Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003768

Physical Location & Seq

Genomic Coordinates Forward (+)

52757008 .. 52759317

Gene Structure 2310 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ39643

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1857 bp

ATGGAAAAGAAGGGAAAGGCAATAGTAGGTGGAATTTCAATCATCCTTGTGGTTGGAGCAGTAGTTGGTTGTGTAGCTGTTGTTTCACATAATCGCAGCAAAGGCAAGGATAGGCTTTCGACGAGTTCCAAGGCCGTCAATGCAATGTGTGAACCAACTGATTACAAGGAAGAATGCATTGACAGCCTGGCTGACGCTGCCAAGAATCAAAGCGCCACCCCAAAAGAACTTATCCGAGCTGCCATTGATTACACAATCGATAAGGTGAAAGCGGCAATAGAGAAATCAGATAGCATTATCAAAGAAGCTAAAAATGTCAGTTCCACTGGGAAAATGGCCTTCAAGGATTGCAATGACTTGTTGGAATCTTCAATCGAAAGTCTTCAATATTCGCTTTTGACACTCGTTGATACTGAAATGCAAAACATGAATAATAAAGAGGCTGATCTTAAGAACTGGTTCAGTGCTGCGATATCATTTCAGGAATCATGCATTGATGGGATTCCATACGAAGACCTTAAGAACCAAATGAACGAGGCAATGCTCCATGCTTCACAACTGACCAGCAACGCCTTAGCAATTGTGTCTTCCCTTGCTCCCATCCTAGGCAAATTCAATATCTCCATGAAAACTGACAGTAAAAGTTCAACCAGTCGTCGTCTCCTGGATGTAGAAATGGAAACGGATGAACATGGCACTTTTCCAGCATGGTTCCCAGCTGCAGATAGGAAGCTATTTGCACACCACAACGAGAACAATAACAATAGCAGCAACAACGACGTCGTCGACAACGACGACGACAACAACAACAACAACAACAACAACAACGGGAGAGTCGACAACGACGACAACAACAACAACGGGAGAGTCGACAACGTCGACAACAACAACAACAACGGGAGAGTTACACCCAACGCAGTAGTGGCAAAAGATGGGAGTGGACAATACAAAACAATTGGTGAAGCTCTTGCTGCGTACCCAAAGGGATTCCAGGGAAGATATGTTATATATGTGAAAGCTGGGATTTACAATGAGTATGTTACTGTGGCCAAGAATCAGGTCAATGTGTACATGTATGGAGATGGACCCCGGAGGACCATGATCACAGGCAGTAAAAGCTTTGCTGATGGCATCACCACCCAGGACACTGCCACATTCATCGTAATGGGGAATGGGTTCATTGGTAGAGCAATGGGATTTCAGAACACAGCCGGCCCCGAAAAGCATCAGGCAGTGGCACTCCGAGTCCAGTCGGAAATGTCAGCCTTCTTCAACTGCAGAATGGACGCCTACCAAGACACATTGTACGTGCAAGCATATCGCCAATTTTATCGCAACTGTGTCATATCCGGTACCGTTGACTTCATTTTTGGTGACTCATCAACAGTCATCCAGAATAGTTTACTCATTGTGAGGAAGCCTATGGACGGCCAAAAGAACATTGTGACTGCTCATGGAAGAAAAGATAAGCGGGAAACCACCGGTCTGGTTATCCAGAACTGCAGGATAGTCCCAGAACAGGCTTTGTTCCCAATGAGATTCCAGATTCAGTCATACTTGGGGCGGCCGTGGAAGATGTACTCGAGAACAGTTATCATGGAGTCCGAATTTGGGGACTTTATCCAACCAGCCGGTTGGCTAGAATGGGAAGGGACTTTTGGACTAGACACACTGTATTATGCAGAGTATGCAAATCGGGGTCCGGGTGCTGTAACCACAGAAAGGGTAAAATGGAAAGGATACCATGTGATTACGGACAGAAATGAGGCTCTTCAATTCACTCCGGGTAATTTTCTAACAGGAGACCAATGGTTGAAAGACGCGGGTATACCTTACTTCCTGGGTTTAAAAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

