Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003768

Physical Location & Seq

Genomic Coordinates Reverse (-)

10441940 .. 10443774

Gene Structure 1835 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1136400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1701 bp

ATGGAGAAGAGAGGAAAGCTGGTGGTAGGAGGAGTTTCCATCATCCTTATCGTCGGCGTGGTGATCGGCCTTGTAGCTGTAGTTTGCACAAAAAAGAGTAACAAGGACCAACTTTCCACAAGTTCTAAGGCCGTTGCTGCTATTTGTGATCCTACGGATTACAAAGAGGCATGCATCGACAGCCTTGGATCCGTCGCGAAAAATGATAGCGCCACGCCCAAAGACCTCATCCAGGCCGCCATTGATTCCACAATTAAAACCGTAAAGTCTGCGATAGAGAACTCTAACAGCATTGCCAAATCCTTTAAAGCGATCAACTCGACGGGTAAAATGGCGATGGACGATTGCAAGGACTTGTTGGAATCGGCAATTGCCGATCTCCAAGCCTCTTTTTCGTTCGTTGGGGATAGCAACATGCACACAATGCACGACAGAGAGGAAGAGCTGCGGAATTGGTTCAGCGCGGTGATATCGTTCCAACAATCGTGCCTTGACGGCATTCCTCAGGAGGAGCTCCAAAAGCTCATGAAAGATAGCCTTCTCAACGCCACACAGCTAACCAGCAATGCCTTGGCAATTGCCACATCAATTGCACAAATCTTAGAGAACTTCAACATCACATTGAACAAAGACTTAAGGCCAACGAGCAGCTCGCGGATCCTTGAGCAAGAAAACGATGGGCATGGCCAGTATCCTAGTTGGTTTCCGGCCGCGGACAGGCGGCTGTTGGCAAAACAAGACAATGGAGGGGTTAAACCTAATGCCGTAGTGGCGAAGGACGGGAGTGGACAGTTTAAGACCATTGGTGCTGCCCTTGCTGCATATCCAAAGGGGCTTCAAGGAAGATATATCATATATGTGAAGGCTGGGGTTTACAATGAGTATGTTACTGTCACCAAAAATCAGGTCAATGTGTTCATGTATGGAGACGGACCCCGGAAGACCATGGTCACCGGAAGTAAAAGCTTTGCCGATGGCATCACCACTCAGAACACCGCCACATTCGCTGCAATTGGAAACGGATTTCTAGCAAAGGGGATGGGGTTTCAGAACACGGCAGGCGCCCAGAAGCATCAGGCTGTGGCACTGCGAGTCCAGTCGGAGATGTCAGCCTTCTTCAACTGCAGAATGGACGCCTACCAAGACACGTTGTACGCGCAAACACATCGCCAATTCTATCGAAACTGTGTCATTTCTGGCACAGTTGACTTCATCTTTGGTGACTCGCCAACGCTGATCCAAAATTCTTTGATCATAGTGAGGAAGCCTATGGACAACCAAAAGAACACAGTGACGGCTCAGGGAAGAGAGGACAGGAGGGAAAACACCGCCATTGTCATCCAGAACTGCAGAATAGTCCCTGAGGAGGTTCTGTTCCCATTAAGGTTCAAGATTCCGTCATACTTAGGCAGGCCATGGAAGACGTATGCAAGGACGGTGATCATGGAGTCGGAGCTCGGAGACTTCATACAACCAGCAGGGTGGCTACCCTGGAGTGGCAACTTTGCACTCGATACTCTATATTACGCAGAGTATGGGAATCGGGGTCCGGGGGCTGCGACCAACCAAAGGGTGAGATGGAAGGGATATCATGTGATTAACAGAAATGACGCTGTCCAGTTCACCGCCGGTCCATTTCTATTGGCGGATCGGTGGTTGAAAACCACCGGCATACCGTACTTTGCCGGTTTGAAAACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

567

Amino Acids

62.13

