AT3G01460

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004967
Physical Location & Seq
Forward (+)
173190 .. 182454
9265 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G01460.1

Sequence Viewer

Length: 6531 bp
ATGGAACCCACTGATTCTACTAACGAGCAACTCGGAGACACTAAGACCGCCGCTGTCAAGGAAGAGAGTCGCTCCTTTCTCGGCATCGATCTCAACGAAATCCCTACCGGCGCTACTCTCGGCGGTGGTTGCACCGCTGGTCAGGATGACGACGGCGAATATGAACCTGTTGAAGTTGTTAGGTCAATTCACGATAACCCGGACCCAGCCCCTGGAGCCCCTGCCGAGGTTCCTGAACCGGATCGGGATGCTTCGTGCGGCGCGTGTGGAAGACCTGAGTCTATAGAGCTCGTTGTAGTCTGCGATGCCTGTGAGCGAGGCTTTCATATGTCTTGTGTCAACGATGGAGTTGAGGCGGCTCCTTCCGCCGATTGGATGTGCAGCGACTGTCGTACTGGCGGCGAGAGGAGCAAACTGTGGCCGTTGGGGGTTAAGTCCAAGCTCATTCTGGACATGAACGCCTCGCCGCCCAGTGATGCTGAGGGATACGGAGCTGAGGAGACGTCTGATTCGAGAAAGCATATGCTCGCCAGCAGCTCTTGCATTGGAAACTCTTTTGATTATGCAATGATGCATTCAAGCTTCTCAAGTCTCGGTAGAGGACATGCTAGTCTCGAAGCTTCAGGGTTAATGTCTCGTAATACTAAAATGAGTATGGATGCATTGGGTTCACATAATCTAGGTTTTGGATTCCCATTAAACTTGAACAATAGTAGTTTGCCCATGAGATTTCCATCCTTGGATCCAAGTGAGTTGTTTCTGCAAAATCTCAGGCATTTCATATCTGAAAGGCATGGAGTATTGGAAGATGGCTGGCGTGTCGAATTCAGACAACCTTTAAATGGTTATCAGTTATGTGCAGTGTATTGTGCTCCGAATGGAAAAACATTTAGTTCAATACAAGAAGTTGCTTGTTATCTGGGCTTGGCAATTAATGGTAACTACAGCTGTATGGATGCTGAAATCAGGAATGAGAATTCTCTTCTTCAAGAAAGATTGCATACGCCCAAGAGAAGAAAGACATCAAGATGGCCAAACAATGGTTTCCCTGAGCAAAAGGGTAGTTCAGTGAGTGCTCAACTCAGGCGTTTTCCATTCAATGGTCAGACCATGTCTCCTTTCGCCGTTAAATCTGGTACTCATTTTCAGGCTGGTGGTTCTCTTAGCTCTGGAAATAATGGATGCGGTTGTGAGGAAGCTAAGAATGGATGTCCGATGCAGTTTGAAGATTTCTTTGTTCTGTCGCTTGGACGAATTGACATAAGACAGTCTTACCATAATGTCAACGTGATTTATCCAATAGGATATAAGTCCTGCTGGCATGACAAGATCACGGGGTCACTATTTACATGTGAAGTATCTGATGGCAATTCTGGTCCCATTTTCAAGGTTACACGGTCACCATGCTCGAAATCATTTATTCCAGCTGGATCAACTGTCTTCTCCTGCCCAAAGATTGATGAAATGGTGGAACAGAACAGTGACAAACTTAGTAATCGTAGAGATAGTACTCAAGAGCGTGATGATGACGCTAGTGTTGAGATCCTTCTTTCGGAACACTGCCCACCTCTTGGAGATGATATATTGTCTTGTTTACGTGAGAAGAGTTTCTCCAAGACAGTCAATTCCCTGCGCTCAGAGGTTGATTCTTCTCGAGTAGATTTTGATAAAAATTTATCCTATGATCAGGACCATGGGGTTGAAATTGGTGACATTGTTGTGGAAGAAGATTCATTGTCTGATGCATGGAAAAAAGTGTCTCAAAAACTTGTTGATGCATGTTCAATTGTACTGAAGCAGAAGGGTACCCTGAATTTCCTATGTAAGCATGTTGACAGAGAAACAAGTGAAATCAACTGGGATACCATGAATGAGAAAGACAATGTAATTTTATCGTTGTCAAAATTTTGCTGTTCGTTGGCTCCTTGCAGTGTCACGTGTGGTGAAAAGGATAAAAGCGAATTTGCAGCAGTAGTTGATGCTTTGTCAAGGTGGCTCGATCAAAACAGATTTGGACTTGATGCAGATTTTGTACAGGAAATGATTGAACATATGCCTGGTGCCGAATCATGTACGAATTATAGGACTCTGAAGAGTAGAAGTTCTTCTTCTGTTCCTATAACTGTAGCGGAAGGAGCGCTAGTGGTCAAACCAAAAGGTGGGGAAAATGTCAAGGACGAAGTTTTCGGTGAGATTTCTCGGAAAGCCAAGAAGCCTAAACTAAATGGTGGTCATGGTGTCAGAAATCTACACCCTCCTCCTGGGAGGCCAATGTGTTTGAGGCTCCCTCCTGGGCTTGTTGGTGACTTCCTTCAGGTATCTGAAGTGTTCTGGCGTTTCCATGAAATTTTGGGTTTTGAAGAGGCTTTCTCACCTGAAAACCTTGAACAGGAGCTTATCAATCCAGTGTTTGATGGTTTGTTTCTTGATAAACCTGGGAAAGATGATAAGAGAAGTGAGATTAACTTTACTGATAAGGATTCTACAGCTACTAAACTTTTTTCTTTGTTCGATGAATCTCGCCAACCTTTTCCTGCAAAAAATACCTCTGCTTCTGAACTAAAGGAGAAAAAGGCAGGGGATTCTTCTGATTTTAAGATTTCAGATTCCTCTCGTGGGTCGTGTGTGGGTGCACTTCTAACAAGGGCTCACATTTCGCTTCTGCAAGTGCTAATATGTGAGCTGCAATCCAAGGTAGCTGCATTTGTTGATCCAAACTTTGATTCTGGCGAATCGAGATCCAGACGAGGACGAAAAAAGGATGACAGTACACTTTCTGCTAAAAGAAATAAGCTGCATATGCTTCCTGTTAATGAGTTCACTTGGCCTGAATTGGCCCGTAGGTACATCTTGTCTCTTTTATCCATGGATGGGAACCTCGAATCAGCAGAGATTGCTGCACGTGAAAGTGGTAAGGTATTCCGTTGCTTACAAGGGGATGGTGGTTTGCTTTGCGGCTCGCTTACAGGAGTGGCTGGGATGGAAGCAGATTCAATGTTACTTGCAGAGGCTATTAAGAAAATATCTGGTTCGTTGACAAGCGAAAATGATGTTCTTTCTGTGGAAGATGATGATTCCGATGGCCTTGATGCTACTGAGACAAACACTTGCAGTGGTGATATTCCAGAGTGGGCTCAGGTTTTGGAACCTGTGAAAAAGCTTCCAACAAATGTTGGGACTAGAATCAGAAAGTGTGTCTATGAAGCTTTAGAGAGAAATCCACCAGAGTGGGCAAAGAAGATATTGGAGCATTCTATCAGTAAAGAAATATATAAAGGCAATGCATCAGGACCAACAAAGAAAGCTGTCCTCTCATTGCTAGCGGATATTCGAGGTGGAGACTTGGTGCAGAGGTCTATTAAAGGAACCAAAAAGCGGACATATATAAGTGTATCTGATGTCATTATGAAGAAATGCCGTGCTGTATTGCGTGGTGTTGCAGCTGCAGACGAGGATAAAGTCCTTTGCACTTTACTGGGAAGAAAGTTACTGAATTCCAGTGATAATGATGATGACGGGCTCCTGGGATCACCTGCAATGGTTTCGCGTCCCTTAGACTTCAGAACTATTGATTTGAGGTTGGCTGCTGGTGCGTATGACGGATCAACTGAAGCTTTTCTTGAAGATGTTCTTGAGCTGTGGAGTAGTATACGTGTTATGTATGCAGATCAGCCCGATTGTGTGGACCTGGTTGCAACATTGTCTGAAAAATTCAAGTCGTTATACGAGGCTGAGGTTGTACCACTTGTTCAGAAACTTAAGGACTACAGGAAATTGGAATGCCTAAGTGCAGAGATGAAGAAGGAAATTAAGGACATAGTTGTTTCAGTAAATAAGCTTCCCAAGGCCCCGTGGGATGAGGGGGTATGTAAAGTATGTGGCGTTGACAAAGATGATGACAGTGTTCTCTTGTGTGATACATGCGATGCTGAGTATCACACATATTGTTTAAATCCACCTCTTATTAGAATTCCTGATGGAAATTGGTATTGTCCATCTTGTGTCATTGCCAAGCGCATGGCTCAAGAGGCTTTGGAATCTTACAAACTAGTTAGGCGGCGGAAAGGTAGAAAGTATCAGGGGGAACTCACCCGAGCTTCTATGGAACTGACTGCTCACCTGGCAGATGTGATGGAAGAAAAGGACTACTGGGAGTTTAGTGCTGAGGAGAGAATCTTGCTGCTTAAGCTTCTATGCGATGAACTGCTTAGTTCATCTCTTGTCCATCAACACCTTGAGCAGTGTGCCGAAGCAATAATTGAAATGCAGCAGAAGTTACGCTCTCTTTCCTCAGAATGGAAAAACGCAAAAATGCGGCAAGAATTCCTGACGGCTAAACTGGCAAAGGTTGAACCGAGTATTCTGAAGGAAGTGGGCGAACCACATAATTCAAGCTACTTTGCAGACCAAATGGGATGTGATCCACAACCACAGGAGGGCGTTGGAGACGGAGTTACTCGTGATGATGAGACTTCCTCTACTGCATATCTTAACAAGAATCAAGGTAAATCTCCACTTGAAACCGATACTCAACCCGGAGAGTCGCATGTTAATTTCGGTGAGAGCAAAATTTCCTCCCCGGAAACAATATCATCCCCTGGGAGGCATGAGCTACCTATAGCAGATACCTCTCCTCTTGTAACAGATAATCTGCCTGAAAAAGATACCTCGGAGACCTTGCTTAAGTCAGTTGGAAGGAATCATGAAACACATTCACCAAATTCCAATGCAGTAGAATTGCCGACAGCTCATGATGCATCTTCTCAGGCTTCCCAAGAGTTGCAGGCTTGTCAGCAGGATTTGAGTGCCACTAGTAATGAAATTCAGAATCTTCAGCAATCAATTAGAAGCATAGAATCACAGCTTCTAAAGCAATCTATACGGAGAGATTTTCTGGGAACCGATGCTAGTGGTCGGTTATATTGGGGTTGCTGCTTCCCAGATGAAAATCCTCGTATATTGGTTGATGGAAGCATATCTTTGCAGAAACCTGTTCAAGCCGATTTGATAGGTTCAAAAGTCCCCTCTCCGTTTCTCCATACCGTTGACCATGGAAGACTAAGGCTTTCACCCTGGACGTATTATGAAACTGAAACCGAGATCAGTGAGCTTGTCCAATGGCTTCATGATGATGATCTGAAAGAAAGAGACCTGAGAGAGTCTATTTTGTGGTGGAAAAGGTTACGATATGGAGACGTTCAAAAGGAAAAGAAACAAGCTCAGAATTTATCTGCTCCGGTATTTGCTACGGGTCTTGAGACCAAGGCTGCCATGTCAATGGAGAAGAGATATGGTCCATGCATCAAACTGGAGATGGAAACCTTAAAAAAACGGGGGAAGAAGACAAAGGTTGCAGAGCGAGAGAAATTGTGTAGATGCGAATGCTTGGAATCCATTTTGCCATCTATGATTCACTGCCTCATATGCCATAAAACATTCGCAAGTGATGATGAGTTTGAGGATCACACTGAGAGTAAGTGTATTCCTTATTCATTAGCAACTGAAGAAGGCAAGGACATCTCTGATTCTTCAAAAGCCAAAGAAAGTCTGAAATCCGATTATCTTAATGTAAAGTCTAGTGCCGGCAAAGATGTAGCTGAAATATCCAATGTTTCTGAACTTGATTCTGGGTTGATAAGATATCAAGAAGAAGAATCTATTTCCCCATACCATTTTGAGGAGATCTGTTCCAAGTTTGTGACAAAGGATTGCAACAGAGATTTGGTTAAAGAGATCGGTCTGATCAGTTCAAATGGCATTCCAACATTTCTTCCATCGTCATCTACTCATCTTAACGACTCCGTGCTCATCTCTGCCAAATCCAATAAGCCAGATGGTGGTGATTCAGGGGATCAGGTCATTTTTGCTGGTCCTGAAACCAATGTTGAAGGCTTAAATTCTGAATCTAACATGTCATTCGATAGATCTGTCACAGACAGTCACGGGGGTCCACTGGATAAACCAAGTGGACTGGGTTTTGGCTTCTCAGAGCAAAAGAATAAGAAATCTTCAGGTAGTGGGTTGAAAAGCTGCTGTGTGGTTCCACAGGCTGCTTTGAAACGAGTAACTGGCAAAGCTTTGCCGGGTTTCAGGTTCCTGAAAACCAACTTGCTTGATATGGATGTAGCACTGCCTGAAGAAGCTTTAAGACCATCGAAATCACATCCAAACCGTAGAAGAGCTTGGCGTGTATTTGTTAAATCGTCGCAAAGTATATACGAGTTGGTTCAGGCAACAATTGTGGTAGAAGATATGATTAAGACAGAGTACTTGAAAAATGAATGGTGGTACTGGTCTTCTCTTTCAGCGGCTGCTAAAATCTCGACTCTCTCAGCGTTATCCGTCCGTATCTTCTCCCTCGACGCTGCTATCATTTATGATAAACCCATAACTCCATCAAATCCTATCGATGAAACAAAGCCGATCATCAGCTTACCGGACCAAAAGTCACAGCCGGTTTCGGATTCTCAAGAAAGAAGCAGCAGAGTTAGAAGATCTGGCAAGAAAAGGAAAGAACCCGAGGGATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0001763 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004402 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005911 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008080 GO:0008150 GO:0008152 GO:0008327 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009648 GO:0009653 GO:0009791 GO:0009889 GO:0009987 GO:0010016 GO:0010216 GO:0010223 GO:0010228 GO:0010346 GO:0010468 GO:0010556 GO:0016043 GO:0016407 GO:0016410 GO:0016567 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022414 GO:0030054 GO:0031323 GO:0031326 GO:0031935 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0034212 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043565 GO:0043966 GO:0043967 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048367 GO:0048573 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051128 GO:0051171 GO:0051252 GO:0051276 GO:0055044 GO:0060255 GO:0060968 GO:0061458 GO:0061630 GO:0061659 GO:0061733 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0097159 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905393 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN MBD PHD WHIM1 WSD

Protein Analysis

2176

Amino Acids

240.43

Weight (kDa)

5.34

Isoelectric Point (pI)

49.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 86 - 130 1.8e-07 PHD-finger
