Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0003768

Physical Location & Seq

Genomic Coordinates Reverse (-)

18209069 .. 18210816

Gene Structure 1748 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g26360.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1644 bp

ATGGTAGCTAAGAAAGTACTAATATCAAGTATATTTCTCATACTTATTGTGGGGGTGGTCATTGGACTGGTTGCCGTATTCGTTCACAACAATGCGAAGAAGTCCGGACAAGAGAGCCTATCGGAACAAATGAAACTGGTGATCCAAATGTGTAATTACACAGACCACCACACAGAATGTCAGCAATCTCTTAGCCCGGTGGCTCAAAACAGCAGCGCTGGTGTCAAGGACTACATGAAAGCTGCCTTGCTGTCCATCTCCGACCAAGCACAAAACTCCCTCAACATGACAGAGTCTCTCTTGGTTGAGGCCAAAGACGGCACCAGAATCAAGAGGTCCATTGAGGAATGCAATGACCTAATGAAAGAAGCCCGTGACGAGCTACAGGCTTTGGTGTCGTATGTTAATGATGTTGAGTTGGAAACTATGAATGACCGCGCATTGGAGCTCAGAATCTGGATCGGTAGTGTGAATAGCTACGCCGGAACATGCATGGATGTAATTGGAGATGACGACCGTAAGCTAAATGAGAATGCAGTCACCGAAAATGGCAGTAACAGCAGGCGGCTTATGGGTGAAATTCTAGAGGAAGATATCCCTAAAGATGAATACCCAAGCTGGATGTCTCGCGCAGACTGCAAGCTTATGGCCAAGCAAGACAATGGCAGGATTATGCCTAATGTTATTGTAGCTCAAGACGGCAGCGGGCAATTCAGAACCATTAACGAGGCACTGAGGGCTTACCCTAAAGGCAACCAAGGGCGCTTCGTTATTTATGTGAAGCAGGGAGTGTACAAGGAGACTGTCATCATCGACAAAACCATGGTGAACATATACATGTATGGTGATGGCCCCAGAGCCACTGTCGTCACCGGTAACAAGGGAGAGGCGAACACCGGAGATAAAATATCTACCAGCGGCACCTTCCAAGTTTACGCTAGTGGATTCATTGGGAGGTCAATGGGATTCACCAACGGCGCCGGTCCGGAAGCAAGACCCGCCGTGGCTCTCCGCACTTCCGGGGACATGATTGGCATCTTCAACTGTAGAATCAGTGGATACCAAGACACCTTGTACATGAACATTGGACGTCAGTTCTTTAGGAATTGCGTCATTTCAGGACATGTTGACTGTATCTTCGGGGATGCTAGGGTGGTCATCCAAAACTCTTTGATCATCGTGAGGAAGTCGTCGATGAAGAACCAGAACCAAGCCTTCATCACTGCTCCAGGACAACAAACCGGACACGAAGTCGGAGCCACCGTGCTGCACAACTGCAGAATCGTGCCGGAAGAAGCACTCTTCCCCACGAGGTTCGCAGTGAGAACATACTTGGGGAGGCCATGGAAAACCTACGCTAGGGCTGCGTTCATAGAGTGCACAATAGCTGACTTCATCAGACCTGAAGGGGTTATCGCATGGGACGGTAACGACTTCAACACTCAACACTCCTTCTTTGGTGAGTTCAAGAACCGCGGACCTGGTGCCAACATAGCCAACAGGGTGAAATGGCCGAAGGGCGGCTTCACCGGCGAGATGGATGGGAATACTGCTGCGAAATTTACCGTTGATTCCATGCTATTACCAGACACATGGTTGAAGATGGCAACTATTCCTTACTTACCTAGCTTCGCACATGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

548

Amino Acids

60.43

Weight (kDa)

6.73

Isoelectric Point (pI)

36.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	46 - 187	7.2e-15	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	227 - 527	3.5e-99	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003768)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47030 AT2G47040 AT2G47050 AT3G62170 AT3G62180 AT3G62180
