pycom111g02810

methyl-CpG-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0004967
Physical Location & Seq
Reverse (-)
1683650 .. 1688294
4645 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02810.3

Sequence Viewer

Length: 2739 bp
ATGGAACTGACGGATTCAGCTGCCGACGAACTCAGAGCAGCGGCAGCCGCCGCCGCCAACGCCGAGCCAACTCGCTCCGTACTCTGCATCGATCTCAATGAGATCCCTTCTCCTTCCGAAACCCTACCCGATTCCTTCGACGTTGTCCGGTCTTACCACGACCACCCTTCGCCGCCTCCCGGCGGCCCGGCCGGAGTTCCCGGCTCAGCTCGAGGATCCGCATGCGCCGCCTGCGGCAAGCCCGAGGTTCGCGGGCACGTGGTGGTCTGCGACGGCTGCGAGCGCGGGTTTCACCTCACATGCGCCGGAATGCGCGGCCGGCAGGCGGTGAATTTGGTGGAGTGGGTCTGCGGGGACTGTTTGAGCGGTGGGGTTAGGAGCAAGCGGTGGCCGCTTGGAGTTAAGGCCAAGCAGATTCTCGACATCAACGCCTCGCCGCCGAGCGACGTCGACGACATCGCGGAAATGCGCGAATTGAGAAAGCACACTCCCGGTGGTAATTCTTTTGGTGGCAATCCATTTGGTGCCCCAGTGACATATTCAAACTTCTTGTACTCTGGAAACGGATATGGCTTGGAAAAAGGTGCTGGGATTACACATGCGACTAAAGTGGGTTGGGAAGATATATTGCACCACACACAGACTATGGGTGGAAGCTTTGAGGAATTAAGTTCGAGGTTTCCGCTCGGGAAGCATAGTAATAACAATAATACGGCTATTAGAATACCATCCCGGAGTCCAGATGAGATAGTTCTACAGGCTCTTAGAGATTACGTTTCTGAAAGGCATGGTGTACTGGAGGATGGTTGGCGTGTAGAATTTAAACAGTCAACAGCCAGTGGTGAACCATATTTAGTATACTGTGCACCAAATGGGAAGACATTTGGCTCAGTATCTGAAGTTGCATATTTTCTTGGCTTGACGCCTAACTACAATTCCATGGGATCTGAAATAAGATGGGAGGGTTCTCTTTCTAATACTGAGAAGACTTATCTACCTAGAAAAAGAAAGTCAAGACTCTTATATTCCAACGGAGTAGCTGAAAATAAGGAAAGTTTGTTCAGTGGCTATTGCAAGGAGCTCTCTTCCAATGGTATAAGTATGGAAGCTTTTGCCCGTGGTTTTGGAAATAATGTAAAACTTACTGAAGCAGGGACAGAAGAACATGGTTGCCTTGGGTCTCGGCAAAGTACTGAGGGATTTCCTGTGCAGTTTGAAGATTTCTTTGTTCTTTCTTTGGGAGAAGTTGATACAAGGCCTTCTTATCATGATTCAAATCAGATCTATCCTGTAGGTTATAGGTCATGTTGGCATGATAAGGTTACTGGTTCTTTATTTGTGTGTGAAGTTTTGAATGGTGGTGATTCTGGACCTGTTTTTAAGGTCAGAAGATGTTCGTGTTCTGCCTTGCCAGTTCCCAAGGGTTCAACTATCCTCTGTAGGCCACAACTTGGCAACTTTTACAGTCAAATTAATCAAGAAAGTCATGACTTGACTTCTGATAATGATGGAAGTATCCAGATGATACTGTCAGATCCTTCTCCGCCTATGGAAAATGATATTTTGTCTTGCCTGAGGAGTTGTTCGGTTGAAGTCAGTGATGTCCAGAAATCAGCTGAACCGCAGTTTGAAGATAATTCCGTTTATGAAAAATCTGGAACTCTTTCATCTGCTGATTTGGGTGTGATGGATGACATTGGTGAGATTTCAGTAGAAGACCATTCTTCATCTGCAGCTTGGGGAATGATTTCAAAAAAAATTGTTAATGCTTGTTCTGAAATATTTAAACAGAAGGGCATTCTTAAGGTTTTCTGTAAGCATGTTGAAAATGCTCAAGGCTCCCAAAATGAGGTTATAACAAATGACAGTGGCAACGTGAACCAATCTCCACTGGACAAGTTTTGTAGTTCACCAGGTTCCGTCAGCATCTCTTTTGAACTTCAGGCCGATGATGATCGTGGCTTTTCTTATGACGTACTAGCGAATTGGCTAGACCAGGACAGATTTGGATTAGATGTGGATTTTGTGCAAGAACTTTTGGAGCAGCTTCCTGCTGCCCAATCCTGTTCTCAATATCAATTTCTTAACGGTAGAAGTTCTAATTCTACACAGCTAACTGTCGGAAATGGGCTTCTAGTGGTTAAAATGGGAGCTGGATTACATGGTAAAGAAGAAGTGTTGGATGGTTTGTTTAGGAGGTCCAAAAAAGTGAAGTTGGTCAAGGATCACCTCAAGATTGATCATCCACCTCCTCATGGGAAGCCATTGTGCTTGAGGATTCCTCCTGCCCTTGTTGGTGATGTTTATCAGGTTTGGGAATTATTATCGCACTTTGATGAAATTTTGGGTCTAAAGGAGGCTTTTTCGTTGGAAGAACTTGAGGAGGAACTTATTGACCCTTGGTTCGGCAGGTCAGATCTTTCAGAGAAGTTTGATAGGGAAAACCAGGGCACTCAAGCTTTAAATTCAGATAGAATTGATTATTCAAGTGGTCAATTATCGTTGACCATGGAGTCTGGCTTGGCAGTTTCTAGGAATAATCCACATGCATTCATACATATGGAAACTGGAGCAATGAAGGAGGCAGCTCAAGATAAACTTGCATCTGTAACTTATAGCAGATGTTCTGGCATAGCTTTGACGAAGGCTCATGCATCACTACTAAGAGTGCTCATAGGTGAGTTGCAGTTTAAGGTTGCTGCCCTGGTGGATCCGAATTTTGATTCTGGATCGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0001763 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004402 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005911 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008080 GO:0008150 GO:0008152 GO:0008327 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009648 GO:0009653 GO:0009791 GO:0009889 GO:0009987 GO:0010016 GO:0010216 GO:0010223 GO:0010228 GO:0010346 GO:0010468 GO:0010556 GO:0016043 GO:0016407 GO:0016410 GO:0016567 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022414 GO:0030054 GO:0031323 GO:0031326 GO:0031935 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0034212 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043565 GO:0043966 GO:0043967 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048367 GO:0048573 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051128 GO:0051171 GO:0051252 GO:0051276 GO:0055044 GO:0060255 GO:0060968 GO:0061458 GO:0061630 GO:0061659 GO:0061733 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0097159 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905393 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromodomain DDT FYRC MBD PHD WHIM1 WSD

Protein Analysis

913

Amino Acids

99.21

Weight (kDa)

5.17

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1856
AasI GACNNNNNNGTC 1 cut(s) 2512
AatII GACGTC 1 cut(s) 450
Acc36I ACCTGC 1 cut(s) 2400
AccB1I GGYRCC 1 cut(s) 524
AccB7I CCANNNNNTGG 1 cut(s) 1451
AccBSI CCGCTC 2 cut(s) 366, 685
AccI GTMKAC 2 cut(s) 450, 858
AccII CGCG 5 cut(s) 252, 285, 315, 461, 471
AclWI GGATC 9 cut(s) 97, 210, 223, 952, 1529, 2232, 2705, 2718, 2737
AcoI YGGCCR 3 cut(s) 189, 316, 389
AcsI RAATTY 5 cut(s) 331, 818, 2340, 2464, 2716
AcuI CTGAAG 3 cut(s) 918, 1167, 1925
AcvI CACGTG 1 cut(s) 259
AcyI GRCGYC 2 cut(s) 447, 923
AdeI CACNNNGTG 1 cut(s) 262
AfaI GTAC 5 cut(s) 81, 554, 795, 1192, 1977
