MD09G1035200.v1.1

methyl-CpG-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004967
Physical Location & Seq
Reverse (-)
2150886 .. 2162549
11664 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1035200.v1.1.491

Sequence Viewer

Length: 6660 bp
ATGGAACTGACGGATTCAGCTGCCGACGAACTCAGAGCAGCGGCAGCCGCCGCCGCCAACGCCGAGCCAACTCGCTCCGTACTCAGCATCGATCTCAATGAGATCCCTTCTCCTTCCGAAACCCTACCCGATTCCTTCGACGTTGTCCGGTCTTACCACGACCACCCTTCGCCGCCTCCCGGCGGCCCGGCCGGAGTTCCCGGCTCAGCTCGAGGATCCGCATGCGCCGCCTGCGGCAAGCCCGAGGTTCGCGGGCACGTGGTGGTCTGCGACGGCTGCGAGCGCGGGTTTCACCTCACATGCGCCGGAATGCGCGGCCGGCAGGCGGTGAATTTGGTGGAGTGGGTCTGCGGGGACTGTCTGAGCGGTGGGGTTAGGAGCAAGCGGTGGCCGCTTGGAGTTAAGGCCAAGCAGATTCTCGATATCAACGCCTCGCCGCCGAGCGACGTCGACGACATCGCGGAAATGCGCGAATTGAGAAAGCACACTCCCGGTGGTAATTCTTTTGGTGGCAATCCATTTGGTGCCCCAGTGACAAATTCAAACTTCTTGTACTCTGGAAACGGATATGGCTTGGAAAAAGGTGCTGGGATAATGACACATGCGACTAAAGTGGGTTGGGAAGATATATTGCACTACACACAGACTACGGGTGGAAGCTTTGAGGAATTAAGTTCGAGGTTTCCGCTCGGGAAGCATAGTAATAACAATAATACAGCTATTAGAATACCATCCCGGAGTCCAGATGAGATAGTTCTACAGGCTCTTAGAGATTACGTTTCTGAAAGGCATGGCATACTGGAGGATGGTTGGCGTGTAGAATTTAAACAGTCAACAGCCAGTGGTGAACCATATATAGTATACCGTGCACCAAATGGGAAGACATTTGGCTCAGTATCTGAAGTTGCATATTTTCTTGGCTTGACGCCTAACTACAATTCCATGGGATCTGAAATAAGACGGGAGGGTTCTCTTTCTAATACTGAGAAGACTTATCTACCTAGAAAAAGAAAGTCAAGACTCTTATATGCCAATGGATTAGCTGAAAATAAGGAAAGTTTGCTCAGTGGCTATTGCAAGGAGCTCTCTTCCAATGGTATAAGTACAGAAGCTTTTGCCTGTGGTTTTGGAAATAATGTAAAACTTACTGAAGCAGGGACAGAAGAACATGGTTGCATTGGGTCTCGGCAAAGTACTGAGGGATTTCCGGTGCAGTTTGAAGATTTCTTTGTTCTTTCTTTGGGAGAAGTTGATACAAGGCCTTCTTATCATGATTCAAATCAGATCTATCCTGTAGGTTATAGGTCATGTTGGCATGATAAGGTTACTGGTTCTTTATTTGTGTGTGAAGTTTTAAATGGTGGTGATTCTGGACCTGTTTTTAAGGTCAGAAGATGTTCGTGTTCTGCCTTGCCGGTTCCCGAGGGTTCAACTATCCTCTGTAGGTCACAACTTGGCAACTTTTACAGTCAAATTAATCAAGAAAGTCATGACTTGACTTCTGATAATGATGGAAGTATCCAGATGATTCTGTCAGATCCTTCTCCACCTATGGAAAATGATATTTTGTCTTGCCTGAGGAGTTGTTCAGTTGAAGTCAGTGATGTTCAGACATCAGCTGAACCGCAGTTTGAAGATAATTCCGTTTATGAAAAATCTGGAACTCTTTCATCTGCTGATTTGGGTGTGATGGATGACATTGGTGAGATTTCAGTAGAAGACCATTCTTCATCTGCAGCTTGGGGAATGATTTCAAAAAAAATTGTTAATGCTTGTTCTGAAATATTTAAACAGAAGGGCATTCTTAAAGTTTTCTGTAAGCATGTTGAAAATGCTCAAGGCTCCCAAAATGAGGTTATAACAAATGACAGTGGCAACGTGAACCAATCTCCACTGGACAAGTTTTGTAGTTCAGCAGGTTCTGTCAGCATCTCTTCTGAACTTCGGGCCGATGATGAGCCTGGCTTTTCTTATGACGTACTAGCAAATTGGCTAGATCAGGACAGATTTGGATTAGATGTGGATTTTGTGCAAGAACTTTTGGAGCAGCTTCCTGCTGCCCAATCCTGTTCTCAATATCAATTTCTTAACGATAGAAGTTCTAATTCTACACAGCTAACTGTTGGAAATGGGCTTCTAGTGGTTAAAATGGGAGCTGGATTACATGGTAAAGAAGAAGTGTTGGATGGTTTGTTTAGGAGGTCCAAAAAAGTGAAGTTGGTCAAGGATCACCTCAAGAATGATCATCCACCTCCTCATGGGAAGCCATTGTGCTTGAGGATTCCTCCTGCCCTTGTTGATGATGTTTATCAGGTTTGGGAATTATTGTCGCGCTTTGATGAAATTTTGGGTCTAAAGGAGGCTTTTTCATTGGAAGAACTTGAGGAGGAACTTATTGACCCTTGGTTCGGCAGGTCAGATCTTTCAGAGAAGTTCGATAGGGAAAACCAGGGCACTCAAGCTTTAAATTCAAATAGAATTGATTATTCAAGTGGTCAATTATCTTTGACCATGGAGTCTGGCTCGACCGTTTCTAGGAATAATCCACATGCATTCATACATATGGAAACTGGAGCAATGAAGGAGGCAGCTCAAGATAAACTTGCATCTGTTACTTATAGCAGATGTTCTGGCATAGCTTTGACGAAGGCTCATGCATCACTGCTAAGAGTGCTCATAGGTGAGTTGCAGTTTAAGGTTGCTGCCCTGGTGGATCCGAATTTTGATTCTGGAGATCTGAAGTCAAAACGGGGAAGGAAGAAAGACATTGATATTTCAATTCCTGTGAAAAGAGCGAAGGTTAATATACTCCCAATTAATGAACTGACTTGGCCAGAGTTAGCACGGAGATACATCTTGGCTGTATTATCCATGGATGGCAACCTTGACTCGCCTGAGATTACCGCTCGTGAAAGCAGTAAAGTGTTTCGCTGCTTACAAGGTGACGGTGGGGTGCTCTGTGGGTCCTTATCTGGAGTAGCTGGGATGGAAGCAGATGCACTTTTGCTTGCAGAGGCTACAAAGCAAATTTTTGCTTCACTTAACAGAGAGCATGATGTATTTACCATAGAAGAGGAGGTGTCTGATGGACCAGCTGCTGTTGATAAGAATTTGGGGAATGATGGTAATAAGCCACTATGGGCGCAGGTGTTGGAACCTGTTAGAAAGCTACCGACAAATGTCGGAACAAGAATCAGAAAATGTGTTTATGAGGCTTTGGAAAAGGATCCACCTGAATGGGCGAGGAAAATACTGGAACATTCAATCAGTAAAGAAGTTTACAAGGGCAATGCATCAGGGCCTACAAAGAAAGCTGTTCTGTCACTGCTAGCAGATGTATCGGGTAAAGCATTGCCACAAAAGGCTGAGAAGGGAAGAAAACGGAAGATAAATGTCTCTATTTATGATGTTATCATGAAACAATGCCGCATTGTATTGCGTCGTGCTGCTGCTGCAGATGACACAAAGGTTTTCTGCAATTTGCTCGGGAGAAAGCTAATGAATTCAAGTGATAACGATGATGAGGGTCTTCTAGGATTGCCAGCCATGGTGTCTCGACCTTTGGACTTCAGGACTATTGATTTGAGATTGGCAGCTGGATCCTATGGGGGATCACATGAAGCCTTTCTAGAGGATGTCAGGGAGTTATGGAGCAATCTACGTATTGCTTATGGGGATAAGCCTGATTTGGTTGAGTTAACTGAGAAGTTAGCCCAAAATTTTGAAACATTATACGAGGAGGAGGTTGTCCCTCTTGTTCATAAGCTTGCAGAGTATTCAAAGTTGGAAGGCCTCAGTTCTGAAAGGAAAAAGGAAATTGATGATTTACTTACTTTCACAAATGGGATTCCCAAAGCCCCTTGGGATGAGGGAGTTTGCAAAGTATGCGGCATTGATAAAGATGACGACAGTGTACTGTTGTGTGATACTTGTGATTCTGAGTATCATACATACTGTTTGAATCCTCCTCTTGCAAGGATCCCGGACGGAAACTGGTATTGCCCCTCTTGTGTTGTTTCTAAACAAATAGTTCAAGATGCATCAAAACATCGTCTGGTCATTAGAAGACGTCGTAAAAATTACCAGGGAGAAGCTACCCGGGTTTTCTTGGAGACACTTGCACATTTAGCAGTGAAAATGGAAGAGAGCGAGTATTGGGATATCAACGTTGATGAGAGAACATTCCTGCTGAAGTTCTTATGTGATGAGTTGCTTAGTTCAGCAGTTATTCGTCAACACCTTGAATATTGTTCTGAGACATCAACCGAGTTGCATCAGAAATTGCGTTCTTTATCTGTAGAATGGAAGGTCCTCAGGTCTAGACAAGAAATTTTGGTTTCAAGAGCTGCAAAGGTCGATGCTGGTGACGGTACAAAGGAAGGGATTTCGGCTTCGGTTGAAACCAATGAAAGATGTCTTCATCAACCACAGGTTTTGAGTGGCAGGTCTAACTCCTTCAATGCAGTCTCTGATGATTCGGCACTAGAGGGTGCTCAAGGGTTTGATAAACACCCATCTGTCAGTAATGCAGAATATAATAGCCAACATTCTGTAGACACTGAAGTTAGGGAAAAAGATGTCCATTCTGCTTCAGATGACATCTCTGCACCTGGGAAGTTTTCTTCACATATGACTGCCGATAAAAGTGAAATATCCTCTAGACAAATTGAGTTTCCTTCATCAAATTGTTTGCCACATGAAATCAATGGGTCTAGTAAAGAAATCGGTTGCGTTGATCATCCACAAGACAATGCGGAAATGGATGTTTCTCTGCCTATAGATCAGAAAGGAATTAGCAACCCTTCAGATGTGAGGAGTAATCATGTGGGAGAGCAGATGTCACCTGCTTCTGTGAATGAATCAGAGTCCTACCACCTTGAGTTGAACTCTGTCAAAAATGATTTATCAGTTTTGGAGGATTTGATTGCGAGTACAGAATTCGACCTCTTAAAGGTGTCGGTGCGGAGAGAGTTTTTAGGAACTGATTCCCTTGGTGGCTTATACTGGGTTTCAGTTCTTCCGGGTGGACATGCTTGGATTATTGTAGATCGAAGTGTGTCATTTAAACATGGTATAAATATGAAAGATTGCAGAGACCCTGTATGGAGGAGTTCTGTTACACAGAGTTGTGGTCCAAACAACAGCATTTCCTTTAGGGCACCTTGGGTTTCTTATCAAACTGATACAGAAATTGAAGAACTTATGGGTTGGTTAAAGAACAAGCACCCTAAGGAAAGAGAGCTGAGGGACTCAATTCTGCACTGGAAAAGGTTGAGGTTCCAAAAGTTTGAGAAAATTAGAAGCCGAGGTCAGGATGATCACCTAACTGCTATTTCAGTGACTAGAAATGCTGATAAAACTGAAATTTCTGATCGTCTTGGTACTAGGGCAGCCACTTTGCTGAAGAAGATGTATGGTCCATGCTCCGAGATGGAAACCACCGACATCTCCAAAAAGTGGGGAAAGAGAGCTAGAGTAACCAATGATGAGAAAGGGTACCGGTGTGAATGCTTGGAACCCATTTGGCCCAGTAGACACCATTGCCTCTCTTGCCACAGAACCTTCTCCACTGATGCTGAACTTGAGGGGCATAACAATGGTAGGTGCATTCCAACTTCAGCAGCCTGTGAGAAGGGAAGGGAAATAGGTGATTCTTCTAAAGTTAAGGGGAGTATGAAGTGTGAGATGAATCGGGAAGAGGGCAGAGGTGAACTGAACAGTGTTGAAACTTCTAAAAGTGCTTGTTCAGAGCTTAGTGCAAAGTTGATTAAGTTTCAAAATGGAAGTTTAGGTTGCCCATATGATTTTGAAGAGATCTGCTCCAAGTTTGTGACCAATGATTCAAACAAGGATTTGATACAGGAAATAGGTCTCATAGGTTCACAAGGAGTTCCATCTTTTGCCCCAGCATCTCCTTATCTGAGTGATTCTACGCTAGCGACAATCTCTCAGAAAGATGTCGGTGTATCTGGTAATGGACTGGGGCCTGCCGAACAGCTAGTTTCACAAGGAAAGACTAATGTTGATATTGTAAGCAGTAACAATCTATCCTGGACAGGTGATGGAATGATGTTACTAAATGCTAACAAACTAACTTTGGGATCTCTGGAACGAGGGGAGGAAGGACATTCAAATAGCCATTCTTCTGTTGTGGGGGCTGGTCGTTTCTGTGTGGTCCCCCAGTCTTCTTTGAGGCCCTTGGTTGGCAAAGTTTGTCAAATTTATAAGAGACTCAAGATCAATCTGCTTGATATCGATGCTGCAGTCCCTGAGGAAGCTCTAAGACCATCAAAGGCACAGTTGGAAAGGAGATGGGCCTGGCGTGCGTTTGTTAAATCAGCAGTGACAATATATGAAATAGTTCAGGCAATGATTGTGCTGGAGGACATGATAAAGACTGAGTATCTAAGGAATGAGTGGTGGTATTGGTCATCATTTTCAGCTGCTGCCAAAATTTCAACTATCTCTGCACTTGCTCTGCGTATATACTCTCTTGATGCTGCTATCCTGTATGAGAAGATGCATCCTAGTTCCAATCTGACCGACAAGTTGGAGCCCAGCAGCGCACTGGACCTGAAGCCGCTTCCTGTTTTGGATTCAGCAGAAAAAACAAGGTCAAGCAGGAAATCTAACAAGAAAAGGAAGGAGACAGAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0001763 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004402 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005911 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008080 GO:0008150 GO:0008152 GO:0008327 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009648 GO:0009653 GO:0009791 GO:0009889 GO:0009987 GO:0010016 GO:0010216 GO:0010223 GO:0010228 GO:0010346 GO:0010468 GO:0010556 GO:0016043 GO:0016407 GO:0016410 GO:0016567 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022414 GO:0030054 GO:0031323 GO:0031326 GO:0031935 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0034212 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043565 GO:0043966 GO:0043967 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048367 GO:0048573 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051128 GO:0051171 GO:0051252 GO:0051276 GO:0055044 GO:0060255 GO:0060968 GO:0061458 GO:0061630 GO:0061659 GO:0061733 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0097159 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905393 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromodomain DDT FYRC MBD PHD WHIM1 WSD

Protein Analysis

2220

Amino Acids

244.54

Weight (kDa)

5.58

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 75 - 120 1.7e-07 PHD-finger
MBD PF01429 261 - 312 7.9e-07 Methyl-CpG binding domain
