Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0003768

Physical Location & Seq

Genomic Coordinates Forward (+)

24564286 .. 24566538

Gene Structure 2253 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g21500.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGGAAAGGAAGGGAAAGGCAGTAGTAGGAGGGATTTCAATTATCCTTGTCATTGGAGCGGTGGTTGGCTGTGTAGTTGTTGTTTCTACTCGCAACAAAGGCAAGGACAAGCTCTCGTCGAGTTCCAAGGCCGTCATTGCAATGTGTGAACCAACTGATTACAAAGAAGCATGCGTTGATAGCCTGGCTGGCGCTGCCAAGAATCAAAGCGCCAGCCCGAAAGACCTCATCCGATCCGCCGTTGAATACACAGTCCAGCAGGTGAAAGCGGCAATAGAGAAATCAGGGATCATTATCAAAGAAGCTAAAGATGTGAATTCCACTGGGAAAATGGCCTTCGATGATTGCAACGAATTGTTGGAATCAGCAATCGAGAGTCTTCAGTATTCGCTTCTGACGCTGGTGGATGCTGATATGCCCAAAATCAATGAGAAAGAGGCTGATCTTAAGAACTGGTTCAGTGCTGCGATGTCATTTCAGGAATCATGCATTGATGGGATTCCTTACGAAGACCTTAAGAAACAAATGAGCGAGGCGATGCAGAATGCTGAACAACTGACCAGCAACGCCTTAGCAATTGTGTCCTCCCTTTCTAATATCCTAGGCAACTTCAACATCCCTTTGAAACCTAACAATAACCCTTCAACCGCTCGTCGTCTCCTGGATGTGGAAATGGAAACAGATGAACATGGCACTTTTCCGACATGGTTTTCGGCTGCAGACAGAAAGCTTATAGCCAAACAGAACAAGAAAAACAACAACGCCGGCAACGGACAAGCTGTTAAACCCAATGCAGTAGTGGCAAAAGATGGGAGTGGACAATACAAAACAATTGGTGCAGCTCTTGCTGCATACCCAAAAGGATTTCAGGGAAGATATGTTATATATGTGAAGGCTGGGATTTATAATGAGTATGTCACTGTGGCCAAGAATCAGAACAATGTGTTCATGTATGGAGATGGACCCCGGGAGACAATGGTCACCGGCCGTAAAAGCTTTGCTGATGGCATCACCACCCAGGACACTGCCACATTCATCGTAGTGGGACACGGGTTCATAGCGAGAGGAATGGGATTTCAGAACACAGCCGGCCCCGAAAAGCATCAGGCAGTGGCGCTCCGAGTCCAGTCGGATATGTCAGCCTTCTTCAACTGCAGAATGGACGGCTACCAAGACACATTGTACGTGCAAGCCTATCGCCAATTTTATCGCAACTGTGTCATATCCGGCACCGTTGACTTCATTTTTGGTGACTCAACAACAGTCATCCAGAACAGTTTGATCATAGTGAGGAAGCCTCTGGCTAACCAAAAGAACACGGTGACTGCTAATGGAAGAAAAGACCAGCGGGAAAGCACTGGTCTAGTTATCCAGAACTGCAGGATAGTCCCGGAAGAGGCTTTGTTCCCATTGAGATTCCAGATTATGTCATACTTGGGCCGGCCATGGAAGATGTACTCGAGAACAGTTATCATGGAATCCGAATTTGGGGACTTTATCCAACCAGCAGGTTGGCTAGAATGGCAAGGAAATTTTGCACTAGATACACTGTATTATGCAGAGTATGCAAATCGGGGTCCGGGTGCTGTAACCACAGGAAGGGTAAAATGGAAAGGATTTCATGTGATTACGGACAGGAATGTGGCTCTTCAATTCACTCCTGGTAACTTCCTAGCAGGAGGCCTTTGGTTGAAAGGCACCGGTGCACCCTACTTCCTGGGTTTAAAAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

578

Amino Acids

63.32

Weight (kDa)

8.82

Isoelectric Point (pI)

