Rh2BG589300

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002611
Physical Location & Seq
Reverse (-)
80588477 .. 80591658
3182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG589300.1

Sequence Viewer

Length: 1167 bp
ATGTGCAAAGCAGGGGACTTTGAGAGGTGTGATTACAGAACAGAGTGGAAGATGGGATACAAGGTTTTGAGTAATGTAGAGAAATTGAAGAACTCAATTTTCAAGATGAACTCATGGTTGTTGATCATGAGAGCAATTGTGACAGCTTTGGTTTGGGCCTCTGCGGTTCAGTTGATTGGGATTTGGTTGATTAGAGGGCCAAGAGTGTTCAAGGTTTGGCCTCCTTGTAATCAATTAGATTTGCACGTTGCTTCTGAATTGCCGAAGCTTCCAATCCCAGTTTTCCCACCAAAGAGGGTTTATAAGAATAATGGGTATCTAATGGTTTCTTGCAATGGAGGATTGAATCAAATGCGAGCAGCGATTTGTGATATGGTTGCAATTGCCAGATATTTGAATGTGACTCTTATCGTTCCAGAGCTGGATAAGAACTCATTTTGGGCTGCGAGTGAGTTTCAAGACATTTTCGATGTTAATCATTTCATTAGATCATTGAGAGATGAGGTTCGGATACTAAAGAAACTGCCTCCAAAGCTCAACAAGAGAGCAGTATACTCATTGCCACCTGTTAGTTGGTCCAACATTTCCTACTACATTCATCAGATCCTACCGCTGGTGCGAAAGTATAAAGTAGTGCATTTGAACAAAACCGATGCTCGAGTGGCTAACAATGGGCTACCCCTTGAGATTCAGAAGTTGAGATGCAGAGTCAATTTCCATGGTCTGAGATTTACTTCCCAGATAGAGGAAACCGGTCGAAAGCTTGTCAGAATTTTAAGAGAAAAAGGCCCTTTTCTGGTGCTTCATCTCAGATATGAAATGGACATGTTGGCATTCTCTGGCTGCAGCCATGGTTGCACCAATGATGAGGTGGAAGAACTCAACACAATGAGATATGCTAATCCATTGTGGAAGGAAAAAGACATTGATTCTGAAATGAAAAGGGTAGAAGGCTTGTGCCCTCTAACACCAGAGGAAACTGCTCTGGTATTGGTTGCATTAGGTATAGATCACAATGTTCAGATTTATATTGCTGCCGGAGACATATACGGTGGAGATCGAAGAATGGAAGGTTTGAAAGCTGCTTTTCCTAATGTGGTAAGTAGAATTAATGAAGCAGAAGCAACTTTTCCAACAACTATACATAGGTCTCTCGCTCTCGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

388

Amino Acids

44.41

Weight (kDa)