AfiI CCNNNNNNNGG 7 cut(s) 179, 182, 325, 1451, 1849, 2255, 2405
AflII CTTAAG 1 cut(s) 1802
AjnI CCWGG 4 cut(s) 1912, 1995, 2445, 2703
AloI GAACNNNNNNTCC 2 cut(s) 1043, 1075
Alw21I GWGCWC 3 cut(s) 868, 1083, 2673
Alw26I GTCTC 1 cut(s) 1185
Alw44I GTGCAC 1 cut(s) 864
AlwI GGATC 9 cut(s) 97, 210, 223, 952, 1529, 2232, 2705, 2718, 2737
AlwNI CAGNNNCTG 1 cut(s) 896
Ama87I CYCGRG 3 cut(s) 210, 242, 686
AoxI GGCC 8 cut(s) 184, 189, 316, 389, 405, 1256, 1442, 1944
ApaLI GTGCAC 1 cut(s) 864
ApeKI GCWGC 9 cut(s) 20, 38, 44, 276, 1733, 2044, 2054, 2585, 2699
ApoI RAATTY 5 cut(s) 331, 818, 2340, 2464, 2716
ArsI GACNNNNNNTTYG 2 cut(s) 2387, 2419
AseI ATTAAT 1 cut(s) 1473
Asp700I GAANNNNTTC 3 cut(s) 1393, 1663, 1747
AspLEI GCGC 5 cut(s) 227, 285, 305, 315, 471
AspS9I GGNCC 3 cut(s) 185, 1370, 2199
AsuC2I CCSGG 5 cut(s) 180, 188, 201, 492, 733
AsuHPI GGTGA 9 cut(s) 284, 340, 854, 1373, 1712, 1902, 2219, 2309, 2690
AvaI CYCGRG 3 cut(s) 210, 242, 686
AvaII GGWCC 2 cut(s) 1370, 2199
AxyI CCTNAGG 1 cut(s) 1574
BaeGI GKGCMC 4 cut(s) 258, 529, 868, 2453
BamHI GGATCC 2 cut(s) 215, 2710
BanI GGYRCC 1 cut(s) 524
BanII GRGCYC 1 cut(s) 1083
BbrPI CACGTG 1 cut(s) 259
BbsI GAAGAC 3 cut(s) 884, 992, 1722
Bbv12I GWGCWC 3 cut(s) 868, 1083, 2673
BbvI GCAGC 9 cut(s) 7, 50, 56, 263, 1745, 2041, 2056, 2597, 2686
BccI CCATC 6 cut(s) 736, 797, 951, 1502, 1681, 2177
BceAI ACGGC 2 cut(s) 289, 729
BciT130I CCWGG 4 cut(s) 1914, 1997, 2447, 2705
BciVI GTATCC 1 cut(s) 1526
BclI TGATCA 1 cut(s) 2239
BcnI CCSGG 5 cut(s) 180, 188, 201, 492, 733
BcoDI GTCTC 1 cut(s) 1185
BfaI CTAG 5 cut(s) 999, 1979, 1991, 2135, 2532
BfmI CTRYAG 4 cut(s) 755, 1290, 1438, 1731
BfrI CTTAAG 1 cut(s) 1802
BfuAI ACCTGC 1 cut(s) 2400
BfuI GTATCC 1 cut(s) 1526
BglII AGATCT 2 cut(s) 1281, 2416
BlpI GCTNAGC 1 cut(s) 205
BmcAI AGTACT 1 cut(s) 1192
Bme1390I CCNGG 9 cut(s) 180, 188, 201, 492, 733, 1914, 1997, 2447, 2705
Bme18I GGWCC 2 cut(s) 1370, 2199
BmeT110I CYCGRG 3 cut(s) 210, 242, 686
BmgT120I GGNCC 3 cut(s) 185, 1370, 2199
BmiI GGNNCC 5 cut(s) 217, 526, 1840, 1918, 2712
BmrFI CCNGG 9 cut(s) 180, 188, 201, 492, 733, 1914, 1997, 2447, 2705
BmrI ACTGGG 1 cut(s) 524
BmsI GCATC 4 cut(s) 96, 1935, 2612, 2663
BmuI ACTGGG 1 cut(s) 524
BpiI GAAGAC 3 cut(s) 884, 992, 1722
BplI GAGNNNNNCTC 2 cut(s) 2266, 2298
BpmI CTGGAG 2 cut(s) 818, 2589
Bpu1102I GCTNAGC 1 cut(s) 205
BpuEI CTTGAG 6 cut(s) 1818, 2216, 2293, 2399, 2439, 2574
