Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0003768

Physical Location & Seq

Genomic Coordinates Reverse (-)

31188980 .. 31190602

Gene Structure 1623 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG308600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGAAGCTGGTGAACCAAATGTGTGATTATACAGACCACCTGGCGGAGTGTCAGCAATCTCTTAGCCCGGTGGCTCAAAACAGCAGCGCTGGTTTCAAGGACTACATGAAGGCTGCATTGCTTTCCATCTCCGCAGAGGCTCAGAAATCCTTCGACATCACAGAGTCTCTCTTGGTTGACGCCAAAAATGGCACCAGAGTCAAGAAGTCCATTGAGGAGTGCAAGGAGCTAATGAAAGAAGCCCGTGAGGAGCTACAGGCCTTGGTCTCATTCGTCGGTGATGCTGAGTTGCACACCATGACAGACCGCGCCTTGGAGCTGAAAATCTGGATAGGTAGTGTGAACAGTTATGCCGGAACATGCAGGGATGTAATGGGAGATGACGACCCTAAGGCTTCGGAGGCAATGAAAGGACCCATAGATGACATTACCGCAATCACCGACAACGCTCTGGCCATTATGGGACAGATTTCTAATATCCTCGGATGGTTTGGCGTCAAGCTAAATGAGAAGGCAATCATTGAAAAAGCCAGCAACAGCAGGCGGCTTATGGGTGAAACTCTAGAGGCAGATATCCCCATAGATGGGTTCCCAAGCTGGATGCCCAGCGCAGACCGTAAGCTTATGGCTAAGCAAGACAATGGCAGGATTGCGCCCAATGTTGTTGTAGCTCAAGATGGCAGCGGGCAATACAAGACCATTAACGAGGCACTCATGGCGTACCCTAAGGGCAACCAAGGACGCTTCGTTATCTATGTGAAGAAGGGAGTGTACAAGGAGACTGTCATCATAAACAAAAAGATGCCCAACATATACATGTATGGTGATGGCCCCAGAGCCACTATCATCACTGGTGACAAGGGAGAGGCGAACACCGGAGATAAAATATCTACGAGCGGCACCTTCCAAGTGTACGCTAACGGATTTATTGGGAGGTCAATGGGATTCACCAACACCGCCGGTCCGGAAGCAAGACCAGCCGTGGCTCTTCGCACTTCCGGCGACATGATTGGCATCTTCAACAGTAGAATTAGTGGATACCAAGACACCTTGTACATGAATATTGGACGTCAGTTCTATAGGAACTGCGTCATTTCAGGACACGTGGATTATATCTTCGGGGATGCTCGAGTAGTTATCCAAAACTCTTTAATCATCGTGAGGAAGTCGTCCCTCCCGCACCAGAACCAAGCCTTCATCACCGCTCCAGGGCAACAAACCGCACACGAAGTCGGGGCTACCGTGCTCCACAACTGCAGGATCGTGCCGGAGAGGGCACTCTTCCCCACGAGGTTCGAAGTGAGAACATACTTGGGGAGGCCATGGAAGACCTACGCTAGAGCTGCGTTCATGGAGTGCACCATGGCTGACTTTATCAGACCTGAAGGGGTTGTCGCATGGGACGGTAAGGACTTTAACACCGAACACGCCTTCTTTGGTGAGTTCAAGAACAAGGGACCTGGTGCCAACTTAGCCAGCAGGGTGAAATGGGCAAAGGGCGGCTTCACTGGCGAGATGGATAGGAATACAGCTGCGAAATTTACCGTCGATAGCATGCTACTACCAGACACATGGTTGAAGCAGGCTACTATTCCTTACTTACCTAGCTTCGAACTTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

540

Amino Acids

59.54

Weight (kDa)

7.56

Isoelectric Point (pI)

33.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	2 - 153	1.6e-16	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	220 - 520	3.8e-101	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003768)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47030 AT2G47040 AT2G47050 AT3G62170 AT3G62180 AT3G62180
