
BfoI	RGCGCY	1 cut(s)	4209
BfrI	CTTAAG	1 cut(s)	1973
BfuAI	ACCTGC	5 cut(s)	1306, 1801, 2878, 3103, 4379
BfuI	GTATCC	4 cut(s)	1199, 2233, 2632, 2887
BlpI	GCTNAGC	1 cut(s)	1983
BmcAI	AGTACT	1 cut(s)	4742
Bme18I	GGWCC	7 cut(s)	200, 265, 1295, 1466, 1584, 1904, 4013
BmeRI	GACNNNNNGTC	1 cut(s)	4560
BmeT110I	CYCGRG	1 cut(s)	1666
BmrI	ACTGGG	2 cut(s)	3486, 4398
BmuI	ACTGGG	2 cut(s)	3486, 4398
BpiI	GAAGAC	4 cut(s)	2435, 3068, 4094, 4788
BpmI	CTGGAG	4 cut(s)	3009, 4494, 4578, 4773
Bpu10I	CCTNAGC	3 cut(s)	33, 1173, 3369
Bpu1102I	GCTNAGC	1 cut(s)	1983
BpuEI	CTTGAG	1 cut(s)	1982
BsaAI	YACGTR	1 cut(s)	1040
BsaHI	GRCGYC	1 cut(s)	2341
BsaI	GGTCTC	3 cut(s)	613, 1762, 4470
BsaWI	WCCGGW	1 cut(s)	2124
BsaXI	ACNNNNNCTCC	8 cut(s)	214, 244, 2594, 2624, 3606, 3636, 4293, 4323
Bse118I	RCCGGY	2 cut(s)	2124, 2793
Bse21I	CCTNAGG	2 cut(s)	1200, 4344
Bse3DI	GCAATG	6 cut(s)	312, 1967, 2952, 2964, 3243, 3681
BseMI	GCAATG	6 cut(s)	312, 1967, 2952, 2964, 3243, 3681
BseRI	GAGGAG	8 cut(s)	37, 196, 216, 219, 222, 306, 1190, 3833
BseSI	GKGCMC	2 cut(s)	2500, 3519
BseYI	CCCAGC	4 cut(s)	144, 674, 4175, 4208
BsgI	GTGCAG	3 cut(s)	1589, 2881, 4821
Bsh1236I	CGCG	2 cut(s)	1622, 2814
Bsh1285I	CGRYCG	1 cut(s)	2839
BshFI	GGCC	9 cut(s)	57, 958, 1164, 1363, 2797, 3213, 3482, 3517, 3701
BshTI	ACCGGT	1 cut(s)	2124
BsiEI	CGRYCG	1 cut(s)	2839
BsiHKAI	GWGCWC	4 cut(s)	192, 1947, 2500, 3766
BsiHKCI	CYCGRG	1 cut(s)	1666
BsiSI	CCGG	6 cut(s)	751, 1165, 2125, 2794, 4319, 4419
BslFI	GGGAC	2 cut(s)	1181, 4748
BsmAI	GTCTC	8 cut(s)	613, 1636, 1688, 1762, 1863, 2318, 2597, 4470
BsmBI	CGTCTC	1 cut(s)	1636
BsmFI	GGGAC	2 cut(s)	1181, 4748
BsmI	GAATGC	1 cut(s)	849
BsnI	GGCC	9 cut(s)	57, 958, 1164, 1363, 2797, 3213, 3482, 3517, 3701
Bso31I	GGTCTC	3 cut(s)	613, 1762, 4470
BsoBI	CYCGRG	1 cut(s)	1666
Bsp120I	GGGCCC	1 cut(s)	3515
Bsp1286I	GDGCHC	6 cut(s)	192, 1947, 2500, 3519, 3766, 4715
Bsp1407I	TGTACA	1 cut(s)	741
Bsp1720I	GCTNAGC	1 cut(s)	1983
Bsp19I	CCATGG	1 cut(s)	449
Bsp68I	TCGCGA	1 cut(s)	2814
BspANI	GGCC	9 cut(s)	57, 958, 1164, 1363, 2797, 3213, 3482, 3517, 3701
