Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0003768

Physical Location & Seq

Genomic Coordinates Forward (+)

55515831 .. 55517842

Gene Structure 2012 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG295800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1905 bp

ATGGAAAAGAAGGGAAAGGCAATAGTAGGTGGAATTTCAATCATCCTTGTGGTTGGAGCAGTAGTTGGTTGTGTAGCTGTTGTTTCACATAATCGCAGCAAAGGCAAGGATAGGCTTTCGACGAGTTCCAAGGCCGTCAATGCAATGTGTGAACCAACTGATTACAAGGAAGAATGCATTGACAGCCTGGCCGACGCTGCCAAGAATCAAAGCGCCACCCCAAAAGAACTTATCCGAGCTGCCATTGATTACACAATCGATAAGGTGAAAGCGGCAATAGAGAAATCAGATAGCATTATCAAAGAAGCTAAAAATGTCAGTTCCACTGGAAAAATGGCCTTCGAGGATTGCAATGACTTGTTGGAATCTTCAATCGAAAGTCTTCAATATTCGCTTTTGACACTTGTTGATACTGAAATGCAAAACATGAATAATAAAGAGGCTGATCTTAAGAACTGGTTCAGTGCTGCGATATCATTTCAGGAATCATGCATTGATGGGATTCCATACGAAGACCTTAAGAACCAAATGAACGAGGCAATGCTCCATGCTTCACAACTGACCAGCAACGCCTTAGCAATTGTGTCTTCCCTTGCTCCCATCCTAGGCAAATTCAATATCTCCATGAAAACTGACAGTAAAAGTTCAACCAGTCGTCGTCTCCTGGATGTAGAAATGGAAACGGATGAACATGGCACTTTTCCAGCATGGTTCCCAGCTGCAGATAGGAAGCTATTCGCACACCACAACAAGAACAATAACAATAGCAGCAACAACAACAACGACAACGACAACAAGAACAATAACAATAGCAGCAACAACAACAACGACAACGACAACAACAACAACGACAACAACAACAACGACGACGACAACAACGACAACAACGACGACAACAACAACAACAACAACAACAACAACGACGACGACAACAACAACGACAACGGGAGAGTTACACCCAACGCAGTAGTGGCAAAAGATGGGAGTGGACAATACAAAACAATTGGTGAAGCTCTTGATGCGTACCCAAAGGGATTCCAGGGAAGATATGTTATATATGTGAAAGCTGGGATTTACAATGAGTATGTTACTGTGGCCAAGAATCAGATCAATGTGTACATGTATGGAGATGGACCCCGGAGGACCATGATCACAGGCAGTAAAAGCTTTGCTGATGGCATCACCACCCAGGACACTGCCACATTCATCGTAATGGGGCATGGGTTCATTGGTAGAGCAATGGGATTTCAGAACACAGCCGGCCCCGAAAAGCATCAGGCAGTGGCACTCCGAGTCCAGTCGGAAATGTCAGCCTTCTTCAACTGCAGAATGGACGCCTACCAAGACACATTGTACGTGCAAGCATATCGCCAATTTTATCGCAACTGTGTCATATCCGGTACCGTTGACTTCATTTTTGGTGACTCATCAACAGTCATCCAGAATAGTTTACTCATTGTGAGGAAGCCTATGGACGGCCAAAAGAACATTGTGACTGCTCATGGAAGAAAAGATAAGCGGGAAACCACTGGTCTGGTTATCCAGAACTGCAGGATAGTCCCAGAACAGGCTTTGTTCCCAATGAGATTCCAGATTCAGTCATACTTGGGGCGGCCGTGGAAGATGTACTCGAGAACAGTTATCATGGAGTCCGAATTTGGGGACTTTATCCAACCAGCCGGTTGGCTAGAATGGGAAGGGACTTTTGGACTAGACACACTGTATTATGCAGAGTATGCAAATCGGGGTCCGGGTGCTGTAACCACAGGAAGGGTAAAATGGAAAGGATACCATGTGATTACGGACAGAAATGAGGCTCTTCAATTCACTCCGGGTAATTTTCTAACAGGAGACCAATGGTTGAAAGACACGGGTGTACCTTACTTCCTGGGTTTAAAAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

634

Amino Acids

70.49

Weight (kDa)

5.35

Isoelectric Point (pI)

