Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003768

Physical Location & Seq

Genomic Coordinates Forward (+)

49594128 .. 49596515

Gene Structure 2388 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G023810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1842 bp

ATGGAAAAGAAGGGAAAGGCAATAGTAGGTGGAATTTCAATCATCCTTGTGGTTGGAGCAGTAGTTGGTTGTGTAGCTGTTGTTTCACATAATCGCAGCAAAGGCAAGGATAGGCTTTCGACGAGTTCCAAGGCCGTCAATGCAATGTGTGAACCAACTGATTACAAGGAAGAATGCATTGACAGCCTGGCTGACGCTGCCAAGAATCAAAGCGCCACCCCAAAAGAACTTATCCGAGCTGCCATTGATTACACAATCGATAAGGTGAAAGCGGCAATAGAGAAATCAGATAGCATTATCAAAGAAGCTAAAAATGTCAGTTCCACTGGGAAAATGGCCTTCAAGGATTGCAATGACTTGTTGGAATCTTCAATCGAAAGTCTTCAATATTCGCTTTTGACACTCGTTGATACTGAAATGCAAAACATGAATAATAAAGAGGCTGATCTTAAGAACTGGTTCAGTGCTGCGATATCATTTCAGGAATCATGCATTGATGGGATTCCATACGAAGACCTTAAGAACCAAATGAACGAGGCAATGCTTCATGCTTCACAACTGACCAGCAACGCCTTAGCAATTGTGTCTTCCCTTGCTCCCATCCTAGGCAAATTCAATATCTCCATGAAAACTGACAGTAAAAGTTCAACCAGTCGTCGTCTCCTGGATGTAGAAATGGAAACGGATGAACATGGCACTTTTCCAGCATGGTTCCCAGCTGCAGATAGGAAGCTATTCGCACACCACAACGAGAACAATAACAATAGCAGCAACAACAACAACAACGACGACGTCGACAACAACGACAACAACAACGGGAGAGTCGACAACGACGACAACAACAACAACGGGAGAGTCGACAACGACGACAACAACAACAACGGGAGAGTTACACCCAACGCAGTAGTGGCAAAAGATGGGAGTGGACAATACAAAACAATTGGTGAAGCTCTTGCTGCGTACCCAAAGGGATTCCAGGGAAGATATGTTATATATGTGAAAGCTGGGATTTACAATGAGTATGTTACTGTGGCCAAGAATCAGGTCAATGTGTACATGTATGGAGATGGACCCCGGAGGACCATGATCACAGGCAGTAAAAGCTTTGCTGATGGCATCACCACCCAGGACACTGCCACATTCATCGTAATGGGGAATGGGTTCATTGGTAGAGCAATGGGATTTCAGAACACAGCCGGCCCCGAAAAGCATCAGGCAGTGGCACTCCGAGTCCAGTCGGAAATGTCAGCCTTCTTCAACTGCAGAATGGACGCCTACCAAGACACATTGTACGTGCAAGCATATCGCCAATTTTATCGCAACTGTGTCATATCCGGTACCGTTGACTTCATTTTTGGTGACTCATCAACAGTCATCCAGAATAGTTTACTCATTGTGAGGAAGCCTATGGACGGCCAAAAGAACATTGTGACTGCTCATGGAAGAAAAGATAAGCGGGAAACCACTGGTCTGGTTATCCAGAACTGCAGGATAGTCCCAGAACAGGCTTTGTTCCCAATGAGATTCCAGATTCAGTCATACTTGGGGCGGCCGTGGAAGATGTACTCGAGAACAGTTATCATGGAGTCCGAATTTGGGGACTTTATCCAACCAGCCGGTTGGCTAGAATGGGAAGGGACTTTTGGACTAGACACACTGTATTATGCAGAGTATGCAAATCGGGGTCCGGGTGCTGTAACCACAGGAAGGGTAAAATGGAACGGATACCATGTGATTACGGACAGAAATGAGGCTCTTCAATTCACTCCGGGTAATTTTCTAACAGGAGACCAATGGTTGAAAGACGCGGGTATACCTTACTTCCTGGGTTTAAAAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

