Rroxscaffold_3G00270220

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002611
Physical Location & Seq
Reverse (-)
62832098 .. 62835862
3765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00270220.1

Sequence Viewer

Length: 1560 bp
ATGTGCAAGGTGGAGAAGGAGAGAAGAGGAGTGATGGGGATAAAGGGGCTGGGAGGAGGTGGAGATAAGTTGAAGAACTCGCTGCTGCAAACGACGTCGTCTTCGTCTTCGAGGTCGAGAATGAAGCTGTGGATGATAAGGGCGACGACGTCGGTTTTGCTGTGGACGTGCGTCGTGCAATTGACGGCGTTGGGGGATATGTGGGGGCCTAGGGTTTTGAAGGGATGGCCGTCCTGCTTCTCTCAGGAGAGTGCCGTGGCCTCGTCGGTTTTGCAGGACCGGATGATTTCCGTTCCGGCCAGAGTTCTTCCGCCAAAGAGGGTTTACAAAAACAATGGCTACCTGATGGTTTCGTGTAACGGGGGACTCAATCAAATGAGAGCAGCGATATGCGACATGGTTGCTATTGCAAGGTATTTGAATGTCACGCTCGTAGTTCCCGAACTTGATAAAACGTCCTTTTGGGCTGATCCCAGTGAGTTTCAAGACATATTTGATGTGGATCATTTCATCACATCCTTGAGAGATGAGGTTCGGATATTGAAAGAGTTGCCTCCCAGGGTTAAGAGAAGAGTGGAACTAGGAATGGTTTATACCATGCCACCAGTTAGTTGGTCTGACATATCTTATTATCATAATCAGATTCTTCCTCTGATACAAAAATACAAAGTTGTACATTTGAATAAAACTGATGCTAGACTGGCCAATAATGGACAACCCTTGGAGATTCAGAAACTTCGATGCCGAGTAAATTTTAGTGCTCTGCGATTTACTTCTCAAATAGAGGAATTGGGTAGAAGGGTCATAAATCTTCTTAGGGCAAATGGTCCTTTCCTAGTTCTTCATCTTAGATATGAAATGGACATGTTGGCATTTTCTGGCTGTACCCAAGGTTGCAACGCTGAGGAGGTGGAAGAGTTGACAAGGATGAGATATGCTTATCCCTGGTGGAAGGAGAAAATAATAAACTCTGACCTGAAAAGGAAAGATGGTTTGTGTCCTTTGACACCAGAAGAAACTGCTCTCACACTGAGTGCACTTGAAATTGATCCCAACATCCAGATCTATATTGCCGCTGGTGAAATCTACGGTGGTGAGAGGAGAATGGCAAGTCTTGCAAAGGCTTTTCCAAAATTGGTCAGAAAGGAGACACTGTTAGAACCGTCAGACCTCAGGTTCTTTCAAAACCACTCCTCCCAAATGGCGGCATTGGATTATCTCGTCTCGTTGGAGAGTGATATCTTTGTTCCCACATATGATGGAAACATGGCTAAAGTTGTTGAAGGCCATCGCAGATTTCTCGGGTTCAAGAAGACAATTCTACTGGACAGAAGGCTTCTTGTTGATTTGATAGACCAGTACAATGGTGGAGAATTGAATTGGGATGAATTCTCTTCTGCAGTGAAGGAAGCTCATGCAGAACGCATGGGTAACCCAACTAAAAGGTTGATGATCCAAGACAGACCTAAAGAGGAGGACTATTTCTATGCCAACCCAGAAGAGTGTTTGCAATTGTCAGATGATGAGCAATTATCAGATGGGCTATTGAGTAGTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

519

Amino Acids

59.14

Weight (kDa)