28.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	45 - 194	1.8e-29	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	321 - 618	1.2e-135	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003768)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47030 AT2G47040 AT2G47050 AT3G62170 AT3G62180 AT3G62180
fragaria_vesca	FvH4_1g26360 FvH4_4g21500
malus_domestica	MD16G1136400.v1.1 MD17G1283800.v1.1
prunus_persica	Prupe.1G105400_v2.0.a1 Prupe.3G003700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0486131 RchiOBHm_Chr4g0427511
rosa_laevigata	RLG00000007232 RLG00000023101
rosa_multiflora	Rmu_sc0001006.1_g000002 Rmu_sc0009627.1_g000001 Rmu_sc0017895.1_g000001 Rmu_sc0017895.1_g000002
rosa_roxburghii	Rroxscaffold_5G00369150 Rroxscaffold_6G00396030
rosa_rugosa	Rorug03G0223700 Rorug04G0218200
rosa_samantha	Rh3AG273500 Rh3BG308600 Rh3CG307100 Rh3DG303400 Rh4AG274700 Rh4BG280500 Rh4CG295800 Rh4DG277900
rosa_wichuraiana	Rw3G024240 Rw4G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1400
AccB1I	GGYRCC	1 cut(s)	1400
AciI	CCGC	3 cut(s)	272, 1519, 1612
AcoI	YGGCCR	4 cut(s)	189, 1095, 1477, 1613
AcsI	RAATTY	3 cut(s)	33, 611, 1655
AcyI	GRCGYC	1 cut(s)	1335
AfaI	GTAC	6 cut(s)	1025, 1118, 1355, 1402, 1628, 1878
AfiI	CCNNNNNNNGG	5 cut(s)	605, 1475, 1567, 1659, 1770
AflII	CTTAAG	2 cut(s)	449, 518
AflIII	ACRYGT	1 cut(s)	1119
AgsI	TTSAA	8 cut(s)	39, 372, 386, 616, 648, 1321, 1823, 1864
AjnI	CCWGG	5 cut(s)	186, 663, 1038, 1188, 1887
AjuI	GAANNNNNNNTTGG	2 cut(s)	1689, 1721
AluBI	AGCT	8 cut(s)	77, 239, 308, 719, 733, 1013, 1067, 1167
AluI	AGCT	8 cut(s)	77, 239, 308, 719, 733, 1013, 1067, 1167
Alw26I	GTCTC	2 cut(s)	665, 1845
Ama87I	CYCGRG	1 cut(s)	1630
AoxI	GGCC	7 cut(s)	132, 189, 336, 1095, 1261, 1477, 1613
ApeKI	GCWGC	7 cut(s)	96, 197, 239, 467, 719, 768, 813
ApoI	RAATTY	3 cut(s)	33, 611, 1655
ArsI	GACNNNNNNTTYG	2 cut(s)	1400, 1432
Asp700I	GAANNNNTTC	3 cut(s)	381, 458, 734
Asp718I	GGTACC	1 cut(s)	1400
AspA2I	CCTAGG	1 cut(s)	604
AspLEI	GCGC	1 cut(s)	215
AspS9I	GGNCC	4 cut(s)	1133, 1143, 1262, 1748
AsuC2I	CCSGG	3 cut(s)	1138, 1752, 1833
AsuHPI	GGTGA	4 cut(s)	277, 1019, 1174, 1433
AvaI	CYCGRG	1 cut(s)	1630
AvaII	GGWCC	3 cut(s)	1133, 1143, 1748
AvrII	CCTAGG	1 cut(s)	604
BaeI	ACNNNNGTAYC	2 cut(s)	1860, 1893
BalI	TGGCCA	1 cut(s)	1097
BanI	GGYRCC	1 cut(s)	1400
BbsI	GAAGAC	3 cut(s)	374, 519, 579
