MD17G1066500.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002611
Physical Location & Seq
Forward (+)
5373008 .. 5375600
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1066500.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGTGCAAAGGTGAGGACTTTGAGAAGTGTGAGTACAGAGAAACAATGAAGGTGGGATTTAAGTTGATGGGGAGAGTTGAAAGGTTGAAAAGCTCAGTGTTGAAGGTGAACTCATGGCTGCTGATCATCCGGGCGATCGTGACGGCGTTGGTGTGGGCGTCGGCGGTGCAGCTGGTTGGGATTTGGTTGATTAGGGGGCCTAGTTTGTTCAATGTTTGGCCTCCTTGCACTCACTTGGAATTGCCTGCTCATGCTGTAGAATTGACTAATAAGCCTCCAATGCCAATTGCTCTCCCTCCAAAAAGGGTTTACAAAAATAATGGCTATCTCATGGTTTCTTGCAATGGAGGACTGAATCAAATGCGAGCGGCAATCTGTGATATGGTTGCAGTTGCCAGATACTTGAATGTCACCCTTATAGTTCCTGAGCTGGATAAAAACTCCTTTTGGGCTGCGAGTGAGTTTCAGGACGTTTTCGATGTTGATCATTTCATTGCATCGTTGAGAGACGAGGTTCGGATATTGAGGATATTGCCACCAAAGCTGAAGAAAAGACCTGCATACTCGTTGCCACCAGTTAGTTGGTCTAATATGTCCTACTACCTTCATCAGATCCTGCCTCTGGTGCAGAAGTATAAAGTAGTACATTTGAATAAAACCGATGCTCGAGTAGCTAACAATGGGCTACCTCTGGAGTTCCAGAAGCTGCGCTGCAGAGTTAATTTCCATGCTCTGAGATTCACTTCCCAGATAGAGGAAGTCGGTAGAAAGCTTGTCGGAATTTTGAGAGAAAAAGGCCCTTTTCTGGCTCTTCATCTCCGATACGAAATGGATATGTTGGCATTCTCTGGCTGCAGTCATGGGTGCAGCAATGATGAGGTAGAAGAACTCACAAGAATGAGATATGCTAATCCCTTGTGGAAGATAAAAGACATAAATTCTGAAACAAAGAGGCTAGAAGGTTTGTGCCCACTAACACCAGAGGAAACTGCTCTAGTATTGGTTGCACTCGGTGTTGATCACAATGTCCAGATTTATATTGCCGCTGGAGAAATGTATGGTGGAAACAGAAGGATGGAAGGTTTGAAAGCTGCTTTTCCGAATTTGGTCAGAAAAGAGACGCTGCTGGAGCCTTCTCTCGGTGATTTCCCGAGGTTCTACCCGAACAAACTCTTCCAAAATGGGCAGATGATTGGACTATCTAGTCTCCCTAGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

407

Amino Acids

46.21

Weight (kDa)

9.22

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 109 - 378 8e-87 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1203
Acc36I ACCTGC 1 cut(s) 565
AccBSI CCGCTC 1 cut(s) 368
AciI CCGC 3 cut(s) 164, 368, 1044
AclWI GGATC 1 cut(s) 607
AcsI RAATTY 3 cut(s) 780, 937, 1102
AcuI CTGAAG 1 cut(s) 566
AcyI GRCGYC 1 cut(s) 158
AdeI CACNNNGTG 1 cut(s) 1013
AfaI GTAC 2 cut(s) 35, 645
AfiI CCNNNNNNNGG 4 cut(s) 622, 754, 805, 1139
AgsI TTSAA 7 cut(s) 80, 88, 103, 211, 406, 652, 1087
AluBI AGCT 8 cut(s) 93, 172, 430, 544, 674, 706, 772, 1091
AluI AGCT 8 cut(s) 93, 172, 430, 544, 674, 706, 772, 1091
Alw26I GTCTC 3 cut(s) 501, 1112, 1211
AlwI GGATC 1 cut(s) 607
AlwNI CAGNNNCTG 2 cut(s) 616, 706
Ama87I CYCGRG 2 cut(s) 666, 1150
AoxI GGCC 3 cut(s) 197, 218, 796
ApeKI GCWGC 9 cut(s) 118, 169, 452, 706, 711, 852, 867, 1091, 1123
ApoI RAATTY 3 cut(s) 780, 937, 1102
AspLEI GCGC 1 cut(s) 711
AspS9I GGNCC 2 cut(s) 197, 797
AsuC2I CCSGG 1 cut(s) 131
AsuHPI GGTGA 4 cut(s) 23, 118, 403, 1154