fragaria_vesca	FvH4_1g26360 FvH4_4g21500
malus_domestica	MD16G1136400.v1.1 MD17G1283800.v1.1
prunus_persica	Prupe.1G105400_v2.0.a1 Prupe.3G003700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0486131 RchiOBHm_Chr4g0427511
rosa_laevigata	RLG00000007232 RLG00000023101
rosa_multiflora	Rmu_sc0001006.1_g000002 Rmu_sc0009627.1_g000001 Rmu_sc0017895.1_g000001 Rmu_sc0017895.1_g000002
rosa_roxburghii	Rroxscaffold_5G00369150 Rroxscaffold_6G00396030
rosa_rugosa	Rorug03G0223700 Rorug04G0218200
rosa_samantha	Rh3AG273500 Rh3BG308600 Rh3CG307100 Rh3DG303400 Rh4AG274700 Rh4BG280500 Rh4CG295800 Rh4DG277900
rosa_wichuraiana	Rw3G024240 Rw4G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1093
AccB1I	GGYRCC	4 cut(s)	320, 920, 977, 1484
AccII	CGCG	3 cut(s)	438, 630, 1476
AccIII	TCCGGA	2 cut(s)	104, 985
AciI	CCGC	9 cut(s)	436, 565, 705, 918, 999, 1012, 1474, 1476, 1521
AclWI	GGATC	2 cut(s)	136, 467
AcoI	YGGCCR	2 cut(s)	648, 1511
AcsI	RAATTY	2 cut(s)	579, 1559
AcuI	CTGAAG	1 cut(s)	1425
AcyI	GRCGYC	2 cut(s)	978, 1090
AfaI	GTAC	3 cut(s)	18, 794, 1076
AfeI	AGCGCT	1 cut(s)	217
AfiI	CCNNNNNNNGG	3 cut(s)	442, 1359, 1520
AflIII	ACRYGT	2 cut(s)	837, 1123
AgeI	ACCGGT	1 cut(s)	872
AgsI	TTSAA	4 cut(s)	1042, 1438, 1468, 1600
AhdI	GACNNNNNGTC	1 cut(s)	1250
AjnI	CCWGG	2 cut(s)	1228, 1480
Alw21I	GWGCWC	2 cut(s)	450, 1382
Alw26I	GTCTC	3 cut(s)	300, 630, 794
Alw44I	GTGCAC	1 cut(s)	1378
AlwI	GGATC	2 cut(s)	136, 467
AlwNI	CAGNNNCTG	1 cut(s)	456
Aor13HI	TCCGGA	2 cut(s)	104, 985
Aor51HI	AGCGCT	1 cut(s)	217
AoxI	GGCC	5 cut(s)	309, 648, 850, 1340, 1511
ApaLI	GTGCAC	1 cut(s)	1378
ApeKI	GCWGC	6 cut(s)	213, 242, 702, 1267, 1364, 1553
ApoI	RAATTY	2 cut(s)	579, 1559
ArsI	GACNNNNNNTTYG	2 cut(s)	1121, 1153
AsiGI	ACCGGT	1 cut(s)	872
AspLEI	GCGC	5 cut(s)	218, 440, 632, 765, 980
AspS9I	GGNCC	4 cut(s)	336, 851, 983, 1478
AsuC2I	CCSGG	2 cut(s)	197, 1021
AvaII	GGWCC	3 cut(s)	336, 983, 1478
BaeGI	GKGCMC	1 cut(s)	1382
BalI	TGGCCA	1 cut(s)	650
BanI	GGYRCC	4 cut(s)	320, 920, 977, 1484
BanII	GRGCYC	1 cut(s)	450
BauI	CACGAG	1 cut(s)	1309
Bbv12I	GWGCWC	2 cut(s)	450, 1382
BbvI	GCAGC	6 cut(s)	225, 229, 714, 1254, 1351, 1540
BccI	CCATC	5 cut(s)	263, 842, 1531, 1535, 1597
BceAI	ACGGC	5 cut(s)	59, 334, 715, 986, 991