618

Amino Acids

68.57

Weight (kDa)

5.78

Isoelectric Point (pI)

26.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	45 - 194	2.2e-29	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	305 - 602	5.4e-136	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003768)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47030 AT2G47040 AT2G47050 AT3G62170 AT3G62180 AT3G62180
fragaria_vesca	FvH4_1g26360 FvH4_4g21500
malus_domestica	MD16G1136400.v1.1 MD17G1283800.v1.1
prunus_persica	Prupe.1G105400_v2.0.a1 Prupe.3G003700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0486131 RchiOBHm_Chr4g0427511
rosa_laevigata	RLG00000007232 RLG00000023101
rosa_multiflora	Rmu_sc0001006.1_g000002 Rmu_sc0009627.1_g000001 Rmu_sc0017895.1_g000001 Rmu_sc0017895.1_g000002
rosa_roxburghii	Rroxscaffold_5G00369150 Rroxscaffold_6G00396030
rosa_rugosa	Rorug03G0223700 Rorug04G0218200
rosa_samantha	Rh3AG273500 Rh3BG308600 Rh3CG307100 Rh3DG303400 Rh4AG274700 Rh4BG280500 Rh4CG295800 Rh4DG277900
rosa_wichuraiana	Rw3G024240 Rw4G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	783
Acc65I	GGTACC	1 cut(s)	1352
AccB1I	GGYRCC	1 cut(s)	1352
AccI	GTMKAC	5 cut(s)	786, 837, 870, 879, 1828
AccII	CGCG	1 cut(s)	1823
AciI	CCGC	4 cut(s)	272, 1471, 1564, 1823
AcoI	YGGCCR	3 cut(s)	1047, 1429, 1565
AcsI	RAATTY	3 cut(s)	33, 611, 1607
AcyI	GRCGYC	2 cut(s)	780, 1287
AfaI	GTAC	5 cut(s)	977, 1070, 1307, 1354, 1580
AfiI	CCNNNNNNNGG	5 cut(s)	605, 1427, 1519, 1611, 1722
AflII	CTTAAG	2 cut(s)	449, 518
AflIII	ACRYGT	1 cut(s)	1071
AgeI	ACCGGT	1 cut(s)	1481
AgsI	TTSAA	9 cut(s)	39, 343, 372, 386, 616, 648, 1273, 1775, 1816
AjnI	CCWGG	5 cut(s)	186, 663, 990, 1140, 1839
AjuI	GAANNNNNNNTTGG	2 cut(s)	1641, 1673
AluBI	AGCT	8 cut(s)	77, 239, 308, 719, 733, 965, 1019, 1119
AluI	AGCT	8 cut(s)	77, 239, 308, 719, 733, 965, 1019, 1119
Alw26I	GTCTC	2 cut(s)	665, 1797
Ama87I	CYCGRG	1 cut(s)	1582
AoxI	GGCC	6 cut(s)	132, 336, 1047, 1213, 1429, 1565
ApeKI	GCWGC	7 cut(s)	96, 197, 239, 467, 719, 768, 971
ApoI	RAATTY	3 cut(s)	33, 611, 1607
