pycom01g09330

Major intrinsic protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001159
Physical Location & Seq
Forward (+)
10284985 .. 10287070
2086 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g09330.2

Sequence Viewer

Length: 840 bp
ATGGAGGGTAAAGAAGAGGATGTGAAGCTGGGAGCCAACAAATTCTCAGAGAGGCAGCCCATAGGGACCTCAGCACAGACAGACAAGGACTACAAGGAGCCACCGCCGGCGCCGCTGTTCGAGCCCGGCGAGCTCAAGTCCTGGTCTTTCTGGAGGGCTGGGATTGCTGAGTTCATGGCCACCTTCCTCTTCCTCTACATCACTGTCCTCACTGTCATGGGGGTTTCCAGGGCACCCAACAAGTGTGCCTCTGTGGGCATTCAGGGCATTGCATGGGCCTTTGGTGGGACCATCTTTGCCCTTGTTTATTGTACTGCTGGTGTCTCAGGTGGTCACATAAACCCAGCTGTGACATTTGGTCTCTTCCTAGCAAGGAAGCTCTCCCTGACCAGATCCGTGTTCTACATCATCATGCAAAGCCTTGGCGCAATGGCTGGTGCCGGGGTGGTGAAGGGGTTCCAAAAGAAGCAGTATGAATTGCTCGGCGGTGGAGCTAATGTGGTGAACCATGGCTATACCAAGGGTGACGGCCTTGGCGCTGAGATTGTTGGCACTTTTGTTCTTGTGTACACTGTTTTCTCTGCCACTGATGCCAAGAGAAATGCCAGAGACTCTCATGTCCCTGTAAGGAAATGCAGATCTTGGCTCCACTCCCCATTGGGTTTGCTGTATTTTTGGTGCATTTGGCAACCATCCCCATCACCGGAACTGGCATCAACCCTGCTAGGAGTCTCGGGGCTGCCATCATCTACAACAAGGGCCATGCATGGGATGATCATTGGATCTTCTGGGTCGGACCATTCATCGGAGCTGCACTTGCTGCACTCTACCATCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

280

Amino Acids

30.02

Weight (kDa)

