MD06G1206100.v1.1

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002611
Physical Location & Seq
Forward (+)
34085526 .. 34089690
4165 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1206100.v1.1.491

Sequence Viewer

Length: 1590 bp
ATGTTTGTACGAGGAAGAGAGAATATACAGAGATTGATGTGCAAGGTGGAGAAGAAGGAGAGAAAGGGAGTGATGGGATTTAAGGTGTTGGGAGGCGGCGGAGGAGGAGGGGAAAAGTTAAAGAACTCGATGCTGGTCACGGCGGCGTCGTCGCCGTCGAGGTCGAAAATGAAGCTGTGGATGATCCGTGCGACTTCGTCGGTTTTGTTGTGGACGTGCATCGTGCAATTAACGGCTCTGGGGGATATGTGGGGTCCTAGGGTTTTGAAAGGGTGGCCTGCTTGTTTCTCTCTGGAGTCCGCCGCCGCCTCCTCCGTCTTGCAAGACAGGTTGCCTTCCCTTCCGGCCAGAGCTCTTCCGCCCAAGAGGGTTTATAAAAACAATGGTTACCTGATGGTATCATGCAATGGTGGACTCAATCAAATGAGAGCAGCGATATGTGACATGGTTGCTATCGCAAGATATTTGAACGTCACTCTTATAGTCCCTGAACTTGACAAAACCTCCTTTTGGGCTGATCCCAGTGAATTCAAAGACATATTTGATGTGGATCATTTCATCACATCCTTGAGAGATGAGGTTCGCATATTGAAAGAGCTGCCTCCCAGGGTAAAGAGGAGAGTGGAGCTAGGAATGTTTTATACCATGCCACCAGTTAGTTGGTCTGATATGTCTTATTATCGTAATCAGATTCTTCCTCTGATACAAAAATACAAAGTTGTACATCTGAATAGAACTGATGCTCGACTTGCCAATAATGGACAACCTTTGGAGATTCAGAAACTTCGCTGCCGAGTTAATTTCAGTGCTCTGAGATTCACTTCTCAGATAGAGGAGTTGGGCAGAAGGGTTATCAGGCTTCTAAGGCAAAATGGTCCATTCCTTGTACTTCATCTTAGATATGAAATGGACATGTTGGCATTTTCTGGTTGTACCCAAGGTTGTAACATCGAGGAGGTGGAAGAGTTGACAAGAATGAGATATGCTTATCCCTGGTGGAAAGAGAAAATAATAAATTCTGATCTGAAAAGGAAAGATGGTTTGTGTCCTCTGACACCTGAAGAAACCGCTCTTACATTGAGTGCCCTTGACATTGACCGCGATATTCAGATTTATATCGCAGCTGGTGAAATCTATGGCGCAGAGAGGAGAATGGCAAGTCTTCGAGAGGCTTTTCCAAAATTGGTCAGAAAAGAGACACTGCTGGAACCATCAGACCTTAGGTTCTTTCAAAACCACTCATCCCAAATGGCAGCATTGGATTATCTTGTCTCATTGGAGAGTGATATCTTCGTTCCCACATATGATGGAAACATGGCTAAAGTGGTTGAAGGCCATCGCAGGTTTCTTGGGTTCAAGAAGACAATCCTACTGGACAGAAGGCTTCTTGTTGATTTGATAGACCGGTACACTAGTGGATCATTGAATTGGGATGAATTTTCATCTGCGGTAAAGGAAGCTCATGCAAATCGCATGGGGAAACCAACTAATAGGTTGATGATCCAAGACCGACCTAAAGAGGAGGACTATTTCTATGCCAACCCGGAAGAGTGTTTGCAACCATCAGATGATGAACTAAGTAGTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

530

Amino Acids

60.57

Weight (kDa)

9.1

Isoelectric Point (pI)

48.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 130 - 449 3.4e-106 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
Acc36I ACCTGC 1 cut(s) 1332
AccBSI CCGCTC 1 cut(s) 1070
AccII CGCG 1 cut(s) 1101
AclWI GGATC 5 cut(s) 178, 512, 558, 1426, 1495
