MD09G1075800.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002611
Physical Location & Seq
Forward (+)
5351109 .. 5354743
3635 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1075800.v1.1.491

Sequence Viewer

Length: 1515 bp
ATGTGCAAAGCTGAGGCCTTTGAGAAGTTTAATTACGGAGAAGCAATGAAGGTGGGATTTAAGTTGATAAGGAGAGTTGAAAGGTTGAAGAGCTCAGTGTTGAAGAACTCATGGCTGCTGATCATCCGGGCGATCGTTACAGCGTTGGTGTGGGCGTCGGCGGTGCAGCTGGTTGGGATTTGGCTGATTAGGGGGCCTAGTTTGTTCAATGTTTGGCGTCCTTGCACTCAGTTGGAGTTGCCTGTTCATGCCGTTGAATTGGCTAACAAGCTTCCAATGCCAATTGTTCTCCCACCAAAGAGGGTCTACAAAAACAATGGCTATCTCATGGTTTCTTGCAATGGAGGATTGAATCAAATGCGAGCGGCAATCTGTGATATGGTTGCAGTTGCCAGATACTTGAATGTCACACTTATAGTTCCCGAGCTAGATAAAAACTCCTTTTGGGCTGCAAGTGAGTTTCAGGACATTTTTGATGTTGATCATTTCATTACATCTTTGAGAGATGAGGTTCGAATATTAAGGAAATTGCCACCGAAGCTCAGGAAAAGAACTGTATACTCAATGCCACCGGTTAGTTGGTCTAACATGTCCTACTACCTTCATCAGATCCTGCCTCTGGTGCAGAAGTATAAAGTAGTACATTTGAATAAAACTGATGCTCGAGTAGCTAACAATGGGCTACCTCTTGAGTTCCAGAAGCTGCGCTGCCGGGTTAATTTCCATGCTCTGAGATTCACTTCCCAGATTGAGGAAATCGGTAGAAAGCTTCTCAGGATTTTGAGAGAAAAGGGCCCTTTTCTGGCTCTTCATCTCCGATACGAAATGGATATGTTGGCATTCTCTGGCTGCAGTCATGGTTGCAGCAATGATGAGGAAGAAGAACTCACCAAAATGCGATATGCTAACCCCTTGTGGAAGATCAAAGACATAAATTCGGAAACGAAAAGGCTAGAAGGCTTGTGCCCCCTAACACCAGAGGAAACTGCTCTGGTGTTGGTTGCACTTGGAATTGATCACAACGTCCAGATTTATATCGCCTCTGGAGAAATGTATGGCGGAAACAGAAGGATGCAAGGTTTAAAAGCTGCTTTTCCGAATTTGGTCAGAAAAGAGACGCTGCTGGAGCCTTCGGATTTGAGGTTTTTCCTGAACCACTCTTCCCAAATGGCAGCGTTGGACTATCTAGTCTCCTTAGAGAGTGATATATTTATTCCTACGTACGATGGAAACATGGCTAAAGTTGTTGAAGGCCAACGCAGATTCTTGGGTTTCAAAAAGACAGTTTTATTAGACAGAAGGCTACTGATTGGATTAATAGAACAATTCAGCACTGGCTCATTGAGCTGGGATGAGTTCTCTTACATTGTGAAGGAAGCTCACGCCAATCGGATGGGGAGCCCGAAGAAGCGAGTTTCGATCCCAGATAAACCAAAGGAGGAAGATTACTTCTACTCAAATCCACATGAATGTTTGCAGCTGTTAGATGAACCCTTCAGAACCAAAGGATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

505

Amino Acids

57.9

Weight (kDa)

9.27

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 108 - 422 2e-106 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1187
AccBSI CCGCTC 1 cut(s) 365
AccI GTMKAC 2 cut(s) 306, 558
AciI CCGC 3 cut(s) 161, 365, 1059
AclWI GGATC 2 cut(s) 604, 1414