613

Amino Acids

67.95

Weight (kDa)

5.77

Isoelectric Point (pI)

26.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	45 - 194	2.2e-29	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	300 - 597	1.2e-135	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003768)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47030 AT2G47040 AT2G47050 AT3G62170 AT3G62180 AT3G62180
fragaria_vesca	FvH4_1g26360 FvH4_4g21500
malus_domestica	MD16G1136400.v1.1 MD17G1283800.v1.1
prunus_persica	Prupe.1G105400_v2.0.a1 Prupe.3G003700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0486131 RchiOBHm_Chr4g0427511
rosa_laevigata	RLG00000007232 RLG00000023101
rosa_multiflora	Rmu_sc0001006.1_g000002 Rmu_sc0009627.1_g000001 Rmu_sc0017895.1_g000001 Rmu_sc0017895.1_g000002
rosa_roxburghii	Rroxscaffold_5G00369150 Rroxscaffold_6G00396030
rosa_rugosa	Rorug03G0223700 Rorug04G0218200
rosa_samantha	Rh3AG273500 Rh3BG308600 Rh3CG307100 Rh3DG303400 Rh4AG274700 Rh4BG280500 Rh4CG295800 Rh4DG277900
rosa_wichuraiana	Rw3G024240 Rw4G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	795
Acc65I	GGTACC	1 cut(s)	1337
AccB1I	GGYRCC	1 cut(s)	1337
AccI	GTMKAC	4 cut(s)	795, 825, 858, 1813
AccII	CGCG	1 cut(s)	1808
AciI	CCGC	4 cut(s)	272, 1456, 1549, 1808
AcoI	YGGCCR	3 cut(s)	1032, 1414, 1550
AcsI	RAATTY	3 cut(s)	33, 611, 1592
AcyI	GRCGYC	2 cut(s)	792, 1272
AfaI	GTAC	5 cut(s)	962, 1055, 1292, 1339, 1565
AfiI	CCNNNNNNNGG	5 cut(s)	605, 1412, 1504, 1596, 1707
AflII	CTTAAG	2 cut(s)	449, 518
AflIII	ACRYGT	1 cut(s)	1056
AgsI	TTSAA	9 cut(s)	39, 343, 372, 386, 616, 648, 1258, 1760, 1801
AjnI	CCWGG	5 cut(s)	186, 663, 975, 1125, 1824
AjuI	GAANNNNNNNTTGG	2 cut(s)	1626, 1658
AluBI	AGCT	8 cut(s)	77, 239, 308, 719, 733, 950, 1004, 1104
AluI	AGCT	8 cut(s)	77, 239, 308, 719, 733, 950, 1004, 1104
Alw26I	GTCTC	2 cut(s)	665, 1782
Ama87I	CYCGRG	1 cut(s)	1567
AoxI	GGCC	6 cut(s)	132, 336, 1032, 1198, 1414, 1550
ApeKI	GCWGC	7 cut(s)	96, 197, 239, 467, 719, 768, 956
ApoI	RAATTY	3 cut(s)	33, 611, 1592
ArsI	GACNNNNNNTTYG	2 cut(s)	1337, 1369
