MD17G1035500.v1.1

methyl-CpG-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004967
Physical Location & Seq
Reverse (-)
2538255 .. 2550389
12135 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1035500.v1.1.491

Sequence Viewer

Length: 6693 bp
ATGGAACTCACCGATTCAGCTGCCGACGAACTCAGAGCAGCGGCAGCCGCCGCCGCCAACGCCGAGCCAACTCGCTCCGTACTCGGCATCGATCTCAATGAGATCCCTTCCCCTTCCGAAACCCTACCCGATTCCTTCGACGTCGTGAGGTCTTACCACGACCACCCCTCGCCGCCGCCCGGGGGCCTGGCCGGAGTTCCCGGCTCAGCTCAAGGATCCGCATGCGCCGCCTGCGGCAAGCCCGAGGTTCGCGGGAACGTGGTGGTCTGCGACGGCTGCGAGCGCGGGTTTCACCTCACATGCGCGGGAATGCGCGGCCGCCAGGCGGTGAATCTGGTGGAGTGGGTCTGTGGGGACTGTTTGAGCGGTGGGGTTAGGAGCAAGCGGTGGCCGCTTGGGGTTAAGGCCAAGCACATTCTCGATATCAACGCCTCGCCGCCGAGCGACGTCGACGAATTCCCGGAAATGCGCGAATTGAGAAAGCACACTCCCGGTGGTAATTCTTTTGGTGGCAATCCATTTGGTGCCCCAGTGACATATTCAAACTTCTCGTACTCTGGAAACGGATATGGGTTGGAAAAAGCTGCTGGGATAATGCCACACACAGCTAAAGTGGGCTTGGAAGATATATTGCACCATACACAGACTATGGGTGGAAGCTTTGAGGAATTAATTTCGAGGTTTTCGCTTGGAAAGCATAGTAATAACAATAATACAGCTATTAGAATGCCATCCCGGAGTCCAGATGAGATAGTTTTACAGGCTCTTAGAGATTATGTTTCTGAAAGGCATGGCGTACTGGAGGATGGTTGGCGTGTAGAATTTAGACAGTCAACAGCCAGTGGTGAACCGTATTTAGTATACTGTGCACCAAATGGGAAGACATTTGGTTCAGTATCTGAAGTTGCATATTTTCTTGGCTTGATGCCTAACTACAATTCCATGGGATCTGAAATAAAGAGGGAGGGGTCTCTTTCTAATACTGAGAAGACTTACGTATCTAGAAAAAGAAAGTCAAGACTCTTACATGCCAATGGATTAGCTGAAAATAAGGAAAGTTTGCTCAGAGGCTATTGCAAGGAGCTCCCTTCCAATGGTCTAAGTATGGAAGTTTCTGCCCGTTGTTTTGGAAATAATGTAAAACTTACAGAAGCTGGGACAGAAGAACATGGTTGCATTGGTTCTCGGCAAAGTGATGGGGGATTTCCGGTGCAGTTTGAAGATTTCTTTGTTCTTTCTTTGGGAGAAGTTGATACAAGGCCCTCTTATCATGATTCTAATCAGATCTATCCTGTAGGTTATAAGTCATGTTGGCATGATAAGGTTACTGGTTCTTTATTTGTGTGTGAAGTTTTGAATGGCGGTGATTCTGGACCTGTTTTTAAGGTCAGAAGATGTTCTTGTTCTGCCTTGCCACCCAAGGGTTCAACTATCCTCTGTAGGCCACAACTTGGCAACTTTTACAGTCAAATTAATCAAGAAAGTCATGACTTGACTTTTGATAATGATGGAAGTATCCAGATGATTCTGTCAGATCCTTCGCCTAAGGAAAATGATATTTTGTCTTGCCTGAGGAGTTGTTCAGTTGAAGCCAGTGATGTCCAGACATCAGCTGAACCGCAACTTGAAGATAATTCCGTTTATGAAAAATCTGGAACTCTTTCATCTGCTGATTTAGGTATGATGGATGACATTGGTGAGATATCAGTAGAAGACCATTCTTCATCTGCAGCTTGGGAAATGATTTCAAAAAAAGTTGTTAATGCTTGTTCTGAAATATTTAAACAGAAGGGCATTCTTAAATTTTTCTGTAAGCATGTTGAAAATGCTCAGCGCTTCACAAATGAGGTTATAAAAAATGACAGTTGCAAAGTGAACCAATCTCCATTGGACAAGTTTTGTAGTTCACCAGGTTCTGTCAGCATCTCTTCTGAATTTCGAGCCGATGATGACCCTGGCTCTTTTTATGACGTACTAGCAAATTGGCTAGACCAGGATAGATTTGGTTTAGATGTGGACTTTGTGCAAGAACTTTTGGAGCAGCTTCCTGGTGCCCAATCCTGTTCTCAATATCAATTTCTTAACAATAGAAGTTCTAATTCTACGCAGCTAACTGTTGGAAATGGGCTTCTAGTGGTTAAAATGGGGGCTGGATTACATGGTAAAGAAGAAGTGTTAGATGGTTTGTTTAGGAGGTCCAAAAAAGTGAAGTTGGCCCAGGATCACCTCAAGAGTGGTCATCCACCTCCTCTTGGGAAGCCATTGTGCTTCAGGATTCCTCTTGCCCTTTTTGGTGATGTTTATCAGGTTTGGGAATTATTATCGCGCTTTGATGAAATTTTGGGTCTAAAGGAGGCTTTTTCGTTGGAAGAACTTGAGGAGGAACTTATTAACCCTTGGTTCGGCAGGTCAGATTTTTCAGAGAAGTTCGATAGGGAAATCCAAGGCACTCAAGCGTTAAATGCAAATGGAATTGATTATTCAAGTACTCAATTATCTTCTACCATGGACTCTGGCTCGACAATTTCTAGGAATAATCCACATGCATTCATACATATGGAAACTGGAGCAATGAAGGAGGCAGCTCAAGACAAAGTTGCATCTGTTACTTATAGCAGATGTTCTGGCATAGCGTTGACAAAGGCTCACAGATCACTGCTAAGAGTGCTCATAGGTGAGTTGCAGTCTAAGGTTGCTGCCCTGGTAGATCCGAATTTTGATTCTGGAGATCTGAAGTCAAAACGGGGAAGGAAGAAAGATGTTGATATTTCAATTCCTGTGAAAAGAGCAAAGCTTAATATACTCCCAATTAATGAACTGACTTGGCCAGAGTTAGCACGGAGATACATCCTGGCTGTATTAGCCATGGATGGTAACCTTGACTCGCCTGAGATTACTGCTCGTGAAAGCAGTAAAGTGTTTCGCTGCTTACAAGGTGATGGTGGAGTGCTCTGTGGGTCTTTAACTGGAGTAGCTGGGATGGAAGCAGATGCACTTTTGCTTGCAGAGGCTACGAAGCAAATTTTTGCTTCACTTAACAGAGGGAATGATGTACTTACCATAGAAGAGGAGGTGTCTGATGGACCAGGGGCTGTCGAGAAGAATTTGGGGGATGATGGTAACAATCCGCTATGGGCGCAGGTGCTGGAACCTGTTAGAAAGCTACCGACAAATGTCGGAACAAGAATCAGAAAATGTGTTTATGAGGCTTTGGAAAAGGATCCACCTGAATGGGCGAGGAAAATACTGGAACATTCAATCAGTAAAGAAGTTTACAAAGGCAATGCATCAGGACCTACGAAGAAAGCGGTTCTGTCACTGCTAGCAGATGTATCGGGTAAAGCATTGCCACAAAAGGCTGAGAAGGGAAGAAAACGGAAGATTAATGTCTCTATTTGTGATGTTATCATGAAACAATGCCGCATTGTATTTCGTCGTGCTGCTGCTGCAGATGACACAAAGGTTTTCTGCAATTTGCTCGGAAGAAAGCTAATGAATTCAAGTGATAACGATGATGAGGGTCTTCTAGGATCCCCGGCCATGGTGTCTCGGCCTTTGGACTTCAGGACTATTGATTTGAGATTGGCAGCTGGATCCTATGGGGGATCACATGAAGCCTTTCTTGAGGATGTCAGGGAGTTATGGAGTAATCTACGTATTGCGTATGGGGATCAGCCTGATTTGGTTGAGTTGACTGAGAAGTTAGCCCAAAATTTTGAAACATTGTACGAGGAGGAGGTTGTCCCTCTTGTTCATAAGCTTGCGGAGTATTCAAAGTTGGAAGGCCTAAGTTCTGAAAGGAGAAAGGAAATTGATGATTTACTTGCTTTCACAAATGGGATTCCCAAAGCCCCTTGGGATGAGGGAGTATGCAAAGTATGTGGCATTGATAAAGATGACGACAGCGTACTGTTGTGTGATACTTGTGATGCTGAGTATCATACTTACTGTTTGAATCCTCCTCTTGCAAGGATCCCGGAGGGAAACTGGTATTGCCCCTCTTGTGTTGTTTCTAAACAAATTGTTCAAGATGCATTGCAACATCGTCATGTCATTAGAAGACGACGTAAAAATTACCAGGGAGAAGCTATCCGAGTTTTCTTGGAGACACTTGCACATTTAGCAGCGAAAATGGAAGAGAGCGAGTATTGGGATATCAACTTGGACGAGAGAACATTCCTGCTGAAGTTCTTATGTGATGAGTTGCTTAATTCAGCAGTTATCCGTCAGCACCTTGAGTATTGTTCTGAGACATCGACTGAATTGCAGCAGAAATTGCGTTCTTTATCTGTAGAATGGAAGGTCCTCAGGTCCAGACAAGAAATTTTGGTTTCAAGAGCTGCAAGGGTCGATGCTAGTGCCAATATAAGGGAAGGGCTTTCGGCTTCAGTTGAAACCAATGAAAGATGTCTTCGTCAATCACAGGCTTTGAGTGGCAGGTCTAACTCCTTAAATGCAGTCTCTGATGATTCGGCACTAGAGGGTGCTCAAGGGATTGATAAATACCCATCTGTCAGTAATGCAGAATATAATAGCCAACATATTGTAGACACTGAAGTTAGGGAAAAAGATGTCCATGCTGCTTCAGATGACATCTCTGCACCTGGGAAGTATTCTTCAAATATGGCTTCTGATAAAAGCGAAATATCCTCTAGACAAATCGAGTTACCTTCATCAAATTGTTTGCCACATGAAATCAATGGGCCTAGTAAAGAAGTAGGTTGTGTTGGTCATCCACAAGACGATGTTGAAATGGATGTTTCTCTGCCTTTAGATCAGAAAGGACTTAGTATCCCGTCAGATGTGAGGAGTAATCATGTGGGAGAGCAGATGTCACCTGCTTCTGTGAATGAATCACAATCCTACCACCTTGAGTTGAACTCTGTCAAAACTGATTTATCCCTTTTGGAGGATTCGATTGCCAGTACAGAATTCGAGCTTCTAAAGGTGTCAGTTCGGAGGGAGTTCCTAGGAATTGATTCCCTTGGTGGTTTATACTGGGCTTCAGCTCTTCTGGGCGGACATGGTCGGATTATTGTAGATCGAAGTGTGTCATTGAAGCATGGTATAAATGTGAAAGATTGCAGAGACTCTGTGTGGAGTTCTGTTGCACAGAGTTGTGGGCCATCTGGTATGGGTAGCTTGCCGTTAGAGGGGTCCAAAGTTGGCTGTCCGTATTTATTTGAACCGAACAACAGCATTTCGTTTAGTGCCCCTTGGGTTTCTTATCAAACTGATGCAGAAATTGAAGAACTTATAGGTTGGTTAAAGAACAAGCACCCTAAGGAAAGAGAGCTGAGGGACTCGATTCTGCACTGGAAAAGGTCAAGATTCCGAAAGTTTCAGAAAATTAGAAGCCAAGGACAGGATGAGCTCCTAACTACTATTTCAGTGACTAGAAATGCTGATAAAACTGAAATTTCTGATTGTCTTGGTACTAGGGCAGCCACTTTGCTGGAGGAGCTGTATGGTCCATGCTCCGAGATGGAAACCGCCGACATCTCCAAAAAGTGGGGAAAGAGAGCTAGAGTAACCAATGATGAGAAAGGGTACCGGTGTGAATGTTTGGAACCCATTTGGCCCAGTAGACACCATTGCCTATCTTGCCACAGAACCTTCTTCACGGATGCTGAACTTGAAGGGCACGATGATGGTAGGTGCATTCCAGCTTCAGCAGCCTGTGAGAAGGGAAAGGAAATAAGTGATTCTAAGTGTGAGATGAATCAGGAAGAGCGCAGAGGTGAACTGAACTGTGTTGAAACTTCCAAAAGTGCATGTCCTGAGCTTAGTGCGAAGTTGATTAAGTTTCAAAACGGAATGTTAGGTTGCCCATATGACTTTGAAGAGATCTGTTCCAAGTTTGTGACCAATGATTCAAACAAGGATTTGATACAGGAAATAGGTCTTATAGGTTCACAAGGAGTTCCATTGTTTGCCCCACCTTCTCCTTATCTGAGTGATTCTACGCTAGCGATACTATCTCAGAAAGATGTCAGTGCACCTGGTCATGGACTGGAGGCGGCTGAACAGCTAGTTTCACAAGGAAAGACTAATGTTGATATTGCATGCAGTAGCAATCTATCCAGGACAGGTGATGGAATGATGTTGCTAAATGCTAACAAACTAGCTTTGGGATTTCTGGAACGAGGGAAGAAAAGACATTCAAATAGCCATTCTTCTGTTGTGGGGGCTGGTTGTTTCTGTGTGGTCCCCCAATCTTCTTTGAGGCCCTTGGTTGGCAAAGTTTGTCAAATTTATAGGAGACTCAAGATCAATCTGCTTGATATTGATGCCTCACTCGCTGAAGAAGCTCTAAGACCATCAAAGGCACAATTGGAAAGGAGATGGGCCTGGCGTGTGTTTGTTAAATCAGCTGTGACAATATATGAAATGGTCCAGGCGATGATTGTGCTGGAGGACATGATAAAGACCGAGTATCTAAGGAATGAGTGGTGGTATTGGTCATCATTTTCAGCTGCTGCCAAAATTTCTACTATGTCTGCACTTGCCCTGCGTATATACTCCCTTGATGCTGCTATCCTGTATGAGAAGATGTATCCAAGTTCAGATCTCACCGACAAGTTGGAGCCCAGCAGCGCACTGGACCTGAAGCTGCTTCCAGTTTTGGATTCAGCAGAAAAAACAAGGTTAACTAGGAAATCTAACAAGAAAAGAAAGGATACAGAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0001763 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004402 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005911 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008080 GO:0008150 GO:0008152 GO:0008327 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009648 GO:0009653 GO:0009791 GO:0009889 GO:0009987 GO:0010016 GO:0010216 GO:0010223 GO:0010228 GO:0010346 GO:0010468 GO:0010556 GO:0016043 GO:0016407 GO:0016410 GO:0016567 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022414 GO:0030054 GO:0031323 GO:0031326 GO:0031935 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0034212 