AT2G45960

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001159
Physical Location & Seq
Forward (+)
18910081 .. 18911982
1902 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45960.2

Sequence Viewer

Length: 861 bp
ATGGAAGGTAAAGAAGAAGATGTTAGAGTCGGAGCTAACAAGTTTCCGGAGAGGCAACCGATCGGAACTTCGGCTCAGAGTGACAAGGACTACAAAGAGCCACCACCTGCGCCGTTGTTCGAGCCCGGCGAGCTAGCTTCATGGTCCTTCTGGAGAGCTGGGATTGCTGAGTTTATAGCTACGTTTTTGTTCCTGTACATCACTGTTTTGACTGTTATGGGTGTGAAGAGGTCACCGAACATGTGTGCTTCCGTCGGAATCCAAGGTATCGCTTGGGCTTTCGGTGGTATGATCTTCGCTCTCGTCTACTGCACCGCTGGTATCTCCGGTGGACACATCAACCCAGCGGTTACGTTCGGTTTGTTCTTAGCTAGGAAGCTTTCGCTCACACGAGCTGTGTACTACATAGTGATGCAGTGCTTAGGAGCTATCTGTGGAGCTGGTGTGGTCAAGGGGTTCCAGCCAAAGCAATACCAGGCTTTGGGAGGTGGAGCCAACACCATAGCTCATGGCTACACCAAAGGAAGTGGTCTTGGAGCTGAGATTATTGGAACCTTTGTCCTTGTTTACACCGTCTTCTCTGCCACTGATGCCAAGAGAAACGCTCGTGACTCTCATGTTCCTATTCTAGCACCGCTCCCTATCGGATTCGCTGTGTTCTTGGTTCACTTAGCAACCATCCCCATTACTGGAACTGGAATCAACCCAGCAAGAAGTCTTGGAGCTGCAATCATCTTCAACAAGGACAACGCTTGGGATGACCACTGGGTCTTTTGGGTTGGACCATTCATTGGTGCTGCACTTGCTGCTCTCTACCACGTTATAGTCATCAGAGCCATCCCATTCAAGTCCAGAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

286

Amino Acids

30.6

Weight (kDa)

