Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0003768

Physical Location & Seq

Genomic Coordinates Forward (+)

49848660 .. 49851089

Gene Structure 2430 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG277900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1851 bp

ATGGAAAAGAAGGGAAAGGCAATAGTAGGTGGAATTTCAATCATCCTTGTGGTTGGAGCAGTAGTTGGTTGTGTAGCTGTTGTTTCACATAATCGCAGCAAAGGCAAGGATAGGCTTTCGACGAGTTCCAAGGCCGTCAATGCAATGTGTGAACCAACTGATTACAAGGAAGAATGCATTGACAGCCTGGCTGACGCTGCCAAGAATCAAAGCGCCACCCCAAAAGAACTTATCCGAGCTGCCATTGATTACACAATCGATAAGGTGAAAGCGGCAATAGAGAAATCAGATAGCATTATCAAAGAAGCTAAAAATGTCAGTTCCACTGGGAAAATGGCCTTCAAGGATTGCAATGACTTGTTGGAATCTTCAATCGAAAGTCTTCAATATTCGCTTTTGACACTCGTTGATACTGAAATGCAAAACATGAATAATAAAGAGGCTGATCTTAAGAACTGGTTCAGTGCTGCGATATCATTTCAGGAATCATGCATTGATGGGATTCCATACGAAGACCTTAAGAACCAAATGAACGAGGCAATGCTCCATGCTTCACAACTGACCAGCAACGCCTTAGCAATTGTGTCTTCCCTTGCTCCCATCCTAGGCAAATTCAATATCTCCATGAAAACTGACAGTAAAAGTTCAACCAGTCGTCGTCTCCTGGATGTAGAAATGGAAACGGATGAACATGGCACTTTTCCAGCATGGTTCCCAGCTGCAGATAGGAAGCTATTCGCACACCACAACGAGAACAATAACAATAGCAGCAACAACAACAACAACGACGACGTCGACAACAACGACAACAACAACAACAACAACGGGAGAGTCGACAACGACGACAACAACAACAACGGGAGAGTCGACAACGACGACGACAACAACAACGGGAGAGTTACACCCAACGCAGTAGTGGCAAAAGATGGGAGTGGACAATACAAAACAATTGGTGAAGCTCTTGCTGCGTACCCAAAGGGATTCCAGGGAAGATATGTTATATATGTGAAAGCTGGGATTTACAATGAGTATGTTACTGTGGCCAAGAATCAGGTCAATGTGTACATGTATGGAGATGGACCCCGGAGGACCATGATCACAGGCAGTAAAAGCTTTGCTGATGGCATCACCACCCAGGACACTGCCACATTCATCGTAATGGGGAATGGGTTCATTGGTAGAGCAATGGGATTTCAGAACACAGCCGGCCCCGAAAAGCATCAGGCAGTGGCACTCCGAGTCCAGTCGGAAATGTCAGCCTTCTTCAACTGCAGAATGGACGCCTACCAAGACACATTGTACGTGCAAGCATATCGCCAATTTTATCGCAACTGTGTCATATCCGGTACCGTTGACTTCATTTTTGGTGACTCATCAACAGTCATCCAGAATAGTTTACTCATTGTGAGGAAGCCTATGGACGGCCAAAAGAACATTGTGACTGCTCATGGAAGAAAAGATAAGCGGGAAACCACTGGTCTGGTTATCCAGAACTGCAGGATAGTCCCAGAACAGGCTTTGTTCCCAATGAGATTCCAGATTCAGTCATACTTGGGGCGGCCGTGGAAGATGTACTCGAGAACAGTTATCATGGAGTCCGAATTTGGGGACTTTATCCAACCAGCCGGTTGGCTAGAATGGGAAGGGACTTTTGGACTAGACACACTGTATTATGCAGAGTATGCAAATCGGGGTCCGGGTGCTGTAACCACAGGAAGGGTAAAATGGAACGGATACCATGTGATTACGGACAGAAATGAGGCTCTTCAATTCACTCCCGGTAATTTTCTAACAGGAGACCAATGGTTGAAAGACGCGGGTATACCTTACTTCCTGGGTTTAAAAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

616

Amino Acids

68.29

Weight (kDa)

5.67

Isoelectric Point (pI)

26.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	45 - 194	2.2e-29	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	303 - 600	1.2e-135	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003768)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47030 AT2G47040 AT2G47050 AT3G62170 AT3G62180 AT3G62180
fragaria_vesca	FvH4_1g26360 FvH4_4g21500
malus_domestica	MD16G1136400.v1.1 MD17G1283800.v1.1
prunus_persica	Prupe.1G105400_v2.0.a1 Prupe.3G003700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0486131 RchiOBHm_Chr4g0427511
rosa_laevigata	RLG00000007232 RLG00000023101
rosa_multiflora	Rmu_sc0001006.1_g000002 Rmu_sc0009627.1_g000001 Rmu_sc0017895.1_g000001 Rmu_sc0017895.1_g000002
rosa_roxburghii	Rroxscaffold_5G00369150 Rroxscaffold_6G00396030
rosa_rugosa	Rorug03G0223700 Rorug04G0218200
rosa_samantha	Rh3AG273500 Rh3BG308600 Rh3CG307100 Rh3DG303400 Rh4AG274700 Rh4BG280500 Rh4CG295800 Rh4DG277900
rosa_wichuraiana	Rw3G024240 Rw4G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	795
Acc65I	GGTACC	1 cut(s)	1346
AccB1I	GGYRCC	1 cut(s)	1346
AccI	GTMKAC	4 cut(s)	795, 834, 867, 1822
AccII	CGCG	1 cut(s)	1817
AciI	CCGC	4 cut(s)	272, 1465, 1558, 1817
AcoI	YGGCCR	3 cut(s)	1041, 1423, 1559
AcsI	RAATTY	3 cut(s)	33, 611, 1601
AcyI	GRCGYC	2 cut(s)	792, 1281
AfaI	GTAC	5 cut(s)	971, 1064, 1301, 1348, 1574
AfiI	CCNNNNNNNGG	5 cut(s)	605, 1421, 1513, 1605, 1716
AflII	CTTAAG	2 cut(s)	449, 518
AflIII	ACRYGT	1 cut(s)	1065
AgsI	TTSAA	9 cut(s)	39, 343, 372, 386, 616, 648, 1267, 1769, 1810
AjnI	CCWGG	5 cut(s)	186, 663, 984, 1134, 1833
AjuI	GAANNNNNNNTTGG	2 cut(s)	1635, 1667
AluBI	AGCT	8 cut(s)	77, 239, 308, 719, 733, 959, 1013, 1113
AluI	AGCT	8 cut(s)	77, 239, 308, 719, 733, 959, 1013, 1113
Alw26I	GTCTC	2 cut(s)	665, 1791
Ama87I	CYCGRG	1 cut(s)	1576
AoxI	GGCC	6 cut(s)	132, 336, 1041, 1207, 1423, 1559
ApeKI	GCWGC	7 cut(s)	96, 197, 239, 467, 719, 768, 965
ApoI	RAATTY	3 cut(s)	33, 611, 1601
ArsI	GACNNNNNNTTYG	2 cut(s)	1346, 1378