BspFNI	CGCG	2 cut(s)	1622, 2814
BspHI	TCATGA	1 cut(s)	2938
BspMI	ACCTGC	5 cut(s)	1306, 1801, 2878, 3103, 4379
BspPI	GGATC	9 cut(s)	462, 1826, 2477, 2999, 3012, 3330, 4037, 4119, 4621
BspTI	CTTAAG	1 cut(s)	1973
BspTNI	GGTCTC	3 cut(s)	613, 1762, 4470
BsrBI	CCGCTC	1 cut(s)	60
BsrDI	GCAATG	6 cut(s)	312, 1967, 2952, 2964, 3243, 3681
BsrFI	RCCGGY	2 cut(s)	2124, 2793
BsrGI	TGTACA	1 cut(s)	741
BssAI	RCCGGY	2 cut(s)	2124, 2793
BssNI	GRCGYC	1 cut(s)	2341
BssSI	CACGAG	1 cut(s)	3897
BssT1I	CCWWGG	4 cut(s)	196, 449, 2043, 4602
Bst2BI	CACGAG	1 cut(s)	3897
Bst6I	CTCTTC	1 cut(s)	4757
BstACI	GRCGYC	1 cut(s)	2341
BstAFI	CTTAAG	1 cut(s)	1973
BstAPI	GCANNNNNTGC	2 cut(s)	3245, 3680
BstAUI	TGTACA	1 cut(s)	741
BstBAI	YACGTR	1 cut(s)	1040
BstDSI	CCRYGG	1 cut(s)	449
BstEII	GGTNACC	1 cut(s)	3960
BstENI	CCTNNNNNAGG	2 cut(s)	3204, 3350
BstFNI	CGCG	2 cut(s)	1622, 2814
BstH2I	RGCGCY	1 cut(s)	4209
BstHHI	GCGC	3 cut(s)	1810, 4208, 4453
BstMAI	GTCTC	8 cut(s)	613, 1636, 1688, 1762, 1863, 2318, 2597, 4470
BstMCI	CGRYCG	1 cut(s)	2839
BstNSI	RCATGY	5 cut(s)	1341, 1582, 2331, 3509, 3835
BstPI	GGTNACC	1 cut(s)	3960
BstSFI	CTRYAG	4 cut(s)	642, 2946, 3315, 3684
BstSLI	GKGCMC	2 cut(s)	2500, 3519
BstUI	CGCG	2 cut(s)	1622, 2814
BstV2I	GAAGAC	4 cut(s)	2435, 3068, 4094, 4788
BstX2I	RGATCY	6 cut(s)	454, 2469, 3004, 3322, 4042, 4626
BstXI	CCANNNNNNTGG	2 cut(s)	3221, 4755
BstYI	RGATCY	6 cut(s)	454, 2469, 3004, 3322, 4042, 4626
Bsu36I	CCTNAGG	2 cut(s)	1200, 4344
BsuI	GTATCC	4 cut(s)	1199, 2233, 2632, 2887
BsuRI	GGCC	9 cut(s)	57, 958, 1164, 1363, 2797, 3213, 3482, 3517, 3701
BtgI	CCRYGG	1 cut(s)	449
BtgZI	GCGATG	1 cut(s)	1752
BtsI	GCAGTG	3 cut(s)	799, 2342, 4464
BtuMI	TCGCGA	1 cut(s)	2814
BveI	ACCTGC	5 cut(s)	1306, 1801, 2878, 3103, 4379
CaiI	CAGNNNCTG	3 cut(s)	19, 1121, 4823
CciI	TCATGA	1 cut(s)	2938
CfoI	GCGC	3 cut(s)	1810, 4208, 4453
Cfr10I	RCCGGY	2 cut(s)	2124, 2793
CseI	GACGC	1 cut(s)	2330
Csp6I	GTAC	6 cut(s)	742, 3655, 3978, 4156, 4517, 4741
CspAI	ACCGGT	1 cut(s)	2124
CspCI	CAANNNNNGTGG	2 cut(s)	2835, 2870
CviQI	GTAC	6 cut(s)	742, 3655, 3978, 4156, 4517, 4741