Asp700I	GAANNNNTTC	4 cut(s)	381, 458, 734, 1160
Asp718I	GGTACC	1 cut(s)	1337
AspA2I	CCTAGG	1 cut(s)	604
AspLEI	GCGC	1 cut(s)	215
AspS9I	GGNCC	4 cut(s)	1070, 1080, 1199, 1685
AsuC2I	CCSGG	3 cut(s)	1075, 1689, 1770
AsuHPI	GGTGA	4 cut(s)	277, 956, 1111, 1370
AvaI	CYCGRG	1 cut(s)	1567
AvaII	GGWCC	3 cut(s)	1070, 1080, 1685
AvrII	CCTAGG	1 cut(s)	604
BalI	TGGCCA	1 cut(s)	1034
BanI	GGYRCC	1 cut(s)	1337
BbsI	GAAGAC	3 cut(s)	374, 519, 579
BbvI	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 943
BccI	CCATC	5 cut(s)	491, 608, 911, 1061, 1106
BceAI	ACGGC	3 cut(s)	119, 1429, 1537
BcgI	CGANNNNNNTGC	2 cut(s)	1286, 1320
BciT130I	CCWGG	5 cut(s)	188, 665, 977, 1127, 1826
BciVI	GTATCC	1 cut(s)	1718
BclI	TGATCA	1 cut(s)	1086
BcnI	CCSGG	3 cut(s)	1075, 1689, 1770
BcoDI	GTCTC	2 cut(s)	665, 1782
BfaI	CTAG	4 cut(s)	605, 1625, 1649, 1840
BfmI	CTRYAG	3 cut(s)	720, 1261, 1486
BfoI	RGCGCY	1 cut(s)	216
BfrI	CTTAAG	2 cut(s)	449, 518
BfuI	GTATCC	1 cut(s)	1718
BisI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 957, 1550
BlnI	CCTAGG	1 cut(s)	604
BlsI	GCNGC	9 cut(s)	98, 199, 241, 274, 469, 721, 770, 958, 1551
Bme1390I	CCNGG	8 cut(s)	188, 665, 977, 1075, 1127, 1689, 1770, 1826
Bme18I	GGWCC	3 cut(s)	1070, 1080, 1685
BmeT110I	CYCGRG	1 cut(s)	1567
BmgT120I	GGNCC	4 cut(s)	1070, 1080, 1199, 1685
BmiI	GGNNCC	5 cut(s)	713, 1072, 1201, 1339, 1686
BmrFI	CCNGG	8 cut(s)	188, 665, 977, 1075, 1127, 1689, 1770, 1826
BmrI	ACTGGG	1 cut(s)	336
BmsI	GCATC	2 cut(s)	1125, 1219
BmuI	ACTGGG	1 cut(s)	336
BpiI	GAAGAC	3 cut(s)	374, 519, 579
Bpu10I	CCTNAGC	1 cut(s)	574
BpuMI	CCSGG	3 cut(s)	1075, 1689, 1770
Bsa29I	ATCGAT	1 cut(s)	258
BsaAI	YACGTR	1 cut(s)	1294
BsaHI	GRCGYC	2 cut(s)	792, 1272
BsaI	GGTCTC	1 cut(s)	1782
BsaJI	CCNNGG	7 cut(s)	129, 604, 976, 1073, 1125, 1553, 1825
BsaWI	WCCGGW	1 cut(s)	1334
BsaXI	ACNNNNNCTCC	2 cut(s)	48, 78
Bsc4I	CCNNNNNNNGG	5 cut(s)	605, 1412, 1504, 1596, 1707
Bse118I	RCCGGY	2 cut(s)	1196, 1616
Bse1I	ACTGG	5 cut(s)	331, 461, 651, 1234, 1471
Bse3DI	GCAATG	4 cut(s)	150, 358, 546, 1182
BseBI	CCWGG	5 cut(s)	188, 665, 977, 1127, 1826
BseCI	ATCGAT	1 cut(s)	258
