Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0003768

Physical Location & Seq

Genomic Coordinates Forward (+)

18109391 .. 18111234

Gene Structure 1844 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023101

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1752 bp

ATGGTAGCCAAGAAAGTACTTATATGGGGGATATCCTTTACACTTGTGGTGGGGGTGGCTATCGGAGTGGTGGCCATAGTTATTCACAACAATGCGAAGAAGCCAGGACATGGGAGCCTATCAGCGCAGATGAAGCTGGTGAACCAAATGTGTGATTATACAGACCACCGGGCGGAGTGTCATCAATCTCTTAGCCCGGTGGCTCAAAACAGCAGCGCTGGTTTCAAGGACTACATGAAGGCTGCATTGCTTTCCATCTCTGCAGAGGCGCAGAAATCCTTCAACATGACAGAGTCTCTCTTGGTTGACGCCAAAAATGGCACCAGAGTCAAGAAGTCCATTGAGGAATGCAAGGACCTAATGAAAGCAGCTCGTGAGGAGCTACAGGCCTTGGTCTCATTCATCGGTGATGCTGAGTTGTACACCATGACAGACCGCGCCTTGGAGCTGAAAATCTGGATCGGTAGTGTGAACAGTTACGCCGGAACATGCAGGGATGTATTGGGAGATGACGACCCTAAGGCTTCGGAGGCAATGAAAGGACCCATAGATGACATTACCGCAATCACCGACAACGCTCTGGCCATTATGGGACAGATTTCTAACATCCTTGGATGGTTTGGCGTCAAGCTAAATGAGAAGGCAATCATTGAAAAAGCCAGCAACAGCAGGCGGCTTATGGGTGAAACTGTAGAGGCAGATATCCCCAAAGATGGGTACCCAAGCTGGATGCCCAGCGCAGACCGTAAGCTTATGGCTAAGCAAGACAATGGCAGGATTGCGCCCAATGTTGTCGTAGCTCAAGATGGCAGCGGGCAATACAAGACCATTAACGAGGCACTCGTGGCGTACCCTAAGGGCAACCAAGGACGCTTCGTTATCTATGTGAAGAAGGGAGTGTACAAGGAGACTGTCATCATAAACAAAAAGATGCCCAACATATACATGTATGGTGATGGCCCCAGAGCCACTATCATCACTGGTGACAAGGGAGAGGCGAAAACCGGAGATAAAATATCTACAAGCGGCACCTTCCAAGTGTACGCTAACGGATTTATTGGGAGGTCAATGGGATTCACCAACACCGCCGGTCCGGAAGCAAGACCCGCCGTGGCTCTTCGCACTTCCGGCGACATGATTGGCATCTTCAACAGTAGAATTAGTGGATACCAAGACACCTTGTACATGAATATTGGACGTCAGTTCTATAGGAACTGCGTCATTTCAGGACACGTGGATTATATCTTTGGGGATGCTCGAGTGGTCATCCAAAACTCTTTAATCATCGTGAGGAAGTCGTCCCTCCCGCACCAGAACCAAGCCTTCATCACCGCTCCAGGGCAACAAACCGCACACGAAGTCGGGGCTACCGTGCTTCACAACTGCAGGATCGTGCCGGAGGAGGCACTCTTCCCCACGAGGTTCGAAGTGAGAACATACTTGGGGAGGCCATGGAAGACCTACGCTAGGGCTGCGTTCATGGAGTGCACCATGGCTGACTTTATCAGACCTGAAGGGGTTGTCGCATGGGACGGTAAGGACTTCAACACCGAACACGCCTTCTTTGGTGAGTTCAAGAACAAGGGACCTGGTGCCAACTTAGCCAACAGGGTGAAATGGGCAAAGGGCGGCTTCACCGGCGAGATGGATAGGAATACCGCTGCGAAATTTACCGTCGATAACATGCTACTACCAGACACATGGTTGAAGCAGGCTACTATTCCTTACTTACCTGGCTTCGAACGTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

584

Amino Acids

64.0

Weight (kDa)

8.94

Isoelectric Point (pI)

