FvH4_6g46300

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002611
Physical Location & Seq
Reverse (-)
35337541 .. 35340895
3355 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g46300.t1

Sequence Viewer

Length: 1503 bp
ATGTGTAAAGCAGGGGACTTTGAGAGGTGCGATTACAGAACAGAGTGGAAGATGGGGTACAAGGTTTTGAGTAATGTTGAGAAATTGAAGAACTCTGTTCTGAGGATGAACTCATGGTTGTTGATCATGAGGGCAATTGTGACAGCTTTGGTTTGGGCCTCTGCTGTTCAGTTGATTGGTATTTGGTTGATTAGAGGTCCAAGAGTGTTCAAGGTTTGGCCTCCTTGTAATCAGTTAGACTTGCATGTTGCTTCTGAATTGCCTAGGCTTCCAATCCCAGTTTTCCCACCAAAGAGGGTATACAAGAATAATGGATATCTGATGGTTTCTTGCAATGGAGGATTGAATCAAATGCGAGCTGCGATTTGTGATATGGTTGCGATTGCCAGATACATGAATGTGACTCTTATTGTTCCTGAGTTGGATAAGAACTCGTTTTGGGCTGCAAGTGAGTTTCAAGACATTTTCGATGTCAATCATTTCATTAGATCATTGAGAGATGAGGTTCGGATACTAAAGCAACTGCCCCCAAAGCTCAACAACAGACCTGCATACTCATTGCCACCTGTTAGTTGGTCCAACATTTCCTACTACCTTCATCAGATCCTACCGCTGGTACGGAAGTATAAAGTAGTGCATTTGAACAAAACTGATGCTCGAGTGGCTAACAATGGGCTACCTCTTGAGATTCAGAAGCTGAGATGTAGAGTCAATTTTCATGGTCTGAGATTTACTTCCCAGATAGAGGAAACCGGCCGAAAGCTTGTCAGAATTTTGAGAGAAAAAGGCCCTTTTCTGGTGCTTCATCTCAGATATGAAATGGACATGTTGTCATTCTCTGGCTGCAGCCATGGTTGCAACAGTGATGAGGTGGAAGAACTCAACACAATGAGATATGCTAATCCATTGTGGAAGGAGAAAGACATTGATTCCGAATTGAAAAGGGTAGAAGGCTTGTGCCCTCTAACACCAGAGGAAACTACTCTAGTATTGGTTGCATTAGGTATAGATCACAATGTTCAGATTTACATTGCTGCCGGAGAAATATACGGTGGAGATAGAAGGCTGGAAGGTCTGAAATCTGCTTTTCCTAATGTGGTCAGGAAAGAGACGCTGCTCGATCCTTCAGACTTGAGGTTCTTCCCAAACCACTCTTCACAAATGGCAGCATTGGACTACCTTGTGTCCCTTGAGAGTGACATTTTCGTCCCAACATACGATGGAAACATGGCCAAAGTTGTTGAAGGCCATCGCAGGTTCTTAGGTTTCAAAAAGACAATTTTACTAAACAGAAGGCTTCTCATTGGTTTGATAGATCAGTTCACTAGTGGATCATTGAGCTGGGATGAGTTCTCCTCCATTGTGAAGGAAGCTCACGCTGATCGAATGGGGAGCCCTAAAAAAAGAGTTTCTATTGTTGATAAACCAAAAGAAGAAGATTACTTCTACTCCAATCCACATGAATGCCTGCAACTGTTAGATGAGCCACTGAGAACTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

501

Amino Acids

57.47

Weight (kDa)