Weight (kDa)

8.94

Isoelectric Point (pI)

35.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	44 - 193	1.3e-28	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	254 - 548	3.2e-134	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003768)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47030 AT2G47040 AT2G47050 AT3G62170 AT3G62180 AT3G62180
fragaria_vesca	FvH4_1g26360 FvH4_4g21500
malus_domestica	MD16G1136400.v1.1 MD17G1283800.v1.1
prunus_persica	Prupe.1G105400_v2.0.a1 Prupe.3G003700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0486131 RchiOBHm_Chr4g0427511
rosa_laevigata	RLG00000007232 RLG00000023101
rosa_multiflora	Rmu_sc0001006.1_g000002 Rmu_sc0009627.1_g000001 Rmu_sc0017895.1_g000001 Rmu_sc0017895.1_g000002
rosa_roxburghii	Rroxscaffold_5G00369150 Rroxscaffold_6G00396030
rosa_rugosa	Rorug03G0223700 Rorug04G0218200
rosa_samantha	Rh3AG273500 Rh3BG308600 Rh3CG307100 Rh3DG303400 Rh4AG274700 Rh4BG280500 Rh4CG295800 Rh4DG277900
rosa_wichuraiana	Rw3G024240 Rw4G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1061
AccII	CGCG	5 cut(s)	197, 464, 655, 713, 1157
AclWI	GGATC	7 cut(s)	143, 183, 196, 652, 665, 1231, 1656
AcoI	YGGCCR	2 cut(s)	685, 708
AcsI	RAATTY	1 cut(s)	1243
AcyI	GRCGYC	2 cut(s)	1062, 1134
AfaI	GTAC	2 cut(s)	1154, 1679
AfiI	CCNNNNNNNGG	5 cut(s)	232, 717, 1366, 1496, 1569
AflII	CTTAAG	1 cut(s)	634
AflIII	ACRYGT	1 cut(s)	1146
AgsI	TTSAA	7 cut(s)	613, 625, 839, 1120, 1390, 1660, 1693
AjnI	CCWGG	2 cut(s)	231, 1490
AloI	GAACNNNNNNTCC	2 cut(s)	1358, 1390
AluBI	AGCT	9 cut(s)	19, 77, 445, 514, 523, 556, 651, 966, 1456
AluI	AGCT	9 cut(s)	19, 77, 445, 514, 523, 556, 651, 966, 1456
Alw21I	GWGCWC	2 cut(s)	516, 1458
Alw26I	GTCTC	2 cut(s)	921, 1455
AlwI	GGATC	7 cut(s)	143, 183, 196, 652, 665, 1231, 1656
AoxI	GGCC	7 cut(s)	67, 129, 234, 638, 685, 708, 1412
ApeKI	GCWGC	7 cut(s)	137, 445, 648, 809, 818, 1007, 1556
ApoI	RAATTY	1 cut(s)	1243
ArsI	GACNNNNNNTTYG	4 cut(s)	891, 923, 1199, 1231
Asp700I	GAANNNNTTC	1 cut(s)	455
AspLEI	GCGC	4 cut(s)	212, 464, 1064, 1159
AspS9I	GGNCC	4 cut(s)	106, 932, 1547, 1631