Asp700I	GAANNNNTTC	4 cut(s)	381, 458, 734, 1169
Asp718I	GGTACC	1 cut(s)	1346
AspA2I	CCTAGG	1 cut(s)	604
AspLEI	GCGC	1 cut(s)	215
AspS9I	GGNCC	4 cut(s)	1079, 1089, 1208, 1694
AsuC2I	CCSGG	3 cut(s)	1084, 1698, 1779
AsuHPI	GGTGA	4 cut(s)	277, 965, 1120, 1379
AvaI	CYCGRG	1 cut(s)	1576
AvaII	GGWCC	3 cut(s)	1079, 1089, 1694
AvrII	CCTAGG	1 cut(s)	604
BalI	TGGCCA	1 cut(s)	1043
BanI	GGYRCC	1 cut(s)	1346
BbsI	GAAGAC	3 cut(s)	374, 519, 579
BbvI	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 952
BccI	CCATC	5 cut(s)	491, 608, 920, 1070, 1115
BceAI	ACGGC	3 cut(s)	119, 1438, 1546
BcgI	CGANNNNNNTGC	2 cut(s)	1295, 1329
BciT130I	CCWGG	5 cut(s)	188, 665, 986, 1136, 1835
BciVI	GTATCC	1 cut(s)	1727
BclI	TGATCA	1 cut(s)	1095
BcnI	CCSGG	3 cut(s)	1084, 1698, 1779
BcoDI	GTCTC	2 cut(s)	665, 1791
BfaI	CTAG	4 cut(s)	605, 1634, 1658, 1849
BfmI	CTRYAG	3 cut(s)	720, 1270, 1495
BfoI	RGCGCY	1 cut(s)	216
BfrI	CTTAAG	2 cut(s)	449, 518
BfuI	GTATCC	1 cut(s)	1727
BisI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 966, 1559
BlnI	CCTAGG	1 cut(s)	604
BlsI	GCNGC	9 cut(s)	98, 199, 241, 274, 469, 721, 770, 967, 1560
Bme1390I	CCNGG	8 cut(s)	188, 665, 986, 1084, 1136, 1698, 1779, 1835
Bme18I	GGWCC	3 cut(s)	1079, 1089, 1694
BmeT110I	CYCGRG	1 cut(s)	1576
BmgT120I	GGNCC	4 cut(s)	1079, 1089, 1208, 1694
BmiI	GGNNCC	5 cut(s)	713, 1081, 1210, 1348, 1695
BmrFI	CCNGG	8 cut(s)	188, 665, 986, 1084, 1136, 1698, 1779, 1835
BmrI	ACTGGG	1 cut(s)	336
BmsI	GCATC	2 cut(s)	1134, 1228
BmuI	ACTGGG	1 cut(s)	336
BpiI	GAAGAC	3 cut(s)	374, 519, 579
Bpu10I	CCTNAGC	1 cut(s)	574
BpuMI	CCSGG	3 cut(s)	1084, 1698, 1779
Bsa29I	ATCGAT	1 cut(s)	258
BsaAI	YACGTR	1 cut(s)	1303
BsaHI	GRCGYC	2 cut(s)	792, 1281
BsaI	GGTCTC	1 cut(s)	1791
BsaJI	CCNNGG	7 cut(s)	129, 604, 985, 1082, 1134, 1562, 1834
BsaWI	WCCGGW	1 cut(s)	1343
BsaXI	ACNNNNNCTCC	2 cut(s)	48, 78
Bsc4I	CCNNNNNNNGG	5 cut(s)	605, 1421, 1513, 1605, 1716
Bse118I	RCCGGY	2 cut(s)	1205, 1625
Bse1I	ACTGG	5 cut(s)	331, 461, 651, 1243, 1480
Bse3DI	GCAATG	4 cut(s)	150, 358, 546, 1191
BseBI	CCWGG	5 cut(s)	188, 665, 986, 1136, 1835
BseCI	ATCGAT	1 cut(s)	258