8.91

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 106 - 420 9e-103 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1205
Acc36I ACCTGC 2 cut(s) 556, 1245
AccI GTMKAC 1 cut(s) 300
AciI CCGC 1 cut(s) 611
AclWI GGATC 3 cut(s) 598, 1115, 1339
AcoI YGGCCR 2 cut(s) 754, 1230
AcsI RAATTY 1 cut(s) 771
AcuI CTGAAG 1 cut(s) 1110
AfaI GTAC 2 cut(s) 59, 618
AfiI CCNNNNNNNGG 3 cut(s) 613, 745, 796
AflIII ACRYGT 1 cut(s) 825
AgsI TTSAA 8 cut(s) 88, 211, 346, 458, 643, 940, 1244, 1270
AhdI GACNNNNNGTC 1 cut(s) 829
AhlI ACTAGT 1 cut(s) 1325
AjuI GAANNNNNNNTTGG 2 cut(s) 1138, 1170
AluBI AGCT 7 cut(s) 146, 359, 535, 697, 763, 1341, 1373
AluI AGCT 7 cut(s) 146, 359, 535, 697, 763, 1341, 1373
Alw26I GTCTC 1 cut(s) 1103
AlwI GGATC 3 cut(s) 598, 1115, 1339
AlwNI CAGNNNCTG 1 cut(s) 697
Ama87I CYCGRG 1 cut(s) 657
AoxI GGCC 6 cut(s) 156, 218, 754, 787, 1230, 1246
ApeKI GCWGC 7 cut(s) 359, 443, 843, 846, 1034, 1114, 1166
ApoI RAATTY 1 cut(s) 771
AspA2I CCTAGG 1 cut(s) 263
AspS9I GGNCC 4 cut(s) 156, 197, 576, 788
AvaI CYCGRG 1 cut(s) 657
AvaII GGWCC 2 cut(s) 197, 576
AvrII CCTAGG 1 cut(s) 263
BaeGI GKGCMC 1 cut(s) 962
BaeI ACNNNNGTAYC 2 cut(s) 608, 641
BalI TGGCCA 1 cut(s) 1232
BanII GRGCYC 1 cut(s) 1397
BarI GAAGNNNNNNTAC 2 cut(s) 41, 73
BbvI GCAGC 7 cut(s) 346, 430, 830, 858, 1021, 1101, 1178
BccI CCATC 4 cut(s) 46, 316, 1214, 1257
BciVI GTATCC 1 cut(s) 504
BclI TGATCA 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 1103
BcuI ACTAGT 1 cut(s) 1325
BfaI CTAG 3 cut(s) 264, 986, 1326
BfmI CTRYAG 1 cut(s) 844
BfuAI ACCTGC 2 cut(s) 556, 1245
BfuI GTATCC 1 cut(s) 504
BisI GCNGC 7 cut(s) 360, 444, 844, 847, 1035, 1115, 1167
BlnI CCTAGG 1 cut(s) 263
BlsI GCNGC 7 cut(s) 361, 445, 845, 848, 1036, 1116, 1168
Bme18I GGWCC 2 cut(s) 197, 576
BmeRI GACNNNNNGTC 1 cut(s) 829
BmeT110I CYCGRG 1 cut(s) 657
BmgT120I GGNCC 4 cut(s) 156, 197, 576, 788
BmiI GGNNCC 1 cut(s) 1394
BmrI ACTGGG 1 cut(s) 272
BmsI GCATC 1 cut(s) 643
BmuI ACTGGG 1 cut(s) 272
BplI GAGNNNNNCTC 2 cut(s) 1340, 1372
BpuEI CTTGAG 3 cut(s) 704, 1153, 1211
BsaBI GATNNNNATC 1 cut(s) 474
BsaJI CCNNGG 2 cut(s) 263, 850
BsaXI ACNNNNNCTCC 2 cut(s) 1433, 1463
Bsc4I CCNNNNNNNGG 3 cut(s) 613, 745, 796
Bse118I RCCGGY 1 cut(s) 752
Bse1I ACTGG 1 cut(s) 278
Bse3DI GCAATG 3 cut(s) 340, 557, 1029
Bse8I GATNNNNATC 1 cut(s) 474
BseDI CCNNGG 2 cut(s) 263, 850
BseGI GGATG 2 cut(s) 111, 1351
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 3 cut(s) 613, 745, 796
BseMI GCAATG 3 cut(s) 340, 557, 1029
BseMII CTCAG 6 cut(s) 92, 408, 689, 716, 823, 1481