DraI	TTTAAA	2 cut(s)	589, 3607
DrdI	GACNNNNNNGTC	1 cut(s)	4840
DriI	GACNNNNNGTC	1 cut(s)	4560
DseDI	GACNNNNNNGTC	1 cut(s)	4840
EaeI	YGGCCR	3 cut(s)	55, 1162, 3211
Eam1104I	CTCTTC	1 cut(s)	4757
Eam1105I	GACNNNNNGTC	1 cut(s)	4560
EarI	CTCTTC	1 cut(s)	4757
EciI	GGCGGA	1 cut(s)	1393
Eco130I	CCWWGG	4 cut(s)	196, 449, 2043, 4602
Eco24I	GRGCYC	2 cut(s)	3519, 4715
Eco31I	GGTCTC	3 cut(s)	613, 1762, 4470
Eco32I	GATATC	4 cut(s)	1482, 3445, 3456, 4053
Eco47I	GGWCC	7 cut(s)	200, 265, 1295, 1466, 1584, 1904, 4013
Eco47III	AGCGCT	1 cut(s)	4207
Eco57I	CTGAAG	4 cut(s)	1073, 3105, 3414, 4745
Eco72I	CACGTG	1 cut(s)	1040
Eco81I	CCTNAGG	2 cut(s)	1200, 4344
Eco88I	CYCGRG	1 cut(s)	1666
Eco91I	GGTNACC	1 cut(s)	3960
EcoNI	CCTNNNNNAGG	2 cut(s)	3204, 3350
EcoO109I	RGGNCCY	2 cut(s)	1466, 3516
EcoO65I	GGTNACC	1 cut(s)	3960
EcoRI	GAATTC	1 cut(s)	1145
EcoRV	GATATC	4 cut(s)	1482, 3445, 3456, 4053
EcoT14I	CCWWGG	4 cut(s)	196, 449, 2043, 4602
EcoT22I	ATGCAT	1 cut(s)	1918
EcoT38I	GRGCYC	2 cut(s)	3519, 4715
ErhI	CCWWGG	4 cut(s)	196, 449, 2043, 4602
Esp3I	CGTCTC	1 cut(s)	1636
FalI	AAGNNNNNCTT	6 cut(s)	280, 312, 585, 617, 1944, 1976
FaqI	GGGAC	2 cut(s)	1181, 4748
FauI	CCCGC	1 cut(s)	3710
FauNDI	CATATG	4 cut(s)	746, 1096, 1555, 1776
FbaI	TGATCA	1 cut(s)	3984
FriOI	GRGCYC	2 cut(s)	3519, 4715
FspBI	CTAG	7 cut(s)	951, 1368, 1463, 3527, 3791, 4037, 4773
FspI	TGCGCA	2 cut(s)	1809, 4452
GlaI	GCGC	3 cut(s)	1809, 4207, 4452
GsaI	CCCAGC	4 cut(s)	148, 678, 4179, 4212
GsuI	CTGGAG	4 cut(s)	3009, 4494, 4578, 4773
HaeII	RGCGCY	1 cut(s)	4209
HaeIII	GGCC	9 cut(s)	57, 958, 1164, 1363, 2797, 3213, 3482, 3517, 3701
HapII	CCGG	6 cut(s)	751, 1165, 2125, 2794, 4319, 4419
HgaI	GACGC	1 cut(s)	2330
HhaI	GCGC	3 cut(s)	1810, 4208, 4453
Hin1I	GRCGYC	1 cut(s)	2341
Hin6I	GCGC	3 cut(s)	1808, 4206, 4451
HinP1I	GCGC	3 cut(s)	1808, 4206, 4451
HincII	GTYRAC	3 cut(s)	1489, 1870, 3796
HindII	GTYRAC	3 cut(s)	1489, 1870, 3796
HindIII	AAGCTT	2 cut(s)	160, 2063
HpaII	CCGG	6 cut(s)	751, 1165, 2125, 2794, 4319, 4419
Hpy166II	GTNNAC	7 cut(s)	835, 1489, 1600, 1870, 2498, 3358, 3796