BciT130I	CCWGG	2 cut(s)	1230, 1482
BciVI	GTATCC	1 cut(s)	1052
BclI	TGATCA	1 cut(s)	1173
BcnI	CCSGG	2 cut(s)	197, 1021
BcoDI	GTCTC	3 cut(s)	300, 630, 794
BfaI	CTAG	5 cut(s)	584, 939, 1149, 1359, 1626
BfmI	CTRYAG	3 cut(s)	383, 1045, 1276
BfoI	RGCGCY	3 cut(s)	219, 766, 981
BfuI	GTATCC	1 cut(s)	1052
BisI	GCNGC	9 cut(s)	214, 243, 566, 703, 919, 1268, 1365, 1522, 1554
BlsI	GCNGC	9 cut(s)	215, 244, 567, 704, 920, 1269, 1366, 1523, 1555
BmcAI	AGTACT	1 cut(s)	18
Bme1390I	CCNGG	4 cut(s)	197, 1021, 1230, 1482
Bme18I	GGWCC	3 cut(s)	336, 983, 1478
BmeRI	GACNNNNNGTC	1 cut(s)	1250
BmgT120I	GGNCC	4 cut(s)	336, 851, 983, 1478
BmiI	GGNNCC	6 cut(s)	322, 853, 922, 979, 1258, 1486
BmrFI	CCNGG	4 cut(s)	197, 1021, 1230, 1482
BmsI	GCATC	2 cut(s)	1044, 1135
BpmI	CTGGAG	1 cut(s)	1212
BpuEI	CTTGAG	1 cut(s)	678
BpuMI	CCSGG	2 cut(s)	197, 1021
BsaBI	GATNNNNATC	1 cut(s)	1034
BsaHI	GRCGYC	2 cut(s)	978, 1090
BsaJI	CCNNGG	6 cut(s)	757, 822, 1002, 1020, 1343, 1474
BsaWI	WCCGGW	5 cut(s)	104, 872, 896, 985, 1241
BsaXI	ACNNNNNCTCC	2 cut(s)	1248, 1278
Bsc4I	CCNNNNNNNGG	3 cut(s)	442, 1359, 1520
Bse118I	RCCGGY	3 cut(s)	872, 980, 1529
Bse1I	ACTGG	2 cut(s)	72, 141
Bse3DI	GCAATG	1 cut(s)	358
Bse8I	GATNNNNATC	1 cut(s)	1034
BseAI	TCCGGA	2 cut(s)	104, 985
BseBI	CCWGG	2 cut(s)	1230, 1482
BseDI	CCNNGG	6 cut(s)	757, 822, 1002, 1020, 1343, 1474
BseGI	GGATG	5 cut(s)	502, 627, 1150, 1158, 1546
BseJI	GATNNNNATC	1 cut(s)	1034
BseLI	CCNNNNNNNGG	3 cut(s)	442, 1359, 1520
BseMI	GCAATG	1 cut(s)	358
BseMII	CTCAG	2 cut(s)	463, 725
BseNI	ACTGG	2 cut(s)	72, 141
BseSI	GKGCMC	1 cut(s)	1382
BseXI	GCAGC	6 cut(s)	225, 229, 714, 1254, 1351, 1540
BsgI	GTGCAG	1 cut(s)	1253
Bsh1236I	CGCG	3 cut(s)	438, 630, 1476
Bsh1285I	CGRYCG	1 cut(s)	517
BshFI	GGCC	5 cut(s)	311, 650, 852, 1342, 1513
BshNI	GGYRCC	4 cut(s)	320, 920, 977, 1484
BshTI	ACCGGT	1 cut(s)	872
BsiEI	CGRYCG	1 cut(s)	517
BsiHKAI	GWGCWC	2 cut(s)	450, 1382
BslFI	GGGAC	2 cut(s)	1037, 1436
BslI	CCNNNNNNNGG	3 cut(s)	442, 1359, 1520
BsmAI	GTCTC	3 cut(s)	300, 630, 794
BsmFI	GGGAC	2 cut(s)	1037, 1436
BsmI	GAATGC	2 cut(s)	353, 538
BsnI	GGCC	5 cut(s)	311, 650, 852, 1342, 1513
Bsp1286I	GDGCHC	2 cut(s)	450, 1382
Bsp13I	TCCGGA	2 cut(s)	104, 985
Bsp1407I	TGTACA	2 cut(s)	792, 1074
Bsp143I	GATC	3 cut(s)	141, 459, 1173
Bsp19I	CCATGG	2 cut(s)	822, 1343
