Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0003768

Physical Location & Seq

Genomic Coordinates Forward (+)

34280783 .. 34282525

Gene Structure 1743 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1283800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGGCACTTAATAAAAAAGTGGTAGTATCGGGGATTTCCCTGATACTTGTGGTTGGTGCGGTCATCGGAGCGGTGGTGGCCGTAGTCCACTACCAAAAGAAGCCGGGAAGCGAGTCTCTAACAGCGCAGCAGAAGATGGTGACCCAAATCTGTAATTTCACCGACTACCAACAGGAATGCCAGAAGAGCCTCGGCCCCGTCTCACAAAATAACAACGCCGAGTTTAAGGACTACGTAAAGGCCGCATTGATGTCCATCTCTGAGGAGGCCAACAAGGCCTTGAACACGTCCGGTTCACTTCTCGTCGACGCCAAAAATGGCACCTCCGCCAAGAGGACGCTCGAGGAGTGCAAGAACATGTTGAAGGAAGCTGTTGCCGAGGTGCAGGCGGTGTCCTCGTACGTGGGCGATGCTGACATGCACACCATGGAGGACCGCGTGATGGAGCTCAAGGCCTGGATTGGAAGCGTCATCAGTTATTCCACCACCTGCCTTGATATAATTGGCGAAAACGACCACAACCTCTACGAGGCATTGAAGGACCCCATCGGCAACGCCTCTGCCAGCACAGACAATGCATTGGCCATCGTTGGTGAGATCAGCAATGTCCTCGGCATGTTCGGTGTCAAAGTTAAGGACAAGGCCTTGAATGGCGGAGGAACTCAACGCCGGCTTTTGCAAAACGAGCTCAGCAGCGACGGGATCCCGACCTGGTTCTCGGCTTCCGACCGCAAGCTCATGGGGTTGCAAGACAACGGGAGGTTGAGGCCTCACATCGTTGTTGCCAAGGACGGGTCTGGCCAGTACAAGAGCATCAACGACGCGCTTAAGGCGTACCCGTACGGACAGAGAGGGCGATACATTATTTACGTCAAGGCCGGAGTTTACAATGAGTATGTTTACGTGGACAAGAAAATGATCAACGTCTACATGTACGGAGATGGACCCAGATCCACCGTCGTGACTGGAAGCAAGAGCGAAGCTGGAACCGGTGACAAAATCTCCACCAGTGGTACATTCCAAGTGTTTGGACCGGGTTTCATTGGAAGGTCCATGGGAATCATTAACACCGCCGGTCCAGAGGCAAGGCCAGCCGTTGCTCTCCGTACGGTCGGGGACCAAATCGCCTTCTTCAACTGCAGGATCAGCGGATACCAAGACACCTTGTACATGCAGTCCGGCCGACAGTTCTTCCGAAACTGTGTTATCTCCGGACACGTGGACTACATCTTCGGTGACGCCCGCGCATACATTCAGAACTCGTTGATCATCGTGAGGAAGTCAACCAACCCGCAGCAGAATCAAGCCTTCATCACCGCTCCCGGAAGGCAGTTCAAGCACGAGATCGGCGCCATCATCATCCACAACTGCAGGATCGTCCCCGAGCAGAAGCTCGTCCCCACGAGGTTCGAGGTGAGGACATACTTGGGAAGGCCGTGGAAGCCCTACTCCAGGGCTGTCTTCATGGAGAGCACTTTGGCAGACTTCATCAGGCCGGAGGGATACTCCGCTTGGGACGGCAAGGCCGGGAACACCCAACACGCCTTCTTCGGAGAGTTTAAGAACCGCGGCCCCGGTGCCAACCTTGCAAAGAGGGCCAGGTGGAGTGGTGACGGCTTCTACGGAGAGATGAACAGGGCCACCGCCGTGCAGTTCACAGCTCAACCCGCCCTGTTGGCTGATCGCTGGTTGAAATTTGCTAACATTCCGTACCTCCCCGGCTTCATTAGTGCTGATGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

581

Amino Acids

63.56

Weight (kDa)

9.12

Isoelectric Point (pI)