BseNI ACTGG 1 cut(s) 278
BseRI GAGGAG 1 cut(s) 1345
BseSI GKGCMC 1 cut(s) 962
BseX3I CGGCCG 1 cut(s) 754
BseXI GCAGC 7 cut(s) 346, 430, 830, 858, 1021, 1101, 1178
BseYI CCCAGC 1 cut(s) 1341
Bsh1285I CGRYCG 1 cut(s) 757
BshFI GGCC 6 cut(s) 158, 220, 756, 789, 1232, 1248
BsiEI CGRYCG 1 cut(s) 757
BsiHKCI CYCGRG 1 cut(s) 657
BsiSI CCGG 2 cut(s) 753, 1038
BslFI GGGAC 3 cut(s) 29, 1171, 1193
BslI CCNNNNNNNGG 3 cut(s) 613, 745, 796
BsmAI GTCTC 1 cut(s) 1103
BsmBI CGTCTC 1 cut(s) 1103
BsmFI GGGAC 3 cut(s) 29, 1171, 1193
BsmI GAATGC 1 cut(s) 1469
BsnI GGCC 6 cut(s) 158, 220, 756, 789, 1232, 1248
BsoBI CYCGRG 1 cut(s) 657
Bsp1286I GDGCHC 2 cut(s) 962, 1397
Bsp143I GATC 8 cut(s) 123, 488, 603, 1009, 1120, 1315, 1331, 1381
Bsp19I CCATGG 1 cut(s) 850
BspACI CCGC 1 cut(s) 611
BspANI GGCC 6 cut(s) 158, 220, 756, 789, 1232, 1248
BspCNI CTCAG 6 cut(s) 93, 409, 690, 717, 822, 1482
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 1 cut(s) 1394
BspMAI CTGCAG 1 cut(s) 848
BspMI ACCTGC 2 cut(s) 556, 1245
BspPI GGATC 3 cut(s) 598, 1115, 1339
BsrDI GCAATG 3 cut(s) 340, 557, 1029
BsrFI RCCGGY 1 cut(s) 752
BsrI ACTGG 1 cut(s) 278
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 2 cut(s) 263, 850
BssMI GATC 8 cut(s) 123, 488, 603, 1009, 1120, 1315, 1331, 1381
BssNAI GTATAC 1 cut(s) 301
BssT1I CCWWGG 2 cut(s) 263, 850
Bst1107I GTATAC 1 cut(s) 301
Bst4CI ACNGT 3 cut(s) 863, 1052, 1476
Bst6I CTCTTC 1 cut(s) 1159
BstC8I GCNNGC 2 cut(s) 357, 1469
BstDEI CTNAG 7 cut(s) 101, 417, 698, 725, 809, 1261, 1490
BstDSI CCRYGG 1 cut(s) 850
BstF5I GGATG 2 cut(s) 111, 1351
BstKTI GATC 8 cut(s) 126, 491, 606, 1012, 1123, 1318, 1334, 1384
BstMAI GTCTC 1 cut(s) 1103
BstMBI GATC 8 cut(s) 123, 488, 603, 1009, 1120, 1315, 1331, 1381
BstMCI CGRYCG 1 cut(s) 757
BstMWI GCNNNNNNNGC 3 cut(s) 532, 662, 855
BstNSI RCATGY 2 cut(s) 248, 829
BstSFI CTRYAG 1 cut(s) 844
BstSLI GKGCMC 1 cut(s) 962
BstV1I GCAGC 7 cut(s) 346, 430, 830, 858, 1021, 1101, 1178
BstX2I RGATCY 1 cut(s) 603
BstYI RGATCY 1 cut(s) 603
BstZ17I GTATAC 1 cut(s) 301
BstZI CGGCCG 1 cut(s) 754
BsuI GTATCC 1 cut(s) 504
BsuRI GGCC 6 cut(s) 158, 220, 756, 789, 1232, 1248
BtgI CCRYGG 1 cut(s) 850
BtgZI GCGATG 1 cut(s) 1235
BtsCI GGATG 2 cut(s) 111, 1351
BtsIMutI CAGTG 2 cut(s) 868, 1487
BveI ACCTGC 2 cut(s) 556, 1245
Cac8I GCNNGC 2 cut(s) 357, 1469
CaiI CAGNNNCTG 1 cut(s) 697
CciI TCATGA 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 752
Cfr13I GGNCC 4 cut(s) 156, 197, 576, 788
CseI GACGC 1 cut(s) 1120