ArsI	GACNNNNNNTTYG	2 cut(s)	1352, 1384
AsiGI	ACCGGT	1 cut(s)	1481
Asp700I	GAANNNNTTC	3 cut(s)	381, 458, 1175
Asp718I	GGTACC	1 cut(s)	1352
AspA2I	CCTAGG	1 cut(s)	604
AspLEI	GCGC	1 cut(s)	215
AspS9I	GGNCC	4 cut(s)	1085, 1095, 1214, 1700
AsuC2I	CCSGG	3 cut(s)	1090, 1704, 1785
AsuHPI	GGTGA	4 cut(s)	277, 971, 1126, 1385
AvaI	CYCGRG	1 cut(s)	1582
AvaII	GGWCC	3 cut(s)	1085, 1095, 1700
AvrII	CCTAGG	1 cut(s)	604
BalI	TGGCCA	1 cut(s)	1049
BanI	GGYRCC	1 cut(s)	1352
BbsI	GAAGAC	3 cut(s)	374, 519, 579
BbvI	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 958
BccI	CCATC	5 cut(s)	491, 608, 926, 1076, 1121
BceAI	ACGGC	3 cut(s)	119, 1444, 1552
BcgI	CGANNNNNNTGC	2 cut(s)	1301, 1335
BciT130I	CCWGG	5 cut(s)	188, 665, 992, 1142, 1841
BciVI	GTATCC	1 cut(s)	1733
BclI	TGATCA	1 cut(s)	1101
BcnI	CCSGG	3 cut(s)	1090, 1704, 1785
BcoDI	GTCTC	2 cut(s)	665, 1797
BfaI	CTAG	4 cut(s)	605, 1640, 1664, 1855
BfmI	CTRYAG	3 cut(s)	720, 1276, 1501
BfoI	RGCGCY	1 cut(s)	216
BfrI	CTTAAG	2 cut(s)	449, 518
BfuI	GTATCC	1 cut(s)	1733
BisI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 972, 1565
BlnI	CCTAGG	1 cut(s)	604
BlsI	GCNGC	9 cut(s)	98, 199, 241, 274, 469, 721, 770, 973, 1566
Bme1390I	CCNGG	8 cut(s)	188, 665, 992, 1090, 1142, 1704, 1785, 1841
Bme18I	GGWCC	3 cut(s)	1085, 1095, 1700
BmeT110I	CYCGRG	1 cut(s)	1582
BmgT120I	GGNCC	4 cut(s)	1085, 1095, 1214, 1700
BmiI	GGNNCC	5 cut(s)	713, 1087, 1216, 1354, 1701
BmrFI	CCNGG	8 cut(s)	188, 665, 992, 1090, 1142, 1704, 1785, 1841
BmrI	ACTGGG	1 cut(s)	336
BmsI	GCATC	2 cut(s)	1140, 1234
BmuI	ACTGGG	1 cut(s)	336
BpiI	GAAGAC	3 cut(s)	374, 519, 579
Bpu10I	CCTNAGC	1 cut(s)	574
BpuMI	CCSGG	3 cut(s)	1090, 1704, 1785
Bsa29I	ATCGAT	1 cut(s)	258
BsaAI	YACGTR	1 cut(s)	1309
BsaHI	GRCGYC	2 cut(s)	780, 1287
BsaI	GGTCTC	1 cut(s)	1797
BsaJI	CCNNGG	7 cut(s)	129, 604, 991, 1088, 1140, 1568, 1840
BsaWI	WCCGGW	2 cut(s)	1349, 1481
BsaXI	ACNNNNNCTCC	2 cut(s)	48, 78
Bsc4I	CCNNNNNNNGG	5 cut(s)	605, 1427, 1519, 1611, 1722
Bse118I	RCCGGY	3 cut(s)	1211, 1481, 1631
Bse1I	ACTGG	4 cut(s)	331, 461, 651, 1249
Bse3DI	GCAATG	4 cut(s)	150, 358, 546, 1197
BseBI	CCWGG	5 cut(s)	188, 665, 992, 1142, 1841
