pycom09g00320

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002611
Physical Location & Seq
Forward (+)
275564 .. 278017
2454 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g00320.1

Sequence Viewer

Length: 1518 bp
ATGTGCAAAGCTGAGGACTTTGAGAAGTTTAATTACAGAGAAGCAATGAAGGTGGGATTTAAGTTGATGGGGAGAGTTAAAAGGTTGAAGAGCTCAATGTTGAAGATGAACTCATGGCTGCTGATCATCAGGGCGATCGTTACAGCGTTGGTGTGGGCGTCGGCGGTGCAGCTGGTTGGGATTTGGCTGATTAAGGGGCCTAGTTTGTTCAATGTTTGGCGTCCTTGCACTCAGTTGGAATTGCCTGTTCATGCTGCTGAATTGGCTAACAAGCTTCCAATGCCAATTGTTCTCCCACCAAAGAGGGTCTACAAAAACAATGGCTATCTCATGGTTTCTTGCAATGGAGGATTGAATCAAATGCGAGCGGCAATCTGTGATATGGTTGCAGTTGCCAGATACTTGAATGTCACACTTATAGTTCCCGAGCTAGATAAAAACTCCTTTTGGGCTGCAAGTGAGTTTCAGGACATTTTTGATGTTGATCATTTCATTACATCCTTGAGAGACGAGGTTCGGATATTAAGGAAATTGCCACCGAAGCTCAGGAAAAGAGCTGTATACTCAATGCCACCAGTTAGTTGGTCTAACATGTCCTACTACCTTCATCAGATCCTGCCTCTGGTGCAGAAGTATAAAGTAGTACATTTGAATAAAACTGATGCTCGAGTAGCTAACAATGGGCTACCTCTTGAGTTCCAGAAGCTGCGCTGCCGAGTTAATTTCCATGCTCTGAGATTCACTTCCCAGATAGAGGAAATCGGTAGAAAGCTTCTCAGGATTTTGAGAGAAAAGGGCCCTTTTCTGGCTCTTCATCTCCGATATGAAATGGATATGTTGGCATTCTCTGGCTGCAGTCATGGTTGCAGCAATGATGAGGAGGAAGAACTCACCAAAATGCGATATGCTAACCCCTTGTGGAAGATCAAAGACATAAATTCAGAAACAAAAAGGCTAGAAGGCTTGTGCCCCCTAACACCAGAGGAAACTGCTCTGGTGTTGGTTGCACTTGGAATTGATCACAACGTCCAGATTTATATCGCCTCTGGAGAAATGTATGGCGGAAACAGAAGGATGCAAGGTTTAAAAGCTGCTTTTCCGAATTTGGTCAGAAAAGAGACGCTGCTGGAGCCTTCGGATTTGAGGTTTTTCCCAAACCACTCTTCCCAAATGGCAGCGTTGGACTATCTAGTCTCCTTAGAGAGTGACATATTTATTCCTACGTACGATGGAAACATGGCTAAAGTTGTTGAAGGCCAACGCAGATTCTTGGGTTTCAAAAGGACAGTTTTATTAGACAGAAGGCTACTGATTGGATTAATAGAACAATTCAGCACCGGCTCATTGAGCTGGGATGAGTTCTCTTACATTGTGAAGGAAGCTCACGCCAATCGGATGGGGAGCCCAAAGAAGCGAGTTCCGATCCCAGATAAACCAAAGGAGGAAGATTACTTCTACTCAAATCCACATGAATGTTTGCATCTGTTAGATGAACCCTTGAGAACCACAGGATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

506

Amino Acids

58.01

Weight (kDa)