26.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	45 - 193	1.3e-29	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	264 - 560	1.6e-136	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003768)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47030 AT2G47040 AT2G47050 AT3G62170 AT3G62180 AT3G62180
fragaria_vesca	FvH4_1g26360 FvH4_4g21500
malus_domestica	MD16G1136400.v1.1 MD17G1283800.v1.1
prunus_persica	Prupe.1G105400_v2.0.a1 Prupe.3G003700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0486131 RchiOBHm_Chr4g0427511
rosa_laevigata	RLG00000007232 RLG00000023101
rosa_multiflora	Rmu_sc0001006.1_g000002 Rmu_sc0009627.1_g000001 Rmu_sc0017895.1_g000001 Rmu_sc0017895.1_g000002
rosa_roxburghii	Rroxscaffold_5G00369150 Rroxscaffold_6G00396030
rosa_rugosa	Rorug03G0223700 Rorug04G0218200
rosa_samantha	Rh3AG273500 Rh3BG308600 Rh3CG307100 Rh3DG303400 Rh4AG274700 Rh4BG280500 Rh4CG295800 Rh4DG277900
rosa_wichuraiana	Rw3G024240 Rw4G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	906
AarI	CACCTGC	1 cut(s)	250
Acc36I	ACCTGC	2 cut(s)	250, 1499
AccB1I	GGYRCC	2 cut(s)	1229, 1697
AccBSI	CCGCTC	2 cut(s)	59, 650
AciI	CCGC	5 cut(s)	59, 237, 269, 648, 1348
AclWI	GGATC	2 cut(s)	228, 296
AcoI	YGGCCR	3 cut(s)	924, 985, 1442
AcsI	RAATTY	3 cut(s)	316, 1484, 1531
AcuI	CTGAAG	1 cut(s)	365
AfaI	GTAC	2 cut(s)	1184, 1457
AfiI	CCNNNNNNNGG	4 cut(s)	667, 1396, 1488, 1599
AflII	CTTAAG	2 cut(s)	446, 515
AgeI	ACCGGT	1 cut(s)	1700
AgsI	TTSAA	8 cut(s)	39, 245, 613, 625, 645, 1150, 1652, 1693
AjnI	CCWGG	5 cut(s)	183, 660, 1017, 1660, 1716
AjuI	GAANNNNNNNTTGG	2 cut(s)	1470, 1502
AluBI	AGCT	6 cut(s)	112, 305, 730, 779, 842, 996
AluI	AGCT	6 cut(s)	112, 305, 730, 779, 842, 996
Alw21I	GWGCWC	1 cut(s)	1708
Alw26I	GTCTC	2 cut(s)	662, 965
Alw44I	GTGCAC	1 cut(s)	1704
AlwI	GGATC	2 cut(s)	228, 296
Ama87I	CYCGRG	2 cut(s)	966, 1459
AoxI	GGCC	8 cut(s)	129, 333, 924, 985, 1090, 1438, 1442, 1681
ApaLI	GTGCAC	1 cut(s)	1704
ApeKI	GCWGC	5 cut(s)	194, 464, 716, 839, 848
ApoI	RAATTY	3 cut(s)	316, 1484, 1531
ArsI	GACNNNNNNTTYG	4 cut(s)	694, 726, 1229, 1261
AsiGI	ACCGGT	1 cut(s)	1700
Asp700I	GAANNNNTTC	1 cut(s)	455
AspA2I	CCTAGG	1 cut(s)	601