fragaria_vesca	FvH4_1g26360 FvH4_4g21500
malus_domestica	MD16G1136400.v1.1 MD17G1283800.v1.1
prunus_persica	Prupe.1G105400_v2.0.a1 Prupe.3G003700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0486131 RchiOBHm_Chr4g0427511
rosa_laevigata	RLG00000007232 RLG00000023101
rosa_multiflora	Rmu_sc0001006.1_g000002 Rmu_sc0009627.1_g000001 Rmu_sc0017895.1_g000001 Rmu_sc0017895.1_g000002
rosa_roxburghii	Rroxscaffold_5G00369150 Rroxscaffold_6G00396030
rosa_rugosa	Rorug03G0223700 Rorug04G0218200
rosa_samantha	Rh3AG273500 Rh3BG308600 Rh3CG307100 Rh3DG303400 Rh4AG274700 Rh4BG280500 Rh4CG295800 Rh4DG277900
rosa_wichuraiana	Rw3G024240 Rw4G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1072
AccB1I	GGYRCC	3 cut(s)	191, 899, 1463
AccBSI	CCGCTC	2 cut(s)	897, 1205
AccII	CGCG	1 cut(s)	309
AccIII	TCCGGA	1 cut(s)	964
AclWI	GGATC	1 cut(s)	1268
AcoI	YGGCCR	1 cut(s)	453
AcsI	RAATTY	1 cut(s)	1538
AcuI	CTGAAG	1 cut(s)	1404
AcvI	CACGTG	1 cut(s)	1105
AcyI	GRCGYC	3 cut(s)	180, 495, 1069
AfaI	GTAC	4 cut(s)	722, 773, 914, 1055
AfeI	AGCGCT	1 cut(s)	88
AfiI	CCNNNNNNNGG	5 cut(s)	43, 313, 584, 585, 1209
AflIII	ACRYGT	2 cut(s)	816, 1102
AgsI	TTSAA	5 cut(s)	97, 524, 1021, 1447, 1579
AjnI	CCWGG	3 cut(s)	39, 1207, 1459
Alw21I	GWGCWC	2 cut(s)	1248, 1361
Alw26I	GTCTC	3 cut(s)	171, 271, 773
Alw44I	GTGCAC	1 cut(s)	1357
AlwI	GGATC	1 cut(s)	1268
Ama87I	CYCGRG	1 cut(s)	1128
Aor13HI	TCCGGA	1 cut(s)	964
Aor51HI	AGCGCT	1 cut(s)	88
AoxI	GGCC	4 cut(s)	258, 453, 829, 1319
ApaLI	GTGCAC	1 cut(s)	1357
ApeKI	GCWGC	5 cut(s)	84, 113, 681, 1343, 1532
ApoI	RAATTY	1 cut(s)	1538
Asp700I	GAANNNNTTC	1 cut(s)	149
AspLEI	GCGC	4 cut(s)	89, 311, 611, 655
AspS9I	GGNCC	4 cut(s)	413, 830, 962, 1457
AsuC2I	CCSGG	1 cut(s)	68
AsuII	TTCGAA	2 cut(s)	1296, 1611
AvaI	CYCGRG	1 cut(s)	1128
AvaII	GGWCC	3 cut(s)	413, 962, 1457
AxyI	CCTNAGG	2 cut(s)	390, 726
BaeGI	GKGCMC	2 cut(s)	1279, 1361
BalI	TGGCCA	1 cut(s)	455
BanI	GGYRCC	3 cut(s)	191, 899, 1463
BarI	GAAGNNNNNNTAC	2 cut(s)	755, 787
BauI	CACGAG	1 cut(s)	1288
BbrPI	CACGTG	1 cut(s)	1105
BbsI	GAAGAC	1 cut(s)	1334
Bbv12I	GWGCWC	2 cut(s)	1248, 1361
BbvI	GCAGC	5 cut(s)	96, 100, 693, 1330, 1519
BccI	CCATC	6 cut(s)	134, 480, 578, 671, 821, 1510
BceAI	ACGGC	1 cut(s)	965