FYRN PF05964 402 - 436 9.2e-06 F/Y-rich N-terminus
PHD PF00628 1293 - 1337 2e-11 PHD-finger
WHIM1 PF15612 1371 - 1415 4.3e-12 WSTF, HB1, Itc1p, MBD9 motif 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1859, 6225
AarI CACCTGC 2 cut(s) 3136, 4852
AasI GACNNNNNNGTC 1 cut(s) 2515
AatII GACGTC 2 cut(s) 450, 4072
Acc36I ACCTGC 5 cut(s) 1907, 2403, 3136, 4434, 4852
Acc65I GGTACC 1 cut(s) 5496
AccB1I GGYRCC 3 cut(s) 524, 5158, 5496
AccB7I CCANNNNNTGG 1 cut(s) 5457
AccBSI CCGCTC 3 cut(s) 366, 688, 2906
AccI GTMKAC 4 cut(s) 450, 861, 4554, 5533
AccII CGCG 6 cut(s) 252, 285, 315, 461, 471, 2332
AclI AACGTT 1 cut(s) 4167
AcoI YGGCCR 4 cut(s) 189, 316, 389, 2831
AcvI CACGTG 1 cut(s) 259
AcyI GRCGYC 3 cut(s) 447, 926, 4069
AdeI CACNNNGTG 1 cut(s) 262
AgeI ACCGGT 1 cut(s) 5499
AjnI CCWGG 7 cut(s) 1960, 2448, 2706, 4083, 4609, 6050, 6317
Alw21I GWGCWC 5 cut(s) 871, 1086, 2676, 2958, 4495
Alw44I GTGCAC 1 cut(s) 867
AlwNI CAGNNNCTG 6 cut(s) 899, 1922, 3098, 4469, 6269, 6446
Ama87I CYCGRG 6 cut(s) 210, 242, 689, 1421, 3485, 4098
ApaLI GTGCAC 1 cut(s) 867
ArsI GACNNNNNNTTYG 4 cut(s) 2390, 2422, 3565, 3597
AseI ATTAAT 2 cut(s) 1476, 2817
AsiGI ACCGGT 1 cut(s) 5499
Asp700I GAANNNNTTC 6 cut(s) 1396, 1666, 1750, 3624, 4984, 6360
Asp718I GGTACC 1 cut(s) 5496
AspLEI GCGC 8 cut(s) 227, 285, 305, 315, 471, 2334, 3145, 6566
AsuC2I CCSGG 9 cut(s) 180, 188, 201, 492, 736, 3983, 4099, 4100, 5022
AsuNHI GCTAGC 2 cut(s) 3328, 5935
AvaI CYCGRG 6 cut(s) 210, 242, 689, 1421, 3485, 4098
AvaII GGWCC 9 cut(s) 1373, 2202, 2964, 3089, 4309, 5132, 5417, 6175, 6571
AxyI CCTNAGG 4 cut(s) 1577, 4313, 5229, 6270
BaeGI GKGCMC 5 cut(s) 258, 529, 871, 2456, 5161
BaeI ACNNNNGTAYC 2 cut(s) 5488, 5521
BalI TGGCCA 1 cut(s) 2833
BamHI GGATCC 5 cut(s) 215, 2713, 3226, 3599, 3978
BanI GGYRCC 3 cut(s) 524, 5158, 5496
BanII GRGCYC 2 cut(s) 1086, 6558
BauI CACGAG 1 cut(s) 2907
BbrPI CACGTG 1 cut(s) 259
BbsI GAAGAC 7 cut(s) 887, 995, 1725, 3521, 4072, 4409, 6177
Bbv12I GWGCWC 5 cut(s) 871, 1086, 2676, 2958, 4495
BbvCI CCTCAGC 1 cut(s) 5243
BceAI ACGGC 1 cut(s) 289
BcgI CGANNNNNNTGC 4 cut(s) 3321, 3355, 3466, 3500
BciT130I CCWGG 7 cut(s) 1962, 2450, 2708, 4085, 4611, 6052, 6319
BciVI GTATCC 1 cut(s) 1529
BclI TGATCA 3 cut(s) 2242, 4735, 5317
BcnI CCSGG 9 cut(s) 180, 188, 201, 492, 736, 3983, 4099, 4100, 5022
BfmI CTRYAG 9 cut(s) 758, 1293, 1441, 1734, 3453, 4296, 4551, 4776, 6261
BfuAI ACCTGC 5 cut(s) 1907, 2403, 3136, 4434, 4852
BfuI GTATCC 1 cut(s) 1529
BglII AGATCT 4 cut(s) 1284, 2419, 2734, 5814
BlpI GCTNAGC 1 cut(s) 205
BmcAI AGTACT 1 cut(s) 1195
Bme18I GGWCC 9 cut(s) 1373, 2202, 2964, 3089, 4309, 5132, 5417, 6175, 6571
BmeT110I CYCGRG 6 cut(s) 210, 242, 689, 1421, 3485, 4098
BmrI ACTGGG 5 cut(s) 524, 5014, 5523, 5990, 6175
BmtI GCTAGC 2 cut(s) 3332, 5939
BmuI ACTGGG 5 cut(s) 524, 5014, 5523, 5990, 6175