MBD PF01429 259 - 372 2.3e-16 Methyl-CpG binding domain
FYRN PF05964 404 - 438 3.2e-06 F/Y-rich N-terminus
PHD PF00628 1290 - 1334 2.3e-11 PHD-finger
WHIM1 PF15612 1372 - 1413 3.7e-10 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 1627 - 1725 9.8e-06 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3541
AasI GACNNNNNNGTC 1 cut(s) 609
AatII GACGTC 1 cut(s) 506
Acc36I ACCTGC 1 cut(s) 3541
Acc65I GGTACC 1 cut(s) 1805
AccB1I GGYRCC 2 cut(s) 1805, 2060
AccB7I CCANNNNNTGG 6 cut(s) 212, 1040, 1100, 1571, 2159, 5837
AccI GTMKAC 1 cut(s) 3649
AccII CGCG 2 cut(s) 263, 3547
AcoI YGGCCR 2 cut(s) 419, 1031
AcvI CACGTG 2 cut(s) 1938, 2903
AcyI GRCGYC 1 cut(s) 503
AfeI AGCGCT 1 cut(s) 2139
AflII CTTAAG 3 cut(s) 3758, 4184, 4682
AflIII ACRYGT 4 cut(s) 1349, 1937, 3652, 5910
AhdI GACNNNNNGTC 1 cut(s) 6445
AhlI ACTAGT 2 cut(s) 4048, 4811
AjuI GAANNNNNNNTTGG 2 cut(s) 2707, 2739
AleI CACNNNNGTG 1 cut(s) 3226
Alw21I GWGCWC 5 cut(s) 291, 874, 1078, 2635, 5808
Alw44I GTGCAC 1 cut(s) 2631
AlwNI CAGNNNCTG 3 cut(s) 212, 387, 6311
Ama87I CYCGRG 3 cut(s) 1653, 4092, 6518
Aor51HI AGCGCT 1 cut(s) 2139
AoxI GGCC 7 cut(s) 419, 1031, 2267, 2825, 2835, 3082, 3846
ApaLI GTGCAC 1 cut(s) 2631
ArsI GACNNNNNNTTYG 4 cut(s) 493, 525, 2168, 2200
AseI ATTAAT 1 cut(s) 933
Asp700I GAANNNNTTC 4 cut(s) 1607, 2104, 3615, 3627
Asp718I GGTACC 1 cut(s) 1805
AspLEI GCGC 5 cut(s) 113, 263, 1635, 2140, 4017
AsuC2I CCSGG 4 cut(s) 200, 4536, 4580, 6084
AsuNHI GCTAGC 1 cut(s) 3319
AvaI CYCGRG 3 cut(s) 1653, 4092, 6518
AvaII GGWCC 9 cut(s) 202, 1376, 1690, 3290, 3685, 5294, 5870, 5948, 6439
BaeGI GKGCMC 1 cut(s) 2635
BalI TGGCCA 1 cut(s) 1033
BamHI GGATCC 2 cut(s) 742, 6524
BanI GGYRCC 2 cut(s) 1805, 2060
BanII GRGCYC 5 cut(s) 220, 291, 2650, 3136, 3522
BarI GAAGNNNNNNTAC 4 cut(s) 2089, 2121, 3822, 3854
BauI CACGAG 2 cut(s) 2613, 4458
BbrPI CACGTG 2 cut(s) 1938, 2903
BbsI GAAGAC 5 cut(s) 277, 1432, 5062, 5348, 6288
Bbv12I GWGCWC 5 cut(s) 291, 874, 1078, 2635, 5808
BbvCI CCTCAGC 4 cut(s) 480, 495, 3732, 4164
BceAI ACGGC 5 cut(s) 169, 406, 1109, 3402, 4347
BcgI CGANNNNNNTGC 2 cut(s) 3525, 3559
BciVI GTATCC 2 cut(s) 479, 1855
BclI TGATCA 2 cut(s) 1684, 5742
BcnI CCSGG 4 cut(s) 200, 4536, 4580, 6084
BcuI ACTAGT 2 cut(s) 4048, 4811
BfmI CTRYAG 7 cut(s) 282, 943, 2124, 2484, 3444, 3766, 4617
BfoI RGCGCY 2 cut(s) 114, 2141
BfrI CTTAAG 3 cut(s) 3758, 4184, 4682
BfuAI ACCTGC 1 cut(s) 3541
BfuI GTATCC 2 cut(s) 479, 1855
BglII AGATCT 3 cut(s) 5682, 5924, 6494
BmcAI AGTACT 2 cut(s) 1510, 6269
Bme18I GGWCC 9 cut(s) 202, 1376, 1690, 3290, 3685, 5294, 5870, 5948, 6439
BmeRI GACNNNNNGTC 1 cut(s) 6445
BmeT110I CYCGRG 3 cut(s) 1653, 4092, 6518
BmrI ACTGGG 5 cut(s) 465, 1867, 3485, 4159, 5981