BseCI	ATCGAT	1 cut(s)	258
BseDI	CCNNGG	7 cut(s)	129, 604, 991, 1088, 1140, 1568, 1840
BseGI	GGATG	5 cut(s)	42, 600, 673, 691, 1389
BseLI	CCNNNNNNNGG	5 cut(s)	605, 1427, 1519, 1611, 1722
BseMI	GCAATG	4 cut(s)	150, 358, 546, 1197
BseNI	ACTGG	4 cut(s)	331, 461, 651, 1249
BseX3I	CGGCCG	1 cut(s)	1565
BseXI	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 958
BseYI	CCCAGC	2 cut(s)	715, 1019
Bsh1236I	CGCG	1 cut(s)	1823
Bsh1285I	CGRYCG	1 cut(s)	1568
BshFI	GGCC	6 cut(s)	134, 338, 1049, 1215, 1431, 1567
BshNI	GGYRCC	1 cut(s)	1352
BshTI	ACCGGT	1 cut(s)	1481
BshVI	ATCGAT	1 cut(s)	258
BsiEI	CGRYCG	1 cut(s)	1568
BsiHKCI	CYCGRG	1 cut(s)	1582
BsiSI	CCGG	7 cut(s)	1090, 1212, 1350, 1482, 1632, 1703, 1784
BslFI	GGGAC	3 cut(s)	1496, 1628, 1666
BslI	CCNNNNNNNGG	5 cut(s)	605, 1427, 1519, 1611, 1722
BsmAI	GTCTC	2 cut(s)	665, 1797
BsmBI	CGTCTC	1 cut(s)	665
BsmFI	GGGAC	3 cut(s)	1496, 1628, 1666
BsmI	GAATGC	1 cut(s)	179
BsnI	GGCC	6 cut(s)	134, 338, 1049, 1215, 1431, 1567
Bso31I	GGTCTC	1 cut(s)	1797
BsoBI	CYCGRG	1 cut(s)	1582
Bsp1407I	TGTACA	1 cut(s)	1068
Bsp143I	GATC	2 cut(s)	445, 1101
BspACI	CCGC	4 cut(s)	272, 1471, 1564, 1823
BspANI	GGCC	6 cut(s)	134, 338, 1049, 1215, 1431, 1567
BspDI	ATCGAT	1 cut(s)	258
BspFNI	CGCG	1 cut(s)	1823
BspLI	GGNNCC	5 cut(s)	713, 1087, 1216, 1354, 1701
BspMAI	CTGCAG	3 cut(s)	724, 1280, 1505
BspQI	GCTCTTC	1 cut(s)	1776
BspT107I	GGYRCC	1 cut(s)	1352
BspTI	CTTAAG	2 cut(s)	449, 518
BspTNI	GGTCTC	1 cut(s)	1797
BsrDI	GCAATG	4 cut(s)	150, 358, 546, 1197
BsrFI	RCCGGY	3 cut(s)	1211, 1481, 1631
BsrGI	TGTACA	1 cut(s)	1068
BsrI	ACTGG	4 cut(s)	331, 461, 651, 1249
BssAI	RCCGGY	3 cut(s)	1211, 1481, 1631
BssECI	CCNNGG	7 cut(s)	129, 604, 991, 1088, 1140, 1568, 1840
BssMI	GATC	2 cut(s)	445, 1101
BssNAI	GTATAC	1 cut(s)	1829
BssNI	GRCGYC	2 cut(s)	780, 1287
BssT1I	CCWWGG	2 cut(s)	129, 604
Bst1107I	GTATAC	1 cut(s)	1829
Bst2UI	CCWGG	5 cut(s)	188, 665, 992, 1142, 1841
Bst4CI	ACNGT	7 cut(s)	638, 1045, 1340, 1357, 1387, 1591, 1674
Bst6I	CTCTTC	1 cut(s)	1776
BstACI	GRCGYC	2 cut(s)	780, 1287
BstAFI	CTTAAG	2 cut(s)	449, 518
BstAPI	GCANNNNNTGC	1 cut(s)	1688
BstAUI	TGTACA	1 cut(s)	1068