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043565 GO:0043966 GO:0043967 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048367 GO:0048573 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051128 GO:0051171 GO:0051252 GO:0051276 GO:0055044 GO:0060255 GO:0060968 GO:0061458 GO:0061630 GO:0061659 GO:0061733 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0097159 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905393 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromodomain DDT FYRC MBD PHD WHIM1 WSD

Protein Analysis

2231

Amino Acids

245.79

Weight (kDa)

5.55

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 75 - 120 7.4e-08 PHD-finger
MBD PF01429 261 - 311 1.2e-07 Methyl-CpG binding domain
FYRN PF05964 402 - 436 4.7e-06 F/Y-rich N-terminus
PHD PF00628 1291 - 1335 2.2e-11 PHD-finger
WHIM1 PF15612 1369 - 1413 5e-11 WSTF, HB1, Itc1p, MBD9 motif 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1302, 1853
AarI CACCTGC 2 cut(s) 3130, 4846
AatII GACGTC 2 cut(s) 144, 450
Acc36I ACCTGC 4 cut(s) 2397, 3130, 4428, 4846
Acc65I GGTACC 1 cut(s) 5550
AccB1I GGYRCC 3 cut(s) 524, 2051, 5550
AccB7I CCANNNNNTGG 2 cut(s) 1451, 5511
AccBSI CCGCTC 1 cut(s) 366
AccI GTMKAC 4 cut(s) 450, 861, 4548, 5587
AccII CGCG 6 cut(s) 252, 285, 305, 315, 471, 2326
AcoI YGGCCR 5 cut(s) 189, 316, 389, 2825, 3537
AcyI GRCGYC 2 cut(s) 141, 447
AfeI AGCGCT 1 cut(s) 1835
AgeI ACCGGT 1 cut(s) 5553
Alw21I GWGCWC 7 cut(s) 871, 1086, 2670, 2952, 4489, 5376, 6001
Alw26I GTCTC 8 cut(s) 975, 3394, 3552, 4100, 4244, 4465, 5082, 6256
Alw44I GTGCAC 2 cut(s) 867, 5997
AlwNI CAGNNNCTG 7 cut(s) 899, 1154, 1916, 3092, 3145, 4463, 6479
Ama87I CYCGRG 2 cut(s) 179, 242
Aor51HI AGCGCT 1 cut(s) 1835
ApaLI GTGCAC 2 cut(s) 867, 5997
ArsI GACNNNNNNTTYG 4 cut(s) 1481, 1513, 3559, 3591
AseI ATTAAT 4 cut(s) 671, 1473, 2811, 3384
AsiGI ACCGGT 1 cut(s) 5553
Asp700I GAANNNNTTC 4 cut(s) 1396, 1660, 3618, 4978
Asp718I GGTACC 1 cut(s) 5550
AspA2I CCTAGG 1 cut(s) 4969
AsuC2I CCSGG 8 cut(s) 180, 181, 201, 461, 492, 736, 3536, 3977
AsuNHI GCTAGC 2 cut(s) 3322, 5968
AvaI CYCGRG 2 cut(s) 179, 242
AvrII CCTAGG 1 cut(s) 4969
AxyI CCTNAGG 4 cut(s) 1545, 1571, 4307, 5283
BaeGI GKGCMC 6 cut(s) 529, 871, 2056, 5215, 5646, 6001
BaeI ACNNNNGTAYC 4 cut(s) 981, 1014, 5542, 5575
BalI TGGCCA 1 cut(s) 2827
BamHI GGATCC 5 cut(s) 215, 3220, 3530, 3593, 3972
BanI GGYRCC 3 cut(s) 524, 2051, 5550
BanII GRGCYC 3 cut(s) 1086, 5376, 6591
BauI CACGAG 1 cut(s) 2901
BbsI GAAGAC 6 cut(s) 887, 995, 1719, 3515, 4066, 4403