BseDI	CCNNGG	7 cut(s)	129, 604, 985, 1082, 1134, 1562, 1834
BseGI	GGATG	5 cut(s)	42, 600, 673, 691, 1383
BseLI	CCNNNNNNNGG	5 cut(s)	605, 1421, 1513, 1605, 1716
BseMI	GCAATG	4 cut(s)	150, 358, 546, 1191
BseNI	ACTGG	5 cut(s)	331, 461, 651, 1243, 1480
BseX3I	CGGCCG	1 cut(s)	1559
BseXI	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 952
BseYI	CCCAGC	2 cut(s)	715, 1013
Bsh1236I	CGCG	1 cut(s)	1817
Bsh1285I	CGRYCG	1 cut(s)	1562
BshFI	GGCC	6 cut(s)	134, 338, 1043, 1209, 1425, 1561
BshNI	GGYRCC	1 cut(s)	1346
BshVI	ATCGAT	1 cut(s)	258
BsiEI	CGRYCG	1 cut(s)	1562
BsiHKCI	CYCGRG	1 cut(s)	1576
BsiSI	CCGG	6 cut(s)	1084, 1206, 1344, 1626, 1697, 1779
BslFI	GGGAC	3 cut(s)	1490, 1622, 1660
BslI	CCNNNNNNNGG	5 cut(s)	605, 1421, 1513, 1605, 1716
BsmAI	GTCTC	2 cut(s)	665, 1791
BsmBI	CGTCTC	1 cut(s)	665
BsmFI	GGGAC	3 cut(s)	1490, 1622, 1660
BsmI	GAATGC	1 cut(s)	179
BsnI	GGCC	6 cut(s)	134, 338, 1043, 1209, 1425, 1561
Bso31I	GGTCTC	1 cut(s)	1791
BsoBI	CYCGRG	1 cut(s)	1576
Bsp1407I	TGTACA	1 cut(s)	1062
Bsp143I	GATC	2 cut(s)	445, 1095
BspACI	CCGC	4 cut(s)	272, 1465, 1558, 1817
BspANI	GGCC	6 cut(s)	134, 338, 1043, 1209, 1425, 1561
BspDI	ATCGAT	1 cut(s)	258
BspFNI	CGCG	1 cut(s)	1817
BspLI	GGNNCC	5 cut(s)	713, 1081, 1210, 1348, 1695
BspMAI	CTGCAG	3 cut(s)	724, 1274, 1499
BspQI	GCTCTTC	1 cut(s)	1770
BspT107I	GGYRCC	1 cut(s)	1346
BspTI	CTTAAG	2 cut(s)	449, 518
BspTNI	GGTCTC	1 cut(s)	1791
BsrDI	GCAATG	4 cut(s)	150, 358, 546, 1191
BsrFI	RCCGGY	2 cut(s)	1205, 1625
BsrGI	TGTACA	1 cut(s)	1062
BsrI	ACTGG	5 cut(s)	331, 461, 651, 1243, 1480
BssAI	RCCGGY	2 cut(s)	1205, 1625
BssECI	CCNNGG	7 cut(s)	129, 604, 985, 1082, 1134, 1562, 1834
BssMI	GATC	2 cut(s)	445, 1095
BssNAI	GTATAC	1 cut(s)	1823
BssNI	GRCGYC	2 cut(s)	792, 1281
BssT1I	CCWWGG	2 cut(s)	129, 604
Bst1107I	GTATAC	1 cut(s)	1823
Bst2UI	CCWGG	5 cut(s)	188, 665, 986, 1136, 1835
Bst4CI	ACNGT	7 cut(s)	638, 1039, 1334, 1351, 1381, 1585, 1668
Bst6I	CTCTTC	1 cut(s)	1770
BstACI	GRCGYC	2 cut(s)	792, 1281
BstAFI	CTTAAG	2 cut(s)	449, 518
BstAPI	GCANNNNNTGC	1 cut(s)	1682
BstAUI	TGTACA	1 cut(s)	1062
BstBAI	YACGTR	1 cut(s)	1303
BstC8I	GCNNGC	2 cut(s)	1207, 1308