BstBAI	YACGTR	1 cut(s)	1309
BstC8I	GCNNGC	2 cut(s)	1213, 1314
BstDEI	CTNAG	1 cut(s)	574
BstDSI	CCRYGG	1 cut(s)	1568
BstF5I	GGATG	5 cut(s)	42, 600, 673, 691, 1389
BstFNI	CGCG	1 cut(s)	1823
BstH2I	RGCGCY	1 cut(s)	216
BstHHI	GCGC	1 cut(s)	215
BstKTI	GATC	2 cut(s)	448, 1104
BstMAI	GTCTC	2 cut(s)	665, 1797
BstMBI	GATC	2 cut(s)	445, 1101
BstMCI	CGRYCG	1 cut(s)	1568
BstMWI	GCNNNNNNNGC	8 cut(s)	102, 140, 183, 197, 923, 971, 1116, 1688
BstNI	CCWGG	5 cut(s)	188, 665, 992, 1142, 1841
BstNSI	RCATGY	1 cut(s)	1075
BstSCI	CCNGG	8 cut(s)	186, 663, 990, 1088, 1140, 1702, 1783, 1839
BstSFI	CTRYAG	3 cut(s)	720, 1276, 1501
BstUI	CGCG	1 cut(s)	1823
BstV1I	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 958
BstV2I	GAAGAC	3 cut(s)	374, 519, 579
BstXI	CCANNNNNNTGG	2 cut(s)	1486, 1635
BstZ17I	GTATAC	1 cut(s)	1829
BstZI	CGGCCG	1 cut(s)	1565
Bsu15I	ATCGAT	1 cut(s)	258
BsuI	GTATCC	1 cut(s)	1733
BsuRI	GGCC	6 cut(s)	134, 338, 1049, 1215, 1431, 1567
BsuTUI	ATCGAT	1 cut(s)	258
BtgI	CCRYGG	1 cut(s)	1568
BtsCI	GGATG	5 cut(s)	42, 600, 673, 691, 1389
BtsI	GCAGTG	2 cut(s)	1146, 1239
BtsIMutI	CAGTG	5 cut(s)	324, 469, 1146, 1239, 1670
Cac8I	GCNNGC	2 cut(s)	1213, 1314
CfoI	GCGC	1 cut(s)	215
Cfr10I	RCCGGY	3 cut(s)	1211, 1481, 1631
Cfr13I	GGNCC	4 cut(s)	1085, 1095, 1214, 1700
ClaI	ATCGAT	1 cut(s)	258
CseI	GACGC	3 cut(s)	203, 1295, 1829
Csp6I	GTAC	5 cut(s)	976, 1069, 1306, 1353, 1579
CspAI	ACCGGT	1 cut(s)	1481
CviQI	GTAC	5 cut(s)	976, 1069, 1306, 1353, 1579
DdeI	CTNAG	1 cut(s)	574
DpnI	GATC	2 cut(s)	447, 1103
DpnII	GATC	2 cut(s)	445, 1101
DraI	TTTAAA	1 cut(s)	1848
EaeI	YGGCCR	3 cut(s)	1047, 1429, 1565
EagI	CGGCCG	1 cut(s)	1565
Eam1104I	CTCTTC	1 cut(s)	1776
EarI	CTCTTC	1 cut(s)	1776
EclXI	CGGCCG	1 cut(s)	1565
Eco130I	CCWWGG	2 cut(s)	129, 604
Eco31I	GGTCTC	1 cut(s)	1797
Eco32I	GATATC	1 cut(s)	474
Eco47I	GGWCC	3 cut(s)	1085, 1095, 1700
Eco52I	CGGCCG	1 cut(s)	1565
Eco88I	CYCGRG	1 cut(s)	1582
EcoRII	CCWGG	5 cut(s)	186, 663, 990, 1140, 1839
EcoRV	GATATC	1 cut(s)	474
EcoT14I	CCWWGG	2 cut(s)	129, 604
EcoT22I	ATGCAT	2 cut(s)	179, 494
ErhI	CCWWGG	2 cut(s)	129, 604
Esp3I	CGTCTC	1 cut(s)	665