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 3 cut(s) 527, 1015, 1436
AcuI CTGAAG 1 cut(s) 1080
AcyI GRCGYC 1 cut(s) 146
AfaI GTAC 6 cut(s) 9, 723, 888, 934, 1409, 1585
AfiI CCNNNNNNNGG 1 cut(s) 510
AflIII ACRYGT 1 cut(s) 912
AgeI ACCGGT 1 cut(s) 1404
AgsI TTSAA 8 cut(s) 268, 469, 532, 592, 1232, 1331, 1357, 1426
AhlI ACTAGT 1 cut(s) 1412
AjiI CACGTC 1 cut(s) 216
AjnI CCWGG 2 cut(s) 605, 992
AluBI AGCT 6 cut(s) 175, 353, 598, 628, 1124, 1460
AluI AGCT 6 cut(s) 175, 353, 598, 628, 1124, 1460
Alw21I GWGCWC 2 cut(s) 355, 811
Alw26I GTCTC 2 cut(s) 1190, 1276
AlwI GGATC 5 cut(s) 178, 512, 558, 1426, 1495
AoxI GGCC 3 cut(s) 275, 345, 1333
ApeKI GCWGC 5 cut(s) 431, 598, 789, 1121, 1253
ApoI RAATTY 3 cut(s) 527, 1015, 1436
AsiGI ACCGGT 1 cut(s) 1404
AspA2I CCTAGG 1 cut(s) 257
AspLEI GCGC 1 cut(s) 1142
AspS9I GGNCC 2 cut(s) 254, 875
AsuC2I CCSGG 1 cut(s) 1544
AsuHPI GGTGA 1 cut(s) 1139
AvaII GGWCC 2 cut(s) 254, 875
AvrII CCTAGG 1 cut(s) 257
AxyI CCTNAGG 1 cut(s) 1220
BaeGI GKGCMC 1 cut(s) 1087
BaeI ACNNNNGTAYC 2 cut(s) 1399, 1432
BanII GRGCYC 1 cut(s) 355
BbsI GAAGAC 2 cut(s) 1154, 1367
Bbv12I GWGCWC 2 cut(s) 355, 811
BbvI GCAGC 5 cut(s) 443, 585, 776, 1133, 1265
BccI CCATC 7 cut(s) 67, 388, 1031, 1219, 1301, 1344, 1570
BceAI ACGGC 3 cut(s) 139, 156, 249
BciT130I CCWGG 2 cut(s) 607, 994
BcnI CCSGG 1 cut(s) 1544
BcoDI GTCTC 2 cut(s) 1190, 1276
BcuI ACTAGT 1 cut(s) 1412
BfaI CTAG 3 cut(s) 258, 629, 1413
BfuAI ACCTGC 1 cut(s) 1332
BisI GCNGC 9 cut(s) 97, 144, 303, 306, 432, 599, 790, 1122, 1254
BlnI CCTAGG 1 cut(s) 257
BlsI GCNGC 9 cut(s) 98, 145, 304, 307, 433, 600, 791, 1123, 1255
Bme1390I CCNGG 3 cut(s) 607, 994, 1544
Bme18I GGWCC 2 cut(s) 254, 875
BmgBI CACGTC 1 cut(s) 216
BmgT120I GGNCC 2 cut(s) 254, 875
BmiI GGNNCC 2 cut(s) 255, 1209
BmrFI CCNGG 3 cut(s) 607, 994, 1544
BmrI ACTGGG 1 cut(s) 516
BmsI GCATC 3 cut(s) 120, 228, 730
BmuI ACTGGG 1 cut(s) 516
BpiI GAAGAC 2 cut(s) 1154, 1367
BpmI CTGGAG 1 cut(s) 314
BpuEI CTTGAG 1 cut(s) 589
BpuMI CCSGG 1 cut(s) 1544
BsaAI YACGTR 1 cut(s) 1587
BsaBI GATNNNNATC 2 cut(s) 549, 1115
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 5 cut(s) 257, 605, 606, 937, 992
BsaWI WCCGGW 1 cut(s) 1404
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse118I RCCGGY 1 cut(s) 1404