AspLEI	GCGC	3 cut(s)	194, 212, 1117
AspS9I	GGNCC	4 cut(s)	962, 1091, 1438, 1577
AsuC2I	CCSGG	4 cut(s)	967, 968, 1391, 1581
AsuHPI	GGTGA	5 cut(s)	274, 973, 1003, 1262, 1333
AvaI	CYCGRG	2 cut(s)	966, 1459
AvaII	GGWCC	2 cut(s)	962, 1577
AvrII	CCTAGG	1 cut(s)	601
BaeGI	GKGCMC	1 cut(s)	1708
BalI	TGGCCA	1 cut(s)	926
BanI	GGYRCC	2 cut(s)	1229, 1697
BbsI	GAAGAC	2 cut(s)	371, 516
Bbv12I	GWGCWC	1 cut(s)	1708
BbvI	GCAGC	5 cut(s)	181, 451, 703, 835, 851
BccI	CCATC	4 cut(s)	488, 803, 953, 998
BceAI	ACGGC	4 cut(s)	116, 224, 972, 1180
BcgI	CGANNNNNNTGC	2 cut(s)	1178, 1212
BciT130I	CCWGG	5 cut(s)	185, 662, 1019, 1662, 1718
BclI	TGATCA	1 cut(s)	1281
BcnI	CCSGG	4 cut(s)	967, 968, 1391, 1581
BcoDI	GTCTC	2 cut(s)	662, 965
BfaI	CTAG	5 cut(s)	602, 1364, 1517, 1541, 1673
BfmI	CTRYAG	3 cut(s)	717, 1153, 1378
BfoI	RGCGCY	3 cut(s)	195, 213, 1118
BfrI	CTTAAG	2 cut(s)	446, 515
BfuAI	ACCTGC	2 cut(s)	250, 1499
BglI	GCCNNNNNGGC	1 cut(s)	189
BisI	GCNGC	6 cut(s)	195, 270, 465, 717, 840, 849
BlnI	CCTAGG	1 cut(s)	601
BlsI	GCNGC	6 cut(s)	196, 271, 466, 718, 841, 850
Bme1390I	CCNGG	9 cut(s)	185, 662, 967, 968, 1019, 1391, 1581, 1662, 1718
Bme18I	GGWCC	2 cut(s)	962, 1577
BmeT110I	CYCGRG	2 cut(s)	966, 1459
BmgT120I	GGNCC	4 cut(s)	962, 1091, 1438, 1577
BmiI	GGNNCC	5 cut(s)	964, 1093, 1231, 1578, 1699
BmrFI	CCNGG	9 cut(s)	185, 662, 967, 968, 1019, 1391, 1581, 1662, 1718
BmrI	ACTGGG	1 cut(s)	333
BmsI	GCATC	4 cut(s)	397, 528, 1017, 1111
BmuI	ACTGGG	1 cut(s)	333
BoxI	GACNNNNGTC	1 cut(s)	977
BpiI	GAAGAC	2 cut(s)	371, 516
BplI	GAGNNNNNCTC	2 cut(s)	1282, 1314
Bpu10I	CCTNAGC	1 cut(s)	571
BpuMI	CCSGG	4 cut(s)	967, 968, 1391, 1581
BsaAI	YACGTR	1 cut(s)	1186
BsaBI	GATNNNNATC	1 cut(s)	293
BsaJI	CCNNGG	7 cut(s)	126, 601, 965, 966, 1017, 1445, 1717
BsaWI	WCCGGW	1 cut(s)	1700
BsaXI	ACNNNNNCTCC	2 cut(s)	48, 78
Bsc4I	CCNNNNNNNGG	4 cut(s)	667, 1396, 1488, 1599
Bse118I	RCCGGY	5 cut(s)	764, 983, 1088, 1440, 1700
Bse1I	ACTGG	4 cut(s)	328, 458, 1126, 1363
Bse3DI	GCAATG	2 cut(s)	135, 147
Bse8I	GATNNNNATC	1 cut(s)	293
BseBI	CCWGG	5 cut(s)	185, 662, 1019, 1662, 1718
BseDI	CCNNGG	7 cut(s)	126, 601, 965, 966, 1017, 1445, 1717
