Rh2DG599700

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002611
Physical Location & Seq
Reverse (-)
83161424 .. 83163713
2290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG599700.1

Sequence Viewer

Length: 1131 bp
ATGGTTGCAATTGCCAGATACTTGAATGTGACTCTTATCGTTCCAGAGCTGGATAAGAACTCATTTTGGGCTGCGAGTGAGTTTCAAGACATTTTCGATGTTAATCATTTCATTAGATCATTGAGAGATGAGGTTCGGATACTAAAGAAACTGCCCCCAAAGCTCAACAAGAGAGCAGTATACTCATTGCCACCTGTTAGTTGGTCCAACATTTCCTACTACATTCATCAGATCCTACCACTGGTGCGAAAGTATAAAGTAGTGCATTTGAACAAAACCGATGCTCGAGTGGCTAACAATGGGCTACCTCTTGAGATTCAGAAGTTGAGATGCAGAGTCAATTTCCATGGTCTGAGATTTACTTCCCAGATAGAGGAAACCGGTCGAAAGCTTGTCAGAATTTTAAGAGAAAAAGGGCCTTTTCTAGTGCTTCATCTCAGATATGAAATGGACATGTTGGCATTCTCTGGCTGCAGCCATGGTTGCACCAATGACGAGGTGGAAGAACTCAACACAATGAGATATGCTAATCCATTGTGGAAGGAAAAAGACATTGATTCTGAAATGAAAAGGGTAGAAGGCTTGTGCCCTCTAACACCAGAGGAAACTGCTCTGGTATTGGTTGCATTAGGTATAGATCACAATGTTCAGATTTATATTGCTGCCGGAGACATATACGGTGGAGATCGAAGAATGGAAGGTTTGAAAGCTGCTTTTCCTAATGTGGTCAGGAAAGAGACGCTGCTCGAGCCTTCGGAATTGAGGTTCTTCCCAAACCACTCTTCTCAAATGGCAGCATTGGACTACCTTGTGTCTCTGGAGAGTGACATTTTCGTTCCCACATACGATGGAAACATGGCCAAAGTTGTTGAAGGCCATCGCAGGTTCTTAGGTTTCAAAAAGACTATTTTACTAGACAGAAGGTTTCTCATTGATTTGATAGATCAGTTTACTAGTGGATCATTGAGCTGGGATGAGTTCTCTTCCATTGTGAAGGAAGCTCATGCTGATCGGATGGGGAGCCCGAAAAAAAGAGTTTCTATTGTTGATAAACCAAAAGAAGAAGATTACTTCTACTCAAATCCACATGAATGTTTGCAACTGTTAGATGAGCCATTGAGAACAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

376

Amino Acids

43.36

Weight (kDa)