BmtI GCTAGC 1 cut(s) 3323
BmuI ACTGGG 5 cut(s) 465, 1867, 3485, 4159, 5981
BoxI GACNNNNGTC 1 cut(s) 277
BpiI GAAGAC 5 cut(s) 277, 1432, 5062, 5348, 6288
BplI GAGNNNNNCTC 8 cut(s) 56, 88, 2272, 2304, 2354, 2386, 4460, 4492
BpmI CTGGAG 2 cut(s) 234, 5330
Bpu10I CCTNAGC 6 cut(s) 480, 495, 1050, 3135, 3732, 4164
BpuEI CTTGAG 7 cut(s) 571, 1497, 3653, 4008, 4256, 5276, 6453
BpuMI CCSGG 4 cut(s) 200, 4536, 4580, 6084
Bsa29I ATCGAT 2 cut(s) 87, 6408
BsaAI YACGTR 4 cut(s) 1598, 1938, 2903, 3653
BsaBI GATNNNNATC 3 cut(s) 733, 4947, 5133
BsaHI GRCGYC 1 cut(s) 503
BsaI GGTCTC 3 cut(s) 4667, 5142, 5252
BsaWI WCCGGW 3 cut(s) 238, 5236, 6436
BsaXI ACNNNNNCTCC 8 cut(s) 1099, 1129, 2127, 2157, 3330, 3360, 4437, 4467
Bse118I RCCGGY 3 cut(s) 107, 5582, 6454
Bse3DI GCAATG 5 cut(s) 573, 3286, 3314, 3543, 4005
Bse8I GATNNNNATC 3 cut(s) 733, 4947, 5133
BseCI ATCGAT 2 cut(s) 87, 6408
BseJI GATNNNNATC 3 cut(s) 733, 4947, 5133
BseMI GCAATG 5 cut(s) 573, 3286, 3314, 3543, 4005
BseRI GAGGAG 6 cut(s) 421, 512, 2247, 4181, 4623, 5693
BseSI GKGCMC 1 cut(s) 2635
BseYI CCCAGC 2 cut(s) 205, 2975
BsgI GTGCAG 5 cut(s) 400, 879, 2883, 3368, 3810
Bsh1236I CGCG 2 cut(s) 263, 3547
BshFI GGCC 7 cut(s) 421, 1033, 2269, 2827, 2837, 3084, 3848
BshNI GGYRCC 2 cut(s) 1805, 2060
BshVI ATCGAT 2 cut(s) 87, 6408
BsiHKAI GWGCWC 5 cut(s) 291, 874, 1078, 2635, 5808
BsiHKCI CYCGRG 3 cut(s) 1653, 4092, 6518
BslFI GGGAC 4 cut(s) 1362, 3190, 3534, 5006
BsmBI CGTCTC 3 cut(s) 494, 4440, 5187
BsmFI GGGAC 4 cut(s) 1362, 3190, 3534, 5006
BsmI GAATGC 5 cut(s) 574, 3250, 3785, 5387, 5757
BsnI GGCC 7 cut(s) 421, 1033, 2269, 2827, 2837, 3084, 3848
Bso31I GGTCTC 3 cut(s) 4667, 5142, 5252
BsoBI CYCGRG 3 cut(s) 1653, 4092, 6518
Bsp1286I GDGCHC 9 cut(s) 220, 291, 874, 1078, 2635, 2650, 3136, 3522, 5808
Bsp1407I TGTACA 1 cut(s) 2032
Bsp19I CCATGG 3 cut(s) 1693, 2865, 5050
BspANI GGCC 7 cut(s) 421, 1033, 2269, 2827, 2837, 3084, 3848
BspDI ATCGAT 2 cut(s) 87, 6408
BspFNI CGCG 2 cut(s) 263, 3547
BspHI TCATGA 3 cut(s) 4702, 4750, 5125
BspMAI CTGCAG 1 cut(s) 3448
BspMI ACCTGC 1 cut(s) 3541
BspOI GCTAGC 1 cut(s) 3323
BspQI GCTCTTC 1 cut(s) 6172
BspT107I GGYRCC 2 cut(s) 1805, 2060
BspTI CTTAAG 3 cut(s) 3758, 4184, 4682
BspTNI GGTCTC 3 cut(s) 4667, 5142, 5252
BsrDI GCAATG 5 cut(s) 573, 3286, 3314, 3543, 4005
BsrFI RCCGGY 3 cut(s) 107, 5582, 6454
BsrGI TGTACA 1 cut(s) 2032
BssAI RCCGGY 3 cut(s) 107, 5582, 6454
BssNAI GTATAC 1 cut(s) 3650
BssNI GRCGYC 1 cut(s) 503
BssSI CACGAG 2 cut(s) 2613, 4458
BssT1I CCWWGG 7 cut(s) 738, 1693, 2691, 2865, 3843, 5050, 5262
Bst1107I GTATAC 1 cut(s) 3650
Bst2BI CACGAG 2 cut(s) 2613, 4458
Bst6I CTCTTC 7 cut(s) 57, 987, 1598, 2087, 2355, 5279, 6172
BstACI GRCGYC 1 cut(s) 503
BstAFI CTTAAG 3 cut(s) 3758, 4184, 4682