BpuMI CCSGG 5 cut(s) 180, 188, 201, 492, 733
Bsa29I ATCGAT 2 cut(s) 90, 2732
BsaAI YACGTR 1 cut(s) 259
BsaBI GATNNNNATC 3 cut(s) 1275, 1953, 2304
BsaHI GRCGYC 2 cut(s) 447, 923
BsaI GGTCTC 1 cut(s) 1185
BsaJI CCNNGG 9 cut(s) 243, 939, 1117, 1174, 1419, 2399, 2446, 2508, 2703
BsaWI WCCGGW 1 cut(s) 147
Bsc4I CCNNNNNNNGG 7 cut(s) 179, 182, 325, 1451, 1849, 2255, 2405
Bse118I RCCGGY 1 cut(s) 318
Bse1I ACTGG 7 cut(s) 530, 801, 837, 1330, 1412, 1896, 2572
Bse21I CCTNAGG 1 cut(s) 1574
Bse3DI GCAATG 1 cut(s) 2580
Bse8I GATNNNNATC 3 cut(s) 1275, 1953, 2304
BseBI CCWGG 4 cut(s) 1914, 1997, 2447, 2705
BseCI ATCGAT 2 cut(s) 90, 2732
BseDI CCNNGG 9 cut(s) 243, 939, 1117, 1174, 1419, 2399, 2446, 2508, 2703
BseGI GGATG 5 cut(s) 728, 808, 1696, 2188, 2242
BseJI GATNNNNATC 3 cut(s) 1275, 1953, 2304
BseLI CCNNNNNNNGG 7 cut(s) 179, 182, 325, 1451, 1849, 2255, 2405
BseMI GCAATG 1 cut(s) 2580
BseMII CTCAG 6 cut(s) 46, 219, 903, 972, 1185, 1565
BseNI ACTGG 7 cut(s) 530, 801, 837, 1330, 1412, 1896, 2572
BseRI GAGGAG 3 cut(s) 1591, 2241, 2396
BseSI GKGCMC 4 cut(s) 258, 529, 868, 2453
BseX3I CGGCCG 2 cut(s) 189, 316
BseXI GCAGC 9 cut(s) 7, 50, 56, 263, 1745, 2041, 2056, 2597, 2686
BseYI CCCAGC 1 cut(s) 587
BsgI GTGCAG 1 cut(s) 1229
Bsh1236I CGCG 5 cut(s) 252, 285, 315, 461, 471
Bsh1285I CGRYCG 2 cut(s) 192, 319
BshFI GGCC 8 cut(s) 186, 191, 318, 391, 407, 1258, 1444, 1946
BshNI GGYRCC 1 cut(s) 524
BshVI ATCGAT 2 cut(s) 90, 2732
BsiEI CGRYCG 2 cut(s) 192, 319
BsiHKAI GWGCWC 3 cut(s) 868, 1083, 2673
BsiHKCI CYCGRG 3 cut(s) 210, 242, 686
BsiSI CCGG 9 cut(s) 148, 180, 188, 192, 201, 306, 319, 492, 733
BslFI GGGAC 2 cut(s) 368, 1168
BslI CCNNNNNNNGG 7 cut(s) 179, 182, 325, 1451, 1849, 2255, 2405
BsmAI GTCTC 1 cut(s) 1185
BsmFI GGGAC 2 cut(s) 368, 1168
BsmI GAATGC 3 cut(s) 315, 1797, 2549
BsnI GGCC 8 cut(s) 186, 191, 318, 391, 407, 1258, 1444, 1946
Bso31I GGTCTC 1 cut(s) 1185
BsoBI CYCGRG 3 cut(s) 210, 242, 686
Bsp1286I GDGCHC 6 cut(s) 258, 529, 868, 1083, 2453, 2673
Bsp1720I GCTNAGC 1 cut(s) 205
Bsp19I CCATGG 2 cut(s) 939, 2508
BspANI GGCC 8 cut(s) 186, 191, 318, 391, 407, 1258, 1444, 1946
BspCNI CTCAG 6 cut(s) 45, 218, 902, 973, 1186, 1566
BspDI ATCGAT 2 cut(s) 90, 2732
BspFNI CGCG 5 cut(s) 252, 285, 315, 461, 471
BspHI TCATGA 2 cut(s) 1267, 1486
BspLI GGNNCC 5 cut(s) 217, 526, 1840, 1918, 2712
BspMAI CTGCAG 1 cut(s) 1735
BspMI ACCTGC 1 cut(s) 2400
BspPI GGATC 9 cut(s) 97, 210, 223, 952, 1529, 2232, 2705, 2718, 2737
BspT107I GGYRCC 1 cut(s) 524