9.14

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 617
AccB1I GGYRCC 3 cut(s) 109, 232, 437
AciI CCGC 3 cut(s) 104, 113, 486
AclWI GGATC 2 cut(s) 387, 790
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 1 cut(s) 41
AcyI GRCGYC 1 cut(s) 110
AfaI GTAC 2 cut(s) 313, 569
AfiI CCNNNNNNNGG 4 cut(s) 285, 703, 768, 805
AhdI GACNNNNNGTC 1 cut(s) 357
AjnI CCWGG 2 cut(s) 140, 227
AluBI AGCT 6 cut(s) 28, 133, 347, 379, 494, 811
AluI AGCT 6 cut(s) 28, 133, 347, 379, 494, 811
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 4 cut(s) 328, 365, 603, 736
AlwI GGATC 2 cut(s) 387, 790
Ama87I CYCGRG 1 cut(s) 733
AoxI GGCC 4 cut(s) 177, 276, 529, 759
ApeKI GCWGC 4 cut(s) 55, 739, 811, 820
ApoI RAATTY 1 cut(s) 41
Asp700I GAANNNNTTC 1 cut(s) 455
AspLEI GCGC 3 cut(s) 112, 428, 539
AspS9I GGNCC 5 cut(s) 66, 276, 288, 759, 796
AsuC2I CCSGG 2 cut(s) 126, 442
AsuHPI GGTGA 4 cut(s) 460, 514, 536, 693
AvaI CYCGRG 1 cut(s) 733
AvaII GGWCC 3 cut(s) 66, 288, 796
BaeGI GKGCMC 1 cut(s) 235
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 3 cut(s) 109, 232, 437
BanII GRGCYC 2 cut(s) 126, 135
Bbv12I GWGCWC 1 cut(s) 135
BbvCI CCTCAGC 1 cut(s) 70
BbvI GCAGC 4 cut(s) 67, 726, 798, 807
BccI CCATC 5 cut(s) 299, 700, 706, 751, 839
BceAI ACGGC 1 cut(s) 544
BciT130I CCWGG 2 cut(s) 142, 229
BclI TGATCA 1 cut(s) 774
BcnI CCSGG 2 cut(s) 126, 442
BcoDI GTCTC 4 cut(s) 328, 365, 603, 736
BfaI CTAG 2 cut(s) 368, 725
BfoI RGCGCY 2 cut(s) 113, 540
BglII AGATCT 1 cut(s) 638
BisI GCNGC 5 cut(s) 56, 113, 740, 812, 821
BlsI GCNGC 5 cut(s) 57, 114, 741, 813, 822
Bme1390I CCNGG 4 cut(s) 126, 142, 229, 442
Bme18I GGWCC 3 cut(s) 66, 288, 796
BmeRI GACNNNNNGTC 1 cut(s) 357
BmeT110I CYCGRG 1 cut(s) 733
BmgT120I GGNCC 5 cut(s) 66, 276, 288, 759, 796
BmiI GGNNCC 9 cut(s) 34, 67, 99, 111, 234, 289, 439, 458, 647
BmrFI CCNGG 4 cut(s) 126, 142, 229, 442
BmsI GCATC 2 cut(s) 580, 722
BpmI CTGGAG 1 cut(s) 172
Bpu10I CCTNAGC 1 cut(s) 70
BpuEI CTTGAG 1 cut(s) 119
BpuMI CCSGG 2 cut(s) 126, 442
BsaHI GRCGYC 1 cut(s) 110
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 6 cut(s) 228, 421, 441, 508, 519, 532
BsaWI WCCGGW 1 cut(s) 703
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 4 cut(s) 285, 703, 768, 805
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 1 cut(s) 714
Bse3DI GCAATG 2 cut(s) 267, 435
BseBI CCWGG 2 cut(s) 142, 229
BseDI CCNNGG 6 cut(s) 228, 421, 441, 508, 519, 532
BseGI GGATG 3 cut(s) 25, 692, 777
BseLI CCNNNNNNNGG 4 cut(s) 285, 703, 768, 805
BseMI GCAATG 2 cut(s) 267, 435
BseMII CTCAG 5 cut(s) 60, 84, 159, 339, 531
BseNI ACTGG 1 cut(s) 714
BseSI GKGCMC 1 cut(s) 235
BseXI GCAGC 4 cut(s) 67, 726, 798, 807
BseYI CCCAGC 3 cut(s) 28, 158, 343
BsgI GTGCAG 2 cut(s) 797, 806
BshFI GGCC 4 cut(s) 179, 278, 531, 761
BshNI GGYRCC 3 cut(s) 109, 232, 437