BseGI	GGATG	5 cut(s)	228, 412, 615, 670, 1266
BseJI	GATNNNNATC	1 cut(s)	293
BseLI	CCNNNNNNNGG	4 cut(s)	667, 1396, 1488, 1599
BseMI	GCAATG	2 cut(s)	135, 147
BseNI	ACTGG	4 cut(s)	328, 458, 1126, 1363
BseSI	GKGCMC	1 cut(s)	1708
BseX3I	CGGCCG	1 cut(s)	985
BseXI	GCAGC	5 cut(s)	181, 451, 703, 835, 851
BseYI	CCCAGC	1 cut(s)	896
BsgI	GTGCAG	1 cut(s)	858
Bsh1285I	CGRYCG	1 cut(s)	988
BshFI	GGCC	8 cut(s)	131, 335, 926, 987, 1092, 1440, 1444, 1683
BshNI	GGYRCC	2 cut(s)	1229, 1697
BshTI	ACCGGT	1 cut(s)	1700
BsiEI	CGRYCG	1 cut(s)	988
BsiHKAI	GWGCWC	1 cut(s)	1708
BsiHKCI	CYCGRG	2 cut(s)	966, 1459
BsiSI	CCGG	9 cut(s)	765, 967, 984, 1089, 1227, 1391, 1441, 1580, 1701
BslFI	GGGAC	3 cut(s)	1059, 1373, 1505
BslI	CCNNNNNNNGG	4 cut(s)	667, 1396, 1488, 1599
BsmAI	GTCTC	2 cut(s)	662, 965
BsmBI	CGTCTC	1 cut(s)	662
BsmFI	GGGAC	3 cut(s)	1059, 1373, 1505
BsmI	GAATGC	1 cut(s)	550
BsnI	GGCC	8 cut(s)	131, 335, 926, 987, 1092, 1440, 1444, 1683
BsoBI	CYCGRG	2 cut(s)	966, 1459
Bsp1286I	GDGCHC	1 cut(s)	1708
Bsp143I	GATC	4 cut(s)	233, 288, 442, 1281
Bsp19I	CCATGG	1 cut(s)	1445
BspACI	CCGC	5 cut(s)	59, 237, 269, 648, 1348
BspANI	GGCC	8 cut(s)	131, 335, 926, 987, 1092, 1440, 1444, 1683
BspLI	GGNNCC	5 cut(s)	964, 1093, 1231, 1578, 1699
BspMAI	CTGCAG	3 cut(s)	721, 1157, 1382
BspMI	ACCTGC	2 cut(s)	250, 1499
BspPI	GGATC	2 cut(s)	228, 296
BspQI	GCTCTTC	1 cut(s)	1653
BspT107I	GGYRCC	2 cut(s)	1229, 1697
BspTI	CTTAAG	2 cut(s)	446, 515
BsrBI	CCGCTC	2 cut(s)	59, 650
BsrDI	GCAATG	2 cut(s)	135, 147
BsrFI	RCCGGY	5 cut(s)	764, 983, 1088, 1440, 1700
BsrI	ACTGG	4 cut(s)	328, 458, 1126, 1363
BssAI	RCCGGY	5 cut(s)	764, 983, 1088, 1440, 1700
BssECI	CCNNGG	7 cut(s)	126, 601, 965, 966, 1017, 1445, 1717
BssMI	GATC	4 cut(s)	233, 288, 442, 1281
BssT1I	CCWWGG	3 cut(s)	126, 601, 1445
Bst2UI	CCWGG	5 cut(s)	185, 662, 1019, 1662, 1718
Bst4CI	ACNGT	9 cut(s)	253, 922, 1217, 1234, 1264, 1277, 1321, 1468, 1551
Bst6I	CTCTTC	2 cut(s)	1389, 1653
BstAFI	CTTAAG	2 cut(s)	446, 515
BstAPI	GCANNNNNTGC	2 cut(s)	845, 1565
BstBAI	YACGTR	1 cut(s)	1186
BstC8I	GCNNGC	7 cut(s)	172, 190, 214, 766, 1090, 1191, 1442
BstDEI	CTNAG	1 cut(s)	571
BstDSI	CCRYGG	1 cut(s)	1445
BstEII	GGTNACC	1 cut(s)	979