AvaI CYCGRG 2 cut(s) 666, 1150
BaeGI GKGCMC 1 cut(s) 971
BarI GAAGNNNNNNTAC 2 cut(s) 17, 49
BbvI GCAGC 9 cut(s) 105, 181, 439, 693, 698, 839, 879, 1078, 1110
BccI CCATC 2 cut(s) 61, 1069
BceAI ACGGC 1 cut(s) 159
BclI TGATCA 3 cut(s) 123, 484, 1018
BcnI CCSGG 1 cut(s) 131
BcoDI GTCTC 3 cut(s) 501, 1112, 1211
BfaI CTAG 5 cut(s) 201, 956, 995, 1203, 1212
BfmI CTRYAG 3 cut(s) 255, 712, 853
BfuAI ACCTGC 1 cut(s) 565
Bme1390I CCNGG 1 cut(s) 131
BmeT110I CYCGRG 2 cut(s) 666, 1150
BmgT120I GGNCC 2 cut(s) 197, 797
BmiI GGNNCC 2 cut(s) 198, 1131
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 2 cut(s) 506, 652
BpmI CTGGAG 3 cut(s) 713, 1068, 1148
Bpu10I CCTNAGC 1 cut(s) 426
BpuMI CCSGG 1 cut(s) 131
BsaBI GATNNNNATC 1 cut(s) 483
BsaHI GRCGYC 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 1151
Bsc4I CCNNNNNNNGG 4 cut(s) 622, 754, 805, 1139
Bse1I ACTGG 1 cut(s) 575
Bse3DI GCAATG 3 cut(s) 349, 492, 877
Bse8I GATNNNNATC 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 1151
BseGI GGATG 2 cut(s) 126, 1080
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 4 cut(s) 622, 754, 805, 1139
BseMI GCAATG 3 cut(s) 349, 492, 877
BseMII CTCAG 3 cut(s) 108, 417, 725
BseNI ACTGG 1 cut(s) 575
BseSI GKGCMC 1 cut(s) 971
BseXI GCAGC 9 cut(s) 105, 181, 439, 693, 698, 839, 879, 1078, 1110
BsgI GTGCAG 3 cut(s) 188, 647, 886
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 3 cut(s) 199, 220, 798
BsiEI CGRYCG 1 cut(s) 138
BsiHKCI CYCGRG 2 cut(s) 666, 1150
BsiSI CCGG 1 cut(s) 130
BslI CCNNNNNNNGG 4 cut(s) 622, 754, 805, 1139
BsmAI GTCTC 3 cut(s) 501, 1112, 1211
BsmBI CGTCTC 2 cut(s) 501, 1112
BsmI GAATGC 1 cut(s) 842
BsnI GGCC 3 cut(s) 199, 220, 798
BsoBI CYCGRG 2 cut(s) 666, 1150
Bsp1286I GDGCHC 1 cut(s) 971
Bsp143I GATC 5 cut(s) 123, 135, 484, 612, 1018
BspACI CCGC 3 cut(s) 164, 368, 1044
BspANI GGCC 3 cut(s) 199, 220, 798
BspCNI CTCAG 3 cut(s) 107, 418, 726
BspLI GGNNCC 2 cut(s) 198, 1131
BspMAI CTGCAG 2 cut(s) 716, 857
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 1 cut(s) 607
BspQI GCTCTTC 1 cut(s) 816
BsrBI CCGCTC 1 cut(s) 368
BsrDI GCAATG 3 cut(s) 349, 492, 877
BsrI ACTGG 1 cut(s) 575
BssECI CCNNGG 1 cut(s) 1151
BssMI GATC 5 cut(s) 123, 135, 484, 612, 1018
BssNI GRCGYC 1 cut(s) 158
Bst6I CTCTTC 2 cut(s) 816, 1178
BstACI GRCGYC 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 246, 366
BstDEI CTNAG 3 cut(s) 94, 426, 734
BstF5I GGATG 2 cut(s) 126, 1080
BstHHI GCGC 1 cut(s) 711
BstKTI GATC 5 cut(s) 126, 138, 487, 615, 1021
BstMAI GTCTC 3 cut(s) 501, 1112, 1211
BstMBI GATC 5 cut(s) 123, 135, 484, 612, 1018
BstMCI CGRYCG 1 cut(s) 138