BspACI	CCGC	9 cut(s)	436, 565, 705, 918, 999, 1012, 1474, 1476, 1521
BspANI	GGCC	5 cut(s)	311, 650, 852, 1342, 1513
BspCNI	CTCAG	2 cut(s)	462, 726
BspEI	TCCGGA	2 cut(s)	104, 985
BspFNI	CGCG	3 cut(s)	438, 630, 1476
BspLI	GGNNCC	6 cut(s)	322, 853, 922, 979, 1258, 1486
BspMAI	CTGCAG	1 cut(s)	1280
BspPI	GGATC	2 cut(s)	136, 467
BspT107I	GGYRCC	4 cut(s)	320, 920, 977, 1484
BsrDI	GCAATG	1 cut(s)	358
BsrFI	RCCGGY	3 cut(s)	872, 980, 1529
BsrGI	TGTACA	2 cut(s)	792, 1074
BsrI	ACTGG	2 cut(s)	72, 141
BssAI	RCCGGY	3 cut(s)	872, 980, 1529
BssECI	CCNNGG	6 cut(s)	757, 822, 1002, 1020, 1343, 1474
BssMI	GATC	3 cut(s)	141, 459, 1173
BssNI	GRCGYC	2 cut(s)	978, 1090
BssSI	CACGAG	1 cut(s)	1309
BssT1I	CCWWGG	3 cut(s)	757, 822, 1343
Bst2BI	CACGAG	1 cut(s)	1309
Bst2UI	CCWGG	2 cut(s)	1230, 1482
Bst4CI	ACNGT	8 cut(s)	518, 805, 865, 1046, 1133, 1264, 1427, 1567
Bst6I	CTCTTC	1 cut(s)	1307
BstACI	GRCGYC	2 cut(s)	978, 1090
BstAUI	TGTACA	2 cut(s)	792, 1074
BstC8I	GCNNGC	3 cut(s)	563, 641, 707
BstDEI	CTNAG	4 cut(s)	9, 191, 449, 734
BstDSI	CCRYGG	4 cut(s)	822, 1002, 1343, 1474
BstENI	CCTNNNNNAGG	1 cut(s)	1357
BstF5I	GGATG	5 cut(s)	502, 627, 1150, 1158, 1546
BstFNI	CGCG	3 cut(s)	438, 630, 1476
BstH2I	RGCGCY	3 cut(s)	219, 766, 981
BstHHI	GCGC	5 cut(s)	218, 440, 632, 765, 980
BstKTI	GATC	3 cut(s)	144, 462, 1176
BstMAI	GTCTC	3 cut(s)	300, 630, 794
BstMBI	GATC	3 cut(s)	141, 459, 1173
BstMCI	CGRYCG	1 cut(s)	517
BstMWI	GCNNNNNNNGC	7 cut(s)	558, 636, 737, 998, 1364, 1493, 1530
BstNI	CCWGG	2 cut(s)	1230, 1482
BstNSI	RCATGY	4 cut(s)	492, 841, 1127, 1640
BstSCI	CCNGG	4 cut(s)	195, 1019, 1228, 1480
BstSFI	CTRYAG	3 cut(s)	383, 1045, 1276
BstSLI	GKGCMC	1 cut(s)	1382
BstUI	CGCG	3 cut(s)	438, 630, 1476
BstV1I	GCAGC	6 cut(s)	225, 229, 714, 1254, 1351, 1540
BstXI	CCANNNNNNTGG	1 cut(s)	1593
BsuI	GTATCC	1 cut(s)	1052
BsuRI	GGCC	5 cut(s)	311, 650, 852, 1342, 1513
BtgI	CCRYGG	4 cut(s)	822, 1002, 1343, 1474
BtsCI	GGATG	5 cut(s)	502, 627, 1150, 1158, 1546
BtsI	GCAGTG	2 cut(s)	1221, 1326
BtsIMutI	CAGTG	5 cut(s)	731, 861, 1060, 1221, 1326
Cac8I	GCNNGC	3 cut(s)	563, 641, 707
CaiI	CAGNNNCTG	1 cut(s)	456
CfoI	GCGC	5 cut(s)	218, 440, 632, 765, 980
Cfr10I	RCCGGY	3 cut(s)	872, 980, 1529
Cfr13I	GGNCC	4 cut(s)	336, 851, 983, 1478
Cfr42I	CCGCGG	1 cut(s)	1477
CpoI	CGGWCCG	1 cut(s)	983
CseI	GACGC	1 cut(s)	1099
CsiI	ACCWGGT	1 cut(s)	1480