BstF5I	GGATG	5 cut(s)	228, 412, 615, 670, 1266
BstH2I	RGCGCY	3 cut(s)	195, 213, 1118
BstHHI	GCGC	3 cut(s)	194, 212, 1117
BstKTI	GATC	4 cut(s)	236, 291, 445, 1284
BstMAI	GTCTC	2 cut(s)	662, 965
BstMBI	GATC	4 cut(s)	233, 288, 442, 1281
BstMCI	CGRYCG	1 cut(s)	988
BstNI	CCWGG	5 cut(s)	185, 662, 1019, 1662, 1718
BstNSI	RCATGY	1 cut(s)	174
BstPAI	GACNNNNGTC	1 cut(s)	977
BstPI	GGTNACC	1 cut(s)	979
BstSCI	CCNGG	9 cut(s)	183, 660, 965, 966, 1017, 1389, 1579, 1660, 1716
BstSFI	CTRYAG	3 cut(s)	717, 1153, 1378
BstSLI	GKGCMC	1 cut(s)	1708
BstV1I	GCAGC	5 cut(s)	181, 451, 703, 835, 851
BstV2I	GAAGAC	2 cut(s)	371, 516
BstXI	CCANNNNNNTGG	1 cut(s)	1512
BstZI	CGGCCG	1 cut(s)	985
BsuRI	GGCC	8 cut(s)	131, 335, 926, 987, 1092, 1440, 1444, 1683
BtgI	CCRYGG	1 cut(s)	1445
BtgZI	GCGATG	2 cut(s)	482, 551
BtsCI	GGATG	5 cut(s)	228, 412, 615, 670, 1266
BtsI	GCAGTG	2 cut(s)	1023, 1116
BtsIMutI	CAGTG	7 cut(s)	321, 466, 918, 1023, 1116, 1356, 1547
BveI	ACCTGC	2 cut(s)	250, 1499
Cac8I	GCNNGC	7 cut(s)	172, 190, 214, 766, 1090, 1191, 1442
CfoI	GCGC	3 cut(s)	194, 212, 1117
Cfr10I	RCCGGY	5 cut(s)	764, 983, 1088, 1440, 1700
Cfr13I	GGNCC	4 cut(s)	962, 1091, 1438, 1577
Cfr9I	CCCGGG	1 cut(s)	966
CseI	GACGC	1 cut(s)	406
Csp6I	GTAC	2 cut(s)	1183, 1456
CspAI	ACCGGT	1 cut(s)	1700
CviAII	CATG	8 cut(s)	171, 486, 689, 705, 949, 1446, 1474, 1622
CviQI	GTAC	2 cut(s)	1183, 1456
DdeI	CTNAG	1 cut(s)	571
DpnI	GATC	4 cut(s)	235, 290, 444, 1283
DpnII	GATC	4 cut(s)	233, 288, 442, 1281
DraI	TTTAAA	1 cut(s)	1725
EaeI	YGGCCR	3 cut(s)	924, 985, 1442
EagI	CGGCCG	1 cut(s)	985
Eam1104I	CTCTTC	2 cut(s)	1389, 1653
EarI	CTCTTC	2 cut(s)	1389, 1653
EciI	GGCGGA	1 cut(s)	226
EclXI	CGGCCG	1 cut(s)	985
Eco130I	CCWWGG	3 cut(s)	126, 601, 1445
Eco147I	AGGCCT	1 cut(s)	1683
Eco47I	GGWCC	2 cut(s)	962, 1577
Eco52I	CGGCCG	1 cut(s)	985
Eco57I	CTGAAG	1 cut(s)	365
Eco88I	CYCGRG	2 cut(s)	966, 1459
Eco91I	GGTNACC	1 cut(s)	979
EcoO65I	GGTNACC	1 cut(s)	979
EcoRI	GAATTC	1 cut(s)	316
EcoRII	CCWGG	5 cut(s)	183, 660, 1017, 1660, 1716
EcoT14I	CCWWGG	3 cut(s)	126, 601, 1445
EcoT22I	ATGCAT	1 cut(s)	491
ErhI	CCWWGG	3 cut(s)	126, 601, 1445
Esp3I	CGTCTC	1 cut(s)	662