8.2

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 114 - 433 4.3e-106 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 98, 152
AciI CCGC 3 cut(s) 311, 1074, 1205
AclWI GGATC 4 cut(s) 464, 510, 1043, 1447
AcoI YGGCCR 3 cut(s) 227, 297, 702
AcsI RAATTY 2 cut(s) 751, 1388
AcyI GRCGYC 2 cut(s) 95, 149
AdeI CACNNNGTG 1 cut(s) 1034
AfaI GTAC 3 cut(s) 675, 886, 1361
AfiI CCNNNNNNNGG 1 cut(s) 1204
AflIII ACRYGT 1 cut(s) 864
AjiI CACGTC 1 cut(s) 168
AjnI CCWGG 2 cut(s) 557, 944
AluBI AGCT 2 cut(s) 127, 1412
AluI AGCT 2 cut(s) 127, 1412
Alw21I GWGCWC 2 cut(s) 763, 1039
Alw26I GTCTC 2 cut(s) 1142, 1228
Alw44I GTGCAC 1 cut(s) 1035
AlwI GGATC 4 cut(s) 464, 510, 1043, 1447
Ama87I CYCGRG 1 cut(s) 1301
AoxI GGCC 6 cut(s) 206, 227, 258, 297, 702, 1285
ApaLI GTGCAC 1 cut(s) 1035
ApeKI GCWGC 3 cut(s) 82, 85, 383
ApoI RAATTY 2 cut(s) 751, 1388
ArsI GACNNNNNNTTYG 4 cut(s) 85, 117, 139, 171
AspA2I CCTAGG 1 cut(s) 209
AspS9I GGNCC 3 cut(s) 206, 277, 827
AsuHPI GGTGA 2 cut(s) 1091, 1106
AvaI CYCGRG 1 cut(s) 1301
AvaII GGWCC 2 cut(s) 277, 827
AvrII CCTAGG 1 cut(s) 209
AxyI CCTNAGG 1 cut(s) 1172
BaeGI GKGCMC 1 cut(s) 1039
BalI TGGCCA 1 cut(s) 704
BbsI GAAGAC 3 cut(s) 93, 99, 1319
Bbv12I GWGCWC 2 cut(s) 763, 1039
BbvCI CCTCAGC 1 cut(s) 903
BbvI GCAGC 3 cut(s) 69, 72, 395
BccI CCATC 7 cut(s) 28, 219, 340, 983, 1253, 1296, 1532
BceAI ACGGC 3 cut(s) 201, 214, 239
BcgI CGANNNNNNTGC 4 cut(s) 383, 417, 1282, 1316
BciT130I CCWGG 2 cut(s) 559, 946
BcoDI GTCTC 2 cut(s) 1142, 1228
BfaI CTAG 4 cut(s) 210, 581, 696, 836
BfmI CTRYAG 1 cut(s) 1398
BglII AGATCT 1 cut(s) 1062
BisI GCNGC 5 cut(s) 83, 86, 384, 1074, 1206
BlnI CCTAGG 1 cut(s) 209
BlsI GCNGC 5 cut(s) 84, 87, 385, 1075, 1207
Bme1390I CCNGG 2 cut(s) 559, 946
Bme18I GGWCC 2 cut(s) 277, 827
BmeT110I CYCGRG 1 cut(s) 1301
BmgBI CACGTC 1 cut(s) 168
BmgT120I GGNCC 3 cut(s) 206, 277, 827
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 2 cut(s) 559, 946
BmrI ACTGGG 1 cut(s) 468
BmsI GCATC 2 cut(s) 682, 731
BmuI ACTGGG 1 cut(s) 468
BoxI GACNNNNGTC 1 cut(s) 170
BpiI GAAGAC 3 cut(s) 93, 99, 1319
Bpu10I CCTNAGC 1 cut(s) 903
BpuEI CTTGAG 1 cut(s) 541
BsaBI GATNNNNATC 1 cut(s) 501
BsaHI GRCGYC 2 cut(s) 95, 149
BsaJI CCNNGG 7 cut(s) 209, 255, 557, 558, 720, 889, 944
BsaWI WCCGGW 1 cut(s) 279
BsaXI ACNNNNNCTCC 4 cut(s) 1139, 1169, 1178, 1208
Bsc4I CCNNNNNNNGG 1 cut(s) 1204
Bse1I ACTGG 5 cut(s) 474, 605, 705, 1329, 1357
Bse21I CCTNAGG 1 cut(s) 1172
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 2 cut(s) 559, 946
BseDI CCNNGG 7 cut(s) 209, 255, 557, 558, 720, 889, 944
BseGI GGATG 7 cut(s) 138, 230, 288, 515, 933, 1056, 1390