Bse1I ACTGG 3 cut(s) 522, 653, 1377
Bse21I CCTNAGG 1 cut(s) 1220
Bse3DI GCAATG 1 cut(s) 412
Bse8I GATNNNNATC 2 cut(s) 549, 1115
BseBI CCWGG 2 cut(s) 607, 994
BseDI CCNNGG 5 cut(s) 257, 605, 606, 937, 992
BseGI GGATG 4 cut(s) 186, 563, 1241, 1438
BseJI GATNNNNATC 2 cut(s) 549, 1115
BseLI CCNNNNNNNGG 1 cut(s) 510
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 2 cut(s) 803, 839
BseNI ACTGG 3 cut(s) 522, 653, 1377
BseRI GAGGAG 8 cut(s) 117, 120, 301, 631, 848, 968, 1162, 1535
BseSI GKGCMC 1 cut(s) 1087
BseXI GCAGC 5 cut(s) 443, 585, 776, 1133, 1265
Bsh1236I CGCG 1 cut(s) 1101
BshFI GGCC 3 cut(s) 277, 347, 1335
BshTI ACCGGT 1 cut(s) 1404
BsiHKAI GWGCWC 2 cut(s) 355, 811
BsiSI CCGG 3 cut(s) 344, 1405, 1544
BslFI GGGAC 1 cut(s) 470
BslI CCNNNNNNNGG 1 cut(s) 510
BsmAI GTCTC 2 cut(s) 1190, 1276
BsmFI GGGAC 1 cut(s) 470
BsnI GGCC 3 cut(s) 277, 347, 1335
Bsp1286I GDGCHC 3 cut(s) 355, 811, 1087
Bsp1407I TGTACA 1 cut(s) 721
Bsp143I GATC 6 cut(s) 183, 517, 550, 1021, 1418, 1500
BspANI GGCC 3 cut(s) 277, 347, 1335
BspCNI CTCAG 2 cut(s) 804, 838
BspFNI CGCG 1 cut(s) 1101
BspLI GGNNCC 2 cut(s) 255, 1209
BspMI ACCTGC 1 cut(s) 1332
BspPI GGATC 5 cut(s) 178, 512, 558, 1426, 1495
BspQI GCTCTTC 1 cut(s) 360
BsrBI CCGCTC 1 cut(s) 1070
BsrDI GCAATG 1 cut(s) 412
BsrFI RCCGGY 1 cut(s) 1404
BsrGI TGTACA 1 cut(s) 721
BsrI ACTGG 3 cut(s) 522, 653, 1377
BssAI RCCGGY 1 cut(s) 1404
BssECI CCNNGG 5 cut(s) 257, 605, 606, 937, 992
BssMI GATC 6 cut(s) 183, 517, 550, 1021, 1418, 1500
BssNI GRCGYC 1 cut(s) 146
BssT1I CCWWGG 2 cut(s) 257, 937
Bst2UI CCWGG 2 cut(s) 607, 994
Bst6I CTCTTC 4 cut(s) 10, 360, 957, 1542
BstACI GRCGYC 1 cut(s) 146
BstAUI TGTACA 1 cut(s) 721
BstBAI YACGTR 1 cut(s) 1587
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 6 cut(s) 812, 825, 863, 896, 1220, 1577
BstEII GGTNACC 1 cut(s) 386
BstF5I GGATG 4 cut(s) 186, 563, 1241, 1438
BstFNI CGCG 1 cut(s) 1101
BstHHI GCGC 1 cut(s) 1142
BstKTI GATC 6 cut(s) 186, 520, 553, 1024, 1421, 1503
BstMAI GTCTC 2 cut(s) 1190, 1276
BstMBI GATC 6 cut(s) 183, 517, 550, 1021, 1418, 1500
BstMWI GCNNNNNNNGC 2 cut(s) 749, 865
BstNI CCWGG 2 cut(s) 607, 994
BstNSI RCATGY 1 cut(s) 916
BstPI GGTNACC 1 cut(s) 386