Bbv12I GWGCWC 7 cut(s) 871, 1086, 2670, 2952, 4489, 5376, 6001
BbvCI CCTCAGC 1 cut(s) 5297
BceAI ACGGC 2 cut(s) 289, 5131
BciVI GTATCC 4 cut(s) 1526, 4802, 6567, 6673
BcnI CCSGG 8 cut(s) 180, 181, 201, 461, 492, 736, 3536, 3977
BcoDI GTCTC 8 cut(s) 975, 3394, 3552, 4100, 4244, 4465, 5082, 6256
BfmI CTRYAG 5 cut(s) 1293, 1438, 1728, 3447, 4290
BfoI RGCGCY 1 cut(s) 1837
BfuAI ACCTGC 4 cut(s) 2397, 3130, 4428, 4846
BfuI GTATCC 4 cut(s) 1526, 4802, 6567, 6673
BglII AGATCT 4 cut(s) 1284, 2728, 5847, 6568
BlnI CCTAGG 1 cut(s) 4969
BlpI GCTNAGC 2 cut(s) 205, 1830
BmcAI AGTACT 1 cut(s) 2488
BmeT110I CYCGRG 2 cut(s) 179, 242
BmrI ACTGGG 3 cut(s) 524, 5008, 5577
BmtI GCTAGC 2 cut(s) 3326, 5972
BmuI ACTGGG 3 cut(s) 524, 5008, 5577
BoxI GACNNNNGTC 1 cut(s) 3173
BpiI GAAGAC 6 cut(s) 887, 995, 1719, 3515, 4066, 4403
BplI GAGNNNNNCTC 2 cut(s) 4865, 4897
BpmI CTGGAG 7 cut(s) 821, 2586, 2745, 2988, 5477, 6035, 6434
Bpu10I CCTNAGC 2 cut(s) 5297, 5781
Bpu1102I GCTNAGC 2 cut(s) 205, 1830
BpuMI CCSGG 8 cut(s) 180, 181, 201, 461, 492, 736, 3536, 3977
Bsa29I ATCGAT 1 cut(s) 90
BsaAI YACGTR 2 cut(s) 997, 3656
BsaBI GATNNNNATC 2 cut(s) 1278, 2301
BsaHI GRCGYC 2 cut(s) 141, 447
BsaI GGTCTC 1 cut(s) 975
BsaWI WCCGGW 2 cut(s) 1207, 5553
BsaXI ACNNNNNCTCC 2 cut(s) 5450, 5480
Bse118I RCCGGY 1 cut(s) 5553
Bse21I CCTNAGG 4 cut(s) 1545, 1571, 4307, 5283
Bse3DI GCAATG 5 cut(s) 2577, 3289, 3344, 4034, 5593
Bse8I GATNNNNATC 2 cut(s) 1278, 2301
BseCI ATCGAT 1 cut(s) 90
BseJI GATNNNNATC 2 cut(s) 1278, 2301
BseMI GCAATG 5 cut(s) 2577, 3289, 3344, 4034, 5593
BseRI GAGGAG 9 cut(s) 1588, 2238, 2393, 3083, 3746, 3749, 3951, 4822, 5474
BseSI GKGCMC 6 cut(s) 529, 871, 2056, 5215, 5646, 6001
BseX3I CGGCCG 1 cut(s) 316
BseYI CCCAGC 4 cut(s) 587, 1154, 2975, 6590
BsgI GTGCAG 4 cut(s) 1232, 4584, 5297, 6486
Bsh1236I CGCG 6 cut(s) 252, 285, 305, 315, 471, 2326
Bsh1285I CGRYCG 1 cut(s) 319
BshNI GGYRCC 3 cut(s) 524, 2051, 5550
BshTI ACCGGT 1 cut(s) 5553
BshVI ATCGAT 1 cut(s) 90
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 7 cut(s) 871, 1086, 2670, 2952, 4489, 5376, 6001
BsiHKCI CYCGRG 2 cut(s) 179, 242
BslFI GGGAC 5 cut(s) 368, 1171, 3728, 5315, 6194
BsmAI GTCTC 8 cut(s) 975, 3394, 3552, 4100, 4244, 4465, 5082, 6256
BsmFI GGGAC 5 cut(s) 368, 1171, 3728, 5315, 6194
BsmI GAATGC 5 cut(s) 315, 732, 1794, 2546, 5661
Bso31I GGTCTC 1 cut(s) 975
BsoBI CYCGRG 2 cut(s) 179, 242
Bsp1720I GCTNAGC 2 cut(s) 205, 1830
Bsp19I CCATGG 4 cut(s) 942, 2505, 2865, 3540
BspDI ATCGAT 1 cut(s) 90
BspFNI CGCG 6 cut(s) 252, 285, 305, 315, 471, 2326