31.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	45 - 196	4.5e-17	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	263 - 563	1.9e-101	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003768)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47030 AT2G47040 AT2G47050 AT3G62170 AT3G62180 AT3G62180
fragaria_vesca	FvH4_1g26360 FvH4_4g21500
malus_domestica	MD16G1136400.v1.1 MD17G1283800.v1.1
prunus_persica	Prupe.1G105400_v2.0.a1 Prupe.3G003700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0486131 RchiOBHm_Chr4g0427511
rosa_laevigata	RLG00000007232 RLG00000023101
rosa_multiflora	Rmu_sc0001006.1_g000002 Rmu_sc0009627.1_g000001 Rmu_sc0017895.1_g000001 Rmu_sc0017895.1_g000002
rosa_roxburghii	Rroxscaffold_5G00369150 Rroxscaffold_6G00396030
rosa_rugosa	Rorug03G0223700 Rorug04G0218200
rosa_samantha	Rh3AG273500 Rh3BG308600 Rh3CG307100 Rh3DG303400 Rh4AG274700 Rh4BG280500 Rh4CG295800 Rh4DG277900
rosa_wichuraiana	Rw3G024240 Rw4G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1201
Acc65I	GGTACC	1 cut(s)	717
AccB1I	GGYRCC	4 cut(s)	320, 717, 1028, 1592
AccB7I	CCANNNNNTGG	1 cut(s)	110
AccBSI	CCGCTC	1 cut(s)	1334
AccII	CGCG	1 cut(s)	438
AccIII	TCCGGA	1 cut(s)	1093
AclWI	GGATC	2 cut(s)	467, 1397
AcoI	YGGCCR	2 cut(s)	72, 582
AcsI	RAATTY	1 cut(s)	1667
AcuI	CTGAAG	1 cut(s)	1533
AcvI	CACGTG	1 cut(s)	1234
AcyI	GRCGYC	3 cut(s)	309, 624, 1198
AfaI	GTAC	7 cut(s)	18, 422, 719, 851, 902, 1043, 1184
AfeI	AGCGCT	1 cut(s)	217
AfiI	CCNNNNNNNGG	7 cut(s)	110, 172, 442, 713, 714, 1338, 1467
AflIII	ACRYGT	2 cut(s)	945, 1231
AgsI	TTSAA	7 cut(s)	226, 283, 653, 1150, 1546, 1576, 1708
AjnI	CCWGG	4 cut(s)	103, 1336, 1588, 1732
AluBI	AGCT	8 cut(s)	136, 371, 382, 448, 631, 726, 751, 800
AluI	AGCT	8 cut(s)	136, 371, 382, 448, 631, 726, 751, 800
Alw21I	GWGCWC	1 cut(s)	1490
Alw26I	GTCTC	3 cut(s)	300, 400, 902
Alw44I	GTGCAC	1 cut(s)	1486
AlwI	GGATC	2 cut(s)	467, 1397
Ama87I	CYCGRG	1 cut(s)	1257
Aor13HI	TCCGGA	1 cut(s)	1093
Aor51HI	AGCGCT	1 cut(s)	217
AoxI	GGCC	5 cut(s)	72, 387, 582, 958, 1448
ApaLI	GTGCAC	1 cut(s)	1486
ApeKI	GCWGC	6 cut(s)	213, 242, 368, 810, 1472, 1661
ApoI	RAATTY	1 cut(s)	1667
Asp700I	GAANNNNTTC	1 cut(s)	278
Asp718I	GGTACC	1 cut(s)	717