AcsI RAATTY 2 cut(s) 934, 1099
AcuI CTGAAG 1 cut(s) 1480
AcyI GRCGYC 2 cut(s) 155, 217
AfaI GTAC 2 cut(s) 642, 1223
AfiI CCNNNNNNNGG 3 cut(s) 619, 751, 802
AflIII ACRYGT 1 cut(s) 588
AgeI ACCGGT 1 cut(s) 571
AloI GAACNNNNNNTCC 2 cut(s) 1145, 1177
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 2 cut(s) 1109, 1195
AlwI GGATC 2 cut(s) 604, 1414
AlwNI CAGNNNCTG 2 cut(s) 613, 703
Ama87I CYCGRG 2 cut(s) 422, 663
AoxI GGCC 4 cut(s) 15, 194, 793, 1252
ApaI GGGCCC 1 cut(s) 797
ApoI RAATTY 2 cut(s) 934, 1099
AseI ATTAAT 1 cut(s) 1316
AsiGI ACCGGT 1 cut(s) 571
AspLEI GCGC 1 cut(s) 708
AspS9I GGNCC 3 cut(s) 194, 793, 794
AsuC2I CCSGG 2 cut(s) 128, 713
AsuHPI GGTGA 1 cut(s) 880
AsuII TTCGAA 1 cut(s) 514
AvaI CYCGRG 2 cut(s) 422, 663
BaeGI GKGCMC 2 cut(s) 797, 968
BanII GRGCYC 3 cut(s) 95, 797, 1403
BarI GAAGNNNNNNTAC 2 cut(s) 17, 49
Bbv12I GWGCWC 1 cut(s) 95
BbvCI CCTCAGC 1 cut(s) 12
BccI CCATC 2 cut(s) 1220, 1387
BceAI ACGGC 1 cut(s) 236
BclI TGATCA 3 cut(s) 120, 481, 1015
BcnI CCSGG 2 cut(s) 128, 713
BcoDI GTCTC 2 cut(s) 1109, 1195
BfaI CTAG 4 cut(s) 198, 428, 953, 1187
BfmI CTRYAG 1 cut(s) 850
Bme1390I CCNGG 2 cut(s) 128, 713
BmeT110I CYCGRG 2 cut(s) 422, 663
BmgT120I GGNCC 3 cut(s) 194, 793, 794
BmiI GGNNCC 4 cut(s) 195, 795, 1128, 1400
BmrFI CCNGG 2 cut(s) 128, 713
BmsI GCATC 2 cut(s) 649, 1062
BpmI CTGGAG 2 cut(s) 1065, 1145
Bpu10I CCTNAGC 2 cut(s) 12, 542
Bpu14I TTCGAA 1 cut(s) 514
BpuEI CTTGAG 1 cut(s) 710
BpuMI CCSGG 2 cut(s) 128, 713
BsaAI YACGTR 1 cut(s) 1221
BsaBI GATNNNNATC 2 cut(s) 480, 1034
BsaHI GRCGYC 2 cut(s) 155, 217
BsaWI WCCGGW 1 cut(s) 571
Bsc4I CCNNNNNNNGG 3 cut(s) 619, 751, 802
Bse118I RCCGGY 1 cut(s) 571
Bse1I ACTGG 1 cut(s) 1339
Bse3DI GCAATG 3 cut(s) 51, 346, 874
Bse8I GATNNNNATC 2 cut(s) 480, 1034
BseGI GGATG 4 cut(s) 123, 1077, 1357, 1398
BseJI GATNNNNATC 2 cut(s) 480, 1034
BseLI CCNNNNNNNGG 3 cut(s) 619, 751, 802
BseMI GCAATG 3 cut(s) 51, 346, 874
BseMII CTCAG 5 cut(s) 108, 242, 556, 722, 787
BseNI ACTGG 1 cut(s) 1339
BseSI GKGCMC 2 cut(s) 797, 968
BseYI CCCAGC 1 cut(s) 1347
BsgI GTGCAG 2 cut(s) 185, 644