BstAPI GCANNNNNTGC 2 cut(s) 540, 2894
BstAUI TGTACA 1 cut(s) 2032
BstBAI YACGTR 4 cut(s) 1598, 1938, 2903, 3653
BstC8I GCNNGC 8 cut(s) 528, 532, 815, 1319, 2960, 3321, 4785, 5584
BstDSI CCRYGG 4 cut(s) 1693, 2865, 3851, 5050
BstEII GGTNACC 1 cut(s) 1398
BstENI CCTNNNNNAGG 1 cut(s) 2387
BstFNI CGCG 2 cut(s) 263, 3547
BstH2I RGCGCY 2 cut(s) 114, 2141
BstHHI GCGC 5 cut(s) 113, 263, 1635, 2140, 4017
BstNSI RCATGY 7 cut(s) 608, 1353, 1782, 1832, 3924, 4550, 5914
BstPAI GACNNNNGTC 1 cut(s) 277
BstPI GGTNACC 1 cut(s) 1398
BstSFI CTRYAG 7 cut(s) 282, 943, 2124, 2484, 3444, 3766, 4617
BstSLI GKGCMC 1 cut(s) 2635
BstUI CGCG 2 cut(s) 263, 3547
BstV2I GAAGAC 5 cut(s) 277, 1432, 5062, 5348, 6288
BstX2I RGATCY 7 cut(s) 742, 1542, 2738, 5682, 5924, 6494, 6524
BstXI CCANNNNNNTGG 3 cut(s) 3228, 4018, 5278
BstYI RGATCY 7 cut(s) 742, 1542, 2738, 5682, 5924, 6494, 6524
BstZ17I GTATAC 1 cut(s) 3650
Bsu15I ATCGAT 2 cut(s) 87, 6408
BsuI GTATCC 2 cut(s) 479, 1855
BsuRI GGCC 7 cut(s) 421, 1033, 2269, 2827, 2837, 3084, 3848
BsuTUI ATCGAT 2 cut(s) 87, 6408
BtgI CCRYGG 4 cut(s) 1693, 2865, 3851, 5050
BtgZI GCGATG 3 cut(s) 318, 3939, 4212
BtsI GCAGTG 7 cut(s) 867, 1558, 1936, 3118, 4247, 5413, 6128
BveI ACCTGC 1 cut(s) 3541
Cac8I GCNNGC 8 cut(s) 528, 532, 815, 1319, 2960, 3321, 4785, 5584
CaiI CAGNNNCTG 3 cut(s) 212, 387, 6311
CciI TCATGA 3 cut(s) 4702, 4750, 5125
CfoI GCGC 5 cut(s) 113, 263, 1635, 2140, 4017
Cfr10I RCCGGY 3 cut(s) 107, 5582, 6454
ClaI ATCGAT 2 cut(s) 87, 6408
CseI GACGC 3 cut(s) 1538, 3536, 6371
CsiI ACCWGGT 1 cut(s) 3687
CspCI CAANNNNNGTGG 2 cut(s) 6222, 6257
DraI TTTAAA 2 cut(s) 840, 3951
DrdI GACNNNNNNGTC 1 cut(s) 609
DriI GACNNNNNGTC 1 cut(s) 6445
DseDI GACNNNNNNGTC 1 cut(s) 609
EaeI YGGCCR 2 cut(s) 419, 1031
Eam1104I CTCTTC 7 cut(s) 57, 987, 1598, 2087, 2355, 5279, 6172
Eam1105I GACNNNNNGTC 1 cut(s) 6445
EarI CTCTTC 7 cut(s) 57, 987, 1598, 2087, 2355, 5279, 6172
EciI GGCGGA 2 cut(s) 355, 4075
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 7 cut(s) 738, 1693, 2691, 2865, 3843, 5050, 5262
Eco24I GRGCYC 5 cut(s) 220, 291, 2650, 3136, 3522
Eco31I GGTCTC 3 cut(s) 4667, 5142, 5252
Eco32I GATATC 1 cut(s) 5642
Eco47I GGWCC 9 cut(s) 202, 1376, 1690, 3290, 3685, 5294, 5870, 5948, 6439
Eco47III AGCGCT 1 cut(s) 2139
Eco53kI GAGCTC 1 cut(s) 289
Eco72I CACGTG 2 cut(s) 1938, 2903
Eco88I CYCGRG 3 cut(s) 1653, 4092, 6518
Eco91I GGTNACC 1 cut(s) 1398
EcoICRI GAGCTC 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 2387
EcoO109I RGGNCCY 1 cut(s) 3847
EcoO65I GGTNACC 1 cut(s) 1398
EcoRI GAATTC 5 cut(s) 824, 976, 3493, 3969, 4322
EcoRV GATATC 1 cut(s) 5642
EcoT14I CCWWGG 7 cut(s) 738, 1693, 2691, 2865, 3843, 5050, 5262
EcoT22I ATGCAT 7 cut(s) 576, 664, 1747, 1780, 3286, 4759, 5303