FaqI	GGGAC	3 cut(s)	1496, 1628, 1666
FauI	CCCGC	2 cut(s)	1464, 1816
FbaI	TGATCA	1 cut(s)	1101
FblI	GTMKAC	5 cut(s)	786, 837, 870, 879, 1828
Fnu4HI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 972, 1565
FokI	GGATG	5 cut(s)	29, 587, 680, 698, 1376
Fsp4HI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 972, 1565
FspBI	CTAG	4 cut(s)	605, 1640, 1664, 1855
GlaI	GCGC	1 cut(s)	214
GluI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 972, 1565
GsaI	CCCAGC	2 cut(s)	719, 1023
HaeII	RGCGCY	1 cut(s)	216
HaeIII	GGCC	6 cut(s)	134, 338, 1049, 1215, 1431, 1567
HapII	CCGG	7 cut(s)	1090, 1212, 1350, 1482, 1632, 1703, 1784
HgaI	GACGC	3 cut(s)	203, 1295, 1829
HhaI	GCGC	1 cut(s)	215
Hin1I	GRCGYC	2 cut(s)	780, 1287
Hin6I	GCGC	1 cut(s)	213
HinP1I	GCGC	1 cut(s)	213
HincII	GTYRAC	5 cut(s)	787, 838, 871, 880, 1360
HindII	GTYRAC	5 cut(s)	787, 838, 871, 880, 1360
HindIII	AAGCTT	1 cut(s)	1117
HpaII	CCGG	7 cut(s)	1090, 1212, 1350, 1482, 1632, 1703, 1784
HphI	GGTGA	4 cut(s)	277, 971, 1126, 1385
Hpy188I	TCNGA	6 cut(s)	236, 289, 1203, 1244, 1255, 1606
Hpy188III	TCNNGA	5 cut(s)	482, 1393, 1495, 1543, 1584
Hpy99I	CGWCG	9 cut(s)	124, 660, 782, 785, 788, 797, 800, 848, 881
HpyAV	CCTTC	4 cut(s)	4, 349, 1276, 1643
HpyCH4III	ACNGT	7 cut(s)	638, 1045, 1340, 1357, 1387, 1591, 1674
HpyCH4IV	ACGT	3 cut(s)	780, 876, 1308
HpyF10VI	GCNNNNNNNGC	8 cut(s)	102, 140, 183, 197, 923, 971, 1116, 1688
HpyF3I	CTNAG	1 cut(s)	574
HpySE526I	ACGT	3 cut(s)	780, 876, 1308
Hsp92I	GRCGYC	2 cut(s)	780, 1287
HspAI	GCGC	1 cut(s)	213
KpnI	GGTACC	1 cut(s)	1356
KroI	GCCGGC	1 cut(s)	1211
KroNI	GCCGGC	1 cut(s)	1213
Ksp22I	TGATCA	1 cut(s)	1101
Kzo9I	GATC	2 cut(s)	445, 1101
LguI	GCTCTTC	1 cut(s)	1776
LmnI	GCTCC	3 cut(s)	56, 549, 601
Lsp1109I	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 958
LweI	GCATC	2 cut(s)	1140, 1234
MaeI	CTAG	4 cut(s)	605, 1640, 1664, 1855
MaeII	ACGT	3 cut(s)	780, 876, 1308
MaeIII	GTNAC	5 cut(s)	904, 1039, 1373, 1444, 1711
MalI	GATC	2 cut(s)	447, 1103
MboI	GATC	2 cut(s)	445, 1101
MfeI	CAATTG	2 cut(s)	579, 954
MlsI	TGGCCA	1 cut(s)	1049
MluCI	AATT	8 cut(s)	33, 579, 611, 954, 1325, 1607, 1775, 1789