FaeI	CATG	8 cut(s)	174, 489, 692, 708, 952, 1449, 1477, 1625
FaqI	GGGAC	3 cut(s)	1059, 1373, 1505
FatI	CATG	8 cut(s)	170, 485, 688, 704, 948, 1445, 1473, 1621
FauI	CCCGC	1 cut(s)	1341
FbaI	TGATCA	1 cut(s)	1281
Fnu4HI	GCNGC	6 cut(s)	195, 270, 465, 717, 840, 849
FokI	GGATG	5 cut(s)	215, 419, 602, 677, 1253
FseI	GGCCGGCC	1 cut(s)	1444
Fsp4HI	GCNGC	6 cut(s)	195, 270, 465, 717, 840, 849
FspBI	CTAG	5 cut(s)	602, 1364, 1517, 1541, 1673
GlaI	GCGC	3 cut(s)	193, 211, 1116
GluI	GCNGC	6 cut(s)	195, 270, 465, 717, 840, 849
GsaI	CCCAGC	1 cut(s)	900
HaeII	RGCGCY	3 cut(s)	195, 213, 1118
HaeIII	GGCC	8 cut(s)	131, 335, 926, 987, 1092, 1440, 1444, 1683
HapII	CCGG	9 cut(s)	765, 967, 984, 1089, 1227, 1391, 1441, 1580, 1701
HgaI	GACGC	1 cut(s)	406
HhaI	GCGC	3 cut(s)	194, 212, 1117
Hin1II	CATG	8 cut(s)	174, 489, 692, 708, 952, 1449, 1477, 1625
Hin6I	GCGC	3 cut(s)	192, 210, 1115
HinP1I	GCGC	3 cut(s)	192, 210, 1115
HincII	GTYRAC	1 cut(s)	1237
HindII	GTYRAC	1 cut(s)	1237
HindIII	AAGCTT	2 cut(s)	728, 994
HpaII	CCGG	9 cut(s)	765, 967, 984, 1089, 1227, 1391, 1441, 1580, 1701
HphI	GGTGA	5 cut(s)	274, 973, 1003, 1262, 1333
Hpy166II	GTNNAC	4 cut(s)	149, 818, 1237, 1706
Hpy188I	TCNGA	8 cut(s)	233, 396, 702, 936, 1080, 1121, 1132, 1483
Hpy188III	TCNNGA	6 cut(s)	373, 479, 1270, 1372, 1420, 1461
Hpy8I	GTNNAC	4 cut(s)	149, 818, 1237, 1706
Hpy99I	CGWCG	2 cut(s)	121, 657
HpyAV	CCTTC	6 cut(s)	4, 346, 651, 886, 1153, 1593
HpyCH4III	ACNGT	9 cut(s)	253, 922, 1217, 1234, 1264, 1277, 1321, 1468, 1551
HpyCH4IV	ACGT	1 cut(s)	1185
HpyF3I	CTNAG	1 cut(s)	571
HpySE526I	ACGT	1 cut(s)	1185
Hsp92II	CATG	8 cut(s)	174, 489, 692, 708, 952, 1449, 1477, 1625
HspAI	GCGC	3 cut(s)	192, 210, 1115
KroI	GCCGGC	3 cut(s)	764, 1088, 1440
KroNI	GCCGGC	3 cut(s)	766, 1090, 1442
Ksp22I	TGATCA	1 cut(s)	1281
Kzo9I	GATC	4 cut(s)	233, 288, 442, 1281
LguI	GCTCTTC	1 cut(s)	1653
LmnI	GCTCC	2 cut(s)	56, 1122
Lsp1109I	GCAGC	5 cut(s)	181, 451, 703, 835, 851
LweI	GCATC	4 cut(s)	397, 528, 1017, 1111
MaeI	CTAG	5 cut(s)	602, 1364, 1517, 1541, 1673
MaeII	ACGT	1 cut(s)	1185
MaeIII	GTNAC	6 cut(s)	916, 979, 1250, 1321, 1588, 1664
MalI	GATC	4 cut(s)	235, 290, 444, 1283
MbiI	CCGCTC	2 cut(s)	59, 650