BciT130I	CCWGG	3 cut(s)	41, 1209, 1461
BciVI	GTATCC	1 cut(s)	1031
BcnI	CCSGG	1 cut(s)	68
BcoDI	GTCTC	3 cut(s)	171, 271, 773
BfaI	CTAG	3 cut(s)	563, 1338, 1605
BfmI	CTRYAG	3 cut(s)	254, 1078, 1255
BfoI	RGCGCY	1 cut(s)	90
BfuI	GTATCC	1 cut(s)	1031
BisI	GCNGC	8 cut(s)	85, 114, 545, 682, 898, 1344, 1501, 1533
BlpI	GCTNAGC	1 cut(s)	630
BlsI	GCNGC	8 cut(s)	86, 115, 546, 683, 899, 1345, 1502, 1534
Bme1390I	CCNGG	4 cut(s)	41, 68, 1209, 1461
Bme18I	GGWCC	3 cut(s)	413, 962, 1457
BmeT110I	CYCGRG	1 cut(s)	1128
BmgT120I	GGNCC	4 cut(s)	413, 830, 962, 1457
BmiI	GGNNCC	7 cut(s)	193, 415, 590, 832, 901, 1458, 1465
BmrFI	CCNGG	4 cut(s)	41, 68, 1209, 1461
BmsI	GCATC	5 cut(s)	271, 591, 792, 1023, 1114
BpiI	GAAGAC	1 cut(s)	1334
BpmI	CTGGAG	1 cut(s)	1191
Bpu1102I	GCTNAGC	1 cut(s)	630
Bpu14I	TTCGAA	2 cut(s)	1296, 1611
BpuEI	CTTGAG	1 cut(s)	657
BpuMI	CCSGG	1 cut(s)	68
BsaAI	YACGTR	1 cut(s)	1105
BsaBI	GATNNNNATC	1 cut(s)	1013
BsaHI	GRCGYC	3 cut(s)	180, 495, 1069
BsaI	GGTCTC	1 cut(s)	271
BsaJI	CCNNGG	8 cut(s)	261, 312, 481, 736, 981, 1208, 1322, 1362
BsaWI	WCCGGW	2 cut(s)	875, 964
Bsc4I	CCNNNNNNNGG	5 cut(s)	43, 313, 584, 585, 1209
Bse118I	RCCGGY	1 cut(s)	959
Bse1I	ACTGG	2 cut(s)	856, 1513
Bse21I	CCTNAGG	2 cut(s)	390, 726
Bse3DI	GCAATG	2 cut(s)	116, 411
Bse8I	GATNNNNATC	1 cut(s)	1013
BseAI	TCCGGA	1 cut(s)	964
BseBI	CCWGG	3 cut(s)	41, 1209, 1461
BseDI	CCNNGG	8 cut(s)	261, 312, 481, 736, 981, 1208, 1322, 1362
BseGI	GGATG	4 cut(s)	373, 491, 606, 1129
BseJI	GATNNNNATC	1 cut(s)	1013
BseLI	CCNNNNNNNGG	5 cut(s)	43, 313, 584, 585, 1209
BseMI	GCAATG	2 cut(s)	116, 411
BseMII	CTCAG	2 cut(s)	155, 276
BseNI	ACTGG	2 cut(s)	856, 1513
BseRI	GAGGAG	2 cut(s)	230, 263
BseSI	GKGCMC	2 cut(s)	1279, 1361
BseXI	GCAGC	5 cut(s)	96, 100, 693, 1330, 1519
BseYI	CCCAGC	1 cut(s)	605
Bsh1236I	CGCG	1 cut(s)	309
BshFI	GGCC	4 cut(s)	260, 455, 831, 1321
BshNI	GGYRCC	3 cut(s)	191, 899, 1463
BsiHKAI	GWGCWC	2 cut(s)	1248, 1361
BsiHKCI	CYCGRG	1 cut(s)	1128
BsiSI	CCGG	7 cut(s)	68, 354, 876, 960, 965, 999, 1268
BslFI	GGGAC	4 cut(s)	477, 1156, 1415, 1470
BslI	CCNNNNNNNGG	5 cut(s)	43, 313, 584, 585, 1209
BsmAI	GTCTC	3 cut(s)	171, 271, 773
BsmFI	GGGAC	4 cut(s)	477, 1156, 1415, 1470
BsnI	GGCC	4 cut(s)	260, 455, 831, 1321
Bso31I	GGTCTC	1 cut(s)	271
BsoBI	CYCGRG	1 cut(s)	1128
Bsp119I	TTCGAA	2 cut(s)	1296, 1611