AspLEI	GCGC	6 cut(s)	127, 218, 271, 440, 740, 784
AspS9I	GGNCC	5 cut(s)	355, 542, 959, 1091, 1586
AsuC2I	CCSGG	2 cut(s)	170, 197
AsuII	TTCGAA	2 cut(s)	1425, 1740
AvaI	CYCGRG	1 cut(s)	1257
AvaII	GGWCC	4 cut(s)	355, 542, 1091, 1586
AxyI	CCTNAGG	2 cut(s)	519, 855
BaeGI	GKGCMC	1 cut(s)	1490
BalI	TGGCCA	2 cut(s)	74, 584
BanI	GGYRCC	4 cut(s)	320, 717, 1028, 1592
BarI	GAAGNNNNNNTAC	2 cut(s)	884, 916
BauI	CACGAG	3 cut(s)	372, 842, 1417
BbrPI	CACGTG	1 cut(s)	1234
BbsI	GAAGAC	1 cut(s)	1463
Bbv12I	GWGCWC	1 cut(s)	1490
BbvI	GCAGC	6 cut(s)	225, 229, 380, 822, 1459, 1648
BccI	CCATC	6 cut(s)	263, 609, 707, 800, 950, 1639
BceAI	ACGGC	1 cut(s)	1094
BciT130I	CCWGG	4 cut(s)	105, 1338, 1590, 1734
BciVI	GTATCC	1 cut(s)	1160
BcnI	CCSGG	2 cut(s)	170, 197
BcoDI	GTCTC	3 cut(s)	300, 400, 902
BfaI	CTAG	1 cut(s)	1467
BfmI	CTRYAG	5 cut(s)	261, 383, 690, 1207, 1384
BfoI	RGCGCY	1 cut(s)	219
BfuI	GTATCC	1 cut(s)	1160
BisI	GCNGC	9 cut(s)	214, 243, 369, 674, 811, 1027, 1473, 1630, 1662
BlpI	GCTNAGC	1 cut(s)	759
BlsI	GCNGC	9 cut(s)	215, 244, 370, 675, 812, 1028, 1474, 1631, 1663
BmcAI	AGTACT	1 cut(s)	18
Bme1390I	CCNGG	6 cut(s)	105, 170, 197, 1338, 1590, 1734
Bme18I	GGWCC	4 cut(s)	355, 542, 1091, 1586
BmeT110I	CYCGRG	1 cut(s)	1257
BmgT120I	GGNCC	5 cut(s)	355, 542, 959, 1091, 1586
BmiI	GGNNCC	8 cut(s)	116, 322, 544, 719, 961, 1030, 1587, 1594
BmrFI	CCNGG	6 cut(s)	105, 170, 197, 1338, 1590, 1734
BmsI	GCATC	5 cut(s)	400, 720, 921, 1152, 1243
BpiI	GAAGAC	1 cut(s)	1463
BpmI	CTGGAG	1 cut(s)	1320
Bpu1102I	GCTNAGC	1 cut(s)	759
Bpu14I	TTCGAA	2 cut(s)	1425, 1740
BpuEI	CTTGAG	1 cut(s)	786
BpuMI	CCSGG	2 cut(s)	170, 197
BsaAI	YACGTR	1 cut(s)	1234
BsaBI	GATNNNNATC	1 cut(s)	1142
BsaHI	GRCGYC	3 cut(s)	309, 624, 1198
BsaI	GGTCTC	1 cut(s)	400
BsaJI	CCNNGG	8 cut(s)	390, 441, 610, 865, 1110, 1337, 1451, 1491
BsaWI	WCCGGW	2 cut(s)	1004, 1093
Bsc4I	CCNNNNNNNGG	7 cut(s)	110, 172, 442, 713, 714, 1338, 1467
Bse118I	RCCGGY	2 cut(s)	1088, 1637
Bse1I	ACTGG	1 cut(s)	985
Bse21I	CCTNAGG	2 cut(s)	519, 855
Bse3DI	GCAATG	2 cut(s)	245, 540
Bse8I	GATNNNNATC	1 cut(s)	1142
BseAI	TCCGGA	1 cut(s)	1093
BseBI	CCWGG	4 cut(s)	105, 1338, 1590, 1734
BseDI	CCNNGG	8 cut(s)	390, 441, 610, 865, 1110, 1337, 1451, 1491