BseDI	CCNNGG	7 cut(s)	129, 604, 976, 1073, 1125, 1553, 1825
BseGI	GGATG	5 cut(s)	42, 600, 673, 691, 1374
BseLI	CCNNNNNNNGG	5 cut(s)	605, 1412, 1504, 1596, 1707
BseMI	GCAATG	4 cut(s)	150, 358, 546, 1182
BseNI	ACTGG	5 cut(s)	331, 461, 651, 1234, 1471
BseX3I	CGGCCG	1 cut(s)	1550
BseXI	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 943
BseYI	CCCAGC	2 cut(s)	715, 1004
Bsh1236I	CGCG	1 cut(s)	1808
Bsh1285I	CGRYCG	1 cut(s)	1553
BshFI	GGCC	6 cut(s)	134, 338, 1034, 1200, 1416, 1552
BshNI	GGYRCC	1 cut(s)	1337
BshVI	ATCGAT	1 cut(s)	258
BsiEI	CGRYCG	1 cut(s)	1553
BsiHKCI	CYCGRG	1 cut(s)	1567
BsiSI	CCGG	6 cut(s)	1075, 1197, 1335, 1617, 1688, 1769
BslFI	GGGAC	3 cut(s)	1481, 1613, 1651
BslI	CCNNNNNNNGG	5 cut(s)	605, 1412, 1504, 1596, 1707
BsmAI	GTCTC	2 cut(s)	665, 1782
BsmBI	CGTCTC	1 cut(s)	665
BsmFI	GGGAC	3 cut(s)	1481, 1613, 1651
BsmI	GAATGC	1 cut(s)	179
BsnI	GGCC	6 cut(s)	134, 338, 1034, 1200, 1416, 1552
Bso31I	GGTCTC	1 cut(s)	1782
BsoBI	CYCGRG	1 cut(s)	1567
Bsp1407I	TGTACA	1 cut(s)	1053
Bsp143I	GATC	2 cut(s)	445, 1086
BspACI	CCGC	4 cut(s)	272, 1456, 1549, 1808
BspANI	GGCC	6 cut(s)	134, 338, 1034, 1200, 1416, 1552
BspDI	ATCGAT	1 cut(s)	258
BspFNI	CGCG	1 cut(s)	1808
BspLI	GGNNCC	5 cut(s)	713, 1072, 1201, 1339, 1686
BspMAI	CTGCAG	3 cut(s)	724, 1265, 1490
BspQI	GCTCTTC	1 cut(s)	1761
BspT107I	GGYRCC	1 cut(s)	1337
BspTI	CTTAAG	2 cut(s)	449, 518
BspTNI	GGTCTC	1 cut(s)	1782
BsrDI	GCAATG	4 cut(s)	150, 358, 546, 1182
BsrFI	RCCGGY	2 cut(s)	1196, 1616
BsrGI	TGTACA	1 cut(s)	1053
BsrI	ACTGG	5 cut(s)	331, 461, 651, 1234, 1471
BssAI	RCCGGY	2 cut(s)	1196, 1616
BssECI	CCNNGG	7 cut(s)	129, 604, 976, 1073, 1125, 1553, 1825
BssMI	GATC	2 cut(s)	445, 1086
BssNAI	GTATAC	1 cut(s)	1814
BssNI	GRCGYC	2 cut(s)	792, 1272
BssT1I	CCWWGG	2 cut(s)	129, 604
Bst1107I	GTATAC	1 cut(s)	1814
Bst2UI	CCWGG	5 cut(s)	188, 665, 977, 1127, 1826
Bst4CI	ACNGT	7 cut(s)	638, 1030, 1325, 1342, 1372, 1576, 1659
Bst6I	CTCTTC	1 cut(s)	1761
BstACI	GRCGYC	2 cut(s)	792, 1272
BstAFI	CTTAAG	2 cut(s)	449, 518
BstAPI	GCANNNNNTGC	1 cut(s)	1673
BstAUI	TGTACA	1 cut(s)	1053
BstBAI	YACGTR	1 cut(s)	1294
BstC8I	GCNNGC	2 cut(s)	1198, 1299
