AT2G47050

Invertase pectin methylesterase inhibitor family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003768
Physical Location & Seq
Reverse (-)
19331545 .. 19332659
1115 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G47050.1

Sequence Viewer

Length: 651 bp
ATGAACACTCCAATAAAACTCGCCTTTCTCATTCTCTGCATTGCCCTAACCGCAACCGCATTTATAGTCCCAGCAAAACGTGACGCCGTTTCACCACACCACCAAAAAGCCGTCGATGGAATCTGCAGCGTTGTGCAAGACAAACGTCTATGCAGCATTACCTTAAGAAACGTCCCAAGCGATGATCCCGCCGTTTTGGTTCGTTACTTAGCCACGGCAGCAGAAGCGTCCGTTAAAAAGGGTTTGAAGTTCCTCTCCGGAATCAAACCAAAATACAAAGGAAACGCCTTCGCCACAACATGCATCACCGGCTGCGAGAAACAGCTACACAACGCCTTGGACGACTTTGCAGATTTCTGGAAAGCCGCAGGAAAAAATCTAACGAGCATGGCTGAGAATTACTTCACATGTAAGAAGAAGATGACTTCCATCTTCACTTACCATTCCACTTGTCTCGATGACATTTACGACAAGACGTTGCACAAAGTGGTCGAAGGAGGGATTGGCCTTGGGAAAAGAATGAGTGGTGAGTCTGTGGATGTGTTCGCTGGAATGGGCAAAGTCTTTAACACTTTCAACATTAAGACCAAACTTAACCAGAAAGATACCGACACGTTGCTCCCACCACCTTTGTCCTTTTACTACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

216

Amino Acids

23.78

Weight (kDa)

9.16

Isoelectric Point (pI)

