Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0003768

Physical Location & Seq

Genomic Coordinates Forward (+)

59096563 .. 59099392

Gene Structure 2830 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG274700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGGAAAAGAAGGGAAAGGCAATAGTAGGTGGAATTTCAATCATCCTTGTGGTTGGAGCAGTAGTTGGTTGTGTAGCTGTTGTTTCACATAATCGCAGCAAAGGCAAGGATAGGCTTTCGACGAGTTCCAAGGCCGTCACTGCAATGTGTGAACCAACTGATTACAAGGATGAATGCATTGACAGCCTGGCTGACGCTGCCAAGAATCAAAGCGCCACCCCAAAAGAACTTATCCGAGCCGCCATTGATTACACAATCGATAAGGTGAAAGCGGCAATAGAGAAATCAGATGGCATTATCAAAGAAGCTAAAAATGTCAATTCCACTGGAAAAATGGCCTTCGAGGATTGCAATGACTTGTTGGAATCTTCAATCGAAAGTCTTCAATATTCGCTTTTGACACTCGTTGATACTGAAATGCAAAACATGAATAATAAAGAGGTTGATCTTAAGAACTGGTTCAGTGCTGCGATATCATTTCAGGAATCATGCATTGATGGGATTCCATACGAAGACCTTAAGAACCAAATGAACGAGGCGATGCTCCATGCTTCACAACTGACCAGCAACGCCTTAGCAATTGTGTCTTCCCTTGCTCCCATCCTAGGCAAATTCAATATCTCCATGAAAACTGACAGTAAAAGTTCAACCAGTCGTCGTCTCCTGGATGTAGAAATGGAAACAGATGAACATGGCACTTTTCCAGCATGGTTCCCAGCTGCAGATAGGAAGCTATTCGCACACCACAACAAGAACAATAACAATAGCAGCAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACGACAATGACGACAACAACAACAACAGGAGAGTTACACCCAACGCAGTAGTGGCAAAAGATGGGAGTGGACAATACAAAACAATTGGTGAAGCTCTTGCTGCGTACCCAAAGGGATTCCAGGGAAGATATGTTATATATGTGAAAGCCGGGATTTACAATGAGTATGTTACTGTGGCCAAGAATCAGATCAATGTGTACATGTATGGAGATGGACCCCGGAGGACCATGATCACAGGCAGTAAAAGCTTTGCTGATGGCATCACCACCCAGGACACTGCCACATTCATCGTAATGGGGCATGGGTTCATTGGTAGAGCACTGGGATTTCAGAACACAGCCGGCCCCGAAAAGCATCAGGCAGTGGCACTCCGAGTCCAGTCGGAAATGTCAGCCTTCTTCAACTGCAGAATGGACGCCTACCAAGACACATTGTACGTGCAAGCATATCGCCAATTTTATCGCAACTGTGTCATATCCGGTACCGTTGACTTCATTTTTGGTGACTCATCAACAGTCATCCAGAATAGTTTACTCATTGTGAGGAAGCCTATGAACGGCCAAAAGAACATTGTGACTGCTCATGGAAGAAAAGATAAGCGGGAAACCACTGGTCTGGTTATCCAGAACTGCAGGATAGTCCCAGAACAGGCTTTGTTCCCAATGAGATTCCAGATTCAGTCATACTTGGGGCGGCCGTGGAAGATGTACTCGAGAACAGTTATCATGGAGTCCGAATTTGGGGACTTTATCCAACCAGCCGGTTGGCTAGAATGGCAAGGGACTTTTGGACTAGACACACTGTATTATGCAGAGTATGCAAATCGGGGTCCGGGTGCTGTAACCACAGGAAGGGTAAAATGGAAAGGATACCATGTGATTACGGACAGAAATGAGGCTCTTCAATTCACTCCGGGTAATTTTCTAACAGGAGACCAATGGTTGAAAGACACGGGTGTACCTTACTTCCTGGGTTTAAAAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

600

Amino Acids

66.68

Weight (kDa)

7.95

Isoelectric Point (pI)

