AT3G02250

fucose metabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002611
Physical Location & Seq
Reverse (-)
423791 .. 427095
3305 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G02250.1

Sequence Viewer

Length: 1539 bp
ATGTGTAAAGCGGAGAAGTTTCTTTATCACAGGAAACTCTGGGAGATGAAGGTTAAGTTTCTAGGAGATAGTAAGATTGACAAGCTTAAGAACTCCTTGGTTTTGAGGTCTCGTATGAGCTTATGGATGATTCGTGCTATGACTATATTGTTGCTATGGAGTTGCTTTGTTCATTTGGTTGCTGTGGGAGAGATGTGGGGACCTAGATTGTTGAAAGGCTGGCCTTCTTGTTTCAATCACCATGACTTCCCCATTGCTGCAGAAATGACTTCTCTTCCCATGAAAATCGCCCTCCCACCTAAAAGGATATATCAGAACAATGGCTATCTTATGGTTTCTTGCAATGGAGGACTCAACCAAATGCGAGCAGCGATATGTGATATGGTCACTATTGCAAGATACATGAATGTCACACTTATTGTCCCAGAGCTTGACAAGACCTCGTTTTGGAATGATCCAAGTGAGTTCAAAGACATATTCGACGTGGACCATTTCATAAGTTCTTTAAGGGACGAGGTTCGTATACTAAAAGAGCTTCCTCCAAGGCTTAAGAGAAGGGTTAGGCTCGGGCTGTACCACACCATGCCTCCTATTAGCTGGTCCAACATGTCCTACTACCAAGACCAGATTCTTCCGCTGGTGAAGAAGTATAAGGTGGTACATCTGAACAAGACCGATACTCGACTTGCTAATAACGAACTACCTGTTGAGATTCAGAAGCTGCGATGCCGAGCAAATTTCAATGGGCTTAGGTTTACTCCAAAGATTGAGGAATTGGGTAGAAGAGTAGTCAAGATTCTCAGGGAGAAAGGTCCCTTTCTAGTTCTGCATCTCAGATACGAAATGGATATGTTGGCATTTTCTGGTTGCTCACATGGTTGCAACCGCTACGAAGAGGAAGAACTAACAAGAATGAGATATGCTTATCCGTGGTGGAAAGAGAAAGTAATAGACTCCGAGTTGAAGAGGAAAGAAGGTCTTTGCCCATTGACTCCTGAAGAAACAGCTCTTACGCTATCTGCATTGGGTATTGACCGAAATGTTCAGATTTACATAGCTGCAGGAGAAATATACGGTGGTAAAAGGCGGTTAAAGGCTTTAACAGATGTTTTTCCAAATGTGGTGAGGAAAGAAACTCTACTCGATTCTTCTGATTTGAGTTTTTGTAAAAACCATTCTTCTCAAATGGCTGCTCTTGATTACCTTATCTCTCTCGAAAGTGATATATTCGTTCCTACTTATTACGGAAACATGGCGAAAGTCGTGGAAGGTCATCGCAGGTTCTTGGGGTTCAAGAAGACAATTGAGCTAAACCGGAAGTTGTTGGTTAAGCTGATAGATGAATATTATGAAGGATTGTTGAGCTGGGAAGTGTTTTCAACCACGGTTAAGGCCTTTCACGCTACACGAATGGGAGGGCCAAAGAGACGGCTAGTGATTCCAAATAAACCGAAAGAAGAAGACTACTTCTATGCTAATCCATATGAATGTCTTCAGCTATTACATGAGAATAATGGCAATTCACTAGAAGAAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

512

Amino Acids

59.73

Weight (kDa)