BstMWI GCNNNNNNNGC 5 cut(s) 280, 541, 625, 671, 1129
BstSCI CCNGG 1 cut(s) 129
BstSFI CTRYAG 3 cut(s) 255, 712, 853
BstSLI GKGCMC 1 cut(s) 971
BstV1I GCAGC 9 cut(s) 105, 181, 439, 693, 698, 839, 879, 1078, 1110
BstX2I RGATCY 1 cut(s) 612
BstXI CCANNNNNNTGG 1 cut(s) 582
BstYI RGATCY 1 cut(s) 612
BsuRI GGCC 3 cut(s) 199, 220, 798
BtsCI GGATG 2 cut(s) 126, 1080
BtsIMutI CAGTG 1 cut(s) 102
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 2 cut(s) 246, 366
CaiI CAGNNNCTG 2 cut(s) 616, 706
CfoI GCGC 1 cut(s) 711
Cfr13I GGNCC 2 cut(s) 197, 797
CseI GACGC 2 cut(s) 147, 1129
Csp6I GTAC 2 cut(s) 34, 644
CspCI CAANNNNNGTGG 2 cut(s) 33, 68
CviAII CATG 5 cut(s) 114, 251, 331, 728, 860
CviQI GTAC 2 cut(s) 34, 644
DdeI CTNAG 3 cut(s) 94, 426, 734
DpnI GATC 5 cut(s) 125, 137, 486, 614, 1020
DpnII GATC 5 cut(s) 123, 135, 484, 612, 1018
DraIII CACNNNGTG 1 cut(s) 1013
DrdI GACNNNNNNGTC 1 cut(s) 1203
DseDI GACNNNNNNGTC 1 cut(s) 1203
Eam1104I CTCTTC 2 cut(s) 816, 1178
EarI CTCTTC 2 cut(s) 816, 1178
Eco57I CTGAAG 1 cut(s) 566
Eco88I CYCGRG 2 cut(s) 666, 1150
EcoO109I RGGNCCY 2 cut(s) 197, 797
Esp3I CGTCTC 2 cut(s) 501, 1112
FaeI CATG 5 cut(s) 117, 254, 334, 731, 863
FatI CATG 5 cut(s) 113, 250, 330, 727, 859
FbaI TGATCA 3 cut(s) 123, 484, 1018
FokI GGATG 2 cut(s) 113, 1087
FspBI CTAG 5 cut(s) 201, 956, 995, 1203, 1212
GlaI GCGC 1 cut(s) 710
GsuI CTGGAG 3 cut(s) 713, 1068, 1148
HaeIII GGCC 3 cut(s) 199, 220, 798
HapII CCGG 1 cut(s) 130
HgaI GACGC 2 cut(s) 147, 1129
HhaI GCGC 1 cut(s) 711
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 5 cut(s) 117, 254, 334, 731, 863
Hin6I GCGC 1 cut(s) 709
HinP1I GCGC 1 cut(s) 709
HindIII AAGCTT 1 cut(s) 770
HinfI GANTC 2 cut(s) 355, 738
HpaII CCGG 1 cut(s) 130
HphI GGTGA 4 cut(s) 23, 118, 403, 1154
Hpy166II GTNNAC 2 cut(s) 109, 310
Hpy188I TCNGA 8 cut(s) 519, 612, 735, 779, 821, 943, 1101, 1112
Hpy188III TCNNGA 7 cut(s) 139, 425, 467, 692, 700, 1030, 1150
Hpy8I GTNNAC 2 cut(s) 109, 310
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 7 cut(s) 43, 97, 614, 953, 1065, 1073, 1143
HpyCH4IV ACGT 1 cut(s) 471
HpyF10VI GCNNNNNNNGC 5 cut(s) 280, 541, 625, 671, 1129
HpyF3I CTNAG 3 cut(s) 94, 426, 734
HpySE526I ACGT 1 cut(s) 471
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 5 cut(s) 117, 254, 334, 731, 863
HspAI GCGC 1 cut(s) 709
Ksp22I TGATCA 3 cut(s) 123, 484, 1018
Kzo9I GATC 5 cut(s) 123, 135, 484, 612, 1018
LguI GCTCTTC 1 cut(s) 816
LmnI GCTCC 1 cut(s) 1129
Lsp1109I GCAGC 9 cut(s) 105, 181, 439, 693, 698, 839, 879, 1078, 1110
LweI GCATC 2 cut(s) 506, 652