29.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 162 2.9e-11 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 257
AciI CCGC 4 cut(s) 51, 57, 189, 366
AclWI GGATC 1 cut(s) 179
AcyI GRCGYC 1 cut(s) 84
AdeI CACNNNGTG 1 cut(s) 487
AflII CTTAAG 1 cut(s) 163
AflIII ACRYGT 2 cut(s) 407, 612
AgsI TTSAA 2 cut(s) 247, 577
AjuI GAANNNNNNNTTGG 2 cut(s) 486, 518
AluBI AGCT 1 cut(s) 325
AluI AGCT 1 cut(s) 325
Alw26I GTCTC 1 cut(s) 458
AlwI GGATC 1 cut(s) 179
Aor13HI TCCGGA 1 cut(s) 257
AoxI GGCC 1 cut(s) 505
ApeKI GCWGC 4 cut(s) 126, 153, 218, 312
Asp700I GAANNNNTTC 1 cut(s) 401
AsuHPI GGTGA 3 cut(s) 84, 298, 539
BbvI GCAGC 4 cut(s) 138, 165, 230, 299
BccI CCATC 2 cut(s) 110, 437
BceAI ACGGC 4 cut(s) 71, 95, 176, 231
BcoDI GTCTC 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 124
BfrI CTTAAG 1 cut(s) 163
BisI GCNGC 5 cut(s) 127, 154, 219, 313, 366
BlsI GCNGC 5 cut(s) 128, 155, 220, 314, 367
BmsI GCATC 1 cut(s) 312
BoxI GACNNNNGTC 1 cut(s) 144
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 3 cut(s) 213, 336, 508
BsaWI WCCGGW 1 cut(s) 257
Bse118I RCCGGY 1 cut(s) 308
Bse3DI GCAATG 1 cut(s) 39
BseAI TCCGGA 1 cut(s) 257
BseDI CCNNGG 3 cut(s) 213, 336, 508
BseGI GGATG 1 cut(s) 544
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 1 cut(s) 384
BseXI GCAGC 4 cut(s) 138, 165, 230, 299
BseYI CCCAGC 1 cut(s) 70
BshFI GGCC 1 cut(s) 507
BsiSI CCGG 2 cut(s) 258, 309
BslFI GGGAC 2 cut(s) 53, 158
BsmAI GTCTC 1 cut(s) 458
BsmFI GGGAC 2 cut(s) 53, 158
BsnI GGCC 1 cut(s) 507
Bsp13I TCCGGA 1 cut(s) 257
Bsp143I GATC 1 cut(s) 184
BspACI CCGC 4 cut(s) 51, 57, 189, 366
BspANI GGCC 1 cut(s) 507
BspCNI CTCAG 1 cut(s) 385
BspEI TCCGGA 1 cut(s) 257
BspMAI CTGCAG 1 cut(s) 128
BspPI GGATC 1 cut(s) 179
BspTI CTTAAG 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 39
BsrFI RCCGGY 1 cut(s) 308
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 3 cut(s) 213, 336, 508
BssMI GATC 1 cut(s) 184
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 2 cut(s) 336, 508
BstACI GRCGYC 1 cut(s) 84
BstAFI CTTAAG 1 cut(s) 163
BstDEI CTNAG 2 cut(s) 208, 393
BstDSI CCRYGG 1 cut(s) 213
BstF5I GGATG 1 cut(s) 544
BstKTI GATC 1 cut(s) 187
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 1 cut(s) 184
BstMWI GCNNNNNNNGC 4 cut(s) 50, 218, 224, 309
BstNSI RCATGY 2 cut(s) 303, 411
BstPAI GACNNNNGTC 1 cut(s) 144
BstSFI CTRYAG 1 cut(s) 124
BstV1I GCAGC 4 cut(s) 138, 165, 230, 299
BsuRI GGCC 1 cut(s) 507
BtgI CCRYGG 1 cut(s) 213
BtgZI GCGATG 1 cut(s) 195
BtsCI GGATG 1 cut(s) 544
Cfr10I RCCGGY 1 cut(s) 308
CseI GACGC 2 cut(s) 92, 216
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviAII CATG 3 cut(s) 300, 388, 408
CviJI RGCY 7 cut(s) 110, 212, 312, 325, 365, 392, 507
CviKI_1 RGCY 7 cut(s) 110, 212, 312, 325, 365, 392, 507
DdeI CTNAG 2 cut(s) 208, 393
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
DraIII CACNNNGTG 1 cut(s) 487
Eco130I CCWWGG 2 cut(s) 336, 508
EcoT14I CCWWGG 2 cut(s) 336, 508
EcoT22I ATGCAT 1 cut(s) 305
ErhI CCWWGG 2 cut(s) 336, 508
FaeI CATG 3 cut(s) 303, 391, 411
FaiI YATR 5 cut(s) 65, 151, 301, 389, 409
FaqI GGGAC 2 cut(s) 53, 158
FatI CATG 3 cut(s) 299, 387, 407
FauI CCCGC 1 cut(s) 196
Fnu4HI GCNGC 5 cut(s) 127, 154, 219, 313, 366
FokI GGATG 1 cut(s) 551
Fsp4HI GCNGC 5 cut(s) 127, 154, 219, 313, 366
GluI GCNGC 5 cut(s) 127, 154, 219, 313, 366
GsaI CCCAGC 1 cut(s) 74
HaeIII GGCC 1 cut(s) 507
HapII CCGG 2 cut(s) 258, 309
HgaI GACGC 2 cut(s) 92, 216
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 3 cut(s) 303, 391, 411
HinfI GANTC 3 cut(s) 120, 261, 530
HpaII CCGG 2 cut(s) 258, 309
HphI GGTGA 3 cut(s) 84, 298, 539
Hpy188III TCNNGA 3 cut(s) 258, 358, 455
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 2 cut(s) 298, 488
HpyCH4IV ACGT 5 cut(s) 79, 145, 171, 476, 614
HpyCH4V TGCA 7 cut(s) 39, 126, 136, 153, 303, 350, 481
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 218, 224, 309
HpyF3I CTNAG 2 cut(s) 208, 393
HpySE526I ACGT 5 cut(s) 79, 145, 171, 476, 614
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 3 cut(s) 303, 391, 411
Kpn2I TCCGGA 1 cut(s) 257
Kzo9I GATC 1 cut(s) 184
LmnI GCTCC 1 cut(s) 624
LpnPI CCDG 7 cut(s) 84, 271, 322, 343, 354, 534, 611
Lsp1109I GCAGC 4 cut(s) 138, 165, 230, 299
LweI GCATC 1 cut(s) 312
MaeII ACGT 5 cut(s) 79, 145, 171, 476, 614
MaeIII GTNAC 2 cut(s) 80, 203
MalI GATC 1 cut(s) 186
MboI GATC 1 cut(s) 184
MboII GAAGA 3 cut(s) 424, 427, 430
MluCI AATT 1 cut(s) 397
MlyI GAGTC 1 cut(s) 539
MnlI CCTC 2 cut(s) 263, 491
Mph1103I ATGCAT 1 cut(s) 305
MroI TCCGGA 1 cut(s) 257
MroXI GAANNNNTTC 1 cut(s) 401
MseI TTAA 5 cut(s) 164, 234, 567, 582, 594
MspCI CTTAAG 1 cut(s) 163
MspI CCGG 2 cut(s) 258, 309
MwoI GCNNNNNNNGC 4 cut(s) 50, 218, 224, 309
NdeII GATC 1 cut(s) 184
NlaIII CATG 3 cut(s) 303, 391, 411
NmuCI GTSAC 1 cut(s) 80
NsiI ATGCAT 1 cut(s) 305
NspI RCATGY 2 cut(s) 303, 411
PciI ACATGT 1 cut(s) 407
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 339
PdmI GAANNNNTTC 1 cut(s) 401
PfeI GAWTC 2 cut(s) 120, 261
PkrI GCNGC 5 cut(s) 128, 155, 220, 314, 367
PleI GAGTC 1 cut(s) 538
PpsI GAGTC 1 cut(s) 538
PscI ACATGT 1 cut(s) 407
PshAI GACNNNNGTC 1 cut(s) 144
PspFI CCCAGC 1 cut(s) 70
PstI CTGCAG 1 cut(s) 128
SaqAI TTAA 5 cut(s) 164, 234, 567, 582, 594
SatI GCNGC 5 cut(s) 127, 154, 219, 313, 366
Sau3AI GATC 1 cut(s) 184
SchI GAGTC 1 cut(s) 539
SetI ASST 8 cut(s) 82, 148, 164, 174, 327, 479, 617, 631
SfaNI GCATC 1 cut(s) 312
SfcI CTRYAG 1 cut(s) 124
SmlI CTYRAG 1 cut(s) 163
SmoI CTYRAG 1 cut(s) 163
Sse9I AATT 1 cut(s) 397
SsiI CCGC 4 cut(s) 51, 57, 189, 366
StyI CCWWGG 2 cut(s) 336, 508
TaiI ACGT 5 cut(s) 82, 148, 174, 479, 617
TaqI TCGA 3 cut(s) 114, 456, 492
TasI AATT 1 cut(s) 397
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 2 cut(s) 120, 261
Tru1I TTAA 5 cut(s) 164, 234, 567, 582, 594
Tru9I TTAA 5 cut(s) 164, 234, 567, 582, 594
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 4 cut(s) 126, 153, 218, 312
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 220
Vha464I CTTAAG 1 cut(s) 163
XceI RCATGY 2 cut(s) 303, 411
XmnI GAANNNNTTC 1 cut(s) 401
Zsp2I ATGCAT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.