BbvI	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 825
BccI	CCATC	5 cut(s)	491, 608, 974, 1124, 1169
BceAI	ACGGC	3 cut(s)	119, 1492, 1600
BcgI	CGANNNNNNTGC	2 cut(s)	1349, 1383
BciT130I	CCWGG	5 cut(s)	188, 665, 1040, 1190, 1889
BciVI	GTATCC	1 cut(s)	1781
BclI	TGATCA	1 cut(s)	1149
BcnI	CCSGG	3 cut(s)	1138, 1752, 1833
BcoDI	GTCTC	2 cut(s)	665, 1845
BfaI	CTAG	4 cut(s)	605, 1688, 1712, 1903
BfmI	CTRYAG	3 cut(s)	720, 1324, 1549
BfoI	RGCGCY	1 cut(s)	216
BfrI	CTTAAG	2 cut(s)	449, 518
BfuI	GTATCC	1 cut(s)	1781
BisI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 814, 1613
BlnI	CCTAGG	1 cut(s)	604
BlsI	GCNGC	9 cut(s)	98, 199, 241, 274, 469, 721, 770, 815, 1614
Bme1390I	CCNGG	8 cut(s)	188, 665, 1040, 1138, 1190, 1752, 1833, 1889
Bme18I	GGWCC	3 cut(s)	1133, 1143, 1748
BmeT110I	CYCGRG	1 cut(s)	1630
BmgT120I	GGNCC	4 cut(s)	1133, 1143, 1262, 1748
BmiI	GGNNCC	5 cut(s)	713, 1135, 1264, 1402, 1749
BmrFI	CCNGG	8 cut(s)	188, 665, 1040, 1138, 1190, 1752, 1833, 1889
BmsI	GCATC	3 cut(s)	1009, 1188, 1282
BpiI	GAAGAC	3 cut(s)	374, 519, 579
Bpu10I	CCTNAGC	1 cut(s)	574
BpuMI	CCSGG	3 cut(s)	1138, 1752, 1833
Bsa29I	ATCGAT	1 cut(s)	258
BsaAI	YACGTR	1 cut(s)	1357
BsaHI	GRCGYC	1 cut(s)	1335
BsaI	GGTCTC	1 cut(s)	1845
BsaJI	CCNNGG	7 cut(s)	129, 604, 1039, 1136, 1188, 1616, 1888
BsaWI	WCCGGW	1 cut(s)	1397
BsaXI	ACNNNNNCTCC	2 cut(s)	48, 78
Bsc4I	CCNNNNNNNGG	5 cut(s)	605, 1475, 1567, 1659, 1770
Bse118I	RCCGGY	2 cut(s)	1259, 1679
Bse1I	ACTGG	5 cut(s)	331, 461, 651, 1297, 1534
Bse3DI	GCAATG	4 cut(s)	150, 358, 546, 1245
BseBI	CCWGG	5 cut(s)	188, 665, 1040, 1190, 1889
BseCI	ATCGAT	1 cut(s)	258
BseDI	CCNNGG	7 cut(s)	129, 604, 1039, 1136, 1188, 1616, 1888
BseGI	GGATG	5 cut(s)	42, 600, 673, 691, 1437
BseLI	CCNNNNNNNGG	5 cut(s)	605, 1475, 1567, 1659, 1770
BseMI	GCAATG	4 cut(s)	150, 358, 546, 1245
BseNI	ACTGG	5 cut(s)	331, 461, 651, 1297, 1534
BseX3I	CGGCCG	1 cut(s)	1613
BseXI	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 825
BseYI	CCCAGC	2 cut(s)	715, 1067
Bsh1285I	CGRYCG	1 cut(s)	1616
BshFI	GGCC	7 cut(s)	134, 191, 338, 1097, 1263, 1479, 1615
BshNI	GGYRCC	1 cut(s)	1400
BshVI	ATCGAT	1 cut(s)	258
BsiEI	CGRYCG	1 cut(s)	1616
BsiHKCI	CYCGRG	1 cut(s)	1630
BsiSI	CCGG	6 cut(s)	1138, 1260, 1398, 1680, 1751, 1832
BslFI	GGGAC	3 cut(s)	1544, 1676, 1714
BslI	CCNNNNNNNGG	5 cut(s)	605, 1475, 1567, 1659, 1770