Bsp1286I	GDGCHC	3 cut(s)	1248, 1279, 1361
Bsp13I	TCCGGA	1 cut(s)	964
Bsp1407I	TGTACA	2 cut(s)	771, 1053
Bsp143I	GATC	1 cut(s)	1260
Bsp1720I	GCTNAGC	1 cut(s)	630
Bsp19I	CCATGG	2 cut(s)	1322, 1362
BspANI	GGCC	4 cut(s)	260, 455, 831, 1321
BspCNI	CTCAG	2 cut(s)	154, 277
BspEI	TCCGGA	1 cut(s)	964
BspFNI	CGCG	1 cut(s)	309
BspLI	GGNNCC	7 cut(s)	193, 415, 590, 832, 901, 1458, 1465
BspMAI	CTGCAG	1 cut(s)	1259
BspPI	GGATC	1 cut(s)	1268
BspQI	GCTCTTC	1 cut(s)	993
BspT104I	TTCGAA	2 cut(s)	1296, 1611
BspT107I	GGYRCC	3 cut(s)	191, 899, 1463
BspTNI	GGTCTC	1 cut(s)	271
BsrBI	CCGCTC	2 cut(s)	897, 1205
BsrDI	GCAATG	2 cut(s)	116, 411
BsrFI	RCCGGY	1 cut(s)	959
BsrGI	TGTACA	2 cut(s)	771, 1053
BsrI	ACTGG	2 cut(s)	856, 1513
BssAI	RCCGGY	1 cut(s)	959
BssECI	CCNNGG	8 cut(s)	261, 312, 481, 736, 981, 1208, 1322, 1362
BssMI	GATC	1 cut(s)	1260
BssNI	GRCGYC	3 cut(s)	180, 495, 1069
BssSI	CACGAG	1 cut(s)	1288
BssT1I	CCWWGG	5 cut(s)	261, 312, 736, 1322, 1362
Bst2BI	CACGAG	1 cut(s)	1288
Bst2UI	CCWGG	3 cut(s)	41, 1209, 1461
Bst4CI	ACNGT	7 cut(s)	347, 617, 784, 1025, 1243, 1406, 1546
Bst6I	CTCTTC	2 cut(s)	993, 1286
BstACI	GRCGYC	3 cut(s)	180, 495, 1069
BstAUI	TGTACA	2 cut(s)	771, 1053
BstBAI	YACGTR	1 cut(s)	1105
BstBI	TTCGAA	2 cut(s)	1296, 1611
BstC8I	GCNNGC	6 cut(s)	532, 542, 686, 1477, 1556, 1584
BstDEI	CTNAG	8 cut(s)	62, 141, 285, 390, 630, 726, 1471, 1620
BstDSI	CCRYGG	3 cut(s)	981, 1322, 1362
BstF5I	GGATG	4 cut(s)	373, 491, 606, 1129
BstFNI	CGCG	1 cut(s)	309
BstH2I	RGCGCY	1 cut(s)	90
BstHHI	GCGC	4 cut(s)	89, 311, 611, 655
BstKTI	GATC	1 cut(s)	1263
BstMAI	GTCTC	3 cut(s)	171, 271, 773
BstMBI	GATC	1 cut(s)	1260
BstMWI	GCNNNNNNNGC	7 cut(s)	401, 716, 977, 999, 1343, 1472, 1509
BstNI	CCWGG	3 cut(s)	41, 1209, 1461
BstNSI	RCATGY	3 cut(s)	363, 820, 1558
BstSCI	CCNGG	4 cut(s)	39, 66, 1207, 1459
BstSFI	CTRYAG	3 cut(s)	254, 1078, 1255
BstSLI	GKGCMC	2 cut(s)	1279, 1361
BstUI	CGCG	1 cut(s)	309
BstV1I	GCAGC	5 cut(s)	96, 100, 693, 1330, 1519
BstV2I	GAAGAC	1 cut(s)	1334
BstXI	CCANNNNNNTGG	1 cut(s)	1572
Bsu36I	CCTNAGG	2 cut(s)	390, 726
BsuI	GTATCC	1 cut(s)	1031
BsuRI	GGCC	4 cut(s)	260, 455, 831, 1321
BtgI	CCRYGG	3 cut(s)	981, 1322, 1362
BtsCI	GGATG	4 cut(s)	373, 491, 606, 1129
BtsIMutI	CAGTG	2 cut(s)	849, 1506
Cac8I	GCNNGC	6 cut(s)	532, 542, 686, 1477, 1556, 1584