BspTI CTTAAG 1 cut(s) 1802
BspTNI GGTCTC 1 cut(s) 1185
BsrBI CCGCTC 2 cut(s) 366, 685
BsrDI GCAATG 1 cut(s) 2580
BsrFI RCCGGY 1 cut(s) 318
BsrI ACTGG 7 cut(s) 530, 801, 837, 1330, 1412, 1896, 2572
BssAI RCCGGY 1 cut(s) 318
BssECI CCNNGG 9 cut(s) 243, 939, 1117, 1174, 1419, 2399, 2446, 2508, 2703
BssNAI GTATAC 1 cut(s) 859
BssNI GRCGYC 2 cut(s) 447, 923
BssT1I CCWWGG 5 cut(s) 939, 1174, 1419, 2399, 2508
Bst1107I GTATAC 1 cut(s) 859
Bst2UI CCWGG 4 cut(s) 1914, 1997, 2447, 2705
Bst4CI ACNGT 8 cut(s) 359, 828, 863, 1466, 1530, 1868, 2090, 2119
Bst6I CTCTTC 1 cut(s) 1090
BstACI GRCGYC 2 cut(s) 447, 923
BstAFI CTTAAG 1 cut(s) 1802
BstBAI YACGTR 1 cut(s) 259
BstC8I GCNNGC 8 cut(s) 223, 232, 239, 254, 281, 320, 324, 383
BstDEI CTNAG 8 cut(s) 32, 205, 764, 889, 981, 1194, 1574, 2663
BstDSI CCRYGG 3 cut(s) 939, 1117, 2508
BstF5I GGATG 5 cut(s) 728, 808, 1696, 2188, 2242
BstFNI CGCG 5 cut(s) 252, 285, 315, 461, 471
BstHHI GCGC 5 cut(s) 227, 285, 305, 315, 471
BstMAI GTCTC 1 cut(s) 1185
BstMCI CGRYCG 2 cut(s) 192, 319
BstNI CCWGG 4 cut(s) 1914, 1997, 2447, 2705
BstNSI RCATGY 5 cut(s) 225, 303, 602, 1823, 2549
BstSCI CCNGG 9 cut(s) 178, 186, 199, 490, 731, 1912, 1995, 2445, 2703
BstSFI CTRYAG 4 cut(s) 755, 1290, 1438, 1731
BstSLI GKGCMC 4 cut(s) 258, 529, 868, 2453
BstUI CGCG 5 cut(s) 252, 285, 315, 461, 471
BstV1I GCAGC 9 cut(s) 7, 50, 56, 263, 1745, 2041, 2056, 2597, 2686
BstV2I GAAGAC 3 cut(s) 884, 992, 1722
BstX2I RGATCY 7 cut(s) 102, 215, 944, 1281, 1534, 2416, 2710
BstYI RGATCY 7 cut(s) 102, 215, 944, 1281, 1534, 2416, 2710
BstZ17I GTATAC 1 cut(s) 859
BstZI CGGCCG 2 cut(s) 189, 316
Bsu15I ATCGAT 2 cut(s) 90, 2732
Bsu36I CCTNAGG 1 cut(s) 1574
BsuI GTATCC 1 cut(s) 1526
BsuRI GGCC 8 cut(s) 186, 191, 318, 391, 407, 1258, 1444, 1946
BsuTUI ATCGAT 2 cut(s) 90, 2732
BtgI CCRYGG 3 cut(s) 939, 1117, 2508
BtgZI GCGATG 1 cut(s) 442
BtsCI GGATG 5 cut(s) 728, 808, 1696, 2188, 2242
BtsIMutI CAGTG 6 cut(s) 537, 844, 1069, 1603, 1873, 1889
BveI ACCTGC 1 cut(s) 2400
Cac8I GCNNGC 8 cut(s) 223, 232, 239, 254, 281, 320, 324, 383
CaiI CAGNNNCTG 1 cut(s) 896
CciI TCATGA 2 cut(s) 1267, 1486
CfoI GCGC 5 cut(s) 227, 285, 305, 315, 471
Cfr10I RCCGGY 1 cut(s) 318
Cfr13I GGNCC 3 cut(s) 185, 1370, 2199
ClaI ATCGAT 2 cut(s) 90, 2732
CseI GACGC 1 cut(s) 931
CsiI ACCWGGT 1 cut(s) 1912
Csp6I GTAC 5 cut(s) 80, 553, 794, 1191, 1976
CviQI GTAC 5 cut(s) 80, 553, 794, 1191, 1976
DdeI CTNAG 8 cut(s) 32, 205, 764, 889, 981, 1194, 1574, 2663