BstDEI	CTNAG	1 cut(s)	574
BstDSI	CCRYGG	1 cut(s)	1553
BstF5I	GGATG	5 cut(s)	42, 600, 673, 691, 1374
BstFNI	CGCG	1 cut(s)	1808
BstH2I	RGCGCY	1 cut(s)	216
BstHHI	GCGC	1 cut(s)	215
BstKTI	GATC	2 cut(s)	448, 1089
BstMAI	GTCTC	2 cut(s)	665, 1782
BstMBI	GATC	2 cut(s)	445, 1086
BstMCI	CGRYCG	1 cut(s)	1553
BstMWI	GCNNNNNNNGC	8 cut(s)	102, 140, 183, 197, 908, 956, 1101, 1673
BstNI	CCWGG	5 cut(s)	188, 665, 977, 1127, 1826
BstNSI	RCATGY	1 cut(s)	1060
BstSCI	CCNGG	8 cut(s)	186, 663, 975, 1073, 1125, 1687, 1768, 1824
BstSFI	CTRYAG	3 cut(s)	720, 1261, 1486
BstUI	CGCG	1 cut(s)	1808
BstV1I	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 943
BstV2I	GAAGAC	3 cut(s)	374, 519, 579
BstXI	CCANNNNNNTGG	2 cut(s)	1471, 1620
BstZ17I	GTATAC	1 cut(s)	1814
BstZI	CGGCCG	1 cut(s)	1550
Bsu15I	ATCGAT	1 cut(s)	258
BsuI	GTATCC	1 cut(s)	1718
BsuRI	GGCC	6 cut(s)	134, 338, 1034, 1200, 1416, 1552
BsuTUI	ATCGAT	1 cut(s)	258
BtgI	CCRYGG	1 cut(s)	1553
BtsCI	GGATG	5 cut(s)	42, 600, 673, 691, 1374
BtsI	GCAGTG	2 cut(s)	1131, 1224
BtsIMutI	CAGTG	6 cut(s)	324, 469, 1131, 1224, 1464, 1655
Cac8I	GCNNGC	2 cut(s)	1198, 1299
CfoI	GCGC	1 cut(s)	215
Cfr10I	RCCGGY	2 cut(s)	1196, 1616
Cfr13I	GGNCC	4 cut(s)	1070, 1080, 1199, 1685
ClaI	ATCGAT	1 cut(s)	258
CseI	GACGC	3 cut(s)	203, 1280, 1814
Csp6I	GTAC	5 cut(s)	961, 1054, 1291, 1338, 1564
CviQI	GTAC	5 cut(s)	961, 1054, 1291, 1338, 1564
DdeI	CTNAG	1 cut(s)	574
DpnI	GATC	2 cut(s)	447, 1088
DpnII	GATC	2 cut(s)	445, 1086
DraI	TTTAAA	1 cut(s)	1833
EaeI	YGGCCR	3 cut(s)	1032, 1414, 1550
EagI	CGGCCG	1 cut(s)	1550
Eam1104I	CTCTTC	1 cut(s)	1761
EarI	CTCTTC	1 cut(s)	1761
EclXI	CGGCCG	1 cut(s)	1550
Eco130I	CCWWGG	2 cut(s)	129, 604
Eco31I	GGTCTC	1 cut(s)	1782
Eco32I	GATATC	1 cut(s)	474
Eco47I	GGWCC	3 cut(s)	1070, 1080, 1685
Eco52I	CGGCCG	1 cut(s)	1550
Eco88I	CYCGRG	1 cut(s)	1567
EcoRII	CCWGG	5 cut(s)	186, 663, 975, 1125, 1824
EcoRV	GATATC	1 cut(s)	474
EcoT14I	CCWWGG	2 cut(s)	129, 604
EcoT22I	ATGCAT	2 cut(s)	179, 494
ErhI	CCWWGG	2 cut(s)	129, 604
Esp3I	CGTCTC	1 cut(s)	665
FaqI	GGGAC	3 cut(s)	1481, 1613, 1651
FauI	CCCGC	2 cut(s)	1449, 1801
FbaI	TGATCA	1 cut(s)	1086