29.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	46 - 196	8.6e-19	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	258 - 556	5.5e-102	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003768)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47030 AT2G47040 AT2G47050 AT3G62170 AT3G62180 AT3G62180
fragaria_vesca	FvH4_1g26360 FvH4_4g21500
malus_domestica	MD16G1136400.v1.1 MD17G1283800.v1.1
prunus_persica	Prupe.1G105400_v2.0.a1 Prupe.3G003700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0486131 RchiOBHm_Chr4g0427511
rosa_laevigata	RLG00000007232 RLG00000023101
rosa_multiflora	Rmu_sc0001006.1_g000002 Rmu_sc0009627.1_g000001 Rmu_sc0017895.1_g000001 Rmu_sc0017895.1_g000002
rosa_roxburghii	Rroxscaffold_5G00369150 Rroxscaffold_6G00396030
rosa_rugosa	Rorug03G0223700 Rorug04G0218200
rosa_samantha	Rh3AG273500 Rh3BG308600 Rh3CG307100 Rh3DG303400 Rh4AG274700 Rh4BG280500 Rh4CG295800 Rh4DG277900
rosa_wichuraiana	Rw3G024240 Rw4G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	497
Acc36I	ACCTGC	1 cut(s)	497
AccB1I	GGYRCC	3 cut(s)	320, 1349, 1577
AccBSI	CCGCTC	2 cut(s)	71, 1319
AccI	GTMKAC	2 cut(s)	306, 927
AccII	CGCG	4 cut(s)	438, 824, 1245, 1569
AccIII	TCCGGA	1 cut(s)	1211
AclWI	GGATC	5 cut(s)	697, 710, 945, 1151, 1382
AcoI	YGGCCR	4 cut(s)	78, 582, 799, 1180
AcsI	RAATTY	1 cut(s)	1694
AcvI	CACGTG	1 cut(s)	1219
AcyI	GRCGYC	3 cut(s)	309, 1239, 1350
AfaI	GTAC	9 cut(s)	401, 806, 836, 842, 935, 1015, 1108, 1169, 1712
AfiI	CCNNNNNNNGG	5 cut(s)	333, 442, 529, 792, 1452
AflII	CTTAAG	1 cut(s)	827
AflIII	ACRYGT	4 cut(s)	285, 357, 930, 1216
AgeI	ACCGGT	1 cut(s)	989
AgsI	TTSAA	7 cut(s)	283, 364, 538, 649, 1135, 1336, 1693
AjiI	CACGTC	1 cut(s)	288
AjnI	CCWGG	4 cut(s)	455, 710, 1451, 1598
AleI	CACNNNNGTG	2 cut(s)	959, 1646
AluBI	AGCT	7 cut(s)	371, 448, 688, 736, 983, 1393, 1661
AluI	AGCT	7 cut(s)	371, 448, 688, 736, 983, 1393, 1661
Alw21I	GWGCWC	3 cut(s)	450, 690, 1475
Alw26I	GTCTC	2 cut(s)	120, 205
AlwI	GGATC	5 cut(s)	697, 710, 945, 1151, 1382
Ama87I	CYCGRG	2 cut(s)	341, 1382
Aor13HI	TCCGGA	1 cut(s)	1211
ApeKI	GCWGC	3 cut(s)	127, 693, 1294
ApoI	RAATTY	1 cut(s)	1694
ArsI	GACNNNNNNTTYG	2 cut(s)	1214, 1246
AsiGI	ACCGGT	1 cut(s)	989
AspLEI	GCGC	4 cut(s)	127, 826, 1247, 1352
AsuC2I	CCSGG	6 cut(s)	105, 1035, 1323, 1528, 1575, 1719