Csp6I GTAC 2 cut(s) 58, 617
CviQI GTAC 2 cut(s) 58, 617
DdeI CTNAG 7 cut(s) 101, 417, 698, 725, 809, 1261, 1490
DpnI GATC 8 cut(s) 125, 490, 605, 1011, 1122, 1317, 1333, 1383
DpnII GATC 8 cut(s) 123, 488, 603, 1009, 1120, 1315, 1331, 1381
DrdI GACNNNNNNGTC 1 cut(s) 1205
DriI GACNNNNNGTC 1 cut(s) 829
DseDI GACNNNNNNGTC 1 cut(s) 1205
EaeI YGGCCR 2 cut(s) 754, 1230
EagI CGGCCG 1 cut(s) 754
Eam1104I CTCTTC 1 cut(s) 1159
Eam1105I GACNNNNNGTC 1 cut(s) 829
EarI CTCTTC 1 cut(s) 1159
EclXI CGGCCG 1 cut(s) 754
Eco130I CCWWGG 2 cut(s) 263, 850
Eco24I GRGCYC 1 cut(s) 1397
Eco32I GATATC 1 cut(s) 317
Eco47I GGWCC 2 cut(s) 197, 576
Eco52I CGGCCG 1 cut(s) 754
Eco57I CTGAAG 1 cut(s) 1110
Eco88I CYCGRG 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 788
EcoRV GATATC 1 cut(s) 317
EcoT14I CCWWGG 2 cut(s) 263, 850
EcoT38I GRGCYC 1 cut(s) 1397
ErhI CCWWGG 2 cut(s) 263, 850
Esp3I CGTCTC 1 cut(s) 1103
FaqI GGGAC 3 cut(s) 29, 1171, 1193
FbaI TGATCA 1 cut(s) 123
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 7 cut(s) 360, 444, 844, 847, 1035, 1115, 1167
FokI GGATG 2 cut(s) 118, 1358
FriOI GRGCYC 1 cut(s) 1397
Fsp4HI GCNGC 7 cut(s) 360, 444, 844, 847, 1035, 1115, 1167
FspBI CTAG 3 cut(s) 264, 986, 1326
GluI GCNGC 7 cut(s) 360, 444, 844, 847, 1035, 1115, 1167
GsaI CCCAGC 1 cut(s) 1345
HaeIII GGCC 6 cut(s) 158, 220, 756, 789, 1232, 1248
HapII CCGG 2 cut(s) 753, 1038
HgaI GACGC 1 cut(s) 1120
HindIII AAGCTT 1 cut(s) 761
HinfI GANTC 5 cut(s) 346, 403, 688, 708, 929
HpaII CCGG 2 cut(s) 753, 1038
Hpy166II GTNNAC 2 cut(s) 301, 1323
Hpy188III TCNNGA 5 cut(s) 127, 416, 458, 683, 1102
Hpy8I GTNNAC 2 cut(s) 301, 1323
HpyAV CCTTC 9 cut(s) 605, 907, 944, 1056, 1064, 1134, 1238, 1287, 1360
HpyCH4III ACNGT 3 cut(s) 863, 1052, 1476
HpyCH4V TGCA 9 cut(s) 244, 333, 446, 551, 637, 846, 858, 998, 1471
HpyF10VI GCNNNNNNNGC 3 cut(s) 532, 662, 855
HpyF3I CTNAG 7 cut(s) 101, 417, 698, 725, 809, 1261, 1490
Ksp22I TGATCA 1 cut(s) 123
Kzo9I GATC 8 cut(s) 123, 488, 603, 1009, 1120, 1315, 1331, 1381
LmnI GCTCC 1 cut(s) 1392
Lsp1109I GCAGC 7 cut(s) 346, 430, 830, 858, 1021, 1101, 1178
LweI GCATC 1 cut(s) 643
MaeI CTAG 3 cut(s) 264, 986, 1326
MaeIII GTNAC 3 cut(s) 139, 400, 1196
MalI GATC 8 cut(s) 125, 490, 605, 1011, 1122, 1317, 1333, 1383
MboI GATC 8 cut(s) 123, 488, 603, 1009, 1120, 1315, 1331, 1381
MboII GAAGA 7 cut(s) 61, 100, 887, 1132, 1146, 1445, 1448
MfeI CAATTG 1 cut(s) 135
MflI RGATCY 1 cut(s) 603
MhlI GDGCHC 2 cut(s) 962, 1397
MlsI TGGCCA 1 cut(s) 1232
MluCI AATT 7 cut(s) 83, 135, 257, 712, 771, 935, 1278