DraI TTTAAA 3 cut(s) 823, 1786, 2463
DraIII CACNNNGTG 1 cut(s) 262
DrdI GACNNNNNNGTC 1 cut(s) 2512
DseDI GACNNNNNNGTC 1 cut(s) 2512
EaeI YGGCCR 3 cut(s) 189, 316, 389
EagI CGGCCG 2 cut(s) 189, 316
Eam1104I CTCTTC 1 cut(s) 1090
EarI CTCTTC 1 cut(s) 1090
EciI GGCGGA 1 cut(s) 1533
Ecl136II GAGCTC 1 cut(s) 1081
EclXI CGGCCG 2 cut(s) 189, 316
Eco130I CCWWGG 5 cut(s) 939, 1174, 1419, 2399, 2508
Eco147I AGGCCT 1 cut(s) 1258
Eco24I GRGCYC 1 cut(s) 1083
Eco31I GGTCTC 1 cut(s) 1185
Eco47I GGWCC 2 cut(s) 1370, 2199
Eco52I CGGCCG 2 cut(s) 189, 316
Eco53kI GAGCTC 1 cut(s) 1081
Eco57I CTGAAG 3 cut(s) 918, 1167, 1925
Eco72I CACGTG 1 cut(s) 259
Eco81I CCTNAGG 1 cut(s) 1574
Eco88I CYCGRG 3 cut(s) 210, 242, 686
EcoICRI GAGCTC 1 cut(s) 1081
EcoRII CCWGG 4 cut(s) 1912, 1995, 2445, 2703
EcoT14I CCWWGG 5 cut(s) 939, 1174, 1419, 2399, 2508
EcoT22I ATGCAT 2 cut(s) 2551, 2656
EcoT38I GRGCYC 1 cut(s) 1083
ErhI CCWWGG 5 cut(s) 939, 1174, 1419, 2399, 2508
FalI AAGNNNNNCTT 4 cut(s) 1246, 1278, 2583, 2615
FaqI GGGAC 2 cut(s) 368, 1168
FauI CCCGC 3 cut(s) 245, 278, 344
FauNDI CATATG 1 cut(s) 2559
FbaI TGATCA 1 cut(s) 2239
FblI GTMKAC 2 cut(s) 450, 858
FokI GGATG 5 cut(s) 715, 815, 1703, 2195, 2229
FriOI GRGCYC 1 cut(s) 1083
FspBI CTAG 5 cut(s) 999, 1979, 1991, 2135, 2532
GlaI GCGC 5 cut(s) 226, 284, 304, 314, 470
GsaI CCCAGC 1 cut(s) 591
GsuI CTGGAG 2 cut(s) 818, 2589
HaeIII GGCC 8 cut(s) 186, 191, 318, 391, 407, 1258, 1444, 1946
HapII CCGG 9 cut(s) 148, 180, 188, 192, 201, 306, 319, 492, 733
HgaI GACGC 1 cut(s) 931
HhaI GCGC 5 cut(s) 227, 285, 305, 315, 471
Hin1I GRCGYC 2 cut(s) 447, 923
Hin6I GCGC 5 cut(s) 225, 283, 303, 313, 469
HinP1I GCGC 5 cut(s) 225, 283, 303, 313, 469
HincII GTYRAC 3 cut(s) 451, 831, 2505
HindII GTYRAC 3 cut(s) 451, 831, 2505
HindIII AAGCTT 3 cut(s) 655, 1107, 2457
HpaII CCGG 9 cut(s) 148, 180, 188, 192, 201, 306, 319, 492, 733
HphI GGTGA 9 cut(s) 284, 340, 854, 1373, 1712, 1902, 2219, 2309, 2690
Hpy166II GTNNAC 9 cut(s) 451, 794, 831, 845, 859, 866, 1879, 1910, 2505
Hpy8I GTNNAC 9 cut(s) 451, 794, 831, 845, 859, 866, 1879, 1910, 2505
Hpy99I CGWCG 6 cut(s) 29, 143, 275, 449, 452, 455
HpyAV CCTTC 9 cut(s) 117, 123, 145, 177, 1269, 1548, 1786, 2572, 2638
HpyCH4III ACNGT 8 cut(s) 359, 828, 863, 1466, 1530, 1868, 2090, 2119
HpyCH4IV ACGT 6 cut(s) 141, 258, 447, 774, 1875, 1974
HpyF3I CTNAG 8 cut(s) 32, 205, 764, 889, 981, 1194, 1574, 2663
HpySE526I ACGT 6 cut(s) 141, 258, 447, 774, 1875, 1974