AsuHPI	GGTGA	8 cut(s)	151, 151, 605, 1004, 1247, 1306, 1426, 1622
AvaI	CYCGRG	2 cut(s)	341, 1382
AvaII	GGWCC	7 cut(s)	433, 541, 944, 1031, 1050, 1076, 1117
BalI	TGGCCA	2 cut(s)	584, 801
BamHI	GGATCC	1 cut(s)	702
BanI	GGYRCC	3 cut(s)	320, 1349, 1577
BanII	GRGCYC	2 cut(s)	450, 690
BauI	CACGAG	2 cut(s)	1340, 1402
BbrPI	CACGTG	1 cut(s)	1219
BbsI	GAAGAC	1 cut(s)	1453
Bbv12I	GWGCWC	3 cut(s)	450, 690, 1475
BbvI	GCAGC	3 cut(s)	139, 705, 1306
BccI	CCATC	7 cut(s)	130, 263, 436, 554, 593, 935, 1361
BceAI	ACGGC	6 cut(s)	65, 1079, 1420, 1534, 1630, 1631
BcgI	CGANNNNNNTGC	2 cut(s)	592, 626
BciT130I	CCWGG	4 cut(s)	457, 712, 1453, 1600
BciVI	GTATCC	2 cut(s)	1145, 1496
BclI	TGATCA	2 cut(s)	918, 1266
BcnI	CCSGG	6 cut(s)	105, 1035, 1323, 1528, 1575, 1719
BcoDI	GTCTC	2 cut(s)	120, 205
BfmI	CTRYAG	2 cut(s)	1138, 1369
BfoI	RGCGCY	1 cut(s)	1353
BfrI	CTTAAG	1 cut(s)	827
BfuAI	ACCTGC	1 cut(s)	497
BfuI	GTATCC	2 cut(s)	1145, 1496
BglI	GCCNNNNNGGC	2 cut(s)	275, 1676
BisI	GCNGC	5 cut(s)	128, 243, 694, 1295, 1570
BlpI	GCTNAGC	1 cut(s)	689
BlsI	GCNGC	5 cut(s)	129, 244, 695, 1296, 1571
Bme18I	GGWCC	7 cut(s)	433, 541, 944, 1031, 1050, 1076, 1117
BmeT110I	CYCGRG	2 cut(s)	341, 1382
BmgBI	CACGTC	1 cut(s)	288
BmsI	GCATC	3 cut(s)	400, 822, 1726
BpiI	GAAGAC	1 cut(s)	1453
BplI	GAGNNNNNCTC	2 cut(s)	1490, 1522
BpmI	CTGGAG	1 cut(s)	1435
Bpu1102I	GCTNAGC	1 cut(s)	689
BpuEI	CTTGAG	1 cut(s)	434
BpuMI	CCSGG	6 cut(s)	105, 1035, 1323, 1528, 1575, 1719
BsaAI	YACGTR	4 cut(s)	235, 403, 904, 1219
BsaBI	GATNNNNATC	1 cut(s)	254
BsaHI	GRCGYC	3 cut(s)	309, 1239, 1350
BsaWI	WCCGGW	3 cut(s)	290, 989, 1211
BsaXI	ACNNNNNCTCC	4 cut(s)	60, 90, 986, 1016
Bsc4I	CCNNNNNNNGG	5 cut(s)	333, 442, 529, 792, 1452
Bse118I	RCCGGY	3 cut(s)	669, 989, 1073
Bse1I	ACTGG	3 cut(s)	802, 970, 1008
Bse3DI	GCAATG	1 cut(s)	610
Bse8I	GATNNNNATC	1 cut(s)	254
BseAI	TCCGGA	1 cut(s)	1211
BseBI	CCWGG	4 cut(s)	457, 712, 1453, 1600
BseGI	GGATG	1 cut(s)	1359
BseJI	GATNNNNATC	1 cut(s)	254
BseLI	CCNNNNNNNGG	5 cut(s)	333, 442, 529, 792, 1452
BseMI	GCAATG	1 cut(s)	610
BseMII	CTCAG	2 cut(s)	252, 703
BseNI	ACTGG	3 cut(s)	802, 970, 1008
BseRI	GAGGAG	2 cut(s)	278, 359
BseX3I	CGGCCG	1 cut(s)	1180
BseXI	GCAGC	3 cut(s)	139, 705, 1306
BsgI	GTGCAG	2 cut(s)	404, 1670
Bsh1236I	CGCG	4 cut(s)	438, 824, 1245, 1569