BsmAI	GTCTC	2 cut(s)	665, 1845
BsmBI	CGTCTC	1 cut(s)	665
BsmFI	GGGAC	3 cut(s)	1544, 1676, 1714
BsmI	GAATGC	1 cut(s)	179
BsnI	GGCC	7 cut(s)	134, 191, 338, 1097, 1263, 1479, 1615
Bso31I	GGTCTC	1 cut(s)	1845
BsoBI	CYCGRG	1 cut(s)	1630
Bsp1407I	TGTACA	1 cut(s)	1116
Bsp143I	GATC	3 cut(s)	445, 1107, 1149
BspACI	CCGC	3 cut(s)	272, 1519, 1612
BspANI	GGCC	7 cut(s)	134, 191, 338, 1097, 1263, 1479, 1615
BspDI	ATCGAT	1 cut(s)	258
BspLI	GGNNCC	5 cut(s)	713, 1135, 1264, 1402, 1749
BspMAI	CTGCAG	3 cut(s)	724, 1328, 1553
BspQI	GCTCTTC	1 cut(s)	1824
BspT107I	GGYRCC	1 cut(s)	1400
BspTI	CTTAAG	2 cut(s)	449, 518
BspTNI	GGTCTC	1 cut(s)	1845
BsrDI	GCAATG	4 cut(s)	150, 358, 546, 1245
BsrFI	RCCGGY	2 cut(s)	1259, 1679
BsrGI	TGTACA	1 cut(s)	1116
BsrI	ACTGG	5 cut(s)	331, 461, 651, 1297, 1534
BssAI	RCCGGY	2 cut(s)	1259, 1679
BssECI	CCNNGG	7 cut(s)	129, 604, 1039, 1136, 1188, 1616, 1888
BssMI	GATC	3 cut(s)	445, 1107, 1149
BssNI	GRCGYC	1 cut(s)	1335
BssT1I	CCWWGG	2 cut(s)	129, 604
Bst2UI	CCWGG	5 cut(s)	188, 665, 1040, 1190, 1889
Bst4CI	ACNGT	7 cut(s)	638, 1093, 1388, 1405, 1435, 1639, 1722
Bst6I	CTCTTC	1 cut(s)	1824
BstACI	GRCGYC	1 cut(s)	1335
BstAFI	CTTAAG	2 cut(s)	449, 518
BstAPI	GCANNNNNTGC	1 cut(s)	1736
BstAUI	TGTACA	1 cut(s)	1116
BstBAI	YACGTR	1 cut(s)	1357
BstC8I	GCNNGC	2 cut(s)	1261, 1362
BstDEI	CTNAG	1 cut(s)	574
BstDSI	CCRYGG	1 cut(s)	1616
BstF5I	GGATG	5 cut(s)	42, 600, 673, 691, 1437
BstH2I	RGCGCY	1 cut(s)	216
BstHHI	GCGC	1 cut(s)	215
BstKTI	GATC	3 cut(s)	448, 1110, 1152
BstMAI	GTCTC	2 cut(s)	665, 1845
BstMBI	GATC	3 cut(s)	445, 1107, 1149
BstMCI	CGRYCG	1 cut(s)	1616
BstMWI	GCNNNNNNNGC	8 cut(s)	102, 140, 183, 197, 971, 1019, 1164, 1736
BstNI	CCWGG	5 cut(s)	188, 665, 1040, 1190, 1889
BstNSI	RCATGY	1 cut(s)	1123
BstSCI	CCNGG	8 cut(s)	186, 663, 1038, 1136, 1188, 1750, 1831, 1887
BstSFI	CTRYAG	3 cut(s)	720, 1324, 1549
BstV1I	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 825
BstV2I	GAAGAC	3 cut(s)	374, 519, 579
BstXI	CCANNNNNNTGG	2 cut(s)	1534, 1683
BstZI	CGGCCG	1 cut(s)	1613
Bsu15I	ATCGAT	1 cut(s)	258
BsuI	GTATCC	1 cut(s)	1781
BsuRI	GGCC	7 cut(s)	134, 191, 338, 1097, 1263, 1479, 1615
BsuTUI	ATCGAT	1 cut(s)	258
BtgI	CCRYGG	1 cut(s)	1616
BtsCI	GGATG	5 cut(s)	42, 600, 673, 691, 1437
BtsI	GCAGTG	2 cut(s)	1194, 1287
BtsIMutI	CAGTG	6 cut(s)	324, 469, 1194, 1287, 1527, 1718