FblI	GTMKAC	4 cut(s)	795, 825, 858, 1813
Fnu4HI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 957, 1550
FokI	GGATG	5 cut(s)	29, 587, 680, 698, 1361
Fsp4HI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 957, 1550
FspBI	CTAG	4 cut(s)	605, 1625, 1649, 1840
GlaI	GCGC	1 cut(s)	214
GluI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 957, 1550
GsaI	CCCAGC	2 cut(s)	719, 1008
HaeII	RGCGCY	1 cut(s)	216
HaeIII	GGCC	6 cut(s)	134, 338, 1034, 1200, 1416, 1552
HapII	CCGG	6 cut(s)	1075, 1197, 1335, 1617, 1688, 1769
HgaI	GACGC	3 cut(s)	203, 1280, 1814
HhaI	GCGC	1 cut(s)	215
Hin1I	GRCGYC	2 cut(s)	792, 1272
Hin6I	GCGC	1 cut(s)	213
HinP1I	GCGC	1 cut(s)	213
HincII	GTYRAC	4 cut(s)	796, 826, 859, 1345
HindII	GTYRAC	4 cut(s)	796, 826, 859, 1345
HindIII	AAGCTT	1 cut(s)	1102
HpaII	CCGG	6 cut(s)	1075, 1197, 1335, 1617, 1688, 1769
HphI	GGTGA	4 cut(s)	277, 956, 1111, 1370
Hpy166II	GTNNAC	9 cut(s)	152, 796, 826, 859, 926, 1054, 1345, 1388, 1814
Hpy188I	TCNGA	6 cut(s)	236, 289, 1188, 1229, 1240, 1591
Hpy188III	TCNNGA	5 cut(s)	482, 1378, 1480, 1528, 1569
Hpy8I	GTNNAC	9 cut(s)	152, 796, 826, 859, 926, 1054, 1345, 1388, 1814
Hpy99I	CGWCG	7 cut(s)	124, 660, 791, 794, 797, 836, 869
HpyAV	CCTTC	5 cut(s)	4, 349, 1261, 1628, 1701
HpyCH4III	ACNGT	7 cut(s)	638, 1030, 1325, 1342, 1372, 1576, 1659
HpyCH4IV	ACGT	2 cut(s)	792, 1293
HpyF10VI	GCNNNNNNNGC	8 cut(s)	102, 140, 183, 197, 908, 956, 1101, 1673
HpyF3I	CTNAG	1 cut(s)	574
HpySE526I	ACGT	2 cut(s)	792, 1293
Hsp92I	GRCGYC	2 cut(s)	792, 1272
HspAI	GCGC	1 cut(s)	213
KpnI	GGTACC	1 cut(s)	1341
KroI	GCCGGC	1 cut(s)	1196
KroNI	GCCGGC	1 cut(s)	1198
Ksp22I	TGATCA	1 cut(s)	1086
Kzo9I	GATC	2 cut(s)	445, 1086
LguI	GCTCTTC	1 cut(s)	1761
LmnI	GCTCC	2 cut(s)	56, 601
Lsp1109I	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 943
LweI	GCATC	2 cut(s)	1125, 1219
MaeI	CTAG	4 cut(s)	605, 1625, 1649, 1840
MaeII	ACGT	2 cut(s)	792, 1293
MaeIII	GTNAC	5 cut(s)	889, 1024, 1358, 1429, 1696
MalI	GATC	2 cut(s)	447, 1088
MboI	GATC	2 cut(s)	445, 1086
MfeI	CAATTG	2 cut(s)	579, 939
MlsI	TGGCCA	1 cut(s)	1034
MluCI	AATT	8 cut(s)	33, 579, 611, 939, 1310, 1592, 1760, 1774