BstSCI CCNGG 3 cut(s) 605, 992, 1542
BstSLI GKGCMC 1 cut(s) 1087
BstUI CGCG 1 cut(s) 1101
BstV1I GCAGC 5 cut(s) 443, 585, 776, 1133, 1265
BstV2I GAAGAC 2 cut(s) 1154, 1367
BstXI CCANNNNNNTGG 1 cut(s) 660
Bsu36I CCTNAGG 1 cut(s) 1220
BsuRI GGCC 3 cut(s) 277, 347, 1335
BtgZI GCGATG 1 cut(s) 1322
BtrI CACGTC 1 cut(s) 216
BtsCI GGATG 4 cut(s) 186, 563, 1241, 1438
BtsI GCAGTG 1 cut(s) 1199
BtsIMutI CAGTG 3 cut(s) 529, 811, 1199
BveI ACCTGC 1 cut(s) 1332
Cac8I GCNNGC 1 cut(s) 279
CfoI GCGC 1 cut(s) 1142
Cfr10I RCCGGY 1 cut(s) 1404
Cfr13I GGNCC 2 cut(s) 254, 875
CseI GACGC 1 cut(s) 135
Csp6I GTAC 6 cut(s) 8, 722, 887, 933, 1408, 1584
CspAI ACCGGT 1 cut(s) 1404
CviAII CATG 7 cut(s) 402, 445, 646, 913, 1315, 1463, 1474
CviQI GTAC 6 cut(s) 8, 722, 887, 933, 1408, 1584
DdeI CTNAG 6 cut(s) 812, 825, 863, 896, 1220, 1577
DpnI GATC 6 cut(s) 185, 519, 552, 1023, 1420, 1502
DpnII GATC 6 cut(s) 183, 517, 550, 1021, 1418, 1500
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 4 cut(s) 10, 360, 957, 1542
EarI CTCTTC 4 cut(s) 10, 360, 957, 1542
EciI GGCGGA 3 cut(s) 114, 289, 348
Ecl136II GAGCTC 1 cut(s) 353
Eco130I CCWWGG 2 cut(s) 257, 937
Eco24I GRGCYC 1 cut(s) 355
Eco32I GATATC 1 cut(s) 1288
Eco47I GGWCC 2 cut(s) 254, 875
Eco53kI GAGCTC 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 1080
Eco81I CCTNAGG 1 cut(s) 1220
Eco91I GGTNACC 1 cut(s) 386
EcoICRI GAGCTC 1 cut(s) 353
EcoO109I RGGNCCY 1 cut(s) 254
EcoO65I GGTNACC 1 cut(s) 386
EcoRI GAATTC 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 605, 992
EcoRV GATATC 1 cut(s) 1288
EcoT14I CCWWGG 2 cut(s) 257, 937
EcoT38I GRGCYC 1 cut(s) 355
ErhI CCWWGG 2 cut(s) 257, 937
FaeI CATG 7 cut(s) 405, 448, 649, 916, 1318, 1466, 1477
FaqI GGGAC 1 cut(s) 470
FatI CATG 7 cut(s) 401, 444, 645, 912, 1314, 1462, 1473
FauNDI CATATG 1 cut(s) 1303
Fnu4HI GCNGC 9 cut(s) 97, 144, 303, 306, 432, 599, 790, 1122, 1254
FokI GGATG 4 cut(s) 193, 550, 1228, 1445
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 9 cut(s) 97, 144, 303, 306, 432, 599, 790, 1122, 1254
FspBI CTAG 3 cut(s) 258, 629, 1413
GlaI GCGC 1 cut(s) 1141
GluI GCNGC 9 cut(s) 97, 144, 303, 306, 432, 599, 790, 1122, 1254
GsuI CTGGAG 1 cut(s) 314
HaeIII GGCC 3 cut(s) 277, 347, 1335