BspHI TCATGA 3 cut(s) 1270, 1486, 3408
BspMAI CTGCAG 2 cut(s) 1732, 3451
BspMI ACCTGC 4 cut(s) 2397, 3130, 4428, 4846
BspOI GCTAGC 2 cut(s) 3326, 5972
BspQI GCTCTTC 2 cut(s) 5016, 5724
BspT107I GGYRCC 3 cut(s) 524, 2051, 5550
BspTNI GGTCTC 1 cut(s) 975
BsrBI CCGCTC 1 cut(s) 366
BsrDI GCAATG 5 cut(s) 2577, 3289, 3344, 4034, 5593
BsrFI RCCGGY 1 cut(s) 5553
BssAI RCCGGY 1 cut(s) 5553
BssNAI GTATAC 1 cut(s) 862
BssNI GRCGYC 2 cut(s) 141, 447
BssSI CACGAG 1 cut(s) 2901
Bst1107I GTATAC 1 cut(s) 862
Bst2BI CACGAG 1 cut(s) 2901
Bst6I CTCTTC 6 cut(s) 1933, 3060, 4131, 5016, 5724, 5838
BstACI GRCGYC 2 cut(s) 141, 447
BstAPI GCANNNNNTGC 1 cut(s) 4276
BstBAI YACGTR 2 cut(s) 997, 3656
BstDSI CCRYGG 4 cut(s) 942, 2505, 2865, 3540
BstEII GGTNACC 1 cut(s) 2873
BstENI CCTNNNNNAGG 1 cut(s) 4907
BstFNI CGCG 6 cut(s) 252, 285, 305, 315, 471, 2326
BstH2I RGCGCY 1 cut(s) 1837
BstMAI GTCTC 8 cut(s) 975, 3394, 3552, 4100, 4244, 4465, 5082, 6256
BstMCI CGRYCG 1 cut(s) 319
BstNSI RCATGY 7 cut(s) 225, 303, 1031, 1820, 2546, 5778, 6069
BstPAI GACNNNNGTC 1 cut(s) 3173
BstPI GGTNACC 1 cut(s) 2873
BstSFI CTRYAG 5 cut(s) 1293, 1438, 1728, 3447, 4290
BstSLI GKGCMC 6 cut(s) 529, 871, 2056, 5215, 5646, 6001
BstSNI TACGTA 2 cut(s) 997, 3656
BstUI CGCG 6 cut(s) 252, 285, 305, 315, 471, 2326
BstV2I GAAGAC 6 cut(s) 887, 995, 1719, 3515, 4066, 4403
BstXI CCANNNNNNTGG 2 cut(s) 3231, 5455
BstZ17I GTATAC 1 cut(s) 862
BstZI CGGCCG 1 cut(s) 316
Bsu15I ATCGAT 1 cut(s) 90
Bsu36I CCTNAGG 4 cut(s) 1545, 1571, 4307, 5283
BsuI GTATCC 4 cut(s) 1526, 4802, 6567, 6673
BsuTUI ATCGAT 1 cut(s) 90
BtgI CCRYGG 4 cut(s) 942, 2505, 2865, 3540
BtgZI GCGATG 1 cut(s) 6416
BtsI GCAGTG 2 cut(s) 2654, 3317
BveI ACCTGC 4 cut(s) 2397, 3130, 4428, 4846
CaiI CAGNNNCTG 7 cut(s) 899, 1154, 1916, 3092, 3145, 4463, 6479
CciI TCATGA 3 cut(s) 1270, 1486, 3408
CciNI GCGGCCGC 1 cut(s) 316
Cfr10I RCCGGY 1 cut(s) 5553
Cfr9I CCCGGG 1 cut(s) 179
ClaI ATCGAT 1 cut(s) 90
CsiI ACCWGGT 2 cut(s) 1909, 6001
CspAI ACCGGT 1 cut(s) 5553
CspCI CAANNNNNGTGG 2 cut(s) 3862, 3897
DraI TTTAAA 1 cut(s) 1783
EaeI YGGCCR 5 cut(s) 189, 316, 389, 2825, 3537
EagI CGGCCG 1 cut(s) 316
Eam1104I CTCTTC 6 cut(s) 1933, 3060, 4131, 5016, 5724, 5838
EarI CTCTTC 6 cut(s) 1933, 3060, 4131, 5016, 5724, 5838
EciI GGCGGA 1 cut(s) 5034
Ecl136II GAGCTC 2 cut(s) 1084, 5374
EclXI CGGCCG 1 cut(s) 316
Eco105I TACGTA 2 cut(s) 997, 3656
Eco147I AGGCCT 1 cut(s) 3786
Eco24I GRGCYC 3 cut(s) 1086, 5376, 6591
Eco31I GGTCTC 1 cut(s) 975
Eco32I GATATC 3 cut(s) 424, 1704, 4156
Eco47III AGCGCT 1 cut(s) 1835
Eco52I CGGCCG 1 cut(s) 316
Eco53kI GAGCTC 2 cut(s) 1084, 5374
Eco81I CCTNAGG 4 cut(s) 1545, 1571, 4307, 5283
Eco88I CYCGRG 2 cut(s) 179, 242
Eco91I GGTNACC 1 cut(s) 2873
EcoICRI GAGCTC 2 cut(s) 1084, 5374
EcoNI CCTNNNNNAGG 1 cut(s) 4907
EcoO109I RGGNCCY 5 cut(s) 184, 1260, 3293, 4303, 6228
EcoO65I GGTNACC 1 cut(s) 2873
EcoRI GAATTC 3 cut(s) 455, 3496, 4931
EcoRV GATATC 3 cut(s) 424, 1704, 4156
EcoT22I ATGCAT 3 cut(s) 2548, 3289, 4036
EcoT38I GRGCYC 3 cut(s) 1086, 5376, 6591
FalI AAGNNNNNCTT 6 cut(s) 1249, 1281, 1384, 1416, 3606, 3638
FaqI GGGAC 5 cut(s) 368, 1171, 3728, 5315, 6194
FauI CCCGC 3 cut(s) 245, 278, 298
FauNDI CATATG 2 cut(s) 2556, 5833
FblI GTMKAC 4 cut(s) 450, 861, 4548, 5587
FriOI GRGCYC 3 cut(s) 1086, 5376, 6591
GsaI CCCAGC 4 cut(s) 591, 1158, 2979, 6594
GsuI CTGGAG 7 cut(s) 821, 2586, 2745, 2988, 5477, 6035, 6434
HaeII RGCGCY 1 cut(s) 1837
Hin1I GRCGYC 2 cut(s) 141, 447
HincII GTYRAC 5 cut(s) 451, 834, 2637, 3693, 6651
HindII GTYRAC 5 cut(s) 451, 834, 2637, 3693, 6651
HindIII AAGCTT 3 cut(s) 658, 2792, 3758
HpaI GTTAAC 1 cut(s) 6651
Hpy99I CGWCG 9 cut(s) 29, 143, 146, 275, 449, 452, 455, 3438, 4068
HpyCH4IV ACGT 7 cut(s) 141, 258, 447, 996, 1971, 3655, 4066
HpySE526I ACGT 7 cut(s) 141, 258, 447, 996, 1971, 3655, 4066
Hsp92I GRCGYC 2 cut(s) 141, 447
KpnI GGTACC 1 cut(s) 5554
KspAI GTTAAC 1 cut(s) 6651
LguI GCTCTTC 2 cut(s) 5016, 5724
MabI ACCWGGT 2 cut(s) 1909, 6001
MaeII ACGT 7 cut(s) 141, 258, 447, 996, 1971, 3655, 4066
MbiI CCGCTC 1 cut(s) 366
MfeI CAATTG 1 cut(s) 6332
MlsI TGGCCA 1 cut(s) 2827
MluNI TGGCCA 1 cut(s) 2827
MlyI GAGTC 7 cut(s) 748, 1014, 2504, 2876, 5084, 5297, 6258
MmeI TCCRAC 7 cut(s) 555, 2098, 2346, 3157, 3759, 5009, 6564
Mox20I TGGCCA 1 cut(s) 2827
Mph1103I ATGCAT 3 cut(s) 2548, 3289, 4036
MroXI GAANNNNTTC 4 cut(s) 1396, 1660, 3618, 4978
MscI TGGCCA 1 cut(s) 2827
MslI CAYNNNNRTG 5 cut(s) 1032, 2555, 3229, 4047, 5649
Msp20I TGGCCA 1 cut(s) 2827
MspA1I CMGCKG 6 cut(s) 20, 41, 1613, 3590, 6374, 6476
MunI CAATTG 1 cut(s) 6332
Mva1269I GAATGC 5 cut(s) 315, 732, 1794, 2546, 5661
MvnI CGCG 6 cut(s) 252, 285, 305, 315, 471, 2326
NciI CCSGG 8 cut(s) 180, 181, 201, 461, 492, 736, 3536, 3977
NcoI CCATGG 4 cut(s) 942, 2505, 2865, 3540
NdeI CATATG 2 cut(s) 2556, 5833
NheI GCTAGC 2 cut(s) 3322, 5968
NmeAIII GCCGAG 5 cut(s) 63, 88, 465, 1165, 3529
NmuCI GTSAC 6 cut(s) 532, 3315, 4833, 5392, 5863, 6376
NotI GCGGCCGC 1 cut(s) 316
NsiI ATGCAT 3 cut(s) 2548, 3289, 4036
NspI RCATGY 7 cut(s) 225, 303, 1031, 1820, 2546, 5778, 6069
PaeI GCATGC 2 cut(s) 225, 6069