MluNI	TGGCCA	1 cut(s)	1034
MlyI	GAGTC	5 cut(s)	831, 864, 1239, 1355, 1595
MmeI	TCCRAC	4 cut(s)	34, 342, 1218, 1633
MnlI	CCTC	5 cut(s)	433, 529, 1071, 1392, 1744
Mox20I	TGGCCA	1 cut(s)	1034
Mph1103I	ATGCAT	2 cut(s)	179, 494
MroNI	GCCGGC	1 cut(s)	1196
MroXI	GAANNNNTTC	4 cut(s)	381, 458, 734, 1160
MscI	TGGCCA	1 cut(s)	1034
MseI	TTAA	3 cut(s)	450, 519, 1832
MslI	CAYNNNNRTG	2 cut(s)	47, 1148
Msp20I	TGGCCA	1 cut(s)	1034
MspA1I	CMGCKG	1 cut(s)	719
MspCI	CTTAAG	2 cut(s)	449, 518
MspI	CCGG	6 cut(s)	1075, 1197, 1335, 1617, 1688, 1769
MspR9I	CCNGG	8 cut(s)	188, 665, 977, 1075, 1127, 1689, 1770, 1826
MunI	CAATTG	2 cut(s)	579, 939
Mva1269I	GAATGC	1 cut(s)	179
MvaI	CCWGG	5 cut(s)	188, 665, 977, 1127, 1826
MvnI	CGCG	1 cut(s)	1808
MwoI	GCNNNNNNNGC	8 cut(s)	102, 140, 183, 197, 908, 956, 1101, 1673
NaeI	GCCGGC	1 cut(s)	1198
NciI	CCSGG	3 cut(s)	1075, 1689, 1770
NdeII	GATC	2 cut(s)	445, 1086
NgoMIV	GCCGGC	1 cut(s)	1196
NlaIV	GGNNCC	5 cut(s)	713, 1072, 1201, 1339, 1686
NmuCI	GTSAC	2 cut(s)	1358, 1429
NsiI	ATGCAT	2 cut(s)	179, 494
NspI	RCATGY	1 cut(s)	1060
PaeR7I	CTCGAG	1 cut(s)	1567
PciI	ACATGT	1 cut(s)	1056
PciSI	GCTCTTC	1 cut(s)	1761
PcsI	WCGNNNNNNNCGW	6 cut(s)	792, 801, 822, 831, 855, 864
PctI	GAATGC	1 cut(s)	179
PdiI	GCCGGC	1 cut(s)	1198
PdmI	GAANNNNTTC	4 cut(s)	381, 458, 734, 1160
PfeI	GAWTC	8 cut(s)	205, 365, 485, 502, 972, 1039, 1524, 1531
PflFI	GACNNNGTC	1 cut(s)	791
PfoI	TCCNGGA	1 cut(s)	663
PkrI	GCNGC	9 cut(s)	98, 199, 241, 274, 469, 721, 770, 958, 1551
PleI	GAGTC	5 cut(s)	830, 863, 1238, 1355, 1594
PpsI	GAGTC	5 cut(s)	830, 863, 1238, 1355, 1594
Ppu21I	YACGTR	1 cut(s)	1294
PscI	ACATGT	1 cut(s)	1056
Psp6I	CCWGG	5 cut(s)	186, 663, 975, 1125, 1824
PspFI	CCCAGC	2 cut(s)	715, 1004
PspGI	CCWGG	5 cut(s)	186, 663, 975, 1125, 1824
PspN4I	GGNNCC	5 cut(s)	713, 1072, 1201, 1339, 1686
PspPI	GGNCC	4 cut(s)	1070, 1080, 1199, 1685
PstI	CTGCAG	3 cut(s)	724, 1265, 1490
PsyI	GACNNNGTC	1 cut(s)	791
PvuII	CAGCTG	1 cut(s)	719
RsaI	GTAC	5 cut(s)	962, 1055, 1292, 1339, 1565
RsaNI	GTAC	5 cut(s)	961, 1054, 1291, 1338, 1564
RseI	CAYNNNNRTG	2 cut(s)	47, 1148