7.18

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 1 - 296 8e-92 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 873
AccI GTMKAC 1 cut(s) 180
AclWI GGATC 2 cut(s) 226, 967
AcoI YGGCCR 1 cut(s) 858
AcsI RAATTY 1 cut(s) 399
AfiI CCNNNNNNNGG 2 cut(s) 241, 373
AflIII ACRYGT 1 cut(s) 453
AgeI ACCGGT 1 cut(s) 380
AgsI TTSAA 6 cut(s) 25, 86, 271, 706, 872, 898
AhlI ACTAGT 1 cut(s) 953
AjuI GAANNNNNNNTTGG 2 cut(s) 766, 798
AloI GAACNNNNNNTCC 2 cut(s) 749, 781
AluBI AGCT 6 cut(s) 49, 163, 391, 710, 969, 1001
AluI AGCT 6 cut(s) 49, 163, 391, 710, 969, 1001
Alw26I GTCTC 3 cut(s) 663, 731, 819
AlwI GGATC 2 cut(s) 226, 967
Ama87I CYCGRG 2 cut(s) 285, 746
AoxI GGCC 3 cut(s) 416, 858, 874
ApeKI GCWGC 7 cut(s) 71, 471, 474, 662, 710, 742, 794
ApoI RAATTY 1 cut(s) 399
AsiGI ACCGGT 1 cut(s) 380
AspS9I GGNCC 2 cut(s) 204, 416
AvaI CYCGRG 2 cut(s) 285, 746
AvaII GGWCC 1 cut(s) 204
BaeGI GKGCMC 1 cut(s) 590
BalI TGGCCA 1 cut(s) 860
BanII GRGCYC 1 cut(s) 1025
BbvI GCAGC 7 cut(s) 58, 458, 486, 649, 697, 729, 806
BccI CCATC 3 cut(s) 842, 885, 1009
BciVI GTATCC 1 cut(s) 132
BcoDI GTCTC 3 cut(s) 663, 731, 819
BcuI ACTAGT 1 cut(s) 953
BfaI CTAG 3 cut(s) 425, 914, 954
BfmI CTRYAG 1 cut(s) 472
BfuAI ACCTGC 1 cut(s) 873
BfuI GTATCC 1 cut(s) 132
BisI GCNGC 7 cut(s) 72, 472, 475, 663, 711, 743, 795
BlsI GCNGC 7 cut(s) 73, 473, 476, 664, 712, 744, 796
Bme18I GGWCC 1 cut(s) 204
BmeT110I CYCGRG 2 cut(s) 285, 746
BmgT120I GGNCC 2 cut(s) 204, 416
BmiI GGNNCC 1 cut(s) 1022
BmsI GCATC 2 cut(s) 271, 320
BpmI CTGGAG 1 cut(s) 839
BpuEI CTTGAG 1 cut(s) 332
BsaBI GATNNNNATC 1 cut(s) 102
BsaJI CCNNGG 2 cut(s) 346, 478
BsaWI WCCGGW 1 cut(s) 380
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 373
Bse118I RCCGGY 1 cut(s) 380
Bse1I ACTGG 1 cut(s) 246
Bse3DI GCAATG 1 cut(s) 185
Bse8I GATNNNNATC 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 346, 478
BseGI GGATG 2 cut(s) 979, 1020
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 2 cut(s) 241, 373
BseMI GCAATG 1 cut(s) 185
BseMII CTCAG 2 cut(s) 344, 451
BseNI ACTGG 1 cut(s) 246
BseSI GKGCMC 1 cut(s) 590
BseXI GCAGC 7 cut(s) 58, 458, 486, 649, 697, 729, 806
BseYI CCCAGC 1 cut(s) 969
Bsh1285I CGRYCG 1 cut(s) 385
BshFI GGCC 3 cut(s) 418, 860, 876
BshTI ACCGGT 1 cut(s) 380
BsiEI CGRYCG 1 cut(s) 385
BsiHKCI CYCGRG 2 cut(s) 285, 746
BsiSI CCGG 2 cut(s) 381, 666
BslI CCNNNNNNNGG 2 cut(s) 241, 373
BsmAI GTCTC 3 cut(s) 663, 731, 819
BsmBI CGTCTC 1 cut(s) 731
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 3 cut(s) 418, 860, 876
BsoBI CYCGRG 2 cut(s) 285, 746
Bsp1286I GDGCHC 2 cut(s) 590, 1025
Bsp143I GATC 7 cut(s) 116, 231, 637, 685, 943, 959, 1009
Bsp19I CCATGG 2 cut(s) 346, 478
BspANI GGCC 3 cut(s) 418, 860, 876
BspCNI CTCAG 2 cut(s) 345, 450
BspLI GGNNCC 1 cut(s) 1022
BspMAI CTGCAG 1 cut(s) 476
BspMI ACCTGC 1 cut(s) 873
BspPI GGATC 2 cut(s) 226, 967
BsrDI GCAATG 1 cut(s) 185
BsrFI RCCGGY 1 cut(s) 380
BsrI ACTGG 1 cut(s) 246