9.23

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 109 - 428 1e-106 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1269
AccI GTMKAC 1 cut(s) 523
AciI CCGC 4 cut(s) 11, 635, 886, 1087
AclWI GGATC 1 cut(s) 449
AcsI RAATTY 1 cut(s) 736
AcuI CTGAAG 2 cut(s) 1017, 1478
AfaI GTAC 2 cut(s) 575, 660
AfiI CCNNNNNNNGG 1 cut(s) 447
AflII CTTAAG 2 cut(s) 86, 548
AflIII ACRYGT 1 cut(s) 606
AgsI TTSAA 7 cut(s) 214, 235, 469, 742, 964, 1294, 1380
AjiI CACGTC 1 cut(s) 484
AjuI GAANNNNNNNTTGG 2 cut(s) 612, 644
Alw26I GTCTC 2 cut(s) 114, 1420
AlwI GGATC 1 cut(s) 449
AlwNI CAGNNNCTG 1 cut(s) 721
Ama87I CYCGRG 1 cut(s) 566
AoxI GGCC 3 cut(s) 221, 1392, 1418
ApeKI GCWGC 5 cut(s) 257, 368, 721, 1058, 1190
ApoI RAATTY 1 cut(s) 736
Asp700I GAANNNNTTC 1 cut(s) 1491
AspS9I GGNCC 5 cut(s) 200, 487, 600, 812, 1418
AsuHPI GGTGA 3 cut(s) 230, 652, 1135
AvaI CYCGRG 1 cut(s) 566
AvaII GGWCC 4 cut(s) 200, 487, 600, 812
BbsI GAAGAC 3 cut(s) 1304, 1467, 1484
BbvI GCAGC 5 cut(s) 244, 380, 708, 1045, 1177
BceAI ACGGC 1 cut(s) 1445
BcoDI GTCTC 2 cut(s) 114, 1420
BfaI CTAG 5 cut(s) 62, 204, 821, 1433, 1526
BfmI CTRYAG 2 cut(s) 258, 1059
BfrI CTTAAG 2 cut(s) 86, 548
BfuAI ACCTGC 1 cut(s) 1269
BisI GCNGC 5 cut(s) 258, 369, 722, 1059, 1191
BlsI GCNGC 5 cut(s) 259, 370, 723, 1060, 1192
Bme18I GGWCC 4 cut(s) 200, 487, 600, 812
BmeT110I CYCGRG 1 cut(s) 566
BmgBI CACGTC 1 cut(s) 484
BmgT120I GGNCC 5 cut(s) 200, 487, 600, 812, 1418
BmiI GGNNCC 2 cut(s) 201, 814
BmsI GCATC 2 cut(s) 716, 838
BpiI GAAGAC 3 cut(s) 1304, 1467, 1484
Bpu10I CCTNAGC 1 cut(s) 749
BsaI GGTCTC 1 cut(s) 114
BsaJI CCNNGG 4 cut(s) 96, 542, 929, 1383
BsaWI WCCGGW 1 cut(s) 1314
BsaXI ACNNNNNCTCC 2 cut(s) 571, 601
Bsc4I CCNNNNNNNGG 1 cut(s) 447
Bse3DI GCAATG 2 cut(s) 252, 349
BseDI CCNNGG 4 cut(s) 96, 542, 929, 1383
BseGI GGATG 1 cut(s) 132
BseLI CCNNNNNNNGG 1 cut(s) 447
BseMI GCAATG 2 cut(s) 252, 349
BseMII CTCAG 2 cut(s) 814, 847
BseXI GCAGC 5 cut(s) 244, 380, 708, 1045, 1177
BseYI CCCAGC 1 cut(s) 1365
BshFI GGCC 3 cut(s) 223, 1394, 1420
BsiHKCI CYCGRG 1 cut(s) 566
BsiSI CCGG 1 cut(s) 1315
BslFI GGGAC 4 cut(s) 213, 407, 524, 798
BslI CCNNNNNNNGG 1 cut(s) 447