AsuC2I	CCSGG	2 cut(s)	937, 1551
AsuHPI	GGTGA	9 cut(s)	73, 478, 886, 943, 973, 1232, 1450, 1585, 1615
AvaII	GGWCC	4 cut(s)	106, 932, 1547, 1631
AxyI	CCTNAGG	2 cut(s)	504, 1362
BalI	TGGCCA	1 cut(s)	687
BamHI	GGATCC	2 cut(s)	188, 657
BanI	GGYRCC	1 cut(s)	1061
BanII	GRGCYC	2 cut(s)	516, 1458
BbsI	GAAGAC	2 cut(s)	947, 1427
Bbv12I	GWGCWC	2 cut(s)	516, 1458
BbvI	GCAGC	7 cut(s)	124, 432, 660, 796, 805, 994, 1543
BccI	CCATC	6 cut(s)	47, 331, 671, 968, 1033, 1572
BceAI	ACGGC	5 cut(s)	116, 511, 749, 1071, 1311
BcgI	CGANNNNNNTGC	2 cut(s)	1148, 1182
BciT130I	CCWGG	2 cut(s)	233, 1492
BciVI	GTATCC	1 cut(s)	702
BclI	TGATCA	2 cut(s)	1251, 1440
BcnI	CCSGG	2 cut(s)	937, 1551
BcoDI	GTCTC	2 cut(s)	921, 1455
BfaI	CTAG	3 cut(s)	696, 1028, 1699
BfmI	CTRYAG	3 cut(s)	78, 1123, 1348
BfoI	RGCGCY	2 cut(s)	213, 1065
BfrI	CTTAAG	1 cut(s)	634
BfuI	GTATCC	1 cut(s)	702
BglI	GCCNNNNNGGC	2 cut(s)	495, 770
Bme1390I	CCNGG	4 cut(s)	233, 937, 1492, 1551
Bme18I	GGWCC	4 cut(s)	106, 932, 1547, 1631
BmgT120I	GGNCC	4 cut(s)	106, 932, 1547, 1631
BmiI	GGNNCC	5 cut(s)	190, 659, 934, 1063, 1548
BmrFI	CCNGG	4 cut(s)	233, 937, 1492, 1551
BmsI	GCATC	3 cut(s)	183, 987, 1081
BoxI	GACNNNNGTC	1 cut(s)	947
BpiI	GAAGAC	2 cut(s)	947, 1427
BpmI	CTGGAG	1 cut(s)	1513
Bpu10I	CCTNAGC	1 cut(s)	1299
BpuEI	CTTGAG	1 cut(s)	683
BpuMI	CCSGG	2 cut(s)	937, 1551
BsaHI	GRCGYC	2 cut(s)	1062, 1134
BsaJI	CCNNGG	8 cut(s)	184, 570, 711, 935, 945, 1415, 1490, 1550
BsaWI	WCCGGW	1 cut(s)	953
BsaXI	ACNNNNNCTCC	2 cut(s)	1358, 1388
Bsc4I	CCNNNNNNNGG	5 cut(s)	232, 717, 1366, 1496, 1569
Bse118I	RCCGGY	3 cut(s)	1628, 1667, 1685
Bse1I	ACTGG	3 cut(s)	688, 1096, 1618
Bse21I	CCTNAGG	2 cut(s)	504, 1362
Bse3DI	GCAATG	2 cut(s)	291, 571
BseBI	CCWGG	2 cut(s)	233, 1492
BseDI	CCNNGG	8 cut(s)	184, 570, 711, 935, 945, 1415, 1490, 1550
BseGI	GGATG	4 cut(s)	42, 228, 1044, 1338
BseLI	CCNNNNNNNGG	5 cut(s)	232, 717, 1366, 1496, 1569
BseMI	GCAATG	2 cut(s)	291, 571
BseMII	CTCAG	4 cut(s)	518, 1001, 1313, 1353
BseNI	ACTGG	3 cut(s)	688, 1096, 1618
BseRI	GAGGAG	3 cut(s)	45, 524, 1379
BseX3I	CGGCCG	1 cut(s)	708
BseXI	GCAGC	7 cut(s)	124, 432, 660, 796, 805, 994, 1543