BssAI RCCGGY 1 cut(s) 380
BssECI CCNNGG 2 cut(s) 346, 478
BssMI GATC 7 cut(s) 116, 231, 637, 685, 943, 959, 1009
BssNAI GTATAC 1 cut(s) 181
BssT1I CCWWGG 2 cut(s) 346, 478
Bst1107I GTATAC 1 cut(s) 181
Bst4CI ACNGT 2 cut(s) 680, 1104
Bst6I CTCTTC 2 cut(s) 787, 988
BstDEI CTNAG 3 cut(s) 353, 437, 889
BstDSI CCRYGG 2 cut(s) 346, 478
BstF5I GGATG 2 cut(s) 979, 1020
BstKTI GATC 7 cut(s) 119, 234, 640, 688, 946, 962, 1012
BstMAI GTCTC 3 cut(s) 663, 731, 819
BstMBI GATC 7 cut(s) 116, 231, 637, 685, 943, 959, 1009
BstMCI CGRYCG 1 cut(s) 385
BstMWI GCNNNNNNNGC 4 cut(s) 160, 290, 483, 748
BstNSI RCATGY 1 cut(s) 457
BstSFI CTRYAG 1 cut(s) 472
BstSLI GKGCMC 1 cut(s) 590
BstV1I GCAGC 7 cut(s) 58, 458, 486, 649, 697, 729, 806
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BstZ17I GTATAC 1 cut(s) 181
BsuI GTATCC 1 cut(s) 132
BsuRI GGCC 3 cut(s) 418, 860, 876
BtgI CCRYGG 2 cut(s) 346, 478
BtgZI GCGATG 1 cut(s) 863
BtsCI GGATG 2 cut(s) 979, 1020
BtsIMutI CAGTG 1 cut(s) 239
BveI ACCTGC 1 cut(s) 873
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 2 cut(s) 204, 416
CseI GACGC 1 cut(s) 748
CspAI ACCGGT 1 cut(s) 380
CviAII CATG 7 cut(s) 347, 454, 479, 856, 1004, 1088, 1128
DdeI CTNAG 3 cut(s) 353, 437, 889
DpnI GATC 7 cut(s) 118, 233, 639, 687, 945, 961, 1011
DpnII GATC 7 cut(s) 116, 231, 637, 685, 943, 959, 1009
EaeI YGGCCR 1 cut(s) 858
Eam1104I CTCTTC 2 cut(s) 787, 988
EarI CTCTTC 2 cut(s) 787, 988
Eco130I CCWWGG 2 cut(s) 346, 478
Eco24I GRGCYC 1 cut(s) 1025
Eco47I GGWCC 1 cut(s) 204
Eco88I CYCGRG 2 cut(s) 285, 746
EcoO109I RGGNCCY 1 cut(s) 416
EcoT14I CCWWGG 2 cut(s) 346, 478
EcoT38I GRGCYC 1 cut(s) 1025
ErhI CCWWGG 2 cut(s) 346, 478
Esp3I CGTCTC 1 cut(s) 731
FaeI CATG 7 cut(s) 350, 457, 482, 859, 1007, 1091, 1131
FatI CATG 7 cut(s) 346, 453, 478, 855, 1003, 1087, 1127
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 7 cut(s) 72, 472, 475, 663, 711, 743, 795
FokI GGATG 2 cut(s) 986, 1027
FriOI GRGCYC 1 cut(s) 1025
Fsp4HI GCNGC 7 cut(s) 72, 472, 475, 663, 711, 743, 795
FspBI CTAG 3 cut(s) 425, 914, 954
GluI GCNGC 7 cut(s) 72, 472, 475, 663, 711, 743, 795
GsaI CCCAGC 1 cut(s) 973
GsuI CTGGAG 1 cut(s) 839
HaeIII GGCC 3 cut(s) 418, 860, 876
HapII CCGG 2 cut(s) 381, 666
HgaI GACGC 1 cut(s) 748
Hin1II CATG 7 cut(s) 350, 457, 482, 859, 1007, 1091, 1131
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 4 cut(s) 31, 316, 336, 557
HpaII CCGG 2 cut(s) 381, 666
Hpy166II GTNNAC 2 cut(s) 181, 951
Hpy188III TCNNGA 5 cut(s) 44, 86, 311, 730, 818
Hpy8I GTNNAC 2 cut(s) 181, 951
HpyAV CCTTC 7 cut(s) 535, 572, 692, 762, 866, 915, 988
HpyCH4III ACNGT 2 cut(s) 680, 1104
HpyCH4V TGCA 7 cut(s) 8, 265, 333, 474, 486, 626, 1099
HpyF10VI GCNNNNNNNGC 4 cut(s) 160, 290, 483, 748
HpyF3I CTNAG 3 cut(s) 353, 437, 889
Hsp92II CATG 7 cut(s) 350, 457, 482, 859, 1007, 1091, 1131
Kzo9I GATC 7 cut(s) 116, 231, 637, 685, 943, 959, 1009
LmnI GCTCC 1 cut(s) 1020