28.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	45 - 194	1.9e-29	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	287 - 584	2.1e-135	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003768)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47030 AT2G47040 AT2G47050 AT3G62170 AT3G62180 AT3G62180
fragaria_vesca	FvH4_1g26360 FvH4_4g21500
malus_domestica	MD16G1136400.v1.1 MD17G1283800.v1.1
prunus_persica	Prupe.1G105400_v2.0.a1 Prupe.3G003700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0486131 RchiOBHm_Chr4g0427511
rosa_laevigata	RLG00000007232 RLG00000023101
rosa_multiflora	Rmu_sc0001006.1_g000002 Rmu_sc0009627.1_g000001 Rmu_sc0017895.1_g000001 Rmu_sc0017895.1_g000002
rosa_roxburghii	Rroxscaffold_5G00369150 Rroxscaffold_6G00396030
rosa_rugosa	Rorug03G0223700 Rorug04G0218200
rosa_samantha	Rh3AG273500 Rh3BG308600 Rh3CG307100 Rh3DG303400 Rh4AG274700 Rh4BG280500 Rh4CG295800 Rh4DG277900
rosa_wichuraiana	Rw3G024240 Rw4G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1298
AccB1I	GGYRCC	1 cut(s)	1298
AciI	CCGC	4 cut(s)	240, 272, 1417, 1510
AcoI	YGGCCR	3 cut(s)	993, 1375, 1511
AcsI	RAATTY	3 cut(s)	33, 611, 1553
AcyI	GRCGYC	1 cut(s)	1233
AfaI	GTAC	6 cut(s)	923, 1016, 1253, 1300, 1526, 1776
AfiI	CCNNNNNNNGG	5 cut(s)	605, 1373, 1465, 1557, 1668
AflII	CTTAAG	2 cut(s)	449, 518
AflIII	ACRYGT	1 cut(s)	1017
AgsI	TTSAA	8 cut(s)	39, 372, 386, 616, 648, 1219, 1721, 1762
AjnI	CCWGG	5 cut(s)	186, 663, 936, 1086, 1785
AluBI	AGCT	6 cut(s)	77, 308, 719, 733, 911, 1065
AluI	AGCT	6 cut(s)	77, 308, 719, 733, 911, 1065
Alw21I	GWGCWC	1 cut(s)	1138
Alw26I	GTCTC	2 cut(s)	665, 1743
Ama87I	CYCGRG	1 cut(s)	1528
AoxI	GGCC	6 cut(s)	132, 336, 993, 1159, 1375, 1511
ApeKI	GCWGC	6 cut(s)	96, 197, 467, 719, 768, 917
ApoI	RAATTY	3 cut(s)	33, 611, 1553
ArsI	GACNNNNNNTTYG	2 cut(s)	1298, 1330
Asp700I	GAANNNNTTC	3 cut(s)	381, 458, 734
Asp718I	GGTACC	1 cut(s)	1298
AspA2I	CCTAGG	1 cut(s)	604
AspLEI	GCGC	1 cut(s)	215
AspS9I	GGNCC	4 cut(s)	1031, 1041, 1160, 1646
AsuC2I	CCSGG	4 cut(s)	967, 1036, 1650, 1731
AsuHPI	GGTGA	4 cut(s)	277, 917, 1072, 1331
AvaI	CYCGRG	1 cut(s)	1528
AvaII	GGWCC	3 cut(s)	1031, 1041, 1646
AvrII	CCTAGG	1 cut(s)	604
BaeI	ACNNNNGTAYC	2 cut(s)	1758, 1791
BalI	TGGCCA	1 cut(s)	995
BanI	GGYRCC	1 cut(s)	1298
BbsI	GAAGAC	3 cut(s)	374, 519, 579
Bbv12I	GWGCWC	1 cut(s)	1138
BbvI	GCAGC	6 cut(s)	108, 184, 454, 706, 780, 904
BccI	CCATC	6 cut(s)	284, 491, 608, 872, 1022, 1067
BceAI	ACGGC	3 cut(s)	119, 1390, 1498