BseYI	CCCAGC	1 cut(s)	866
Bsh1236I	CGCG	5 cut(s)	197, 464, 655, 713, 1157
Bsh1285I	CGRYCG	1 cut(s)	711
BshFI	GGCC	7 cut(s)	69, 131, 236, 640, 687, 710, 1414
BshNI	GGYRCC	1 cut(s)	1061
BsiEI	CGRYCG	1 cut(s)	711
BsiHKAI	GWGCWC	2 cut(s)	516, 1458
BsiSI	CCGG	7 cut(s)	707, 937, 954, 1550, 1629, 1668, 1686
BslFI	GGGAC	1 cut(s)	1343
BslI	CCNNNNNNNGG	5 cut(s)	232, 717, 1366, 1496, 1569
BsmAI	GTCTC	2 cut(s)	921, 1455
BsmBI	CGTCTC	1 cut(s)	921
BsmFI	GGGAC	1 cut(s)	1343
BsmI	GAATGC	1 cut(s)	498
BsnI	GGCC	7 cut(s)	69, 131, 236, 640, 687, 710, 1414
Bsp1286I	GDGCHC	2 cut(s)	516, 1458
Bsp19I	CCATGG	2 cut(s)	945, 1415
Bsp68I	TCGCGA	1 cut(s)	197
BspANI	GGCC	7 cut(s)	69, 131, 236, 640, 687, 710, 1414
BspCNI	CTCAG	4 cut(s)	517, 1000, 1312, 1354
BspFNI	CGCG	5 cut(s)	197, 464, 655, 713, 1157
BspHI	TCATGA	1 cut(s)	525
BspLI	GGNNCC	5 cut(s)	190, 659, 934, 1063, 1548
BspMAI	CTGCAG	2 cut(s)	1127, 1352
BspPI	GGATC	7 cut(s)	143, 183, 196, 652, 665, 1231, 1656
BspQI	GCTCTTC	1 cut(s)	435
BspT107I	GGYRCC	1 cut(s)	1061
BspTI	CTTAAG	1 cut(s)	634
BsrDI	GCAATG	2 cut(s)	291, 571
BsrFI	RCCGGY	3 cut(s)	1628, 1667, 1685
BsrI	ACTGG	3 cut(s)	688, 1096, 1618
BssAI	RCCGGY	3 cut(s)	1628, 1667, 1685
BssECI	CCNNGG	8 cut(s)	184, 570, 711, 935, 945, 1415, 1490, 1550
BssNI	GRCGYC	2 cut(s)	1062, 1134
BssT1I	CCWWGG	4 cut(s)	184, 570, 945, 1415
Bst2UI	CCWGG	2 cut(s)	233, 1492
Bst4CI	ACNGT	8 cut(s)	262, 792, 892, 1187, 1204, 1291, 1438, 1677
Bst6I	CTCTTC	3 cut(s)	2, 435, 1300
BstACI	GRCGYC	2 cut(s)	1062, 1134
BstAFI	CTTAAG	1 cut(s)	634
BstAPI	GCANNNNNTGC	1 cut(s)	424
BstC8I	GCNNGC	4 cut(s)	172, 653, 1060, 1412
BstDEI	CTNAG	7 cut(s)	126, 504, 601, 987, 1299, 1362, 1405
BstDSI	CCRYGG	3 cut(s)	711, 945, 1415
BstEII	GGTNACC	1 cut(s)	949
BstENI	CCTNNNNNAGG	1 cut(s)	230
BstF5I	GGATG	4 cut(s)	42, 228, 1044, 1338
BstFNI	CGCG	5 cut(s)	197, 464, 655, 713, 1157
BstH2I	RGCGCY	2 cut(s)	213, 1065
BstHHI	GCGC	4 cut(s)	212, 464, 1064, 1159
BstMAI	GTCTC	2 cut(s)	921, 1455
BstMCI	CGRYCG	1 cut(s)	711
BstMWI	GCNNNNNNNGC	9 cut(s)	137, 180, 424, 495, 520, 770, 815, 818, 1004