9.27

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 109 - 423 3.3e-106 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1190
AccBSI CCGCTC 1 cut(s) 368
AccI GTMKAC 2 cut(s) 309, 561
AciI CCGC 3 cut(s) 164, 368, 1062
AclWI GGATC 2 cut(s) 607, 1417
AcsI RAATTY 2 cut(s) 937, 1102
AcyI GRCGYC 2 cut(s) 158, 220
AfaI GTAC 2 cut(s) 645, 1226
AfiI CCNNNNNNNGG 3 cut(s) 622, 754, 805
AflIII ACRYGT 1 cut(s) 591
AgsI TTSAA 8 cut(s) 88, 103, 211, 355, 406, 652, 1253, 1279
AjuI GAANNNNNNNTTGG 2 cut(s) 1147, 1179
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 3 cut(s) 501, 1112, 1198
AlwI GGATC 2 cut(s) 607, 1417
AlwNI CAGNNNCTG 2 cut(s) 616, 706
Ama87I CYCGRG 2 cut(s) 425, 666
AoxI GGCC 3 cut(s) 197, 796, 1255
ApaI GGGCCC 1 cut(s) 800
ApoI RAATTY 2 cut(s) 937, 1102
AseI ATTAAT 1 cut(s) 1319
AspLEI GCGC 1 cut(s) 711
AspS9I GGNCC 3 cut(s) 197, 796, 797
AsuHPI GGTGA 1 cut(s) 883
AvaI CYCGRG 2 cut(s) 425, 666
BaeGI GKGCMC 2 cut(s) 800, 971
BanII GRGCYC 3 cut(s) 95, 800, 1406
BarI GAAGNNNNNNTAC 2 cut(s) 17, 49
Bbv12I GWGCWC 1 cut(s) 95
BbvCI CCTCAGC 1 cut(s) 12
BccI CCATC 3 cut(s) 61, 1223, 1390
BclI TGATCA 3 cut(s) 123, 484, 1018
BcoDI GTCTC 3 cut(s) 501, 1112, 1198
BfaI CTAG 4 cut(s) 201, 431, 956, 1190
BfmI CTRYAG 1 cut(s) 853
BmeT110I CYCGRG 2 cut(s) 425, 666
BmgT120I GGNCC 3 cut(s) 197, 796, 797
BmiI GGNNCC 4 cut(s) 198, 798, 1131, 1403
BmsI GCATC 3 cut(s) 652, 1065, 1489
BpmI CTGGAG 2 cut(s) 1068, 1148
Bpu10I CCTNAGC 2 cut(s) 12, 545
BpuEI CTTGAG 2 cut(s) 523, 713
BsaAI YACGTR 1 cut(s) 1224
BsaBI GATNNNNATC 2 cut(s) 483, 1037
BsaHI GRCGYC 2 cut(s) 158, 220
Bsc4I CCNNNNNNNGG 3 cut(s) 622, 754, 805
Bse118I RCCGGY 1 cut(s) 1337
Bse1I ACTGG 1 cut(s) 575
Bse3DI GCAATG 3 cut(s) 51, 349, 877
Bse8I GATNNNNATC 2 cut(s) 483, 1037
BseGI GGATG 4 cut(s) 497, 1080, 1360, 1401
BseJI GATNNNNATC 2 cut(s) 483, 1037
BseLI CCNNNNNNNGG 3 cut(s) 622, 754, 805
BseMI GCAATG 3 cut(s) 51, 349, 877
BseMII CTCAG 4 cut(s) 245, 559, 725, 790
BseNI ACTGG 1 cut(s) 575
BseRI GAGGAG 1 cut(s) 893
BseSI GKGCMC 2 cut(s) 800, 971
BseYI CCCAGC 1 cut(s) 1350
BsgI GTGCAG 2 cut(s) 188, 647
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 3 cut(s) 199, 798, 1257
BsiEI CGRYCG 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 95
BsiHKCI CYCGRG 2 cut(s) 425, 666
BsiSI CCGG 1 cut(s) 1338
BsiWI CGTACG 1 cut(s) 1224
BslI CCNNNNNNNGG 3 cut(s) 622, 754, 805
BsmAI GTCTC 3 cut(s) 501, 1112, 1198