PagI TCATGA 3 cut(s) 1270, 1486, 3408
PaqCI CACCTGC 2 cut(s) 3130, 4846
PceI AGGCCT 1 cut(s) 3786
PciSI GCTCTTC 2 cut(s) 5016, 5724
PctI GAATGC 5 cut(s) 315, 732, 1794, 2546, 5661
PdmI GAANNNNTTC 4 cut(s) 1396, 1660, 3618, 4978
PflFI GACNNNGTC 1 cut(s) 5025
PflMI CCANNNNNTGG 2 cut(s) 1451, 5511
PfoI TCCNGGA 4 cut(s) 459, 734, 3975, 6083
PinAI ACCGGT 1 cut(s) 5553
PleI GAGTC 7 cut(s) 747, 1014, 2504, 2876, 5084, 5297, 6258
PpsI GAGTC 7 cut(s) 747, 1014, 2504, 2876, 5084, 5297, 6258
Ppu21I YACGTR 2 cut(s) 997, 3656
PpuMI RGGWCCY 2 cut(s) 3293, 4303
PshAI GACNNNNGTC 1 cut(s) 3173
PshBI ATTAAT 4 cut(s) 671, 1473, 2811, 3384
PsiI TTATAA 2 cut(s) 1302, 1853
Psp124BI GAGCTC 2 cut(s) 1086, 5376
Psp5II RGGWCCY 2 cut(s) 3293, 4303
PspEI GGTNACC 1 cut(s) 2873
PspFI CCCAGC 4 cut(s) 587, 1154, 2975, 6590
PspPPI RGGWCCY 2 cut(s) 3293, 4303
PstI CTGCAG 2 cut(s) 1732, 3451
PstNI CAGNNNCTG 7 cut(s) 899, 1154, 1916, 3092, 3145, 4463, 6479
PsyI GACNNNGTC 1 cut(s) 5025
PvuII CAGCTG 5 cut(s) 20, 1613, 3590, 6374, 6476
RseI CAYNNNNRTG 5 cut(s) 1032, 2555, 3229, 4047, 5649
SacI GAGCTC 2 cut(s) 1086, 5376
SalI GTCGAC 1 cut(s) 449
SapI GCTCTTC 2 cut(s) 5016, 5724
ScaI AGTACT 1 cut(s) 2488
SchI GAGTC 7 cut(s) 748, 1014, 2504, 2876, 5084, 5297, 6258
SexAI ACCWGGT 2 cut(s) 1909, 6001
SfcI CTRYAG 5 cut(s) 1293, 1438, 1728, 3447, 4290
SgrDI CGTCGACG 1 cut(s) 449
SmaI CCCGGG 1 cut(s) 181
SmiMI CAYNNNNRTG 5 cut(s) 1032, 2555, 3229, 4047, 5649
SnaBI TACGTA 2 cut(s) 997, 3656
SphI GCATGC 2 cut(s) 225, 6069
SseBI AGGCCT 1 cut(s) 3786
SspI AATATT 1 cut(s) 1779
SstI GAGCTC 2 cut(s) 1086, 5376
StuI AGGCCT 1 cut(s) 3786
TaiI ACGT 7 cut(s) 144, 261, 450, 999, 1974, 3658, 4069
TaqII GACCGA 1 cut(s) 6446
TatI WGTACW 3 cut(s) 2486, 3052, 4925
TseFI GTSAC 6 cut(s) 532, 3315, 4833, 5392, 5863, 6376
Tsp45I GTSAC 6 cut(s) 532, 3315, 4833, 5392, 5863, 6376
TspGWI ACGGA 9 cut(s) 67, 579, 1627, 2854, 3391, 4214, 5163, 5639, 5829
TspMI CCCGGG 1 cut(s) 179
Tth111I GACNNNGTC 1 cut(s) 5025
Van91I CCANNNNNTGG 2 cut(s) 1451, 5511
VneI GTGCAC 2 cut(s) 867, 5997
VspI ATTAAT 4 cut(s) 671, 1473, 2811, 3384
XagI CCTNNNNNAGG 1 cut(s) 4907
XbaI TCTAGA 2 cut(s) 1001, 4652
XceI RCATGY 7 cut(s) 225, 303, 1031, 1820, 2546, 5778, 6069
XcmI CCANNNNNNNNNTGG 2 cut(s) 2000, 6598
XmaI CCCGGG 1 cut(s) 179
XmaJI CCTAGG 1 cut(s) 4969
XmiI GTMKAC 4 cut(s) 450, 861, 4548, 5587
XmnI GAANNNNTTC 4 cut(s) 1396, 1660, 3618, 4978
ZraI GACGTC 2 cut(s) 142, 448
ZrmI AGTACT 1 cut(s) 2488
Zsp2I ATGCAT 3 cut(s) 2548, 3289, 4036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.