BstNI	CCWGG	2 cut(s)	233, 1492
BstNSI	RCATGY	2 cut(s)	174, 418
BstPAI	GACNNNNGTC	1 cut(s)	947
BstPI	GGTNACC	1 cut(s)	949
BstSCI	CCNGG	4 cut(s)	231, 935, 1490, 1549
BstSFI	CTRYAG	3 cut(s)	78, 1123, 1348
BstUI	CGCG	5 cut(s)	197, 464, 655, 713, 1157
BstV1I	GCAGC	7 cut(s)	124, 432, 660, 796, 805, 994, 1543
BstV2I	GAAGAC	2 cut(s)	947, 1427
BstX2I	RGATCY	2 cut(s)	188, 657
BstXI	CCANNNNNNTGG	1 cut(s)	1482
BstYI	RGATCY	2 cut(s)	188, 657
BstZI	CGGCCG	1 cut(s)	708
Bsu36I	CCTNAGG	2 cut(s)	504, 1362
BsuI	GTATCC	1 cut(s)	702
BsuRI	GGCC	7 cut(s)	69, 131, 236, 640, 687, 710, 1414
BtgI	CCRYGG	3 cut(s)	711, 945, 1415
BtgZI	GCGATG	2 cut(s)	350, 1151
BtsCI	GGATG	4 cut(s)	42, 228, 1044, 1338
BtsI	GCAGTG	1 cut(s)	1085
BtsIMutI	CAGTG	2 cut(s)	1085, 1296
BtuMI	TCGCGA	1 cut(s)	197
Cac8I	GCNNGC	4 cut(s)	172, 653, 1060, 1412
CciI	TCATGA	1 cut(s)	525
CfoI	GCGC	4 cut(s)	212, 464, 1064, 1159
Cfr10I	RCCGGY	3 cut(s)	1628, 1667, 1685
Cfr13I	GGNCC	4 cut(s)	106, 932, 1547, 1631
Cfr42I	CCGCGG	1 cut(s)	714
CseI	GACGC	2 cut(s)	1142, 1619
Csp6I	GTAC	2 cut(s)	1153, 1678
CspCI	CAANNNNNGTGG	2 cut(s)	571, 606
CviAII	CATG	9 cut(s)	171, 415, 526, 683, 919, 946, 1416, 1444, 1592
CviQI	GTAC	2 cut(s)	1153, 1678
DdeI	CTNAG	7 cut(s)	126, 504, 601, 987, 1299, 1362, 1405
DinI	GGCGCC	1 cut(s)	1063
DraI	TTTAAA	1 cut(s)	307
EaeI	YGGCCR	2 cut(s)	685, 708
EagI	CGGCCG	1 cut(s)	708
Eam1104I	CTCTTC	3 cut(s)	2, 435, 1300
EarI	CTCTTC	3 cut(s)	2, 435, 1300
EciI	GGCGGA	1 cut(s)	1661
Ecl136II	GAGCTC	2 cut(s)	514, 1456
EclXI	CGGCCG	1 cut(s)	708
Eco130I	CCWWGG	4 cut(s)	184, 570, 945, 1415
Eco24I	GRGCYC	2 cut(s)	516, 1458
Eco32I	GATATC	2 cut(s)	471, 1589
Eco47I	GGWCC	4 cut(s)	106, 932, 1547, 1631
Eco52I	CGGCCG	1 cut(s)	708
Eco53kI	GAGCTC	2 cut(s)	514, 1456
Eco81I	CCTNAGG	2 cut(s)	504, 1362
Eco91I	GGTNACC	1 cut(s)	949
EcoICRI	GAGCTC	2 cut(s)	514, 1456
EcoNI	CCTNNNNNAGG	1 cut(s)	230
EcoO65I	GGTNACC	1 cut(s)	949
EcoRII	CCWGG	2 cut(s)	231, 1490
EcoRV	GATATC	2 cut(s)	471, 1589
EcoT14I	CCWWGG	4 cut(s)	184, 570, 945, 1415
EcoT22I	ATGCAT	1 cut(s)	176
EcoT38I	GRGCYC	2 cut(s)	516, 1458
EgeI	GGCGCC	1 cut(s)	1063