BsmAI GTCTC 2 cut(s) 114, 1420
BsmBI CGTCTC 1 cut(s) 1420
BsmFI GGGAC 4 cut(s) 213, 407, 524, 798
BsnI GGCC 3 cut(s) 223, 1394, 1420
Bso31I GGTCTC 1 cut(s) 114
BsoBI CYCGRG 1 cut(s) 566
Bsp143I GATC 1 cut(s) 454
BspACI CCGC 4 cut(s) 11, 635, 886, 1087
BspANI GGCC 3 cut(s) 223, 1394, 1420
BspCNI CTCAG 2 cut(s) 813, 846
BspLI GGNNCC 2 cut(s) 201, 814
BspMAI CTGCAG 2 cut(s) 262, 1063
BspMI ACCTGC 1 cut(s) 1269
BspPI GGATC 1 cut(s) 449
BspTI CTTAAG 2 cut(s) 86, 548
BspTNI GGTCTC 1 cut(s) 114
BsrDI GCAATG 2 cut(s) 252, 349
BssECI CCNNGG 4 cut(s) 96, 542, 929, 1383
BssMI GATC 1 cut(s) 454
BssNAI GTATAC 1 cut(s) 524
BssT1I CCWWGG 2 cut(s) 96, 542
Bst1107I GTATAC 1 cut(s) 524
Bst4CI ACNGT 2 cut(s) 1076, 1387
Bst6I CTCTTC 4 cut(s) 279, 778, 888, 959
BstAFI CTTAAG 2 cut(s) 86, 548
BstC8I GCNNGC 2 cut(s) 221, 366
BstDEI CTNAG 3 cut(s) 749, 800, 833
BstDSI CCRYGG 2 cut(s) 929, 1383
BstF5I GGATG 1 cut(s) 132
BstKTI GATC 1 cut(s) 457
BstMAI GTCTC 2 cut(s) 114, 1420
BstMBI GATC 1 cut(s) 454
BstMWI GCNNNNNNNGC 1 cut(s) 1400
BstNSI RCATGY 1 cut(s) 610
BstSFI CTRYAG 2 cut(s) 258, 1059
BstV1I GCAGC 5 cut(s) 244, 380, 708, 1045, 1177
BstV2I GAAGAC 3 cut(s) 1304, 1467, 1484
BstZ17I GTATAC 1 cut(s) 524
BsuRI GGCC 3 cut(s) 223, 1394, 1420
BtgI CCRYGG 2 cut(s) 929, 1383
BtgZI GCGATG 2 cut(s) 739, 1259
BtrI CACGTC 1 cut(s) 484
BtsCI GGATG 1 cut(s) 132
BveI ACCTGC 1 cut(s) 1269
Cac8I GCNNGC 2 cut(s) 221, 366
CaiI CAGNNNCTG 1 cut(s) 721
Cfr13I GGNCC 5 cut(s) 200, 487, 600, 812, 1418
Csp6I GTAC 2 cut(s) 574, 659
CviAII CATG 8 cut(s) 242, 280, 403, 583, 607, 875, 1252, 1505
CviQI GTAC 2 cut(s) 574, 659
DdeI CTNAG 3 cut(s) 749, 800, 833
DpnI GATC 1 cut(s) 456
DpnII GATC 1 cut(s) 454
Eam1104I CTCTTC 4 cut(s) 279, 778, 888, 959
EarI CTCTTC 4 cut(s) 279, 778, 888, 959
Eco130I CCWWGG 2 cut(s) 96, 542
Eco147I AGGCCT 1 cut(s) 1394
Eco31I GGTCTC 1 cut(s) 114
Eco47I GGWCC 4 cut(s) 200, 487, 600, 812
Eco57I CTGAAG 2 cut(s) 1017, 1478
Eco88I CYCGRG 1 cut(s) 566
EcoO109I RGGNCCY 2 cut(s) 200, 812
EcoT14I CCWWGG 2 cut(s) 96, 542
ErhI CCWWGG 2 cut(s) 96, 542
Esp3I CGTCTC 1 cut(s) 1420