BsmBI CGTCTC 2 cut(s) 501, 1112
BsmI GAATGC 1 cut(s) 842
BsnI GGCC 3 cut(s) 199, 798, 1257
BsoBI CYCGRG 2 cut(s) 425, 666
Bsp120I GGGCCC 1 cut(s) 796
Bsp1286I GDGCHC 4 cut(s) 95, 800, 971, 1406
Bsp143I GATC 8 cut(s) 123, 135, 484, 612, 924, 1018, 1422, 1511
BspACI CCGC 3 cut(s) 164, 368, 1062
BspANI GGCC 3 cut(s) 199, 798, 1257
BspCNI CTCAG 5 cut(s) 4, 244, 558, 726, 789
BspLI GGNNCC 4 cut(s) 198, 798, 1131, 1403
BspMAI CTGCAG 1 cut(s) 857
BspPI GGATC 2 cut(s) 607, 1417
BspQI GCTCTTC 2 cut(s) 83, 816
BsrBI CCGCTC 1 cut(s) 368
BsrDI GCAATG 3 cut(s) 51, 349, 877
BsrFI RCCGGY 1 cut(s) 1337
BsrI ACTGG 1 cut(s) 575
BssAI RCCGGY 1 cut(s) 1337
BssMI GATC 8 cut(s) 123, 135, 484, 612, 924, 1018, 1422, 1511
BssNAI GTATAC 1 cut(s) 562
BssNI GRCGYC 2 cut(s) 158, 220
Bst1107I GTATAC 1 cut(s) 562
Bst4CI ACNGT 1 cut(s) 1288
Bst6I CTCTTC 3 cut(s) 83, 816, 1168
BstACI GRCGYC 2 cut(s) 158, 220
BstBAI YACGTR 1 cut(s) 1224
BstC8I GCNNGC 1 cut(s) 366
BstDEI CTNAG 7 cut(s) 12, 231, 545, 734, 776, 1198, 1515
BstF5I GGATG 4 cut(s) 497, 1080, 1360, 1401
BstHHI GCGC 1 cut(s) 711
BstKTI GATC 8 cut(s) 126, 138, 487, 615, 927, 1021, 1425, 1514
BstMAI GTCTC 3 cut(s) 501, 1112, 1198
BstMBI GATC 8 cut(s) 123, 135, 484, 612, 924, 1018, 1422, 1511
BstMCI CGRYCG 1 cut(s) 138
BstMWI GCNNNNNNNGC 7 cut(s) 263, 280, 541, 625, 671, 1129, 1347
BstNSI RCATGY 1 cut(s) 595
BstSFI CTRYAG 1 cut(s) 853
BstSLI GKGCMC 2 cut(s) 800, 971
BstSNI TACGTA 1 cut(s) 1224
BstX2I RGATCY 2 cut(s) 612, 1511
BstXI CCANNNNNNTGG 2 cut(s) 582, 1396
BstYI RGATCY 2 cut(s) 612, 1511
BstZ17I GTATAC 1 cut(s) 562
BsuRI GGCC 3 cut(s) 199, 798, 1257
BtsCI GGATG 4 cut(s) 497, 1080, 1360, 1401
Cac8I GCNNGC 1 cut(s) 366
CaiI CAGNNNCTG 2 cut(s) 616, 706
CfoI GCGC 1 cut(s) 711
Cfr10I RCCGGY 1 cut(s) 1337
Cfr13I GGNCC 3 cut(s) 197, 796, 797
CseI GACGC 3 cut(s) 147, 209, 1129
Csp6I GTAC 2 cut(s) 644, 1225
CspCI CAANNNNNGTGG 2 cut(s) 33, 68
CviAII CATG 8 cut(s) 114, 251, 331, 592, 728, 860, 1237, 1469
CviQI GTAC 2 cut(s) 644, 1225
DdeI CTNAG 7 cut(s) 12, 231, 545, 734, 776, 1198, 1515
DpnI GATC 8 cut(s) 125, 137, 486, 614, 926, 1020, 1424, 1513
DpnII GATC 8 cut(s) 123, 135, 484, 612, 924, 1018, 1422, 1511
DraI TTTAAA 1 cut(s) 1086
DrdI GACNNNNNNGTC 1 cut(s) 1190
DseDI GACNNNNNNGTC 1 cut(s) 1190
Eam1104I CTCTTC 3 cut(s) 83, 816, 1168
EarI CTCTTC 3 cut(s) 83, 816, 1168
EciI GGCGGA 1 cut(s) 1077
Ecl136II GAGCTC 1 cut(s) 93
Eco105I TACGTA 1 cut(s) 1224