Hpy8I	GTNNAC	7 cut(s)	835, 1489, 1600, 1870, 2498, 3358, 3796
Hpy99I	CGWCG	3 cut(s)	70, 89, 98
HpyCH4IV	ACGT	3 cut(s)	1039, 1392, 3127
HpySE526I	ACGT	3 cut(s)	1039, 1392, 3127
Hsp92I	GRCGYC	1 cut(s)	2341
HspAI	GCGC	3 cut(s)	1808, 4206, 4451
Ksp22I	TGATCA	1 cut(s)	3984
MaeI	CTAG	7 cut(s)	951, 1368, 1463, 3527, 3791, 4037, 4773
MaeII	ACGT	3 cut(s)	1039, 1392, 3127
MbiI	CCGCTC	1 cut(s)	60
MfeI	CAATTG	4 cut(s)	466, 2105, 3332, 4062
MflI	RGATCY	6 cut(s)	454, 2469, 3004, 3322, 4042, 4626
MhlI	GDGCHC	6 cut(s)	192, 1947, 2500, 3519, 3766, 4715
MlsI	TGGCCA	1 cut(s)	3213
MluNI	TGGCCA	1 cut(s)	3213
MmeI	TCCRAC	7 cut(s)	286, 499, 1244, 1882, 2118, 3167, 4240
Mox20I	TGGCCA	1 cut(s)	3213
Mph1103I	ATGCAT	1 cut(s)	1918
MroXI	GAANNNNTTC	1 cut(s)	2627
MscI	TGGCCA	1 cut(s)	3213
MslI	CAYNNNNRTG	7 cut(s)	513, 1063, 1074, 2957, 3468, 3510, 4485
Msp20I	TGGCCA	1 cut(s)	3213
MspA1I	CMGCKG	5 cut(s)	37, 3169, 4183, 4762, 4820
MspCI	CTTAAG	1 cut(s)	1973
MspI	CCGG	6 cut(s)	751, 1165, 2125, 2794, 4319, 4419
MunI	CAATTG	4 cut(s)	466, 2105, 3332, 4062
Mva1269I	GAATGC	1 cut(s)	849
MvnI	CGCG	2 cut(s)	1622, 2814
NcoI	CCATGG	1 cut(s)	449
NdeI	CATATG	4 cut(s)	746, 1096, 1555, 1776
NmeAIII	GCCGAG	1 cut(s)	3841
NmuCI	GTSAC	9 cut(s)	15, 890, 986, 1056, 2910, 3136, 4160, 4312, 4831
NruI	TCGCGA	1 cut(s)	2814
NsbI	TGCGCA	2 cut(s)	1809, 4452
NsiI	ATGCAT	1 cut(s)	1918
NspI	RCATGY	5 cut(s)	1341, 1582, 2331, 3509, 3835
OliI	CACNNNNGTG	2 cut(s)	513, 4485
PaeI	GCATGC	1 cut(s)	3509
PaeR7I	CTCGAG	1 cut(s)	1666
PagI	TCATGA	1 cut(s)	2938
PasI	CCCWGGG	1 cut(s)	3519
PciI	ACATGT	1 cut(s)	2327
PcsI	WCGNNNNNNNCGW	2 cut(s)	90, 2338
PctI	GAATGC	1 cut(s)	849
PdmI	GAANNNNTTC	1 cut(s)	2627
PfeI	GAWTC	6 cut(s)	309, 1189, 1436, 2892, 3991, 4589
PflFI	GACNNNGTC	1 cut(s)	2608
PfoI	TCCNGGA	1 cut(s)	1468
PinAI	ACCGGT	1 cut(s)	2124
Ple19I	CGATCG	1 cut(s)	2839
PmaCI	CACGTG	1 cut(s)	1040
PmlI	CACGTG	1 cut(s)	1040
Ppu21I	YACGTR	1 cut(s)	1040
PpuMI	RGGWCCY	1 cut(s)	1466
PscI	ACATGT	1 cut(s)	2327
PsiI	TTATAA	1 cut(s)	900
Psp1406I	AACGTT	1 cut(s)	1392
Psp5II	RGGWCCY	1 cut(s)	1466
PspCI	CACGTG	1 cut(s)	1040