Cac8I	GCNNGC	2 cut(s)	1261, 1362
CfoI	GCGC	1 cut(s)	215
Cfr10I	RCCGGY	2 cut(s)	1259, 1679
Cfr13I	GGNCC	4 cut(s)	1133, 1143, 1262, 1748
ClaI	ATCGAT	1 cut(s)	258
CseI	GACGC	2 cut(s)	203, 1343
Csp6I	GTAC	6 cut(s)	1024, 1117, 1354, 1401, 1627, 1877
CviQI	GTAC	6 cut(s)	1024, 1117, 1354, 1401, 1627, 1877
DdeI	CTNAG	1 cut(s)	574
DpnI	GATC	3 cut(s)	447, 1109, 1151
DpnII	GATC	3 cut(s)	445, 1107, 1149
DraI	TTTAAA	1 cut(s)	1896
EaeI	YGGCCR	4 cut(s)	189, 1095, 1477, 1613
EagI	CGGCCG	1 cut(s)	1613
Eam1104I	CTCTTC	1 cut(s)	1824
EarI	CTCTTC	1 cut(s)	1824
EclXI	CGGCCG	1 cut(s)	1613
Eco130I	CCWWGG	2 cut(s)	129, 604
Eco31I	GGTCTC	1 cut(s)	1845
Eco32I	GATATC	1 cut(s)	474
Eco47I	GGWCC	3 cut(s)	1133, 1143, 1748
Eco52I	CGGCCG	1 cut(s)	1613
Eco88I	CYCGRG	1 cut(s)	1630
EcoRII	CCWGG	5 cut(s)	186, 663, 1038, 1188, 1887
EcoRV	GATATC	1 cut(s)	474
EcoT14I	CCWWGG	2 cut(s)	129, 604
EcoT22I	ATGCAT	2 cut(s)	179, 494
ErhI	CCWWGG	2 cut(s)	129, 604
Esp3I	CGTCTC	1 cut(s)	665
FaqI	GGGAC	3 cut(s)	1544, 1676, 1714
FauI	CCCGC	1 cut(s)	1512
FbaI	TGATCA	1 cut(s)	1149
Fnu4HI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 814, 1613
FokI	GGATG	5 cut(s)	29, 587, 680, 698, 1424
Fsp4HI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 814, 1613
FspBI	CTAG	4 cut(s)	605, 1688, 1712, 1903
GlaI	GCGC	1 cut(s)	214
GluI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 814, 1613
GsaI	CCCAGC	2 cut(s)	719, 1071
HaeII	RGCGCY	1 cut(s)	216
HaeIII	GGCC	7 cut(s)	134, 191, 338, 1097, 1263, 1479, 1615
HapII	CCGG	6 cut(s)	1138, 1260, 1398, 1680, 1751, 1832
HgaI	GACGC	2 cut(s)	203, 1343
HhaI	GCGC	1 cut(s)	215
Hin1I	GRCGYC	1 cut(s)	1335
Hin6I	GCGC	1 cut(s)	213
HinP1I	GCGC	1 cut(s)	213
HincII	GTYRAC	1 cut(s)	1408
HindII	GTYRAC	1 cut(s)	1408
HindIII	AAGCTT	1 cut(s)	1165
HpaII	CCGG	6 cut(s)	1138, 1260, 1398, 1680, 1751, 1832
HphI	GGTGA	4 cut(s)	277, 1019, 1174, 1433
Hpy166II	GTNNAC	6 cut(s)	152, 989, 1117, 1408, 1451, 1877
Hpy188I	TCNGA	7 cut(s)	236, 289, 1107, 1251, 1292, 1303, 1654
Hpy188III	TCNNGA	6 cut(s)	482, 1016, 1441, 1543, 1591, 1632
Hpy8I	GTNNAC	6 cut(s)	152, 989, 1117, 1408, 1451, 1877
Hpy99I	CGWCG	8 cut(s)	124, 197, 660, 869, 872, 893, 926, 929
HpyAV	CCTTC	5 cut(s)	4, 349, 1324, 1691, 1764
HpyCH4III	ACNGT	7 cut(s)	638, 1093, 1388, 1405, 1435, 1639, 1722
HpyCH4IV	ACGT	1 cut(s)	1356
HpyF10VI	GCNNNNNNNGC	8 cut(s)	102, 140, 183, 197, 971, 1019, 1164, 1736