BcgI	CGANNNNNNTGC	2 cut(s)	1247, 1281
BciT130I	CCWGG	5 cut(s)	188, 665, 938, 1088, 1787
BciVI	GTATCC	1 cut(s)	1679
BclI	TGATCA	1 cut(s)	1047
BcnI	CCSGG	4 cut(s)	967, 1036, 1650, 1731
BcoDI	GTCTC	2 cut(s)	665, 1743
BfaI	CTAG	4 cut(s)	605, 1586, 1610, 1801
BfmI	CTRYAG	3 cut(s)	720, 1222, 1447
BfoI	RGCGCY	1 cut(s)	216
BfrI	CTTAAG	2 cut(s)	449, 518
BfuI	GTATCC	1 cut(s)	1679
BisI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 918, 1511
BlnI	CCTAGG	1 cut(s)	604
BlsI	GCNGC	9 cut(s)	98, 199, 241, 274, 469, 721, 770, 919, 1512
Bme1390I	CCNGG	9 cut(s)	188, 665, 938, 967, 1036, 1088, 1650, 1731, 1787
Bme18I	GGWCC	3 cut(s)	1031, 1041, 1646
BmeT110I	CYCGRG	1 cut(s)	1528
BmgT120I	GGNCC	4 cut(s)	1031, 1041, 1160, 1646
BmiI	GGNNCC	5 cut(s)	713, 1033, 1162, 1300, 1647
BmrFI	CCNGG	9 cut(s)	188, 665, 938, 967, 1036, 1088, 1650, 1731, 1787
BmrI	ACTGGG	1 cut(s)	1148
BmsI	GCATC	3 cut(s)	531, 1086, 1180
BmuI	ACTGGG	1 cut(s)	1148
BpiI	GAAGAC	3 cut(s)	374, 519, 579
Bpu10I	CCTNAGC	1 cut(s)	574
BpuMI	CCSGG	4 cut(s)	967, 1036, 1650, 1731
Bsa29I	ATCGAT	1 cut(s)	258
BsaAI	YACGTR	1 cut(s)	1255
BsaHI	GRCGYC	1 cut(s)	1233
BsaI	GGTCTC	1 cut(s)	1743
BsaJI	CCNNGG	7 cut(s)	129, 604, 937, 1034, 1086, 1514, 1786
BsaWI	WCCGGW	1 cut(s)	1295
BsaXI	ACNNNNNCTCC	2 cut(s)	48, 78
Bsc4I	CCNNNNNNNGG	5 cut(s)	605, 1373, 1465, 1557, 1668
Bse118I	RCCGGY	2 cut(s)	1157, 1577
Bse1I	ACTGG	6 cut(s)	331, 461, 651, 1143, 1195, 1432
Bse3DI	GCAATG	2 cut(s)	150, 358
BseBI	CCWGG	5 cut(s)	188, 665, 938, 1088, 1787
BseCI	ATCGAT	1 cut(s)	258
BseDI	CCNNGG	7 cut(s)	129, 604, 937, 1034, 1086, 1514, 1786
BseGI	GGATG	5 cut(s)	42, 175, 600, 673, 1335
BseLI	CCNNNNNNNGG	5 cut(s)	605, 1373, 1465, 1557, 1668
BseMI	GCAATG	2 cut(s)	150, 358
BseNI	ACTGG	6 cut(s)	331, 461, 651, 1143, 1195, 1432
BseX3I	CGGCCG	1 cut(s)	1511
BseXI	GCAGC	6 cut(s)	108, 184, 454, 706, 780, 904
BseYI	CCCAGC	1 cut(s)	715
Bsh1285I	CGRYCG	1 cut(s)	1514
BshFI	GGCC	6 cut(s)	134, 338, 995, 1161, 1377, 1513
BshNI	GGYRCC	1 cut(s)	1298
BshVI	ATCGAT	1 cut(s)	258
BsiEI	CGRYCG	1 cut(s)	1514
BsiHKAI	GWGCWC	1 cut(s)	1138
BsiHKCI	CYCGRG	1 cut(s)	1528
BsiSI	CCGG	7 cut(s)	966, 1036, 1158, 1296, 1578, 1649, 1730
BslFI	GGGAC	3 cut(s)	1442, 1574, 1612
BslI	CCNNNNNNNGG	5 cut(s)	605, 1373, 1465, 1557, 1668
BsmAI	GTCTC	2 cut(s)	665, 1743
BsmBI	CGTCTC	1 cut(s)	665
BsmFI	GGGAC	3 cut(s)	1442, 1574, 1612
BsmI	GAATGC	1 cut(s)	179