MluNI	TGGCCA	1 cut(s)	1049
MlyI	GAGTC	5 cut(s)	843, 876, 1254, 1370, 1610
MmeI	TCCRAC	4 cut(s)	34, 342, 1233, 1648
MnlI	CCTC	5 cut(s)	433, 529, 1086, 1407, 1759
Mox20I	TGGCCA	1 cut(s)	1049
Mph1103I	ATGCAT	2 cut(s)	179, 494
MroNI	GCCGGC	1 cut(s)	1211
MroXI	GAANNNNTTC	3 cut(s)	381, 458, 1175
MscI	TGGCCA	1 cut(s)	1049
MseI	TTAA	3 cut(s)	450, 519, 1847
MslI	CAYNNNNRTG	2 cut(s)	47, 1163
Msp20I	TGGCCA	1 cut(s)	1049
MspA1I	CMGCKG	1 cut(s)	719
MspCI	CTTAAG	2 cut(s)	449, 518
MspI	CCGG	7 cut(s)	1090, 1212, 1350, 1482, 1632, 1703, 1784
MspR9I	CCNGG	8 cut(s)	188, 665, 992, 1090, 1142, 1704, 1785, 1841
MunI	CAATTG	2 cut(s)	579, 954
Mva1269I	GAATGC	1 cut(s)	179
MvaI	CCWGG	5 cut(s)	188, 665, 992, 1142, 1841
MvnI	CGCG	1 cut(s)	1823
MwoI	GCNNNNNNNGC	8 cut(s)	102, 140, 183, 197, 923, 971, 1116, 1688
NaeI	GCCGGC	1 cut(s)	1213
NciI	CCSGG	3 cut(s)	1090, 1704, 1785
NdeII	GATC	2 cut(s)	445, 1101
NgoMIV	GCCGGC	1 cut(s)	1211
NlaIV	GGNNCC	5 cut(s)	713, 1087, 1216, 1354, 1701
NmuCI	GTSAC	2 cut(s)	1373, 1444
NsiI	ATGCAT	2 cut(s)	179, 494
NspI	RCATGY	1 cut(s)	1075
PaeR7I	CTCGAG	1 cut(s)	1582
PciI	ACATGT	1 cut(s)	1071
PciSI	GCTCTTC	1 cut(s)	1776
PcsI	WCGNNNNNNNCGW	7 cut(s)	783, 789, 792, 834, 843, 867, 876
PctI	GAATGC	1 cut(s)	179
PdiI	GCCGGC	1 cut(s)	1213
PdmI	GAANNNNTTC	3 cut(s)	381, 458, 1175
PfeI	GAWTC	8 cut(s)	205, 365, 485, 502, 987, 1054, 1539, 1546
PflFI	GACNNNGTC	2 cut(s)	782, 875
PfoI	TCCNGGA	1 cut(s)	663
PinAI	ACCGGT	1 cut(s)	1481
PkrI	GCNGC	9 cut(s)	98, 199, 241, 274, 469, 721, 770, 973, 1566
PleI	GAGTC	5 cut(s)	842, 875, 1253, 1370, 1609
PpsI	GAGTC	5 cut(s)	842, 875, 1253, 1370, 1609
Ppu21I	YACGTR	1 cut(s)	1309
PscI	ACATGT	1 cut(s)	1071
Psp6I	CCWGG	5 cut(s)	186, 663, 990, 1140, 1839
PspFI	CCCAGC	2 cut(s)	715, 1019
PspGI	CCWGG	5 cut(s)	186, 663, 990, 1140, 1839
PspN4I	GGNNCC	5 cut(s)	713, 1087, 1216, 1354, 1701
PspPI	GGNCC	4 cut(s)	1085, 1095, 1214, 1700
PstI	CTGCAG	3 cut(s)	724, 1280, 1505
PsyI	GACNNNGTC	2 cut(s)	782, 875
PvuII	CAGCTG	1 cut(s)	719
RsaI	GTAC	5 cut(s)	977, 1070, 1307, 1354, 1580
RsaNI	GTAC	5 cut(s)	976, 1069, 1306, 1353, 1579
RseI	CAYNNNNRTG	2 cut(s)	47, 1163