9.16

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 44 - 255 1.2e-73 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 115
AasI GACNNNNNNGTC 1 cut(s) 767
Acc36I ACCTGC 1 cut(s) 115
AccB7I CCANNNNNTGG 2 cut(s) 481, 791
AccBSI CCGCTC 1 cut(s) 637
AccI GTMKAC 1 cut(s) 306
AccIII TCCGGA 1 cut(s) 46
AciI CCGC 3 cut(s) 315, 347, 635
AfaI GTAC 2 cut(s) 197, 401
AfiI CCNNNNNNNGG 3 cut(s) 481, 689, 791
AflIII ACRYGT 1 cut(s) 240
AgsI TTSAA 2 cut(s) 739, 847
AjnI CCWGG 1 cut(s) 474
Aor13HI TCCGGA 1 cut(s) 46
ApeKI GCWGC 3 cut(s) 725, 797, 806
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 2 cut(s) 144, 782
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 1 cut(s) 225
AsuNHI GCTAGC 1 cut(s) 133
AvaII GGWCC 2 cut(s) 144, 782
BanII GRGCYC 1 cut(s) 126
BauI CACGAG 2 cut(s) 390, 606
BbsI GAAGAC 1 cut(s) 568
BbvI GCAGC 3 cut(s) 712, 784, 793
BccI CCATC 2 cut(s) 686, 845
BceAI ACGGC 1 cut(s) 97
BciT130I CCWGG 1 cut(s) 476
BcnI CCSGG 1 cut(s) 126
BfaI CTAG 3 cut(s) 134, 372, 629
BfuAI ACCTGC 1 cut(s) 115
BisI GCNGC 3 cut(s) 726, 798, 807
BlsI GCNGC 3 cut(s) 727, 799, 808
Bme1390I CCNGG 2 cut(s) 126, 476
Bme18I GGWCC 2 cut(s) 144, 782
BmgT120I GGNCC 2 cut(s) 144, 782
BmiI GGNNCC 3 cut(s) 458, 493, 553
BmrFI CCNGG 2 cut(s) 126, 476
BmrI ACTGGG 1 cut(s) 775
BmsI GCATC 2 cut(s) 402, 580
BmtI GCTAGC 1 cut(s) 137
BmuI ACTGGG 1 cut(s) 775
BpiI GAAGAC 1 cut(s) 568
BplI GAGNNNNNCTC 2 cut(s) 589, 621
BpmI CTGGAG 1 cut(s) 172
Bpu10I CCTNAGC 1 cut(s) 421
BpuMI CCSGG 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 262
BsaWI WCCGGW 2 cut(s) 46, 326
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 3 cut(s) 481, 689, 791
Bse1I ACTGG 3 cut(s) 694, 700, 770
BseAI TCCGGA 1 cut(s) 46
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 3 cut(s) 678, 763, 837
BseLI CCNNNNNNNGG 3 cut(s) 481, 689, 791
BseMII CTCAG 3 cut(s) 89, 159, 531
BseNI ACTGG 3 cut(s) 694, 700, 770
BseXI GCAGC 3 cut(s) 712, 784, 793
BseYI CCCAGC 3 cut(s) 158, 343, 706
BsgI GTGCAG 2 cut(s) 295, 783
Bsh1285I CGRYCG 1 cut(s) 63
BsiEI CGRYCG 1 cut(s) 63
BsiSI CCGG 3 cut(s) 47, 126, 327
BslI CCNNNNNNNGG 3 cut(s) 481, 689, 791
Bsp1286I GDGCHC 1 cut(s) 126
Bsp13I TCCGGA 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 195
Bsp143I GATC 2 cut(s) 60, 291
BspACI CCGC 3 cut(s) 315, 347, 635
BspCNI CTCAG 3 cut(s) 88, 160, 532
BspEI TCCGGA 1 cut(s) 46
BspLI GGNNCC 3 cut(s) 458, 493, 553
BspMI ACCTGC 1 cut(s) 115
BspOI GCTAGC 1 cut(s) 137
BsrBI CCGCTC 1 cut(s) 637
BsrGI TGTACA 1 cut(s) 195
BsrI ACTGG 3 cut(s) 694, 700, 770
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 2 cut(s) 60, 291
BssSI CACGAG 2 cut(s) 390, 606
BssT1I CCWWGG 1 cut(s) 262
Bst2BI CACGAG 2 cut(s) 390, 606
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 3 cut(s) 205, 214, 574
Bst6I CTCTTC 1 cut(s) 221
BstAPI GCANNNNNTGC 1 cut(s) 806
BstAUI TGTACA 1 cut(s) 195
BstC8I GCNNGC 2 cut(s) 131, 135
BstDEI CTNAG 6 cut(s) 75, 168, 367, 421, 540, 670
BstEII GGTNACC 1 cut(s) 231
BstF5I GGATG 3 cut(s) 678, 763, 837
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 2 cut(s) 63, 294
BstMBI GATC 2 cut(s) 60, 291
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 5 cut(s) 130, 164, 590, 803, 806
BstNI CCWGG 1 cut(s) 476
BstNSI RCATGY 1 cut(s) 244
BstPI GGTNACC 1 cut(s) 231
BstSCI CCNGG 2 cut(s) 124, 474
BstV1I GCAGC 3 cut(s) 712, 784, 793
BstV2I GAAGAC 1 cut(s) 568
BtsCI GGATG 3 cut(s) 678, 763, 837
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 4 cut(s) 201, 422, 585, 763
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 2 cut(s) 131, 135
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 144, 782
Csp6I GTAC 2 cut(s) 196, 400
CspCI CAANNNNNGTGG 2 cut(s) 508, 543
CviAII CATG 4 cut(s) 141, 241, 509, 617
CviQI GTAC 2 cut(s) 196, 400
DdeI CTNAG 6 cut(s) 75, 168, 367, 421, 540, 670
DpnI GATC 2 cut(s) 62, 293
DpnII GATC 2 cut(s) 60, 291
DrdI GACNNNNNNGTC 1 cut(s) 767
DseDI GACNNNNNNGTC 1 cut(s) 767
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 2 cut(s) 144, 782