Bsh1285I	CGRYCG	3 cut(s)	730, 1113, 1183
BshNI	GGYRCC	3 cut(s)	320, 1349, 1577
BshTI	ACCGGT	1 cut(s)	989
BsiEI	CGRYCG	3 cut(s)	730, 1113, 1183
BsiHKAI	GWGCWC	3 cut(s)	450, 690, 1475
BsiHKCI	CYCGRG	2 cut(s)	341, 1382
BsiWI	CGTACG	3 cut(s)	399, 840, 1106
BslFI	GGGAC	4 cut(s)	1130, 1364, 1382, 1529
BslI	CCNNNNNNNGG	5 cut(s)	333, 442, 529, 792, 1452
BsmAI	GTCTC	2 cut(s)	120, 205
BsmBI	CGTCTC	1 cut(s)	205
BsmFI	GGGAC	4 cut(s)	1130, 1364, 1382, 1529
BsmI	GAATGC	1 cut(s)	182
BsoBI	CYCGRG	2 cut(s)	341, 1382
Bsp1286I	GDGCHC	3 cut(s)	450, 690, 1475
Bsp13I	TCCGGA	1 cut(s)	1211
Bsp1407I	TGTACA	1 cut(s)	1167
Bsp143I	GATC	9 cut(s)	597, 702, 918, 950, 1143, 1266, 1344, 1374, 1681
Bsp1720I	GCTNAGC	1 cut(s)	689
Bsp19I	CCATGG	2 cut(s)	426, 1053
BspCNI	CTCAG	2 cut(s)	253, 702
BspEI	TCCGGA	1 cut(s)	1211
BspFNI	CGCG	4 cut(s)	438, 824, 1245, 1569
BspMAI	CTGCAG	2 cut(s)	1142, 1373
BspMI	ACCTGC	1 cut(s)	497
BspPI	GGATC	5 cut(s)	697, 710, 945, 1151, 1382
BspQI	GCTCTTC	1 cut(s)	179
BspT107I	GGYRCC	3 cut(s)	320, 1349, 1577
BspTI	CTTAAG	1 cut(s)	827
BsrBI	CCGCTC	2 cut(s)	71, 1319
BsrDI	GCAATG	1 cut(s)	610
BsrFI	RCCGGY	3 cut(s)	669, 989, 1073
BsrGI	TGTACA	1 cut(s)	1167
BsrI	ACTGG	3 cut(s)	802, 970, 1008
BssAI	RCCGGY	3 cut(s)	669, 989, 1073
BssMI	GATC	9 cut(s)	597, 702, 918, 950, 1143, 1266, 1344, 1374, 1681
BssNI	GRCGYC	3 cut(s)	309, 1239, 1350
BssSI	CACGAG	2 cut(s)	1340, 1402
BssT1I	CCWWGG	3 cut(s)	426, 786, 1053
Bst2BI	CACGAG	2 cut(s)	1340, 1402
Bst2UI	CCWGG	4 cut(s)	457, 712, 1453, 1600
Bst4CI	ACNGT	4 cut(s)	958, 1111, 1188, 1202
Bst6I	CTCTTC	1 cut(s)	179
BstACI	GRCGYC	3 cut(s)	309, 1239, 1350
BstAFI	CTTAAG	1 cut(s)	827
BstAUI	TGTACA	1 cut(s)	1167
BstBAI	YACGTR	4 cut(s)	235, 403, 904, 1219
BstC8I	GCNNGC	6 cut(s)	387, 565, 671, 734, 1092, 1243
BstDEI	CTNAG	2 cut(s)	261, 689
BstDSI	CCRYGG	4 cut(s)	426, 1053, 1436, 1567
BstEII	GGTNACC	1 cut(s)	139
BstENI	CCTNNNNNAGG	2 cut(s)	527, 1450
BstF5I	GGATG	1 cut(s)	1359
BstFNI	CGCG	4 cut(s)	438, 824, 1245, 1569
BstH2I	RGCGCY	1 cut(s)	1353
BstHHI	GCGC	4 cut(s)	127, 826, 1247, 1352
BstKTI	GATC	9 cut(s)	600, 705, 921, 953, 1146, 1269, 1347, 1377, 1684
BstMAI	GTCTC	2 cut(s)	120, 205
BstMBI	GATC	9 cut(s)	597, 702, 918, 950, 1143, 1266, 1344, 1374, 1681