BseGI	GGATG	6 cut(s)	502, 606, 620, 735, 1258, 1266
BseJI	GATNNNNATC	1 cut(s)	1142
BseLI	CCNNNNNNNGG	7 cut(s)	110, 172, 442, 713, 714, 1338, 1467
BseMI	GCAATG	2 cut(s)	245, 540
BseMII	CTCAG	1 cut(s)	405
BseNI	ACTGG	1 cut(s)	985
BseRI	GAGGAG	2 cut(s)	392, 1415
BseSI	GKGCMC	1 cut(s)	1490
BseXI	GCAGC	6 cut(s)	225, 229, 380, 822, 1459, 1648
BseYI	CCCAGC	1 cut(s)	734
Bsh1236I	CGCG	1 cut(s)	438
BshFI	GGCC	5 cut(s)	74, 389, 584, 960, 1450
BshNI	GGYRCC	4 cut(s)	320, 717, 1028, 1592
BsiHKAI	GWGCWC	1 cut(s)	1490
BsiHKCI	CYCGRG	1 cut(s)	1257
BsiSI	CCGG	9 cut(s)	169, 197, 483, 1005, 1089, 1094, 1128, 1397, 1638
BslFI	GGGAC	4 cut(s)	606, 1285, 1544, 1599
BslI	CCNNNNNNNGG	7 cut(s)	110, 172, 442, 713, 714, 1338, 1467
BsmAI	GTCTC	3 cut(s)	300, 400, 902
BsmFI	GGGAC	4 cut(s)	606, 1285, 1544, 1599
BsmI	GAATGC	1 cut(s)	353
BsnI	GGCC	5 cut(s)	74, 389, 584, 960, 1450
Bso31I	GGTCTC	1 cut(s)	400
BsoBI	CYCGRG	1 cut(s)	1257
Bsp119I	TTCGAA	2 cut(s)	1425, 1740
Bsp1286I	GDGCHC	1 cut(s)	1490
Bsp13I	TCCGGA	1 cut(s)	1093
Bsp1407I	TGTACA	3 cut(s)	420, 900, 1182
Bsp143I	GATC	2 cut(s)	459, 1389
Bsp1720I	GCTNAGC	1 cut(s)	759
Bsp19I	CCATGG	2 cut(s)	1451, 1491
BspANI	GGCC	5 cut(s)	74, 389, 584, 960, 1450
BspCNI	CTCAG	1 cut(s)	406
BspEI	TCCGGA	1 cut(s)	1093
BspFNI	CGCG	1 cut(s)	438
BspLI	GGNNCC	8 cut(s)	116, 322, 544, 719, 961, 1030, 1587, 1594
BspMAI	CTGCAG	2 cut(s)	265, 1388
BspPI	GGATC	2 cut(s)	467, 1397
BspQI	GCTCTTC	1 cut(s)	1122
BspT104I	TTCGAA	2 cut(s)	1425, 1740
BspT107I	GGYRCC	4 cut(s)	320, 717, 1028, 1592
BspTNI	GGTCTC	1 cut(s)	400
BsrBI	CCGCTC	1 cut(s)	1334
BsrDI	GCAATG	2 cut(s)	245, 540
BsrFI	RCCGGY	2 cut(s)	1088, 1637
BsrGI	TGTACA	3 cut(s)	420, 900, 1182
BsrI	ACTGG	1 cut(s)	985
BssAI	RCCGGY	2 cut(s)	1088, 1637
BssECI	CCNNGG	8 cut(s)	390, 441, 610, 865, 1110, 1337, 1451, 1491
BssMI	GATC	2 cut(s)	459, 1389
BssNI	GRCGYC	3 cut(s)	309, 624, 1198
BssSI	CACGAG	3 cut(s)	372, 842, 1417
BssT1I	CCWWGG	6 cut(s)	390, 441, 610, 865, 1451, 1491
Bst2BI	CACGAG	3 cut(s)	372, 842, 1417
Bst2UI	CCWGG	4 cut(s)	105, 1338, 1590, 1734
Bst4CI	ACNGT	8 cut(s)	476, 691, 746, 913, 1154, 1372, 1535, 1675
Bst6I	CTCTTC	2 cut(s)	1122, 1415
BstACI	GRCGYC	3 cut(s)	309, 624, 1198
BstAUI	TGTACA	3 cut(s)	420, 900, 1182