BoxI GACNNNNGTC 1 cut(s) 3179
BpiI GAAGAC 7 cut(s) 887, 995, 1725, 3521, 4072, 4409, 6177
BplI GAGNNNNNCTC 8 cut(s) 2269, 2301, 2507, 2539, 4871, 4903, 5804, 5836
BpmI CTGGAG 5 cut(s) 821, 2592, 2751, 2994, 6401
Bpu10I CCTNAGC 1 cut(s) 5243
Bpu1102I GCTNAGC 1 cut(s) 205
BpuMI CCSGG 9 cut(s) 180, 188, 201, 492, 736, 3983, 4099, 4100, 5022
Bsa29I ATCGAT 2 cut(s) 90, 6255
BsaAI YACGTR 2 cut(s) 259, 3662
BsaBI GATNNNNATC 2 cut(s) 1278, 2307
BsaHI GRCGYC 3 cut(s) 447, 926, 4069
BsaI GGTCTC 3 cut(s) 1188, 5088, 5876
BsaWI WCCGGW 3 cut(s) 147, 1207, 5499
Bse118I RCCGGY 3 cut(s) 318, 1414, 5499
Bse21I CCTNAGG 4 cut(s) 1577, 4313, 5229, 6270
Bse3DI GCAATG 5 cut(s) 2583, 3295, 3350, 5539, 6375
Bse8I GATNNNNATC 2 cut(s) 1278, 2307
BseBI CCWGG 7 cut(s) 1962, 2450, 2708, 4085, 4611, 6052, 6319
BseCI ATCGAT 2 cut(s) 90, 6255
BseJI GATNNNNATC 2 cut(s) 1278, 2307
BseMI GCAATG 5 cut(s) 2583, 3295, 3350, 5539, 6375
BseRI GAGGAG 9 cut(s) 1594, 2244, 2399, 3089, 3752, 3755, 3957, 4828, 5122
BseSI GKGCMC 5 cut(s) 258, 529, 871, 2456, 5161
BseX3I CGGCCG 2 cut(s) 189, 316
BseYI CCCAGC 4 cut(s) 587, 2981, 5905, 6557
BsgI GTGCAG 4 cut(s) 1232, 4590, 5243, 6453
Bsh1236I CGCG 6 cut(s) 252, 285, 315, 461, 471, 2332
Bsh1285I CGRYCG 3 cut(s) 192, 319, 2529
BshNI GGYRCC 3 cut(s) 524, 5158, 5496
BshTI ACCGGT 1 cut(s) 5499
BshVI ATCGAT 2 cut(s) 90, 6255
BsiEI CGRYCG 3 cut(s) 192, 319, 2529
BsiHKAI GWGCWC 5 cut(s) 871, 1086, 2676, 2958, 4495
BsiHKCI CYCGRG 6 cut(s) 210, 242, 689, 1421, 3485, 4098
BslFI GGGAC 6 cut(s) 368, 1171, 3734, 5261, 6161, 6251
BsmFI GGGAC 6 cut(s) 368, 1171, 3734, 5261, 6161, 6251
BsmI GAATGC 5 cut(s) 315, 1800, 2552, 5513, 5607
Bso31I GGTCTC 3 cut(s) 1188, 5088, 5876
BsoBI CYCGRG 6 cut(s) 210, 242, 689, 1421, 3485, 4098
Bsp1720I GCTNAGC 1 cut(s) 205
Bsp19I CCATGG 4 cut(s) 942, 2511, 2871, 3546
BspDI ATCGAT 2 cut(s) 90, 6255
BspFNI CGCG 6 cut(s) 252, 285, 315, 461, 471, 2332
BspHI TCATGA 3 cut(s) 1270, 1489, 3414
BspMAI CTGCAG 3 cut(s) 1738, 3457, 6265
BspMI ACCTGC 5 cut(s) 1907, 2403, 3136, 4434, 4852
BspOI GCTAGC 2 cut(s) 3332, 5939
BspT107I GGYRCC 3 cut(s) 524, 5158, 5496
BspTNI GGTCTC 3 cut(s) 1188, 5088, 5876
BsrBI CCGCTC 3 cut(s) 366, 688, 2906
BsrDI GCAATG 5 cut(s) 2583, 3295, 3350, 5539, 6375
BsrFI RCCGGY 3 cut(s) 318, 1414, 5499
BssAI RCCGGY 3 cut(s) 318, 1414, 5499
BssNAI GTATAC 1 cut(s) 862
BssNI GRCGYC 3 cut(s) 447, 926, 4069
BssSI CACGAG 1 cut(s) 2907
BssT1I CCWWGG 9 cut(s) 942, 2402, 2511, 2871, 3546, 3860, 4990, 5162, 6198
Bst1107I GTATAC 1 cut(s) 862
Bst2BI CACGAG 1 cut(s) 2907
Bst2UI CCWGG 7 cut(s) 1962, 2450, 2708, 4085, 4611, 6052, 6319
Bst6I CTCTTC 6 cut(s) 1093, 1939, 3066, 4137, 5691, 5805
BstACI GRCGYC 3 cut(s) 447, 926, 4069
BstAPI GCANNNNNTGC 1 cut(s) 3885
BstBAI YACGTR 2 cut(s) 259, 3662