9.28

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 106 - 371 1.4e-84 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AccI GTMKAC 1 cut(s) 552
AciI CCGC 2 cut(s) 164, 611
AclWI GGATC 1 cut(s) 598
AcsI RAATTY 1 cut(s) 771
AfiI CCNNNNNNNGG 3 cut(s) 613, 745, 796
AflIII ACRYGT 1 cut(s) 825
AgeI ACCGGT 1 cut(s) 752
AgsI TTSAA 8 cut(s) 88, 103, 211, 346, 397, 458, 643, 1078
AluBI AGCT 6 cut(s) 146, 268, 421, 535, 763, 1082
AluI AGCT 6 cut(s) 146, 268, 421, 535, 763, 1082
Alw26I GTCTC 2 cut(s) 1035, 1155
AlwI GGATC 1 cut(s) 598
Ama87I CYCGRG 1 cut(s) 657
AoxI GGCC 4 cut(s) 156, 197, 218, 787
ApeKI GCWGC 6 cut(s) 359, 443, 843, 846, 1034, 1082
ApoI RAATTY 1 cut(s) 771
AseI ATTAAT 1 cut(s) 1110
AsiGI ACCGGT 1 cut(s) 752
AspS9I GGNCC 4 cut(s) 156, 197, 576, 788
AvaI CYCGRG 1 cut(s) 657
AvaII GGWCC 1 cut(s) 576
BaeGI GKGCMC 1 cut(s) 962
BarI GAAGNNNNNNTAC 2 cut(s) 41, 73
BbvI GCAGC 6 cut(s) 371, 430, 830, 858, 1021, 1069
BccI CCATC 1 cut(s) 46
BciVI GTATCC 2 cut(s) 50, 504
BclI TGATCA 1 cut(s) 123
BcoDI GTCTC 2 cut(s) 1035, 1155
BfmI CTRYAG 1 cut(s) 844
BfuI GTATCC 2 cut(s) 50, 504
BisI GCNGC 6 cut(s) 360, 444, 844, 847, 1035, 1083
BlsI GCNGC 6 cut(s) 361, 445, 845, 848, 1036, 1084
Bme18I GGWCC 1 cut(s) 576
BmeT110I CYCGRG 1 cut(s) 657
BmgT120I GGNCC 4 cut(s) 156, 197, 576, 788
BmrI ACTGGG 1 cut(s) 272
BmsI GCATC 2 cut(s) 643, 692
BmuI ACTGGG 1 cut(s) 272
BpuEI CTTGAG 1 cut(s) 704
BsaBI GATNNNNATC 1 cut(s) 474
BsaI GGTCTC 1 cut(s) 1155
BsaJI CCNNGG 2 cut(s) 718, 850
BsaWI WCCGGW 1 cut(s) 752
Bsc4I CCNNNNNNNGG 3 cut(s) 613, 745, 796
Bse118I RCCGGY 1 cut(s) 752
Bse1I ACTGG 1 cut(s) 278
Bse3DI GCAATG 2 cut(s) 340, 557
Bse8I GATNNNNATC 1 cut(s) 474
BseDI CCNNGG 2 cut(s) 718, 850
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 3 cut(s) 613, 745, 796
BseMI GCAATG 2 cut(s) 340, 557
BseMII CTCAG 2 cut(s) 716, 823
BseNI ACTGG 1 cut(s) 278
BseSI GKGCMC 1 cut(s) 962
BseXI GCAGC 6 cut(s) 371, 430, 830, 858, 1021, 1069
Bsh1285I CGRYCG 1 cut(s) 757
BshFI GGCC 4 cut(s) 158, 199, 220, 789
BshTI ACCGGT 1 cut(s) 752
BsiEI CGRYCG 1 cut(s) 757
BsiHKCI CYCGRG 1 cut(s) 657
BsiSI CCGG 2 cut(s) 753, 1038
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 3 cut(s) 613, 745, 796
BsmAI GTCTC 2 cut(s) 1035, 1155
BsmFI GGGAC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 833
BsnI GGCC 4 cut(s) 158, 199, 220, 789
Bso31I GGTCTC 1 cut(s) 1155
BsoBI CYCGRG 1 cut(s) 657
Bsp1286I GDGCHC 1 cut(s) 962
Bsp143I GATC 5 cut(s) 123, 488, 603, 1009, 1057
Bsp19I CCATGG 2 cut(s) 718, 850
BspACI CCGC 2 cut(s) 164, 611
BspANI GGCC 4 cut(s) 158, 199, 220, 789
BspCNI CTCAG 2 cut(s) 717, 822