BstBAI	YACGTR	1 cut(s)	1234
BstBI	TTCGAA	2 cut(s)	1425, 1740
BstC8I	GCNNGC	4 cut(s)	661, 671, 815, 1713
BstDEI	CTNAG	7 cut(s)	191, 414, 519, 759, 855, 1600, 1749
BstDSI	CCRYGG	3 cut(s)	1110, 1451, 1491
BstENI	CCTNNNNNAGG	1 cut(s)	1465
BstF5I	GGATG	6 cut(s)	502, 606, 620, 735, 1258, 1266
BstFNI	CGCG	1 cut(s)	438
BstH2I	RGCGCY	1 cut(s)	219
BstHHI	GCGC	6 cut(s)	127, 218, 271, 440, 740, 784
BstKTI	GATC	2 cut(s)	462, 1392
BstMAI	GTCTC	3 cut(s)	300, 400, 902
BstMBI	GATC	2 cut(s)	459, 1389
BstMWI	GCNNNNNNNGC	8 cut(s)	133, 530, 845, 1106, 1128, 1472, 1601, 1638
BstNI	CCWGG	4 cut(s)	105, 1338, 1590, 1734
BstNSI	RCATGY	3 cut(s)	492, 949, 1687
BstSCI	CCNGG	6 cut(s)	103, 168, 195, 1336, 1588, 1732
BstSFI	CTRYAG	5 cut(s)	261, 383, 690, 1207, 1384
BstSLI	GKGCMC	1 cut(s)	1490
BstUI	CGCG	1 cut(s)	438
BstV1I	GCAGC	6 cut(s)	225, 229, 380, 822, 1459, 1648
BstV2I	GAAGAC	1 cut(s)	1463
BstXI	CCANNNNNNTGG	1 cut(s)	1701
Bsu36I	CCTNAGG	2 cut(s)	519, 855
BsuI	GTATCC	1 cut(s)	1160
BsuRI	GGCC	5 cut(s)	74, 389, 584, 960, 1450
BtgI	CCRYGG	3 cut(s)	1110, 1451, 1491
BtsCI	GGATG	6 cut(s)	502, 606, 620, 735, 1258, 1266
BtsIMutI	CAGTG	1 cut(s)	978
Cac8I	GCNNGC	4 cut(s)	661, 671, 815, 1713
CfoI	GCGC	6 cut(s)	127, 218, 271, 440, 740, 784
Cfr10I	RCCGGY	2 cut(s)	1088, 1637
Cfr13I	GGNCC	5 cut(s)	355, 542, 959, 1091, 1586
CpoI	CGGWCCG	1 cut(s)	1091
CseI	GACGC	4 cut(s)	317, 613, 879, 1207
CsiI	ACCWGGT	1 cut(s)	1588
Csp6I	GTAC	7 cut(s)	17, 421, 718, 850, 901, 1042, 1183
CspI	CGGWCCG	1 cut(s)	1091
CviQI	GTAC	7 cut(s)	17, 421, 718, 850, 901, 1042, 1183
DdeI	CTNAG	7 cut(s)	191, 414, 519, 759, 855, 1600, 1749
DpnI	GATC	2 cut(s)	461, 1391
DpnII	GATC	2 cut(s)	459, 1389
EaeI	YGGCCR	2 cut(s)	72, 582
Eam1104I	CTCTTC	2 cut(s)	1122, 1415
EarI	CTCTTC	2 cut(s)	1122, 1415
EciI	GGCGGA	1 cut(s)	188
Eco130I	CCWWGG	6 cut(s)	390, 441, 610, 865, 1451, 1491
Eco147I	AGGCCT	1 cut(s)	389
Eco31I	GGTCTC	1 cut(s)	400
Eco32I	GATATC	2 cut(s)	33, 703
Eco47I	GGWCC	4 cut(s)	355, 542, 1091, 1586
Eco47III	AGCGCT	1 cut(s)	217
Eco57I	CTGAAG	1 cut(s)	1533
Eco72I	CACGTG	1 cut(s)	1234
Eco81I	CCTNAGG	2 cut(s)	519, 855
Eco88I	CYCGRG	1 cut(s)	1257
EcoNI	CCTNNNNNAGG	1 cut(s)	1465
EcoO109I	RGGNCCY	3 cut(s)	355, 542, 1586