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 1 cut(s) 1204
BseMII CTCAG 4 cut(s) 257, 894, 1022, 1186
BseNI ACTGG 5 cut(s) 474, 605, 705, 1329, 1357
BseRI GAGGAG 6 cut(s) 42, 69, 920, 1114, 1183, 1487
BseSI GKGCMC 1 cut(s) 1039
BseXI GCAGC 3 cut(s) 69, 72, 395
BseYI CCCAGC 1 cut(s) 49
BshFI GGCC 6 cut(s) 208, 229, 260, 299, 704, 1287
BsiHKAI GWGCWC 2 cut(s) 763, 1039
BsiHKCI CYCGRG 1 cut(s) 1301
BsiSI CCGG 2 cut(s) 280, 296
BslFI GGGAC 1 cut(s) 378
BslI CCNNNNNNNGG 1 cut(s) 1204
BsmAI GTCTC 2 cut(s) 1142, 1228
BsmBI CGTCTC 1 cut(s) 1228
BsmFI GGGAC 1 cut(s) 378
BsnI GGCC 6 cut(s) 208, 229, 260, 299, 704, 1287
BsoBI CYCGRG 1 cut(s) 1301
Bsp1286I GDGCHC 2 cut(s) 763, 1039
Bsp1407I TGTACA 1 cut(s) 673
Bsp143I GATC 5 cut(s) 469, 502, 1048, 1062, 1452
BspACI CCGC 3 cut(s) 311, 1074, 1205
BspANI GGCC 6 cut(s) 208, 229, 260, 299, 704, 1287
BspCNI CTCAG 4 cut(s) 256, 895, 1023, 1185
BspLI GGNNCC 1 cut(s) 207
BspMAI CTGCAG 1 cut(s) 1402
BspPI GGATC 4 cut(s) 464, 510, 1043, 1447
BsrGI TGTACA 1 cut(s) 673
BsrI ACTGG 5 cut(s) 474, 605, 705, 1329, 1357
BssECI CCNNGG 7 cut(s) 209, 255, 557, 558, 720, 889, 944
BssMI GATC 5 cut(s) 469, 502, 1048, 1062, 1452
BssNI GRCGYC 2 cut(s) 95, 149
BssT1I CCWWGG 3 cut(s) 209, 720, 889
Bst2UI CCWGG 2 cut(s) 559, 946
Bst4CI ACNGT 3 cut(s) 1091, 1155, 1164
Bst6I CTCTTC 5 cut(s) 19, 565, 909, 1399, 1494
BstACI GRCGYC 2 cut(s) 95, 149
BstAPI GCANNNNNTGC 1 cut(s) 1115
BstAUI TGTACA 1 cut(s) 673
BstDEI CTNAG 6 cut(s) 243, 815, 848, 903, 1031, 1172
BstDSI CCRYGG 1 cut(s) 255
BstEII GGTNACC 1 cut(s) 1430
BstF5I GGATG 7 cut(s) 138, 230, 288, 515, 933, 1056, 1390
BstKTI GATC 5 cut(s) 472, 505, 1051, 1065, 1455
BstMAI GTCTC 2 cut(s) 1142, 1228
BstMBI GATC 5 cut(s) 469, 502, 1048, 1062, 1452
BstMWI GCNNNNNNNGC 2 cut(s) 701, 1115
BstNI CCWGG 2 cut(s) 559, 946
BstNSI RCATGY 1 cut(s) 868
BstPAI GACNNNNGTC 1 cut(s) 170
BstPI GGTNACC 1 cut(s) 1430
BstSCI CCNGG 2 cut(s) 557, 944
BstSFI CTRYAG 1 cut(s) 1398
BstSLI GKGCMC 1 cut(s) 1039
BstV1I GCAGC 3 cut(s) 69, 72, 395
BstV2I GAAGAC 3 cut(s) 93, 99, 1319
BstX2I RGATCY 1 cut(s) 1062
BstXI CCANNNNNNTGG 2 cut(s) 612, 1364
BstYI RGATCY 1 cut(s) 1062
Bsu36I CCTNAGG 1 cut(s) 1172
BsuRI GGCC 6 cut(s) 208, 229, 260, 299, 704, 1287
BtgI CCRYGG 1 cut(s) 255
BtgZI GCGATG 1 cut(s) 1274
BtrI CACGTC 1 cut(s) 168
BtsCI GGATG 7 cut(s) 138, 230, 288, 515, 933, 1056, 1390
BtsI GCAGTG 1 cut(s) 1407
BtsIMutI CAGTG 4 cut(s) 481, 1028, 1151, 1407
Cfr13I GGNCC 3 cut(s) 206, 277, 827
CseI GACGC 1 cut(s) 160
Csp6I GTAC 3 cut(s) 674, 885, 1360
CviAII CATG 6 cut(s) 397, 598, 865, 1267, 1415, 1426