CfoI	GCGC	4 cut(s)	89, 311, 611, 655
Cfr10I	RCCGGY	1 cut(s)	959
Cfr13I	GGNCC	4 cut(s)	413, 830, 962, 1457
CpoI	CGGWCCG	1 cut(s)	962
CseI	GACGC	4 cut(s)	188, 484, 750, 1078
CsiI	ACCWGGT	1 cut(s)	1459
Csp6I	GTAC	4 cut(s)	721, 772, 913, 1054
CspI	CGGWCCG	1 cut(s)	962
CviQI	GTAC	4 cut(s)	721, 772, 913, 1054
DdeI	CTNAG	8 cut(s)	62, 141, 285, 390, 630, 726, 1471, 1620
DpnI	GATC	1 cut(s)	1262
DpnII	GATC	1 cut(s)	1260
EaeI	YGGCCR	1 cut(s)	453
Eam1104I	CTCTTC	2 cut(s)	993, 1286
EarI	CTCTTC	2 cut(s)	993, 1286
EciI	GGCGGA	1 cut(s)	59
Eco130I	CCWWGG	5 cut(s)	261, 312, 736, 1322, 1362
Eco147I	AGGCCT	1 cut(s)	260
Eco31I	GGTCTC	1 cut(s)	271
Eco32I	GATATC	1 cut(s)	574
Eco47I	GGWCC	3 cut(s)	413, 962, 1457
Eco47III	AGCGCT	1 cut(s)	88
Eco57I	CTGAAG	1 cut(s)	1404
Eco72I	CACGTG	1 cut(s)	1105
Eco81I	CCTNAGG	2 cut(s)	390, 726
Eco88I	CYCGRG	1 cut(s)	1128
EcoO109I	RGGNCCY	2 cut(s)	413, 1457
EcoRII	CCWGG	3 cut(s)	39, 1207, 1459
EcoRV	GATATC	1 cut(s)	574
EcoT14I	CCWWGG	5 cut(s)	261, 312, 736, 1322, 1362
ErhI	CCWWGG	5 cut(s)	261, 312, 736, 1322, 1362
FalI	AAGNNNNNCTT	2 cut(s)	1487, 1519
FaqI	GGGAC	4 cut(s)	477, 1156, 1415, 1470
FauI	CCCGC	2 cut(s)	677, 1185
Fnu4HI	GCNGC	8 cut(s)	85, 114, 545, 682, 898, 1344, 1501, 1533
FokI	GGATG	4 cut(s)	380, 498, 613, 1136
Fsp4HI	GCNGC	8 cut(s)	85, 114, 545, 682, 898, 1344, 1501, 1533
FspBI	CTAG	3 cut(s)	563, 1338, 1605
GlaI	GCGC	4 cut(s)	88, 310, 610, 654
GluI	GCNGC	8 cut(s)	85, 114, 545, 682, 898, 1344, 1501, 1533
GsaI	CCCAGC	1 cut(s)	609
GsuI	CTGGAG	1 cut(s)	1191
HaeII	RGCGCY	1 cut(s)	90
HaeIII	GGCC	4 cut(s)	260, 455, 831, 1321
HapII	CCGG	7 cut(s)	68, 354, 876, 960, 965, 999, 1268
HgaI	GACGC	4 cut(s)	188, 484, 750, 1078
HhaI	GCGC	4 cut(s)	89, 311, 611, 655
Hin1I	GRCGYC	3 cut(s)	180, 495, 1069
Hin6I	GCGC	4 cut(s)	87, 309, 609, 653
HinP1I	GCGC	4 cut(s)	87, 309, 609, 653
HincII	GTYRAC	1 cut(s)	178
HindII	GTYRAC	1 cut(s)	178
HindIII	AAGCTT	1 cut(s)	620
HinfI	GANTC	3 cut(s)	164, 198, 945
HpaII	CCGG	7 cut(s)	68, 354, 876, 960, 965, 999, 1268
Hpy166II	GTNNAC	6 cut(s)	13, 178, 343, 772, 913, 1359
Hpy188I	TCNGA	4 cut(s)	144, 400, 485, 1379
Hpy188III	TCNNGA	8 cut(s)	202, 328, 563, 674, 965, 1098, 1159, 1447
Hpy8I	GTNNAC	6 cut(s)	13, 178, 343, 772, 913, 1359
Hpy99I	CGWCG	2 cut(s)	278, 1550