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 4 cut(s) 17, 196, 795, 1254
BshTI ACCGGT 1 cut(s) 571
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 95
BsiHKCI CYCGRG 2 cut(s) 422, 663
BsiSI CCGG 3 cut(s) 127, 572, 712
BsiWI CGTACG 1 cut(s) 1221
BslI CCNNNNNNNGG 3 cut(s) 619, 751, 802
BsmAI GTCTC 2 cut(s) 1109, 1195
BsmBI CGTCTC 1 cut(s) 1109
BsmI GAATGC 1 cut(s) 839
BsnI GGCC 4 cut(s) 17, 196, 795, 1254
BsoBI CYCGRG 2 cut(s) 422, 663
Bsp119I TTCGAA 1 cut(s) 514
Bsp120I GGGCCC 1 cut(s) 793
Bsp1286I GDGCHC 4 cut(s) 95, 797, 968, 1403
Bsp143I GATC 8 cut(s) 120, 132, 481, 609, 921, 1015, 1419, 1508
BspACI CCGC 3 cut(s) 161, 365, 1059
BspANI GGCC 4 cut(s) 17, 196, 795, 1254
BspCNI CTCAG 6 cut(s) 4, 107, 241, 555, 723, 786
BspLI GGNNCC 4 cut(s) 195, 795, 1128, 1400
BspMAI CTGCAG 1 cut(s) 854
BspPI GGATC 2 cut(s) 604, 1414
BspQI GCTCTTC 2 cut(s) 83, 813
BspT104I TTCGAA 1 cut(s) 514
BsrBI CCGCTC 1 cut(s) 365
BsrDI GCAATG 3 cut(s) 51, 346, 874
BsrFI RCCGGY 1 cut(s) 571
BsrI ACTGG 1 cut(s) 1339
BssAI RCCGGY 1 cut(s) 571
BssMI GATC 8 cut(s) 120, 132, 481, 609, 921, 1015, 1419, 1508
BssNAI GTATAC 1 cut(s) 559
BssNI GRCGYC 2 cut(s) 155, 217
Bst1107I GTATAC 1 cut(s) 559
Bst4CI ACNGT 2 cut(s) 556, 1285
Bst6I CTCTTC 3 cut(s) 83, 813, 1165
BstACI GRCGYC 2 cut(s) 155, 217
BstBAI YACGTR 1 cut(s) 1221
BstBI TTCGAA 1 cut(s) 514
BstC8I GCNNGC 1 cut(s) 363
BstDEI CTNAG 8 cut(s) 12, 94, 228, 542, 731, 773, 1195, 1512
BstF5I GGATG 4 cut(s) 123, 1077, 1357, 1398
BstHHI GCGC 1 cut(s) 708
BstKTI GATC 8 cut(s) 123, 135, 484, 612, 924, 1018, 1422, 1511
BstMAI GTCTC 2 cut(s) 1109, 1195
BstMBI GATC 8 cut(s) 120, 132, 481, 609, 921, 1015, 1419, 1508
BstMCI CGRYCG 1 cut(s) 135
BstMWI GCNNNNNNNGC 6 cut(s) 277, 538, 622, 668, 1126, 1344
BstNSI RCATGY 1 cut(s) 592
BstSCI CCNGG 2 cut(s) 126, 711
BstSFI CTRYAG 1 cut(s) 850
BstSLI GKGCMC 2 cut(s) 797, 968
BstSNI TACGTA 1 cut(s) 1221
BstX2I RGATCY 2 cut(s) 609, 1508
BstXI CCANNNNNNTGG 1 cut(s) 1393
BstYI RGATCY 2 cut(s) 609, 1508
BstZ17I GTATAC 1 cut(s) 559
BsuRI GGCC 4 cut(s) 17, 196, 795, 1254
BtsCI GGATG 4 cut(s) 123, 1077, 1357, 1398