EcoRII	CCWGG	4 cut(s)	103, 1336, 1588, 1732
EcoRV	GATATC	2 cut(s)	33, 703
EcoT14I	CCWWGG	6 cut(s)	390, 441, 610, 865, 1451, 1491
ErhI	CCWWGG	6 cut(s)	390, 441, 610, 865, 1451, 1491
FalI	AAGNNNNNCTT	2 cut(s)	1616, 1648
FaqI	GGGAC	4 cut(s)	606, 1285, 1544, 1599
FauI	CCCGC	3 cut(s)	806, 1114, 1314
Fnu4HI	GCNGC	9 cut(s)	214, 243, 369, 674, 811, 1027, 1473, 1630, 1662
FokI	GGATG	6 cut(s)	509, 593, 627, 742, 1253, 1265
Fsp4HI	GCNGC	9 cut(s)	214, 243, 369, 674, 811, 1027, 1473, 1630, 1662
FspBI	CTAG	1 cut(s)	1467
GlaI	GCGC	6 cut(s)	126, 217, 270, 439, 739, 783
GluI	GCNGC	9 cut(s)	214, 243, 369, 674, 811, 1027, 1473, 1630, 1662
GsaI	CCCAGC	1 cut(s)	738
GsuI	CTGGAG	1 cut(s)	1320
HaeII	RGCGCY	1 cut(s)	219
HaeIII	GGCC	5 cut(s)	74, 389, 584, 960, 1450
HapII	CCGG	9 cut(s)	169, 197, 483, 1005, 1089, 1094, 1128, 1397, 1638
HgaI	GACGC	4 cut(s)	317, 613, 879, 1207
HhaI	GCGC	6 cut(s)	127, 218, 271, 440, 740, 784
Hin1I	GRCGYC	3 cut(s)	309, 624, 1198
Hin6I	GCGC	6 cut(s)	125, 216, 269, 438, 738, 782
HinP1I	GCGC	6 cut(s)	125, 216, 269, 438, 738, 782
HincII	GTYRAC	1 cut(s)	307
HindII	GTYRAC	1 cut(s)	307
HindIII	AAGCTT	1 cut(s)	749
HinfI	GANTC	3 cut(s)	293, 327, 1074
HpaII	CCGG	9 cut(s)	169, 197, 483, 1005, 1089, 1094, 1128, 1397, 1638
Hpy166II	GTNNAC	7 cut(s)	142, 307, 423, 472, 901, 1042, 1488
Hpy188I	TCNGA	3 cut(s)	65, 529, 1508
Hpy188III	TCNNGA	8 cut(s)	331, 374, 457, 803, 1094, 1227, 1288, 1576
Hpy8I	GTNNAC	7 cut(s)	142, 307, 423, 472, 901, 1042, 1488
Hpy99I	CGWCG	1 cut(s)	1679
HpyAV	CCTTC	8 cut(s)	232, 289, 634, 886, 1042, 1333, 1508, 1570
HpyCH4III	ACNGT	8 cut(s)	476, 691, 746, 913, 1154, 1372, 1535, 1675
HpyCH4IV	ACGT	3 cut(s)	1198, 1233, 1744
HpyCH4V	TGCA	6 cut(s)	245, 263, 351, 492, 1386, 1488
HpyF10VI	GCNNNNNNNGC	8 cut(s)	133, 530, 845, 1106, 1128, 1472, 1601, 1638
HpyF3I	CTNAG	7 cut(s)	191, 414, 519, 759, 855, 1600, 1749
HpySE526I	ACGT	3 cut(s)	1198, 1233, 1744
Hsp92I	GRCGYC	3 cut(s)	309, 624, 1198
HspAI	GCGC	6 cut(s)	125, 216, 269, 438, 738, 782
Kpn2I	TCCGGA	1 cut(s)	1093
KpnI	GGTACC	1 cut(s)	721
Kzo9I	GATC	2 cut(s)	459, 1389
LguI	GCTCTTC	1 cut(s)	1122
LmnI	GCTCC	4 cut(s)	114, 379, 445, 1339
Lsp1109I	GCAGC	6 cut(s)	225, 229, 380, 822, 1459, 1648