HpyF3I	CTNAG	1 cut(s)	574
HpySE526I	ACGT	1 cut(s)	1356
Hsp92I	GRCGYC	1 cut(s)	1335
HspAI	GCGC	1 cut(s)	213
KpnI	GGTACC	1 cut(s)	1404
KroI	GCCGGC	1 cut(s)	1259
KroNI	GCCGGC	1 cut(s)	1261
Ksp22I	TGATCA	1 cut(s)	1149
Kzo9I	GATC	3 cut(s)	445, 1107, 1149
LguI	GCTCTTC	1 cut(s)	1824
LmnI	GCTCC	3 cut(s)	56, 549, 601
Lsp1109I	GCAGC	7 cut(s)	108, 184, 226, 454, 706, 780, 825
LweI	GCATC	3 cut(s)	1009, 1188, 1282
MaeI	CTAG	4 cut(s)	605, 1688, 1712, 1903
MaeII	ACGT	1 cut(s)	1356
MaeIII	GTNAC	5 cut(s)	952, 1087, 1421, 1492, 1759
MalI	GATC	3 cut(s)	447, 1109, 1151
MboI	GATC	3 cut(s)	445, 1107, 1149
MfeI	CAATTG	2 cut(s)	579, 1002
MlsI	TGGCCA	1 cut(s)	1097
MluCI	AATT	8 cut(s)	33, 579, 611, 1002, 1373, 1655, 1823, 1837
MluNI	TGGCCA	1 cut(s)	1097
MlyI	GAGTC	3 cut(s)	1302, 1418, 1658
MmeI	TCCRAC	4 cut(s)	34, 342, 1281, 1696
MnlI	CCTC	6 cut(s)	337, 433, 529, 1134, 1455, 1807
Mox20I	TGGCCA	1 cut(s)	1097
Mph1103I	ATGCAT	2 cut(s)	179, 494
MroNI	GCCGGC	1 cut(s)	1259
MroXI	GAANNNNTTC	3 cut(s)	381, 458, 734
MscI	TGGCCA	1 cut(s)	1097
MseI	TTAA	3 cut(s)	450, 519, 1895
MslI	CAYNNNNRTG	2 cut(s)	47, 1211
Msp20I	TGGCCA	1 cut(s)	1097
MspA1I	CMGCKG	1 cut(s)	719
MspCI	CTTAAG	2 cut(s)	449, 518
MspI	CCGG	6 cut(s)	1138, 1260, 1398, 1680, 1751, 1832
MspR9I	CCNGG	8 cut(s)	188, 665, 1040, 1138, 1190, 1752, 1833, 1889
MunI	CAATTG	2 cut(s)	579, 1002
Mva1269I	GAATGC	1 cut(s)	179
MvaI	CCWGG	5 cut(s)	188, 665, 1040, 1190, 1889
MwoI	GCNNNNNNNGC	8 cut(s)	102, 140, 183, 197, 971, 1019, 1164, 1736
NaeI	GCCGGC	1 cut(s)	1261
NciI	CCSGG	3 cut(s)	1138, 1752, 1833
NdeII	GATC	3 cut(s)	445, 1107, 1149
NgoMIV	GCCGGC	1 cut(s)	1259
NlaIV	GGNNCC	5 cut(s)	713, 1135, 1264, 1402, 1749
NmuCI	GTSAC	2 cut(s)	1421, 1492
NsiI	ATGCAT	2 cut(s)	179, 494
NspI	RCATGY	1 cut(s)	1123
PaeR7I	CTCGAG	1 cut(s)	1630
PciI	ACATGT	1 cut(s)	1119
PciSI	GCTCTTC	1 cut(s)	1824
PcsI	WCGNNNNNNNCGW	2 cut(s)	876, 885
PctI	GAATGC	1 cut(s)	179
PdiI	GCCGGC	1 cut(s)	1261
PdmI	GAANNNNTTC	3 cut(s)	381, 458, 734
PfeI	GAWTC	8 cut(s)	205, 365, 485, 502, 1035, 1102, 1587, 1594
PfoI	TCCNGGA	1 cut(s)	663
PkrI	GCNGC	9 cut(s)	98, 199, 241, 274, 469, 721, 770, 815, 1614
PleI	GAGTC	3 cut(s)	1301, 1418, 1657
PpsI	GAGTC	3 cut(s)	1301, 1418, 1657
Ppu21I	YACGTR	1 cut(s)	1357
PscI	ACATGT	1 cut(s)	1119
Psp6I	CCWGG	5 cut(s)	186, 663, 1038, 1188, 1887