BstDSI CCRYGG 4 cut(s) 942, 2511, 2871, 3546
BstENI CCTNNNNNAGG 1 cut(s) 4949
BstFNI CGCG 6 cut(s) 252, 285, 315, 461, 471, 2332
BstHHI GCGC 8 cut(s) 227, 285, 305, 315, 471, 2334, 3145, 6566
BstMCI CGRYCG 3 cut(s) 192, 319, 2529
BstNI CCWGG 7 cut(s) 1962, 2450, 2708, 4085, 4611, 6052, 6319
BstNSI RCATGY 6 cut(s) 225, 303, 605, 1826, 2552, 5033
BstPAI GACNNNNGTC 1 cut(s) 3179
BstSFI CTRYAG 9 cut(s) 758, 1293, 1441, 1734, 3453, 4296, 4551, 4776, 6261
BstSLI GKGCMC 5 cut(s) 258, 529, 871, 2456, 5161
BstSNI TACGTA 1 cut(s) 3662
BstUI CGCG 6 cut(s) 252, 285, 315, 461, 471, 2332
BstV2I GAAGAC 7 cut(s) 887, 995, 1725, 3521, 4072, 4409, 6177
BstXI CCANNNNNNTGG 1 cut(s) 3237
BstZ17I GTATAC 1 cut(s) 862
BstZI CGGCCG 2 cut(s) 189, 316
Bsu15I ATCGAT 2 cut(s) 90, 6255
Bsu36I CCTNAGG 4 cut(s) 1577, 4313, 5229, 6270
BsuI GTATCC 1 cut(s) 1529
BsuTUI ATCGAT 2 cut(s) 90, 6255
BtgI CCRYGG 4 cut(s) 942, 2511, 2871, 3546
BtgZI GCGATG 1 cut(s) 442
BtsI GCAGTG 4 cut(s) 2660, 3323, 4137, 6348
BveI ACCTGC 5 cut(s) 1907, 2403, 3136, 4434, 4852
CaiI CAGNNNCTG 6 cut(s) 899, 1922, 3098, 4469, 6269, 6446
CciI TCATGA 3 cut(s) 1270, 1489, 3414
CfoI GCGC 8 cut(s) 227, 285, 305, 315, 471, 2334, 3145, 6566
Cfr10I RCCGGY 3 cut(s) 318, 1414, 5499
Cfr9I CCCGGG 1 cut(s) 4098
ClaI ATCGAT 2 cut(s) 90, 6255
CseI GACGC 2 cut(s) 934, 3428
CspAI ACCGGT 1 cut(s) 5499
CspCI CAANNNNNGTGG 2 cut(s) 4416, 4451
DraI TTTAAA 5 cut(s) 826, 1356, 1789, 2466, 5065
DraIII CACNNNGTG 1 cut(s) 262
DrdI GACNNNNNNGTC 1 cut(s) 2515
DseDI GACNNNNNNGTC 1 cut(s) 2515
EaeI YGGCCR 4 cut(s) 189, 316, 389, 2831
EagI CGGCCG 2 cut(s) 189, 316
Eam1104I CTCTTC 6 cut(s) 1093, 1939, 3066, 4137, 5691, 5805
EarI CTCTTC 6 cut(s) 1093, 1939, 3066, 4137, 5691, 5805
Ecl136II GAGCTC 1 cut(s) 1084
EclXI CGGCCG 2 cut(s) 189, 316
Eco105I TACGTA 1 cut(s) 3662
Eco130I CCWWGG 9 cut(s) 942, 2402, 2511, 2871, 3546, 3860, 4990, 5162, 6198
Eco147I AGGCCT 2 cut(s) 1261, 3792
Eco24I GRGCYC 2 cut(s) 1086, 6558
Eco31I GGTCTC 3 cut(s) 1188, 5088, 5876
Eco32I GATATC 3 cut(s) 424, 4162, 6253
Eco47I GGWCC 9 cut(s) 1373, 2202, 2964, 3089, 4309, 5132, 5417, 6175, 6571
Eco52I CGGCCG 2 cut(s) 189, 316
Eco53kI GAGCTC 1 cut(s) 1084
Eco72I CACGTG 1 cut(s) 259
Eco81I CCTNAGG 4 cut(s) 1577, 4313, 5229, 6270
Eco88I CYCGRG 6 cut(s) 210, 242, 689, 1421, 3485, 4098
EcoICRI GAGCTC 1 cut(s) 1084
EcoNI CCTNNNNNAGG 1 cut(s) 4949
EcoO109I RGGNCCY 5 cut(s) 2964, 3299, 4309, 5984, 6195
EcoRI GAATTC 2 cut(s) 3502, 4937
EcoRII CCWGG 7 cut(s) 1960, 2448, 2706, 4083, 4609, 6050, 6317
EcoRV GATATC 3 cut(s) 424, 4162, 6253
EcoT14I CCWWGG 9 cut(s) 942, 2402, 2511, 2871, 3546, 3860, 4990, 5162, 6198
EcoT22I ATGCAT 5 cut(s) 2554, 2659, 3295, 4042, 6525
EcoT38I GRGCYC 2 cut(s) 1086, 6558
ErhI CCWWGG 9 cut(s) 942, 2402, 2511, 2871, 3546, 3860, 4990, 5162, 6198