EcoT38I GRGCYC 5 cut(s) 220, 291, 2650, 3136, 3522
ErhI CCWWGG 7 cut(s) 738, 1693, 2691, 2865, 3843, 5050, 5262
Esp3I CGTCTC 3 cut(s) 494, 4440, 5187
FaqI GGGAC 4 cut(s) 1362, 3190, 3534, 5006
FauNDI CATATG 5 cut(s) 327, 522, 2052, 2799, 5423
FbaI TGATCA 2 cut(s) 1684, 5742
FblI GTMKAC 1 cut(s) 3649
FriOI GRGCYC 5 cut(s) 220, 291, 2650, 3136, 3522
GlaI GCGC 5 cut(s) 112, 262, 1634, 2139, 4016
GsaI CCCAGC 2 cut(s) 209, 2979
GsuI CTGGAG 2 cut(s) 234, 5330
HaeII RGCGCY 2 cut(s) 114, 2141
HaeIII GGCC 7 cut(s) 421, 1033, 2269, 2827, 2837, 3084, 3848
HgaI GACGC 3 cut(s) 1538, 3536, 6371
HhaI GCGC 5 cut(s) 113, 263, 1635, 2140, 4017
Hin1I GRCGYC 1 cut(s) 503
Hin6I GCGC 5 cut(s) 111, 261, 1633, 2138, 4015
HinP1I GCGC 5 cut(s) 111, 261, 1633, 2138, 4015
HincII GTYRAC 6 cut(s) 340, 1285, 1834, 3036, 3886, 5047
HindII GTYRAC 6 cut(s) 340, 1285, 1834, 3036, 3886, 5047
HindIII AAGCTT 9 cut(s) 580, 618, 3158, 3204, 3612, 3836, 4187, 6075, 6141
Hpy99I CGWCG 3 cut(s) 155, 6208, 6365
HpyCH4IV ACGT 8 cut(s) 503, 1287, 1597, 1937, 2902, 3652, 5078, 5196
HpySE526I ACGT 8 cut(s) 503, 1287, 1597, 1937, 2902, 3652, 5078, 5196
Hsp92I GRCGYC 1 cut(s) 503
HspAI GCGC 5 cut(s) 111, 261, 1633, 2138, 4015
KpnI GGTACC 1 cut(s) 1809
KroI GCCGGC 1 cut(s) 5582
KroNI GCCGGC 1 cut(s) 5584
Ksp22I TGATCA 2 cut(s) 1684, 5742
LguI GCTCTTC 1 cut(s) 6172
MabI ACCWGGT 1 cut(s) 3687
MaeII ACGT 8 cut(s) 503, 1287, 1597, 1937, 2902, 3652, 5078, 5196
MfeI CAATTG 2 cut(s) 1785, 6237
MflI RGATCY 7 cut(s) 742, 1542, 2738, 5682, 5924, 6494, 6524
MhlI GDGCHC 9 cut(s) 220, 291, 874, 1078, 2635, 2650, 3136, 3522, 5808
MlsI TGGCCA 1 cut(s) 1033
MluNI TGGCCA 1 cut(s) 1033
MlyI GAGTC 7 cut(s) 76, 287, 2080, 4550, 5168, 5792, 6319
MmeI TCCRAC 4 cut(s) 3188, 4423, 4672, 5786
Mox20I TGGCCA 1 cut(s) 1033
Mph1103I ATGCAT 7 cut(s) 576, 664, 1747, 1780, 3286, 4759, 5303
MroNI GCCGGC 1 cut(s) 5582
MroXI GAANNNNTTC 4 cut(s) 1607, 2104, 3615, 3627
MscI TGGCCA 1 cut(s) 1033
MslI CAYNNNNRTG 5 cut(s) 1027, 1718, 3226, 5130, 5276
Msp20I TGGCCA 1 cut(s) 1033
MspA1I CMGCKG 6 cut(s) 53, 137, 948, 1427, 3443, 6308
MspCI CTTAAG 3 cut(s) 3758, 4184, 4682
MunI CAATTG 2 cut(s) 1785, 6237
Mva1269I GAATGC 5 cut(s) 574, 3250, 3785, 5387, 5757
MvnI CGCG 2 cut(s) 263, 3547
NaeI GCCGGC 1 cut(s) 5584
NciI CCSGG 4 cut(s) 200, 4536, 4580, 6084
NcoI CCATGG 3 cut(s) 1693, 2865, 5050
NdeI CATATG 5 cut(s) 327, 522, 2052, 2799, 5423
NgoMIV GCCGGC 1 cut(s) 5582
NheI GCTAGC 1 cut(s) 3319
NmeAIII GCCGAG 3 cut(s) 60, 99, 250
NsiI ATGCAT 7 cut(s) 576, 664, 1747, 1780, 3286, 4759, 5303
NspI RCATGY 7 cut(s) 608, 1353, 1782, 1832, 3924, 4550, 5914
OliI CACNNNNGTG 1 cut(s) 3226
PaeR7I CTCGAG 1 cut(s) 1653