BstMCI	CGRYCG	3 cut(s)	730, 1113, 1183
BstNI	CCWGG	4 cut(s)	457, 712, 1453, 1600
BstNSI	RCATGY	5 cut(s)	361, 421, 619, 934, 1174
BstPI	GGTNACC	1 cut(s)	139
BstSFI	CTRYAG	2 cut(s)	1138, 1369
BstSNI	TACGTA	1 cut(s)	235
BstUI	CGCG	4 cut(s)	438, 824, 1245, 1569
BstV1I	GCAGC	3 cut(s)	139, 705, 1306
BstV2I	GAAGAC	1 cut(s)	1453
BstX2I	RGATCY	2 cut(s)	702, 950
BstXI	CCANNNNNNTGG	1 cut(s)	1028
BstYI	RGATCY	2 cut(s)	702, 950
BstZI	CGGCCG	1 cut(s)	1180
BsuI	GTATCC	2 cut(s)	1145, 1496
BtgI	CCRYGG	4 cut(s)	426, 1053, 1436, 1567
BtgZI	GCGATG	1 cut(s)	423
BtrI	CACGTC	1 cut(s)	288
BtsCI	GGATG	1 cut(s)	1359
BtsIMutI	CAGTG	1 cut(s)	1015
BveI	ACCTGC	1 cut(s)	497
Cac8I	GCNNGC	6 cut(s)	387, 565, 671, 734, 1092, 1243
CfoI	GCGC	4 cut(s)	127, 826, 1247, 1352
Cfr10I	RCCGGY	3 cut(s)	669, 989, 1073
Cfr42I	CCGCGG	1 cut(s)	1570
CseI	GACGC	5 cut(s)	317, 346, 457, 830, 1247
CsiI	ACCWGGT	1 cut(s)	710
Csp6I	GTAC	9 cut(s)	400, 805, 835, 841, 934, 1014, 1107, 1168, 1711
CspAI	ACCGGT	1 cut(s)	989
CspCI	CAANNNNNGTGG	2 cut(s)	475, 510
CviAII	CATG	9 cut(s)	358, 418, 427, 616, 739, 931, 1054, 1171, 1465
CviQI	GTAC	9 cut(s)	400, 805, 835, 841, 934, 1014, 1107, 1168, 1711
DdeI	CTNAG	2 cut(s)	261, 689
DinI	GGCGCC	1 cut(s)	1351
DpnI	GATC	9 cut(s)	599, 704, 920, 952, 1145, 1268, 1346, 1376, 1683
DpnII	GATC	9 cut(s)	597, 702, 918, 950, 1143, 1266, 1344, 1374, 1681
EaeI	YGGCCR	4 cut(s)	78, 582, 799, 1180
EagI	CGGCCG	1 cut(s)	1180
Eam1104I	CTCTTC	1 cut(s)	179
EarI	CTCTTC	1 cut(s)	179
EciI	GGCGGA	2 cut(s)	316, 669
Ecl136II	GAGCTC	2 cut(s)	448, 688
EclXI	CGGCCG	1 cut(s)	1180
Eco105I	TACGTA	1 cut(s)	235
Eco130I	CCWWGG	3 cut(s)	426, 786, 1053
Eco147I	AGGCCT	4 cut(s)	278, 455, 644, 769
Eco24I	GRGCYC	2 cut(s)	450, 690
Eco47I	GGWCC	7 cut(s)	433, 541, 944, 1031, 1050, 1076, 1117
Eco52I	CGGCCG	1 cut(s)	1180
Eco53kI	GAGCTC	2 cut(s)	448, 688
Eco72I	CACGTG	1 cut(s)	1219
Eco88I	CYCGRG	2 cut(s)	341, 1382
Eco91I	GGTNACC	1 cut(s)	139
EcoICRI	GAGCTC	2 cut(s)	448, 688
EcoNI	CCTNNNNNAGG	2 cut(s)	527, 1450
EcoO109I	RGGNCCY	1 cut(s)	541
EcoO65I	GGTNACC	1 cut(s)	139
EcoRII	CCWGG	4 cut(s)	455, 710, 1451, 1598
EcoT14I	CCWWGG	3 cut(s)	426, 786, 1053
EcoT22I	ATGCAT	1 cut(s)	580
EcoT38I	GRGCYC	2 cut(s)	450, 690
EgeI	GGCGCC	1 cut(s)	1351
EheI	GGCGCC	1 cut(s)	1351