BtsIMutI CAGTG 2 cut(s) 102, 1332
Cac8I GCNNGC 1 cut(s) 363
CaiI CAGNNNCTG 2 cut(s) 613, 703
CfoI GCGC 1 cut(s) 708
Cfr10I RCCGGY 1 cut(s) 571
Cfr13I GGNCC 3 cut(s) 194, 793, 794
CseI GACGC 3 cut(s) 144, 206, 1126
Csp6I GTAC 2 cut(s) 641, 1222
CspAI ACCGGT 1 cut(s) 571
CspCI CAANNNNNGTGG 2 cut(s) 33, 68
CviAII CATG 8 cut(s) 111, 248, 328, 589, 725, 857, 1234, 1466
CviQI GTAC 2 cut(s) 641, 1222
DdeI CTNAG 8 cut(s) 12, 94, 228, 542, 731, 773, 1195, 1512
DpnI GATC 8 cut(s) 122, 134, 483, 611, 923, 1017, 1421, 1510
DpnII GATC 8 cut(s) 120, 132, 481, 609, 921, 1015, 1419, 1508
DraI TTTAAA 1 cut(s) 1083
DrdI GACNNNNNNGTC 1 cut(s) 1187
DseDI GACNNNNNNGTC 1 cut(s) 1187
Eam1104I CTCTTC 3 cut(s) 83, 813, 1165
EarI CTCTTC 3 cut(s) 83, 813, 1165
EciI GGCGGA 1 cut(s) 1074
Ecl136II GAGCTC 1 cut(s) 93
Eco105I TACGTA 1 cut(s) 1221
Eco147I AGGCCT 1 cut(s) 17
Eco24I GRGCYC 3 cut(s) 95, 797, 1403
Eco53kI GAGCTC 1 cut(s) 93
Eco57I CTGAAG 1 cut(s) 1480
Eco88I CYCGRG 2 cut(s) 422, 663
EcoICRI GAGCTC 1 cut(s) 93
EcoO109I RGGNCCY 3 cut(s) 194, 793, 794
EcoT38I GRGCYC 3 cut(s) 95, 797, 1403
Esp3I CGTCTC 1 cut(s) 1109
FaeI CATG 8 cut(s) 114, 251, 331, 592, 728, 860, 1237, 1469
FatI CATG 8 cut(s) 110, 247, 327, 588, 724, 856, 1233, 1465
FbaI TGATCA 3 cut(s) 120, 481, 1015
FblI GTMKAC 2 cut(s) 306, 558
FokI GGATG 4 cut(s) 110, 1084, 1364, 1405
FriOI GRGCYC 3 cut(s) 95, 797, 1403
FspBI CTAG 4 cut(s) 198, 428, 953, 1187
GlaI GCGC 1 cut(s) 707
GsaI CCCAGC 1 cut(s) 1351
GsuI CTGGAG 2 cut(s) 1065, 1145
HaeIII GGCC 4 cut(s) 17, 196, 795, 1254
HapII CCGG 3 cut(s) 127, 572, 712
HgaI GACGC 3 cut(s) 144, 206, 1126
HhaI GCGC 1 cut(s) 708
Hin1I GRCGYC 2 cut(s) 155, 217
Hin1II CATG 8 cut(s) 114, 251, 331, 592, 728, 860, 1237, 1469
Hin6I GCGC 1 cut(s) 706
HinP1I GCGC 1 cut(s) 706
HindIII AAGCTT 2 cut(s) 269, 767
HinfI GANTC 3 cut(s) 352, 735, 1263
HpaII CCGG 3 cut(s) 127, 572, 712
HphI GGTGA 1 cut(s) 880
Hpy166II GTNNAC 2 cut(s) 307, 559
Hpy188I TCNGA 9 cut(s) 609, 732, 818, 940, 1098, 1109, 1135, 1392, 1499
Hpy188III TCNNGA 9 cut(s) 422, 464, 544, 689, 697, 775, 1027, 1044, 1150
Hpy8I GTNNAC 2 cut(s) 307, 559