BstDEI	CTNAG	1 cut(s)	574
BstDSI	CCRYGG	1 cut(s)	1562
BstF5I	GGATG	5 cut(s)	42, 600, 673, 691, 1383
BstFNI	CGCG	1 cut(s)	1817
BstH2I	RGCGCY	1 cut(s)	216
BstHHI	GCGC	1 cut(s)	215
BstKTI	GATC	2 cut(s)	448, 1098
BstMAI	GTCTC	2 cut(s)	665, 1791
BstMBI	GATC	2 cut(s)	445, 1095
BstMCI	CGRYCG	1 cut(s)	1562
BstMWI	GCNNNNNNNGC	8 cut(s)	102, 140, 183, 197, 917, 965, 1110, 1682
BstNI	CCWGG	5 cut(s)	188, 665, 986, 1136, 1835
BstNSI	RCATGY	1 cut(s)	1069
BstSCI	CCNGG	8 cut(s)	186, 663, 984, 1082, 1134, 1696, 1777, 1833
BstSFI	CTRYAG	3 cut(s)	720, 1270, 1495
BstUI	CGCG	1 cut(s)	1817
BstV1I	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 952
BstV2I	GAAGAC	3 cut(s)	374, 519, 579
BstXI	CCANNNNNNTGG	2 cut(s)	1480, 1629
BstZ17I	GTATAC	1 cut(s)	1823
BstZI	CGGCCG	1 cut(s)	1559
Bsu15I	ATCGAT	1 cut(s)	258
BsuI	GTATCC	1 cut(s)	1727
BsuRI	GGCC	6 cut(s)	134, 338, 1043, 1209, 1425, 1561
BsuTUI	ATCGAT	1 cut(s)	258
BtgI	CCRYGG	1 cut(s)	1562
BtsCI	GGATG	5 cut(s)	42, 600, 673, 691, 1383
BtsI	GCAGTG	2 cut(s)	1140, 1233
BtsIMutI	CAGTG	6 cut(s)	324, 469, 1140, 1233, 1473, 1664
Cac8I	GCNNGC	2 cut(s)	1207, 1308
CfoI	GCGC	1 cut(s)	215
Cfr10I	RCCGGY	2 cut(s)	1205, 1625
Cfr13I	GGNCC	4 cut(s)	1079, 1089, 1208, 1694
ClaI	ATCGAT	1 cut(s)	258
CseI	GACGC	3 cut(s)	203, 1289, 1823
Csp6I	GTAC	5 cut(s)	970, 1063, 1300, 1347, 1573
CviQI	GTAC	5 cut(s)	970, 1063, 1300, 1347, 1573
DdeI	CTNAG	1 cut(s)	574
DpnI	GATC	2 cut(s)	447, 1097
DpnII	GATC	2 cut(s)	445, 1095
DraI	TTTAAA	1 cut(s)	1842
EaeI	YGGCCR	3 cut(s)	1041, 1423, 1559
EagI	CGGCCG	1 cut(s)	1559
Eam1104I	CTCTTC	1 cut(s)	1770
EarI	CTCTTC	1 cut(s)	1770
EclXI	CGGCCG	1 cut(s)	1559
Eco130I	CCWWGG	2 cut(s)	129, 604
Eco31I	GGTCTC	1 cut(s)	1791
Eco32I	GATATC	1 cut(s)	474
Eco47I	GGWCC	3 cut(s)	1079, 1089, 1694
Eco52I	CGGCCG	1 cut(s)	1559
Eco88I	CYCGRG	1 cut(s)	1576
EcoRII	CCWGG	5 cut(s)	186, 663, 984, 1134, 1833
EcoRV	GATATC	1 cut(s)	474
EcoT14I	CCWWGG	2 cut(s)	129, 604
EcoT22I	ATGCAT	2 cut(s)	179, 494
ErhI	CCWWGG	2 cut(s)	129, 604
Esp3I	CGTCTC	1 cut(s)	665
FaqI	GGGAC	3 cut(s)	1490, 1622, 1660
FauI	CCCGC	2 cut(s)	1458, 1810
FbaI	TGATCA	1 cut(s)	1095