ErhI	CCWWGG	3 cut(s)	426, 786, 1053
Esp3I	CGTCTC	1 cut(s)	205
FaeI	CATG	9 cut(s)	361, 421, 430, 619, 742, 934, 1057, 1174, 1468
FaqI	GGGAC	4 cut(s)	1130, 1364, 1382, 1529
FatI	CATG	9 cut(s)	357, 417, 426, 615, 738, 930, 1053, 1170, 1464
FauI	CCCGC	3 cut(s)	1250, 1299, 1675
FbaI	TGATCA	2 cut(s)	918, 1266
FblI	GTMKAC	2 cut(s)	306, 927
Fnu4HI	GCNGC	5 cut(s)	128, 243, 694, 1295, 1570
FokI	GGATG	1 cut(s)	1346
FriOI	GRGCYC	2 cut(s)	450, 690
Fsp4HI	GCNGC	5 cut(s)	128, 243, 694, 1295, 1570
GlaI	GCGC	4 cut(s)	126, 825, 1246, 1351
GluI	GCNGC	5 cut(s)	128, 243, 694, 1295, 1570
GsuI	CTGGAG	1 cut(s)	1435
HaeII	RGCGCY	1 cut(s)	1353
HgaI	GACGC	5 cut(s)	317, 346, 457, 830, 1247
HhaI	GCGC	4 cut(s)	127, 826, 1247, 1352
Hin1I	GRCGYC	3 cut(s)	309, 1239, 1350
Hin1II	CATG	9 cut(s)	361, 421, 430, 619, 742, 934, 1057, 1174, 1468
Hin6I	GCGC	4 cut(s)	125, 824, 1245, 1350
HinP1I	GCGC	4 cut(s)	125, 824, 1245, 1350
HincII	GTYRAC	2 cut(s)	307, 1284
HindII	GTYRAC	2 cut(s)	307, 1284
HinfI	GANTC	3 cut(s)	113, 1059, 1300
HphI	GGTGA	8 cut(s)	151, 151, 605, 1004, 1247, 1306, 1426, 1622
Hpy188I	TCNGA	6 cut(s)	68, 262, 727, 1196, 1257, 1553
Hpy188III	TCNNGA	5 cut(s)	706, 961, 1079, 1212, 1273
Hpy99I	CGWCG	5 cut(s)	308, 311, 701, 824, 962
HpyAV	CCTTC	8 cut(s)	358, 532, 1041, 1138, 1318, 1320, 1425, 1555
HpyCH4III	ACNGT	4 cut(s)	958, 1111, 1188, 1202
HpyCH4IV	ACGT	7 cut(s)	234, 287, 402, 870, 903, 924, 1218
HpyF3I	CTNAG	2 cut(s)	261, 689
HpySE526I	ACGT	7 cut(s)	234, 287, 402, 870, 903, 924, 1218
Hsp92I	GRCGYC	3 cut(s)	309, 1239, 1350
Hsp92II	CATG	9 cut(s)	361, 421, 430, 619, 742, 934, 1057, 1174, 1468
HspAI	GCGC	4 cut(s)	125, 824, 1245, 1350
KasI	GGCGCC	1 cut(s)	1349
Kpn2I	TCCGGA	1 cut(s)	1211
KroI	GCCGGC	1 cut(s)	669
KroNI	GCCGGC	1 cut(s)	671
Ksp22I	TGATCA	2 cut(s)	918, 1266
KspI	CCGCGG	1 cut(s)	1570
Kzo9I	GATC	9 cut(s)	597, 702, 918, 950, 1143, 1266, 1344, 1374, 1681
LguI	GCTCTTC	1 cut(s)	179
LmnI	GCTCC	3 cut(s)	68, 445, 1324
Lsp1109I	GCAGC	3 cut(s)	139, 705, 1306
LweI	GCATC	3 cut(s)	400, 822, 1726
MabI	ACCWGGT	1 cut(s)	710
MaeII	ACGT	7 cut(s)	234, 287, 402, 870, 903, 924, 1218
MaeIII	GTNAC	5 cut(s)	139, 961, 992, 1235, 1610
MalI	GATC	9 cut(s)	599, 704, 920, 952, 1145, 1268, 1346, 1376, 1683
MbiI	CCGCTC	2 cut(s)	71, 1319