EheI	GGCGCC	1 cut(s)	1063
ErhI	CCWWGG	4 cut(s)	184, 570, 945, 1415
Esp3I	CGTCTC	1 cut(s)	921
FaeI	CATG	9 cut(s)	174, 418, 529, 686, 922, 949, 1419, 1447, 1595
FalI	AAGNNNNNCTT	2 cut(s)	522, 554
FaqI	GGGAC	1 cut(s)	1343
FatI	CATG	9 cut(s)	170, 414, 525, 682, 918, 945, 1415, 1443, 1591
FbaI	TGATCA	2 cut(s)	1251, 1440
FokI	GGATG	4 cut(s)	29, 215, 1051, 1325
FriOI	GRGCYC	2 cut(s)	516, 1458
FspBI	CTAG	3 cut(s)	696, 1028, 1699
GlaI	GCGC	4 cut(s)	211, 463, 1063, 1158
GsaI	CCCAGC	1 cut(s)	870
GsuI	CTGGAG	1 cut(s)	1513
HaeII	RGCGCY	2 cut(s)	213, 1065
HaeIII	GGCC	7 cut(s)	69, 131, 236, 640, 687, 710, 1414
HapII	CCGG	7 cut(s)	707, 937, 954, 1550, 1629, 1668, 1686
HgaI	GACGC	2 cut(s)	1142, 1619
HhaI	GCGC	4 cut(s)	212, 464, 1064, 1159
Hin1I	GRCGYC	2 cut(s)	1062, 1134
Hin1II	CATG	9 cut(s)	174, 418, 529, 686, 922, 949, 1419, 1447, 1595
Hin6I	GCGC	4 cut(s)	210, 462, 1062, 1157
HinP1I	GCGC	4 cut(s)	210, 462, 1062, 1157
HincII	GTYRAC	1 cut(s)	1207
HindII	GTYRAC	1 cut(s)	1207
HindIII	AAGCTT	1 cut(s)	964
HinfI	GANTC	7 cut(s)	245, 362, 1092, 1223, 1393, 1448, 1540
HpaII	CCGG	7 cut(s)	707, 937, 954, 1550, 1629, 1668, 1686
HphI	GGTGA	9 cut(s)	73, 478, 886, 943, 973, 1232, 1450, 1585, 1615
Hpy166II	GTNNAC	4 cut(s)	788, 874, 1207, 1623
Hpy188I	TCNGA	5 cut(s)	990, 1050, 1102, 1453, 1460
Hpy188III	TCNNGA	5 cut(s)	196, 506, 526, 1342, 1390
Hpy8I	GTNNAC	4 cut(s)	788, 874, 1207, 1623
Hpy99I	CGWCG	3 cut(s)	56, 197, 325
HpyAV	CCTTC	5 cut(s)	548, 769, 856, 1123, 1576
HpyCH4III	ACNGT	8 cut(s)	262, 792, 892, 1187, 1204, 1291, 1438, 1677
HpyCH4IV	ACGT	2 cut(s)	1148, 1424
HpyF10VI	GCNNNNNNNGC	9 cut(s)	137, 180, 424, 495, 520, 770, 815, 818, 1004
HpyF3I	CTNAG	7 cut(s)	126, 504, 601, 987, 1299, 1362, 1405
HpySE526I	ACGT	2 cut(s)	1148, 1424
Hsp92I	GRCGYC	2 cut(s)	1062, 1134
Hsp92II	CATG	9 cut(s)	174, 418, 529, 686, 922, 949, 1419, 1447, 1595
HspAI	GCGC	4 cut(s)	210, 462, 1062, 1157
KasI	GGCGCC	1 cut(s)	1061
Ksp22I	TGATCA	2 cut(s)	1251, 1440
KspI	CCGCGG	1 cut(s)	714
LguI	GCTCTTC	1 cut(s)	435
LmnI	GCTCC	3 cut(s)	511, 519, 1453
Lsp1109I	GCAGC	7 cut(s)	124, 432, 660, 796, 805, 994, 1543