FblI	GTMKAC	4 cut(s)	795, 834, 867, 1822
Fnu4HI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 966, 1559
FokI	GGATG	5 cut(s)	29, 587, 680, 698, 1370
Fsp4HI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 966, 1559
FspBI	CTAG	4 cut(s)	605, 1634, 1658, 1849
GlaI	GCGC	1 cut(s)	214
GluI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 966, 1559
GsaI	CCCAGC	2 cut(s)	719, 1017
HaeII	RGCGCY	1 cut(s)	216
HaeIII	GGCC	6 cut(s)	134, 338, 1043, 1209, 1425, 1561
HapII	CCGG	6 cut(s)	1084, 1206, 1344, 1626, 1697, 1779
HgaI	GACGC	3 cut(s)	203, 1289, 1823
HhaI	GCGC	1 cut(s)	215
Hin1I	GRCGYC	2 cut(s)	792, 1281
Hin6I	GCGC	1 cut(s)	213
HinP1I	GCGC	1 cut(s)	213
HincII	GTYRAC	4 cut(s)	796, 835, 868, 1354
HindII	GTYRAC	4 cut(s)	796, 835, 868, 1354
HindIII	AAGCTT	1 cut(s)	1111
HpaII	CCGG	6 cut(s)	1084, 1206, 1344, 1626, 1697, 1779
HphI	GGTGA	4 cut(s)	277, 965, 1120, 1379
Hpy166II	GTNNAC	9 cut(s)	152, 796, 835, 868, 935, 1063, 1354, 1397, 1823
Hpy188I	TCNGA	6 cut(s)	236, 289, 1197, 1238, 1249, 1600
Hpy188III	TCNNGA	5 cut(s)	482, 1387, 1489, 1537, 1578
Hpy8I	GTNNAC	9 cut(s)	152, 796, 835, 868, 935, 1063, 1354, 1397, 1823
Hpy99I	CGWCG	8 cut(s)	124, 660, 791, 794, 797, 845, 878, 881
HpyAV	CCTTC	5 cut(s)	4, 349, 1270, 1637, 1710
HpyCH4III	ACNGT	7 cut(s)	638, 1039, 1334, 1351, 1381, 1585, 1668
HpyCH4IV	ACGT	2 cut(s)	792, 1302
HpyF10VI	GCNNNNNNNGC	8 cut(s)	102, 140, 183, 197, 917, 965, 1110, 1682
HpyF3I	CTNAG	1 cut(s)	574
HpySE526I	ACGT	2 cut(s)	792, 1302
Hsp92I	GRCGYC	2 cut(s)	792, 1281
HspAI	GCGC	1 cut(s)	213
KpnI	GGTACC	1 cut(s)	1350
KroI	GCCGGC	1 cut(s)	1205
KroNI	GCCGGC	1 cut(s)	1207
Ksp22I	TGATCA	1 cut(s)	1095
Kzo9I	GATC	2 cut(s)	445, 1095
LguI	GCTCTTC	1 cut(s)	1770
LmnI	GCTCC	3 cut(s)	56, 549, 601
Lsp1109I	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 952
LweI	GCATC	2 cut(s)	1134, 1228
MaeI	CTAG	4 cut(s)	605, 1634, 1658, 1849
MaeII	ACGT	2 cut(s)	792, 1302
MaeIII	GTNAC	5 cut(s)	898, 1033, 1367, 1438, 1705
MalI	GATC	2 cut(s)	447, 1097
MboI	GATC	2 cut(s)	445, 1095
MfeI	CAATTG	2 cut(s)	579, 948
MlsI	TGGCCA	1 cut(s)	1043
MluCI	AATT	8 cut(s)	33, 579, 611, 948, 1319, 1601, 1769, 1783