BsnI	GGCC	6 cut(s)	134, 338, 995, 1161, 1377, 1513
Bso31I	GGTCTC	1 cut(s)	1743
BsoBI	CYCGRG	1 cut(s)	1528
Bsp1286I	GDGCHC	1 cut(s)	1138
Bsp1407I	TGTACA	1 cut(s)	1014
Bsp143I	GATC	3 cut(s)	445, 1005, 1047
BspACI	CCGC	4 cut(s)	240, 272, 1417, 1510
BspANI	GGCC	6 cut(s)	134, 338, 995, 1161, 1377, 1513
BspDI	ATCGAT	1 cut(s)	258
BspLI	GGNNCC	5 cut(s)	713, 1033, 1162, 1300, 1647
BspMAI	CTGCAG	3 cut(s)	724, 1226, 1451
BspQI	GCTCTTC	1 cut(s)	1722
BspT107I	GGYRCC	1 cut(s)	1298
BspTI	CTTAAG	2 cut(s)	449, 518
BspTNI	GGTCTC	1 cut(s)	1743
BsrDI	GCAATG	2 cut(s)	150, 358
BsrFI	RCCGGY	2 cut(s)	1157, 1577
BsrGI	TGTACA	1 cut(s)	1014
BsrI	ACTGG	6 cut(s)	331, 461, 651, 1143, 1195, 1432
BssAI	RCCGGY	2 cut(s)	1157, 1577
BssECI	CCNNGG	7 cut(s)	129, 604, 937, 1034, 1086, 1514, 1786
BssMI	GATC	3 cut(s)	445, 1005, 1047
BssNI	GRCGYC	1 cut(s)	1233
BssT1I	CCWWGG	2 cut(s)	129, 604
Bst2UI	CCWGG	5 cut(s)	188, 665, 938, 1088, 1787
Bst4CI	ACNGT	7 cut(s)	638, 991, 1286, 1303, 1333, 1537, 1620
Bst6I	CTCTTC	1 cut(s)	1722
BstACI	GRCGYC	1 cut(s)	1233
BstAFI	CTTAAG	2 cut(s)	449, 518
BstAPI	GCANNNNNTGC	1 cut(s)	1634
BstAUI	TGTACA	1 cut(s)	1014
BstBAI	YACGTR	1 cut(s)	1255
BstC8I	GCNNGC	2 cut(s)	1159, 1260
BstDEI	CTNAG	1 cut(s)	574
BstDSI	CCRYGG	1 cut(s)	1514
BstF5I	GGATG	5 cut(s)	42, 175, 600, 673, 1335
BstH2I	RGCGCY	1 cut(s)	216
BstHHI	GCGC	1 cut(s)	215
BstKTI	GATC	3 cut(s)	448, 1008, 1050
BstMAI	GTCTC	2 cut(s)	665, 1743
BstMBI	GATC	3 cut(s)	445, 1005, 1047
BstMCI	CGRYCG	1 cut(s)	1514
BstMWI	GCNNNNNNNGC	9 cut(s)	102, 140, 183, 197, 869, 917, 1062, 1591, 1634
BstNI	CCWGG	5 cut(s)	188, 665, 938, 1088, 1787
BstNSI	RCATGY	1 cut(s)	1021
BstSCI	CCNGG	9 cut(s)	186, 663, 936, 965, 1034, 1086, 1648, 1729, 1785
BstSFI	CTRYAG	3 cut(s)	720, 1222, 1447
BstV1I	GCAGC	6 cut(s)	108, 184, 454, 706, 780, 904
BstV2I	GAAGAC	3 cut(s)	374, 519, 579
BstXI	CCANNNNNNTGG	2 cut(s)	1432, 1581
BstZI	CGGCCG	1 cut(s)	1511
Bsu15I	ATCGAT	1 cut(s)	258
BsuI	GTATCC	1 cut(s)	1679
BsuRI	GGCC	6 cut(s)	134, 338, 995, 1161, 1377, 1513
BsuTUI	ATCGAT	1 cut(s)	258
BtgI	CCRYGG	1 cut(s)	1514
BtgZI	GCGATG	1 cut(s)	554
BtsCI	GGATG	5 cut(s)	42, 175, 600, 673, 1335
BtsI	GCAGTG	3 cut(s)	138, 1092, 1185
BtsIMutI	CAGTG	8 cut(s)	138, 324, 469, 1092, 1136, 1185, 1425, 1616
Cac8I	GCNNGC	2 cut(s)	1159, 1260
CfoI	GCGC	1 cut(s)	215
Cfr10I	RCCGGY	2 cut(s)	1157, 1577