FalI AAGNNNNNCTT 4 cut(s) 1249, 1281, 2586, 2618
FaqI GGGAC 6 cut(s) 368, 1171, 3734, 5261, 6161, 6251
FauI CCCGC 3 cut(s) 245, 278, 344
FauNDI CATATG 3 cut(s) 2562, 4629, 5800
FbaI TGATCA 3 cut(s) 2242, 4735, 5317
FblI GTMKAC 4 cut(s) 450, 861, 4554, 5533
FriOI GRGCYC 2 cut(s) 1086, 6558
GlaI GCGC 8 cut(s) 226, 284, 304, 314, 470, 2333, 3144, 6565
GsaI CCCAGC 4 cut(s) 591, 2985, 5909, 6561
GsuI CTGGAG 5 cut(s) 821, 2592, 2751, 2994, 6401
HgaI GACGC 2 cut(s) 934, 3428
HhaI GCGC 8 cut(s) 227, 285, 305, 315, 471, 2334, 3145, 6566
Hin1I GRCGYC 3 cut(s) 447, 926, 4069
Hin6I GCGC 8 cut(s) 225, 283, 303, 313, 469, 2332, 3143, 6564
HinP1I GCGC 8 cut(s) 225, 283, 303, 313, 469, 2332, 3143, 6564
HincII GTYRAC 4 cut(s) 451, 834, 3699, 4235
HindII GTYRAC 4 cut(s) 451, 834, 3699, 4235
HindIII AAGCTT 4 cut(s) 658, 1110, 2460, 3764
HpaI GTTAAC 1 cut(s) 3699
Hpy99I CGWCG 8 cut(s) 29, 143, 275, 449, 452, 455, 3444, 4074
HpyCH4IV ACGT 9 cut(s) 141, 258, 447, 777, 1878, 1977, 3661, 4069, 4167
HpySE526I ACGT 9 cut(s) 141, 258, 447, 777, 1878, 1977, 3661, 4069, 4167
Hsp92I GRCGYC 3 cut(s) 447, 926, 4069
HspAI GCGC 8 cut(s) 225, 283, 303, 313, 469, 2332, 3143, 6564
KpnI GGTACC 1 cut(s) 5500
KroI GCCGGC 1 cut(s) 318
KroNI GCCGGC 1 cut(s) 320
Ksp22I TGATCA 3 cut(s) 2242, 4735, 5317
KspAI GTTAAC 1 cut(s) 3699
MaeII ACGT 9 cut(s) 141, 258, 447, 777, 1878, 1977, 3661, 4069, 4167
MbiI CCGCTC 3 cut(s) 366, 688, 2906
MlsI TGGCCA 1 cut(s) 2833
MluNI TGGCCA 1 cut(s) 2833
MlyI GAGTC 7 cut(s) 748, 1014, 2525, 2882, 4874, 5243, 6225
MmeI TCCRAC 8 cut(s) 2104, 2163, 3132, 3163, 3765, 5636, 6282, 6531
Mox20I TGGCCA 1 cut(s) 2833
Mph1103I ATGCAT 5 cut(s) 2554, 2659, 3295, 4042, 6525
MroNI GCCGGC 1 cut(s) 318
MroXI GAANNNNTTC 6 cut(s) 1396, 1666, 1750, 3624, 4984, 6360
MscI TGGCCA 1 cut(s) 2833
MslI CAYNNNNRTG 3 cut(s) 2561, 3235, 5595
Msp20I TGGCCA 1 cut(s) 2833
MspA1I CMGCKG 6 cut(s) 20, 41, 1619, 3095, 3596, 6443
Mva1269I GAATGC 5 cut(s) 315, 1800, 2552, 5513, 5607
MvaI CCWGG 7 cut(s) 1962, 2450, 2708, 4085, 4611, 6052, 6319
MvnI CGCG 6 cut(s) 252, 285, 315, 461, 471, 2332
NaeI GCCGGC 1 cut(s) 320
NciI CCSGG 9 cut(s) 180, 188, 201, 492, 736, 3983, 4099, 4100, 5022
NcoI CCATGG 4 cut(s) 942, 2511, 2871, 3546
NdeI CATATG 3 cut(s) 2562, 4629, 5800
NgoMIV GCCGGC 1 cut(s) 318
NheI GCTAGC 2 cut(s) 3328, 5935
NmeAIII GCCGAG 4 cut(s) 88, 465, 1165, 5330
NmuCI GTSAC 9 cut(s) 532, 1446, 2942, 3321, 4364, 4839, 5338, 5830, 6343
NsiI ATGCAT 5 cut(s) 2554, 2659, 3295, 4042, 6525
NspI RCATGY 6 cut(s) 225, 303, 605, 1826, 2552, 5033
PaeI GCATGC 1 cut(s) 225
PaeR7I CTCGAG 1 cut(s) 210
PagI TCATGA 3 cut(s) 1270, 1489, 3414
PaqCI CACCTGC 2 cut(s) 3136, 4852
PceI AGGCCT 2 cut(s) 1261, 3792
PctI GAATGC 5 cut(s) 315, 1800, 2552, 5513, 5607
PdiI GCCGGC 1 cut(s) 320
PdmI GAANNNNTTC 6 cut(s) 1396, 1666, 1750, 3624, 4984, 6360