BspHI TCATGA 1 cut(s) 126
BspMAI CTGCAG 1 cut(s) 848
BspPI GGATC 1 cut(s) 598
BspTNI GGTCTC 1 cut(s) 1155
BsrDI GCAATG 2 cut(s) 340, 557
BsrFI RCCGGY 1 cut(s) 752
BsrI ACTGG 1 cut(s) 278
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 2 cut(s) 718, 850
BssMI GATC 5 cut(s) 123, 488, 603, 1009, 1057
BssNAI GTATAC 1 cut(s) 553
BssT1I CCWWGG 2 cut(s) 718, 850
Bst1107I GTATAC 1 cut(s) 553
Bst4CI ACNGT 1 cut(s) 1052
BstC8I GCNNGC 1 cut(s) 357
BstDEI CTNAG 2 cut(s) 725, 809
BstDSI CCRYGG 2 cut(s) 718, 850
BstKTI GATC 5 cut(s) 126, 491, 606, 1012, 1060
BstMAI GTCTC 2 cut(s) 1035, 1155
BstMBI GATC 5 cut(s) 123, 488, 603, 1009, 1057
BstMCI CGRYCG 1 cut(s) 757
BstMWI GCNNNNNNNGC 3 cut(s) 532, 662, 855
BstNSI RCATGY 1 cut(s) 829
BstSFI CTRYAG 1 cut(s) 844
BstSLI GKGCMC 1 cut(s) 962
BstV1I GCAGC 6 cut(s) 371, 430, 830, 858, 1021, 1069
BstX2I RGATCY 1 cut(s) 603
BstYI RGATCY 1 cut(s) 603
BstZ17I GTATAC 1 cut(s) 553
BsuI GTATCC 2 cut(s) 50, 504
BsuRI GGCC 4 cut(s) 158, 199, 220, 789
BtgI CCRYGG 2 cut(s) 718, 850
Cac8I GCNNGC 1 cut(s) 357
CciI TCATGA 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 752
Cfr13I GGNCC 4 cut(s) 156, 197, 576, 788
CspAI ACCGGT 1 cut(s) 752
CviAII CATG 5 cut(s) 114, 127, 719, 826, 851
DdeI CTNAG 2 cut(s) 725, 809
DpnI GATC 5 cut(s) 125, 490, 605, 1011, 1059
DpnII GATC 5 cut(s) 123, 488, 603, 1009, 1057
Eco130I CCWWGG 2 cut(s) 718, 850
Eco31I GGTCTC 1 cut(s) 1155
Eco47I GGWCC 1 cut(s) 576
Eco88I CYCGRG 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 788
EcoT14I CCWWGG 2 cut(s) 718, 850
ErhI CCWWGG 2 cut(s) 718, 850
FaeI CATG 5 cut(s) 117, 130, 722, 829, 854
FaqI GGGAC 1 cut(s) 29
FatI CATG 5 cut(s) 113, 126, 718, 825, 850
FbaI TGATCA 1 cut(s) 123
FblI GTMKAC 1 cut(s) 552
Fnu4HI GCNGC 6 cut(s) 360, 444, 844, 847, 1035, 1083
Fsp4HI GCNGC 6 cut(s) 360, 444, 844, 847, 1035, 1083
GluI GCNGC 6 cut(s) 360, 444, 844, 847, 1035, 1083
HaeIII GGCC 4 cut(s) 158, 199, 220, 789
HapII CCGG 2 cut(s) 753, 1038
Hin1II CATG 5 cut(s) 117, 130, 722, 829, 854
HindIII AAGCTT 2 cut(s) 266, 761
HinfI GANTC 5 cut(s) 346, 403, 688, 708, 929
HpaII CCGG 2 cut(s) 753, 1038
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 9 cut(s) 256, 510, 603, 693, 726, 770, 812, 934, 1023
Hpy188III TCNNGA 4 cut(s) 103, 127, 416, 458
Hpy8I GTNNAC 1 cut(s) 553
HpyAV CCTTC 3 cut(s) 907, 944, 1064
HpyCH4III ACNGT 1 cut(s) 1052
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 9 cut(s) 6, 244, 333, 380, 637, 705, 846, 858, 998
HpyF10VI GCNNNNNNNGC 3 cut(s) 532, 662, 855
HpyF3I CTNAG 2 cut(s) 725, 809
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 5 cut(s) 117, 130, 722, 829, 854