Hsp92I GRCGYC 2 cut(s) 447, 923
HspAI GCGC 5 cut(s) 225, 283, 303, 313, 469
KroI GCCGGC 1 cut(s) 318
KroNI GCCGGC 1 cut(s) 320
Ksp22I TGATCA 1 cut(s) 2239
LmnI GCTCC 7 cut(s) 80, 378, 1078, 1844, 2041, 2150, 2570
Lsp1109I GCAGC 9 cut(s) 7, 50, 56, 263, 1745, 2041, 2056, 2597, 2686
LweI GCATC 4 cut(s) 96, 1935, 2612, 2663
MabI ACCWGGT 1 cut(s) 1912
MaeI CTAG 5 cut(s) 999, 1979, 1991, 2135, 2532
MaeII ACGT 6 cut(s) 141, 258, 447, 774, 1875, 1974
MaeIII GTNAC 3 cut(s) 532, 1321, 2608
MbiI CCGCTC 2 cut(s) 366, 685
MflI RGATCY 7 cut(s) 102, 215, 944, 1281, 1534, 2416, 2710
MhlI GDGCHC 6 cut(s) 258, 529, 868, 1083, 2453, 2673
MlyI GAGTC 3 cut(s) 745, 1011, 2522
MmeI TCCRAC 4 cut(s) 1053, 2101, 2160, 2349
Mph1103I ATGCAT 2 cut(s) 2551, 2656
MroNI GCCGGC 1 cut(s) 318
MroXI GAANNNNTTC 3 cut(s) 1393, 1663, 1747
MslI CAYNNNNRTG 2 cut(s) 2334, 2558
MspA1I CMGCKG 3 cut(s) 20, 41, 1616
MspCI CTTAAG 1 cut(s) 1802
MspI CCGG 9 cut(s) 148, 180, 188, 192, 201, 306, 319, 492, 733
MspR9I CCNGG 9 cut(s) 180, 188, 201, 492, 733, 1914, 1997, 2447, 2705
Mva1269I GAATGC 3 cut(s) 315, 1797, 2549
MvaI CCWGG 4 cut(s) 1914, 1997, 2447, 2705
MvnI CGCG 5 cut(s) 252, 285, 315, 461, 471
NaeI GCCGGC 1 cut(s) 320
NciI CCSGG 5 cut(s) 180, 188, 201, 492, 733
NcoI CCATGG 2 cut(s) 939, 2508
NdeI CATATG 1 cut(s) 2559
NgoMIV GCCGGC 1 cut(s) 318
NlaIV GGNNCC 5 cut(s) 217, 526, 1840, 1918, 2712
NmeAIII GCCGAG 3 cut(s) 88, 465, 1162
NmuCI GTSAC 1 cut(s) 532
NsiI ATGCAT 2 cut(s) 2551, 2656
NspI RCATGY 5 cut(s) 225, 303, 602, 1823, 2549
PaeI GCATGC 1 cut(s) 225
PaeR7I CTCGAG 1 cut(s) 210
PagI TCATGA 2 cut(s) 1267, 1486
PceI AGGCCT 1 cut(s) 1258
PctI GAATGC 3 cut(s) 315, 1797, 2549
PdiI GCCGGC 1 cut(s) 320
PdmI GAANNNNTTC 3 cut(s) 1393, 1663, 1747
PfeI GAWTC 7 cut(s) 14, 131, 415, 1271, 1364, 2278, 2723
PflFI GACNNNGTC 1 cut(s) 143
PflMI CCANNNNNTGG 1 cut(s) 1451
PfoI TCCNGGA 1 cut(s) 731
PleI GAGTC 3 cut(s) 744, 1011, 2521
PmaCI CACGTG 1 cut(s) 259
PmlI CACGTG 1 cut(s) 259
PpsI GAGTC 3 cut(s) 744, 1011, 2521
Ppu21I YACGTR 1 cut(s) 259
PshBI ATTAAT 1 cut(s) 1473
PsiI TTATAA 1 cut(s) 1856
Psp124BI GAGCTC 1 cut(s) 1083
Psp6I CCWGG 4 cut(s) 1912, 1995, 2445, 2703
PspCI CACGTG 1 cut(s) 259
PspFI CCCAGC 1 cut(s) 587
PspGI CCWGG 4 cut(s) 1912, 1995, 2445, 2703
PspN4I GGNNCC 5 cut(s) 217, 526, 1840, 1918, 2712
PspPI GGNCC 3 cut(s) 185, 1370, 2199
PspXI VCTCGAGB 1 cut(s) 210
PstI CTGCAG 1 cut(s) 1735