LweI	GCATC	3 cut(s)	183, 987, 1081
MaeI	CTAG	3 cut(s)	696, 1028, 1699
MaeII	ACGT	2 cut(s)	1148, 1424
MaeIII	GTNAC	6 cut(s)	98, 886, 892, 949, 1220, 1291
MboII	GAAGA	7 cut(s)	19, 452, 855, 952, 1108, 1317, 1432
MfeI	CAATTG	4 cut(s)	369, 576, 588, 1011
MflI	RGATCY	2 cut(s)	188, 657
MhlI	GDGCHC	2 cut(s)	516, 1458
MlsI	TGGCCA	1 cut(s)	687
MluCI	AATT	8 cut(s)	252, 369, 451, 576, 588, 1011, 1172, 1243
MluNI	TGGCCA	1 cut(s)	687
Mly113I	GGCGCC	1 cut(s)	1062
MlyI	GAGTC	3 cut(s)	1101, 1217, 1457
MmeI	TCCRAC	4 cut(s)	339, 502, 1080, 1431
Mox20I	TGGCCA	1 cut(s)	687
Mph1103I	ATGCAT	1 cut(s)	176
MroXI	GAANNNNTTC	1 cut(s)	455
MscI	TGGCCA	1 cut(s)	687
MseI	TTAA	7 cut(s)	255, 306, 635, 753, 795, 1382, 1599
MslI	CAYNNNNRTG	1 cut(s)	857
Msp20I	TGGCCA	1 cut(s)	687
MspA1I	CMGCKG	1 cut(s)	713
MspCI	CTTAAG	1 cut(s)	634
MspI	CCGG	7 cut(s)	707, 937, 954, 1550, 1629, 1668, 1686
MspR9I	CCNGG	4 cut(s)	233, 937, 1492, 1551
MunI	CAATTG	4 cut(s)	369, 576, 588, 1011
Mva1269I	GAATGC	1 cut(s)	498
MvaI	CCWGG	2 cut(s)	233, 1492
MvnI	CGCG	5 cut(s)	197, 464, 655, 713, 1157
MwoI	GCNNNNNNNGC	9 cut(s)	137, 180, 424, 495, 520, 770, 815, 818, 1004
NarI	GGCGCC	1 cut(s)	1062
NciI	CCSGG	2 cut(s)	937, 1551
NcoI	CCATGG	2 cut(s)	945, 1415
NlaIII	CATG	9 cut(s)	174, 418, 529, 686, 922, 949, 1419, 1447, 1595
NlaIV	GGNNCC	5 cut(s)	190, 659, 934, 1063, 1548
NmuCI	GTSAC	4 cut(s)	892, 949, 1220, 1291
NruI	TCGCGA	1 cut(s)	197
NsiI	ATGCAT	1 cut(s)	176
NspI	RCATGY	2 cut(s)	174, 418
PaeI	GCATGC	1 cut(s)	174
PagI	TCATGA	1 cut(s)	525
PciSI	GCTCTTC	1 cut(s)	435
PctI	GAATGC	1 cut(s)	498
PdmI	GAANNNNTTC	1 cut(s)	455
PfeI	GAWTC	4 cut(s)	245, 362, 1393, 1540
PflFI	GACNNNGTC	1 cut(s)	1613
PleI	GAGTC	3 cut(s)	1100, 1217, 1456
PluTI	GGCGCC	1 cut(s)	1065
PpsI	GAGTC	3 cut(s)	1100, 1217, 1456
PshAI	GACNNNNGTC	1 cut(s)	947
Psp124BI	GAGCTC	2 cut(s)	516, 1458
Psp6I	CCWGG	2 cut(s)	231, 1490
PspEI	GGTNACC	1 cut(s)	949
PspFI	CCCAGC	1 cut(s)	866
PspGI	CCWGG	2 cut(s)	231, 1490
PspN4I	GGNNCC	5 cut(s)	190, 659, 934, 1063, 1548
PspPI	GGNCC	4 cut(s)	106, 932, 1547, 1631
PstI	CTGCAG	2 cut(s)	1127, 1352