Ksp22I TGATCA 1 cut(s) 123
Kzo9I GATC 5 cut(s) 123, 488, 603, 1009, 1057
Lsp1109I GCAGC 6 cut(s) 371, 430, 830, 858, 1021, 1069
LweI GCATC 2 cut(s) 643, 692
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 2 cut(s) 139, 400
MalI GATC 5 cut(s) 125, 490, 605, 1011, 1059
MboI GATC 5 cut(s) 123, 488, 603, 1009, 1057
MboII GAAGA 4 cut(s) 61, 100, 887, 1074
MfeI CAATTG 2 cut(s) 135, 381
MflI RGATCY 1 cut(s) 603
MhlI GDGCHC 1 cut(s) 962
MluCI AATT 9 cut(s) 83, 96, 135, 233, 257, 381, 712, 771, 1107
MlyI GAGTC 2 cut(s) 397, 717
MmeI TCCRAC 2 cut(s) 603, 1157
MseI TTAA 3 cut(s) 474, 776, 1110
MspA1I CMGCKG 1 cut(s) 613
MspI CCGG 2 cut(s) 753, 1038
MunI CAATTG 2 cut(s) 135, 381
Mva1269I GAATGC 1 cut(s) 833
MwoI GCNNNNNNNGC 3 cut(s) 532, 662, 855
NcoI CCATGG 2 cut(s) 718, 850
NdeII GATC 5 cut(s) 123, 488, 603, 1009, 1057
NlaIII CATG 5 cut(s) 117, 130, 722, 829, 854
NmuCI GTSAC 2 cut(s) 139, 400
NspI RCATGY 1 cut(s) 829
PaeR7I CTCGAG 1 cut(s) 657
PagI TCATGA 1 cut(s) 126
PciI ACATGT 1 cut(s) 825
PctI GAATGC 1 cut(s) 833
PfeI GAWTC 3 cut(s) 346, 688, 929
PinAI ACCGGT 1 cut(s) 752
PkrI GCNGC 6 cut(s) 361, 445, 845, 848, 1036, 1084
PleI GAGTC 2 cut(s) 397, 716
PpsI GAGTC 2 cut(s) 397, 716
PscI ACATGT 1 cut(s) 825
PshBI ATTAAT 1 cut(s) 1110
PsiI TTATAA 1 cut(s) 303
PspPI GGNCC 4 cut(s) 156, 197, 576, 788
PspXI VCTCGAGB 1 cut(s) 657
PstI CTGCAG 1 cut(s) 848
PsuI RGATCY 1 cut(s) 603
SaqAI TTAA 3 cut(s) 474, 776, 1110
SatI GCNGC 6 cut(s) 360, 444, 844, 847, 1035, 1083
Sau3AI GATC 5 cut(s) 123, 488, 603, 1009, 1057
Sau96I GGNCC 4 cut(s) 156, 197, 576, 788
SchI GAGTC 2 cut(s) 397, 717
SduI GDGCHC 1 cut(s) 962
SfaNI GCATC 2 cut(s) 643, 692
SfcI CTRYAG 1 cut(s) 844
Sfr274I CTCGAG 1 cut(s) 657
SinI GGWCC 1 cut(s) 576
SlaI CTCGAG 1 cut(s) 657
SmlI CTYRAG 2 cut(s) 657, 683
SmoI CTYRAG 2 cut(s) 657, 683
Sse9I AATT 9 cut(s) 83, 96, 135, 233, 257, 381, 712, 771, 1107
SsiI CCGC 2 cut(s) 164, 611
StyI CCWWGG 2 cut(s) 718, 850
TaaI ACNGT 1 cut(s) 1052
TaiI ACGT 1 cut(s) 249
TaqI TCGA 4 cut(s) 468, 658, 757, 1060
TasI AATT 9 cut(s) 83, 96, 135, 233, 257, 381, 712, 771, 1107
TfiI GAWTC 3 cut(s) 346, 688, 929
Tru1I TTAA 3 cut(s) 474, 776, 1110
Tru9I TTAA 3 cut(s) 474, 776, 1110
TseFI GTSAC 2 cut(s) 139, 400
TseI GCWGC 6 cut(s) 359, 443, 843, 846, 1034, 1082
Tsp45I GTSAC 2 cut(s) 139, 400
TspDTI ATGAA 7 cut(s) 122, 472, 587, 794, 831, 953, 1128
VpaK11BI GGWCC 1 cut(s) 576
VspI ATTAAT 1 cut(s) 1110
XapI RAATTY 1 cut(s) 771
XceI RCATGY 1 cut(s) 829
XcmI CCANNNNNNNNNTGG 2 cut(s) 570, 868
XhoI CTCGAG 1 cut(s) 657
XmiI GTMKAC 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.