FaeI CATG 8 cut(s) 245, 283, 406, 586, 610, 878, 1255, 1508
FalI AAGNNNNNCTT 4 cut(s) 80, 112, 963, 995
FaqI GGGAC 4 cut(s) 213, 407, 524, 798
FatI CATG 8 cut(s) 241, 279, 402, 582, 606, 874, 1251, 1504
FauNDI CATATG 1 cut(s) 1483
FblI GTMKAC 1 cut(s) 523
Fnu4HI GCNGC 5 cut(s) 258, 369, 722, 1059, 1191
FokI GGATG 1 cut(s) 139
Fsp4HI GCNGC 5 cut(s) 258, 369, 722, 1059, 1191
FspBI CTAG 5 cut(s) 62, 204, 821, 1433, 1526
GluI GCNGC 5 cut(s) 258, 369, 722, 1059, 1191
GsaI CCCAGC 1 cut(s) 1369
HaeIII GGCC 3 cut(s) 223, 1394, 1420
HapII CCGG 1 cut(s) 1315
Hin1II CATG 8 cut(s) 245, 283, 406, 586, 610, 878, 1255, 1508
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 9 cut(s) 130, 351, 628, 712, 796, 953, 991, 1145, 1438
HpaII CCGG 1 cut(s) 1315
HphI GGTGA 3 cut(s) 230, 652, 1135
Hpy166II GTNNAC 3 cut(s) 487, 524, 756
Hpy188I TCNGA 7 cut(s) 315, 666, 717, 836, 958, 1047, 1153
Hpy188III TCNNGA 5 cut(s) 793, 995, 1196, 1214, 1294
Hpy8I GTNNAC 3 cut(s) 487, 524, 756
Hpy99I CGWCG 1 cut(s) 485
HpyAV CCTTC 6 cut(s) 43, 234, 549, 968, 1262, 1346
HpyCH4III ACNGT 2 cut(s) 1076, 1387
HpyCH4IV ACGT 1 cut(s) 483
HpyCH4V TGCA 7 cut(s) 260, 342, 395, 829, 882, 1022, 1061
HpyF10VI GCNNNNNNNGC 1 cut(s) 1400
HpyF3I CTNAG 3 cut(s) 749, 800, 833
HpySE526I ACGT 1 cut(s) 483
Hsp92II CATG 8 cut(s) 245, 283, 406, 586, 610, 878, 1255, 1508
Kzo9I GATC 1 cut(s) 454
Lsp1109I GCAGC 5 cut(s) 244, 380, 708, 1045, 1177
LweI GCATC 2 cut(s) 716, 838
MaeI CTAG 5 cut(s) 62, 204, 821, 1433, 1526
MaeII ACGT 1 cut(s) 483
MaeIII GTNAC 2 cut(s) 385, 409
MalI GATC 1 cut(s) 456
MboI GATC 1 cut(s) 454
MfeI CAATTG 1 cut(s) 1302
MluCI AATT 4 cut(s) 736, 773, 1302, 1519
MlyI GAGTC 3 cut(s) 345, 947, 985
MmeI TCCRAC 1 cut(s) 627
MroXI GAANNNNTTC 1 cut(s) 1491
MseI TTAA 8 cut(s) 54, 87, 506, 549, 1091, 1100, 1329, 1389
MslI CAYNNNNRTG 1 cut(s) 1486
MspA1I CMGCKG 1 cut(s) 637
MspCI CTTAAG 2 cut(s) 86, 548
MspI CCGG 1 cut(s) 1315
MunI CAATTG 1 cut(s) 1302
MwoI GCNNNNNNNGC 1 cut(s) 1400
NdeI CATATG 1 cut(s) 1483
NdeII GATC 1 cut(s) 454
NlaIII CATG 8 cut(s) 245, 283, 406, 586, 610, 878, 1255, 1508
NlaIV GGNNCC 2 cut(s) 201, 814
NmeAIII GCCGAG 1 cut(s) 755