MluNI	TGGCCA	1 cut(s)	1043
MlyI	GAGTC	5 cut(s)	840, 873, 1248, 1364, 1604
MmeI	TCCRAC	4 cut(s)	34, 342, 1227, 1642
MnlI	CCTC	5 cut(s)	433, 529, 1080, 1401, 1753
Mox20I	TGGCCA	1 cut(s)	1043
Mph1103I	ATGCAT	2 cut(s)	179, 494
MroNI	GCCGGC	1 cut(s)	1205
MroXI	GAANNNNTTC	4 cut(s)	381, 458, 734, 1169
MscI	TGGCCA	1 cut(s)	1043
MseI	TTAA	3 cut(s)	450, 519, 1841
MslI	CAYNNNNRTG	2 cut(s)	47, 1157
Msp20I	TGGCCA	1 cut(s)	1043
MspA1I	CMGCKG	1 cut(s)	719
MspCI	CTTAAG	2 cut(s)	449, 518
MspI	CCGG	6 cut(s)	1084, 1206, 1344, 1626, 1697, 1779
MspR9I	CCNGG	8 cut(s)	188, 665, 986, 1084, 1136, 1698, 1779, 1835
MunI	CAATTG	2 cut(s)	579, 948
Mva1269I	GAATGC	1 cut(s)	179
MvaI	CCWGG	5 cut(s)	188, 665, 986, 1136, 1835
MvnI	CGCG	1 cut(s)	1817
MwoI	GCNNNNNNNGC	8 cut(s)	102, 140, 183, 197, 917, 965, 1110, 1682
NaeI	GCCGGC	1 cut(s)	1207
NciI	CCSGG	3 cut(s)	1084, 1698, 1779
NdeII	GATC	2 cut(s)	445, 1095
NgoMIV	GCCGGC	1 cut(s)	1205
NlaIV	GGNNCC	5 cut(s)	713, 1081, 1210, 1348, 1695
NmuCI	GTSAC	2 cut(s)	1367, 1438
NsiI	ATGCAT	2 cut(s)	179, 494
NspI	RCATGY	1 cut(s)	1069
PaeR7I	CTCGAG	1 cut(s)	1576
PciI	ACATGT	1 cut(s)	1065
PciSI	GCTCTTC	1 cut(s)	1770
PcsI	WCGNNNNNNNCGW	6 cut(s)	792, 801, 831, 840, 864, 873
PctI	GAATGC	1 cut(s)	179
PdiI	GCCGGC	1 cut(s)	1207
PdmI	GAANNNNTTC	4 cut(s)	381, 458, 734, 1169
PfeI	GAWTC	8 cut(s)	205, 365, 485, 502, 981, 1048, 1533, 1540
PflFI	GACNNNGTC	1 cut(s)	791
PfoI	TCCNGGA	1 cut(s)	663
PkrI	GCNGC	9 cut(s)	98, 199, 241, 274, 469, 721, 770, 967, 1560
PleI	GAGTC	5 cut(s)	839, 872, 1247, 1364, 1603
PpsI	GAGTC	5 cut(s)	839, 872, 1247, 1364, 1603
Ppu21I	YACGTR	1 cut(s)	1303
PscI	ACATGT	1 cut(s)	1065
Psp6I	CCWGG	5 cut(s)	186, 663, 984, 1134, 1833
PspFI	CCCAGC	2 cut(s)	715, 1013
PspGI	CCWGG	5 cut(s)	186, 663, 984, 1134, 1833
PspN4I	GGNNCC	5 cut(s)	713, 1081, 1210, 1348, 1695
PspPI	GGNCC	4 cut(s)	1079, 1089, 1208, 1694
PstI	CTGCAG	3 cut(s)	724, 1274, 1499
PsyI	GACNNNGTC	1 cut(s)	791
PvuII	CAGCTG	1 cut(s)	719
RsaI	GTAC	5 cut(s)	971, 1064, 1301, 1348, 1574
RsaNI	GTAC	5 cut(s)	970, 1063, 1300, 1347, 1573
RseI	CAYNNNNRTG	2 cut(s)	47, 1157