PflFI GACNNNGTC 1 cut(s) 143
PflMI CCANNNNNTGG 1 cut(s) 5457
PfoI TCCNGGA 3 cut(s) 734, 3981, 6050
PinAI ACCGGT 1 cut(s) 5499
PleI GAGTC 7 cut(s) 747, 1014, 2524, 2882, 4873, 5243, 6225
PmaCI CACGTG 1 cut(s) 259
PmlI CACGTG 1 cut(s) 259
PpsI GAGTC 7 cut(s) 747, 1014, 2524, 2882, 4873, 5243, 6225
Ppu21I YACGTR 2 cut(s) 259, 3662
PpuMI RGGWCCY 2 cut(s) 2964, 4309
PshAI GACNNNNGTC 1 cut(s) 3179
PshBI ATTAAT 2 cut(s) 1476, 2817
PsiI TTATAA 2 cut(s) 1859, 6225
Psp124BI GAGCTC 1 cut(s) 1086
Psp1406I AACGTT 1 cut(s) 4167
Psp5II RGGWCCY 2 cut(s) 2964, 4309
Psp6I CCWGG 7 cut(s) 1960, 2448, 2706, 4083, 4609, 6050, 6317
PspCI CACGTG 1 cut(s) 259
PspFI CCCAGC 4 cut(s) 587, 2981, 5905, 6557
PspGI CCWGG 7 cut(s) 1960, 2448, 2706, 4083, 4609, 6050, 6317
PspPPI RGGWCCY 2 cut(s) 2964, 4309
PspXI VCTCGAGB 1 cut(s) 210
PstI CTGCAG 3 cut(s) 1738, 3457, 6265
PstNI CAGNNNCTG 6 cut(s) 899, 1922, 3098, 4469, 6269, 6446
PsyI GACNNNGTC 1 cut(s) 143
PvuII CAGCTG 5 cut(s) 20, 1619, 3095, 3596, 6443
RseI CAYNNNNRTG 3 cut(s) 2561, 3235, 5595
SacI GAGCTC 1 cut(s) 1086
SalI GTCGAC 1 cut(s) 449
ScaI AGTACT 1 cut(s) 1195
SchI GAGTC 7 cut(s) 748, 1014, 2525, 2882, 4874, 5243, 6225
SfcI CTRYAG 9 cut(s) 758, 1293, 1441, 1734, 3453, 4296, 4551, 4776, 6261
Sfr274I CTCGAG 1 cut(s) 210
SgrDI CGTCGACG 1 cut(s) 449
SinI GGWCC 9 cut(s) 1373, 2202, 2964, 3089, 4309, 5132, 5417, 6175, 6571
SlaI CTCGAG 1 cut(s) 210
SmaI CCCGGG 1 cut(s) 4100
SmiMI CAYNNNNRTG 3 cut(s) 2561, 3235, 5595
SnaBI TACGTA 1 cut(s) 3662
SphI GCATGC 1 cut(s) 225
SseBI AGGCCT 2 cut(s) 1261, 3792
SspI AATATT 2 cut(s) 1785, 4247
SstI GAGCTC 1 cut(s) 1086
StuI AGGCCT 2 cut(s) 1261, 3792
StyI CCWWGG 9 cut(s) 942, 2402, 2511, 2871, 3546, 3860, 4990, 5162, 6198
TaiI ACGT 9 cut(s) 144, 261, 450, 780, 1881, 1980, 3664, 4072, 4170
TaqII GACCGA 1 cut(s) 6557
TatI WGTACW 5 cut(s) 552, 1103, 1193, 3913, 4931
TseFI GTSAC 9 cut(s) 532, 1446, 2942, 3321, 4364, 4839, 5338, 5830, 6343
Tsp45I GTSAC 9 cut(s) 532, 1446, 2942, 3321, 4364, 4839, 5338, 5830, 6343
TspGWI ACGGA 7 cut(s) 26, 67, 579, 1633, 2860, 3397, 4002
TspMI CCCGGG 1 cut(s) 4098
Tth111I GACNNNGTC 1 cut(s) 143
Van91I CCANNNNNTGG 1 cut(s) 5457
VneI GTGCAC 1 cut(s) 867
VpaK11BI GGWCC 9 cut(s) 1373, 2202, 2964, 3089, 4309, 5132, 5417, 6175, 6571
VspI ATTAAT 2 cut(s) 1476, 2817
XagI CCTNNNNNAGG 1 cut(s) 4949
XbaI TCTAGA 3 cut(s) 3628, 4319, 4658
XceI RCATGY 6 cut(s) 225, 303, 605, 1826, 2552, 5033
XcmI CCANNNNNNNNNTGG 1 cut(s) 6565
XhoI CTCGAG 1 cut(s) 210
XmaI CCCGGG 1 cut(s) 4098
XmiI GTMKAC 4 cut(s) 450, 861, 4554, 5533
XmnI GAANNNNTTC 6 cut(s) 1396, 1666, 1750, 3624, 4984, 6360
ZraI GACGTC 2 cut(s) 448, 4070
ZrmI AGTACT 1 cut(s) 1195
Zsp2I ATGCAT 5 cut(s) 2554, 2659, 3295, 4042, 6525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.