Csp6I	GTAC	3 cut(s)	17, 793, 1075
CspAI	ACCGGT	1 cut(s)	872
CspI	CGGWCCG	1 cut(s)	983
CviQI	GTAC	3 cut(s)	17, 793, 1075
DdeI	CTNAG	4 cut(s)	9, 191, 449, 734
DinI	GGCGCC	1 cut(s)	979
DpnI	GATC	3 cut(s)	143, 461, 1175
DpnII	GATC	3 cut(s)	141, 459, 1173
DriI	GACNNNNNGTC	1 cut(s)	1250
EaeI	YGGCCR	2 cut(s)	648, 1511
Eam1104I	CTCTTC	1 cut(s)	1307
Eam1105I	GACNNNNNGTC	1 cut(s)	1250
EarI	CTCTTC	1 cut(s)	1307
Ecl136II	GAGCTC	1 cut(s)	448
Eco130I	CCWWGG	3 cut(s)	757, 822, 1343
Eco24I	GRGCYC	1 cut(s)	450
Eco32I	GATATC	1 cut(s)	595
Eco47I	GGWCC	3 cut(s)	336, 983, 1478
Eco47III	AGCGCT	1 cut(s)	217
Eco53kI	GAGCTC	1 cut(s)	448
Eco57I	CTGAAG	1 cut(s)	1425
EcoICRI	GAGCTC	1 cut(s)	448
EcoNI	CCTNNNNNAGG	1 cut(s)	1357
EcoRII	CCWGG	2 cut(s)	1228, 1480
EcoRV	GATATC	1 cut(s)	595
EcoT14I	CCWWGG	3 cut(s)	757, 822, 1343
EcoT22I	ATGCAT	1 cut(s)	494
EcoT38I	GRGCYC	1 cut(s)	450
EgeI	GGCGCC	1 cut(s)	979
EheI	GGCGCC	1 cut(s)	979
ErhI	CCWWGG	3 cut(s)	757, 822, 1343
FalI	AAGNNNNNCTT	2 cut(s)	1508, 1540
FaqI	GGGAC	2 cut(s)	1037, 1436
FauI	CCCGC	2 cut(s)	698, 1006
FbaI	TGATCA	1 cut(s)	1173
Fnu4HI	GCNGC	9 cut(s)	214, 243, 566, 703, 919, 1268, 1365, 1522, 1554
FokI	GGATG	5 cut(s)	509, 634, 1145, 1157, 1553
FriOI	GRGCYC	1 cut(s)	450
Fsp4HI	GCNGC	9 cut(s)	214, 243, 566, 703, 919, 1268, 1365, 1522, 1554
FspBI	CTAG	5 cut(s)	584, 939, 1149, 1359, 1626
GlaI	GCGC	5 cut(s)	217, 439, 631, 764, 979
GluI	GCNGC	9 cut(s)	214, 243, 566, 703, 919, 1268, 1365, 1522, 1554
GsuI	CTGGAG	1 cut(s)	1212
HaeII	RGCGCY	3 cut(s)	219, 766, 981
HaeIII	GGCC	5 cut(s)	311, 650, 852, 1342, 1513
HgaI	GACGC	1 cut(s)	1099
HhaI	GCGC	5 cut(s)	218, 440, 632, 765, 980
Hin1I	GRCGYC	2 cut(s)	978, 1090
Hin6I	GCGC	5 cut(s)	216, 438, 630, 763, 978
HinP1I	GCGC	5 cut(s)	216, 438, 630, 763, 978
HincII	GTYRAC	1 cut(s)	1129
HindII	GTYRAC	1 cut(s)	1129
HindIII	AAGCTT	1 cut(s)	641
HinfI	GANTC	8 cut(s)	293, 327, 453, 945, 966, 1050, 1281, 1571
Hpy166II	GTNNAC	6 cut(s)	85, 793, 829, 934, 1129, 1380
Hpy188I	TCNGA	6 cut(s)	124, 262, 452, 716, 1256, 1400
Hpy188III	TCNNGA	9 cut(s)	105, 331, 457, 584, 695, 986, 1119, 1180, 1468
Hpy8I	GTNNAC	6 cut(s)	85, 793, 829, 934, 1129, 1380
Hpy99I	CGWCG	1 cut(s)	1195
HpyAV	CCTTC	5 cut(s)	934, 1225, 1400, 1462, 1510
HpyCH4III	ACNGT	8 cut(s)	518, 805, 865, 1046, 1133, 1264, 1427, 1567
HpyCH4IV	ACGT	1 cut(s)	1090