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 733
BsiSI CCGG 4 cut(s) 107, 126, 441, 704
BslFI GGGAC 3 cut(s) 79, 301, 605
BslI CCNNNNNNNGG 4 cut(s) 285, 703, 768, 805
BsmAI GTCTC 4 cut(s) 328, 365, 603, 736
BsmFI GGGAC 3 cut(s) 79, 301, 605
BsmI GAATGC 1 cut(s) 258
BsnI GGCC 4 cut(s) 179, 278, 531, 761
Bso31I GGTCTC 1 cut(s) 365
BsoBI CYCGRG 1 cut(s) 733
Bsp1286I GDGCHC 3 cut(s) 126, 135, 235
Bsp1407I TGTACA 1 cut(s) 567
Bsp143I GATC 4 cut(s) 392, 638, 774, 782
Bsp19I CCATGG 1 cut(s) 508
BspACI CCGC 3 cut(s) 104, 113, 486
BspANI GGCC 4 cut(s) 179, 278, 531, 761
BspCNI CTCAG 5 cut(s) 59, 83, 160, 338, 532
BspLI GGNNCC 9 cut(s) 34, 67, 99, 111, 234, 289, 439, 458, 647
BspPI GGATC 2 cut(s) 387, 790
BspT107I GGYRCC 3 cut(s) 109, 232, 437
BspTNI GGTCTC 1 cut(s) 365
BsrDI GCAATG 2 cut(s) 267, 435
BsrFI RCCGGY 1 cut(s) 106
BsrGI TGTACA 1 cut(s) 567
BsrI ACTGG 1 cut(s) 714
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 6 cut(s) 228, 421, 441, 508, 519, 532
BssMI GATC 4 cut(s) 392, 638, 774, 782
BssNI GRCGYC 1 cut(s) 110
BssT1I CCWWGG 4 cut(s) 421, 508, 519, 532
Bst2UI CCWGG 2 cut(s) 142, 229
Bst4CI ACNGT 3 cut(s) 205, 214, 574
Bst6I CTCTTC 3 cut(s) 9, 194, 368
BstACI GRCGYC 1 cut(s) 110
BstAPI GCANNNNNTGC 1 cut(s) 820
BstAUI TGTACA 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 108, 131
BstDEI CTNAG 5 cut(s) 46, 70, 168, 325, 540
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 3 cut(s) 25, 692, 777
BstH2I RGCGCY 2 cut(s) 113, 540
BstHHI GCGC 3 cut(s) 112, 428, 539
BstKTI GATC 4 cut(s) 395, 641, 777, 785
BstMAI GTCTC 4 cut(s) 328, 365, 603, 736
BstMBI GATC 4 cut(s) 392, 638, 774, 782
BstMWI GCNNNNNNNGC 8 cut(s) 112, 121, 130, 164, 264, 590, 817, 820
BstNI CCWGG 2 cut(s) 142, 229
BstSCI CCNGG 4 cut(s) 124, 140, 227, 440
BstSLI GKGCMC 1 cut(s) 235
BstV1I GCAGC 4 cut(s) 67, 726, 798, 807
BstX2I RGATCY 3 cut(s) 392, 638, 782
BstYI RGATCY 3 cut(s) 392, 638, 782
BsuRI GGCC 4 cut(s) 179, 278, 531, 761
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 3 cut(s) 25, 692, 777
BtsIMutI CAGTG 4 cut(s) 201, 210, 570, 585
Cac8I GCNNGC 2 cut(s) 108, 131
CfoI GCGC 3 cut(s) 112, 428, 539
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 5 cut(s) 66, 276, 288, 759, 796
Csp6I GTAC 2 cut(s) 312, 568
CviAII CATG 8 cut(s) 175, 217, 273, 412, 509, 617, 763, 767
CviQI GTAC 2 cut(s) 312, 568
DdeI CTNAG 5 cut(s) 46, 70, 168, 325, 540
DinI GGCGCC 1 cut(s) 111
DpnI GATC 4 cut(s) 394, 640, 776, 784
DpnII GATC 4 cut(s) 392, 638, 774, 782
DrdI GACNNNNNNGTC 1 cut(s) 617
DriI GACNNNNNGTC 1 cut(s) 357
DseDI GACNNNNNNGTC 1 cut(s) 617
EaeI YGGCCR 1 cut(s) 177
Eam1104I CTCTTC 3 cut(s) 9, 194, 368
Eam1105I GACNNNNNGTC 1 cut(s) 357
EarI CTCTTC 3 cut(s) 9, 194, 368
Ecl136II GAGCTC 1 cut(s) 133
Eco130I CCWWGG 4 cut(s) 421, 508, 519, 532