Cfr13I	GGNCC	4 cut(s)	1031, 1041, 1160, 1646
ClaI	ATCGAT	1 cut(s)	258
CseI	GACGC	2 cut(s)	203, 1241
Csp6I	GTAC	6 cut(s)	922, 1015, 1252, 1299, 1525, 1775
CviQI	GTAC	6 cut(s)	922, 1015, 1252, 1299, 1525, 1775
DdeI	CTNAG	1 cut(s)	574
DpnI	GATC	3 cut(s)	447, 1007, 1049
DpnII	GATC	3 cut(s)	445, 1005, 1047
DraI	TTTAAA	1 cut(s)	1794
EaeI	YGGCCR	3 cut(s)	993, 1375, 1511
EagI	CGGCCG	1 cut(s)	1511
Eam1104I	CTCTTC	1 cut(s)	1722
EarI	CTCTTC	1 cut(s)	1722
EclXI	CGGCCG	1 cut(s)	1511
Eco130I	CCWWGG	2 cut(s)	129, 604
Eco31I	GGTCTC	1 cut(s)	1743
Eco32I	GATATC	1 cut(s)	474
Eco47I	GGWCC	3 cut(s)	1031, 1041, 1646
Eco52I	CGGCCG	1 cut(s)	1511
Eco88I	CYCGRG	1 cut(s)	1528
EcoRII	CCWGG	5 cut(s)	186, 663, 936, 1086, 1785
EcoRV	GATATC	1 cut(s)	474
EcoT14I	CCWWGG	2 cut(s)	129, 604
EcoT22I	ATGCAT	2 cut(s)	179, 494
ErhI	CCWWGG	2 cut(s)	129, 604
Esp3I	CGTCTC	1 cut(s)	665
FaqI	GGGAC	3 cut(s)	1442, 1574, 1612
FauI	CCCGC	1 cut(s)	1410
FbaI	TGATCA	1 cut(s)	1047
Fnu4HI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 918, 1511
FokI	GGATG	5 cut(s)	29, 182, 587, 680, 1322
Fsp4HI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 918, 1511
FspBI	CTAG	4 cut(s)	605, 1586, 1610, 1801
GlaI	GCGC	1 cut(s)	214
GluI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 918, 1511
GsaI	CCCAGC	1 cut(s)	719
HaeII	RGCGCY	1 cut(s)	216
HaeIII	GGCC	6 cut(s)	134, 338, 995, 1161, 1377, 1513
HapII	CCGG	7 cut(s)	966, 1036, 1158, 1296, 1578, 1649, 1730
HgaI	GACGC	2 cut(s)	203, 1241
HhaI	GCGC	1 cut(s)	215
Hin1I	GRCGYC	1 cut(s)	1233
Hin6I	GCGC	1 cut(s)	213
HinP1I	GCGC	1 cut(s)	213
HincII	GTYRAC	1 cut(s)	1306
HindII	GTYRAC	1 cut(s)	1306
HindIII	AAGCTT	1 cut(s)	1063
HpaII	CCGG	7 cut(s)	966, 1036, 1158, 1296, 1578, 1649, 1730
HphI	GGTGA	4 cut(s)	277, 917, 1072, 1331
Hpy166II	GTNNAC	6 cut(s)	152, 887, 1015, 1306, 1349, 1775
Hpy188I	TCNGA	7 cut(s)	236, 289, 1005, 1149, 1190, 1201, 1552
Hpy188III	TCNNGA	5 cut(s)	482, 1339, 1441, 1489, 1530
Hpy8I	GTNNAC	6 cut(s)	152, 887, 1015, 1306, 1349, 1775
Hpy99I	CGWCG	2 cut(s)	124, 660
HpyAV	CCTTC	4 cut(s)	4, 349, 1222, 1662
HpyCH4III	ACNGT	7 cut(s)	638, 991, 1286, 1303, 1333, 1537, 1620
HpyCH4IV	ACGT	1 cut(s)	1254
HpyF10VI	GCNNNNNNNGC	9 cut(s)	102, 140, 183, 197, 869, 917, 1062, 1591, 1634
HpyF3I	CTNAG	1 cut(s)	574
HpySE526I	ACGT	1 cut(s)	1254
Hsp92I	GRCGYC	1 cut(s)	1233
HspAI	GCGC	1 cut(s)	213
KpnI	GGTACC	1 cut(s)	1302