CviQI GTAC 3 cut(s) 674, 885, 1360
DdeI CTNAG 6 cut(s) 243, 815, 848, 903, 1031, 1172
DpnI GATC 5 cut(s) 471, 504, 1050, 1064, 1454
DpnII GATC 5 cut(s) 469, 502, 1048, 1062, 1452
DraIII CACNNNGTG 1 cut(s) 1034
EaeI YGGCCR 3 cut(s) 227, 297, 702
Eam1104I CTCTTC 5 cut(s) 19, 565, 909, 1399, 1494
EarI CTCTTC 5 cut(s) 19, 565, 909, 1399, 1494
EciI GGCGGA 1 cut(s) 300
Eco130I CCWWGG 3 cut(s) 209, 720, 889
Eco32I GATATC 1 cut(s) 1240
Eco47I GGWCC 2 cut(s) 277, 827
Eco81I CCTNAGG 1 cut(s) 1172
Eco88I CYCGRG 1 cut(s) 1301
Eco91I GGTNACC 1 cut(s) 1430
EcoO109I RGGNCCY 1 cut(s) 206
EcoO65I GGTNACC 1 cut(s) 1430
EcoRI GAATTC 1 cut(s) 1388
EcoRII CCWGG 2 cut(s) 557, 944
EcoRV GATATC 1 cut(s) 1240
EcoT14I CCWWGG 3 cut(s) 209, 720, 889
ErhI CCWWGG 3 cut(s) 209, 720, 889
Esp3I CGTCTC 1 cut(s) 1228
FaeI CATG 6 cut(s) 400, 601, 868, 1270, 1418, 1429
FaqI GGGAC 1 cut(s) 378
FatI CATG 6 cut(s) 396, 597, 864, 1266, 1414, 1425
FauNDI CATATG 1 cut(s) 1255
Fnu4HI GCNGC 5 cut(s) 83, 86, 384, 1074, 1206
FokI GGATG 7 cut(s) 145, 237, 295, 502, 940, 1043, 1397
Fsp4HI GCNGC 5 cut(s) 83, 86, 384, 1074, 1206
FspBI CTAG 4 cut(s) 210, 581, 696, 836
GluI GCNGC 5 cut(s) 83, 86, 384, 1074, 1206
GsaI CCCAGC 1 cut(s) 53
HaeIII GGCC 6 cut(s) 208, 229, 260, 299, 704, 1287
HapII CCGG 2 cut(s) 280, 296
HgaI GACGC 1 cut(s) 160
Hin1I GRCGYC 2 cut(s) 95, 149
Hin1II CATG 6 cut(s) 400, 601, 868, 1270, 1418, 1429
HincII GTYRAC 1 cut(s) 921
HindII GTYRAC 1 cut(s) 921
HinfI GANTC 3 cut(s) 366, 643, 727
HpaII CCGG 2 cut(s) 280, 296
HphI GGTGA 2 cut(s) 1091, 1106
Hpy166II GTNNAC 4 cut(s) 165, 325, 921, 1037
Hpy188III TCNNGA 6 cut(s) 117, 245, 440, 485, 1060, 1309
Hpy8I GTNNAC 4 cut(s) 165, 325, 921, 1037
Hpy99I CGWCG 7 cut(s) 97, 100, 148, 151, 154, 176, 268
HpyAV CCTTC 7 cut(s) 10, 214, 792, 946, 1277, 1326, 1399
HpyCH4III ACNGT 3 cut(s) 1091, 1155, 1164
HpyCH4IV ACGT 4 cut(s) 95, 149, 167, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 701, 1115
HpyF3I CTNAG 6 cut(s) 243, 815, 848, 903, 1031, 1172
HpySE526I ACGT 4 cut(s) 95, 149, 167, 455
Hsp92I GRCGYC 2 cut(s) 95, 149
Hsp92II CATG 6 cut(s) 400, 601, 868, 1270, 1418, 1429
Kzo9I GATC 5 cut(s) 469, 502, 1048, 1062, 1452
Lsp1109I GCAGC 3 cut(s) 69, 72, 395
LweI GCATC 2 cut(s) 682, 731
MaeI CTAG 4 cut(s) 210, 581, 696, 836
MaeII ACGT 4 cut(s) 95, 149, 167, 455
MaeIII GTNAC 3 cut(s) 356, 424, 1430
MalI GATC 5 cut(s) 471, 504, 1050, 1064, 1454
MboI GATC 5 cut(s) 469, 502, 1048, 1062, 1452
MfeI CAATTG 2 cut(s) 179, 1511
MflI RGATCY 1 cut(s) 1062
MhlI GDGCHC 2 cut(s) 763, 1039
MlsI TGGCCA 1 cut(s) 704