PagI TCATGA 3 cut(s) 4702, 4750, 5125
PaqCI CACCTGC 1 cut(s) 3541
PasI CCCWGGG 1 cut(s) 4598
PciI ACATGT 2 cut(s) 1349, 5910
PciSI GCTCTTC 1 cut(s) 6172
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 260, 509
PctI GAATGC 5 cut(s) 574, 3250, 3785, 5387, 5757
PdiI GCCGGC 1 cut(s) 5584
PdmI GAANNNNTTC 4 cut(s) 1607, 2104, 3615, 3627
PflFI GACNNNGTC 1 cut(s) 1111
PflMI CCANNNNNTGG 6 cut(s) 212, 1040, 1100, 1571, 2159, 5837
PleI GAGTC 7 cut(s) 75, 286, 2080, 4549, 5167, 5792, 6319
PmaCI CACGTG 2 cut(s) 1938, 2903
PmlI CACGTG 2 cut(s) 1938, 2903
PpsI GAGTC 7 cut(s) 75, 286, 2080, 4549, 5167, 5792, 6319
Ppu21I YACGTR 4 cut(s) 1598, 1938, 2903, 3653
PscI ACATGT 2 cut(s) 1349, 5910
PshAI GACNNNNGTC 1 cut(s) 277
PshBI ATTAAT 1 cut(s) 933
Psp124BI GAGCTC 1 cut(s) 291
PspCI CACGTG 2 cut(s) 1938, 2903
PspEI GGTNACC 1 cut(s) 1398
PspFI CCCAGC 2 cut(s) 205, 2975
PsrI GAACNNNNNNTAC 2 cut(s) 3732, 3764
PstI CTGCAG 1 cut(s) 3448
PstNI CAGNNNCTG 3 cut(s) 212, 387, 6311
PsuI RGATCY 7 cut(s) 742, 1542, 2738, 5682, 5924, 6494, 6524
PsyI GACNNNGTC 1 cut(s) 1111
PvuII CAGCTG 3 cut(s) 948, 1427, 3443
RseI CAYNNNNRTG 5 cut(s) 1027, 1718, 3226, 5130, 5276
SacI GAGCTC 1 cut(s) 291
SapI GCTCTTC 1 cut(s) 6172
ScaI AGTACT 2 cut(s) 1510, 6269
SchI GAGTC 7 cut(s) 76, 287, 2080, 4550, 5168, 5792, 6319
SduI GDGCHC 9 cut(s) 220, 291, 874, 1078, 2635, 2650, 3136, 3522, 5808
SexAI ACCWGGT 1 cut(s) 3687
SfcI CTRYAG 7 cut(s) 282, 943, 2124, 2484, 3444, 3766, 4617
Sfr274I CTCGAG 1 cut(s) 1653
SinI GGWCC 9 cut(s) 202, 1376, 1690, 3290, 3685, 5294, 5870, 5948, 6439
SlaI CTCGAG 1 cut(s) 1653
SmiMI CAYNNNNRTG 5 cut(s) 1027, 1718, 3226, 5130, 5276
SpeI ACTAGT 2 cut(s) 4048, 4811
SstI GAGCTC 1 cut(s) 291
StyI CCWWGG 7 cut(s) 738, 1693, 2691, 2865, 3843, 5050, 5262
TaiI ACGT 8 cut(s) 506, 1290, 1600, 1940, 2905, 3655, 5081, 5199
TaqII GACCGA 1 cut(s) 5726
TatI WGTACW 5 cut(s) 1508, 1789, 2032, 2768, 6267
TauI GCSGC 9 cut(s) 53, 261, 359, 402, 469, 2958, 4060, 4318, 6311
TspGWI ACGGA 9 cut(s) 504, 2912, 3615, 4464, 4897, 5019, 5791, 6331, 6335
Tth111I GACNNNGTC 1 cut(s) 1111
Van91I CCANNNNNTGG 6 cut(s) 212, 1040, 1100, 1571, 2159, 5837
Vha464I CTTAAG 3 cut(s) 3758, 4184, 4682
VneI GTGCAC 1 cut(s) 2631
VpaK11BI GGWCC 9 cut(s) 202, 1376, 1690, 3290, 3685, 5294, 5870, 5948, 6439
VspI ATTAAT 1 cut(s) 933
XagI CCTNNNNNAGG 1 cut(s) 2387
XceI RCATGY 7 cut(s) 608, 1353, 1782, 1832, 3924, 4550, 5914
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XhoI CTCGAG 1 cut(s) 1653
XmiI GTMKAC 1 cut(s) 3649
XmnI GAANNNNTTC 4 cut(s) 1607, 2104, 3615, 3627
ZraI GACGTC 1 cut(s) 504
ZrmI AGTACT 2 cut(s) 1510, 6269
Zsp2I ATGCAT 7 cut(s) 576, 664, 1747, 1780, 3286, 4759, 5303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.