HpyAV	CCTTC	8 cut(s)	103, 160, 505, 757, 913, 1204, 1379, 1441
HpyCH4III	ACNGT	7 cut(s)	347, 617, 784, 1025, 1243, 1406, 1546
HpyCH4IV	ACGT	2 cut(s)	1069, 1104
HpyCH4V	TGCA	6 cut(s)	116, 222, 292, 363, 1257, 1359
HpyF10VI	GCNNNNNNNGC	7 cut(s)	401, 716, 977, 999, 1343, 1472, 1509
HpyF3I	CTNAG	8 cut(s)	62, 141, 285, 390, 630, 726, 1471, 1620
HpySE526I	ACGT	2 cut(s)	1069, 1104
Hsp92I	GRCGYC	3 cut(s)	180, 495, 1069
HspAI	GCGC	4 cut(s)	87, 309, 609, 653
Kpn2I	TCCGGA	1 cut(s)	964
Kzo9I	GATC	1 cut(s)	1260
LguI	GCTCTTC	1 cut(s)	993
LmnI	GCTCC	5 cut(s)	226, 250, 316, 1210, 1251
Lsp1109I	GCAGC	5 cut(s)	96, 100, 693, 1330, 1519
LweI	GCATC	5 cut(s)	271, 591, 792, 1023, 1114
MabI	ACCWGGT	1 cut(s)	1459
MaeI	CTAG	3 cut(s)	563, 1338, 1605
MaeII	ACGT	2 cut(s)	1069, 1104
MaeIII	GTNAC	1 cut(s)	854
MalI	GATC	1 cut(s)	1262
MbiI	CCGCTC	2 cut(s)	897, 1205
MboI	GATC	1 cut(s)	1260
MboII	GAAGA	6 cut(s)	772, 980, 1009, 1108, 1273, 1339
MhlI	GDGCHC	3 cut(s)	1248, 1279, 1361
MlsI	TGGCCA	1 cut(s)	455
MluCI	AATT	2 cut(s)	1029, 1538
MluNI	TGGCCA	1 cut(s)	455
MlyI	GAGTC	2 cut(s)	173, 207
Mox20I	TGGCCA	1 cut(s)	455
MroI	TCCGGA	1 cut(s)	964
MroXI	GAANNNNTTC	1 cut(s)	149
MscI	TGGCCA	1 cut(s)	455
MseI	TTAA	3 cut(s)	702, 1151, 1416
MslI	CAYNNNNRTG	1 cut(s)	815
Msp20I	TGGCCA	1 cut(s)	455
MspA1I	CMGCKG	2 cut(s)	684, 1532
MspI	CCGG	7 cut(s)	68, 354, 876, 960, 965, 999, 1268
MspR9I	CCNGG	4 cut(s)	41, 68, 1209, 1461
MvaI	CCWGG	3 cut(s)	41, 1209, 1461
MvnI	CGCG	1 cut(s)	309
MwoI	GCNNNNNNNGC	7 cut(s)	401, 716, 977, 999, 1343, 1472, 1509
NciI	CCSGG	1 cut(s)	68
NcoI	CCATGG	2 cut(s)	1322, 1362
NdeII	GATC	1 cut(s)	1260
NlaIV	GGNNCC	7 cut(s)	193, 415, 590, 832, 901, 1458, 1465
NmuCI	GTSAC	1 cut(s)	854
NspI	RCATGY	3 cut(s)	363, 820, 1558
NspV	TTCGAA	2 cut(s)	1296, 1611
PaeI	GCATGC	1 cut(s)	1558
PaeR7I	CTCGAG	1 cut(s)	1128
PceI	AGGCCT	1 cut(s)	260
PciI	ACATGT	1 cut(s)	816
PciSI	GCTCTTC	1 cut(s)	993
PcsI	WCGNNNNNNNCGW	1 cut(s)	1239
PdmI	GAANNNNTTC	1 cut(s)	149
PfeI	GAWTC	1 cut(s)	945
PkrI	GCNGC	8 cut(s)	86, 115, 546, 683, 899, 1345, 1502, 1534
PleI	GAGTC	2 cut(s)	172, 206
PmaCI	CACGTG	1 cut(s)	1105
PmlI	CACGTG	1 cut(s)	1105
PpsI	GAGTC	2 cut(s)	172, 206
Ppu21I	YACGTR	1 cut(s)	1105
PpuMI	RGGWCCY	2 cut(s)	413, 1457
PscI	ACATGT	1 cut(s)	816
Psp5II	RGGWCCY	2 cut(s)	413, 1457
Psp6I	CCWGG	3 cut(s)	39, 1207, 1459