MboI	GATC	4 cut(s)	233, 288, 442, 1281
MboII	GAAGA	8 cut(s)	371, 521, 885, 1138, 1347, 1406, 1462, 1640
MfeI	CAATTG	2 cut(s)	576, 831
MhlI	GDGCHC	1 cut(s)	1708
MlsI	TGGCCA	1 cut(s)	926
MluNI	TGGCCA	1 cut(s)	926
MlyI	GAGTC	3 cut(s)	385, 1131, 1247
MmeI	TCCRAC	4 cut(s)	339, 725, 1110, 1525
Mox20I	TGGCCA	1 cut(s)	926
Mph1103I	ATGCAT	1 cut(s)	491
MroNI	GCCGGC	3 cut(s)	764, 1088, 1440
MroXI	GAANNNNTTC	1 cut(s)	455
MscI	TGGCCA	1 cut(s)	926
MseI	TTAA	5 cut(s)	447, 516, 783, 1724, 1732
MslI	CAYNNNNRTG	2 cut(s)	140, 1040
Msp20I	TGGCCA	1 cut(s)	926
MspA1I	CMGCKG	1 cut(s)	1348
MspCI	CTTAAG	2 cut(s)	446, 515
MspI	CCGG	9 cut(s)	765, 967, 984, 1089, 1227, 1391, 1441, 1580, 1701
MspR9I	CCNGG	9 cut(s)	185, 662, 967, 968, 1019, 1391, 1581, 1662, 1718
MunI	CAATTG	2 cut(s)	576, 831
Mva1269I	GAATGC	1 cut(s)	550
MvaI	CCWGG	5 cut(s)	185, 662, 1019, 1662, 1718
NaeI	GCCGGC	3 cut(s)	766, 1090, 1442
NciI	CCSGG	4 cut(s)	967, 968, 1391, 1581
NcoI	CCATGG	1 cut(s)	1445
NdeII	GATC	4 cut(s)	233, 288, 442, 1281
NgoMIV	GCCGGC	3 cut(s)	764, 1088, 1440
NlaIII	CATG	8 cut(s)	174, 489, 692, 708, 952, 1449, 1477, 1625
NlaIV	GGNNCC	5 cut(s)	964, 1093, 1231, 1578, 1699
NmuCI	GTSAC	4 cut(s)	916, 979, 1250, 1321
NsiI	ATGCAT	1 cut(s)	491
NspI	RCATGY	1 cut(s)	174
PaeI	GCATGC	1 cut(s)	174
PaeR7I	CTCGAG	1 cut(s)	1459
PaqCI	CACCTGC	1 cut(s)	250
PceI	AGGCCT	1 cut(s)	1683
PciSI	GCTCTTC	1 cut(s)	1653
PctI	GAATGC	1 cut(s)	550
PdiI	GCCGGC	3 cut(s)	766, 1090, 1442
PdmI	GAANNNNTTC	1 cut(s)	455
PfeI	GAWTC	7 cut(s)	202, 362, 482, 499, 931, 1416, 1478
PfoI	TCCNGGA	2 cut(s)	660, 1389
PinAI	ACCGGT	1 cut(s)	1700
PkrI	GCNGC	6 cut(s)	196, 271, 466, 718, 841, 850
PleI	GAGTC	3 cut(s)	384, 1130, 1247
PpsI	GAGTC	3 cut(s)	384, 1130, 1247
Ppu21I	YACGTR	1 cut(s)	1186
PshAI	GACNNNNGTC	1 cut(s)	977
PsiI	TTATAA	1 cut(s)	906
Psp6I	CCWGG	5 cut(s)	183, 660, 1017, 1660, 1716
PspEI	GGTNACC	1 cut(s)	979
PspFI	CCCAGC	1 cut(s)	896
PspGI	CCWGG	5 cut(s)	183, 660, 1017, 1660, 1716
PspN4I	GGNNCC	5 cut(s)	964, 1093, 1231, 1578, 1699
PspPI	GGNCC	4 cut(s)	962, 1091, 1438, 1577
PstI	CTGCAG	3 cut(s)	721, 1157, 1382
RigI	GGCCGGCC	1 cut(s)	1444
RsaI	GTAC	2 cut(s)	1184, 1457