Lsp1109I GCAGC 7 cut(s) 58, 458, 486, 649, 697, 729, 806
LweI GCATC 2 cut(s) 271, 320
MaeI CTAG 3 cut(s) 425, 914, 954
MaeIII GTNAC 2 cut(s) 28, 824
MalI GATC 7 cut(s) 118, 233, 639, 687, 945, 961, 1011
MboI GATC 7 cut(s) 116, 231, 637, 685, 943, 959, 1009
MboII GAAGA 7 cut(s) 515, 702, 760, 774, 975, 1073, 1076
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 1 cut(s) 231
MhlI GDGCHC 2 cut(s) 590, 1025
MlsI TGGCCA 1 cut(s) 860
MluCI AATT 4 cut(s) 9, 340, 399, 758
MluNI TGGCCA 1 cut(s) 860
MlyI GAGTC 2 cut(s) 25, 345
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 7 cut(s) 124, 318, 367, 490, 595, 600, 756
Mox20I TGGCCA 1 cut(s) 860
MscI TGGCCA 1 cut(s) 860
MseI TTAA 2 cut(s) 102, 404
MslI CAYNNNNRTG 1 cut(s) 1090
Msp20I TGGCCA 1 cut(s) 860
MspI CCGG 2 cut(s) 381, 666
MunI CAATTG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 461
MwoI GCNNNNNNNGC 4 cut(s) 160, 290, 483, 748
NcoI CCATGG 2 cut(s) 346, 478
NdeII GATC 7 cut(s) 116, 231, 637, 685, 943, 959, 1009
NlaIII CATG 7 cut(s) 350, 457, 482, 859, 1007, 1091, 1131
NlaIV GGNNCC 1 cut(s) 1022
NmuCI GTSAC 2 cut(s) 28, 824
NspI RCATGY 1 cut(s) 457
PaeR7I CTCGAG 2 cut(s) 285, 746
PciI ACATGT 1 cut(s) 453
PctI GAATGC 1 cut(s) 461
PfeI GAWTC 2 cut(s) 316, 557
PinAI ACCGGT 1 cut(s) 380
PkrI GCNGC 7 cut(s) 73, 473, 476, 664, 712, 744, 796
PleI GAGTC 2 cut(s) 25, 344
PpsI GAGTC 2 cut(s) 25, 344
PscI ACATGT 1 cut(s) 453
PspFI CCCAGC 1 cut(s) 969
PspN4I GGNNCC 1 cut(s) 1022
PspPI GGNCC 2 cut(s) 204, 416
PspXI VCTCGAGB 2 cut(s) 285, 746
PstI CTGCAG 1 cut(s) 476
PsuI RGATCY 1 cut(s) 231
RseI CAYNNNNRTG 1 cut(s) 1090
SaqAI TTAA 2 cut(s) 102, 404
SatI GCNGC 7 cut(s) 72, 472, 475, 663, 711, 743, 795
Sau3AI GATC 7 cut(s) 116, 231, 637, 685, 943, 959, 1009
Sau96I GGNCC 2 cut(s) 204, 416
SchI GAGTC 2 cut(s) 25, 345
SduI GDGCHC 2 cut(s) 590, 1025
SfaNI GCATC 2 cut(s) 271, 320
SfcI CTRYAG 1 cut(s) 472
Sfr274I CTCGAG 2 cut(s) 285, 746
SinI GGWCC 1 cut(s) 204
SlaI CTCGAG 2 cut(s) 285, 746
SmiMI CAYNNNNRTG 1 cut(s) 1090
SmlI CTYRAG 3 cut(s) 285, 311, 746
SmoI CTYRAG 3 cut(s) 285, 311, 746
SpeI ACTAGT 1 cut(s) 953
Sse9I AATT 4 cut(s) 9, 340, 399, 758
SspMI CTAG 3 cut(s) 425, 914, 954
StyI CCWWGG 2 cut(s) 346, 478
TaaI ACNGT 2 cut(s) 680, 1104
TaqI TCGA 5 cut(s) 96, 286, 385, 688, 747
TasI AATT 4 cut(s) 9, 340, 399, 758
TfiI GAWTC 2 cut(s) 316, 557
Tru1I TTAA 2 cut(s) 102, 404
Tru9I TTAA 2 cut(s) 102, 404
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 2 cut(s) 28, 824
TseI GCWGC 7 cut(s) 71, 471, 474, 662, 710, 742, 794
Tsp45I GTSAC 2 cut(s) 28, 824
TspDTI ATGAA 6 cut(s) 100, 215, 422, 459, 581, 1104
TspRI CASTG 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 204
XapI RAATTY 1 cut(s) 399
XceI RCATGY 1 cut(s) 457
XcmI CCANNNNNNNNNTGG 2 cut(s) 198, 496
XhoI CTCGAG 2 cut(s) 285, 746
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 3 cut(s) 425, 914, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.