Eco24I GRGCYC 2 cut(s) 126, 135
Eco31I GGTCTC 1 cut(s) 365
Eco47I GGWCC 3 cut(s) 66, 288, 796
Eco53kI GAGCTC 1 cut(s) 133
Eco88I CYCGRG 1 cut(s) 733
EcoICRI GAGCTC 1 cut(s) 133
EcoO109I RGGNCCY 1 cut(s) 66
EcoRII CCWGG 2 cut(s) 140, 227
EcoT14I CCWWGG 4 cut(s) 421, 508, 519, 532
EcoT22I ATGCAT 1 cut(s) 768
EcoT38I GRGCYC 2 cut(s) 126, 135
EgeI GGCGCC 1 cut(s) 111
EheI GGCGCC 1 cut(s) 111
ErhI CCWWGG 4 cut(s) 421, 508, 519, 532
FaeI CATG 8 cut(s) 178, 220, 276, 415, 512, 620, 766, 770
FaqI GGGAC 3 cut(s) 79, 301, 605
FatI CATG 8 cut(s) 174, 216, 272, 411, 508, 616, 762, 766
FbaI TGATCA 1 cut(s) 774
Fnu4HI GCNGC 5 cut(s) 56, 113, 740, 812, 821
FokI GGATG 3 cut(s) 32, 679, 784
FriOI GRGCYC 2 cut(s) 126, 135
Fsp4HI GCNGC 5 cut(s) 56, 113, 740, 812, 821
FspBI CTAG 2 cut(s) 368, 725
GlaI GCGC 3 cut(s) 111, 427, 538
GluI GCNGC 5 cut(s) 56, 113, 740, 812, 821
GsaI CCCAGC 3 cut(s) 32, 162, 347
GsuI CTGGAG 1 cut(s) 172
HaeII RGCGCY 2 cut(s) 113, 540
HaeIII GGCC 4 cut(s) 179, 278, 531, 761
HapII CCGG 4 cut(s) 107, 126, 441, 704
HhaI GCGC 3 cut(s) 112, 428, 539
Hin1I GRCGYC 1 cut(s) 110
Hin1II CATG 8 cut(s) 178, 220, 276, 415, 512, 620, 766, 770
Hin6I GCGC 3 cut(s) 110, 426, 537
HinP1I GCGC 3 cut(s) 110, 426, 537
HinfI GANTC 2 cut(s) 611, 729
HpaII CCGG 4 cut(s) 107, 126, 441, 704
HphI GGTGA 4 cut(s) 460, 514, 536, 693
Hpy166II GTNNAC 3 cut(s) 505, 568, 570
Hpy188I TCNGA 4 cut(s) 49, 796, 808, 836
Hpy188III TCNNGA 1 cut(s) 151
Hpy8I GTNNAC 3 cut(s) 505, 568, 570
HpyAV CCTTC 2 cut(s) 193, 445
HpyCH4III ACNGT 3 cut(s) 205, 214, 574
HpyCH4V TGCA 7 cut(s) 272, 415, 636, 681, 766, 814, 823
HpyF10VI GCNNNNNNNGC 8 cut(s) 112, 121, 130, 164, 264, 590, 817, 820
HpyF3I CTNAG 5 cut(s) 46, 70, 168, 325, 540
Hsp92I GRCGYC 1 cut(s) 110
Hsp92II CATG 8 cut(s) 178, 220, 276, 415, 512, 620, 766, 770
HspAI GCGC 3 cut(s) 110, 426, 537
KasI GGCGCC 1 cut(s) 109
KroI GCCGGC 1 cut(s) 106
KroNI GCCGGC 1 cut(s) 108
Ksp22I TGATCA 1 cut(s) 774
Kzo9I GATC 4 cut(s) 392, 638, 774, 782
LmnI GCTCC 5 cut(s) 32, 97, 491, 651, 808
Lsp1109I GCAGC 4 cut(s) 67, 726, 798, 807
LweI GCATC 2 cut(s) 580, 722
MaeI CTAG 2 cut(s) 368, 725
MaeIII GTNAC 3 cut(s) 332, 349, 524
MalI GATC 4 cut(s) 394, 640, 776, 784
MboI GATC 4 cut(s) 392, 638, 774, 782
MboII GAAGA 4 cut(s) 26, 181, 355, 777
MflI RGATCY 3 cut(s) 392, 638, 782
MhlI GDGCHC 3 cut(s) 126, 135, 235
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 2 cut(s) 41, 476
MluNI TGGCCA 1 cut(s) 179
Mly113I GGCGCC 1 cut(s) 110
MlyI GAGTC 2 cut(s) 605, 738
MmeI TCCRAC 1 cut(s) 774
MnlI CCTC 8 cut(s) 10, 45, 79, 147, 197, 203, 218, 259
Mox20I TGGCCA 1 cut(s) 179
Mph1103I ATGCAT 1 cut(s) 768
MreI CGCCGGCG 1 cut(s) 106
MroNI GCCGGC 1 cut(s) 106
MroXI GAANNNNTTC 1 cut(s) 455
MscI TGGCCA 1 cut(s) 179