RsaNI	GTAC	2 cut(s)	1183, 1456
RseI	CAYNNNNRTG	2 cut(s)	140, 1040
SapI	GCTCTTC	1 cut(s)	1653
SaqAI	TTAA	5 cut(s)	447, 516, 783, 1724, 1732
SatI	GCNGC	6 cut(s)	195, 270, 465, 717, 840, 849
Sau3AI	GATC	4 cut(s)	233, 288, 442, 1281
Sau96I	GGNCC	4 cut(s)	962, 1091, 1438, 1577
SchI	GAGTC	3 cut(s)	385, 1131, 1247
ScrFI	CCNGG	9 cut(s)	185, 662, 967, 968, 1019, 1391, 1581, 1662, 1718
SduI	GDGCHC	1 cut(s)	1708
SfaNI	GCATC	4 cut(s)	397, 528, 1017, 1111
SfcI	CTRYAG	3 cut(s)	717, 1153, 1378
Sfr274I	CTCGAG	1 cut(s)	1459
SgrAI	CRCCGGYG	1 cut(s)	1700
SinI	GGWCC	2 cut(s)	962, 1577
SlaI	CTCGAG	1 cut(s)	1459
SmaI	CCCGGG	1 cut(s)	968
SmiMI	CAYNNNNRTG	2 cut(s)	140, 1040
SmlI	CTYRAG	3 cut(s)	446, 515, 1459
SmoI	CTYRAG	3 cut(s)	446, 515, 1459
SphI	GCATGC	1 cut(s)	174
SseBI	AGGCCT	1 cut(s)	1683
SsiI	CCGC	5 cut(s)	59, 237, 269, 648, 1348
SspMI	CTAG	5 cut(s)	602, 1364, 1517, 1541, 1673
StuI	AGGCCT	1 cut(s)	1683
StyD4I	CCNGG	9 cut(s)	183, 660, 965, 966, 1017, 1389, 1579, 1660, 1716
StyI	CCWWGG	3 cut(s)	126, 601, 1445
TaaI	ACNGT	9 cut(s)	253, 922, 1217, 1234, 1264, 1277, 1321, 1468, 1551
TaiI	ACGT	1 cut(s)	1188
TaqI	TCGA	4 cut(s)	119, 339, 372, 1460
TatI	WGTACW	1 cut(s)	1455
TauI	GCSGC	1 cut(s)	272
TfiI	GAWTC	7 cut(s)	202, 362, 482, 499, 931, 1416, 1478
Tru1I	TTAA	5 cut(s)	447, 516, 783, 1724, 1732
Tru9I	TTAA	5 cut(s)	447, 516, 783, 1724, 1732
TscAI	CASTG	7 cut(s)	328, 466, 925, 1030, 1116, 1363, 1554
TseFI	GTSAC	4 cut(s)	916, 979, 1250, 1321
TseI	GCWGC	5 cut(s)	194, 464, 716, 839, 848
Tsp45I	GTSAC	4 cut(s)	916, 979, 1250, 1321
TspDTI	ATGAA	6 cut(s)	699, 937, 1024, 1045, 1231, 1610
TspGWI	ACGGA	2 cut(s)	786, 1646
TspMI	CCCGGG	1 cut(s)	966
TspRI	CASTG	7 cut(s)	328, 466, 925, 1030, 1116, 1363, 1554
Vha464I	CTTAAG	2 cut(s)	446, 515
VneI	GTGCAC	1 cut(s)	1704
VpaK11BI	GGWCC	2 cut(s)	962, 1577
XapI	RAATTY	3 cut(s)	316, 1484, 1531
XceI	RCATGY	1 cut(s)	174
XcmI	CCANNNNNNNNNTGG	2 cut(s)	328, 796
XhoI	CTCGAG	1 cut(s)	1459
XmaI	CCCGGG	1 cut(s)	966
XmaJI	CCTAGG	1 cut(s)	601
XmnI	GAANNNNTTC	1 cut(s)	455
XspI	CTAG	5 cut(s)	602, 1364, 1517, 1541, 1673
Zsp2I	ATGCAT	1 cut(s)	491

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.