LweI	GCATC	5 cut(s)	400, 720, 921, 1152, 1243
MabI	ACCWGGT	1 cut(s)	1588
MaeI	CTAG	1 cut(s)	1467
MaeII	ACGT	3 cut(s)	1198, 1233, 1744
MaeIII	GTNAC	2 cut(s)	476, 983
MalI	GATC	2 cut(s)	461, 1391
MbiI	CCGCTC	1 cut(s)	1334
MboI	GATC	2 cut(s)	459, 1389
MboII	GAAGA	6 cut(s)	109, 901, 1109, 1138, 1402, 1468
MhlI	GDGCHC	1 cut(s)	1490
MlsI	TGGCCA	2 cut(s)	74, 584
MluCI	AATT	2 cut(s)	1158, 1667
MluNI	TGGCCA	2 cut(s)	74, 584
MlyI	GAGTC	2 cut(s)	302, 336
Mox20I	TGGCCA	2 cut(s)	74, 584
MroI	TCCGGA	1 cut(s)	1093
MroXI	GAANNNNTTC	1 cut(s)	278
MscI	TGGCCA	2 cut(s)	74, 584
MseI	TTAA	2 cut(s)	831, 1280
MslI	CAYNNNNRTG	2 cut(s)	90, 944
Msp20I	TGGCCA	2 cut(s)	74, 584
MspA1I	CMGCKG	2 cut(s)	813, 1661
MspI	CCGG	9 cut(s)	169, 197, 483, 1005, 1089, 1094, 1128, 1397, 1638
MspR9I	CCNGG	6 cut(s)	105, 170, 197, 1338, 1590, 1734
Mva1269I	GAATGC	1 cut(s)	353
MvaI	CCWGG	4 cut(s)	105, 1338, 1590, 1734
MvnI	CGCG	1 cut(s)	438
MwoI	GCNNNNNNNGC	8 cut(s)	133, 530, 845, 1106, 1128, 1472, 1601, 1638
NciI	CCSGG	2 cut(s)	170, 197
NcoI	CCATGG	2 cut(s)	1451, 1491
NdeII	GATC	2 cut(s)	459, 1389
NlaIV	GGNNCC	8 cut(s)	116, 322, 544, 719, 961, 1030, 1587, 1594
NmuCI	GTSAC	1 cut(s)	983
NspI	RCATGY	3 cut(s)	492, 949, 1687
NspV	TTCGAA	2 cut(s)	1425, 1740
PaeR7I	CTCGAG	1 cut(s)	1257
PceI	AGGCCT	1 cut(s)	389
PciI	ACATGT	1 cut(s)	945
PciSI	GCTCTTC	1 cut(s)	1122
PcsI	WCGNNNNNNNCGW	2 cut(s)	840, 1368
PctI	GAATGC	1 cut(s)	353
PdmI	GAANNNNTTC	1 cut(s)	278
PfeI	GAWTC	1 cut(s)	1074
PflFI	GACNNNGTC	1 cut(s)	292
PflMI	CCANNNNNTGG	1 cut(s)	110
PkrI	GCNGC	9 cut(s)	215, 244, 370, 675, 812, 1028, 1474, 1631, 1663
PleI	GAGTC	2 cut(s)	301, 335
PmaCI	CACGTG	1 cut(s)	1234
PmlI	CACGTG	1 cut(s)	1234
PpsI	GAGTC	2 cut(s)	301, 335
Ppu21I	YACGTR	1 cut(s)	1234
PpuMI	RGGWCCY	3 cut(s)	355, 542, 1586
PscI	ACATGT	1 cut(s)	945
Psp5II	RGGWCCY	3 cut(s)	355, 542, 1586
Psp6I	CCWGG	4 cut(s)	103, 1336, 1588, 1732
PspCI	CACGTG	1 cut(s)	1234
PspFI	CCCAGC	1 cut(s)	734
PspGI	CCWGG	4 cut(s)	103, 1336, 1588, 1732
PspN4I	GGNNCC	8 cut(s)	116, 322, 544, 719, 961, 1030, 1587, 1594
PspPI	GGNCC	5 cut(s)	355, 542, 959, 1091, 1586
PspPPI	RGGWCCY	3 cut(s)	355, 542, 1586
PspXI	VCTCGAGB	1 cut(s)	1257
PstI	CTGCAG	2 cut(s)	265, 1388