Eco24I GRGCYC 3 cut(s) 95, 800, 1406
Eco53kI GAGCTC 1 cut(s) 93
Eco88I CYCGRG 2 cut(s) 425, 666
EcoICRI GAGCTC 1 cut(s) 93
EcoO109I RGGNCCY 3 cut(s) 197, 796, 797
EcoT38I GRGCYC 3 cut(s) 95, 800, 1406
Esp3I CGTCTC 2 cut(s) 501, 1112
FaeI CATG 8 cut(s) 117, 254, 334, 595, 731, 863, 1240, 1472
FatI CATG 8 cut(s) 113, 250, 330, 591, 727, 859, 1236, 1468
FbaI TGATCA 3 cut(s) 123, 484, 1018
FblI GTMKAC 2 cut(s) 309, 561
FokI GGATG 4 cut(s) 484, 1087, 1367, 1408
FriOI GRGCYC 3 cut(s) 95, 800, 1406
FspBI CTAG 4 cut(s) 201, 431, 956, 1190
GlaI GCGC 1 cut(s) 710
GsaI CCCAGC 1 cut(s) 1354
GsuI CTGGAG 2 cut(s) 1068, 1148
HaeIII GGCC 3 cut(s) 199, 798, 1257
HapII CCGG 1 cut(s) 1338
HgaI GACGC 3 cut(s) 147, 209, 1129
HhaI GCGC 1 cut(s) 711
Hin1I GRCGYC 2 cut(s) 158, 220
Hin1II CATG 8 cut(s) 117, 254, 334, 595, 731, 863, 1240, 1472
Hin6I GCGC 1 cut(s) 709
HinP1I GCGC 1 cut(s) 709
HindIII AAGCTT 2 cut(s) 272, 770
HinfI GANTC 3 cut(s) 355, 738, 1266
HpaII CCGG 1 cut(s) 1338
HphI GGTGA 1 cut(s) 883
Hpy166II GTNNAC 2 cut(s) 310, 562
Hpy188III TCNNGA 8 cut(s) 425, 467, 547, 692, 700, 778, 1030, 1047
Hpy8I GTNNAC 2 cut(s) 310, 562
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 8 cut(s) 43, 614, 953, 1065, 1143, 1247, 1296, 1369
HpyCH4III ACNGT 1 cut(s) 1288
HpyCH4IV ACGT 2 cut(s) 1026, 1223
HpyF10VI GCNNNNNNNGC 7 cut(s) 263, 280, 541, 625, 671, 1129, 1347
HpyF3I CTNAG 7 cut(s) 12, 231, 545, 734, 776, 1198, 1515
HpySE526I ACGT 2 cut(s) 1026, 1223
Hsp92I GRCGYC 2 cut(s) 158, 220
Hsp92II CATG 8 cut(s) 117, 254, 334, 595, 731, 863, 1240, 1472
HspAI GCGC 1 cut(s) 709
Ksp22I TGATCA 3 cut(s) 123, 484, 1018
Kzo9I GATC 8 cut(s) 123, 135, 484, 612, 924, 1018, 1422, 1511
LguI GCTCTTC 2 cut(s) 83, 816
LmnI GCTCC 2 cut(s) 1129, 1401
LweI GCATC 3 cut(s) 652, 1065, 1489
MaeI CTAG 4 cut(s) 201, 431, 956, 1190
MaeII ACGT 2 cut(s) 1026, 1223
MaeIII GTNAC 3 cut(s) 139, 409, 1205
MalI GATC 8 cut(s) 125, 137, 486, 614, 926, 1020, 1424, 1513
MbiI CCGCTC 1 cut(s) 368
MboI GATC 8 cut(s) 123, 135, 484, 612, 924, 1018, 1422, 1511
MboII GAAGA 7 cut(s) 100, 115, 803, 896, 934, 1155, 1457
MfeI CAATTG 1 cut(s) 285
MflI RGATCY 2 cut(s) 612, 1511
MhlI GDGCHC 4 cut(s) 95, 800, 971, 1406
MmeI TCCRAC 2 cut(s) 216, 1161
MseI TTAA 8 cut(s) 30, 60, 78, 192, 524, 720, 1085, 1319
MslI CAYNNNNRTG 2 cut(s) 896, 1471
MspA1I CMGCKG 1 cut(s) 172
MspI CCGG 1 cut(s) 1338
MunI CAATTG 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 842