HpyCH4V	TGCA	7 cut(s)	351, 492, 536, 639, 1270, 1278, 1380
HpyF10VI	GCNNNNNNNGC	7 cut(s)	558, 636, 737, 998, 1364, 1493, 1530
HpyF3I	CTNAG	4 cut(s)	9, 191, 449, 734
HpySE526I	ACGT	1 cut(s)	1090
Hsp92I	GRCGYC	2 cut(s)	978, 1090
HspAI	GCGC	5 cut(s)	216, 438, 630, 763, 978
KasI	GGCGCC	1 cut(s)	977
Kpn2I	TCCGGA	2 cut(s)	104, 985
Ksp22I	TGATCA	1 cut(s)	1173
KspI	CCGCGG	1 cut(s)	1477
Kzo9I	GATC	3 cut(s)	141, 459, 1173
LmnI	GCTCC	3 cut(s)	445, 1231, 1256
Lsp1109I	GCAGC	6 cut(s)	225, 229, 714, 1254, 1351, 1540
LweI	GCATC	2 cut(s)	1044, 1135
MabI	ACCWGGT	1 cut(s)	1480
MaeI	CTAG	5 cut(s)	584, 939, 1149, 1359, 1626
MaeII	ACGT	1 cut(s)	1090
MaeIII	GTNAC	6 cut(s)	374, 538, 554, 868, 875, 1427
MalI	GATC	3 cut(s)	143, 461, 1175
MboI	GATC	3 cut(s)	141, 459, 1173
MboII	GAAGA	8 cut(s)	109, 602, 1030, 1129, 1210, 1294, 1304, 1612
MhlI	GDGCHC	2 cut(s)	450, 1382
MlsI	TGGCCA	1 cut(s)	650
MluCI	AATT	6 cut(s)	154, 501, 579, 710, 1105, 1559
MluNI	TGGCCA	1 cut(s)	650
Mly113I	GGCGCC	1 cut(s)	978
MlyI	GAGTC	1 cut(s)	302
MmeI	TCCRAC	3 cut(s)	285, 399, 1234
Mox20I	TGGCCA	1 cut(s)	650
Mph1103I	ATGCAT	1 cut(s)	494
MroI	TCCGGA	2 cut(s)	104, 985
MscI	TGGCCA	1 cut(s)	650
MseI	TTAA	3 cut(s)	405, 723, 1642
MslI	CAYNNNNRTG	2 cut(s)	90, 836
Msp20I	TGGCCA	1 cut(s)	650
MspA1I	CMGCKG	3 cut(s)	705, 918, 1476
MspR9I	CCNGG	4 cut(s)	197, 1021, 1230, 1482
Mva1269I	GAATGC	2 cut(s)	353, 538
MvaI	CCWGG	2 cut(s)	1230, 1482
MvnI	CGCG	3 cut(s)	438, 630, 1476
MwoI	GCNNNNNNNGC	7 cut(s)	558, 636, 737, 998, 1364, 1493, 1530
NarI	GGCGCC	1 cut(s)	978
NciI	CCSGG	2 cut(s)	197, 1021
NcoI	CCATGG	2 cut(s)	822, 1343
NdeII	GATC	3 cut(s)	141, 459, 1173
NlaIV	GGNNCC	6 cut(s)	322, 853, 922, 979, 1258, 1486
NmuCI	GTSAC	3 cut(s)	374, 538, 868
NsiI	ATGCAT	1 cut(s)	494
NspI	RCATGY	4 cut(s)	492, 841, 1127, 1640
PciI	ACATGT	2 cut(s)	837, 1123
PcsI	WCGNNNNNNNCGW	1 cut(s)	1260
PctI	GAATGC	2 cut(s)	353, 538
PfeI	GAWTC	7 cut(s)	327, 453, 945, 966, 1050, 1281, 1571
PflFI	GACNNNGTC	1 cut(s)	292
PfoI	TCCNGGA	1 cut(s)	1228
PinAI	ACCGGT	1 cut(s)	872
PkrI	GCNGC	9 cut(s)	215, 244, 567, 704, 920, 1269, 1366, 1523, 1555
PleI	GAGTC	1 cut(s)	301
PluTI	GGCGCC	1 cut(s)	981
PpsI	GAGTC	1 cut(s)	301
PscI	ACATGT	2 cut(s)	837, 1123
Psp124BI	GAGCTC	1 cut(s)	450
Psp6I	CCWGG	2 cut(s)	1228, 1480
PspGI	CCWGG	2 cut(s)	1228, 1480