NmuCI GTSAC 2 cut(s) 385, 409
NspI RCATGY 1 cut(s) 610
PceI AGGCCT 1 cut(s) 1394
PciI ACATGT 1 cut(s) 606
PdmI GAANNNNTTC 1 cut(s) 1491
PfeI GAWTC 6 cut(s) 130, 628, 712, 796, 1145, 1438
PkrI GCNGC 5 cut(s) 259, 370, 723, 1060, 1192
PleI GAGTC 3 cut(s) 345, 947, 985
PpsI GAGTC 3 cut(s) 345, 947, 985
PpuMI RGGWCCY 2 cut(s) 200, 812
PscI ACATGT 1 cut(s) 606
Psp5II RGGWCCY 2 cut(s) 200, 812
PspFI CCCAGC 1 cut(s) 1365
PspN4I GGNNCC 2 cut(s) 201, 814
PspPI GGNCC 5 cut(s) 200, 487, 600, 812, 1418
PspPPI RGGWCCY 2 cut(s) 200, 812
PstI CTGCAG 2 cut(s) 262, 1063
PstNI CAGNNNCTG 1 cut(s) 721
RsaI GTAC 2 cut(s) 575, 660
RsaNI GTAC 2 cut(s) 574, 659
RseI CAYNNNNRTG 1 cut(s) 1486
SaqAI TTAA 8 cut(s) 54, 87, 506, 549, 1091, 1100, 1329, 1389
SatI GCNGC 5 cut(s) 258, 369, 722, 1059, 1191
Sau3AI GATC 1 cut(s) 454
Sau96I GGNCC 5 cut(s) 200, 487, 600, 812, 1418
SchI GAGTC 3 cut(s) 345, 947, 985
SfaNI GCATC 2 cut(s) 716, 838
SfcI CTRYAG 2 cut(s) 258, 1059
SinI GGWCC 4 cut(s) 200, 487, 600, 812
SmiMI CAYNNNNRTG 1 cut(s) 1486
SmlI CTYRAG 2 cut(s) 86, 548
SmoI CTYRAG 2 cut(s) 86, 548
Sse9I AATT 4 cut(s) 736, 773, 1302, 1519
SseBI AGGCCT 1 cut(s) 1394
SsiI CCGC 4 cut(s) 11, 635, 886, 1087
SspI AATATT 1 cut(s) 1346
SspMI CTAG 5 cut(s) 62, 204, 821, 1433, 1526
StuI AGGCCT 1 cut(s) 1394
StyI CCWWGG 2 cut(s) 96, 542
TaaI ACNGT 2 cut(s) 1076, 1387
TaiI ACGT 1 cut(s) 486
TaqI TCGA 4 cut(s) 480, 682, 1143, 1215
TaqII GACCGA 2 cut(s) 689, 1050
TasI AATT 4 cut(s) 736, 773, 1302, 1519
TfiI GAWTC 6 cut(s) 130, 628, 712, 796, 1145, 1438
Tru1I TTAA 8 cut(s) 54, 87, 506, 549, 1091, 1100, 1329, 1389
Tru9I TTAA 8 cut(s) 54, 87, 506, 549, 1091, 1100, 1329, 1389
TseFI GTSAC 2 cut(s) 385, 409
TseI GCWGC 5 cut(s) 257, 368, 721, 1058, 1190
Tsp45I GTSAC 2 cut(s) 385, 409
TspDTI ATGAA 8 cut(s) 62, 161, 296, 419, 484, 1356, 1365, 1500
TspGWI ACGGA 2 cut(s) 918, 1260
Vha464I CTTAAG 2 cut(s) 86, 548
VpaK11BI GGWCC 4 cut(s) 200, 487, 600, 812
XapI RAATTY 1 cut(s) 736
XceI RCATGY 1 cut(s) 610
XmiI GTMKAC 1 cut(s) 523
XmnI GAANNNNTTC 1 cut(s) 1491
XspI CTAG 5 cut(s) 62, 204, 821, 1433, 1526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.