SalI	GTCGAC	4 cut(s)	785, 836, 869, 878
SapI	GCTCTTC	1 cut(s)	1776
SaqAI	TTAA	3 cut(s)	450, 519, 1847
SatI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 972, 1565
Sau3AI	GATC	2 cut(s)	445, 1101
Sau96I	GGNCC	4 cut(s)	1085, 1095, 1214, 1700
SchI	GAGTC	5 cut(s)	843, 876, 1254, 1370, 1610
ScrFI	CCNGG	8 cut(s)	188, 665, 992, 1090, 1142, 1704, 1785, 1841
SfaNI	GCATC	2 cut(s)	1140, 1234
SfcI	CTRYAG	3 cut(s)	720, 1276, 1501
Sfr274I	CTCGAG	1 cut(s)	1582
SinI	GGWCC	3 cut(s)	1085, 1095, 1700
SlaI	CTCGAG	1 cut(s)	1582
SmiMI	CAYNNNNRTG	2 cut(s)	47, 1163
SmlI	CTYRAG	3 cut(s)	449, 518, 1582
SmoI	CTYRAG	3 cut(s)	449, 518, 1582
Sse9I	AATT	8 cut(s)	33, 579, 611, 954, 1325, 1607, 1775, 1789
SsiI	CCGC	4 cut(s)	272, 1471, 1564, 1823
SspI	AATATT	1 cut(s)	389
SspMI	CTAG	4 cut(s)	605, 1640, 1664, 1855
StyD4I	CCNGG	8 cut(s)	186, 663, 990, 1088, 1140, 1702, 1783, 1839
StyI	CCWWGG	2 cut(s)	129, 604
TaaI	ACNGT	7 cut(s)	638, 1045, 1340, 1357, 1387, 1591, 1674
TaiI	ACGT	3 cut(s)	783, 879, 1311
TaqI	TCGA	8 cut(s)	119, 258, 375, 786, 837, 870, 879, 1583
TasI	AATT	8 cut(s)	33, 579, 611, 954, 1325, 1607, 1775, 1789
TatI	WGTACW	2 cut(s)	1068, 1578
TauI	GCSGC	2 cut(s)	275, 1567
TfiI	GAWTC	8 cut(s)	205, 365, 485, 502, 987, 1054, 1539, 1546
Tru1I	TTAA	3 cut(s)	450, 519, 1847
Tru9I	TTAA	3 cut(s)	450, 519, 1847
TscAI	CASTG	5 cut(s)	331, 469, 1153, 1239, 1677
TseFI	GTSAC	2 cut(s)	1373, 1444
TseI	GCWGC	7 cut(s)	96, 197, 239, 467, 719, 768, 971
Tsp45I	GTSAC	2 cut(s)	1373, 1444
TspDTI	ATGAA	7 cut(s)	443, 545, 641, 702, 1147, 1168, 1354
TspGWI	ACGGA	2 cut(s)	698, 1769
TspRI	CASTG	5 cut(s)	331, 469, 1153, 1239, 1677
Tth111I	GACNNNGTC	2 cut(s)	782, 875
Vha464I	CTTAAG	2 cut(s)	449, 518
VpaK11BI	GGWCC	3 cut(s)	1085, 1095, 1700
XapI	RAATTY	3 cut(s)	33, 611, 1607
XceI	RCATGY	1 cut(s)	1075
XcmI	CCANNNNNNNNNTGG	2 cut(s)	331, 919
XhoI	CTCGAG	1 cut(s)	1582
XmaJI	CCTAGG	1 cut(s)	604
XmiI	GTMKAC	5 cut(s)	786, 837, 870, 879, 1828
XmnI	GAANNNNTTC	3 cut(s)	381, 458, 1175
XspI	CTAG	4 cut(s)	605, 1640, 1664, 1855
ZraI	GACGTC	1 cut(s)	781
Zsp2I	ATGCAT	2 cut(s)	179, 494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.