MluNI TGGCCA 1 cut(s) 1232
MlyI GAGTC 2 cut(s) 397, 717
MmeI TCCRAC 2 cut(s) 402, 603
Mox20I TGGCCA 1 cut(s) 1232
MscI TGGCCA 1 cut(s) 1232
MslI CAYNNNNRTG 2 cut(s) 398, 1462
Msp20I TGGCCA 1 cut(s) 1232
MspA1I CMGCKG 1 cut(s) 613
MspI CCGG 2 cut(s) 753, 1038
MunI CAATTG 1 cut(s) 135
Mva1269I GAATGC 1 cut(s) 1469
MwoI GCNNNNNNNGC 3 cut(s) 532, 662, 855
NcoI CCATGG 1 cut(s) 850
NdeII GATC 8 cut(s) 123, 488, 603, 1009, 1120, 1315, 1331, 1381
NlaIV GGNNCC 1 cut(s) 1394
NmuCI GTSAC 3 cut(s) 139, 400, 1196
NspI RCATGY 2 cut(s) 248, 829
PaeR7I CTCGAG 1 cut(s) 657
PagI TCATGA 1 cut(s) 126
PciI ACATGT 1 cut(s) 825
PctI GAATGC 1 cut(s) 1469
PfeI GAWTC 3 cut(s) 346, 688, 929
PkrI GCNGC 7 cut(s) 361, 445, 845, 848, 1036, 1116, 1168
PleI GAGTC 2 cut(s) 397, 716
PpsI GAGTC 2 cut(s) 397, 716
PscI ACATGT 1 cut(s) 825
PspFI CCCAGC 1 cut(s) 1341
PspN4I GGNNCC 1 cut(s) 1394
PspPI GGNCC 4 cut(s) 156, 197, 576, 788
PspXI VCTCGAGB 1 cut(s) 657
PstI CTGCAG 1 cut(s) 848
PstNI CAGNNNCTG 1 cut(s) 697
PsuI RGATCY 1 cut(s) 603
RsaI GTAC 2 cut(s) 59, 618
RsaNI GTAC 2 cut(s) 58, 617
RseI CAYNNNNRTG 2 cut(s) 398, 1462
SatI GCNGC 7 cut(s) 360, 444, 844, 847, 1035, 1115, 1167
Sau3AI GATC 8 cut(s) 123, 488, 603, 1009, 1120, 1315, 1331, 1381
Sau96I GGNCC 4 cut(s) 156, 197, 576, 788
SchI GAGTC 2 cut(s) 397, 717
SduI GDGCHC 2 cut(s) 962, 1397
SfaNI GCATC 1 cut(s) 643
SfcI CTRYAG 1 cut(s) 844
Sfr274I CTCGAG 1 cut(s) 657
SinI GGWCC 2 cut(s) 197, 576
SlaI CTCGAG 1 cut(s) 657
SmiMI CAYNNNNRTG 2 cut(s) 398, 1462
SmlI CTYRAG 4 cut(s) 657, 683, 1132, 1190
SmoI CTYRAG 4 cut(s) 657, 683, 1132, 1190
SpeI ACTAGT 1 cut(s) 1325
Sse9I AATT 7 cut(s) 83, 135, 257, 712, 771, 935, 1278
SsiI CCGC 1 cut(s) 611
SspMI CTAG 3 cut(s) 264, 986, 1326
StyI CCWWGG 2 cut(s) 263, 850
TaaI ACNGT 3 cut(s) 863, 1052, 1476
TaqI TCGA 4 cut(s) 468, 658, 1119, 1384
TasI AATT 7 cut(s) 83, 135, 257, 712, 771, 935, 1278
TfiI GAWTC 3 cut(s) 346, 688, 929
TscAI CASTG 2 cut(s) 868, 1494
TseFI GTSAC 3 cut(s) 139, 400, 1196
TseI GCWGC 7 cut(s) 359, 443, 843, 846, 1034, 1114, 1166
Tsp45I GTSAC 3 cut(s) 139, 400, 1196
TspDTI ATGAA 8 cut(s) 122, 410, 472, 587, 707, 794, 831, 1476
TspGWI ACGGA 1 cut(s) 634
TspRI CASTG 2 cut(s) 868, 1494
VpaK11BI GGWCC 2 cut(s) 197, 576
XapI RAATTY 1 cut(s) 771
XceI RCATGY 2 cut(s) 248, 829
XcmI CCANNNNNNNNNTGG 1 cut(s) 570
XhoI CTCGAG 1 cut(s) 657
XmaJI CCTAGG 1 cut(s) 263
XmiI GTMKAC 1 cut(s) 300
XspI CTAG 3 cut(s) 264, 986, 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.