PspCI	CACGTG	1 cut(s)	1105
PspFI	CCCAGC	1 cut(s)	605
PspGI	CCWGG	3 cut(s)	39, 1207, 1459
PspN4I	GGNNCC	7 cut(s)	193, 415, 590, 832, 901, 1458, 1465
PspPI	GGNCC	4 cut(s)	413, 830, 962, 1457
PspPPI	RGGWCCY	2 cut(s)	413, 1457
PspXI	VCTCGAGB	1 cut(s)	1128
PstI	CTGCAG	1 cut(s)	1259
PvuII	CAGCTG	1 cut(s)	1532
RsaI	GTAC	4 cut(s)	722, 773, 914, 1055
RsaNI	GTAC	4 cut(s)	721, 772, 913, 1054
RseI	CAYNNNNRTG	1 cut(s)	815
Rsr2I	CGGWCCG	1 cut(s)	962
RsrII	CGGWCCG	1 cut(s)	962
SapI	GCTCTTC	1 cut(s)	993
SaqAI	TTAA	3 cut(s)	702, 1151, 1416
SatI	GCNGC	8 cut(s)	85, 114, 545, 682, 898, 1344, 1501, 1533
Sau3AI	GATC	1 cut(s)	1260
Sau96I	GGNCC	4 cut(s)	413, 830, 962, 1457
SchI	GAGTC	2 cut(s)	173, 207
ScrFI	CCNGG	4 cut(s)	41, 68, 1209, 1461
SduI	GDGCHC	3 cut(s)	1248, 1279, 1361
SexAI	ACCWGGT	1 cut(s)	1459
SfaNI	GCATC	5 cut(s)	271, 591, 792, 1023, 1114
SfcI	CTRYAG	3 cut(s)	254, 1078, 1255
Sfr274I	CTCGAG	1 cut(s)	1128
SfuI	TTCGAA	2 cut(s)	1296, 1611
SinI	GGWCC	3 cut(s)	413, 962, 1457
SlaI	CTCGAG	1 cut(s)	1128
SmiMI	CAYNNNNRTG	1 cut(s)	815
SmlI	CTYRAG	2 cut(s)	672, 1128
SmoI	CTYRAG	2 cut(s)	672, 1128
SphI	GCATGC	1 cut(s)	1558
Sse9I	AATT	2 cut(s)	1029, 1538
SseBI	AGGCCT	1 cut(s)	260
SspI	AATATT	1 cut(s)	1063
SspMI	CTAG	3 cut(s)	563, 1338, 1605
StuI	AGGCCT	1 cut(s)	260
StyD4I	CCNGG	4 cut(s)	39, 66, 1207, 1459
StyI	CCWWGG	5 cut(s)	261, 312, 736, 1322, 1362
TaaI	ACNGT	7 cut(s)	347, 617, 784, 1025, 1243, 1406, 1546
TaiI	ACGT	2 cut(s)	1072, 1107
TaqI	TCGA	5 cut(s)	153, 1129, 1296, 1548, 1611
TasI	AATT	2 cut(s)	1029, 1538
TatI	WGTACW	2 cut(s)	771, 1053
TauI	GCSGC	3 cut(s)	547, 900, 1503
TfiI	GAWTC	1 cut(s)	945
Tru1I	TTAA	3 cut(s)	702, 1151, 1416
Tru9I	TTAA	3 cut(s)	702, 1151, 1416
TscAI	CASTG	2 cut(s)	856, 1513
TseFI	GTSAC	1 cut(s)	854
TseI	GCWGC	5 cut(s)	84, 113, 681, 1343, 1532
Tsp45I	GTSAC	1 cut(s)	854
TspDTI	ATGAA	7 cut(s)	17, 122, 248, 422, 1073, 1186, 1339
TspGWI	ACGGA	1 cut(s)	936
TspRI	CASTG	2 cut(s)	856, 1513
VneI	GTGCAC	1 cut(s)	1357
VpaK11BI	GGWCC	3 cut(s)	413, 962, 1457
XapI	RAATTY	1 cut(s)	1538
XbaI	TCTAGA	1 cut(s)	562
XceI	RCATGY	3 cut(s)	363, 820, 1558
XhoI	CTCGAG	1 cut(s)	1128
XmnI	GAANNNNTTC	1 cut(s)	149
XspI	CTAG	3 cut(s)	563, 1338, 1605
ZraI	GACGTC	1 cut(s)	1070

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.