PsuI	RGATCY	2 cut(s)	188, 657
PsyI	GACNNNGTC	1 cut(s)	1613
RruI	TCGCGA	1 cut(s)	197
RsaI	GTAC	2 cut(s)	1154, 1679
RsaNI	GTAC	2 cut(s)	1153, 1678
RseI	CAYNNNNRTG	1 cut(s)	857
SacI	GAGCTC	2 cut(s)	516, 1458
SacII	CCGCGG	1 cut(s)	714
SapI	GCTCTTC	1 cut(s)	435
SaqAI	TTAA	7 cut(s)	255, 306, 635, 753, 795, 1382, 1599
Sau96I	GGNCC	4 cut(s)	106, 932, 1547, 1631
SchI	GAGTC	3 cut(s)	1101, 1217, 1457
ScrFI	CCNGG	4 cut(s)	233, 937, 1492, 1551
SduI	GDGCHC	2 cut(s)	516, 1458
SfaNI	GCATC	3 cut(s)	183, 987, 1081
SfcI	CTRYAG	3 cut(s)	78, 1123, 1348
SfoI	GGCGCC	1 cut(s)	1063
Sfr303I	CCGCGG	1 cut(s)	714
SgrBI	CCGCGG	1 cut(s)	714
SinI	GGWCC	4 cut(s)	106, 932, 1547, 1631
SmiMI	CAYNNNNRTG	1 cut(s)	857
SmlI	CTYRAG	2 cut(s)	634, 662
SmoI	CTYRAG	2 cut(s)	634, 662
SphI	GCATGC	1 cut(s)	174
Sse9I	AATT	8 cut(s)	252, 369, 451, 576, 588, 1011, 1172, 1243
SspDI	GGCGCC	1 cut(s)	1061
SspMI	CTAG	3 cut(s)	696, 1028, 1699
SstI	GAGCTC	2 cut(s)	516, 1458
StyD4I	CCNGG	4 cut(s)	231, 935, 1490, 1549
StyI	CCWWGG	4 cut(s)	184, 570, 945, 1415
TaaI	ACNGT	8 cut(s)	262, 792, 892, 1187, 1204, 1291, 1438, 1677
TaiI	ACGT	2 cut(s)	1151, 1427
TaqI	TCGA	4 cut(s)	177, 320, 1180, 1512
TasI	AATT	8 cut(s)	252, 369, 451, 576, 588, 1011, 1172, 1243
TauI	GCSGC	3 cut(s)	239, 713, 724
TfiI	GAWTC	4 cut(s)	245, 362, 1393, 1540
Tru1I	TTAA	7 cut(s)	255, 306, 635, 753, 795, 1382, 1599
Tru9I	TTAA	7 cut(s)	255, 306, 635, 753, 795, 1382, 1599
TscAI	CASTG	2 cut(s)	1092, 1296
TseFI	GTSAC	4 cut(s)	892, 949, 1220, 1291
TseI	GCWGC	7 cut(s)	137, 445, 648, 809, 818, 1007, 1556
Tsp45I	GTSAC	4 cut(s)	892, 949, 1220, 1291
TspDTI	ATGAA	4 cut(s)	542, 907, 1201, 1456
TspGWI	ACGGA	5 cut(s)	170, 181, 945, 1035, 1386
TspRI	CASTG	2 cut(s)	1092, 1296
Tth111I	GACNNNGTC	1 cut(s)	1613
Vha464I	CTTAAG	1 cut(s)	634
VpaK11BI	GGWCC	4 cut(s)	106, 932, 1547, 1631
XagI	CCTNNNNNAGG	1 cut(s)	230
XapI	RAATTY	1 cut(s)	1243
XceI	RCATGY	2 cut(s)	174, 418
XcmI	CCANNNNNNNNNTGG	1 cut(s)	568
XmnI	GAANNNNTTC	1 cut(s)	455
XspI	CTAG	3 cut(s)	696, 1028, 1699
Zsp2I	ATGCAT	1 cut(s)	176

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.