Hpy99I CGWCG 1 cut(s) 160
HpyAV CCTTC 9 cut(s) 43, 611, 950, 1062, 1140, 1244, 1293, 1366, 1504
HpyCH4III ACNGT 2 cut(s) 556, 1285
HpyCH4IV ACGT 2 cut(s) 1023, 1220
HpyF10VI GCNNNNNNNGC 6 cut(s) 277, 538, 622, 668, 1126, 1344
HpyF3I CTNAG 8 cut(s) 12, 94, 228, 542, 731, 773, 1195, 1512
HpySE526I ACGT 2 cut(s) 1023, 1220
Hsp92I GRCGYC 2 cut(s) 155, 217
Hsp92II CATG 8 cut(s) 114, 251, 331, 592, 728, 860, 1237, 1469
HspAI GCGC 1 cut(s) 706
Ksp22I TGATCA 3 cut(s) 120, 481, 1015
Kzo9I GATC 8 cut(s) 120, 132, 481, 609, 921, 1015, 1419, 1508
LguI GCTCTTC 2 cut(s) 83, 813
LmnI GCTCC 2 cut(s) 1126, 1398
LweI GCATC 2 cut(s) 649, 1062
MaeI CTAG 4 cut(s) 198, 428, 953, 1187
MaeII ACGT 2 cut(s) 1023, 1220
MaeIII GTNAC 2 cut(s) 136, 406
MalI GATC 8 cut(s) 122, 134, 483, 611, 923, 1017, 1421, 1510
MbiI CCGCTC 1 cut(s) 365
MboI GATC 8 cut(s) 120, 132, 481, 609, 921, 1015, 1419, 1508
MboII GAAGA 9 cut(s) 100, 115, 800, 890, 893, 931, 1152, 1417, 1454
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 2 cut(s) 609, 1508
MhlI GDGCHC 4 cut(s) 95, 797, 968, 1403
MluCI AATT 9 cut(s) 31, 257, 282, 527, 718, 934, 1011, 1099, 1325
MmeI TCCRAC 2 cut(s) 213, 1158
MseI TTAA 6 cut(s) 30, 60, 521, 717, 1082, 1316
MslI CAYNNNNRTG 2 cut(s) 893, 1468
MspA1I CMGCKG 2 cut(s) 169, 1480
MspI CCGG 3 cut(s) 127, 572, 712
MspR9I CCNGG 2 cut(s) 128, 713
MunI CAATTG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 839
MwoI GCNNNNNNNGC 6 cut(s) 277, 538, 622, 668, 1126, 1344
NciI CCSGG 2 cut(s) 128, 713
NdeII GATC 8 cut(s) 120, 132, 481, 609, 921, 1015, 1419, 1508
NlaIII CATG 8 cut(s) 114, 251, 331, 592, 728, 860, 1237, 1469
NlaIV GGNNCC 4 cut(s) 195, 795, 1128, 1400
NmuCI GTSAC 1 cut(s) 406
NspI RCATGY 1 cut(s) 592
NspV TTCGAA 1 cut(s) 514
PaeR7I CTCGAG 1 cut(s) 663
PceI AGGCCT 1 cut(s) 17
PciI ACATGT 1 cut(s) 588
PciSI GCTCTTC 2 cut(s) 83, 813
PctI GAATGC 1 cut(s) 839
PfeI GAWTC 3 cut(s) 352, 735, 1263
Pfl23II CGTACG 1 cut(s) 1221
PinAI ACCGGT 1 cut(s) 571
Ple19I CGATCG 1 cut(s) 135
Ppu21I YACGTR 1 cut(s) 1221
PscI ACATGT 1 cut(s) 588
PshBI ATTAAT 1 cut(s) 1316