HapII CCGG 3 cut(s) 344, 1405, 1544
HgaI GACGC 1 cut(s) 135
HhaI GCGC 1 cut(s) 1142
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 7 cut(s) 405, 448, 649, 916, 1318, 1466, 1477
Hin6I GCGC 1 cut(s) 1140
HinP1I GCGC 1 cut(s) 1140
HincII GTYRAC 1 cut(s) 969
HindII GTYRAC 1 cut(s) 969
HinfI GANTC 5 cut(s) 296, 414, 691, 775, 816
HpaII CCGG 3 cut(s) 344, 1405, 1544
HphI GGTGA 1 cut(s) 1139
Hpy166II GTNNAC 4 cut(s) 213, 413, 969, 1410
Hpy188III TCNNGA 3 cut(s) 293, 1166, 1357
Hpy8I GTNNAC 4 cut(s) 213, 413, 969, 1410
Hpy99I CGWCG 4 cut(s) 151, 154, 160, 202
HpyAV CCTTC 6 cut(s) 49, 345, 350, 840, 1325, 1374
HpyCH4IV ACGT 3 cut(s) 215, 471, 1586
HpyCH4V TGCA 7 cut(s) 42, 219, 226, 322, 405, 1466, 1558
HpyF10VI GCNNNNNNNGC 2 cut(s) 749, 865
HpyF3I CTNAG 6 cut(s) 812, 825, 863, 896, 1220, 1577
HpySE526I ACGT 3 cut(s) 215, 471, 1586
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 7 cut(s) 405, 448, 649, 916, 1318, 1466, 1477
HspAI GCGC 1 cut(s) 1140
Kzo9I GATC 6 cut(s) 183, 517, 550, 1021, 1418, 1500
LguI GCTCTTC 1 cut(s) 360
LmnI GCTCC 1 cut(s) 625
Lsp1109I GCAGC 5 cut(s) 443, 585, 776, 1133, 1265
LweI GCATC 3 cut(s) 120, 228, 730
MaeI CTAG 3 cut(s) 258, 629, 1413
MaeII ACGT 3 cut(s) 215, 471, 1586
MaeIII GTNAC 5 cut(s) 136, 386, 440, 472, 944
MalI GATC 6 cut(s) 185, 519, 552, 1023, 1420, 1502
MbiI CCGCTC 1 cut(s) 1070
MboI GATC 6 cut(s) 183, 517, 550, 1021, 1418, 1500
MhlI GDGCHC 3 cut(s) 355, 811, 1087
MluCI AATT 7 cut(s) 227, 527, 799, 1015, 1181, 1426, 1436
MlyI GAGTC 2 cut(s) 305, 408
MseI TTAA 4 cut(s) 81, 119, 230, 798
MspA1I CMGCKG 1 cut(s) 1124
MspI CCGG 3 cut(s) 344, 1405, 1544
MspR9I CCNGG 3 cut(s) 607, 994, 1544
MvaI CCWGG 2 cut(s) 607, 994
MvnI CGCG 1 cut(s) 1101
MwoI GCNNNNNNNGC 2 cut(s) 749, 865
NciI CCSGG 1 cut(s) 1544
NdeI CATATG 1 cut(s) 1303
NdeII GATC 6 cut(s) 183, 517, 550, 1021, 1418, 1500
NlaIII CATG 7 cut(s) 405, 448, 649, 916, 1318, 1466, 1477
NlaIV GGNNCC 2 cut(s) 255, 1209
NmeAIII GCCGAG 1 cut(s) 818
NmuCI GTSAC 3 cut(s) 136, 440, 472
NspI RCATGY 1 cut(s) 916
PasI CCCWGGG 1 cut(s) 606
PciI ACATGT 1 cut(s) 912
PciSI GCTCTTC 1 cut(s) 360
PcsI WCGNNNNNNNCGW 2 cut(s) 146, 155
PfeI GAWTC 3 cut(s) 691, 775, 816
PflFI GACNNNGTC 1 cut(s) 196
PinAI ACCGGT 1 cut(s) 1404