PspEI	GGTNACC	1 cut(s)	3960
PspFI	CCCAGC	4 cut(s)	144, 674, 4175, 4208
PspOMI	GGGCCC	1 cut(s)	3515
PspPPI	RGGWCCY	1 cut(s)	1466
PspXI	VCTCGAGB	1 cut(s)	1666
PsrI	GAACNNNNNNTAC	2 cut(s)	2868, 2900
PstNI	CAGNNNCTG	3 cut(s)	19, 1121, 4823
PsuI	RGATCY	6 cut(s)	454, 2469, 3004, 3322, 4042, 4626
PsyI	GACNNNGTC	1 cut(s)	2608
PvuI	CGATCG	1 cut(s)	2839
PvuII	CAGCTG	5 cut(s)	37, 3169, 4183, 4762, 4820
RruI	TCGCGA	1 cut(s)	2814
RsaI	GTAC	6 cut(s)	743, 3656, 3979, 4157, 4518, 4742
RsaNI	GTAC	6 cut(s)	742, 3655, 3978, 4156, 4517, 4741
RseI	CAYNNNNRTG	7 cut(s)	513, 1063, 1074, 2957, 3468, 3510, 4485
ScaI	AGTACT	1 cut(s)	4742
SduI	GDGCHC	6 cut(s)	192, 1947, 2500, 3519, 3766, 4715
SfcI	CTRYAG	4 cut(s)	642, 2946, 3315, 3684
Sfr274I	CTCGAG	1 cut(s)	1666
SinI	GGWCC	7 cut(s)	200, 265, 1295, 1466, 1584, 1904, 4013
SlaI	CTCGAG	1 cut(s)	1666
SmiMI	CAYNNNNRTG	7 cut(s)	513, 1063, 1074, 2957, 3468, 3510, 4485
SmlI	CTYRAG	3 cut(s)	1666, 1961, 1973
SmoI	CTYRAG	3 cut(s)	1666, 1961, 1973
SphI	GCATGC	1 cut(s)	3509
SspI	AATATT	3 cut(s)	583, 1891, 4249
SspMI	CTAG	7 cut(s)	951, 1368, 1463, 3527, 3791, 4037, 4773
StyI	CCWWGG	4 cut(s)	196, 449, 2043, 4602
TaiI	ACGT	3 cut(s)	1042, 1395, 3130
TatI	WGTACW	3 cut(s)	741, 4516, 4740
TauI	GCSGC	2 cut(s)	60, 1142
TfiI	GAWTC	6 cut(s)	309, 1189, 1436, 2892, 3991, 4589
TseFI	GTSAC	9 cut(s)	15, 890, 986, 1056, 2910, 3136, 4160, 4312, 4831
Tsp45I	GTSAC	9 cut(s)	15, 890, 986, 1056, 2910, 3136, 4160, 4312, 4831
TspGWI	ACGGA	3 cut(s)	150, 169, 3892
Tth111I	GACNNNGTC	1 cut(s)	2608
Vha464I	CTTAAG	1 cut(s)	1973
VneI	GTGCAC	1 cut(s)	2496
VpaK11BI	GGWCC	7 cut(s)	200, 265, 1295, 1466, 1584, 1904, 4013
XagI	CCTNNNNNAGG	2 cut(s)	3204, 3350
XapI	RAATTY	4 cut(s)	128, 1145, 2451, 2659
XceI	RCATGY	5 cut(s)	1341, 1582, 2331, 3509, 3835
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1560
XhoI	CTCGAG	1 cut(s)	1666
XmnI	GAANNNNTTC	1 cut(s)	2627
XspI	CTAG	7 cut(s)	951, 1368, 1463, 3527, 3791, 4037, 4773
ZrmI	AGTACT	1 cut(s)	4742
Zsp2I	ATGCAT	1 cut(s)	1918

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.