MluNI TGGCCA 1 cut(s) 704
MlyI GAGTC 1 cut(s) 360
MmeI TCCRAC 1 cut(s) 1209
Mox20I TGGCCA 1 cut(s) 704
MscI TGGCCA 1 cut(s) 704
MseI TTAA 1 cut(s) 564
Msp20I TGGCCA 1 cut(s) 704
MspA1I CMGCKG 1 cut(s) 1076
MspI CCGG 2 cut(s) 280, 296
MspR9I CCNGG 2 cut(s) 559, 946
MunI CAATTG 2 cut(s) 179, 1511
MvaI CCWGG 2 cut(s) 559, 946
MwoI GCNNNNNNNGC 2 cut(s) 701, 1115
NdeI CATATG 1 cut(s) 1255
NdeII GATC 5 cut(s) 469, 502, 1048, 1062, 1452
NlaIII CATG 6 cut(s) 400, 601, 868, 1270, 1418, 1429
NlaIV GGNNCC 1 cut(s) 207
NmeAIII GCCGAG 1 cut(s) 770
NmuCI GTSAC 1 cut(s) 424
NspI RCATGY 1 cut(s) 868
PasI CCCWGGG 1 cut(s) 558
PciI ACATGT 1 cut(s) 864
PcsI WCGNNNNNNNCGW 2 cut(s) 101, 438
PfeI GAWTC 2 cut(s) 643, 727
PflFI GACNNNGTC 2 cut(s) 97, 148
PkrI GCNGC 5 cut(s) 84, 87, 385, 1075, 1207
PleI GAGTC 1 cut(s) 360
PpsI GAGTC 1 cut(s) 360
PscI ACATGT 1 cut(s) 864
PshAI GACNNNNGTC 1 cut(s) 170
Psp6I CCWGG 2 cut(s) 557, 944
PspEI GGTNACC 1 cut(s) 1430
PspFI CCCAGC 1 cut(s) 49
PspGI CCWGG 2 cut(s) 557, 944
PspN4I GGNNCC 1 cut(s) 207
PspPI GGNCC 3 cut(s) 206, 277, 827
PstI CTGCAG 1 cut(s) 1402
PsuI RGATCY 1 cut(s) 1062
PsyI GACNNNGTC 2 cut(s) 97, 148
RsaI GTAC 3 cut(s) 675, 886, 1361
RsaNI GTAC 3 cut(s) 674, 885, 1360
SaqAI TTAA 1 cut(s) 564
SatI GCNGC 5 cut(s) 83, 86, 384, 1074, 1206
Sau3AI GATC 5 cut(s) 469, 502, 1048, 1062, 1452
Sau96I GGNCC 3 cut(s) 206, 277, 827
SchI GAGTC 1 cut(s) 360
ScrFI CCNGG 2 cut(s) 559, 946
SduI GDGCHC 2 cut(s) 763, 1039
SfaNI GCATC 2 cut(s) 682, 731
SfcI CTRYAG 1 cut(s) 1398
SinI GGWCC 2 cut(s) 277, 827
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
SsiI CCGC 3 cut(s) 311, 1074, 1205
SspMI CTAG 4 cut(s) 210, 581, 696, 836
StyD4I CCNGG 2 cut(s) 557, 944
StyI CCWWGG 3 cut(s) 209, 720, 889
TaaI ACNGT 3 cut(s) 1091, 1155, 1164
TaiI ACGT 4 cut(s) 98, 152, 170, 458
TaqI TCGA 3 cut(s) 110, 116, 739
TatI WGTACW 2 cut(s) 673, 1359
TauI GCSGC 2 cut(s) 1076, 1208
TfiI GAWTC 2 cut(s) 643, 727
Tru1I TTAA 1 cut(s) 564
Tru9I TTAA 1 cut(s) 564
TscAI CASTG 4 cut(s) 481, 1035, 1158, 1407
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 3 cut(s) 82, 85, 383
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 5 cut(s) 137, 499, 833, 870, 1401
TspGWI ACGGA 1 cut(s) 280
TspRI CASTG 4 cut(s) 481, 1035, 1158, 1407
Tth111I GACNNNGTC 2 cut(s) 97, 148
VneI GTGCAC 1 cut(s) 1035
VpaK11BI GGWCC 2 cut(s) 277, 827
XapI RAATTY 2 cut(s) 751, 1388
XceI RCATGY 1 cut(s) 868
XcmI CCANNNNNNNNNTGG 2 cut(s) 609, 1364
XmaJI CCTAGG 1 cut(s) 209
XspI CTAG 4 cut(s) 210, 581, 696, 836
ZraI GACGTC 2 cut(s) 96, 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.