PkrI GCNGC 9 cut(s) 98, 145, 304, 307, 433, 600, 791, 1123, 1255
PleI GAGTC 2 cut(s) 304, 408
PpsI GAGTC 2 cut(s) 304, 408
Ppu21I YACGTR 1 cut(s) 1587
PpuMI RGGWCCY 1 cut(s) 254
PscI ACATGT 1 cut(s) 912
PsiI TTATAA 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 355
Psp5II RGGWCCY 1 cut(s) 254
Psp6I CCWGG 2 cut(s) 605, 992
PspEI GGTNACC 1 cut(s) 386
PspGI CCWGG 2 cut(s) 605, 992
PspN4I GGNNCC 2 cut(s) 255, 1209
PspPI GGNCC 2 cut(s) 254, 875
PspPPI RGGWCCY 1 cut(s) 254
PsyI GACNNNGTC 1 cut(s) 196
PvuII CAGCTG 1 cut(s) 1124
RsaI GTAC 6 cut(s) 9, 723, 888, 934, 1409, 1585
RsaNI GTAC 6 cut(s) 8, 722, 887, 933, 1408, 1584
SacI GAGCTC 1 cut(s) 355
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 4 cut(s) 81, 119, 230, 798
SatI GCNGC 9 cut(s) 97, 144, 303, 306, 432, 599, 790, 1122, 1254
Sau3AI GATC 6 cut(s) 183, 517, 550, 1021, 1418, 1500
Sau96I GGNCC 2 cut(s) 254, 875
SchI GAGTC 2 cut(s) 305, 408
ScrFI CCNGG 3 cut(s) 607, 994, 1544
SduI GDGCHC 3 cut(s) 355, 811, 1087
SfaNI GCATC 3 cut(s) 120, 228, 730
SinI GGWCC 2 cut(s) 254, 875
SmlI CTYRAG 1 cut(s) 568
SmoI CTYRAG 1 cut(s) 568
SpeI ACTAGT 1 cut(s) 1412
Sse9I AATT 7 cut(s) 227, 527, 799, 1015, 1181, 1426, 1436
SspMI CTAG 3 cut(s) 258, 629, 1413
SstI GAGCTC 1 cut(s) 355
StyD4I CCNGG 3 cut(s) 605, 992, 1542
StyI CCWWGG 2 cut(s) 257, 937
TaiI ACGT 3 cut(s) 218, 474, 1589
TaqI TCGA 6 cut(s) 128, 158, 164, 745, 951, 1165
TaqII GACCGA 1 cut(s) 1524
TasI AATT 7 cut(s) 227, 527, 799, 1015, 1181, 1426, 1436
TatI WGTACW 2 cut(s) 721, 886
TauI GCSGC 4 cut(s) 99, 146, 305, 308
TfiI GAWTC 3 cut(s) 691, 775, 816
Tru1I TTAA 4 cut(s) 81, 119, 230, 798
Tru9I TTAA 4 cut(s) 81, 119, 230, 798
TscAI CASTG 3 cut(s) 529, 811, 1206
TseFI GTSAC 3 cut(s) 136, 440, 472
TseI GCWGC 5 cut(s) 431, 598, 789, 1121, 1253
Tsp45I GTSAC 3 cut(s) 136, 440, 472
TspDTI ATGAA 7 cut(s) 185, 547, 881, 918, 1431, 1449, 1587
TspGWI ACGGA 2 cut(s) 176, 304
TspRI CASTG 3 cut(s) 529, 811, 1206
Tth111I GACNNNGTC 1 cut(s) 196
VpaK11BI GGWCC 2 cut(s) 254, 875
XapI RAATTY 3 cut(s) 527, 1015, 1436
XceI RCATGY 1 cut(s) 916
XcmI CCANNNNNNNNNTGG 1 cut(s) 657
XmaJI CCTAGG 1 cut(s) 257
XspI CTAG 3 cut(s) 258, 629, 1413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.