KroI	GCCGGC	1 cut(s)	1157
KroNI	GCCGGC	1 cut(s)	1159
Ksp22I	TGATCA	1 cut(s)	1047
Kzo9I	GATC	3 cut(s)	445, 1005, 1047
LguI	GCTCTTC	1 cut(s)	1722
LmnI	GCTCC	3 cut(s)	56, 549, 601
Lsp1109I	GCAGC	6 cut(s)	108, 184, 454, 706, 780, 904
LweI	GCATC	3 cut(s)	531, 1086, 1180
MaeI	CTAG	4 cut(s)	605, 1586, 1610, 1801
MaeII	ACGT	1 cut(s)	1254
MaeIII	GTNAC	6 cut(s)	136, 850, 985, 1319, 1390, 1657
MalI	GATC	3 cut(s)	447, 1007, 1049
MboI	GATC	3 cut(s)	445, 1005, 1047
MboII	GAAGA	9 cut(s)	360, 374, 524, 579, 954, 1207, 1416, 1531, 1709
MfeI	CAATTG	2 cut(s)	579, 900
MhlI	GDGCHC	1 cut(s)	1138
MlsI	TGGCCA	1 cut(s)	995
MluCI	AATT	9 cut(s)	33, 319, 579, 611, 900, 1271, 1553, 1721, 1735
MluNI	TGGCCA	1 cut(s)	995
MlyI	GAGTC	3 cut(s)	1200, 1316, 1556
MmeI	TCCRAC	4 cut(s)	34, 342, 1179, 1594
MnlI	CCTC	6 cut(s)	337, 433, 529, 1032, 1353, 1705
Mox20I	TGGCCA	1 cut(s)	995
Mph1103I	ATGCAT	2 cut(s)	179, 494
MroNI	GCCGGC	1 cut(s)	1157
MroXI	GAANNNNTTC	3 cut(s)	381, 458, 734
MscI	TGGCCA	1 cut(s)	995
MseI	TTAA	3 cut(s)	450, 519, 1793
MslI	CAYNNNNRTG	3 cut(s)	47, 143, 1109
Msp20I	TGGCCA	1 cut(s)	995
MspA1I	CMGCKG	1 cut(s)	719
MspCI	CTTAAG	2 cut(s)	449, 518
MspI	CCGG	7 cut(s)	966, 1036, 1158, 1296, 1578, 1649, 1730
MspR9I	CCNGG	9 cut(s)	188, 665, 938, 967, 1036, 1088, 1650, 1731, 1787
MunI	CAATTG	2 cut(s)	579, 900
Mva1269I	GAATGC	1 cut(s)	179
MvaI	CCWGG	5 cut(s)	188, 665, 938, 1088, 1787
MwoI	GCNNNNNNNGC	9 cut(s)	102, 140, 183, 197, 869, 917, 1062, 1591, 1634
NaeI	GCCGGC	1 cut(s)	1159
NciI	CCSGG	4 cut(s)	967, 1036, 1650, 1731
NdeII	GATC	3 cut(s)	445, 1005, 1047
NgoMIV	GCCGGC	1 cut(s)	1157
NlaIV	GGNNCC	5 cut(s)	713, 1033, 1162, 1300, 1647
NmuCI	GTSAC	3 cut(s)	136, 1319, 1390
NsiI	ATGCAT	2 cut(s)	179, 494
NspI	RCATGY	1 cut(s)	1021
PaeR7I	CTCGAG	1 cut(s)	1528
PciI	ACATGT	1 cut(s)	1017
PciSI	GCTCTTC	1 cut(s)	1722
PcsI	WCGNNNNNNNCGW	1 cut(s)	825
PctI	GAATGC	1 cut(s)	179
PdiI	GCCGGC	1 cut(s)	1159
PdmI	GAANNNNTTC	3 cut(s)	381, 458, 734
PfeI	GAWTC	8 cut(s)	205, 365, 485, 502, 933, 1000, 1485, 1492
PfoI	TCCNGGA	1 cut(s)	663
PkrI	GCNGC	9 cut(s)	98, 199, 241, 274, 469, 721, 770, 919, 1512
PleI	GAGTC	3 cut(s)	1199, 1316, 1555
PpsI	GAGTC	3 cut(s)	1199, 1316, 1555
Ppu21I	YACGTR	1 cut(s)	1255
PscI	ACATGT	1 cut(s)	1017
Psp6I	CCWGG	5 cut(s)	186, 663, 936, 1086, 1785
PspFI	CCCAGC	1 cut(s)	715
PspGI	CCWGG	5 cut(s)	186, 663, 936, 1086, 1785