PspN4I	GGNNCC	6 cut(s)	322, 853, 922, 979, 1258, 1486
PspPI	GGNCC	4 cut(s)	336, 851, 983, 1478
PstI	CTGCAG	1 cut(s)	1280
PstNI	CAGNNNCTG	1 cut(s)	456
PsyI	GACNNNGTC	1 cut(s)	292
RsaI	GTAC	3 cut(s)	18, 794, 1076
RsaNI	GTAC	3 cut(s)	17, 793, 1075
RseI	CAYNNNNRTG	2 cut(s)	90, 836
Rsr2I	CGGWCCG	1 cut(s)	983
RsrII	CGGWCCG	1 cut(s)	983
SacI	GAGCTC	1 cut(s)	450
SacII	CCGCGG	1 cut(s)	1477
SaqAI	TTAA	3 cut(s)	405, 723, 1642
SatI	GCNGC	9 cut(s)	214, 243, 566, 703, 919, 1268, 1365, 1522, 1554
Sau3AI	GATC	3 cut(s)	141, 459, 1173
Sau96I	GGNCC	4 cut(s)	336, 851, 983, 1478
ScaI	AGTACT	1 cut(s)	18
SchI	GAGTC	1 cut(s)	302
ScrFI	CCNGG	4 cut(s)	197, 1021, 1230, 1482
SduI	GDGCHC	2 cut(s)	450, 1382
SexAI	ACCWGGT	1 cut(s)	1480
SfaNI	GCATC	2 cut(s)	1044, 1135
SfcI	CTRYAG	3 cut(s)	383, 1045, 1276
SfoI	GGCGCC	1 cut(s)	979
Sfr303I	CCGCGG	1 cut(s)	1477
SgrAI	CRCCGGYG	1 cut(s)	1529
SgrBI	CCGCGG	1 cut(s)	1477
SinI	GGWCC	3 cut(s)	336, 983, 1478
SmiMI	CAYNNNNRTG	2 cut(s)	90, 836
SmlI	CTYRAG	1 cut(s)	693
SmoI	CTYRAG	1 cut(s)	693
Sse9I	AATT	6 cut(s)	154, 501, 579, 710, 1105, 1559
SsiI	CCGC	9 cut(s)	436, 565, 705, 918, 999, 1012, 1474, 1476, 1521
SspDI	GGCGCC	1 cut(s)	977
SspMI	CTAG	5 cut(s)	584, 939, 1149, 1359, 1626
SstI	GAGCTC	1 cut(s)	450
StyD4I	CCNGG	4 cut(s)	195, 1019, 1228, 1480
StyI	CCWWGG	3 cut(s)	757, 822, 1343
TaaI	ACNGT	8 cut(s)	518, 805, 865, 1046, 1133, 1264, 1427, 1567
TaiI	ACGT	1 cut(s)	1093
TaqI	TCGA	2 cut(s)	813, 1193
TasI	AATT	6 cut(s)	154, 501, 579, 710, 1105, 1559
TatI	WGTACW	3 cut(s)	16, 792, 1074
TauI	GCSGC	3 cut(s)	568, 921, 1524
TfiI	GAWTC	7 cut(s)	327, 453, 945, 966, 1050, 1281, 1571
Tru1I	TTAA	3 cut(s)	405, 723, 1642
Tru9I	TTAA	3 cut(s)	405, 723, 1642
TscAI	CASTG	5 cut(s)	738, 868, 1060, 1228, 1326
TseFI	GTSAC	3 cut(s)	374, 538, 868
TseI	GCWGC	6 cut(s)	213, 242, 702, 1267, 1364, 1553
Tsp45I	GTSAC	3 cut(s)	374, 538, 868
TspRI	CASTG	5 cut(s)	738, 868, 1060, 1228, 1326
Tth111I	GACNNNGTC	1 cut(s)	292
VneI	GTGCAC	1 cut(s)	1378
VpaK11BI	GGWCC	3 cut(s)	336, 983, 1478
XagI	CCTNNNNNAGG	1 cut(s)	1357
XapI	RAATTY	2 cut(s)	579, 1559
XbaI	TCTAGA	1 cut(s)	583
XceI	RCATGY	4 cut(s)	492, 841, 1127, 1640
XspI	CTAG	5 cut(s)	584, 939, 1149, 1359, 1626
ZraI	GACGTC	1 cut(s)	1091
ZrmI	AGTACT	1 cut(s)	18
Zsp2I	ATGCAT	1 cut(s)	494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.