MboI	GATC	9 cut(s)	597, 702, 918, 950, 1143, 1266, 1344, 1374, 1681
MboII	GAAGA	7 cut(s)	145, 196, 1123, 1183, 1222, 1453, 1540
MflI	RGATCY	2 cut(s)	702, 950
MhlI	GDGCHC	3 cut(s)	450, 690, 1475
MlsI	TGGCCA	2 cut(s)	584, 801
MluCI	AATT	3 cut(s)	154, 501, 1694
MluNI	TGGCCA	2 cut(s)	584, 801
Mly113I	GGCGCC	1 cut(s)	1350
MlyI	GAGTC	1 cut(s)	122
MmeI	TCCRAC	1 cut(s)	750
Mox20I	TGGCCA	2 cut(s)	584, 801
Mph1103I	ATGCAT	1 cut(s)	580
MroI	TCCGGA	1 cut(s)	1211
MroNI	GCCGGC	1 cut(s)	669
MscI	TGGCCA	2 cut(s)	584, 801
MseI	TTAA	6 cut(s)	9, 225, 633, 828, 1065, 1560
MslI	CAYNNNNRTG	2 cut(s)	959, 1646
Msp20I	TGGCCA	2 cut(s)	584, 801
MspA1I	CMGCKG	2 cut(s)	1149, 1569
MspCI	CTTAAG	1 cut(s)	827
Mva1269I	GAATGC	1 cut(s)	182
MvaI	CCWGG	4 cut(s)	457, 712, 1453, 1600
MvnI	CGCG	4 cut(s)	438, 824, 1245, 1569
NaeI	GCCGGC	1 cut(s)	671
NarI	GGCGCC	1 cut(s)	1350
NciI	CCSGG	6 cut(s)	105, 1035, 1323, 1528, 1575, 1719
NcoI	CCATGG	2 cut(s)	426, 1053
NdeII	GATC	9 cut(s)	597, 702, 918, 950, 1143, 1266, 1344, 1374, 1681
NgoMIV	GCCGGC	1 cut(s)	669
NlaIII	CATG	9 cut(s)	361, 421, 430, 619, 742, 934, 1057, 1174, 1468
NmeAIII	GCCGAG	5 cut(s)	171, 244, 403, 591, 698
NmuCI	GTSAC	5 cut(s)	139, 961, 992, 1235, 1610
NsiI	ATGCAT	1 cut(s)	580
NspI	RCATGY	5 cut(s)	361, 421, 619, 934, 1174
OliI	CACNNNNGTG	2 cut(s)	959, 1646
PaeR7I	CTCGAG	1 cut(s)	341
PaqCI	CACCTGC	1 cut(s)	497
PceI	AGGCCT	4 cut(s)	278, 455, 644, 769
PciI	ACATGT	2 cut(s)	357, 930
PciSI	GCTCTTC	1 cut(s)	179
PctI	GAATGC	1 cut(s)	182
PdiI	GCCGGC	1 cut(s)	671
PfeI	GAWTC	2 cut(s)	1059, 1300
Pfl23II	CGTACG	3 cut(s)	399, 840, 1106
PfoI	TCCNGGA	1 cut(s)	1321
PinAI	ACCGGT	1 cut(s)	989
PkrI	GCNGC	5 cut(s)	129, 244, 695, 1296, 1571
PleI	GAGTC	1 cut(s)	121
PluTI	GGCGCC	1 cut(s)	1353
PmaCI	CACGTG	1 cut(s)	1219
PmlI	CACGTG	1 cut(s)	1219
PpsI	GAGTC	1 cut(s)	121
Ppu21I	YACGTR	4 cut(s)	235, 403, 904, 1219
PpuMI	RGGWCCY	1 cut(s)	541
PscI	ACATGT	2 cut(s)	357, 930
Psp124BI	GAGCTC	2 cut(s)	450, 690
Psp5II	RGGWCCY	1 cut(s)	541
Psp6I	CCWGG	4 cut(s)	455, 710, 1451, 1598
PspCI	CACGTG	1 cut(s)	1219
PspEI	GGTNACC	1 cut(s)	139
PspGI	CCWGG	4 cut(s)	455, 710, 1451, 1598
PspLI	CGTACG	3 cut(s)	399, 840, 1106
PspPPI	RGGWCCY	1 cut(s)	541
PspXI	VCTCGAGB	1 cut(s)	341
PstI	CTGCAG	2 cut(s)	1142, 1373