MslI CAYNNNNRTG 2 cut(s) 215, 410
Msp20I TGGCCA 1 cut(s) 179
MspA1I CMGCKG 2 cut(s) 115, 347
MspI CCGG 4 cut(s) 107, 126, 441, 704
MspR9I CCNGG 4 cut(s) 126, 142, 229, 442
Mva1269I GAATGC 1 cut(s) 258
MvaI CCWGG 2 cut(s) 142, 229
MwoI GCNNNNNNNGC 8 cut(s) 112, 121, 130, 164, 264, 590, 817, 820
NaeI GCCGGC 1 cut(s) 108
NarI GGCGCC 1 cut(s) 110
NciI CCSGG 2 cut(s) 126, 442
NcoI CCATGG 1 cut(s) 508
NdeII GATC 4 cut(s) 392, 638, 774, 782
NgoMIV GCCGGC 1 cut(s) 106
NlaIII CATG 8 cut(s) 178, 220, 276, 415, 512, 620, 766, 770
NlaIV GGNNCC 9 cut(s) 34, 67, 99, 111, 234, 289, 439, 458, 647
NmeAIII GCCGAG 1 cut(s) 462
NmuCI GTSAC 3 cut(s) 332, 349, 524
NsiI ATGCAT 1 cut(s) 768
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 258
PdiI GCCGGC 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 455
PkrI GCNGC 5 cut(s) 57, 114, 741, 813, 822
PleI GAGTC 2 cut(s) 605, 737
PluTI GGCGCC 1 cut(s) 113
PpsI GAGTC 2 cut(s) 605, 737
PpuMI RGGWCCY 1 cut(s) 66
Psp124BI GAGCTC 1 cut(s) 135
Psp5II RGGWCCY 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 140, 227
PspFI CCCAGC 3 cut(s) 28, 158, 343
PspGI CCWGG 2 cut(s) 140, 227
PspN4I GGNNCC 9 cut(s) 34, 67, 99, 111, 234, 289, 439, 458, 647
PspPI GGNCC 5 cut(s) 66, 276, 288, 759, 796
PspPPI RGGWCCY 1 cut(s) 66
PsuI RGATCY 3 cut(s) 392, 638, 782
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 2 cut(s) 313, 569
RsaNI GTAC 2 cut(s) 312, 568
RseI CAYNNNNRTG 2 cut(s) 215, 410
SacI GAGCTC 1 cut(s) 135
SatI GCNGC 5 cut(s) 56, 113, 740, 812, 821
Sau3AI GATC 4 cut(s) 392, 638, 774, 782
Sau96I GGNCC 5 cut(s) 66, 276, 288, 759, 796
SchI GAGTC 2 cut(s) 605, 738
ScrFI CCNGG 4 cut(s) 126, 142, 229, 442
SduI GDGCHC 3 cut(s) 126, 135, 235
SetI ASST 9 cut(s) 30, 71, 135, 185, 331, 349, 381, 496, 813
SfaNI GCATC 2 cut(s) 580, 722
SfoI GGCGCC 1 cut(s) 111
SgrAI CRCCGGYG 1 cut(s) 106
SinI GGWCC 3 cut(s) 66, 288, 796
SmiMI CAYNNNNRTG 2 cut(s) 215, 410
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 2 cut(s) 41, 476
SsiI CCGC 3 cut(s) 104, 113, 486
SspDI GGCGCC 1 cut(s) 109
SspMI CTAG 2 cut(s) 368, 725
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 4 cut(s) 124, 140, 227, 440
StyI CCWWGG 4 cut(s) 421, 508, 519, 532
TaaI ACNGT 3 cut(s) 205, 214, 574
TaqI TCGA 1 cut(s) 120
TasI AATT 2 cut(s) 41, 476
TatI WGTACW 2 cut(s) 311, 567
TauI GCSGC 1 cut(s) 115
TscAI CASTG 4 cut(s) 208, 217, 577, 592
TseFI GTSAC 3 cut(s) 332, 349, 524
TseI GCWGC 4 cut(s) 55, 739, 811, 820
Tsp45I GTSAC 3 cut(s) 332, 349, 524
TspDTI ATGAA 3 cut(s) 163, 489, 792
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 4 cut(s) 208, 217, 577, 592
VpaK11BI GGWCC 3 cut(s) 66, 288, 796
XapI RAATTY 1 cut(s) 41
XmnI GAANNNNTTC 1 cut(s) 455
XspI CTAG 2 cut(s) 368, 725
Zsp2I ATGCAT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.