PspN4I	GGNNCC	5 cut(s)	713, 1033, 1162, 1300, 1647
PspPI	GGNCC	4 cut(s)	1031, 1041, 1160, 1646
PstI	CTGCAG	3 cut(s)	724, 1226, 1451
PvuII	CAGCTG	1 cut(s)	719
RsaI	GTAC	6 cut(s)	923, 1016, 1253, 1300, 1526, 1776
RsaNI	GTAC	6 cut(s)	922, 1015, 1252, 1299, 1525, 1775
RseI	CAYNNNNRTG	3 cut(s)	47, 143, 1109
SapI	GCTCTTC	1 cut(s)	1722
SaqAI	TTAA	3 cut(s)	450, 519, 1793
SatI	GCNGC	9 cut(s)	97, 198, 240, 273, 468, 720, 769, 918, 1511
Sau3AI	GATC	3 cut(s)	445, 1005, 1047
Sau96I	GGNCC	4 cut(s)	1031, 1041, 1160, 1646
SchI	GAGTC	3 cut(s)	1200, 1316, 1556
ScrFI	CCNGG	9 cut(s)	188, 665, 938, 967, 1036, 1088, 1650, 1731, 1787
SduI	GDGCHC	1 cut(s)	1138
SfaNI	GCATC	3 cut(s)	531, 1086, 1180
SfcI	CTRYAG	3 cut(s)	720, 1222, 1447
Sfr274I	CTCGAG	1 cut(s)	1528
SinI	GGWCC	3 cut(s)	1031, 1041, 1646
SlaI	CTCGAG	1 cut(s)	1528
SmiMI	CAYNNNNRTG	3 cut(s)	47, 143, 1109
SmlI	CTYRAG	3 cut(s)	449, 518, 1528
SmoI	CTYRAG	3 cut(s)	449, 518, 1528
Sse9I	AATT	9 cut(s)	33, 319, 579, 611, 900, 1271, 1553, 1721, 1735
SsiI	CCGC	4 cut(s)	240, 272, 1417, 1510
SspI	AATATT	1 cut(s)	389
SspMI	CTAG	4 cut(s)	605, 1586, 1610, 1801
StyD4I	CCNGG	9 cut(s)	186, 663, 936, 965, 1034, 1086, 1648, 1729, 1785
StyI	CCWWGG	2 cut(s)	129, 604
TaaI	ACNGT	7 cut(s)	638, 991, 1286, 1303, 1333, 1537, 1620
TaiI	ACGT	1 cut(s)	1257
TaqI	TCGA	5 cut(s)	119, 258, 342, 375, 1529
TasI	AATT	9 cut(s)	33, 319, 579, 611, 900, 1271, 1553, 1721, 1735
TatI	WGTACW	2 cut(s)	1014, 1524
TauI	GCSGC	3 cut(s)	242, 275, 1513
TfiI	GAWTC	8 cut(s)	205, 365, 485, 502, 933, 1000, 1485, 1492
Tru1I	TTAA	3 cut(s)	450, 519, 1793
Tru9I	TTAA	3 cut(s)	450, 519, 1793
TscAI	CASTG	8 cut(s)	145, 331, 469, 1099, 1143, 1185, 1432, 1623
TseFI	GTSAC	3 cut(s)	136, 1319, 1390
TseI	GCWGC	6 cut(s)	96, 197, 467, 719, 768, 917
Tsp45I	GTSAC	3 cut(s)	136, 1319, 1390
TspDTI	ATGAA	9 cut(s)	186, 443, 545, 641, 702, 1093, 1114, 1300, 1385
TspGWI	ACGGA	1 cut(s)	1715
TspRI	CASTG	8 cut(s)	145, 331, 469, 1099, 1143, 1185, 1432, 1623
Vha464I	CTTAAG	2 cut(s)	449, 518
VpaK11BI	GGWCC	3 cut(s)	1031, 1041, 1646
XapI	RAATTY	3 cut(s)	33, 611, 1553
XceI	RCATGY	1 cut(s)	1021
XcmI	CCANNNNNNNNNTGG	2 cut(s)	331, 865
XhoI	CTCGAG	1 cut(s)	1528
XmaJI	CCTAGG	1 cut(s)	604
XmnI	GAANNNNTTC	3 cut(s)	381, 458, 734
XspI	CTAG	4 cut(s)	605, 1586, 1610, 1801
Zsp2I	ATGCAT	2 cut(s)	179, 494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.