PsyI	GACNNNGTC	1 cut(s)	292
RsaI	GTAC	7 cut(s)	18, 422, 719, 851, 902, 1043, 1184
RsaNI	GTAC	7 cut(s)	17, 421, 718, 850, 901, 1042, 1183
RseI	CAYNNNNRTG	2 cut(s)	90, 944
Rsr2I	CGGWCCG	1 cut(s)	1091
RsrII	CGGWCCG	1 cut(s)	1091
SapI	GCTCTTC	1 cut(s)	1122
SaqAI	TTAA	2 cut(s)	831, 1280
SatI	GCNGC	9 cut(s)	214, 243, 369, 674, 811, 1027, 1473, 1630, 1662
Sau3AI	GATC	2 cut(s)	459, 1389
Sau96I	GGNCC	5 cut(s)	355, 542, 959, 1091, 1586
ScaI	AGTACT	1 cut(s)	18
SchI	GAGTC	2 cut(s)	302, 336
ScrFI	CCNGG	6 cut(s)	105, 170, 197, 1338, 1590, 1734
SduI	GDGCHC	1 cut(s)	1490
SexAI	ACCWGGT	1 cut(s)	1588
SfaNI	GCATC	5 cut(s)	400, 720, 921, 1152, 1243
SfcI	CTRYAG	5 cut(s)	261, 383, 690, 1207, 1384
Sfr274I	CTCGAG	1 cut(s)	1257
SfuI	TTCGAA	2 cut(s)	1425, 1740
SgrAI	CRCCGGYG	1 cut(s)	1637
SinI	GGWCC	4 cut(s)	355, 542, 1091, 1586
SlaI	CTCGAG	1 cut(s)	1257
SmiMI	CAYNNNNRTG	2 cut(s)	90, 944
SmlI	CTYRAG	2 cut(s)	801, 1257
SmoI	CTYRAG	2 cut(s)	801, 1257
Sse9I	AATT	2 cut(s)	1158, 1667
SseBI	AGGCCT	1 cut(s)	389
SspI	AATATT	1 cut(s)	1192
SspMI	CTAG	1 cut(s)	1467
StuI	AGGCCT	1 cut(s)	389
StyD4I	CCNGG	6 cut(s)	103, 168, 195, 1336, 1588, 1732
StyI	CCWWGG	6 cut(s)	390, 441, 610, 865, 1451, 1491
TaaI	ACNGT	8 cut(s)	476, 691, 746, 913, 1154, 1372, 1535, 1675
TaiI	ACGT	3 cut(s)	1201, 1236, 1747
TaqI	TCGA	4 cut(s)	1258, 1425, 1677, 1740
TasI	AATT	2 cut(s)	1158, 1667
TatI	WGTACW	4 cut(s)	16, 420, 900, 1182
TauI	GCSGC	3 cut(s)	676, 1029, 1632
TfiI	GAWTC	1 cut(s)	1074
Tru1I	TTAA	2 cut(s)	831, 1280
Tru9I	TTAA	2 cut(s)	831, 1280
TscAI	CASTG	1 cut(s)	985
TseFI	GTSAC	1 cut(s)	983
TseI	GCWGC	6 cut(s)	213, 242, 368, 810, 1472, 1661
Tsp45I	GTSAC	1 cut(s)	983
TspDTI	ATGAA	8 cut(s)	146, 251, 377, 391, 551, 1202, 1315, 1468
TspGWI	ACGGA	1 cut(s)	1065
TspRI	CASTG	1 cut(s)	985
Tth111I	GACNNNGTC	1 cut(s)	292
Van91I	CCANNNNNTGG	1 cut(s)	110
VneI	GTGCAC	1 cut(s)	1486
VpaK11BI	GGWCC	4 cut(s)	355, 542, 1091, 1586
XagI	CCTNNNNNAGG	1 cut(s)	1465
XapI	RAATTY	1 cut(s)	1667
XceI	RCATGY	3 cut(s)	492, 949, 1687
XhoI	CTCGAG	1 cut(s)	1257
XmnI	GAANNNNTTC	1 cut(s)	278
XspI	CTAG	1 cut(s)	1467
ZraI	GACGTC	1 cut(s)	1199
ZrmI	AGTACT	1 cut(s)	18

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.