Eco91I GGTNACC 1 cut(s) 231
EcoO65I GGTNACC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 262
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 262
FaeI CATG 4 cut(s) 144, 244, 512, 620
FatI CATG 4 cut(s) 140, 240, 508, 616
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 3 cut(s) 726, 798, 807
FokI GGATG 3 cut(s) 665, 770, 824
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 3 cut(s) 726, 798, 807
FspBI CTAG 3 cut(s) 134, 372, 629
GlaI GCGC 1 cut(s) 111
GluI GCNGC 3 cut(s) 726, 798, 807
GsaI CCCAGC 3 cut(s) 162, 347, 710
GsuI CTGGAG 1 cut(s) 172
HapII CCGG 3 cut(s) 47, 126, 327
HhaI GCGC 1 cut(s) 112
Hin1II CATG 4 cut(s) 144, 244, 512, 620
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 5 cut(s) 27, 258, 611, 648, 699
HpaII CCGG 3 cut(s) 47, 126, 327
HphI GGTGA 1 cut(s) 225
Hpy166II GTNNAC 5 cut(s) 307, 332, 400, 568, 667
Hpy188I TCNGA 6 cut(s) 32, 65, 78, 257, 647, 833
Hpy188III TCNNGA 4 cut(s) 47, 151, 608, 852
Hpy8I GTNNAC 5 cut(s) 307, 332, 400, 568, 667
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 1 cut(s) 157
HpyCH4III ACNGT 3 cut(s) 205, 214, 574
HpyCH4IV ACGT 3 cut(s) 182, 353, 819
HpyCH4V TGCA 4 cut(s) 312, 415, 728, 800
HpyF10VI GCNNNNNNNGC 5 cut(s) 130, 164, 590, 803, 806
HpyF3I CTNAG 6 cut(s) 75, 168, 367, 421, 540, 670
HpySE526I ACGT 3 cut(s) 182, 353, 819
Hsp92II CATG 4 cut(s) 144, 244, 512, 620
HspAI GCGC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 46
Kzo9I GATC 2 cut(s) 60, 291
LmnI GCTCC 7 cut(s) 32, 425, 437, 491, 536, 642, 722
Lsp1109I GCAGC 3 cut(s) 712, 784, 793
LweI GCATC 2 cut(s) 402, 580
MaeI CTAG 3 cut(s) 134, 372, 629
MaeII ACGT 3 cut(s) 182, 353, 819
MaeIII GTNAC 4 cut(s) 80, 231, 349, 608
MalI GATC 2 cut(s) 62, 293
MbiI CCGCTC 1 cut(s) 637
MboI GATC 2 cut(s) 60, 291
MboII GAAGA 6 cut(s) 26, 29, 238, 286, 568, 727
MhlI GDGCHC 1 cut(s) 126
MlyI GAGTC 2 cut(s) 36, 605
MmeI TCCRAC 3 cut(s) 10, 235, 760
MnlI CCTC 3 cut(s) 45, 222, 479
MroI TCCGGA 1 cut(s) 46
MslI CAYNNNNRTG 1 cut(s) 410
MspA1I CMGCKG 2 cut(s) 317, 347
MspI CCGG 3 cut(s) 47, 126, 327
MspR9I CCNGG 2 cut(s) 126, 476
MvaI CCWGG 1 cut(s) 476
MwoI GCNNNNNNNGC 5 cut(s) 130, 164, 590, 803, 806
NciI CCSGG 1 cut(s) 126
NdeII GATC 2 cut(s) 60, 291
NheI GCTAGC 1 cut(s) 133
NlaIII CATG 4 cut(s) 144, 244, 512, 620
NlaIV GGNNCC 3 cut(s) 458, 493, 553
NmuCI GTSAC 3 cut(s) 80, 231, 608
NspI RCATGY 1 cut(s) 244
PaqCI CACCTGC 1 cut(s) 115
PciI ACATGT 1 cut(s) 240
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 3 cut(s) 258, 648, 699
PflMI CCANNNNNTGG 2 cut(s) 481, 791
PkrI GCNGC 3 cut(s) 727, 799, 808
Ple19I CGATCG 1 cut(s) 63
PleI GAGTC 2 cut(s) 35, 605
PpsI GAGTC 2 cut(s) 35, 605
PscI ACATGT 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 474
PspEI GGTNACC 1 cut(s) 231
PspFI CCCAGC 3 cut(s) 158, 343, 706
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 3 cut(s) 458, 493, 553
PspPI GGNCC 2 cut(s) 144, 782
PvuI CGATCG 1 cut(s) 63
RsaI GTAC 2 cut(s) 197, 401
RsaNI GTAC 2 cut(s) 196, 400
RseI CAYNNNNRTG 1 cut(s) 410
SatI GCNGC 3 cut(s) 726, 798, 807
Sau3AI GATC 2 cut(s) 60, 291
Sau96I GGNCC 2 cut(s) 144, 782
SchI GAGTC 2 cut(s) 36, 605
ScrFI CCNGG 2 cut(s) 126, 476
SduI GDGCHC 1 cut(s) 126
SfaNI GCATC 2 cut(s) 402, 580
SinI GGWCC 2 cut(s) 144, 782
SmiMI CAYNNNNRTG 1 cut(s) 410
SsiI CCGC 3 cut(s) 315, 347, 635
SspMI CTAG 3 cut(s) 134, 372, 629
StyD4I CCNGG 2 cut(s) 124, 474
StyI CCWWGG 1 cut(s) 262
TaaI ACNGT 3 cut(s) 205, 214, 574
TaiI ACGT 3 cut(s) 185, 356, 822
TaqI TCGA 1 cut(s) 120
TatI WGTACW 2 cut(s) 195, 399
TfiI GAWTC 3 cut(s) 258, 648, 699
TscAI CASTG 4 cut(s) 208, 422, 592, 770
TseFI GTSAC 3 cut(s) 80, 231, 608
TseI GCWGC 3 cut(s) 725, 797, 806
Tsp45I GTSAC 3 cut(s) 80, 231, 608
TspDTI ATGAA 2 cut(s) 129, 778
TspGWI ACGGA 1 cut(s) 241
TspRI CASTG 4 cut(s) 208, 422, 592, 770
Van91I CCANNNNNTGG 2 cut(s) 481, 791
VpaK11BI GGWCC 2 cut(s) 144, 782
XceI RCATGY 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 3 cut(s) 134, 372, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.