SalI	GTCGAC	3 cut(s)	794, 833, 866
SapI	GCTCTTC	1 cut(s)	1770
SaqAI	TTAA	3 cut(s)	450, 519, 1841
SatI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 966, 1559
Sau3AI	GATC	2 cut(s)	445, 1095
Sau96I	GGNCC	4 cut(s)	1079, 1089, 1208, 1694
SchI	GAGTC	5 cut(s)	840, 873, 1248, 1364, 1604
ScrFI	CCNGG	8 cut(s)	188, 665, 986, 1084, 1136, 1698, 1779, 1835
SfaNI	GCATC	2 cut(s)	1134, 1228
SfcI	CTRYAG	3 cut(s)	720, 1270, 1495
Sfr274I	CTCGAG	1 cut(s)	1576
SinI	GGWCC	3 cut(s)	1079, 1089, 1694
SlaI	CTCGAG	1 cut(s)	1576
SmiMI	CAYNNNNRTG	2 cut(s)	47, 1157
SmlI	CTYRAG	3 cut(s)	449, 518, 1576
SmoI	CTYRAG	3 cut(s)	449, 518, 1576
Sse9I	AATT	8 cut(s)	33, 579, 611, 948, 1319, 1601, 1769, 1783
SsiI	CCGC	4 cut(s)	272, 1465, 1558, 1817
SspI	AATATT	1 cut(s)	389
SspMI	CTAG	4 cut(s)	605, 1634, 1658, 1849
StyD4I	CCNGG	8 cut(s)	186, 663, 984, 1082, 1134, 1696, 1777, 1833
StyI	CCWWGG	2 cut(s)	129, 604
TaaI	ACNGT	7 cut(s)	638, 1039, 1334, 1351, 1381, 1585, 1668
TaiI	ACGT	2 cut(s)	795, 1305
TaqI	TCGA	7 cut(s)	119, 258, 375, 795, 834, 867, 1577
TasI	AATT	8 cut(s)	33, 579, 611, 948, 1319, 1601, 1769, 1783
TatI	WGTACW	2 cut(s)	1062, 1572
TauI	GCSGC	2 cut(s)	275, 1561
TfiI	GAWTC	8 cut(s)	205, 365, 485, 502, 981, 1048, 1533, 1540
Tru1I	TTAA	3 cut(s)	450, 519, 1841
Tru9I	TTAA	3 cut(s)	450, 519, 1841
TscAI	CASTG	6 cut(s)	331, 469, 1147, 1233, 1480, 1671
TseFI	GTSAC	2 cut(s)	1367, 1438
TseI	GCWGC	7 cut(s)	96, 197, 239, 467, 719, 768, 965
Tsp45I	GTSAC	2 cut(s)	1367, 1438
TspDTI	ATGAA	7 cut(s)	443, 545, 641, 702, 1141, 1162, 1348
TspGWI	ACGGA	3 cut(s)	698, 1746, 1763
TspRI	CASTG	6 cut(s)	331, 469, 1147, 1233, 1480, 1671
Tth111I	GACNNNGTC	1 cut(s)	791
Vha464I	CTTAAG	2 cut(s)	449, 518
VpaK11BI	GGWCC	3 cut(s)	1079, 1089, 1694
XapI	RAATTY	3 cut(s)	33, 611, 1601
XceI	RCATGY	1 cut(s)	1069
XcmI	CCANNNNNNNNNTGG	2 cut(s)	331, 913
XhoI	CTCGAG	1 cut(s)	1576
XmaJI	CCTAGG	1 cut(s)	604
XmiI	GTMKAC	4 cut(s)	795, 834, 867, 1822
XmnI	GAANNNNTTC	4 cut(s)	381, 458, 734, 1169
XspI	CTAG	4 cut(s)	605, 1634, 1658, 1849
ZraI	GACGTC	1 cut(s)	793
Zsp2I	ATGCAT	2 cut(s)	179, 494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.