PsuI	RGATCY	2 cut(s)	702, 950
RsaI	GTAC	9 cut(s)	401, 806, 836, 842, 935, 1015, 1108, 1169, 1712
RsaNI	GTAC	9 cut(s)	400, 805, 835, 841, 934, 1014, 1107, 1168, 1711
RseI	CAYNNNNRTG	2 cut(s)	959, 1646
SacI	GAGCTC	2 cut(s)	450, 690
SacII	CCGCGG	1 cut(s)	1570
SalI	GTCGAC	1 cut(s)	305
SapI	GCTCTTC	1 cut(s)	179
SaqAI	TTAA	6 cut(s)	9, 225, 633, 828, 1065, 1560
SatI	GCNGC	5 cut(s)	128, 243, 694, 1295, 1570
Sau3AI	GATC	9 cut(s)	597, 702, 918, 950, 1143, 1266, 1344, 1374, 1681
SchI	GAGTC	1 cut(s)	122
SduI	GDGCHC	3 cut(s)	450, 690, 1475
SexAI	ACCWGGT	1 cut(s)	710
SfaNI	GCATC	3 cut(s)	400, 822, 1726
SfcI	CTRYAG	2 cut(s)	1138, 1369
SfiI	GGCCNNNNNGGCC	1 cut(s)	275
SfoI	GGCGCC	1 cut(s)	1351
Sfr274I	CTCGAG	1 cut(s)	341
Sfr303I	CCGCGG	1 cut(s)	1570
SgrBI	CCGCGG	1 cut(s)	1570
SgrDI	CGTCGACG	1 cut(s)	305
SinI	GGWCC	7 cut(s)	433, 541, 944, 1031, 1050, 1076, 1117
SlaI	CTCGAG	1 cut(s)	341
SmiMI	CAYNNNNRTG	2 cut(s)	959, 1646
SmlI	CTYRAG	3 cut(s)	341, 449, 827
SmoI	CTYRAG	3 cut(s)	341, 449, 827
SnaBI	TACGTA	1 cut(s)	235
Sse9I	AATT	3 cut(s)	154, 501, 1694
SseBI	AGGCCT	4 cut(s)	278, 455, 644, 769
SspDI	GGCGCC	1 cut(s)	1349
SstI	GAGCTC	2 cut(s)	450, 690
StuI	AGGCCT	4 cut(s)	278, 455, 644, 769
StyI	CCWWGG	3 cut(s)	426, 786, 1053
TaaI	ACNGT	4 cut(s)	958, 1111, 1188, 1202
TaiI	ACGT	7 cut(s)	237, 290, 405, 873, 906, 927, 1221
TaqI	TCGA	3 cut(s)	306, 342, 1410
TasI	AATT	3 cut(s)	154, 501, 1694
TatI	WGTACW	2 cut(s)	804, 1167
TauI	GCSGC	2 cut(s)	245, 1572
TfiI	GAWTC	2 cut(s)	1059, 1300
Tru1I	TTAA	6 cut(s)	9, 225, 633, 828, 1065, 1560
Tru9I	TTAA	6 cut(s)	9, 225, 633, 828, 1065, 1560
TscAI	CASTG	1 cut(s)	1015
TseFI	GTSAC	5 cut(s)	139, 961, 992, 1235, 1610
TseI	GCWGC	3 cut(s)	127, 693, 1294
Tsp45I	GTSAC	5 cut(s)	139, 961, 992, 1235, 1610
TspDTI	ATGAA	6 cut(s)	1030, 1300, 1453, 1477, 1646, 1714
TspGWI	ACGGA	5 cut(s)	858, 951, 1094, 1638, 1698
TspRI	CASTG	1 cut(s)	1015
Vha464I	CTTAAG	1 cut(s)	827
VpaK11BI	GGWCC	7 cut(s)	433, 541, 944, 1031, 1050, 1076, 1117
XagI	CCTNNNNNAGG	2 cut(s)	527, 1450
XapI	RAATTY	1 cut(s)	1694
XceI	RCATGY	5 cut(s)	361, 421, 619, 934, 1174
XhoI	CTCGAG	1 cut(s)	341
XmiI	GTMKAC	2 cut(s)	306, 927
Zsp2I	ATGCAT	1 cut(s)	580

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.