MwoI GCNNNNNNNGC 7 cut(s) 263, 280, 541, 625, 671, 1129, 1347
NdeII GATC 8 cut(s) 123, 135, 484, 612, 924, 1018, 1422, 1511
NlaIII CATG 8 cut(s) 117, 254, 334, 595, 731, 863, 1240, 1472
NlaIV GGNNCC 4 cut(s) 198, 798, 1131, 1403
NmeAIII GCCGAG 1 cut(s) 740
NmuCI GTSAC 2 cut(s) 409, 1205
NspI RCATGY 1 cut(s) 595
PaeR7I CTCGAG 1 cut(s) 666
PciI ACATGT 1 cut(s) 591
PciSI GCTCTTC 2 cut(s) 83, 816
PctI GAATGC 1 cut(s) 842
PfeI GAWTC 3 cut(s) 355, 738, 1266
Pfl23II CGTACG 1 cut(s) 1224
Ple19I CGATCG 1 cut(s) 138
Ppu21I YACGTR 1 cut(s) 1224
PscI ACATGT 1 cut(s) 591
PshBI ATTAAT 1 cut(s) 1319
Psp124BI GAGCTC 1 cut(s) 95
PspFI CCCAGC 1 cut(s) 1350
PspLI CGTACG 1 cut(s) 1224
PspN4I GGNNCC 4 cut(s) 198, 798, 1131, 1403
PspOMI GGGCCC 1 cut(s) 796
PspPI GGNCC 3 cut(s) 197, 796, 797
PspXI VCTCGAGB 1 cut(s) 666
PstI CTGCAG 1 cut(s) 857
PstNI CAGNNNCTG 2 cut(s) 616, 706
PsuI RGATCY 2 cut(s) 612, 1511
PvuI CGATCG 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 172
RsaI GTAC 2 cut(s) 645, 1226
RsaNI GTAC 2 cut(s) 644, 1225
RseI CAYNNNNRTG 2 cut(s) 896, 1471
SacI GAGCTC 1 cut(s) 95
SapI GCTCTTC 2 cut(s) 83, 816
SaqAI TTAA 8 cut(s) 30, 60, 78, 192, 524, 720, 1085, 1319
Sau3AI GATC 8 cut(s) 123, 135, 484, 612, 924, 1018, 1422, 1511
Sau96I GGNCC 3 cut(s) 197, 796, 797
SduI GDGCHC 4 cut(s) 95, 800, 971, 1406
SfaNI GCATC 3 cut(s) 652, 1065, 1489
SfcI CTRYAG 1 cut(s) 853
Sfr274I CTCGAG 1 cut(s) 666
SlaI CTCGAG 1 cut(s) 666
SmiMI CAYNNNNRTG 2 cut(s) 896, 1471
SmlI CTYRAG 4 cut(s) 502, 666, 692, 1498
SmoI CTYRAG 4 cut(s) 502, 666, 692, 1498
SnaBI TACGTA 1 cut(s) 1224
SsiI CCGC 3 cut(s) 164, 368, 1062
SspMI CTAG 4 cut(s) 201, 431, 956, 1190
SstI GAGCTC 1 cut(s) 95
TaaI ACNGT 1 cut(s) 1288
TaiI ACGT 2 cut(s) 1029, 1226
TaqI TCGA 1 cut(s) 667
TatI WGTACW 1 cut(s) 643
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 3 cut(s) 355, 738, 1266
Tru1I TTAA 8 cut(s) 30, 60, 78, 192, 524, 720, 1085, 1319
Tru9I TTAA 8 cut(s) 30, 60, 78, 192, 524, 720, 1085, 1319
TseFI GTSAC 2 cut(s) 409, 1205
Tsp45I GTSAC 2 cut(s) 409, 1205
TspDTI ATGAA 9 cut(s) 62, 122, 239, 481, 596, 803, 840, 1485, 1506
VspI ATTAAT 1 cut(s) 1319
XapI RAATTY 2 cut(s) 937, 1102
XceI RCATGY 1 cut(s) 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 579
XhoI CTCGAG 1 cut(s) 666
XmiI GTMKAC 2 cut(s) 309, 561
XspI CTAG 4 cut(s) 201, 431, 956, 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.