Psp124BI GAGCTC 1 cut(s) 95
PspFI CCCAGC 1 cut(s) 1347
PspLI CGTACG 1 cut(s) 1221
PspN4I GGNNCC 4 cut(s) 195, 795, 1128, 1400
PspOMI GGGCCC 1 cut(s) 793
PspPI GGNCC 3 cut(s) 194, 793, 794
PspXI VCTCGAGB 1 cut(s) 663
PstI CTGCAG 1 cut(s) 854
PstNI CAGNNNCTG 2 cut(s) 613, 703
PsuI RGATCY 2 cut(s) 609, 1508
PvuI CGATCG 1 cut(s) 135
PvuII CAGCTG 2 cut(s) 169, 1480
RsaI GTAC 2 cut(s) 642, 1223
RsaNI GTAC 2 cut(s) 641, 1222
RseI CAYNNNNRTG 2 cut(s) 893, 1468
SacI GAGCTC 1 cut(s) 95
SapI GCTCTTC 2 cut(s) 83, 813
SaqAI TTAA 6 cut(s) 30, 60, 521, 717, 1082, 1316
Sau3AI GATC 8 cut(s) 120, 132, 481, 609, 921, 1015, 1419, 1508
Sau96I GGNCC 3 cut(s) 194, 793, 794
ScrFI CCNGG 2 cut(s) 128, 713
SduI GDGCHC 4 cut(s) 95, 797, 968, 1403
SfaNI GCATC 2 cut(s) 649, 1062
SfcI CTRYAG 1 cut(s) 850
Sfr274I CTCGAG 1 cut(s) 663
SfuI TTCGAA 1 cut(s) 514
SlaI CTCGAG 1 cut(s) 663
SmiMI CAYNNNNRTG 2 cut(s) 893, 1468
SmlI CTYRAG 2 cut(s) 663, 689
SmoI CTYRAG 2 cut(s) 663, 689
SnaBI TACGTA 1 cut(s) 1221
Sse9I AATT 9 cut(s) 31, 257, 282, 527, 718, 934, 1011, 1099, 1325
SseBI AGGCCT 1 cut(s) 17
SsiI CCGC 3 cut(s) 161, 365, 1059
SspI AATATT 1 cut(s) 519
SspMI CTAG 4 cut(s) 198, 428, 953, 1187
SstI GAGCTC 1 cut(s) 95
StuI AGGCCT 1 cut(s) 17
StyD4I CCNGG 2 cut(s) 126, 711
TaaI ACNGT 2 cut(s) 556, 1285
TaiI ACGT 2 cut(s) 1026, 1223
TaqI TCGA 3 cut(s) 514, 664, 1418
TasI AATT 9 cut(s) 31, 257, 282, 527, 718, 934, 1011, 1099, 1325
TatI WGTACW 1 cut(s) 640
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 3 cut(s) 352, 735, 1263
Tru1I TTAA 6 cut(s) 30, 60, 521, 717, 1082, 1316
Tru9I TTAA 6 cut(s) 30, 60, 521, 717, 1082, 1316
TscAI CASTG 2 cut(s) 102, 1339
TseFI GTSAC 1 cut(s) 406
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 7 cut(s) 62, 236, 478, 593, 800, 1482, 1503
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 2 cut(s) 102, 1339
VspI ATTAAT 1 cut(s) 1316
XapI RAATTY 2 cut(s) 934, 1099
XceI RCATGY 1 cut(s) 592
XcmI CCANNNNNNNNNTGG 1 cut(s) 576
XhoI CTCGAG 1 cut(s) 663
XmiI GTMKAC 2 cut(s) 306, 558
XspI CTAG 4 cut(s) 198, 428, 953, 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.