PspFI	CCCAGC	2 cut(s)	715, 1067
PspGI	CCWGG	5 cut(s)	186, 663, 1038, 1188, 1887
PspN4I	GGNNCC	5 cut(s)	713, 1135, 1264, 1402, 1749
PspPI	GGNCC	4 cut(s)	1133, 1143, 1262, 1748
PstI	CTGCAG	3 cut(s)	724, 1328, 1553
PvuII	CAGCTG	1 cut(s)	719
RsaI	GTAC	6 cut(s)	1025, 1118, 1355, 1402, 1628, 1878
RsaNI	GTAC	6 cut(s)	1024, 1117, 1354, 1401, 1627, 1877
RseI	CAYNNNNRTG	2 cut(s)	47, 1211
SapI	GCTCTTC	1 cut(s)	1824
SaqAI	TTAA	3 cut(s)	450, 519, 1895
SatI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 814, 1613
Sau3AI	GATC	3 cut(s)	445, 1107, 1149
Sau96I	GGNCC	4 cut(s)	1133, 1143, 1262, 1748
SchI	GAGTC	3 cut(s)	1302, 1418, 1658
ScrFI	CCNGG	8 cut(s)	188, 665, 1040, 1138, 1190, 1752, 1833, 1889
SfaNI	GCATC	3 cut(s)	1009, 1188, 1282
SfcI	CTRYAG	3 cut(s)	720, 1324, 1549
Sfr274I	CTCGAG	1 cut(s)	1630
SinI	GGWCC	3 cut(s)	1133, 1143, 1748
SlaI	CTCGAG	1 cut(s)	1630
SmiMI	CAYNNNNRTG	2 cut(s)	47, 1211
SmlI	CTYRAG	3 cut(s)	449, 518, 1630
SmoI	CTYRAG	3 cut(s)	449, 518, 1630
Sse9I	AATT	8 cut(s)	33, 579, 611, 1002, 1373, 1655, 1823, 1837
SsiI	CCGC	3 cut(s)	272, 1519, 1612
SspI	AATATT	1 cut(s)	389
SspMI	CTAG	4 cut(s)	605, 1688, 1712, 1903
StyD4I	CCNGG	8 cut(s)	186, 663, 1038, 1136, 1188, 1750, 1831, 1887
StyI	CCWWGG	2 cut(s)	129, 604
TaaI	ACNGT	7 cut(s)	638, 1093, 1388, 1405, 1435, 1639, 1722
TaiI	ACGT	1 cut(s)	1359
TaqI	TCGA	5 cut(s)	119, 258, 342, 375, 1631
TasI	AATT	8 cut(s)	33, 579, 611, 1002, 1373, 1655, 1823, 1837
TatI	WGTACW	2 cut(s)	1116, 1626
TauI	GCSGC	2 cut(s)	275, 1615
TfiI	GAWTC	8 cut(s)	205, 365, 485, 502, 1035, 1102, 1587, 1594
Tru1I	TTAA	3 cut(s)	450, 519, 1895
Tru9I	TTAA	3 cut(s)	450, 519, 1895
TscAI	CASTG	6 cut(s)	331, 469, 1201, 1287, 1534, 1725
TseFI	GTSAC	2 cut(s)	1421, 1492
TseI	GCWGC	7 cut(s)	96, 197, 239, 467, 719, 768, 813
Tsp45I	GTSAC	2 cut(s)	1421, 1492
TspDTI	ATGAA	7 cut(s)	443, 545, 641, 702, 1195, 1216, 1402
TspGWI	ACGGA	2 cut(s)	698, 1817
TspRI	CASTG	6 cut(s)	331, 469, 1201, 1287, 1534, 1725
Vha464I	CTTAAG	2 cut(s)	449, 518
VpaK11BI	GGWCC	3 cut(s)	1133, 1143, 1748
XapI	RAATTY	3 cut(s)	33, 611, 1655
XceI	RCATGY	1 cut(s)	1123
XcmI	CCANNNNNNNNNTGG	2 cut(s)	331, 967
XhoI	CTCGAG	1 cut(s)	1630
XmaJI	CCTAGG	1 cut(s)	604
XmnI	GAANNNNTTC	3 cut(s)	381, 458, 734
XspI	CTAG	4 cut(s)	605, 1688, 1712, 1903
Zsp2I	ATGCAT	2 cut(s)	179, 494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.