SalI	GTCGAC	3 cut(s)	794, 824, 857
SapI	GCTCTTC	1 cut(s)	1761
SaqAI	TTAA	3 cut(s)	450, 519, 1832
SatI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 957, 1550
Sau3AI	GATC	2 cut(s)	445, 1086
Sau96I	GGNCC	4 cut(s)	1070, 1080, 1199, 1685
SchI	GAGTC	5 cut(s)	831, 864, 1239, 1355, 1595
ScrFI	CCNGG	8 cut(s)	188, 665, 977, 1075, 1127, 1689, 1770, 1826
SfaNI	GCATC	2 cut(s)	1125, 1219
SfcI	CTRYAG	3 cut(s)	720, 1261, 1486
Sfr274I	CTCGAG	1 cut(s)	1567
SinI	GGWCC	3 cut(s)	1070, 1080, 1685
SlaI	CTCGAG	1 cut(s)	1567
SmiMI	CAYNNNNRTG	2 cut(s)	47, 1148
SmlI	CTYRAG	3 cut(s)	449, 518, 1567
SmoI	CTYRAG	3 cut(s)	449, 518, 1567
Sse9I	AATT	8 cut(s)	33, 579, 611, 939, 1310, 1592, 1760, 1774
SsiI	CCGC	4 cut(s)	272, 1456, 1549, 1808
SspI	AATATT	1 cut(s)	389
SspMI	CTAG	4 cut(s)	605, 1625, 1649, 1840
StyD4I	CCNGG	8 cut(s)	186, 663, 975, 1073, 1125, 1687, 1768, 1824
StyI	CCWWGG	2 cut(s)	129, 604
TaaI	ACNGT	7 cut(s)	638, 1030, 1325, 1342, 1372, 1576, 1659
TaiI	ACGT	2 cut(s)	795, 1296
TaqI	TCGA	7 cut(s)	119, 258, 375, 795, 825, 858, 1568
TasI	AATT	8 cut(s)	33, 579, 611, 939, 1310, 1592, 1760, 1774
TatI	WGTACW	2 cut(s)	1053, 1563
TauI	GCSGC	2 cut(s)	275, 1552
TfiI	GAWTC	8 cut(s)	205, 365, 485, 502, 972, 1039, 1524, 1531
Tru1I	TTAA	3 cut(s)	450, 519, 1832
Tru9I	TTAA	3 cut(s)	450, 519, 1832
TscAI	CASTG	6 cut(s)	331, 469, 1138, 1224, 1471, 1662
TseFI	GTSAC	2 cut(s)	1358, 1429
TseI	GCWGC	7 cut(s)	96, 197, 239, 467, 719, 768, 956
Tsp45I	GTSAC	2 cut(s)	1358, 1429
TspDTI	ATGAA	8 cut(s)	443, 536, 545, 641, 702, 1132, 1153, 1339
TspGWI	ACGGA	3 cut(s)	698, 1737, 1754
TspRI	CASTG	6 cut(s)	331, 469, 1138, 1224, 1471, 1662
Tth111I	GACNNNGTC	1 cut(s)	791
Vha464I	CTTAAG	2 cut(s)	449, 518
VpaK11BI	GGWCC	3 cut(s)	1070, 1080, 1685
XapI	RAATTY	3 cut(s)	33, 611, 1592
XceI	RCATGY	1 cut(s)	1060
XcmI	CCANNNNNNNNNTGG	2 cut(s)	331, 904
XhoI	CTCGAG	1 cut(s)	1567
XmaJI	CCTAGG	1 cut(s)	604
XmiI	GTMKAC	4 cut(s)	795, 825, 858, 1813
XmnI	GAANNNNTTC	4 cut(s)	381, 458, 734, 1160
XspI	CTAG	4 cut(s)	605, 1625, 1649, 1840
ZraI	GACGTC	1 cut(s)	793
Zsp2I	ATGCAT	2 cut(s)	179, 494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.