PstNI CAGNNNCTG 1 cut(s) 896
PsuI RGATCY 7 cut(s) 102, 215, 944, 1281, 1534, 2416, 2710
PsyI GACNNNGTC 1 cut(s) 143
PvuII CAGCTG 2 cut(s) 20, 1616
RsaI GTAC 5 cut(s) 81, 554, 795, 1192, 1977
RsaNI GTAC 5 cut(s) 80, 553, 794, 1191, 1976
RseI CAYNNNNRTG 2 cut(s) 2334, 2558
SacI GAGCTC 1 cut(s) 1083
SalI GTCGAC 1 cut(s) 449
Sau96I GGNCC 3 cut(s) 185, 1370, 2199
ScaI AGTACT 1 cut(s) 1192
SchI GAGTC 3 cut(s) 745, 1011, 2522
ScrFI CCNGG 9 cut(s) 180, 188, 201, 492, 733, 1914, 1997, 2447, 2705
SduI GDGCHC 6 cut(s) 258, 529, 868, 1083, 2453, 2673
SexAI ACCWGGT 1 cut(s) 1912
SfaNI GCATC 4 cut(s) 96, 1935, 2612, 2663
SfcI CTRYAG 4 cut(s) 755, 1290, 1438, 1731
Sfr274I CTCGAG 1 cut(s) 210
SgrDI CGTCGACG 1 cut(s) 449
SinI GGWCC 2 cut(s) 1370, 2199
SlaI CTCGAG 1 cut(s) 210
SmiMI CAYNNNNRTG 2 cut(s) 2334, 2558
SmlI CTYRAG 8 cut(s) 210, 1802, 1833, 2231, 2272, 2378, 2454, 2589
SmoI CTYRAG 8 cut(s) 210, 1802, 1833, 2231, 2272, 2378, 2454, 2589
SphI GCATGC 1 cut(s) 225
SseBI AGGCCT 1 cut(s) 1258
SspI AATATT 1 cut(s) 1782
SspMI CTAG 5 cut(s) 999, 1979, 1991, 2135, 2532
SstI GAGCTC 1 cut(s) 1083
StuI AGGCCT 1 cut(s) 1258
StyD4I CCNGG 9 cut(s) 178, 186, 199, 490, 731, 1912, 1995, 2445, 2703
StyI CCWWGG 5 cut(s) 939, 1174, 1419, 2399, 2508
TaaI ACNGT 8 cut(s) 359, 828, 863, 1466, 1530, 1868, 2090, 2119
TaiI ACGT 6 cut(s) 144, 261, 450, 777, 1878, 1977
TaqI TCGA 7 cut(s) 90, 138, 211, 420, 450, 674, 2732
TatI WGTACW 3 cut(s) 552, 793, 1190
TfiI GAWTC 7 cut(s) 14, 131, 415, 1271, 1364, 2278, 2723
TscAI CASTG 6 cut(s) 537, 844, 1069, 1603, 1873, 1896
TseFI GTSAC 1 cut(s) 532
TseI GCWGC 9 cut(s) 20, 38, 44, 276, 1733, 2044, 2054, 2585, 2699
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 6 cut(s) 1656, 1662, 1716, 2352, 2542, 2591
TspGWI ACGGA 6 cut(s) 26, 67, 579, 1047, 1630, 1909
TspRI CASTG 6 cut(s) 537, 844, 1069, 1603, 1873, 1896
Tth111I GACNNNGTC 1 cut(s) 143
Van91I CCANNNNNTGG 1 cut(s) 1451
Vha464I CTTAAG 1 cut(s) 1802
VneI GTGCAC 1 cut(s) 864
VpaK11BI GGWCC 2 cut(s) 1370, 2199
VspI ATTAAT 1 cut(s) 1473
XapI RAATTY 5 cut(s) 331, 818, 2340, 2464, 2716
XceI RCATGY 5 cut(s) 225, 303, 602, 1823, 2549
XcmI CCANNNNNNNNNTGG 1 cut(s) 2003
XhoI CTCGAG 1 cut(s) 210
XmiI GTMKAC 2 cut(s) 450, 858
XmnI GAANNNNTTC 3 cut(s) 1393, 1663, 1747
XspI CTAG 5 cut(s) 999, 1979, 1991, 2135, 2532
ZraI GACGTC 1 cut(s) 448
ZrmI AGTACT 1 cut(s) 1192
Zsp2I ATGCAT 2 cut(s) 2551, 2656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.