MaeI CTAG 5 cut(s) 201, 956, 995, 1203, 1212
MaeII ACGT 1 cut(s) 471
MaeIII GTNAC 2 cut(s) 139, 409
MalI GATC 5 cut(s) 125, 137, 486, 614, 1020
MbiI CCGCTC 1 cut(s) 368
MboI GATC 5 cut(s) 123, 135, 484, 612, 1018
MboII GAAGA 5 cut(s) 559, 803, 896, 934, 1165
MfeI CAATTG 1 cut(s) 285
MflI RGATCY 1 cut(s) 612
MhlI GDGCHC 1 cut(s) 971
MluCI AATT 7 cut(s) 239, 260, 285, 721, 780, 937, 1102
MmeI TCCRAC 1 cut(s) 757
MseI TTAA 2 cut(s) 60, 720
MslI CAYNNNNRTG 1 cut(s) 896
MspA1I CMGCKG 2 cut(s) 172, 1046
MspI CCGG 1 cut(s) 130
MspR9I CCNGG 1 cut(s) 131
MunI CAATTG 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 842
MwoI GCNNNNNNNGC 5 cut(s) 280, 541, 625, 671, 1129
NciI CCSGG 1 cut(s) 131
NdeII GATC 5 cut(s) 123, 135, 484, 612, 1018
NlaIII CATG 5 cut(s) 117, 254, 334, 731, 863
NlaIV GGNNCC 2 cut(s) 198, 1131
NmuCI GTSAC 2 cut(s) 139, 409
PaeR7I CTCGAG 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 816
PctI GAATGC 1 cut(s) 842
PfeI GAWTC 2 cut(s) 355, 738
Ple19I CGATCG 1 cut(s) 138
PspN4I GGNNCC 2 cut(s) 198, 1131
PspPI GGNCC 2 cut(s) 197, 797
PspXI VCTCGAGB 1 cut(s) 666
PstI CTGCAG 2 cut(s) 716, 857
PstNI CAGNNNCTG 2 cut(s) 616, 706
PsuI RGATCY 1 cut(s) 612
PvuI CGATCG 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 172
RsaI GTAC 2 cut(s) 35, 645
RsaNI GTAC 2 cut(s) 34, 644
RseI CAYNNNNRTG 1 cut(s) 896
SapI GCTCTTC 1 cut(s) 816
SaqAI TTAA 2 cut(s) 60, 720
Sau3AI GATC 5 cut(s) 123, 135, 484, 612, 1018
Sau96I GGNCC 2 cut(s) 197, 797
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 1 cut(s) 971
SfaNI GCATC 2 cut(s) 506, 652
SfcI CTRYAG 3 cut(s) 255, 712, 853
Sfr274I CTCGAG 1 cut(s) 666
SlaI CTCGAG 1 cut(s) 666
SmiMI CAYNNNNRTG 1 cut(s) 896
SmlI CTYRAG 1 cut(s) 666
SmoI CTYRAG 1 cut(s) 666
Sse9I AATT 7 cut(s) 239, 260, 285, 721, 780, 937, 1102
SsiI CCGC 3 cut(s) 164, 368, 1044
SspMI CTAG 5 cut(s) 201, 956, 995, 1203, 1212
StyD4I CCNGG 1 cut(s) 129
TaiI ACGT 1 cut(s) 474
TaqI TCGA 2 cut(s) 477, 667
TasI AATT 7 cut(s) 239, 260, 285, 721, 780, 937, 1102
TatI WGTACW 2 cut(s) 33, 643
TauI GCSGC 2 cut(s) 371, 1046
TfiI GAWTC 2 cut(s) 355, 738
Tru1I TTAA 2 cut(s) 60, 720
Tru9I TTAA 2 cut(s) 60, 720
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 2 cut(s) 139, 409
TseI GCWGC 9 cut(s) 118, 169, 452, 706, 711, 852, 867, 1091, 1123
Tsp45I GTSAC 2 cut(s) 139, 409
TspDTI ATGAA 4 cut(s) 62, 481, 596, 803
TspRI CASTG 1 cut(s) 102
XapI RAATTY 3 cut(s) 780, 937, 1102
XcmI CCANNNNNNNNNTGG 1 cut(s) 579
XhoI CTCGAG 1 cut(s) 666
XspI CTAG 5 cut(s) 201, 956, 995, 1203, 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.